Eukaryotic translation initiation factor 2-alpha kinase 4

Overview


Name	Eukaryotic translation initiation factor 2-alpha kinase 4
Smed ID	SMED30032249
Length (bp)	5198

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Eukaryotic translation initiation factor 2-alpha kinase 4 (SMED30032249) t-SNE clustered cells

Violin plots show distribution of expression levels for Eukaryotic translation initiation factor 2-alpha kinase 4 (SMED30032249) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Eukaryotic translation initiation factor 2-alpha kinase 4 (SMED30032249) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30032249

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 2

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication	Specimen	Lifecycle	Evidence
X1 cell	SMED30032249	h1SMcG0001796	SmedASXL_018287	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
parenchyma	SMED30032249	h1SMcG0001796	dd_Smed_v4_8210_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

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Homology

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Human
Match: EIF2AK4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:HGNC Symbol;Acc:HGNC:19687])

HSP 1 Score: 343.584 bits (880), Expect = 2.263e-95
Identity = 395/1551 (25.47%), Postives = 665/1551 (42.88%), Query Frame = 1

Query:  124 QSDEVKALRSIFGKEFKLIRPKNLKKKYEPPLVSVLI---NVDSSNLNSALSLRISFDCPQNYPFDIPKISIYDAVNVPTNLVEQLLIILNEKSNLLKGYSMIYNLVEEARCIIIKHFETDHKSVNSILTINKVLNEDE-IMQTETKQSEFLKNLLRQRRDS-------------ARXXXXXXXXXXXCDHSYKTDELEFEHCSDRSSGCLVYKGICDGQHRI------------------------------------------KTINPNMQIKLNTFNGFNRQKGTNVKIYQI---------RFSDLKRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKR---------FEELKGRGHSNERTGASTIKKGSKRYDLMKLGVLAKEL--------FFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFLTCE---NLSKNSRQNSQTVDSAQDLTPWHLFEKS----------SRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSSAISVIKPETTDSLLLDFQDKKNSIMFSANVNFSQIEHCSRHFDLESFXXXXXXXXXXXXVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPFHGNK----TKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVEN--GPVGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEII-----FPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLK--RFVTRYIESIFVSHGAVEFLAPLLSPK----FINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTD-YDSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRAS--------LHDMVSKDVKNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNRIHEGYDVINSAFKELKSVEEKLKHFTEDLCFCVNFRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEI-YDKDEHGEE 4374
            Q  E++AL +I+G +F+ +RP       EPP +++++    +    +   + LR+   CP  YP  +P+I + +A  +    V  L   L E +    G  MI+ L    +  + +H +   KS +  +   +   E + +++ + K+ +  + +L + +               A+ E+         DH+ K D       +    G   + G  +G+HR                                           K I  + Q+    +N      G  V +Y+           F   + K+  D     +   E +F  L KL+HP+++ Y     +  +  I      D+++++++    G+ L           +   +    + + ++  Y   +L  + YLH  ++    ++  ++L+D +  +KI ++ I KR         FE+ + R   N    A   K G K+ D+ +LG+L   L        +   IP      +D+P+D  DF+  C         + ++L  H F+  +    L + S ++S+  D  + + P +    +          SR   EFE +  LG+GAFG V+KV+N  D   YA+KR+ +N   ++  R   EV LLS L+HENIVRY+ +W E  ++ +       P  DS  +  +  +A    +P +    L   +      + S++V +S     S       F  +   S  D   +  +    + Q  +  S   SD   +  D + K    DE               + ++ N  H ++    T+   YL IQME CE   +TL+  I DQ LY +   +WR  REI+ GL +IH K MIHRDLKP NIFLDS   VKIGD GLA +      +S   +   D   S+   +  G VGT +Y+ PE+    +S+Y  K D++SLGII FEM + P  T SER  +L  LR P        F D +H K KS    +I  LLNHD   RP+A+ELL+S ++PPP+ +E EL + L     N     Y+ M+ ++FS+  S    Y                              S I +G+F   T K  + V   I  IF  HGAV+   PLL P+    + + E+  F  +D  G++V LP +LR+ FA ++  N I   KRY I  +++ + + R HP+E +E AFDIVT   +S   TAEI+Y +++I ++    +    ++ +N+T L  A++ H  I           L+D V++ +   + +      +L+      +   +   G  L+DL         +      ++    K+L+ V   LK     L   +N    LV     ++G++FQ        F+  IK  RR + +          +IL++GG Y   + + + P     G V    +A+G SI +  +  ++ ++E+ +     D+ V    +  M+ A+N               L  +LW A I+AEI YD  +  EE
Sbjct:   23 QDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVK--CPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQRRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVG--NGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYFNMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVRYLAMNLKEQDDSIV----VDILVEHIS----GVSL-----------AAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRLADICKEDVFEQTRVRFSDN----ALPYKTG-KKGDVWRLGLLLLSLSQGQECGEYPVTIP------SDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGT---PPPDSGPLAKDDRAARG--QPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSAS---ARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQDE---------------DCNEKNGCHESEPSVTTEAVHYLYIQMEYCEK--STLRDTI-DQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKS----VISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYT---------------------------YDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALF--MDHSGMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGD-LQDLMPTINSLIKQKTGIAQLVKYGLKDLEEVVGLLKKLGIKLQVLINL--GLVYKVQQHNGIIFQ--------FVAFIK--RRQRAVP---------EILAAGGRYDLLIPQFRGPQA--LGPV---PTAIGVSIAIDKISAAVLNMEESVTISSCDLLVVSVGQMSMSRAIN---------------LTQKLWTAGITAEIMYDWSQSQEE 1438

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Human
Match: EIF2AK4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:HGNC Symbol;Acc:HGNC:19687])

HSP 1 Score: 249.21 bits (635), Expect = 5.367e-66
Identity = 222/716 (31.01%), Postives = 340/716 (47.49%), Query Frame = 1

Query: 2302 QMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVEN--GPVGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEII-----FPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLK--RFVTRYIESIFVSHGAVEFLAPLLSPK----FINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTD-YDSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRAS--------LHDMVSKDVKNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNRIHEGYDVINSAFKELKSVEEKLKHFTEDLCFCVNFRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEI-YDKDEHGEE 4374
            QME CE   +TL+  ID Q LY +   +WR  REI+ GL +IH K MIHRDLKP NIFLDS   VKIGD GLA +      +S   +   D   S+   +  G VGT +Y+ PE+    +S+Y  K D++SLGII FEM + P  T SER  +L  LR P        F D +H K KS    +I  LLNHD   RP+A+ELL+S ++PPP+ +E EL + L     N     Y+ M+ ++FS+  S    Y                              S I +G+F   T K  + V   I  IF  HGAV+   PLL P+    + + E+  F  +D  G++V LP +LR+ FA ++  N I   KRY I  +++ + + R HP+E +E AFDIVT   +S   TAEI+Y +++I ++    +    ++ +N+T L  A++ H  I           L+D V++ +   + +      +L+      +   +   G  L+DL         +      ++    K+L+ V   LK     L   +N    LV     ++G++FQ        F+  IK  RR + +          +IL++GG Y   + + + P     G V    +A+G SI +  +  ++ ++E+ +     D+ V    +  M+ A+N               L  +LW A I+AEI YD  +  EE
Sbjct:  579 QMEYCEK--STLRDTID-QGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKS----VISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYD---------------------------SDILKGNFSIRTAKMQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALF--MDHSGMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGD-LQDLMPTINSLIKQKTGIAQLVKYGLKDLEEVVGLLKKLGIKLQVLINL--GLVYKVQQHNGIIFQ--------FVAFIK--RRQRAVP---------EILAAGGRYDLLIPQFRGP--QALGPV---PTAIGVSIAIDKISAAVLNMEESVTISSCDLLVVSVGQMSMSRAIN---------------LTQKLWTAGITAEIMYDWSQSQEE 1216

HSP 2 Score: 101.293 bits (251), Expect = 1.184e-20
Identity = 102/427 (23.89%), Postives = 186/427 (43.56%), Query Frame = 1

Query:  733 HRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQI---------RFSDLKRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKR---------FEELKGRGHSNERTGASTIKKGSKRYDLMKLGVLAKELFFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFLTCEN---LSKNSRQNSQTVDSAQDLTPWHLFEKS----------SRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSSA 1899
            H+ K I  + Q+    +N      G  V +Y+           F   + K+  D     +   E +F  L KL+HP+++ Y     +  +  I      D+++++++    G+ L           +   +    + + ++  Y   +L  + YLH  ++    ++  ++L+D +  +KI ++ I KR         FE+ + R  S+      T KKG      + L  L++       P T    +D+P+D  DF+  C         + ++L  H F+  +    L + S ++S+  D  + + P +    +          SR   EFE +  LG+GAFG V+KV+N  D   YA+KR+ +N   ++  R   EV LLS L+HENIVRY+ +W E  ++ +       P  DS  +  +  +A
Sbjct:   87 HKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVRYLAMNLKEQDDSIV----VDILVEHIS----GVSL-----------AAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRLADICKEDVFEQTRVR-FSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTI--PSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGT---PPPDSGPLAKDDRAA 488

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Human
Match: EIF2AK1 (eukaryotic translation initiation factor 2 alpha kinase 1 [Source:HGNC Symbol;Acc:HGNC:24921])

HSP 1 Score: 159.844 bits (403), Expect = 9.579e-40
Identity = 141/520 (27.12%), Postives = 242/520 (46.54%), Query Frame = 1

Query: 1456 SCNSNIEELKLHPFLTCENLSKNSRQNSQTVDSAQDLTPWH--------LFEKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRL-NAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQ-------KSSSETNNLQ------PNIDSSSIRNNTSSAISVIKPETTDSLLLDF--QDKKN----SIMFSANVNFSQIEHCSRHFDLESFXXXXXXXXXXXXVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPFHGNKTKIEKYLM--IQMELCE--------GQYNTLQKYIDDQKL-YMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDS-QKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNI 2895
            +C+     L+LH      +L +++++  +  D  +D++           L  ++SR   EFE +  LG+G +G V KVRN  D QYYAIK++ +  A K    + ++EV++L+ L H NIV Y  +W E +        +++ E  +L+       + +   ++N+ SS+ S+I  E T      F   D +N    S+ ++ N+   +        +L+    +   + S     ++ Q P  +   +E SF  ++                                +  +N  F G +T+ + +LM  IQM+LCE         +    ++Y+D+    Y+   +  +  +E+V G+ +IH+  ++HRDLKP NIFL    ++VKIGD GLA           N ++   NG         VGT +Y  PE L  S Y  K+DMYSLG+++ E+  +PFGT  ER  +L  LR  ++     K   +++ +   I+ L   +   RPSA +LLQS +
Sbjct:  108 TCSDEFSSLRLHHNRAITHLMRSAKERVRQ-DPCEDISRIQKIRSREVALEAQTSRYLNEFEELAILGKGGYGRVYKVRNKLDGQYYAIKKILIKGATKTVCMKVLREVKVLAGLQHPNIVGYHTAWIEHVHVIQPRADRAAIELPSLEVLSDQEEDREQCGVKNDESSSSSIIFAEPTPEKEKRFGESDTENQNNKSVKYTTNLVIRESGELESTLELQENGLAGLSASSI----VEQQLPLRRNSHLEESFTSTEE-------------------------------SSEENVNFLG-QTEAQYHLMLHIQMQLCELSLWDWIVERNKRGREYVDESACPYVMANVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACTD----ILQKNTDWTNRNGKRTPTHTSRVGTCLYASPEQLEGSEYDAKSDMYSLGVVLLEL-FQPFGTEMERAEVLTGLRTGQLPESLRKRCPVQAKY---IQHLTRRNSSQRPSAIQLLQSEL 582

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Human
Match: NEK3 (NIMA related kinase 3 [Source:HGNC Symbol;Acc:HGNC:7746])

HSP 1 Score: 97.8265 bits (242), Expect = 2.808e-21
Identity = 73/238 (30.67%), Postives = 113/238 (47.48%), Query Frame = 1

Query: 2284 EKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMC--HKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIP-------PPEGKELELRKFLIDVTKNSSSN 2970
            E +L I ME C+G  + +QK    +     E +I  +  ++  G+ HIH K ++HRD+K  NIFL    +VK+GD G A   +N +  +                   VGT  Y+ PE+     Y NK+D++SLG I++E+C    PF   S +  IL   +    I P   H+  +  F  L+K +   +   RPSA+ LL   I+        PPE   +E  + +++  KNS  N
Sbjct:   72 EGHLYIVMEYCDGG-DLMQKIKQQKGKLFPEDMILNWFTQMCLGVNHIHKKRVLHRDIKSKNIFLTQNGKVKLGDFGSARLLSNPMAFACTY----------------VGTPYYVPPEIWENLPYNNKSDIWSLGCILYELCTLKHPFQANSWKNLILKVCQ--GCISPLPSHYSYELQF--LVKQMFKRNPSHRPSATTLLSRGIVARLVQKCLPPE-IIMEYGEEVLEEIKNSKHN 287

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Human
Match: NEK6 (NIMA related kinase 6 [Source:HGNC Symbol;Acc:HGNC:7749])

HSP 1 Score: 93.9745 bits (232), Expect = 8.651e-21
Identity = 46/139 (33.09%), Postives = 83/139 (59.71%), Query Frame = 1

Query: 2293 LMIQMELCE-GQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEM 2706
            L I +EL + G  + + KY   QK  + E+ +W++  ++ S +EH+HS+ ++HRD+KP N+F+ +   VK+GDLGL   F+++ T + +                 VGT  Y+ PE ++++ Y  K+D++SLG +++EM
Sbjct:   50 LNIVLELADAGDLSQMIKYFKKQKRLIPERTVWKYFVQLCSAVEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSETTAAHSL----------------VGTPYYMSPERIHENGYNFKSDIWSLGCLLYEM 172

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Celegans
Match: gcn-2 (Eukaryotic translation initiation factor 2-alpha kinase gcn-2 [Source:UniProtKB/Swiss-Prot;Acc:D0Z5N4])

HSP 1 Score: 167.548 bits (423), Expect = 3.219e-41
Identity = 186/751 (24.77%), Postives = 311/751 (41.41%), Query Frame = 1

Query: 2287 KYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGL----AMNFNNKVTESANQE------YVKDNGL---SNDV-ENGPVGTIMYICP-----EMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVEL----IKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSL---LYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPKFINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIV-TDYDSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHY----SIRASLHDMVSK-DVKNLDSDHFRSLTNLTDK---QKANIKSILLFNGSRLEDLEKHFKIFQNRIH---------EGYDVINSAFKELKSVEEKLK----HFTEDLCFCVNFRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVN----------VVVQYDIEMDG--II---WDLVSRLWAANISAEIY 4350
            + L IQME C+    TL++YID+   +     +WR   E++ GL+++H   MIHRD+KP NIFL SQ  VKIGD GL    AM+   K+   A ++       +  NG+    +DV +   +GT +Y+ P     E+++++ YT+K D+YS G+++FEM ++P     +R   L NLR  +I  P +    + +    L    ++ +L  +   RP+A +LL    +P    ++   R     V K     +   +L + F +       Y   +   L ++ +N+     + ++   LR                +  F   +  ++     A+   +    P           +LD  GV V LP +LR NF  F V N +   KR+  G +Y Q +    HP ER E   D +     SP+  AE+L +  ++   S        TL+I +  L  A + H      +RA L D +    V +    H   +  LT K   + ANI + LL        +E +F  F+ ++              +++ A ++L+ +    K       E +    + +    P  T   GL+FQI          V KP     N R         Q + +GG Y S L++ + P          FV                Y I   I  FGV + V       +N + N          V++   ++ DG  +I   ++L  +LW+  I A+++
Sbjct:  776 RILCIQMEYCD--RATLRQYIDENHCFNAPTEVWRIFSEVLCGLKYMHDMAMIHRDIKPLNIFLTSQNGVKIGDFGLATLEAMSSKGKIVGGAAEKSTSIEAMLSPNGVKSKGSDVHQTRDIGTQLYMAPELFVDELVHKAPYTSKIDIYSAGVVLFEMFYRPLPPSMDRVSTLNNLRD-DIKIPSDFGAGLAAPMAGLARRTVEKMLQRNPDERPTADDLLNDEDLPMHTKEDATFRNLCEKVIKKRDGRMNAWLLDKQFKEEVPTSLNYCYDVDICLERAKYNN-----REVLVETLRAEFCK--------ILKIHSFEKLHTHTLMPVSTALAAASVRTKP---------VEVLDRSGVPVALPMDLRQNFVRFCVRNSVQRMKRFNFGRVYSQTSA-NGHPHERWECCVDCIGPQCSSPSLEAELLLVACEMMIGSLPG--MKFTLKIGHAQLIEAQIRHLKLSDDVRAELLDALHLISVSDRPHSHKEKMDMLTPKIGAKAANIITKLLI------PVEDNFGAFKEKVACFRKKLKVDAARVLVDKAIRDLEEIVGTFKFCRTEAIEQISIVYDSQTCYRP-RTFGDGLLFQI---------QVEKPSTIANNKR------GRRQNVLAGGRYDSALLRERHP--------RDFV----------------YEIPLCISGFGVAMDVVSQIRDSINKSANIPKTPQNHCKVLICSMVQPDGSNLITQKFELAKKLWSMGIEADVF 1452

HSP 2 Score: 78.1814 bits (191), Expect = 5.510e-14
Identity = 60/184 (32.61%), Postives = 91/184 (49.46%), Query Frame = 1

Query: 1339 KRYDLMKLGVLAKELF-------FFGIPETEREETDMPSDLI-DFITSCNS--NIEELKLHPFLT--CENLSKNSRQNSQTVDSAQDLTPWH-LFEKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTM-KEVQLLSSLNHENIVRYFASWSEEMQKSSSETNN 1848
            +R DL +LG L   L        +  +P       +  ++L+ +FI  C    NI++L   PFL   C++ S+N              TP+        R+  +  +I  LG G FGDVV VRN  D+  YAIKR+ LNA   K  R + KE +  + LNH N+VRY+ +W+E++     ET++
Sbjct:  399 RRKDLFQLGTLLDGLILATRGSTYSRVPTPVEGNQNTGTNLLGNFIAKCQEAKNIDQLVEDPFLKEECQSESENI------------FTPFGGAMSPDGRMLADNVIIRVLGRGGFGDVVLVRNKMDSTDYAIKRIPLNAKSDKLNRKIAKEAKFFAKLNHPNMVRYYYAWAEDLIPIVEETSD 570

[Close]

Query: 2287 KYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGL----AMNFNNKVTESANQE------YVKDNGL---SNDV-ENGPVGTIMYICP-----EMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVEL----IKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSL---LYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPKFINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIV-TDYDSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHY----SIRASLHDMVSK-DVKNLDSDHFRSLTNLTDK---QKANIKSILLFNGSRLEDLEKHFKIFQNRIH---------EGYDVINSAFKELKSVEEKLK----HFTEDLCFCVNFRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVN----------VVVQYDIEMDG--II---WDLVSRLWAANISAEIY 4350
            + L IQME C+    TL++YID+   +     +WR   E++ GL+++H   MIHRD+KP NIFL SQ  VKIGD GL    AM+   K+   A ++       +  NG+    +DV +   +GT +Y+ P     E+++++ YT+K D+YS G+++FEM ++P     +R   L NLR  +I  P +    + +    L    ++ +L  +   RP+A +LL    +P    ++   R     V K     +   +L + F +       Y   +   L ++ +N+     + ++   LR                +  F   +  ++     A+   +    P           +LD  GV V LP +LR NF  F V N +   KR+  G +Y Q +    HP ER E   D +     SP+  AE+L +  ++   S        TL+I +  L  A + H      +RA L D +    V +    H   +  LT K   + ANI + LL        +E +F  F+ ++              +++ A ++L+ +    K       E +    + +    P  T   GL+FQI          V KP     N R         Q + +GG Y S L++ + P          FV                Y I   I  FGV + V       +N + N          V++   ++ DG  +I   ++L  +LW+  I A+++
Sbjct:  776 RILCIQMEYCD--RATLRQYIDENHCFNAPTEVWRIFSEVLCGLKYMHDMAMIHRDIKPLNIFLTSQNGVKIGDFGLATLEAMSSKGKIVGGAAEKSTSIEAMLSPNGVKSKGSDVHQTRDIGTQLYMAPELFVDELVHKAPYTSKIDIYSAGVVLFEMFYRPLPPSMDRVSTLNNLRD-DIKIPSDFGAGLAAPMAGLARRTVEKMLQRNPDERPTADDLLNDEDLPMHTKEDATFRNLCEKVIKKRDGRMNAWLLDKQFKEEVPTSLNYCYDVDICLERAKYNN-----REVLVETLRAEFCK--------ILKIHSFEKLHTHTLMPVSTALAAASVRTKP---------VEVLDRSGVPVALPMDLRQNFVRFCVRNSVQRMKRFNFGRVYSQTSA-NGHPHERWECCVDCIGPQCSSPSLEAELLLVACEMMIGSLPG--MKFTLKIGHAQLIEAQIRHLKLSDDVRAELLDALHLISVSDRPHSHKEKMDMLTPKIGAKAANIITKLLI------PVEDNFGAFKEKVACFRKKLKVDAARVLVDKAIRDLEEIVGTFKFCRTEAIEQISIVYDSQTCYRP-RTFGDGLLFQI---------QVEKPSTIANNKR------GRRQNVLAGGRYDSALLRERHP--------RDFV----------------YEIPLCISGFGVAMDVVSQIRDSINKSANIPKTPQNHCKVLICSMVQPDGSNLITQKFELAKKLWSMGIEADVF 1452

HSP 2 Score: 78.1814 bits (191), Expect = 5.510e-14
Identity = 60/184 (32.61%), Postives = 91/184 (49.46%), Query Frame = 1

Query: 1339 KRYDLMKLGVLAKELF-------FFGIPETEREETDMPSDLI-DFITSCNS--NIEELKLHPFLT--CENLSKNSRQNSQTVDSAQDLTPWH-LFEKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTM-KEVQLLSSLNHENIVRYFASWSEEMQKSSSETNN 1848
            +R DL +LG L   L        +  +P       +  ++L+ +FI  C    NI++L   PFL   C++ S+N              TP+        R+  +  +I  LG G FGDVV VRN  D+  YAIKR+ LNA   K  R + KE +  + LNH N+VRY+ +W+E++     ET++
Sbjct:  399 RRKDLFQLGTLLDGLILATRGSTYSRVPTPVEGNQNTGTNLLGNFIAKCQEAKNIDQLVEDPFLKEECQSESENI------------FTPFGGAMSPDGRMLADNVIIRVLGRGGFGDVVLVRNKMDSTDYAIKRIPLNAKSDKLNRKIAKEAKFFAKLNHPNMVRYYYAWAEDLIPIVEETSD 570

[Close]

Query: 2287 KYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGL----AMNFNNKVTESANQE------YVKDNGL---SNDV-ENGPVGTIMYICP-----EMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVEL----IKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSL---LYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPKFINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIV-TDYDSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHY----SIRASLHDMVSK-DVKNLDSDHFRSLTNLTDK---QKANIKSILLFNGSRLEDLEKHFKIFQNRIH---------EGYDVINSAFKELKSVEEKLK----HFTEDLCFCVNFRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVN----------VVVQYDIEMDG--II---WDLVSRLWAANISAEIY 4350
            + L IQME C+    TL++YID+   +     +WR   E++ GL+++H   MIHRD+KP NIFL SQ  VKIGD GL    AM+   K+   A ++       +  NG+    +DV +   +GT +Y+ P     E+++++ YT+K D+YS G+++FEM ++P     +R   L NLR  +I  P +    + +    L    ++ +L  +   RP+A +LL    +P    ++   R     V K     +   +L + F +       Y   +   L ++ +N+     + ++   LR                +  F   +  ++     A+   +    P           +LD  GV V LP +LR NF  F V N +   KR+  G +Y Q +    HP ER E   D +     SP+  AE+L +  ++   S        TL+I +  L  A + H      +RA L D +    V +    H   +  LT K   + ANI + LL        +E +F  F+ ++              +++ A ++L+ +    K       E +    + +    P  T   GL+FQI          V KP     N R         Q + +GG Y S L++ + P          FV                Y I   I  FGV + V       +N + N          V++   ++ DG  +I   ++L  +LW+  I A+++
Sbjct:  776 RILCIQMEYCD--RATLRQYIDENHCFNAPTEVWRIFSEVLCGLKYMHDMAMIHRDIKPLNIFLTSQNGVKIGDFGLATLEAMSSKGKIVGGAAEKSTSIEAMLSPNGVKSKGSDVHQTRDIGTQLYMAPELFVDELVHKAPYTSKIDIYSAGVVLFEMFYRPLPPSMDRVSTLNNLRD-DIKIPSDFGAGLAAPMAGLARRTVEKMLQRNPDERPTADDLLNDEDLPMHTKEDATFRNLCEKVIKKRDGRMNAWLLDKQFKEEVPTSLNYCYDVDICLERAKYNN-----REVLVETLRAEFCK--------ILKIHSFEKLHTHTLMPVSTALAAASVRTKP---------VEVLDRSGVPVALPMDLRQNFVRFCVRNSVQRMKRFNFGRVYSQTSA-NGHPHERWECCVDCIGPQCSSPSLEAELLLVACEMMIGSLPG--MKFTLKIGHAQLIEAQIRHLKLSDDVRAELLDALHLISVSDRPHSHKEKMDMLTPKIGAKAANIITKLLI------PVEDNFGAFKEKVACFRKKLKVDAARVLVDKAIRDLEEIVGTFKFCRTEAIEQISIVYDSQTCYRP-RTFGDGLLFQI---------QVEKPSTIANNKR------GRRQNVLAGGRYDSALLRERHP--------RDFV----------------YEIPLCISGFGVAMDVVSQIRDSINKSANIPKTPQNHCKVLICSMVQPDGSNLITQKFELAKKLWSMGIEADVF 1452

HSP 2 Score: 78.1814 bits (191), Expect = 5.510e-14
Identity = 60/184 (32.61%), Postives = 91/184 (49.46%), Query Frame = 1

Query: 1339 KRYDLMKLGVLAKELF-------FFGIPETEREETDMPSDLI-DFITSCNS--NIEELKLHPFLT--CENLSKNSRQNSQTVDSAQDLTPWH-LFEKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTM-KEVQLLSSLNHENIVRYFASWSEEMQKSSSETNN 1848
            +R DL +LG L   L        +  +P       +  ++L+ +FI  C    NI++L   PFL   C++ S+N              TP+        R+  +  +I  LG G FGDVV VRN  D+  YAIKR+ LNA   K  R + KE +  + LNH N+VRY+ +W+E++     ET++
Sbjct:  399 RRKDLFQLGTLLDGLILATRGSTYSRVPTPVEGNQNTGTNLLGNFIAKCQEAKNIDQLVEDPFLKEECQSESENI------------FTPFGGAMSPDGRMLADNVIIRVLGRGGFGDVVLVRNKMDSTDYAIKRIPLNAKSDKLNRKIAKEAKFFAKLNHPNMVRYYYAWAEDLIPIVEETSD 570

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Celegans
Match: gcn-2 (Eukaryotic translation initiation factor 2-alpha kinase gcn-2 [Source:UniProtKB/Swiss-Prot;Acc:D0Z5N4])

HSP 1 Score: 167.548 bits (423), Expect = 3.297e-41
Identity = 186/751 (24.77%), Postives = 311/751 (41.41%), Query Frame = 1

Query: 2287 KYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGL----AMNFNNKVTESANQE------YVKDNGL---SNDV-ENGPVGTIMYICP-----EMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVEL----IKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSL---LYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPKFINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIV-TDYDSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHY----SIRASLHDMVSK-DVKNLDSDHFRSLTNLTDK---QKANIKSILLFNGSRLEDLEKHFKIFQNRIH---------EGYDVINSAFKELKSVEEKLK----HFTEDLCFCVNFRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVN----------VVVQYDIEMDG--II---WDLVSRLWAANISAEIY 4350
            + L IQME C+    TL++YID+   +     +WR   E++ GL+++H   MIHRD+KP NIFL SQ  VKIGD GL    AM+   K+   A ++       +  NG+    +DV +   +GT +Y+ P     E+++++ YT+K D+YS G+++FEM ++P     +R   L NLR  +I  P +    + +    L    ++ +L  +   RP+A +LL    +P    ++   R     V K     +   +L + F +       Y   +   L ++ +N+     + ++   LR                +  F   +  ++     A+   +    P           +LD  GV V LP +LR NF  F V N +   KR+  G +Y Q +    HP ER E   D +     SP+  AE+L +  ++   S        TL+I +  L  A + H      +RA L D +    V +    H   +  LT K   + ANI + LL        +E +F  F+ ++              +++ A ++L+ +    K       E +    + +    P  T   GL+FQI          V KP     N R         Q + +GG Y S L++ + P          FV                Y I   I  FGV + V       +N + N          V++   ++ DG  +I   ++L  +LW+  I A+++
Sbjct:  776 RILCIQMEYCD--RATLRQYIDENHCFNAPTEVWRIFSEVLCGLKYMHDMAMIHRDIKPLNIFLTSQNGVKIGDFGLATLEAMSSKGKIVGGAAEKSTSIEAMLSPNGVKSKGSDVHQTRDIGTQLYMAPELFVDELVHKAPYTSKIDIYSAGVVLFEMFYRPLPPSMDRVSTLNNLRD-DIKIPSDFGAGLAAPMAGLARRTVEKMLQRNPDERPTADDLLNDEDLPMHTKEDATFRNLCEKVIKKRDGRMNAWLLDKQFKEEVPTSLNYCYDVDICLERAKYNN-----REVLVETLRAEFCK--------ILKIHSFEKLHTHTLMPVSTALAAASVRTKP---------VEVLDRSGVPVALPMDLRQNFVRFCVRNSVQRMKRFNFGRVYSQTSA-NGHPHERWECCVDCIGPQCSSPSLEAELLLVACEMMIGSLPG--MKFTLKIGHAQLIEAQIRHLKLSDDVRAELLDALHLISVSDRPHSHKEKMDMLTPKIGAKAANIITKLLI------PVEDNFGAFKEKVACFRKKLKVDAARVLVDKAIRDLEEIVGTFKFCRTEAIEQISIVYDSQTCYRP-RTFGDGLLFQI---------QVEKPSTIANNKR------GRRQNVLAGGRYDSALLRERHP--------RDFV----------------YEIPLCISGFGVAMDVVSQIRDSINKSANIPKTPQNHCKVLICSMVQPDGSNLITQKFELAKKLWSMGIEADVF 1452

HSP 2 Score: 78.1814 bits (191), Expect = 5.370e-14
Identity = 60/184 (32.61%), Postives = 91/184 (49.46%), Query Frame = 1

Query: 1339 KRYDLMKLGVLAKELF-------FFGIPETEREETDMPSDLI-DFITSCNS--NIEELKLHPFLT--CENLSKNSRQNSQTVDSAQDLTPWH-LFEKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTM-KEVQLLSSLNHENIVRYFASWSEEMQKSSSETNN 1848
            +R DL +LG L   L        +  +P       +  ++L+ +FI  C    NI++L   PFL   C++ S+N              TP+        R+  +  +I  LG G FGDVV VRN  D+  YAIKR+ LNA   K  R + KE +  + LNH N+VRY+ +W+E++     ET++
Sbjct:  399 RRKDLFQLGTLLDGLILATRGSTYSRVPTPVEGNQNTGTNLLGNFIAKCQEAKNIDQLVEDPFLKEECQSESENI------------FTPFGGAMSPDGRMLADNVIIRVLGRGGFGDVVLVRNKMDSTDYAIKRIPLNAKSDKLNRKIAKEAKFFAKLNHPNMVRYYYAWAEDLIPIVEETSD 570

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Celegans
Match: gcn-2 (Eukaryotic translation initiation factor 2-alpha kinase gcn-2 [Source:UniProtKB/Swiss-Prot;Acc:D0Z5N4])

HSP 1 Score: 167.548 bits (423), Expect = 3.297e-41
Identity = 186/751 (24.77%), Postives = 311/751 (41.41%), Query Frame = 1

Query: 2287 KYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGL----AMNFNNKVTESANQE------YVKDNGL---SNDV-ENGPVGTIMYICP-----EMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVEL----IKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSL---LYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPKFINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIV-TDYDSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHY----SIRASLHDMVSK-DVKNLDSDHFRSLTNLTDK---QKANIKSILLFNGSRLEDLEKHFKIFQNRIH---------EGYDVINSAFKELKSVEEKLK----HFTEDLCFCVNFRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVN----------VVVQYDIEMDG--II---WDLVSRLWAANISAEIY 4350
            + L IQME C+    TL++YID+   +     +WR   E++ GL+++H   MIHRD+KP NIFL SQ  VKIGD GL    AM+   K+   A ++       +  NG+    +DV +   +GT +Y+ P     E+++++ YT+K D+YS G+++FEM ++P     +R   L NLR  +I  P +    + +    L    ++ +L  +   RP+A +LL    +P    ++   R     V K     +   +L + F +       Y   +   L ++ +N+     + ++   LR                +  F   +  ++     A+   +    P           +LD  GV V LP +LR NF  F V N +   KR+  G +Y Q +    HP ER E   D +     SP+  AE+L +  ++   S        TL+I +  L  A + H      +RA L D +    V +    H   +  LT K   + ANI + LL        +E +F  F+ ++              +++ A ++L+ +    K       E +    + +    P  T   GL+FQI          V KP     N R         Q + +GG Y S L++ + P          FV                Y I   I  FGV + V       +N + N          V++   ++ DG  +I   ++L  +LW+  I A+++
Sbjct:  776 RILCIQMEYCD--RATLRQYIDENHCFNAPTEVWRIFSEVLCGLKYMHDMAMIHRDIKPLNIFLTSQNGVKIGDFGLATLEAMSSKGKIVGGAAEKSTSIEAMLSPNGVKSKGSDVHQTRDIGTQLYMAPELFVDELVHKAPYTSKIDIYSAGVVLFEMFYRPLPPSMDRVSTLNNLRD-DIKIPSDFGAGLAAPMAGLARRTVEKMLQRNPDERPTADDLLNDEDLPMHTKEDATFRNLCEKVIKKRDGRMNAWLLDKQFKEEVPTSLNYCYDVDICLERAKYNN-----REVLVETLRAEFCK--------ILKIHSFEKLHTHTLMPVSTALAAASVRTKP---------VEVLDRSGVPVALPMDLRQNFVRFCVRNSVQRMKRFNFGRVYSQTSA-NGHPHERWECCVDCIGPQCSSPSLEAELLLVACEMMIGSLPG--MKFTLKIGHAQLIEAQIRHLKLSDDVRAELLDALHLISVSDRPHSHKEKMDMLTPKIGAKAANIITKLLI------PVEDNFGAFKEKVACFRKKLKVDAARVLVDKAIRDLEEIVGTFKFCRTEAIEQISIVYDSQTCYRP-RTFGDGLLFQI---------QVEKPSTIANNKR------GRRQNVLAGGRYDSALLRERHP--------RDFV----------------YEIPLCISGFGVAMDVVSQIRDSINKSANIPKTPQNHCKVLICSMVQPDGSNLITQKFELAKKLWSMGIEADVF 1452

HSP 2 Score: 78.1814 bits (191), Expect = 5.370e-14
Identity = 60/184 (32.61%), Postives = 91/184 (49.46%), Query Frame = 1

Query: 1339 KRYDLMKLGVLAKELF-------FFGIPETEREETDMPSDLI-DFITSCNS--NIEELKLHPFLT--CENLSKNSRQNSQTVDSAQDLTPWH-LFEKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTM-KEVQLLSSLNHENIVRYFASWSEEMQKSSSETNN 1848
            +R DL +LG L   L        +  +P       +  ++L+ +FI  C    NI++L   PFL   C++ S+N              TP+        R+  +  +I  LG G FGDVV VRN  D+  YAIKR+ LNA   K  R + KE +  + LNH N+VRY+ +W+E++     ET++
Sbjct:  399 RRKDLFQLGTLLDGLILATRGSTYSRVPTPVEGNQNTGTNLLGNFIAKCQEAKNIDQLVEDPFLKEECQSESENI------------FTPFGGAMSPDGRMLADNVIIRVLGRGGFGDVVLVRNKMDSTDYAIKRIPLNAKSDKLNRKIAKEAKFFAKLNHPNMVRYYYAWAEDLIPIVEETSD 570

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Fly
Match: Gcn2 (gene:FBgn0019990 transcript:FBtr0334552)

HSP 1 Score: 215.698 bits (548), Expect = 7.024e-56
Identity = 168/564 (29.79%), Postives = 273/564 (48.40%), Query Frame = 1

Query: 2233 YINDSQNNPFHGNKTKIEKYLM----IQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMN--FNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSS---YTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDE--KHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPKFINTES-----GFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDYDSPNKT-AEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSI-RASLHDMVSKDVKNLDSDHFR-----SLTNLTDKQKANIKSIL-------LFNGSRLEDLEKHFKIFQNRIHEGYDVINSAFKELKSV 3834
            Y ++ Q +   G KT   K LM    IQME CE    TL+  IDD  L+ +   +WR  REI  GL HIH + +IHRDLKP NIFLDS  ++KIGD GLA       +  ++A     +     +    G VGT +Y+ PE+   +S   Y  K DMY+LGII+FEMC  PF T  ER   +  LR   I  PD   K  K + + V++++ LLNHD   RP+A ELL S+++PP + +  EL++ L     N  S  YK ++     +   E+  +           + + +++++   N     S+ I +       +  FV   + ++F  HGA+E  +PLLSP      +         L+   G VV LP +LR  FA  +  N ++  +RY +  +Y+++ +   HP++  + +FDI+      +   AE+L L  +I  +        L + +N+TNL  A++   ++ +A    +    +  ++S   R     S+T + +K + + ++++       L  GSR    +   K       E   +   A +EL++V
Sbjct:  673 YDDEEQEDSTRG-KTNSPKPLMQVMYIQMEFCE--KCTLRTAIDD-NLFNDTDRLWRLFREIAEGLAHIHQQGIIHRDLKPVNIFLDSHDQIKIGDFGLATTSFLALQAHDAAPAPVNQITSAEDGTGTGKVGTTLYVAPELTGNASKSVYNQKVDMYTLGIILFEMCQPPFDTSMERAQTIMALRNVSINIPDAMLKDPKYEKT-VKMLQWLLNHDPAQRPTAEELLISDLVPPAQLEANELQEMLRHALANPQSKAYKNLVARCLQQESDEVLEH----------TYHLGSSRAMKSWN-----SAIIIDDIVSLNPVIEFVKAKVVNLFRKHGAIEVDSPLLSPLSARNSTANANANAVHLMTHSGCVVVLPCDLRTQFARHVTMNSVNLIRRYCVDRVYREERVFNFHPKQSYDCSFDIIAPTTGSHLVDAELLSLAFEITSELPRLREKNLAIRMNHTNLLRAILIFCNVPKAQYGALFEGTMDFIESRISRFQFHSSITGIMEKSRTSAQTLMDMLLANFLLTGSRSTVDDSALKSLMRGKGEAASLARGALRELETV 1216

HSP 2 Score: 150.214 bits (378), Expect = 8.598e-36
Identity = 156/642 (24.30%), Postives = 284/642 (44.24%), Query Frame = 1

Query:  124 QSDEVKALRSIFGKEFKLIRPKNLKKKYEPPLVSVLINV--DSSN-LNSALSLRISFDCPQNYPFDIPKISIYDAVNVPTNLVEQLLIILNEKSNLLKGYSMIYNLVEEARCIIIKHFETDHKSVNSILTINK---------VLNEDEIMQTET------KQSEFLKNLLRQRRDSARXXXXXXXXXXXC------------DHSYKTDELEFEHCSD---RSSGCLVYKGICDGQHRIKTINPNMQIKLNTFNGFNRQKGTNVKI--YQIRFSDLKRKQFEDLKSNW---------------LSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCE--MDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFC-IGKRFEELKGRGHSNERTGASTIKKGSKRYDLMKLGVLAKELFFFGIPETEREETDMPS---DLIDFITSCNSN----IEELKLHPFL-------TCENLSKNSRQNSQTVDSAQDLTPWH---LFEKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTM-KEVQLLSSLNHENIVRYFASWSEEMQKSSS 1836
            Q+ E++ ++SIFG + + +RP+     ++P  + + +    DSSN L + +  ++   CP  YP   PKIS+ ++  +   L+E L   L  +S  L+G  MIY L +  +  +++H +    S    +  +K         +  + E +Q +T      ++ E  K   ++R +  R+  ++  +                 H Y +  L+  +         G  + +G C G+        + Q     + G +   G  + I  + I++S L++      K +W               ++ +E+  + L +L H +L++Y        E V+                 EGL ++ V +  + T   S  ++L   +  ++++   + +L+A+++LH+K ++   +   ++ +D    +++ +F  +    E L G G S            S   DL  LG L + L              MP+   ++ DF+  CNS+      EL  HPFL         + +    +Q+  TV       P+    L    SR+  EFE++  LG+GAFGDV+KVRNI DN+ YAIKR+ L A  ++  + M +EV+LLS LNHEN+VRYF SW E +  + +
Sbjct:   14 QAQELEVIKSIFGCDVEDLRPQANPSLWKPTDIRIQLTPLRDSSNGLETYVCTKLHVTCPSKYPKLPPKISLEESKGMSDQLLEALRNQLQAQSQELRGEVMIYELAQTVQAFLLEHNKPPKGSFYDQMLQDKQKRDQELQDIQRQRESLQRQTLIDEVERRKEMFKTEAKRRGEPRRSMSESNPRHPSSSESSENSSPYYRGHIYPSKCLDHRNTETLYFHKMGRQIQRGCCVGEG-------HSQRGCIAYTGIDMHCGQLLYITEWNIKYSQLEQPCIGGGKCHWSSESKCMGSHRVDEVMASIEKQVSSLSQLQHKNLVSY--------ECVL------------CIKRKEGLLVYLVQDFLLGTSVFSISSSLGWCMDGARMVA--RGVLDALVFLHNKGVSHSHLLDTTVFMDNTGNVRVSDFSLVPNLLELLSGAGQS------------SSCGDLPALGALVESL--------------MPTNSYEMRDFVDKCNSDRTLSASELLEHPFLRFYVDNGQQQVMPLPQQQHPNTVQRTGSAMPYQIPTLALSQSRLRTEFEVLMYLGKGAFGDVLKVRNILDNREYAIKRIPLPARSRQLYKKMTREVELLSRLNHENVVRYFNSWIESVDDADA 600

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Fly
Match: Gcn2 (gene:FBgn0019990 transcript:FBtr0334551)

HSP 1 Score: 215.698 bits (548), Expect = 7.130e-56
Identity = 168/564 (29.79%), Postives = 273/564 (48.40%), Query Frame = 1

Query: 2233 YINDSQNNPFHGNKTKIEKYLM----IQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMN--FNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSS---YTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDE--KHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPKFINTES-----GFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDYDSPNKT-AEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSI-RASLHDMVSKDVKNLDSDHFR-----SLTNLTDKQKANIKSIL-------LFNGSRLEDLEKHFKIFQNRIHEGYDVINSAFKELKSV 3834
            Y ++ Q +   G KT   K LM    IQME CE    TL+  IDD  L+ +   +WR  REI  GL HIH + +IHRDLKP NIFLDS  ++KIGD GLA       +  ++A     +     +    G VGT +Y+ PE+   +S   Y  K DMY+LGII+FEMC  PF T  ER   +  LR   I  PD   K  K + + V++++ LLNHD   RP+A ELL S+++PP + +  EL++ L     N  S  YK ++     +   E+  +           + + +++++   N     S+ I +       +  FV   + ++F  HGA+E  +PLLSP      +         L+   G VV LP +LR  FA  +  N ++  +RY +  +Y+++ +   HP++  + +FDI+      +   AE+L L  +I  +        L + +N+TNL  A++   ++ +A    +    +  ++S   R     S+T + +K + + ++++       L  GSR    +   K       E   +   A +EL++V
Sbjct:  671 YDDEEQEDSTRG-KTNSPKPLMQVMYIQMEFCEK--CTLRTAIDD-NLFNDTDRLWRLFREIAEGLAHIHQQGIIHRDLKPVNIFLDSHDQIKIGDFGLATTSFLALQAHDAAPAPVNQITSAEDGTGTGKVGTTLYVAPELTGNASKSVYNQKVDMYTLGIILFEMCQPPFDTSMERAQTIMALRNVSINIPDAMLKDPKYEKT-VKMLQWLLNHDPAQRPTAEELLISDLVPPAQLEANELQEMLRHALANPQSKAYKNLVARCLQQESDEVLEH----------TYHLGSSRAMKSWN-----SAIIIDDIVSLNPVIEFVKAKVVNLFRKHGAIEVDSPLLSPLSARNSTANANANAVHLMTHSGCVVVLPCDLRTQFARHVTMNSVNLIRRYCVDRVYREERVFNFHPKQSYDCSFDIIAPTTGSHLVDAELLSLAFEITSELPRLREKNLAIRMNHTNLLRAILIFCNVPKAQYGALFEGTMDFIESRISRFQFHSSITGIMEKSRTSAQTLMDMLLANFLLTGSRSTVDDSALKSLMRGKGEAASLARGALRELETV 1214

HSP 2 Score: 150.984 bits (380), Expect = 4.710e-36
Identity = 156/642 (24.30%), Postives = 280/642 (43.61%), Query Frame = 1

Query:  124 QSDEVKALRSIFGKEFKLIRPKNLKKKYEPPLVSVLINV--DSSN-LNSALSLRISFDCPQNYPFDIPKISIYDAVNVPTNLVEQLLIILNEKSNLLKGYSMIYNLVEEARCIIIKH-------------------------FETDHKSVNSILTINKVLNEDEIMQTETKQSEFLKNLLRQR--RDSARXXXXXXXXXXXCDHSYKTDELEFEHCSD---RSSGCLVYKGICDGQHRIKTINPNMQIKLNTFNGFNRQKGTNVKI--YQIRFSDLKRKQFEDLKSNW---------------LSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCE--MDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFC-IGKRFEELKGRGHSNERTGASTIKKGSKRYDLMKLGVLAKELFFFGIPETEREETDMPS---DLIDFITSCNSN----IEELKLHPFL-------TCENLSKNSRQNSQTVDSAQDLTPWH---LFEKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTM-KEVQLLSSLNHENIVRYFASWSEEMQKSSS 1836
            Q+ E++ ++SIFG + + +RP+     ++P  + + +    DSSN L + +  ++   CP  YP   PKIS+ ++  +   L+E L   L  +S  L+G  MIY L +  +  +++H                          +   +S+     I++V    E+ +TE K+    +  + +   R  + +E           H Y +  L+  +         G  + +G C G  +   I          + G +   G  + I  + I++S L++      K +W               ++ +E+  + L +L H +L++Y        E V+                 EGL ++ V +  + T   S  ++L   +  ++++   + +L+A+++LH+K ++   +   ++ +D    +++ +F  +    E L G G S            S   DL  LG L + L              MP+   ++ DF+  CNS+      EL  HPFL         + +    +Q+  TV       P+    L    SR+  EFE++  LG+GAFGDV+KVRNI DN+ YAIKR+ L A  ++  + M +EV+LLS LNHEN+VRYF SW E +  + +
Sbjct:   14 QAQELEVIKSIFGCDVEDLRPQANPSLWKPTDIRIQLTPLRDSSNGLETYVCTKLHVTCPSKYPKLPPKISLEESKGMSDQLLEALRNQLQAQSQELRGEVMIYELAQTVQAFLLEHNKPPKGSFYDQMLQDKQKRDQELQDIQRQRESLQRQTLIDEVERRKEMFKTEAKRRGEPRRSMSESNPRHPSSSESSENSSPYYRGHIYPSKCLDHRNTETLYFHKMGRQIQRGCCVGHSQRGCI---------AYTGIDMHCGQLLYITEWNIKYSQLEQPCIGGGKCHWSSESKCMGSHRVDEVMASIEKQVSSLSQLQHKNLVSY--------ECVL------------CIKRKEGLLVYLVQDFLLGTSVFSISSSLGWCMDGARMVA--RGVLDALVFLHNKGVSHSHLLDTTVFMDNTGNVRVSDFSLVPNLLELLSGAGQS------------SSCGDLPALGALVESL--------------MPTNSYEMRDFVDKCNSDRTLSASELLEHPFLRFYVDNGQQQVMPLPQQQHPNTVQRTGSAMPYQIPTLALSQSRLRTEFEVLMYLGKGAFGDVLKVRNILDNREYAIKRIPLPARSRQLYKKMTREVELLSRLNHENVVRYFNSWIESVDDADA 598

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Fly
Match: Gcn2 (gene:FBgn0019990 transcript:FBtr0085787)

HSP 1 Score: 215.698 bits (548), Expect = 7.130e-56
Identity = 168/564 (29.79%), Postives = 273/564 (48.40%), Query Frame = 1

Query: 2233 YINDSQNNPFHGNKTKIEKYLM----IQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMN--FNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSS---YTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDE--KHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPKFINTES-----GFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDYDSPNKT-AEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSI-RASLHDMVSKDVKNLDSDHFR-----SLTNLTDKQKANIKSIL-------LFNGSRLEDLEKHFKIFQNRIHEGYDVINSAFKELKSV 3834
            Y ++ Q +   G KT   K LM    IQME CE    TL+  IDD  L+ +   +WR  REI  GL HIH + +IHRDLKP NIFLDS  ++KIGD GLA       +  ++A     +     +    G VGT +Y+ PE+   +S   Y  K DMY+LGII+FEMC  PF T  ER   +  LR   I  PD   K  K + + V++++ LLNHD   RP+A ELL S+++PP + +  EL++ L     N  S  YK ++     +   E+  +           + + +++++   N     S+ I +       +  FV   + ++F  HGA+E  +PLLSP      +         L+   G VV LP +LR  FA  +  N ++  +RY +  +Y+++ +   HP++  + +FDI+      +   AE+L L  +I  +        L + +N+TNL  A++   ++ +A    +    +  ++S   R     S+T + +K + + ++++       L  GSR    +   K       E   +   A +EL++V
Sbjct:  671 YDDEEQEDSTRG-KTNSPKPLMQVMYIQMEFCEK--CTLRTAIDD-NLFNDTDRLWRLFREIAEGLAHIHQQGIIHRDLKPVNIFLDSHDQIKIGDFGLATTSFLALQAHDAAPAPVNQITSAEDGTGTGKVGTTLYVAPELTGNASKSVYNQKVDMYTLGIILFEMCQPPFDTSMERAQTIMALRNVSINIPDAMLKDPKYEKT-VKMLQWLLNHDPAQRPTAEELLISDLVPPAQLEANELQEMLRHALANPQSKAYKNLVARCLQQESDEVLEH----------TYHLGSSRAMKSWN-----SAIIIDDIVSLNPVIEFVKAKVVNLFRKHGAIEVDSPLLSPLSARNSTANANANAVHLMTHSGCVVVLPCDLRTQFARHVTMNSVNLIRRYCVDRVYREERVFNFHPKQSYDCSFDIIAPTTGSHLVDAELLSLAFEITSELPRLREKNLAIRMNHTNLLRAILIFCNVPKAQYGALFEGTMDFIESRISRFQFHSSITGIMEKSRTSAQTLMDMLLANFLLTGSRSTVDDSALKSLMRGKGEAASLARGALRELETV 1214

HSP 2 Score: 150.984 bits (380), Expect = 4.710e-36
Identity = 156/642 (24.30%), Postives = 280/642 (43.61%), Query Frame = 1

Query:  124 QSDEVKALRSIFGKEFKLIRPKNLKKKYEPPLVSVLINV--DSSN-LNSALSLRISFDCPQNYPFDIPKISIYDAVNVPTNLVEQLLIILNEKSNLLKGYSMIYNLVEEARCIIIKH-------------------------FETDHKSVNSILTINKVLNEDEIMQTETKQSEFLKNLLRQR--RDSARXXXXXXXXXXXCDHSYKTDELEFEHCSD---RSSGCLVYKGICDGQHRIKTINPNMQIKLNTFNGFNRQKGTNVKI--YQIRFSDLKRKQFEDLKSNW---------------LSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCE--MDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFC-IGKRFEELKGRGHSNERTGASTIKKGSKRYDLMKLGVLAKELFFFGIPETEREETDMPS---DLIDFITSCNSN----IEELKLHPFL-------TCENLSKNSRQNSQTVDSAQDLTPWH---LFEKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTM-KEVQLLSSLNHENIVRYFASWSEEMQKSSS 1836
            Q+ E++ ++SIFG + + +RP+     ++P  + + +    DSSN L + +  ++   CP  YP   PKIS+ ++  +   L+E L   L  +S  L+G  MIY L +  +  +++H                          +   +S+     I++V    E+ +TE K+    +  + +   R  + +E           H Y +  L+  +         G  + +G C G  +   I          + G +   G  + I  + I++S L++      K +W               ++ +E+  + L +L H +L++Y        E V+                 EGL ++ V +  + T   S  ++L   +  ++++   + +L+A+++LH+K ++   +   ++ +D    +++ +F  +    E L G G S            S   DL  LG L + L              MP+   ++ DF+  CNS+      EL  HPFL         + +    +Q+  TV       P+    L    SR+  EFE++  LG+GAFGDV+KVRNI DN+ YAIKR+ L A  ++  + M +EV+LLS LNHEN+VRYF SW E +  + +
Sbjct:   14 QAQELEVIKSIFGCDVEDLRPQANPSLWKPTDIRIQLTPLRDSSNGLETYVCTKLHVTCPSKYPKLPPKISLEESKGMSDQLLEALRNQLQAQSQELRGEVMIYELAQTVQAFLLEHNKPPKGSFYDQMLQDKQKRDQELQDIQRQRESLQRQTLIDEVERRKEMFKTEAKRRGEPRRSMSESNPRHPSSSESSENSSPYYRGHIYPSKCLDHRNTETLYFHKMGRQIQRGCCVGHSQRGCI---------AYTGIDMHCGQLLYITEWNIKYSQLEQPCIGGGKCHWSSESKCMGSHRVDEVMASIEKQVSSLSQLQHKNLVSY--------ECVL------------CIKRKEGLLVYLVQDFLLGTSVFSISSSLGWCMDGARMVA--RGVLDALVFLHNKGVSHSHLLDTTVFMDNTGNVRVSDFSLVPNLLELLSGAGQS------------SSCGDLPALGALVESL--------------MPTNSYEMRDFVDKCNSDRTLSASELLEHPFLRFYVDNGQQQVMPLPQQQHPNTVQRTGSAMPYQIPTLALSQSRLRTEFEVLMYLGKGAFGDVLKVRNILDNREYAIKRIPLPARSRQLYKKMTREVELLSRLNHENVVRYFNSWIESVDDADA 598

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Fly
Match: PEK (gene:FBgn0037327 transcript:FBtr0078770)

HSP 1 Score: 97.8265 bits (242), Expect = 5.834e-20
Identity = 73/224 (32.59%), Postives = 111/224 (49.55%), Query Frame = 1

Query: 2239 NDSQNNP-----FHGNKTKIEK-YLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFN---NKVTESANQEYVKDNGLSNDVENG-PVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASEL 2880
            N SQ  P      +G   K  K YL IQM+LC  +  +L+ ++ D +       I     +IV  ++++H K +IHRDLKP NIF     ++KIGD GL  +     N V +  +Q     +GL +   +   VGT +Y+ PE L    Y  K D+YSLG+I FE+ H  F T  ER   L +LR  +  +P +        + +L++ +L+     RP   +L
Sbjct:  905 NGSQPTPSSATILNGTVAKPSKVYLYIQMQLCRKE--SLRDWLRDNRSETRAAHIGDIFHQIVDAVDYVHLKGLIHRDLKPSNIFFSQDGQIKIGDFGLVTDMADIPNLVAKCGDQ-----SGLPSCARHTQQVGTHLYMSPEQLLGQHYDYKVDIYSLGLIFFEL-HVYFSTEMERIKTLRSLRDGQ--YPKDFAVNYPQQY-DLLQQMLSAQPEQRPQTKQL 1117

HSP 2 Score: 75.485 bits (184), Expect = 3.890e-13
Identity = 47/148 (31.76%), Postives = 72/148 (48.65%), Query Frame = 1

Query: 1591 SSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSSAISVIKPE--TTDSLLLDFQDKKNSIMFSANVNFSQIEHCSRHFDL 2028
            +SR   +FEL+  LG G FG V + +N  D   YAIKR+ L   +    R ++E + L+S  H NIVRYF SW+E      +     Q   D   + +  S++I +  P+  T  SL    ++K+   + S   + +    CS  F L
Sbjct:  635 TSRFQSDFELMQCLGRGGFGVVFEAKNKLDENRYAIKRITLPNKESSRQRVLREARTLASCEHHNIVRYFHSWTE------TPPTGWQEEEDRKLLAHELSTSIQIETPDDSTMPSLTEQLKEKRQQQLLSWVSDAANSTACSHDFHL 776

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Fly
Match: PEK (gene:FBgn0037327 transcript:FBtr0330026)

HSP 1 Score: 97.8265 bits (242), Expect = 7.014e-20
Identity = 73/224 (32.59%), Postives = 111/224 (49.55%), Query Frame = 1

Query: 2239 NDSQNNP-----FHGNKTKIEK-YLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFN---NKVTESANQEYVKDNGLSNDVENG-PVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASEL 2880
            N SQ  P      +G   K  K YL IQM+LC  +  +L+ ++ D +       I     +IV  ++++H K +IHRDLKP NIF     ++KIGD GL  +     N V +  +Q     +GL +   +   VGT +Y+ PE L    Y  K D+YSLG+I FE+ H  F T  ER   L +LR  +  +P +        + +L++ +L+     RP   +L
Sbjct:  853 NGSQPTPSSATILNGTVAKPSKVYLYIQMQLCRKE--SLRDWLRDNRSETRAAHIGDIFHQIVDAVDYVHLKGLIHRDLKPSNIFFSQDGQIKIGDFGLVTDMADIPNLVAKCGDQ-----SGLPSCARHTQQVGTHLYMSPEQLLGQHYDYKVDIYSLGLIFFEL-HVYFSTEMERIKTLRSLRDGQ--YPKDFAVNYPQQY-DLLQQMLSAQPEQRPQTKQL 1065

HSP 2 Score: 75.485 bits (184), Expect = 4.449e-13
Identity = 47/148 (31.76%), Postives = 72/148 (48.65%), Query Frame = 1

Query: 1591 SSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSSAISVIKPE--TTDSLLLDFQDKKNSIMFSANVNFSQIEHCSRHFDL 2028
            +SR   +FEL+  LG G FG V + +N  D   YAIKR+ L   +    R ++E + L+S  H NIVRYF SW+E      +     Q   D   + +  S++I +  P+  T  SL    ++K+   + S   + +    CS  F L
Sbjct:  583 TSRFQSDFELMQCLGRGGFGVVFEAKNKLDENRYAIKRITLPNKESSRQRVLREARTLASCEHHNIVRYFHSWTE------TPPTGWQEEEDRKLLAHELSTSIQIETPDDSTMPSLTEQLKEKRQQQLLSWVSDAANSTACSHDFHL 724

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Zebrafish
Match: EIF2AK4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:HGNC Symbol;Acc:HGNC:19687])

HSP 1 Score: 351.673 bits (901), Expect = 4.331e-99
Identity = 296/1037 (28.54%), Postives = 482/1037 (46.48%), Query Frame = 1

Query:  667 EFEHCSDRSSG-CLVYKGICDGQHRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQ--IRFSDLKRKQFED-------LKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKRF-----EELKGRGHSNERTGASTIKKGSKRYDLMKLGV------LAKEL--FFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFLTCENL-----SKNSRQNSQTVDSAQDLTPWHLF----------EKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSS-SETNNLQPNIDSSSIRNNTSSAISVIKPETTDSLLLDFQDKKNSIMFSANVNFSQIEHCSRHFDLESFXXXXXXXXXXXXVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPFHGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGL-----SNDVENGPVGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFT--LKRFVTRYIESIFVSHGAVEFLAPLLSPK----FINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDYDSP-NKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNH 3597
            E  H S+ S G  +V +G C G+        + ++  + +  FN   G    +Y+  +R++    K F              +  +E +   L KL HPHL+ Y        E  +  T H  L+ ++V+           C +  +  SG       + + ++  + + +L A+ YLH  ++    ++   +L+D    +++ ++ + KR      E++  + H      A   + G K+ D+   G+      + +E+  +   +P+T      +P+DL DF+  C         +  +L  HPFL   +       + S      VD A  + P  L           ++ SR   EF  +  LG+GAFG V+KV+N  D  YYA+KR+++N   K+  R   EV LLS LNHENIVRY+ +W E  + SS +E  +      S+  R +  +A S  +     SLL   +++      +++V +S     S      +    D + + +  V      P       +I F   DN D          + D L +Q  S R  +  ++ + +         I  YL IQME CE   +TL+  ID Q L+ +   +WR  REI+ GL +IH + MIHRDLKP NIFLDSQ  VKIGD GLA +    V     +     +G        D   G VGT +Y+ PE+    +++Y  K D++SLGII+FEM ++P  T SER  +L  LR+  I FP++       +  ++I  LLNHD  LRP+A ELL+S+++PPP+ +E EL + L     N +   Y+ M+ +LFS+  S +  Y                              + I +G F   +  L+++V   +  IF  HGAV    PLL P+    +   E+  F  +D  G++V+LP +LR+ FA F+  N IS  KRY+I  +++ + + R HPRE +E AFDI+    S     AE +Y + ++ ++    +    T+ +N+T+L  A++ H
Sbjct:  151 EVLHFSNSSLGEVVVQRGKCIGE--------SERLGRSVYYAFNSISGDFAVVYEWILRWNKTIGKFFTSQEKEKIEKCKKQIQGVESELNSLLKLQHPHLVHYQAMSISESEDSL--TVH--LLAEHVSG----------CSLAERVSSG-----CAVPVDELRRHTQQLLSALHYLHTNSVVHKHLSASCVLLDARGDVRLSDYSLCKRLSDVCKEDIFEQSHVRFSEDALPARAG-KKGDVWSFGLLLLALAVGREVKEYPLTVPDT------LPADLRDFLNRCVCLDDADRWHTHQLLDHPFLRPPSPKSLPPCQESSPEDTGVDFASTVVPRSLLLSAPFSTEVQKQFSRYYNEFSELQLLGKGAFGAVIKVQNKLDGCYYAVKRIQVNPASKQFRRIKGEVTLLSRLNHENIVRYYNAWIERQEMSSGAELTSDSSEALSTPERPSRPAAPS--RRNEDLSLLGRLEEEAPPPALASSVEWSTSLERSSSAKCSTADSEDEEEEEEEDVFCPSFLPPDSDSESDIIF---DNADA---------SRDSLLQQEPSSRKAADPLDSTDSE----KPLLIAHYLYIQMEYCEK--STLRDTID-QGLFEDSSRLWRLFREILDGLSYIHEQGMIHRDLKPVNIFLDSQDHVKIGDFGLATDHPANVAAGKLEVDESSSGFVPKPDPTDNMTGMVGTALYVGPEVQGNTKATYNQKVDLFSLGIILFEMSYRPMSTASERIFVLSQLRKESITFPEDFCKYESGTQRKVIGWLLNHDPALRPTAVELLKSDLLPPPQMEESELHEVLQHTMANVNGKAYRTMVNQLFSQNISPVTDYT---------------------------YDTDIHKGSFHLASAKLQQYVYETVTRIFRRHGAVRLQTPLLLPRNRRLYEGCETACF--MDHSGMLVSLPYDLRLAFARFVARNNISHLKRYSIERVFRPRKLDRVHPRELLECAFDIIIPISSSLMPDAEAIYTVSEVIQEFSALQDRNYTIHLNHTSLLKAILLH 1103

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Zebrafish
Match: eif2ak1 (eukaryotic translation initiation factor 2-alpha kinase 1 [Source:ZFIN;Acc:ZDB-GENE-061013-268])

HSP 1 Score: 179.104 bits (453), Expect = 2.211e-46
Identity = 155/500 (31.00%), Postives = 250/500 (50.00%), Query Frame = 1

Query: 1489 HPFLTCENLSKNSRQNSQTVDSAQDLTPWHLFE-KSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKK-AARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSSAISVIKPETTDSLLLDFQDKKNS--IMFSANVNFSQIEHCSRHFDLESFXXXXXXXXXXXXVELKDQSPRYKQ---PSVEISFGYSDNDDNPLDNHHKKN-TPDELSKQVKSMRLKSGYINDSQNNPFHGNKTKIEKYLM--IQMELCEGQYNTLQKYIDDQKLYMNEKLI--------------WRFIREIVSGLEHIHSKNMIHRDLKPHNIFL-DSQKRVKIGDLGLA-----MNFNNKVTESANQEYVKDNGLSNDV-ENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNI 2895
            H +L+ E  SK ++                LF+ ++SR   EFE I  LG+G++G V +V N  D Q YA+K++ +  + +    + ++EV++LSSL H NIV Y  +W E +Q + +        +DS+S+ + T  A+   +P   D    +  +  N   I+F  +    Q E       LES S   S S  D +  +  ++ R  Q   P V +    S  +  P  N      + D+LS+    +  K  YI+          + K++ +LM  IQM+LCE    +L+ +I ++     E L+               + +++I+ G+E+IHS  ++HRDLKP NIFL  ++  VKIGD GLA     M+ + K+  S+        G++ D      VGT +Y  PE L  S Y +K+DMYS+G+I FE+  +PFGT  ER H LG LR+ +I      ++ I + +++L+ S    D  +RPSA +LLQS++
Sbjct:  118 HKYLSTEGQSKRTKDG--------------LFQAQTSRYLSEFEEIATLGKGSYGKVFRVTNKLDGQEYAVKKILIKKVTRDDCMKVLREVKVLSSLQHPNIVGYHTAWMEHVQPAVT--------VDSNSLVSQTFPALD--RPSEPDGCTPEHTNSSNGSFIVFEHS---EQAEVKENRVALESISVKPSTSKVDMAQSVCTKNVRKPQNFVPCVFLGKPRSTLNKCPAANWDSSTLSEDDLSQNRLELN-KHSYIDMDTPEWSEKQENKVQFHLMLYIQMQLCE---RSLKDWIQERNSRTTEGLLISGDPCDSVDCEQALKILKKILEGVEYIHSCGIMHRDLKPRNIFLLGNECHVKIGDFGLACQNIIMDEHEKLPSSSQA------GVNPDATHTSGVGTFVYAAPEQLEGSRYDSKSDMYSIGVIAFEL-FQPFGTEMERVHTLGELRQGKIPNTLSTNWPILAKYIKLLTS---SDPSMRPSAPQLLQSDL 576

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Zebrafish
Match: eif2ak1 (eukaryotic translation initiation factor 2-alpha kinase 1 [Source:ZFIN;Acc:ZDB-GENE-061013-268])

HSP 1 Score: 177.948 bits (450), Expect = 5.971e-46
Identity = 155/504 (30.75%), Postives = 252/504 (50.00%), Query Frame = 1

Query: 1489 HPFLTCENLSKNSRQNSQTVDSAQDLTPWHLFE-KSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKK-AARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSSAISVIKPETTDSLLLDFQDKKNSIMFSANVNFSQIEHCSR------HFDLESFXXXXXXXXXXXXVELKDQSPRYKQ---PSVEISFGYSDNDDNPLDNHHKKN-TPDELSKQVKSMRLKSGYINDSQNNPFHGNKTKIEKYLM--IQMELCEGQYNTLQKYIDDQKLYMNEKLIW--------------RFIREIVSGLEHIHSKNMIHRDLKPHNIFL-DSQKRVKIGDLGLA-----MNFNNKVTESANQEYVKDNGLSNDV-ENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNI 2895
            H +L+ E  SK ++                LF+ ++SR   EFE I  LG+G++G V +V N  D Q YA+K++ +  + +    + ++EV++LSSL H NIV Y  +W E +Q + +        +DS+S+ + T  A+   +P   +    +  +       S+N +F   EH  R         LES S + S S  D +  +  ++ R  Q   P V +    S  +  P  N      + D+LS+    +  K  YI+          + K++ +LM  IQM+LCE    +L+ +I ++     E L+               + +++I+ G+E+IHS  ++HRDLKP NIFL  ++  VKIGD GLA     M+ + K+  S+        G++ D      VGT +Y  PE L  S Y +K+DMYS+G+I FE+  +PFGT  ER H LG LR+ +I      ++ I + +++L+ S    D  +RPSA +LLQS++
Sbjct:  118 HKYLSTEGQSKRTKDG--------------LFQAQTSRYLSEFEEIATLGKGSYGKVFRVTNKLDGQEYAVKKILIKKVTRDDCMKVLREVKVLSSLQHPNIVGYHTAWMEHVQPAVT--------VDSNSLVSQTFPALD--RPSEPNGCTPEHTN-------SSNGSFIVFEHSERAEVKENQVALESISVNPSTSKVDMAQSVCPKNVRKPQNFVPCVFLGKPRSTLNKCPAANWDSSTLSEDDLSQNRLELN-KHSYIDMDTPEWSEKQENKVQFHLMLYIQMQLCE---RSLKDWIQERNSRTTEGLLMSGDPCDSVDCEQALKILKKILEGVEYIHSCGIMHRDLKPRNIFLLGNECHVKIGDFGLACQNIIMDEHEKLPSSSQA------GVNPDATHTSGVGTFVYAAPEQLEGSRYDSKSDMYSIGVIAFEL-FQPFGTEMERVHTLGELRQGKIPNTLSTNWPILAKYIKLLTS---SDPSMRPSAPQLLQSDL 576

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Zebrafish
Match: eif2ak1 (eukaryotic translation initiation factor 2-alpha kinase 1 [Source:ZFIN;Acc:ZDB-GENE-061013-268])

HSP 1 Score: 177.563 bits (449), Expect = 1.621e-45
Identity = 155/504 (30.75%), Postives = 252/504 (50.00%), Query Frame = 1

Query: 1489 HPFLTCENLSKNSRQNSQTVDSAQDLTPWHLFE-KSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKK-AARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSSAISVIKPETTDSLLLDFQDKKNSIMFSANVNFSQIEHCSR------HFDLESFXXXXXXXXXXXXVELKDQSPRYKQ---PSVEISFGYSDNDDNPLDNHHKKN-TPDELSKQVKSMRLKSGYINDSQNNPFHGNKTKIEKYLM--IQMELCEGQYNTLQKYIDDQKLYMNEKLIW--------------RFIREIVSGLEHIHSKNMIHRDLKPHNIFL-DSQKRVKIGDLGLA-----MNFNNKVTESANQEYVKDNGLSNDV-ENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNI 2895
            H +L+ E  SK ++                LF+ ++SR   EFE I  LG+G++G V +V N  D Q YA+K++ +  + +    + ++EV++LSSL H NIV Y  +W E +Q + +        +DS+S+ + T  A+   +P   +    +  +       S+N +F   EH  R         LES S + S S  D +  +  ++ R  Q   P V +    S  +  P  N      + D+LS+    +  K  YI+          + K++ +LM  IQM+LCE    +L+ +I ++     E L+               + +++I+ G+E+IHS  ++HRDLKP NIFL  ++  VKIGD GLA     M+ + K+  S+        G++ D      VGT +Y  PE L  S Y +KA+MYS+G+I FE+  +PFGT  ER H LG LR+ +I      ++ I + +++L+ S    D  +RPSA +LLQS++
Sbjct:  165 HKYLSTEGQSKRTKDG--------------LFQAQTSRYLSEFEEIATLGKGSYGKVFRVTNKLDGQEYAVKKILIKKVTRDDCMKVLREVKVLSSLQHPNIVGYHTAWMEHVQPAVT--------VDSNSLVSQTFPALD--RPSEPNGCTPEHTN-------SSNGSFIVFEHSERAEVKENQVALESISVNPSTSKVDMAQSVCPKNVRKPQNFVPCVFLGKPRSTLNKCPAANWDSSTLSEDDLSQNRLELN-KHSYIDMDTPEWSEKQENKVQFHLMLYIQMQLCE---RSLKDWIQERNSRTTEGLLMSGDPCDSVDCEQALKILKKILEGVEYIHSCGIMHRDLKPRNIFLLGNECHVKIGDFGLACQNIIMDEHEKLPSSSQA------GVNPDATHTSGVGTFVYAAPEQLEGSRYDSKANMYSIGVIAFEL-FQPFGTEMERVHTLGELRQGKIPNTLSTNWPILAKYIKLLTS---SDPSMRPSAPQLLQSDL 623

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Zebrafish
Match: pkz (protein kinase containing Z-DNA binding domains [Source:ZFIN;Acc:ZDB-GENE-050301-2])

HSP 1 Score: 104.375 bits (259), Expect = 1.304e-22
Identity = 73/202 (36.14%), Postives = 104/202 (51.49%), Query Frame = 1

Query: 2290 YLMIQMELCEGQYNTLQKYIDDQKLYMNEKL---IWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDE--KHFKIKSSFVELIKSLLNHDQLLRPSASEL 2880
            YL IQME CEG   TL  +I ++     +++   I R   EI SG+E+IHS N+IHRDLKP NI  +  K VKIGD GL     N   +   +   K             GT  Y+ PE  N  +Y  K D++ LG++ FEM  K   +G ER  I  NLR  ++  PD+  + +  ++ F+E + S+   D   RP A ++
Sbjct:  249 YLFIQMEFCEG--GTLTSWIGERNYGGKQRIPLEIHRIFYEISSGVEYIHSNNLIHRDLKPDNILFNDGK-VKIGDFGLVAAQKNPSGDPIERSKRK-------------GTPTYMSPEQENLRNYDEKTDIFPLGLMWFEMLWK-ISSGMERAEIWTNLRNQKL--PDQFCERYTAENKFIEKMLSVTPED---RPHAKDI 428

HSP 2 Score: 60.4622 bits (145), Expect = 1.135e-8
Identity = 32/89 (35.96%), Postives = 52/89 (58.43%), Query Frame = 1

Query: 1594 SRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPN 1860
            S + + F++I +LG+G +G V KV++  D++ YA+KRV  N      +    EV+ L+ L+H NIVRYF  W +     S++  N + N
Sbjct:  164 SGMSQTFDVIEELGDGGYGFVCKVKHKIDDKIYAVKRVEFN------SEAEPEVKALARLDHPNIVRYFTCWPDSDNWMSNQETNEKSN 246

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Xenopus
Match: eif2ak4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:NCBI gene;Acc:100216307])

HSP 1 Score: 382.104 bits (980), Expect = 7.288e-108
Identity = 369/1296 (28.47%), Postives = 589/1296 (45.45%), Query Frame = 1

Query:  721 CDGQ---HRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQI---------RFSDLKRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKR---------FEELKGRGHSNERTGASTIKKGSKRYDLMKL---------GVLAKELFFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFLT-----------CENLSKNSRQNSQTVDSAQDLTPWHLFEKS--SRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSSAISVIKPETTDSLLLDFQDKKNSIMFSANVNFSQIEHCSRHFDLE-SFXXXXXXXXXXXXVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPFHGNK--TKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVE-------NGPVGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPK----FINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDYDSPN-KTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRAS--------LHD-MVSKDVKNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNRIHEGYDVI-NSAFKELKSVEEKLKHFTEDLCFCVNFRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEI-YDKDEHGEE 4374
            C GQ   HR K +  +  +  + +NG     G  V IY+          +F   + K+  +     +   E +F+ L KL+HP+++ Y   R +  E  I         +  +  +  G KL    E               + + ++ +Y   +L A+ YLH  ++    +   S+L+  D ++K+ ++ + KR         FE+ K R  S +   + + KKG    D+ KL         G +AKE +   +P      +D+P+D  DF+  C           ++L  H FL            C +        +    S+   + +H+  +   SR   EFE +  LG+GAFG V+KV+N  D  YYA+KRV++N   K+  R   EV LLS LNHENIVRY+ +W E+      E   + P  ++   +  T     ++K +    LL D ++   + + +++V +S    C R      +  D DS  D D   E    SP +           +D+D   +  +    +PD+  +  K         N+ ++     N+  T+   YL IQME CE   +TL+  ID Q LYM+   +WR  REI+ GL +IH + MIHRDLKP NIFLDS+  VKIGD GLA +     TESA  E  +D   S+ V+        G VGT +Y+ PE+    ++SY  K D+YSLGII+FEM ++P  T SER  +L  LR+  I+FP +          ++I  LLNHD   RP+A ELL+ +++PPP+ +E EL + L     N     Y+ M+ ++F++  S    Y     Y S     I + +S                       L   V   +  IF  HGAV+  APLL P+    F N+ES  F  +D  G++V LP NLR++FA F+  N I+  KRY+I  +++ + + R HP+E  E AFDI+T   +     AE +Y +++I ++    +    ++ +N+T+L  A++ H  +           L+D M+ K  K      F +L+   +  + +     +     L DLE    +   +   G  V+     K+L+ V   +K     L   VNF   LV     + G+VFQ        F+  IK  RR + +          +IL++GG Y   + + + P  G  G       AVG SI +  +  ++ + E+ I +   D+ V  + +  +N A+ +                 +LWAA I+AEI Y+  E  +E
Sbjct:  291 CSGQIVVHRGKCLGKDELLGKSVYNGLETASGHFVVIYEWVLQWQKKMGKFLTNQEKEKIEKCKKQIQGTETEFSSLIKLSHPNIVHYLAMRIKEKEDSIS--------VGIIAEHVNGTKLSSYLEQGVP-----------VPLDQLRHYTAQLLSALDYLHSNSVVHKVMCASSVLVTADGKVKLTDYSLSKRLADICKEDVFEQTKVR-FSEDALPSKSGKKG----DIWKLGLLLLSLSQGNIAKE-YPVKVP------SDLPADFQDFLQKCVCLEDKERWTTQQLLSHSFLNPPPEKSPVTEECSDDYPGDCTETVIPRSSVSNSTFHIESQRQFSRYFNEFEELQMLGKGAFGAVIKVQNKLDGCYYAVKRVQINPASKQFRRIKGEVTLLSRLNHENIVRYYNAWIEK-----HEHPTISPKCEAHEEKKVTVKPEQIVKNDL--GLLDDIEENAPAPILTSSVEWST--SCERSSSAHCTNGDQDSSDDDDDDDEEGVFSPSF--------LPGTDSDSEIIFENEDDISPDQDEEYDK---------NNVEDRKAGANEIVTQAVHYLYIQMEYCEK--STLRDTID-QGLYMDVGRLWRLFREILDGLTYIHEQGMIHRDLKPVNIFLDSEDHVKIGDFGLATDHPASYTESAQGE--EDQAHSDLVKVDPEGKLTGMVGTALYVSPEVQGNTKASYNQKVDLYSLGIILFEMSYRPMDTSSERISVLSRLRQALIVFPSDFENPENEKQKKVITWLLNHDPASRPTAMELLKCDLLPPPQLEESELHEVLHHTLANIDGKAYRTMMSQIFAQRISPAMDY----TYDSDILKGIFSVRSA---------------------RLHHHVCEAVCRIFKRHGAVKLHAPLLMPRGKAGFDNSESACF--MDHSGMLVTLPYNLRISFARFVARNNITSLKRYSIERVFRPRKLNRCHPKELTECAFDIITSAANCYLPIAETIYTIYEIIQEFPVLQERNYSIYVNHTSLLKAILLHCGVPEDKLTQVYNILYDAMIEKLTKREVEAKFCNLSLSENSFQLSRLYRFIEQKGDLPDLEA-MMVPLTKQKTGVGVLAKQGLKDLEGVIGLVKRLGIKLQIVVNF--GLVYKMQQHSGIVFQ--------FVAFIK--RRQRTVP---------EILAAGGRYDHLISQFRGPQTGAMG----LPCAVGVSIAIDKISAALSTAEEHISS--CDLLVVSAGQMSLNRAIGI---------------TQKLWAAGITAEIMYEWSESQDE 1454

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Xenopus
Match: eif2ak4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:NCBI gene;Acc:100216307])

HSP 1 Score: 382.104 bits (980), Expect = 9.074e-108
Identity = 369/1296 (28.47%), Postives = 589/1296 (45.45%), Query Frame = 1

Query:  721 CDGQ---HRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQI---------RFSDLKRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKR---------FEELKGRGHSNERTGASTIKKGSKRYDLMKL---------GVLAKELFFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFLT-----------CENLSKNSRQNSQTVDSAQDLTPWHLFEKS--SRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSSAISVIKPETTDSLLLDFQDKKNSIMFSANVNFSQIEHCSRHFDLE-SFXXXXXXXXXXXXVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPFHGNK--TKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVE-------NGPVGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPK----FINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDYDSPN-KTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRAS--------LHD-MVSKDVKNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNRIHEGYDVI-NSAFKELKSVEEKLKHFTEDLCFCVNFRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEI-YDKDEHGEE 4374
            C GQ   HR K +  +  +  + +NG     G  V IY+          +F   + K+  +     +   E +F+ L KL+HP+++ Y   R +  E  I         +  +  +  G KL    E               + + ++ +Y   +L A+ YLH  ++    +   S+L+  D ++K+ ++ + KR         FE+ K R  S +   + + KKG    D+ KL         G +AKE +   +P      +D+P+D  DF+  C           ++L  H FL            C +        +    S+   + +H+  +   SR   EFE +  LG+GAFG V+KV+N  D  YYA+KRV++N   K+  R   EV LLS LNHENIVRY+ +W E+      E   + P  ++   +  T     ++K +    LL D ++   + + +++V +S    C R      +  D DS  D D   E    SP +           +D+D   +  +    +PD+  +  K         N+ ++     N+  T+   YL IQME CE   +TL+  ID Q LYM+   +WR  REI+ GL +IH + MIHRDLKP NIFLDS+  VKIGD GLA +     TESA  E  +D   S+ V+        G VGT +Y+ PE+    ++SY  K D+YSLGII+FEM ++P  T SER  +L  LR+  I+FP +          ++I  LLNHD   RP+A ELL+ +++PPP+ +E EL + L     N     Y+ M+ ++F++  S    Y     Y S     I + +S                       L   V   +  IF  HGAV+  APLL P+    F N+ES  F  +D  G++V LP NLR++FA F+  N I+  KRY+I  +++ + + R HP+E  E AFDI+T   +     AE +Y +++I ++    +    ++ +N+T+L  A++ H  +           L+D M+ K  K      F +L+   +  + +     +     L DLE    +   +   G  V+     K+L+ V   +K     L   VNF   LV     + G+VFQ        F+  IK  RR + +          +IL++GG Y   + + + P  G  G       AVG SI +  +  ++ + E+ I +   D+ V  + +  +N A+ +                 +LWAA I+AEI Y+  E  +E
Sbjct:  291 CSGQIVVHRGKCLGKDELLGKSVYNGLETASGHFVVIYEWVLQWQKKMGKFLTNQEKEKIEKCKKQIQGTETEFSSLIKLSHPNIVHYLAMRIKEKEDSIS--------VGIIAEHVNGTKLSSYLEQGVP-----------VPLDQLRHYTAQLLSALDYLHSNSVVHKVMCASSVLVTADGKVKLTDYSLSKRLADICKEDVFEQTKVR-FSEDALPSKSGKKG----DIWKLGLLLLSLSQGNIAKE-YPVKVP------SDLPADFQDFLQKCVCLEDKERWTTQQLLSHSFLNPPPEKSPVTEECSDDYPGDCTETVIPRSSVSNSTFHIESQRQFSRYFNEFEELQMLGKGAFGAVIKVQNKLDGCYYAVKRVQINPASKQFRRIKGEVTLLSRLNHENIVRYYNAWIEK-----HEHPTISPKCEAHEEKKVTVKPEQIVKNDL--GLLDDIEENAPAPILTSSVEWST--SCERSSSAHCTNGDQDSSDDDDDDDEEGVFSPSF--------LPGTDSDSEIIFENEDDISPDQDEEYDK---------NNVEDRKAGANEIVTQAVHYLYIQMEYCEK--STLRDTID-QGLYMDVGRLWRLFREILDGLTYIHEQGMIHRDLKPVNIFLDSEDHVKIGDFGLATDHPASYTESAQGE--EDQAHSDLVKVDPEGKLTGMVGTALYVSPEVQGNTKASYNQKVDLYSLGIILFEMSYRPMDTSSERISVLSRLRQALIVFPSDFENPENEKQKKVITWLLNHDPASRPTAMELLKCDLLPPPQLEESELHEVLHHTLANIDGKAYRTMMSQIFAQRISPAMDY----TYDSDILKGIFSVRSA---------------------RLHHHVCEAVCRIFKRHGAVKLHAPLLMPRGKAGFDNSESACF--MDHSGMLVTLPYNLRISFARFVARNNITSLKRYSIERVFRPRKLNRCHPKELTECAFDIITSAANCYLPIAETIYTIYEIIQEFPVLQERNYSIYVNHTSLLKAILLHCGVPEDKLTQVYNILYDAMIEKLTKREVEAKFCNLSLSENSFQLSRLYRFIEQKGDLPDLEA-MMVPLTKQKTGVGVLAKQGLKDLEGVIGLVKRLGIKLQIVVNF--GLVYKMQQHSGIVFQ--------FVAFIK--RRQRTVP---------EILAAGGRYDHLISQFRGPQTGAMG----LPCAVGVSIAIDKISAALSTAEEHISS--CDLLVVSAGQMSLNRAIGI---------------TQKLWAAGITAEIMYEWSESQDE 1454

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Xenopus
Match: eif2ak4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:NCBI gene;Acc:100216307])

HSP 1 Score: 377.867 bits (969), Expect = 2.148e-106
Identity = 368/1307 (28.16%), Postives = 587/1307 (44.91%), Query Frame = 1

Query:  721 CDGQ---HRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQI---------RFSDLKRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKR---------FEELKGRGHSNERTGASTIKKGSKRYDLMKL---------GVLAKELFFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFLT-----------CENLSKNSRQNSQTVDSAQDLTPWHLFEKS--SRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSSAISVIKPETTDSLLLDFQDKKNSIMFSANVNFSQIEHCSRHFDLE-SFXXXXXXXXXXXXVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPFHGNK--TKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVE------------------NGPVGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPK----FINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDYDSPN-KTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRAS--------LHD-MVSKDVKNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNRIHEGYDVI-NSAFKELKSVEEKLKHFTEDLCFCVNFRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEI-YDKDEHGEE 4374
            C GQ   HR K +  +  +  + +NG     G  V IY+          +F   + K+  +     +   E +F+ L KL+HP+++ Y   R +  E  I         +  +  +  G KL    E               + + ++ +Y   +L A+ YLH  ++    +   S+L+  D ++K+ ++ + KR         FE+ K R  S +   + + KKG    D+ KL         G +AKE +   +P      +D+P+D  DF+  C           ++L  H FL            C +        +    S+   + +H+  +   SR   EFE +  LG+GAFG V+KV+N  D  YYA+KRV++N   K+  R   EV LLS LNHENIVRY+ +W E+      E   + P  ++   +  T     ++K +    LL D ++   + + +++V +S    C R      +  D DS  D D   E    SP +           +D+D   +  +    +PD+               N+ ++     N+  T+   YL IQME CE   +TL+  ID Q LYM+   +WR  REI+ GL +IH + MIHRDLKP NIFLDS+  VKIGD GLA +     TESA  E  +D   S+ V+                   G VGT +Y+ PE+    ++SY  K D+YSLGII+FEM ++P  T SER  +L  LR+  I+FP +          ++I  LLNHD   RP+A ELL+ +++PPP+ +E EL + L     N     Y+ M+ ++F++  S    Y     Y S     I + +S                       L   V   +  IF  HGAV+  APLL P+    F N+ES  F  +D  G++V LP NLR++FA F+  N I+  KRY+I  +++ + + R HP+E  E AFDI+T   +     AE +Y +++I ++    +    ++ +N+T+L  A++ H  +           L+D M+ K  K      F +L+   +  + +     +     L DLE    +   +   G  V+     K+L+ V   +K     L   VNF   LV     + G+VFQ        F+  IK  RR + +          +IL++GG Y   + + + P  G  G       AVG SI +  +  ++ + E+ I +   D+ V  + +  +N A+ +                 +LWAA I+AEI Y+  E  +E
Sbjct:  291 CSGQIVVHRGKCLGKDELLGKSVYNGLETASGHFVVIYEWVLQWQKKMGKFLTNQEKEKIEKCKKQIQGTETEFSSLIKLSHPNIVHYLAMRIKEKEDSIS--------VGIIAEHVNGTKLSSYLEQGVP-----------VPLDQLRHYTAQLLSALDYLHSNSVVHKVMCASSVLVTADGKVKLTDYSLSKRLADICKEDVFEQTKVR-FSEDALPSKSGKKG----DIWKLGLLLLSLSQGNIAKE-YPVKVP------SDLPADFQDFLQKCVCLEDKERWTTQQLLSHSFLNPPPEKSPVTEECSDDYPGDCTETVIPRSSVSNSTFHIESQRQFSRYFNEFEELQMLGKGAFGAVIKVQNKLDGCYYAVKRVQINPASKQFRRIKGEVTLLSRLNHENIVRYYNAWIEK-----HEHPTISPKCEAHEEKKVTVKPEQIVKNDL--GLLDDIEENAPAPILTSSVEWST--SCERSSSAHCTNGDQDSSDDDDDDDEEGVFSPSF--------LPGTDSDSEIIFENEDDISPDQ---------------NNVEDRKAGANEIVTQAVHYLYIQMEYCEK--STLRDTID-QGLYMDVGRLWRLFREILDGLTYIHEQGMIHRDLKPVNIFLDSEDHVKIGDFGLATDHPASYTESAQGE--EDQAHSDLVKVDPEDRASTPGRNTQGKLTGMVGTALYVSPEVQGNTKASYNQKVDLYSLGIILFEMSYRPMDTSSERISVLSRLRQALIVFPSDFENPENEKQKKVITWLLNHDPASRPTAMELLKCDLLPPPQLEESELHEVLHHTLANIDGKAYRTMMSQIFAQRISPAMDY----TYDSDILKGIFSVRSA---------------------RLHHHVCEAVCRIFKRHGAVKLHAPLLMPRGKAGFDNSESACF--MDHSGMLVTLPYNLRISFARFVARNNITSLKRYSIERVFRPRKLNRCHPKELTECAFDIITSAANCYLPIAETIYTIYEIIQEFPVLQERNYSIYVNHTSLLKAILLHCGVPEDKLTQVYNILYDAMIEKLTKREVEAKFCNLSLSENSFQLSRLYRFIEQKGDLPDLEA-MMVPLTKQKTGVGVLAKQGLKDLEGVIGLVKRLGIKLQIVVNF--GLVYKMQQHSGIVFQ--------FVAFIK--RRQRTVP---------EILAAGGRYDHLISQFRGPQTGAMG----LPCAVGVSIAIDKISAALSTAEEHISS--CDLLVVSAGQMSLNRAIGI---------------TQKLWAAGITAEIMYEWSESQDE 1459

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Xenopus
Match: eif2ak4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:NCBI gene;Acc:100216307])

HSP 1 Score: 281.567 bits (719), Expect = 4.160e-76
Identity = 226/716 (31.56%), Postives = 345/716 (48.18%), Query Frame = 1

Query: 2302 QMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVE-------NGPVGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPK----FINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDYDSPN-KTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRAS--------LHD-MVSKDVKNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNRIHEGYDVI-NSAFKELKSVEEKLKHFTEDLCFCVNFRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEI-YDKDEHGEE 4374
            QME CE   +TL+  ID Q LYM+   +WR  REI+ GL +IH + MIHRDLKP NIFLDS+  VKIGD GLA +            YVKD   S+ V+        G VGT +Y+ PE+    ++SY  K D+YSLGII+FEM ++P  T SER  +L  LR+  I+FP +          ++I  LLNHD   RP+A ELL+ +++PPP+ +E EL + L     N     Y+ M+ ++F++  S    Y     Y S     I + +S                       L   V   +  IF  HGAV+  APLL P+    F N+ES  F  +D  G++V LP NLR++FA F+  N I+  KRY+I  +++ + + R HP+E  E AFDI+T   +     AE +Y +++I ++    +    ++ +N+T+L  A++ H  +           L+D M+ K  K      F +L+   +  + +     +     L DLE    +   +   G  V+     K+L+ V   +K     L   VNF   LV     + G+VFQ        F+  IK  RR + +          +IL++GG Y   + + + P  G  G       AVG SI +  +  ++ + E+ I +   D+ V  + +  +N A+ +                 +LWAA I+AEI Y+  E  +E
Sbjct:  609 QMEYCEK--STLRDTID-QGLYMDVGRLWRLFREILDGLTYIHEQGMIHRDLKPVNIFLDSEDHVKIGDFGLATDHP--------ASYVKDQAHSDLVKVDPEGKLTGMVGTALYVSPEVQGNTKASYNQKVDLYSLGIILFEMSYRPMDTSSERISVLSRLRQALIVFPSDFENPENEKQKKVITWLLNHDPASRPTAMELLKCDLLPPPQLEESELHEVLHHTLANIDGKAYRTMMSQIFAQRISPAMDY----TYDSDILKGIFSVRSA---------------------RLHHHVCEAVCRIFKRHGAVKLHAPLLMPRGKAGFDNSESACF--MDHSGMLVTLPYNLRISFARFVARNNITSLKRYSIERVFRPRKLNRCHPKELTECAFDIITSAANCYLPIAETIYTIYEIIQEFPVLQERNYSIYVNHTSLLKAILLHCGVPEDKLTQVYNILYDAMIEKLTKREVEAKFCNLSLSENSFQLSRLYRFIEQKGDLPDLEA-MMVPLTKQKTGVGVLAKQGLKDLEGVIGLVKRLGIKLQIVVNF--GLVYKMQQHSGIVFQ--------FVAFIK--RRQRTVP---------EILAAGGRYDHLISQFRGPQTGAMG----LPCAVGVSIAIDKISAALSTAEEHISS--CDLLVVSAGQMSLNRAIGI---------------TQKLWAAGITAEIMYEWSESQDE 1243

HSP 2 Score: 111.694 bits (278), Expect = 7.986e-24
Identity = 106/405 (26.17%), Postives = 181/405 (44.69%), Query Frame = 1

Query:  895 EEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKR---------FEELKGRGHSNERTGASTIKKGSKRYDLMKL---------GVLAKELFFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFLT-----------CENLSKNSRQNSQTVDSAQDLTPWHLFEKS--SRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSSAISVIKPETTDSLLLDFQDKKNSIMFSANVNFS 1995
            E +F+ L KL+HP+++ Y   R +  E  I         +  +  +  G KL    E               + + ++ +Y   +L A+ YLH  ++    +   S+L+  D ++K+ ++ + KR         FE+ K R  S +   + + KKG    D+ KL         G +AKE +   +P      +D+P+D  DF+  C           ++L  H FL            C +        +    S+   + +H+  +   SR   EFE +  LG+GAFG V+KV+N  D  YYA+KRV++N   K+  R   EV LLS LNHENIVRY+ +W E+      E   + P  ++   +  T     ++K +    LL D ++   + + +++V +S
Sbjct:  186 ETEFSSLIKLSHPNIVHYLAMRIKEKEDSIS--------VGIIAEHVNGTKLSSYLEQGVP-----------VPLDQLRHYTAQLLSALDYLHSNSVVHKVMCASSVLVTADGKVKLTDYSLSKRLADICKEDVFEQTKVR-FSEDALPSKSGKKG----DIWKLGLLLLSLSQGNIAKE-YPVKVP------SDLPADFQDFLQKCVCLEDKERWTTQQLLSHSFLNPPPEKSPVTEECSDDYPGDCTETVIPRSSVSNSTFHIESQRQFSRYFNEFEELQMLGKGAFGAVIKVQNKLDGCYYAVKRVQINPASKQFRRIKGEVTLLSRLNHENIVRYYNAWIEK-----HEHPTISPKCEAHEEKKVTVKPEQIVKNDL--GLLDDIEENAPAPILTSSVEWS 552

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Xenopus
Match: eif2ak4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:NCBI gene;Acc:100216307])

HSP 1 Score: 281.182 bits (718), Expect = 6.484e-76
Identity = 226/716 (31.56%), Postives = 345/716 (48.18%), Query Frame = 1

Query: 2302 QMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVE-------NGPVGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPK----FINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDYDSPN-KTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRAS--------LHD-MVSKDVKNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNRIHEGYDVI-NSAFKELKSVEEKLKHFTEDLCFCVNFRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEI-YDKDEHGEE 4374
            QME CE   +TL+  ID Q LYM+   +WR  REI+ GL +IH + MIHRDLKP NIFLDS+  VKIGD GLA +            YVKD   S+ V+        G VGT +Y+ PE+    ++SY  K D+YSLGII+FEM ++P  T SER  +L  LR+  I+FP +          ++I  LLNHD   RP+A ELL+ +++PPP+ +E EL + L     N     Y+ M+ ++F++  S    Y     Y S     I + +S                       L   V   +  IF  HGAV+  APLL P+    F N+ES  F  +D  G++V LP NLR++FA F+  N I+  KRY+I  +++ + + R HP+E  E AFDI+T   +     AE +Y +++I ++    +    ++ +N+T+L  A++ H  +           L+D M+ K  K      F +L+   +  + +     +     L DLE    +   +   G  V+     K+L+ V   +K     L   VNF   LV     + G+VFQ        F+  IK  RR + +          +IL++GG Y   + + + P  G  G       AVG SI +  +  ++ + E+ I +   D+ V  + +  +N A+ +                 +LWAA I+AEI Y+  E  +E
Sbjct:  699 QMEYCEK--STLRDTID-QGLYMDVGRLWRLFREILDGLTYIHEQGMIHRDLKPVNIFLDSEDHVKIGDFGLATDHPAS--------YVKDQAHSDLVKVDPEGKLTGMVGTALYVSPEVQGNTKASYNQKVDLYSLGIILFEMSYRPMDTSSERISVLSRLRQALIVFPSDFENPENEKQKKVITWLLNHDPASRPTAMELLKCDLLPPPQLEESELHEVLHHTLANIDGKAYRTMMSQIFAQRISPAMDY----TYDSDILKGIFSVRSA---------------------RLHHHVCEAVCRIFKRHGAVKLHAPLLMPRGKAGFDNSESACF--MDHSGMLVTLPYNLRISFARFVARNNITSLKRYSIERVFRPRKLNRCHPKELTECAFDIITSAANCYLPIAETIYTIYEIIQEFPVLQERNYSIYVNHTSLLKAILLHCGVPEDKLTQVYNILYDAMIEKLTKREVEAKFCNLSLSENSFQLSRLYRFIEQKGDLPDLEA-MMVPLTKQKTGVGVLAKQGLKDLEGVIGLVKRLGIKLQIVVNF--GLVYKMQQHSGIVFQ--------FVAFIK--RRQRTVP---------EILAAGGRYDHLISQFRGPQTGAMG----LPCAVGVSIAIDKISAALSTAEEHISS--CDLLVVSAGQMSLNRAIGI---------------TQKLWAAGITAEIMYEWSESQDE 1333

HSP 2 Score: 134.42 bits (337), Expect = 1.024e-30
Identity = 158/679 (23.27%), Postives = 284/679 (41.83%), Query Frame = 1

Query:  124 QSDEVKALRSIFGKEFKLIRPKNLKKKYEPPLVSVLINVDSSNLNSALSLRISFDCPQNYPFDIPKISIYDAVNVPTNLVEQLLIILNEKSNLLKGYSMIYNLVEEARCIIIKHFETDHKSVNSILTINK-------VLNEDEIMQ-TETKQSEFLKNLLRQRRDSARXXXXXXXXXXXCDHSYKTDELEFEHCSDRSSGCLVYKGICDGQHRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQI---------RFSDLKRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKR---------FEELKGRGHSNERTGASTIKKGSKRYDLMKL---------GVLAKELFFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFLT-----------CENLSKNSRQNSQTVDSAQDLTPWHLFEKS--SRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSSAISVIKPETTDSLLLDFQDKKNSIMFSANVNFS 1995
            Q +E++ L SI+  +F  +R  +  K  +PP V + +    + ++  + L +   CPQNYP   P+I + +   +    V  L   L + +  L G  MIY L +  +C + ++     KS +  +  N         L E+  MQ  + K+ +  + +L + +     +K+ +++ E      + DEL                                 +  + +NG     G  V IY+          +F   + K+  +     +   E +F+ L KL+HP+++ Y   R +  E  I         +  +  +  G KL    E               + + ++ +Y   +L A+ YLH  ++    +   S+L+  D ++K+ ++ + KR         FE+ K R  S +   + + KKG    D+ KL         G +AKE +   +P      +D+P+D  DF+  C           ++L  H FL            C +        +    S+   + +H+  +   SR   EFE +  LG+GAFG V+KV+N  D  YYA+KRV++N   K+  R   EV LLS LNHENIVRY+ +W E+      E   + P  ++   +  T     ++K +    LL D ++   + + +++V +S
Sbjct:   37 QENELEVLESIYSGDFADLRKNSAWKVQQPPEVIIALRPQGNEVHVKVDLWVK--CPQNYPDVPPEIKLKNIQGLSNENVNSLTSSLEDLAKQLCGEVMIYQLADHVQCFLTEYNHPPPKSFHEEMLKNLQKQQEKLALEEERRMQEIKMKEEQTRREILNEIQKREEEKKEEKRRKEVAKQVSRKDEL---------------------------------LGKSVYNGLETASGHFVVIYEWVLQWQKKMGKFLTNQEKEKIEKCKKQIQGTETEFSSLIKLSHPNIVHYLAMRIKEKEDSIS--------VGIIAEHVNGTKLSSYLEQGVP-----------VPLDQLRHYTAQLLSALDYLHSNSVVHKVMCASSVLVTADGKVKLTDYSLSKRLADICKEDVFEQTKVR-FSEDALPSKSGKKG----DIWKLGLLLLSLSQGNIAKE-YPVKVP------SDLPADFQDFLQKCVCLEDKERWTTQQLLSHSFLNPPPEKSPVTEECSDDYPGDCTETVIPRSSVSNSTFHIESQRQFSRYFNEFEELQMLGKGAFGAVIKVQNKLDGCYYAVKRVQINPASKQFRRIKGEVTLLSRLNHENIVRYYNAWIEK-----HEHPTISPKCEAHEEKKVTVKPEQIVKNDL--GLLDDIEENAPAPILTSSVEWS 642

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Mouse
Match: Eif2ak4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:MGI Symbol;Acc:MGI:1353427])

HSP 1 Score: 275.404 bits (703), Expect = 1.760e-74
Identity = 226/717 (31.52%), Postives = 346/717 (48.26%), Query Frame = 1

Query: 2290 YLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVEN---GPVGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEII-FPDE----KHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLK--RFVTRYIESIFVSHGAVEFLAPLLSPK----FINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTD-YDSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRASLHDMVSKDVKNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNR--IHEGYDVINSAFKELKSVEEKLKHFTEDLCFCVNFRPSL-VPLE-TIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEI-YDKDEHGEE 4374
            YL IQME CE   +TL+  ID Q L+ +   +WR  REI+ GL +IH K MIHRDLKP NIFLDS   VKIGD GLA +      E    +   D  + +D      G VGT +Y+ PE+    +S+Y  K D++SLGII FEM + P  T SER  +L  LR P    FPD+    +H K KS    +I  LLNHD   RP+A ELL+S ++PPP+ +E EL + L     N     Y+ M+ ++F +  S    Y                              S I +G+F   T K  + V   I  +F  HGAV+   PLL P+    + + E+  F  +D  G++V LP +LR+ FA ++  N I   KRY I  +++ + + R HP+E +E AFDIVT   +S   TAE +Y +++I ++    +    ++ +N+T L  A++ H  I     D +S+    L    + ++T    +++   K   L   S    L + +K  + +  + +    INS  K+   V + +K+  +DL   V     L V L+ +I  GLV+++       F  +    RR + +          +IL++GG Y   + K + P       V    +AVG SI +  +  ++ ++E+ +     D+ V    +  M+ A+N               L  +LW A I+AEI YD  +  EE
Sbjct:  517 YLYIQMEYCEK--STLRDTID-QGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPDDFDDGEHTKQKS----VISWLLNHDPAKRPTAMELLKSELLPPPQMEESELHEVLHHTLANIDGKAYRTMMSQIFCQHISPAIDYTYD---------------------------SDILKGNFLIRTAKIQQLVCETIVRVFKRHGAVQLCTPLLLPRNRQIYEHNEAALF--MDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAETIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIP---EDKLSQVYVIL----YDAVTEKLTRREVEAKFCNLSLSSN--SLCRLYKFIEQKGDLQDLTPTINSLIKQKTGVAQLVKYSLKDLEDVVGLLKKLGVKLQVSINLGLVYKVQQHTGIIFQFLAFSKRRQRVVP---------EILAAGGRYDLLIPKFRGP-----QTVGPVPTAVGVSIAIDKIFAAVLNMEEPVTVSSCDLLVVSVGQMSMSRAIN---------------LTQKLWTAGITAEIMYDWSQSQEE 1159

HSP 2 Score: 99.3673 bits (246), Expect = 3.973e-20
Identity = 107/420 (25.48%), Postives = 184/420 (43.81%), Query Frame = 1

Query:  703 LVYKGICDGQHRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQ--IRFSDL------KRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKII------NYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKR---------FEELKGRGHSNERTGASTIKKGSKRYDLMKLGVLAKELFFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFLTCEN---LSKNSRQNS------QTVDSAQDLTPWHLFEKS----SRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSS 1833
            +V+KG C G         + Q+    +N      G+ V +++  +++  +      + K+  D     +   E +F+ L KL+HP+++ Y+    +  E  I        VI  +  +  G+                 +L T LS S  +       Y   +L  + YLH  ++    ++  S+L+D +  +KI ++ I KR         FE+ + R  S+      T KKG      + L  L++       P T    +D+P+D  DF+  C         + ++L  H F+  +    L + S ++S      +TV  +  L     F ++    SR   EFE +  LG+GAFG V+KV+N  D   YA+KR+ +N   +   R   EV LLS L+HENIVRY+ +W E  ++ +
Sbjct:    1 MVHKGRCVGS--------DEQLGKVVYNALETATGSFVLLHEWVLQWQKMGPCLTSQEKEKIDKCKRQIQGAETEFSSLVKLSHPNIVRYFAMNSREEEDSI--------VIDILAEHVSGI-----------------SLATHLSHSGPVPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITDYSISKRLADICKEDVFEQARVR-FSDSALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTI--PSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKLPLVEQSPEDSGGQDYIETVIPSNQLPSAAFFSETQKQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPA 384

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Mouse
Match: Eif2ak4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:MGI Symbol;Acc:MGI:1353427])

HSP 1 Score: 275.018 bits (702), Expect = 4.012e-74
Identity = 226/717 (31.52%), Postives = 346/717 (48.26%), Query Frame = 1

Query: 2290 YLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVEN---GPVGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEII-FPDE----KHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLK--RFVTRYIESIFVSHGAVEFLAPLLSPK----FINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTD-YDSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRASLHDMVSKDVKNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNR--IHEGYDVINSAFKELKSVEEKLKHFTEDLCFCVNFRPSL-VPLE-TIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEI-YDKDEHGEE 4374
            YL IQME CE   +TL+  ID Q L+ +   +WR  REI+ GL +IH K MIHRDLKP NIFLDS   VKIGD GLA +      E    +   D  + +D      G VGT +Y+ PE+    +S+Y  K D++SLGII FEM + P  T SER  +L  LR P    FPD+    +H K KS    +I  LLNHD   RP+A ELL+S ++PPP+ +E EL + L     N     Y+ M+ ++F +  S    Y                              S I +G+F   T K  + V   I  +F  HGAV+   PLL P+    + + E+  F  +D  G++V LP +LR+ FA ++  N I   KRY I  +++ + + R HP+E +E AFDIVT   +S   TAE +Y +++I ++    +    ++ +N+T L  A++ H  I     D +S+    L    + ++T    +++   K   L   S    L + +K  + +  + +    INS  K+   V + +K+  +DL   V     L V L+ +I  GLV+++       F  +    RR + +          +IL++GG Y   + K + P       V    +AVG SI +  +  ++ ++E+ +     D+ V    +  M+ A+N               L  +LW A I+AEI YD  +  EE
Sbjct:  674 YLYIQMEYCEK--STLRDTID-QGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPDDFDDGEHTKQKS----VISWLLNHDPAKRPTAMELLKSELLPPPQMEESELHEVLHHTLANIDGKAYRTMMSQIFCQHISPAIDYTYD---------------------------SDILKGNFLIRTAKIQQLVCETIVRVFKRHGAVQLCTPLLLPRNRQIYEHNEAALF--MDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAETIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIP---EDKLSQVYVIL----YDAVTEKLTRREVEAKFCNLSLSS--NSLCRLYKFIEQKGDLQDLTPTINSLIKQKTGVAQLVKYSLKDLEDVVGLLKKLGVKLQVSINLGLVYKVQQHTGIIFQFLAFSKRRQRVVP---------EILAAGGRYDLLIPKFRGP-----QTVGPVPTAVGVSIAIDKIFAAVLNMEEPVTVSSCDLLVVSVGQMSMSRAIN---------------LTQKLWTAGITAEIMYDWSQSQEE 1316

HSP 2 Score: 99.7525 bits (247), Expect = 3.150e-20
Identity = 107/420 (25.48%), Postives = 185/420 (44.05%), Query Frame = 1

Query:  703 LVYKGICDGQHRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQ--IRFSDL------KRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSIS------KIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKR---------FEELKGRGHSNERTGASTIKKGSKRYDLMKLGVLAKELFFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFLTCEN---LSKNSRQNS------QTVDSAQDLTPWHLFEKS----SRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSS 1833
            +V+KG C G         + Q+    +N      G+ V +++  +++  +      + K+  D     +   E +F+ L KL+HP+++ Y+    +  E  I        VI  +  +  G+                 +L T LS S      ++  Y   +L  + YLH  ++    ++  S+L+D +  +KI ++ I KR         FE+ + R  S+      T KKG      + L  L++       P T    +D+P+D  DF+  C         + ++L  H F+  +    L + S ++S      +TV  +  L     F ++    SR   EFE +  LG+GAFG V+KV+N  D   YA+KR+ +N   +   R   EV LLS L+HENIVRY+ +W E  ++ +
Sbjct:  158 MVHKGRCVGS--------DEQLGKVVYNALETATGSFVLLHEWVLQWQKMGPCLTSQEKEKIDKCKRQIQGAETEFSSLVKLSHPNIVRYFAMNSREEEDSI--------VIDILAEHVSGI-----------------SLATHLSHSGPVPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITDYSISKRLADICKEDVFEQARVR-FSDSALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTI--PSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKLPLVEQSPEDSGGQDYIETVIPSNQLPSAAFFSETQKQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPA 541

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Mouse
Match: Eif2ak4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:MGI Symbol;Acc:MGI:1353427])

HSP 1 Score: 275.018 bits (702), Expect = 4.759e-74
Identity = 226/717 (31.52%), Postives = 346/717 (48.26%), Query Frame = 1

Query: 2290 YLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVEN---GPVGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEII-FPDE----KHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLK--RFVTRYIESIFVSHGAVEFLAPLLSPK----FINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTD-YDSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRASLHDMVSKDVKNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNR--IHEGYDVINSAFKELKSVEEKLKHFTEDLCFCVNFRPSL-VPLE-TIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEI-YDKDEHGEE 4374
            YL IQME CE   +TL+  ID Q L+ +   +WR  REI+ GL +IH K MIHRDLKP NIFLDS   VKIGD GLA +      E    +   D  + +D      G VGT +Y+ PE+    +S+Y  K D++SLGII FEM + P  T SER  +L  LR P    FPD+    +H K KS    +I  LLNHD   RP+A ELL+S ++PPP+ +E EL + L     N     Y+ M+ ++F +  S    Y                              S I +G+F   T K  + V   I  +F  HGAV+   PLL P+    + + E+  F  +D  G++V LP +LR+ FA ++  N I   KRY I  +++ + + R HP+E +E AFDIVT   +S   TAE +Y +++I ++    +    ++ +N+T L  A++ H  I     D +S+    L    + ++T    +++   K   L   S    L + +K  + +  + +    INS  K+   V + +K+  +DL   V     L V L+ +I  GLV+++       F  +    RR + +          +IL++GG Y   + K + P       V    +AVG SI +  +  ++ ++E+ +     D+ V    +  M+ A+N               L  +LW A I+AEI YD  +  EE
Sbjct:  683 YLYIQMEYCEK--STLRDTID-QGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPDDFDDGEHTKQKS----VISWLLNHDPAKRPTAMELLKSELLPPPQMEESELHEVLHHTLANIDGKAYRTMMSQIFCQHISPAIDYTYD---------------------------SDILKGNFLIRTAKIQQLVCETIVRVFKRHGAVQLCTPLLLPRNRQIYEHNEAALF--MDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAETIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIP---EDKLSQVYVIL----YDAVTEKLTRREVEAKFCNLSLSS--NSLCRLYKFIEQKGDLQDLTPTINSLIKQKTGVAQLVKYSLKDLEDVVGLLKKLGVKLQVSINLGLVYKVQQHTGIIFQFLAFSKRRQRVVP---------EILAAGGRYDLLIPKFRGP-----QTVGPVPTAVGVSIAIDKIFAAVLNMEEPVTVSSCDLLVVSVGQMSMSRAIN---------------LTQKLWTAGITAEIMYDWSQSQEE 1325

HSP 2 Score: 99.3673 bits (246), Expect = 3.464e-20
Identity = 107/420 (25.48%), Postives = 184/420 (43.81%), Query Frame = 1

Query:  703 LVYKGICDGQHRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQ--IRFSDL------KRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKII------NYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKR---------FEELKGRGHSNERTGASTIKKGSKRYDLMKLGVLAKELFFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFLTCEN---LSKNSRQNS------QTVDSAQDLTPWHLFEKS----SRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSS 1833
            +V+KG C G         + Q+    +N      G+ V +++  +++  +      + K+  D     +   E +F+ L KL+HP+++ Y+    +  E  I        VI  +  +  G+                 +L T LS S  +       Y   +L  + YLH  ++    ++  S+L+D +  +KI ++ I KR         FE+ + R  S+      T KKG      + L  L++       P T    +D+P+D  DF+  C         + ++L  H F+  +    L + S ++S      +TV  +  L     F ++    SR   EFE +  LG+GAFG V+KV+N  D   YA+KR+ +N   +   R   EV LLS L+HENIVRY+ +W E  ++ +
Sbjct:  167 MVHKGRCVGS--------DEQLGKVVYNALETATGSFVLLHEWVLQWQKMGPCLTSQEKEKIDKCKRQIQGAETEFSSLVKLSHPNIVRYFAMNSREEEDSI--------VIDILAEHVSGI-----------------SLATHLSHSGPVPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITDYSISKRLADICKEDVFEQARVR-FSDSALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTI--PSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKLPLVEQSPEDSGGQDYIETVIPSNQLPSAAFFSETQKQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPA 550

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Mouse
Match: Eif2ak4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:MGI Symbol;Acc:MGI:1353427])

HSP 1 Score: 274.633 bits (701), Expect = 5.545e-74
Identity = 226/717 (31.52%), Postives = 346/717 (48.26%), Query Frame = 1

Query: 2290 YLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVEN---GPVGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEII-FPDE----KHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLK--RFVTRYIESIFVSHGAVEFLAPLLSPK----FINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTD-YDSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRASLHDMVSKDVKNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNR--IHEGYDVINSAFKELKSVEEKLKHFTEDLCFCVNFRPSL-VPLE-TIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEI-YDKDEHGEE 4374
            YL IQME CE   +TL+  ID Q L+ +   +WR  REI+ GL +IH K MIHRDLKP NIFLDS   VKIGD GLA +      E    +   D  + +D      G VGT +Y+ PE+    +S+Y  K D++SLGII FEM + P  T SER  +L  LR P    FPD+    +H K KS    +I  LLNHD   RP+A ELL+S ++PPP+ +E EL + L     N     Y+ M+ ++F +  S    Y                              S I +G+F   T K  + V   I  +F  HGAV+   PLL P+    + + E+  F  +D  G++V LP +LR+ FA ++  N I   KRY I  +++ + + R HP+E +E AFDIVT   +S   TAE +Y +++I ++    +    ++ +N+T L  A++ H  I     D +S+    L    + ++T    +++   K   L   S    L + +K  + +  + +    INS  K+   V + +K+  +DL   V     L V L+ +I  GLV+++       F  +    RR + +          +IL++GG Y   + K + P       V    +AVG SI +  +  ++ ++E+ +     D+ V    +  M+ A+N               L  +LW A I+AEI YD  +  EE
Sbjct:  717 YLYIQMEYCEK--STLRDTID-QGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPDDFDDGEHTKQKS----VISWLLNHDPAKRPTAMELLKSELLPPPQMEESELHEVLHHTLANIDGKAYRTMMSQIFCQHISPAIDYTYD---------------------------SDILKGNFLIRTAKIQQLVCETIVRVFKRHGAVQLCTPLLLPRNRQIYEHNEAALF--MDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAETIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIP---EDKLSQVYVIL----YDAVTEKLTRREVEAKFCNLSLSSN--SLCRLYKFIEQKGDLQDLTPTINSLIKQKTGVAQLVKYSLKDLEDVVGLLKKLGVKLQVSINLGLVYKVQQHTGIIFQFLAFSKRRQRVVP---------EILAAGGRYDLLIPKFRGP-----QTVGPVPTAVGVSIAIDKIFAAVLNMEEPVTVSSCDLLVVSVGQMSMSRAIN---------------LTQKLWTAGITAEIMYDWSQSQEE 1359

HSP 2 Score: 99.3673 bits (246), Expect = 3.999e-20
Identity = 107/420 (25.48%), Postives = 185/420 (44.05%), Query Frame = 1

Query:  703 LVYKGICDGQHRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQ--IRFSDL------KRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSIS------KIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKR---------FEELKGRGHSNERTGASTIKKGSKRYDLMKLGVLAKELFFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFLTCEN---LSKNSRQNS------QTVDSAQDLTPWHLFEKS----SRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSS 1833
            +V+KG C G         + Q+    +N      G+ V +++  +++  +      + K+  D     +   E +F+ L KL+HP+++ Y+    +  E  I        VI  +  +  G+                 +L T LS S      ++  Y   +L  + YLH  ++    ++  S+L+D +  +KI ++ I KR         FE+ + R  S+      T KKG      + L  L++       P T    +D+P+D  DF+  C         + ++L  H F+  +    L + S ++S      +TV  +  L     F ++    SR   EFE +  LG+GAFG V+KV+N  D   YA+KR+ +N   +   R   EV LLS L+HENIVRY+ +W E  ++ +
Sbjct:  201 MVHKGRCVGS--------DEQLGKVVYNALETATGSFVLLHEWVLQWQKMGPCLTSQEKEKIDKCKRQIQGAETEFSSLVKLSHPNIVRYFAMNSREEEDSI--------VIDILAEHVSGI-----------------SLATHLSHSGPVPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITDYSISKRLADICKEDVFEQARVR-FSDSALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTI--PSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKLPLVEQSPEDSGGQDYIETVIPSNQLPSAAFFSETQKQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPA 584

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Mouse
Match: Eif2ak4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:MGI Symbol;Acc:MGI:1353427])

HSP 1 Score: 274.633 bits (701), Expect = 7.408e-74
Identity = 226/717 (31.52%), Postives = 346/717 (48.26%), Query Frame = 1

Query: 2290 YLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVEN---GPVGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRP-EIIFPDE----KHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLK--RFVTRYIESIFVSHGAVEFLAPLLSPK----FINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTD-YDSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRASLHDMVSKDVKNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNR--IHEGYDVINSAFKELKSVEEKLKHFTEDLCFCVNFRPSL-VPLE-TIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEI-YDKDEHGEE 4374
            YL IQME CE   +TL+  ID Q L+ +   +WR  REI+ GL +IH K MIHRDLKP NIFLDS   VKIGD GLA +      E    +   D  + +D      G VGT +Y+ PE+    +S+Y  K D++SLGII FEM + P  T SER  +L  LR P    FPD+    +H K KS    +I  LLNHD   RP+A ELL+S ++PPP+ +E EL + L     N     Y+ M+ ++F +  S    Y                              S I +G+F   T K  + V   I  +F  HGAV+   PLL P+    + + E+  F  +D  G++V LP +LR+ FA ++  N I   KRY I  +++ + + R HP+E +E AFDIVT   +S   TAE +Y +++I ++    +    ++ +N+T L  A++ H  I     D +S+    L    + ++T    +++   K   L   S    L + +K  + +  + +    INS  K+   V + +K+  +DL   V     L V L+ +I  GLV+++       F  +    RR + +          +IL++GG Y   + K + P       V    +AVG SI +  +  ++ ++E+ +     D+ V    +  M+ A+N               L  +LW A I+AEI YD  +  EE
Sbjct:  795 YLYIQMEYCEK--STLRDTID-QGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPDDFDDGEHTKQKS----VISWLLNHDPAKRPTAMELLKSELLPPPQMEESELHEVLHHTLANIDGKAYRTMMSQIFCQHISPAIDYTYD---------------------------SDILKGNFLIRTAKIQQLVCETIVRVFKRHGAVQLCTPLLLPRNRQIYEHNEAALF--MDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAETIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIP---EDKLSQVYVIL----YDAVTEKLTRREVEAKFCNLSLSSN--SLCRLYKFIEQKGDLQDLTPTINSLIKQKTGVAQLVKYSLKDLEDVVGLLKKLGVKLQVSINLGLVYKVQQHTGIIFQFLAFSKRRQRVVP---------EILAAGGRYDLLIPKFRGP-----QTVGPVPTAVGVSIAIDKIFAAVLNMEEPVTVSSCDLLVVSVGQMSMSRAIN---------------LTQKLWTAGITAEIMYDWSQSQEE 1437

HSP 2 Score: 99.7525 bits (247), Expect = 2.832e-20
Identity = 107/420 (25.48%), Postives = 184/420 (43.81%), Query Frame = 1

Query:  703 LVYKGICDGQHRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQ--IRFSDL------KRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKII------NYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKR---------FEELKGRGHSNERTGASTIKKGSKRYDLMKLGVLAKELFFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFLTCEN---LSKNSRQNS------QTVDSAQDLTPWHLFEKS----SRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSS 1833
            +V+KG C G         + Q+    +N      G+ V +++  +++  +      + K+  D     +   E +F+ L KL+HP+++ Y+    +  E  I        VI  +  +  G+                 +L T LS S  +       Y   +L  + YLH  ++    ++  S+L+D +  +KI ++ I KR         FE+ + R  S+      T KKG      + L  L++       P T    +D+P+D  DF+  C         + ++L  H F+  +    L + S ++S      +TV  +  L     F ++    SR   EFE +  LG+GAFG V+KV+N  D   YA+KR+ +N   +   R   EV LLS L+HENIVRY+ +W E  ++ +
Sbjct:  279 MVHKGRCVGS--------DEQLGKVVYNALETATGSFVLLHEWVLQWQKMGPCLTSQEKEKIDKCKRQIQGAETEFSSLVKLSHPNIVRYFAMNSREEEDSI--------VIDILAEHVSGI-----------------SLATHLSHSGPVPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITDYSISKRLADICKEDVFEQARVR-FSDSALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTI--PSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKLPLVEQSPEDSGGQDYIETVIPSNQLPSAAFFSETQKQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPA 662

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. UniProt/SwissProt
Match: sp|Q9P2K8|E2AK4_HUMAN (eIF-2-alpha kinase GCN2 OS=Homo sapiens OX=9606 GN=EIF2AK4 PE=1 SV=3)

HSP 1 Score: 328.561 bits (841), Expect = 7.315e-90
Identity = 350/1289 (27.15%), Postives = 573/1289 (44.45%), Query Frame = 1

Query:  733 HRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQI---------RFSDLKRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKR---------FEELKGRGHSNERTGASTIKKGSKRYDLMKLGVLAKEL--------FFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFLTCEN---LSKNSRQNSQTVDSAQDLTPWHLFEKS----------SRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSSAISVIKPETTDSLLLDFQDKKNSIMFSANVNFSQIEHCSRHFDLESFXXXXXXXXXXXXVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPFHGNK----TKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVEN--GPVGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEII-----FPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLK--RFVTRYIESIFVSHGAVEFLAPLLSPK----FINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTD-YDSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRAS--------LHDMVSKDVKNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNRIHEGYDVINSAFKELKSVEEKLKHFTEDLCFCVNFRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEI-YDKDEHGEE 4374
            H+ K I  + Q+    +N      G  V +Y+           F   + K+  D     +   E +F  L KL+HP+++ Y     +  +  I      D+++++++    G+ L           +   +    + + ++  Y   +L  + YLH  ++    ++  ++L+D +  +KI ++ I KR         FE+ + R   N    A   K G K+ D+ +LG+L   L        +   IP      +D+P+D  DF+  C         + ++L  H F+  +    L + S ++S+  D  + + P +    +          SR   EFE +  LG+GAFG V+KV+N  D   YA+KR+ +N   ++  R   EV LLS L+HENIVRY+ +W E  ++ +       P  DS  +  +  +A    +P +    L   +      + S++V +S     S       F  +   S  D   +  +    + Q  +  S   SD   +  D + K    DE               + ++ N  H ++    T+   YL IQME CE   +TL+  ID Q LY +   +WR  REI+ GL +IH K MIHRDLKP NIFLDS   VKIGD GLA +      +S   +   D   S+   +  G VGT +Y+ PE+    +S+Y  K D++SLGII FEM + P  T SER  +L  LR P        F D +H K KS    +I  LLNHD   RP+A+ELL+S ++PPP+ +E EL + L     N     Y+ M+ ++FS+  S    Y                              S I +G+F   T K  + V   I  IF  HGAV+   PLL P+    + + E+  F  +D  G++V LP +LR+ FA ++  N I   KRY I  +++ + + R HP+E +E AFDIVT   +S   TAEI+Y +++I ++    +    ++ +N+T L  A++ H  I           L+D V++ +   + +      +L+      +   +   G  L+DL         +      ++    K+L+ V   LK     L   +N    LV     ++G++FQ        F+  IK  RR + +          +IL++GG Y   + + + P     G V    +A+G SI +  +  ++ ++E+ +     D+ V    +  M+ A+N               L  +LW A I+AEI YD  +  EE
Sbjct:  281 HKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVRYLAMNLKEQDDSIV----VDILVEHIS----GVSL-----------AAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRLADICKEDVFEQTRVRFSDN----ALPYKTG-KKGDVWRLGLLLLSLSQGQECGEYPVTIP------SDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGT---PPPDSGPLAKDDRAARG--QPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSAS---ARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQDE---------------DCNEKNGCHESEPSVTTEAVHYLYIQMEYCEK--STLRDTID-QGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKS----VISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYD---------------------------SDILKGNFSIRTAKMQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALF--MDHSGMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGD-LQDLMPTINSLIKQKTGIAQLVKYGLKDLEEVVGLLKKLGIKLQVLINL--GLVYKVQQHNGIIFQ--------FVAFIK--RRQRAVP---------EILAAGGRYDLLIPQFRGPQA--LGPV---PTAIGVSIAIDKISAAVLNMEESVTISSCDLLVVSVGQMSMSRAIN---------------LTQKLWTAGITAEIMYDWSQSQEE 1438

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. UniProt/SwissProt
Match: sp|Q9QZ05|E2AK4_MOUSE (eIF-2-alpha kinase GCN2 OS=Mus musculus OX=10090 GN=Eif2ak4 PE=1 SV=2)

HSP 1 Score: 275.018 bits (702), Expect = 3.770e-73
Identity = 226/717 (31.52%), Postives = 346/717 (48.26%), Query Frame = 1

Query: 2290 YLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVEN---GPVGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEII-FPDE----KHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLK--RFVTRYIESIFVSHGAVEFLAPLLSPK----FINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTD-YDSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRASLHDMVSKDVKNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNR--IHEGYDVINSAFKELKSVEEKLKHFTEDLCFCVNFRPSL-VPLE-TIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEI-YDKDEHGEE 4374
            YL IQME CE   +TL+  ID Q L+ +   +WR  REI+ GL +IH K MIHRDLKP NIFLDS   VKIGD GLA +      E    +   D  + +D      G VGT +Y+ PE+    +S+Y  K D++SLGII FEM + P  T SER  +L  LR P    FPD+    +H K KS    +I  LLNHD   RP+A ELL+S ++PPP+ +E EL + L     N     Y+ M+ ++F +  S    Y                              S I +G+F   T K  + V   I  +F  HGAV+   PLL P+    + + E+  F  +D  G++V LP +LR+ FA ++  N I   KRY I  +++ + + R HP+E +E AFDIVT   +S   TAE +Y +++I ++    +    ++ +N+T L  A++ H  I     D +S+    L    + ++T    +++   K   L   S    L + +K  + +  + +    INS  K+   V + +K+  +DL   V     L V L+ +I  GLV+++       F  +    RR + +          +IL++GG Y   + K + P       V    +AVG SI +  +  ++ ++E+ +     D+ V    +  M+ A+N               L  +LW A I+AEI YD  +  EE
Sbjct:  795 YLYIQMEYCEK--STLRDTID-QGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPDDFDDGEHTKQKS----VISWLLNHDPAKRPTAMELLKSELLPPPQMEESELHEVLHHTLANIDGKAYRTMMSQIFCQHISPAIDYTYD---------------------------SDILKGNFLIRTAKIQQLVCETIVRVFKRHGAVQLCTPLLLPRNRQIYEHNEAALF--MDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAETIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIP---EDKLSQVYVIL----YDAVTEKLTRREVEAKFCNLSLSSN--SLCRLYKFIEQKGDLQDLTPTINSLIKQKTGVAQLVKYSLKDLEDVVGLLKKLGVKLQVSINLGLVYKVQQHTGIIFQFLAFSKRRQRVVP---------EILAAGGRYDLLIPKFRGP-----QTVGPVPTAVGVSIAIDKIFAAVLNMEEPVTVSSCDLLVVSVGQMSMSRAIN---------------LTQKLWTAGITAEIMYDWSQSQEE 1437

HSP 2 Score: 99.7525 bits (247), Expect = 1.932e-19
Identity = 107/420 (25.48%), Postives = 184/420 (43.81%), Query Frame = 1

Query:  703 LVYKGICDGQHRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQ--IRFSDL------KRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKII------NYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKR---------FEELKGRGHSNERTGASTIKKGSKRYDLMKLGVLAKELFFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFLTCEN---LSKNSRQNS------QTVDSAQDLTPWHLFEKS----SRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSS 1833
            +V+KG C G         + Q+    +N      G+ V +++  +++  +      + K+  D     +   E +F+ L KL+HP+++ Y+    +  E  I        VI  +  +  G+                 +L T LS S  +       Y   +L  + YLH  ++    ++  S+L+D +  +KI ++ I KR         FE+ + R  S+      T KKG      + L  L++       P T    +D+P+D  DF+  C         + ++L  H F+  +    L + S ++S      +TV  +  L     F ++    SR   EFE +  LG+GAFG V+KV+N  D   YA+KR+ +N   +   R   EV LLS L+HENIVRY+ +W E  ++ +
Sbjct:  279 MVHKGRCVGS--------DEQLGKVVYNALETATGSFVLLHEWVLQWQKMGPCLTSQEKEKIDKCKRQIQGAETEFSSLVKLSHPNIVRYFAMNSREEEDSI--------VIDILAEHVSGI-----------------SLATHLSHSGPVPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITDYSISKRLADICKEDVFEQARVR-FSDSALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTI--PSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKLPLVEQSPEDSGGQDYIETVIPSNQLPSAAFFSETQKQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPA 662

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. UniProt/SwissProt
Match: sp|D4A7V9|E2AK4_RAT (eIF-2-alpha kinase GCN2 OS=Rattus norvegicus OX=10116 GN=Eif2ak4 PE=3 SV=2)

HSP 1 Score: 274.248 bits (700), Expect = 6.272e-73
Identity = 224/726 (30.85%), Postives = 346/726 (47.66%), Query Frame = 1

Query: 2275 TKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVEN---GPVGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRP-EIIFPDE----KHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLK--RFVTRYIESIFVSHGAVEFLAPLLSPK----FINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTD-YDSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRAS--------LHDMVSKDVKNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNRIHEGYDVINSAFKELKSVEEKLKHFTEDLCFCVNFRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEI-YDKDEHGEE 4374
            T+   YL IQME CE   +TL+  ID Q L+ +   +WR  REI+ GL +IH K MIHRDLKP NIFLDS   VKIGD GLA +      E    +   D+ + +D      G VGT +Y+ PE+    +S+Y  K D++SLGII+FEM + P  T SER  +L  LR P    FPD+    +H K KS    +I  LLNHD   RP+A ELL+S ++PPP+ +E EL + L     N+    Y+ M+ +LF +  S    Y                              S I +G+F   T K  + V   I  +F  HGAV+   PLL P+    + + E+  F  +D  G++V LP +LR+ FA ++  N I   KRY I  +++ + + R HP+E +E AFDIVT   +S   TAE +Y ++++ ++    +    ++ +N+T L  A++ H  I           L+D V++ +   + +      +L+      +   +   G  L+DL         +      ++  + K+L+ V   LK     L   +N    LV     ++G++FQ        FL   K  RR + +          +IL++GG Y   + K + P     G V    +AVG SI +  +  ++ ++ + +     D+ V  + +  M+ A+N               L  +LW A I+AEI YD  +  EE
Sbjct:  791 TEAVHYLYIQMEYCEK--STLRDTID-QGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFNAEGKQDDQAGDHVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIILFEMSYHPMVTASERIFVLNQLRDPTSPKFPDDFEDGEHTKQKS----VISWLLNHDPAKRPTAMELLKSELLPPPQMEESELHEVLHHTLANTDGKAYRTMMSQLFCQHSSPAIDYT---------------------------YDSDILKGNFLIRTAKIQQLVCETIVRVFKRHGAVQLCTPLLLPRNRQIYEHNEAALF--MDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTANSSLPTAETIYTIYEVIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGD-LQDLTPTINSLIKQKTGIAQLVKYSLKDLEEVVGLLKKLGVKLQVSINL--GLVYKVQQHNGIIFQ--------FLAFSK--RRQRVVP---------EILAAGGRYDLLIPKFRGP--QALGPV---PTAVGVSIAIDKIFAAVLNMGEPVTVSSCDLLVVSAGQMSMSRAIN---------------LTQKLWTAGITAEIMYDWSQSQEE 1438

HSP 2 Score: 118.627 bits (296), Expect = 3.336e-25
Identity = 147/671 (21.91%), Postives = 272/671 (40.54%), Query Frame = 1

Query:  133 EVKALRSIFGKEFKLIRPKNLKKKYEPPLVSVLI---NVDSSNLNSALSLRISFDCPQNYPFDIPKISIYDAVNVPTNLVEQLLIILNEKSNLLKGYSMIYNLVEEARCIIIKHFETDHKSVNS-ILTINKVLNEDEIMQTETKQSEFLKNLLRQRRDS-------------ARXXXXXXXXXXXCDHSYKTDELEFE--------------------HCSDRSSGCLVYKGICDGQ---------------------HRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQIRFSDLKR---------KQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKR---------FEELKGRGHSNERTGASTIKKGSKRYDLMKLGVLAK--------ELFFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFLTCEN---LSKNSRQNSQTVDSAQDLTPWHLFEKS----------SRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSS 1833
            E++AL +I+G +F+ +RP    +  EPP +++++    +    +   + LR+   CP  YP  +P+I + +   +    V  L   L E +    G  MI+ L    +  + +H +   KS +  +L       +  +++   K+ +  + +L + +               A+ E+         DH+ K D                         H S RS     Y  +C G+                     H+ K +  + Q+    +N      G+ V +Y+      K+         K+  D     +   E +F+ L KL+HP+++ Y+    +  +  I        V+  +  +  G+ L           +   +    + + ++  Y   +L  + YLH  ++    ++  S+L+D +  +KI ++ I KR         FE+ + R  S+      T KKG    D+ +LG+L          E +   IP      +D+P+D  DF+  C         + ++L  H F+  +    L + S ++S   D  + + P +    +          SR   EFE +  LG+GAFG V+KV+N  D   YA+KR+ +N   ++  R   EV LLS L+HENIVRY+ +W E  ++ +
Sbjct:   26 ELQALEAIYGSDFQDLRPDARGRVREPPEINLVLYPQGLAGEEVYVQVELRVK--CPPTYPDVVPEIELKNTKGLSNESVNLLKSHLEELAKKQCGEVMIFELAHHVQSFLSEHNKPPPKSFHEEMLERQAQEKQQRLLEARQKEEQEQREILHEIQKRKEEIKEEKKRKEMAKQERLEITSLTNQDHASKRDPAGHRAAAFLHGGSPDFVGNGKARAHSSGRSRRERQY-SVCSGEASPGSCDILYFCVGSADQLMVHKGKCVGSDEQLGKVVYNALETATGSFVLLYEWVLQWQKKMGPCLTSQEKEKIDKCKKQIQGAETEFSSLVKLSHPNIVRYFAMNSREEKDSI--------VVDILAEHISGISL-----------AAHLSHSGPVPMHQLRKYTAQLLAGLDYLHRNSVVHKVLSTASVLVDAEGTVKITDYSISKRLADICKEDVFEQTRVR-FSDSALPYKTGKKG----DVWRLGLLLLSLSQGQECEEYPVTIP------SDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKMPLVEQSPEDSGGQDYIETIIPSNQLPSAAFFTETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPA 663

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. UniProt/SwissProt
Match: sp|Q9HGN1|GCN2_SCHPO (eIF-2-alpha kinase GCN2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gcn2 PE=2 SV=2)

HSP 1 Score: 226.098 bits (575), Expect = 4.775e-58
Identity = 206/691 (29.81%), Postives = 330/691 (47.76%), Query Frame = 1

Query: 1579 LFEKS-SRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSSAI---SVIKPETTDSLLLDFQDKKNSIMFSANVNFSQIEHCSRHFDLESFXXXXXXXXXXXXVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPFHGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEML---NQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKL--YSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPKF--INTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDYDSPNK-TAEILYLLHQIFKKSFNSEHATLT---LEINNTNLFYALMNHYSI 3606
            L+ KS SR   +FE +  LG G FG+VVKV+N  D ++YA+K++ L +  K+ +R ++EV  LS L+HE++VRY+ +W  E + + + T  +  + +S S   N +      S +  +   SL + F+D  NS   SA+                                  ++ P     + +ISF YS+  D   +    K++  E +  VK+           Q N  +         L IQME CE    +LQ  I D K+ ++E  +WR  R+I+  L +IHS+ M+HRDLKP NIFLD  + VK+GD GLA    N+  +  N ++        D+  G VGT +Y+ PE+L   N   Y  K DMYSLGII+FEMC   F T  ER  I+  +R P I FP    F   S   ++I  LL HD   RPS+ ELL+S  IPP  G+E       + +  N ++  Y  +LK LF ++    +   Y+ +L  +S     +S+      R   + L+                V  ++  +F  HGA E  + +L PK    + +    SLLD  G ++ LP +  + +A  +  N +   K Y I  ++ ++A     P+   E +FDI T+ D+ +   AE +  L ++      +E  +LT   + IN+ ++  +++++  +
Sbjct:  544 LYRKSVSRYETDFEELEFLGRGGFGEVVKVKNRIDGRFYAVKKLVLLSDDKENSRILREVMTLSRLHHEHVVRYYTAWV-ETEANDTVTEIISSDSESLSQSLNMAVDFRQSSSLPADKLSSLDIHFEDDYNS---SAD----------------------------------EEDPE----ASDISFQYSNTSDK--EGSSDKDSSIEEASSVKT-----------QENGLNAT-------LYIQMEYCEKL--SLQDIIRD-KIPVDE--MWRLFRQILEALAYIHSRGMMHRDLKPGNIFLDENRNVKLGDFGLAT--ENENYQDNNDKWKNRQSADEDLTTG-VGTALYVAPELLSRRNGVRYDAKVDMYSLGIILFEMCMT-FSTSMERIRIIDTIRSPSISFPSTFPFSRASHEFKVIHCLLQHDPTKRPSSQELLESEAIPPKVGEEFIQEG--LRLLSNPNTPYYLKLLKVLFGQVPDRHKDFTYDFNLSEESGVLSKVSD------RGWDSLLAC--------------LVRDHVVKVFRRHGAKERESHILFPKSSQYDKDQASVSLLDKNGTLLQLPYDTVLPYARNVARNAVEEEKTYLISDVF-REAKGGGRPKAIKEISFDITTNSDNLDWYDAETIKALDEVL-----TEIPSLTESCILINHADILSSILDYLQV 1135

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. UniProt/SwissProt
Match: sp|P15442|GCN2_YEAST (eIF-2-alpha kinase GCN2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=GCN2 PE=1 SV=3)

HSP 1 Score: 174.096 bits (440), Expect = 3.804e-42
Identity = 193/710 (27.18%), Postives = 312/710 (43.94%), Query Frame = 1

Query: 2293 LMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVT----ESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSS-YTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFP---DEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLA-PLLSPKF-INTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRD--HPRERIEAAFDIVTDYDSPNK--TAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRASLHDMVSKDVKNLDSDHFRSLTNLTDKQKA--NIKSILLFNGSRLEDLEKHFKIFQNRIHEGYDVINSAFKELKSVEEKLKHFTEDLCFCVNFRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAV-------NVVVQ--YDIEMDGIIWDLVSRLWAANISAEI 4347
            L IQME CE +  TL   I  + L       WR  R+I+  L +IHS+ +IHRDLKP NIF+D  + VKIGD GLA N +  +     +S N     DN  S       +GT MY+  E+L+ +  Y  K DMYSLGII FEM + PF TG ER +IL  LR   I FP   D+   K++   + L   L++HD   RP A  LL S  +P     E+                     +KE    L +    ++   + +S FN   S T  ++F N   P S+       F   L+  +T  +  IF  HG +E  A P + PK  I      + +LD  G V+ L  +L    A +L  N     K+Y +  +Y+     R    PR+  E  FDI++   S +    AE L ++ +I       E       +N+ ++  ++ N  +I  +   +VS+ +  +     RS   + ++ KA  NI S  L N   L D    F+  + R+++   +I+S    LK +E+ L H ++ L +       L PLE +   +V   +      F       +             +  ++++GG Y + +    RP   +  +  +   AVG ++      ++++ I Q          + +   +L ++AV       +V++    +  +D I   +++ LW  NI A++
Sbjct:  784 LFIQMEYCENR--TLYDLIHSENLNQQRDEYWRLFRQILEALSYIHSQGIIHRDLKPMNIFIDESRNVKIGDFGLAKNVHRSLDILKLDSQNLPGSSDNLTS------AIGTAMYVATEVLDGTGHYNEKIDMYSLGIIFFEMIY-PFSTGMERVNILKKLRSVSIEFPPDFDDNKMKVEKKIIRL---LIDHDPNKRPGARTLLNSGWLPVKHQDEV---------------------IKEALKSLSNPSSPWQ-QQVRESLFNQSYSLTNDILFDN-SVPTSTP------FANILRSQMTEEVVKIFRKHGGIENNAPPRIFPKAPIYGTQNVYEVLDKGGTVLQLQYDLTYPMARYLSKNPSLISKQYRMQHVYRPPDHSRSSLEPRKFGEIDFDIISKSSSESGFYDAESLKIIDEILTVFPVFEKTNTFFILNHADILESVFNFTNIDKAQRPLVSRMLSQV--GFARSFKEVKNELKAQLNISSTAL-NDLELFDFRLDFEAAKKRLYKL--MIDSP--HLKKIEDSLSHISKVLSY-------LKPLE-VARNVVISPLSNYNSAFY------KGGIMFHAVYDDGSSRNMIAAGGRYDTLISFFARPSGKKSSNTRK---AVGFNL----AWETIFGIAQNYFKLASGNRIKKRNRFLKDTAVDWKPSRCDVLISSFSNSLLDTIGVTILNTLWKQNIKADM 1424

HSP 2 Score: 82.8037 bits (203), Expect = 3.027e-14
Identity = 39/75 (52.00%), Postives = 54/75 (72.00%), Query Frame = 1

Query: 1594 SRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEE 1818
            SR   +FE I  LG+GAFG VVK RN  D++YYAIK++R    ++K +  + EV LL+SLNH+ +VRY+A+W EE
Sbjct:  593 SRYASDFEEIAVLGQGAFGQVVKARNALDSRYYAIKKIR--HTEEKLSTILSEVMLLASLNHQYVVRYYAAWLEE 665

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. TrEMBL
Match: A0A3Q2X7J9 (Eukaryotic translation initiation factor 2 alpha kinase 4 OS=Haplochromis burtoni OX=8153 GN=EIF2AK4 PE=4 SV=1)

HSP 1 Score: 391.734 bits (1005), Expect = 3.620e-108
Identity = 405/1500 (27.00%), Postives = 675/1500 (45.00%), Query Frame = 1

Query:  109 DVTILQSDEVKALRSIFGKEFKLIRPKNLKKKYEPPLVSVLINVDSSNLNSALSLRISFD----CPQNYPFDIPKISIYDAVNVPTNLVEQLLIILNEKSNLLKGYSMIYNLVEEARCIIIKHFETDHKSVNSILTINK-------VLNEDEIMQTETKQSEFL-KNLLRQRRDSARXXXXXXXXXXXCDHSYKTDELE--FEHCSDRSSGCLVYKGICDGQHRIKTINPNMQIKL--NTFNGFNRQKGTNVKIYQIR----------FSDLKRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKRFEEL-------KGRGHSNERTGASTIKKGSKRYDLMKLGVL------AKEL--FFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFL---TCENLSKNSRQNSQ--TVDSAQDLTPW-HLF----------EKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSS----SETNNLQP--NIDSSSIRNNTSSAISVIKPETTDSLLL--DFQDKKNSIMFSANVNFS-QIEHCS--RHFDLESFXXXXXXXXXXXXVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPFHGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPKFINTESG--FFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDY-DSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRASLHDMVSKDVKNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNRIHEGYDVINSAFKELKSVEEKLKHFTEDLCFCVNFRPSL-VPLETIYH-GLVFQII--LKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEI-YD 4353
            D  + Q +E++AL SIF  +FK +R  +  K   PP V V +    + LN+     ++ D    CP  YP   P++ + +A  +  + ++ L   LN+ +    G  MIY L +  +  + +H +   +S +  +  N+        L E + M    KQ E + K ++ + +     +++ +++ E     +  D L       + R  G      +CDG+   +    ++  +   N + GF    G    IY+            F+  +R + E+ K   +   E +F  L +L HP+L+ Y G      E+ I       +++++V  N     L       T  G         + + K+ +Y   +L A+ YLH  ++   ++   S+L+D +  +++ ++ + KRF ++       + R   +E T   T  +  K+ D+  LG++       KE+  +   +P      + +P+D  DF+  C           ++L  H FL   + ++LS+    + +   VD A  + P  H+            + SR   EFE +  LG+GAFG V+KV+N  D  YYA+KR+++N   K+  R   EV LLS LNHENIVRY+ +W E  +  +    S T++ +P  + D +  R N         PE  + L L  D +D       S++V +S  IE  S  R    +S  D D D D +  V      P       +I F   DN D  +D         E+SK  +S R                       YL IQME CE   +TL+  ID Q L+ ++  +WR  REI+ GL +IH + MIHRDLKP NIFLDSQ  VKIGD GLA    +    S +    K +   N    G VGT +Y+ PE+    +++Y  K D++SLGII+FEM ++P  TG+ER  +L  LR   IIFP++   + + +   +I+ LL HD  LRP+A ELL+S ++PPP+ +E EL + L     N +   Y+ M+ +LF++  S +  Y                                      +   L +++  Y         AV    PL  P+      G      +D  G++V LP +LR+ FA F+  N ++  KRY+I  +++ + + R HPRE +E AFDI+T   +S    AEI+Y + +I ++    +     + +N+T+LF A++ H  +        S  + +L S+       LT ++          + + L+ L K+ +   N + +   +++S  K+  +V +  K   +DL       P L V L  + + GLV+++     V  +F+  I+  RR      TV       IL++GG Y   +++ + P     G V    SAVGAS+ V  +  ++ S+E+       D+ V       M+ A+ V               V +LW+  I+A+I YD
Sbjct:   18 DYAVQQENELEALASIFEDDFKDLRSNDPWKVKRPPEVYVCLR--PNGLNNGQECYVTVDLHVKCPPTYPDVPPELELKNAKGLSNDNLKNLQAELNKLAAEQCGEVMIYQLADHIQGFLSEHNKPPSRSFHEEMLKNQRRQQEKLALEEQQRMDQRRKQEEEMEKEIMAEIQRREEEKREEKRRKEMAKQVFSLDSLNGFLFAGATREHGAF---DVCDGEESTQPRRRSVSERFGRNIYCGFEVNSGDFAVIYEWSLQWNKKMSKFFTTQERGRIENCKKQ-IHAAENEFNSLLRLEHPNLVQYLGLSVVEKEECIV----VYMLVEHVAGNSLNQSL-------TAHGP--------VPLDKLCHYTAQLLTALDYLHSNSVVHKQLGPSSVLVDSEGNIRVTDYSLSKRFADICKEDIFEQARVRFSEETAMPT--RTGKKGDVWNLGLMLLALSQGKEVKEYPVTVP------SSLPADFQDFLQKCLCLNDTERWTAQQLLDHSFLKPSSPKHLSQYQDNSPEDLAVDFASSVIPRSHILNAPFSSVLQHRQISRFVSEFEELQLLGKGAFGAVIKVQNKLDGCYYAVKRIQVNPASKQFRRIKGEVTLLSRLNHENIVRYYNAWIERHELPATGVLSNTDSSEPLSSADKAPQRKNA--------PERLNELGLPDDVEDIAPPPALSSSVEWSTSIERSSSARCSGRQSSDDEDDDDDDEEDVFGASFLPSNNDSRSDIIF---DNGDESMD---------EMSKSTESDR-----------------HLITAHYLYIQMEYCEK--STLRDTID-QGLHQDQNRLWRLFREILDGLAYIHEQGMIHRDLKPVNIFLDSQDHVKIGDFGLA---TDHPANSGSAVMAKPDPTGN--MTGMVGTALYVSPEVQGNTKATYNQKVDLFSLGIILFEMSYRPMTTGAERIAVLSQLRGEPIIFPEDFTLREQGTQKRVIEWLLKHDPALRPTALELLKSELLPPPQMEESELHEVLQHTMANINGKSYRTMVAQLFAQNTSPVMDYA-------------------------------------YDIDLYKWILFYC--------AVRLQTPLFLPRNRKLYDGIELACFMDHSGMLVTLPYDLRIAFARFVARNNVTHLKRYSIERVFRPKKLDRAHPRELLECAFDIITPVSNSLLPEAEIIYTISEIIQEFPVLQERNYNIYLNHTSLFKAVLLHSGVPEDKLSQASNILCDLMSE------KLTKREAEAKFCNFSLSANCLQTLYKYIEQKGN-LQDLVPLLSSLTKQKTAVTQLAKQGLKDLEELTVLLPKLGVKLMVVINLGLVYKVQHHSGVIFQFVAFIRKRRR------TVP-----DILAAGGRYDHLILEFRGPV--STGPVP---SAVGASVAVDKVCAAIVSMEEPPTVSSYDVLVVPVGHSSMSKAIRV---------------VQKLWSTGIAADINYD 1356

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. TrEMBL
Match: A0A3Q2X7G1 (Eukaryotic translation initiation factor 2 alpha kinase 4 OS=Haplochromis burtoni OX=8153 GN=EIF2AK4 PE=4 SV=1)

HSP 1 Score: 390.193 bits (1001), Expect = 5.369e-108
Identity = 369/1284 (28.74%), Postives = 598/1284 (46.57%), Query Frame = 1

Query:  733 HRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQIR----------FSDLKRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKRFEEL-------KGRGHSNERTGASTIKKGSKRYDLMKLGVL------AKEL--FFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFL---TCENLSKNSRQNSQ--TVDSAQDLTPW-HLF----------EKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSS----SETNNLQP--NIDSSSIRNNTSSAISVIKPETTDSLLL--DFQDKKNSIMFSANVNFS-QIEHCS--RHFDLESFXXXXXXXXXXXXVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPFHGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVT-------ESANQEYVKDNGLSNDVENGPVGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSL-LYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPKFINTESG--FFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDY-DSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRASLHDMVSKDVKNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNRIHEGYDVINSAFKELKSVEEKLKHFTEDLCFCVNFRPSL-VPLETIYH-GLVFQII--LKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEI-YD 4353
            H+ K++  + +   N + GF    G    IY+            F+  +R + E+ K   +   E +F  L +L HP+L+ Y G      E+ I       +++++V  N     L       T  G         + + K+ +Y   +L A+ YLH  ++   ++   S+L+D +  +++ ++ + KRF ++       + R   +E T   T  +  K+ D+  LG++       KE+  +   +P      + +P+D  DF+  C           ++L  H FL   + ++LS+    + +   VD A  + P  H+            + SR   EFE +  LG+GAFG V+KV+N  D  YYA+KR+++N   K+  R   EV LLS LNHENIVRY+ +W E  +  +    S T++ +P  + D +  R N         PE  + L L  D +D       S++V +S  IE  S  R    +S  D D D D +  V      P       +I F   DN D  +D         E+SK   S R  S     ++++           YL IQME CE   +TL+  ID Q L+ ++  +WR  REI+ GL +IH + MIHRDLKP NIFLDSQ  VKIGD GLA +    V        ES +    K +   N    G VGT +Y+ PE+    +++Y  K D++SLGII+FEM ++P  TG+ER  +L  LR   IIFP++   + + +   +I+ LL HD  LRP+A ELL+S ++PPP+ +E EL + L     N +   Y+ M+ +LF++  S +  Y   + LY+  F+          F N +                L++ V   I  IF  HGAV    PL  P+      G      +D  G++V LP +LR+ FA F+  N ++  KRY+I  +++ + + R HPRE +E AFDI+T   +S    AEI+Y + +I ++    +     + +N+T+LF A++ H  +        S  + +L S+       LT ++          + + L+ L K+ +   N + +   +++S  K+  +V +  K   +DL       P L V L  + + GLV+++     V  +F+  I+  RR      TV       IL++GG Y   +++ + P     G V    SAVGAS+ V  +  ++ S+E+       D+ V       M+ A+ V               V +LW+  I+A+I YD
Sbjct:   99 HQGKSLGVSERFGRNIYCGFEVNSGDFAVIYEWSLQWNKKMSKFFTTQERGRIENCKKQ-IHAAENEFNSLLRLEHPNLVQYLGLSVVEKEECIV----VYMLVEHVAGNSLNQSL-------TAHGP--------VPLDKLCHYTAQLLTALDYLHSNSVVHKQLGPSSVLVDSEGNIRVTDYSLSKRFADICKEDIFEQARVRFSEETAMPT--RTGKKGDVWNLGLMLLALSQGKEVKEYPVTVP------SSLPADFQDFLQKCLCLNDTERWTAQQLLDHSFLKPSSPKHLSQYQDNSPEDLAVDFASSVIPRSHILNAPFSSVLQHRQISRFVSEFEELQLLGKGAFGAVIKVQNKLDGCYYAVKRIQVNPASKQFRRIKGEVTLLSRLNHENIVRYYNAWIERHELPATGVLSNTDSSEPLSSADKAPQRKNA--------PERLNELGLPDDVEDIAPPPALSSSVEWSTSIERSSSARCSGRQSSDDEDDDDDDEEDVFGASFLPSNNDSRSDIIF---DNGDESMD---------EMSKVEPSKRPASDTTESTESD----RHLITAHYLYIQMEYCEK--STLRDTID-QGLHQDQNRLWRLFREILDGLAYIHEQGMIHRDLKPVNIFLDSQDHVKIGDFGLATDHPANVAAGKFEVEESGSAVMAKPDPTGN--MTGMVGTALYVSPEVQGNTKATYNQKVDLFSLGIILFEMSYRPMTTGAERIAVLSQLRGEPIIFPEDFTLREQGTQKRVIEWLLKHDPALRPTALELLKSELLPPPQMEESELHEVLQHTMANINGKSYRTMVAQLFAQNTSPVMDYAYDIDLYKGSFS----------FNNAK----------------LQQHVYESITRIFKKHGAVRLQTPLFLPRNRKLYDGIELACFMDHSGMLVTLPYDLRIAFARFVARNNVTHLKRYSIERVFRPKKLDRAHPRELLECAFDIITPVSNSLLPEAEIIYTISEIIQEFPVLQERNYNIYLNHTSLFKAVLLHSGVPEDKLSQASNILCDLMSE------KLTKREAEAKFCNFSLSANCLQTLYKYIEQKGN-LQDLVPLLSSLTKQKTAVTQLAKQGLKDLEELTVLLPKLGVKLMVVINLGLVYKVQHHSGVIFQFVAFIRKRRR------TVP-----DILAAGGRYDHLILEFRGPV--STGPVP---SAVGASVAVDKVCAAIVSMEEPPTVSSYDVLVVPVGHSSMSKAIRV---------------VQKLWSTGIAADINYD 1261

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. TrEMBL
Match: A0A3Q3C620 (Eukaryotic translation initiation factor 2 alpha kinase 4 OS=Haplochromis burtoni OX=8153 GN=EIF2AK4 PE=4 SV=1)

HSP 1 Score: 389.423 bits (999), Expect = 5.220e-107
Identity = 369/1284 (28.74%), Postives = 598/1284 (46.57%), Query Frame = 1

Query:  733 HRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQIR----------FSDLKRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKRFEEL-------KGRGHSNERTGASTIKKGSKRYDLMKLGVL------AKEL--FFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFL---TCENLSKNSRQNSQ--TVDSAQDLTPW-HLF----------EKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSS----SETNNLQP--NIDSSSIRNNTSSAISVIKPETTDSLLL--DFQDKKNSIMFSANVNFS-QIEHCS--RHFDLESFXXXXXXXXXXXXVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPFHGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVT-------ESANQEYVKDNGLSNDVENGPVGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSL-LYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPKFINTESG--FFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDY-DSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRASLHDMVSKDVKNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNRIHEGYDVINSAFKELKSVEEKLKHFTEDLCFCVNFRPSL-VPLETIYH-GLVFQII--LKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEI-YD 4353
            H+ K++  + +   N + GF    G    IY+            F+  +R + E+ K   +   E +F  L +L HP+L+ Y G      E+ I       +++++V  N     L       T  G         + + K+ +Y   +L A+ YLH  ++   ++   S+L+D +  +++ ++ + KRF ++       + R   +E T   T  +  K+ D+  LG++       KE+  +   +P      + +P+D  DF+  C           ++L  H FL   + ++LS+    + +   VD A  + P  H+            + SR   EFE +  LG+GAFG V+KV+N  D  YYA+KR+++N   K+  R   EV LLS LNHENIVRY+ +W E  +  +    S T++ +P  + D +  R N         PE  + L L  D +D       S++V +S  IE  S  R    +S  D D D D +  V      P       +I F   DN D  +D         E+SK   S R  S     ++++           YL IQME CE   +TL+  ID Q L+ ++  +WR  REI+ GL +IH + MIHRDLKP NIFLDSQ  VKIGD GLA +    V        ES +    K +   N    G VGT +Y+ PE+    +++Y  K D++SLGII+FEM ++P  TG+ER  +L  LR   IIFP++   + + +   +I+ LL HD  LRP+A ELL+S ++PPP+ +E EL + L     N +   Y+ M+ +LF++  S +  Y   + LY+  F+          F N +                L++ V   I  IF  HGAV    PL  P+      G      +D  G++V LP +LR+ FA F+  N ++  KRY+I  +++ + + R HPRE +E AFDI+T   +S    AEI+Y + +I ++    +     + +N+T+LF A++ H  +        S  + +L S+       LT ++          + + L+ L K+ +   N + +   +++S  K+  +V +  K   +DL       P L V L  + + GLV+++     V  +F+  I+  RR      TV       IL++GG Y   +++ + P     G V    SAVGAS+ V  +  ++ S+E+       D+ V       M+ A+ V               V +LW+  I+A+I YD
Sbjct:  277 HQGKSLGVSERFGRNIYCGFEVNSGDFAVIYEWSLQWNKKMSKFFTTQERGRIENCKKQ-IHAAENEFNSLLRLEHPNLVQYLGLSVVEKEECIV----VYMLVEHVAGNSLNQSL-------TAHGP--------VPLDKLCHYTAQLLTALDYLHSNSVVHKQLGPSSVLVDSEGNIRVTDYSLSKRFADICKEDIFEQARVRFSEETAMPT--RTGKKGDVWNLGLMLLALSQGKEVKEYPVTVP------SSLPADFQDFLQKCLCLNDTERWTAQQLLDHSFLKPSSPKHLSQYQDNSPEDLAVDFASSVIPRSHILNAPFSSVLQHRQISRFVSEFEELQLLGKGAFGAVIKVQNKLDGCYYAVKRIQVNPASKQFRRIKGEVTLLSRLNHENIVRYYNAWIERHELPATGVLSNTDSSEPLSSADKAPQRKNA--------PERLNELGLPDDVEDIAPPPALSSSVEWSTSIERSSSARCSGRQSSDDEDDDDDDEEDVFGASFLPSNNDSRSDIIF---DNGDESMD---------EMSKVEPSKRPASDTTESTESD----RHLITAHYLYIQMEYCEK--STLRDTID-QGLHQDQNRLWRLFREILDGLAYIHEQGMIHRDLKPVNIFLDSQDHVKIGDFGLATDHPANVAAGKFEVEESGSAVMAKPDPTGN--MTGMVGTALYVSPEVQGNTKATYNQKVDLFSLGIILFEMSYRPMTTGAERIAVLSQLRGEPIIFPEDFTLREQGTQKRVIEWLLKHDPALRPTALELLKSELLPPPQMEESELHEVLQHTMANINGKSYRTMVAQLFAQNTSPVMDYAYDIDLYKGSFS----------FNNAK----------------LQQHVYESITRIFKKHGAVRLQTPLFLPRNRKLYDGIELACFMDHSGMLVTLPYDLRIAFARFVARNNVTHLKRYSIERVFRPKKLDRAHPRELLECAFDIITPVSNSLLPEAEIIYTISEIIQEFPVLQERNYNIYLNHTSLFKAVLLHSGVPEDKLSQASNILCDLMSE------KLTKREAEAKFCNFSLSANCLQTLYKYIEQKGN-LQDLVPLLSSLTKQKTAVTQLAKQGLKDLEELTVLLPKLGVKLMVVINLGLVYKVQHHSGVIFQFVAFIRKRRR------TVP-----DILAAGGRYDHLILEFRGPV--STGPV---PSAVGASVAVDKVCAAIVSMEEPPTVSSYDVLVVPVGHSSMSKAIRV---------------VQKLWSTGIAADINYD 1439

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. TrEMBL
Match: A0A2I4BIC2 (eukaryotic translation initiation factor 2-alpha kinase 4 OS=Austrofundulus limnaeus OX=52670 GN=eif2ak4 PE=4 SV=1)

HSP 1 Score: 388.652 bits (997), Expect = 8.872e-107
Identity = 362/1292 (28.02%), Postives = 591/1292 (45.74%), Query Frame = 1

Query:  733 HRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQIR----------FSDLKRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKRFEEL-------KGRGHSNERTGASTIKKGSKRYDLMKLGVL------AKEL--FFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFLTCENLSKNSRQNSQT------VDSAQDLTPWH----------LFEKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSSAISVI-KPETTDSLLL--DFQDKK------NSIMFSANVNFSQIEHCSRHFDLESFXXXXXXXXXXXXVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPFHGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVT-------ESANQEYVKDNGLSNDVENGPVGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSL-LYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPK----FINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDY-DSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRASLHDMVSKDVKNLDSDHFRSLTN--LTDKQKANIKSILLFNGSRLEDLEKHFKIFQNRIHEGYDVINSAFKELKSVEEKLKHFTEDL-CFCVNFRPSLVPLETIYH-GLVFQII--LKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEI-YDKDEHGE 4371
            H+ K++  + ++  N + GF    G    IY+            F+  ++ + E+ K   +   E +F  L +L+HP+L+ Y        E  +      DL++++V   +    L                 ++ LS+ K+ +Y   +L A+ YLH  ++   ++   S+L+D +  +++ ++ + KRF E+       + R   +E T   T  K  K+ D+  LG++       KE+  +   +P      + +P+D  DF+  C           ++L  H FL   +  KN  Q+ +T      VD A  + P            +  + SR   EFE +  LG+GAFG V+KV+N  D  YYA+KR+++N   K+  R   EV LLS LNHENIVRY+ +W E  +  S+    +  N DSS   +     +     P+  + L L  D +D        +S+ +S ++  S    C  H   E   D D + + +  V      P   +   +I F   DN D         +  DE+S+   S R  +     S ++        I  YL IQME CE   +TL+  I DQ LY+++  +WR  REI+ GL +IH + MIHRDLKP NIFLDSQ  VKIGD GLA +    V        E+      K +   N    G VGT +Y+ PE+    +++Y  K D++SLGII+FEM ++PF TG+ER  +L  LR   I FP++     +    ++I+ LL HD  LRP+  ELL+S ++PPP+ +E EL + L     N +   Y+ M+ +LF++  S +  Y   + L++  F+                          F    L++ V   I  IF  HGAV    PLL P+    +  +E   F  +D  G++V LP +LRM FA F+  N ++  KRY I  +++ + + R HPRE +E A+DI+T   +S    AE +Y + +I ++    +     + +N+T+L  A++        LH  V +D  N  S+      N  LT ++          + + L+ L ++    +  + E   ++ S  K+  +V +  K   +DL    V  R   V L  + + GLV+++     V  +F+  I+     K  RT         I+++GG Y   +++ + P     G +    SAVGASI +  +  +M  +E+       D  V       M+ A+NV               V +LW+  +SA+I YD  +  E
Sbjct:  275 HKGKSLGESKRLGRNVYCGFEANSGEFSVIYEWSLRWSQKMGKFFTSQEKGKIENCKKQ-IHAAENEFNSLLRLDHPNLVRYMALSSSEKEDSLV----VDLLVEHVAGVNLNQSLIS---------------QSPLSLDKLGHYAAQLLSALNYLHSNSVVHKQLGASSVLVDSEGNVRLTDYSLSKRFAEICKEDIFEQARVRFSEDTAMPT--KTGKKGDVWNLGLMLLALSQGKEVKEYPVTVP------SSLPADFQDFLHKCLCLNDAERWTAQQLLDHAFLKPPS-PKNLPQDQETSPEDLAVDFASSVIPRSSILHAPFTSGMQRQFSRYFDEFEELQLLGKGAFGAVIKVKNNLDGCYYAVKRIQVNPASKQFRRIKGEVTLLSRLNHENIVRYYNAWIERHEVPST---GVLSNTDSSEPPSAAGKHLGCGNPPQRLNELGLPDDVEDVAPPPALSSSVEWSTSIERSSSAKCGGHQSSEEDDDDDDEDEDEDDVFGASFLPSDSESRSDIIF---DNGD---------DGTDEMSQVEPSKRPATDTTESSDSDRL----LSIAHYLYIQMEYCEK--STLRDTI-DQSLYLDQNRLWRLFREILDGLAYIHEQGMIHRDLKPVNIFLDSQDHVKIGDFGLATDHPANVAAGKFDLEENITALTPKQDPAGN--MTGMVGTALYVSPEVQGNTKATYNQKVDLFSLGIILFEMSYRPFTTGAERISVLSQLRMESITFPEDFLPHEQGKQRKVIEWLLKHDPALRPTTQELLKSELLPPPQMEESELHEVLQHTMANVNGKAYRTMVGQLFAQNTSPVMDYTYDIDLHKGSFS--------------------------FTGAKLQQHVYETISRIFKKHGAVRLHTPLLLPRNRKLYDGSELACF--MDHSGMLVTLPYDLRMAFARFVARNNVTQLKRYCIERVFRPRKLDRAHPRELVECAYDIITPITNSLLPDAETIYTISEIVQEFPALQERNYNIYLNHTSLLKAIL--------LHSGVPEDKLNQASNILCDAMNEKLTRREVEAKFFNFSLSSTSLQTLYRYIDNGKGTLQELGPLLTSLTKQKTAVTQLAKQGAKDLEELTVLLRKLGVKLRVVVNLGLVYKVQHHSGVIFQFVAFIR-----KRKRT------VPDIVAAGGRYDHLILEFRGP--APTGPI---PSAVGASIALDKICAAMAGMEEPPTVSTCDALVVPVGHSSMSRAINV---------------VQKLWSTGVSADIAYDVSQSQE 1446

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. TrEMBL
Match: A0A3P8NFA2 (Eukaryotic translation initiation factor 2 alpha kinase 4 OS=Astatotilapia calliptera OX=8154 GN=EIF2AK4 PE=4 SV=1)

HSP 1 Score: 385.956 bits (990), Expect = 1.426e-106
Identity = 369/1285 (28.72%), Postives = 598/1285 (46.54%), Query Frame = 1

Query:  733 HRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQIR----------FSDLKRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKRFEEL-------KGRGHSNERTGASTIKKGSKRYDLMKLGVL------AKEL--FFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFL---TCENLSKNSRQNSQ--TVDSAQDLTPW-HLF----------EKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSS----SETNNLQP--NIDSSSIRNNTSSAISVIKPETTDSLLL--DFQDKKNSIMFSANVNFS-QIEHCS--RHFDLESFXXXXXXXXXXXXVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPFHGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVT-------ESANQEYVKDNGLSNDVENGPVGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSL-LYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPKFINTESG--FFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDY-DSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRASLHDMVSKDVKNLDSDHFRSLTNLTDKQKANIKSI-LLFNGSRLEDLEKHFKIFQNRIHEGYDVINSAFKELKSVEEKLKHFTEDLCFCVNFRPSL-VPLETIYH-GLVFQII--LKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEI-YD 4353
            H+ K++  + +   N + GF    G    IY+            F+  +R + E+ K   +   E +F  L +L HP+L+ Y G      E+ I       +++++V  N     L       T  G         + + K+ +Y   +L A+ YLH  ++   ++   S+L+D +  +++ ++ + KRF ++       + R   +E T   T  +  K+ D+  LG++       KE+  +   +P      + +P+D  DF+  C           ++L  H FL   + ++LS+    + +   VD A  + P  H+            + SR   EFE +  LG+GAFG V+KV+N  D  YYA+KR+++N   K+  R   EV LLS LNHENIVRY+ +W E  +  +    S T++ +P  + D +  R N         PE  + L L  D +D       S++V +S  IE  S  R    +S  D D D D +  V      P       +I F   DN D  +D         E+SK   S R  S     ++++           YL IQME CE   +TL+  ID Q L+ ++  +WR  REI+ GL +IH + MIHRDLKP NIFLDS   VKIGD GLA +    V        ES +    K +   N    G VGT +Y+ PE+    +++Y  K D++SLGII+FEM ++P  TG+ER  +L  LR   IIFP++   + + +   +I+ LL HD  LRP+A ELL+S ++PPP+ +E EL + L     N +   Y+ M+ +LF++  S +  Y   + LY+  F+          F N +                L++ V   I  IF  HGAV    PL  P+      G      +D  G++V LP +LR+ FA F+  N ++  KRY+I  +++ + + R HPRE +E AFDI+T   +S    AEI+Y + +I ++    +     + +N+T+LF A++ H  +        S  + +L S+          K++A  K      + + L+ L K+ +   N + +   +++S  K+  +V +  K   +DL       P L V L  + + GLV+++     V  +F+  I+  RR      TV       IL++GG Y   +++ + P     G V    SAVGAS+ V  +  ++ S+E+       D+ V       M+ A+ V               V +LW+  I+A+I YD
Sbjct:   98 HQGKSLGVSERFGRNIYCGFEVNSGDFAVIYEWSLQWNKKMSKFFTTQERGRIENCKKQ-IHAAENEFHSLLRLEHPNLVQYLGLSVVEKEECIV----VYMLVEHVAGNSLNQSL-------TAHGP--------VPLDKLCHYTAQLLTALDYLHSNSVVHKQLGPSSVLVDSEGNIRVTDYSLSKRFADICKEDIFEQARVRFSEETAMPT--RTGKKGDVWNLGLMLLALSQGKEVKEYPVTVP------SSLPADFQDFLQKCLCLNDTERWTAQQLLDHSFLKPSSPKHLSQYQDNSPEDLAVDFASSVIPRSHILNAPFSSVLQHRQISRFVSEFEELQLLGKGAFGAVIKVQNKLDGCYYAVKRIQVNPASKQFRRIKGEVTLLSRLNHENIVRYYNAWIERHELPATGVLSNTDSSEPLSSADKAPQRKNA--------PERLNELGLPDDVEDIAPPPALSSSVEWSTSIERSSSARCSGRQSSDDDDDDDDDEEDVFGASFLPSNNDSRSDIIF---DNGDESMD---------EMSKVEPSKRPASDTTESTESD----RHLITAHYLYIQMEYCEK--STLRDTID-QGLHQDQNRLWRLFREILDGLAYIHEQGMIHRDLKPVNIFLDSLDHVKIGDFGLATDHPANVAAGKFEVEESGSAVMAKPDPTGN--MTGMVGTALYVSPEVQGNTKATYNQKVDLFSLGIILFEMSYRPMTTGAERIAVLSQLRGEPIIFPEDFTLREQGTQKRVIEWLLKHDPALRPTALELLKSELLPPPQMEESELHEVLQHTMANINGKSYRTMVAQLFAQNTSPVMDYAYDIDLYKGSFS----------FNNAK----------------LQQHVYESITRIFKKHGAVRVQTPLFLPRNRKLYDGIELACFMDHSGMLVTLPYDLRIAFARFVARNNVTHLKRYSIERVFRPKKLDRAHPRELLECAFDIITPVSNSLLPEAEIIYTISEIIQEFPVLQERNYNIYLNHTSLFKAVLLHSGVPEDKLSQASNILCDLMSEKL-------SKREAEAKFCNFSLSANCLQTLYKYIEQKGN-LQDLVPLLSSLTKQKTAVTQLAKQGLKDLEELTVLLPKLGVKLMVVINLGLVYKVQHHSGVIFQFVAFIRKRRR------TVP-----DILAAGGRYDHLILEFRGPV--STGPVP---SAVGASVAVDKVCAAIVSMEEPPTVSSYDVLVVPVGHSSMSKAIRV---------------VQKLWSTGIAADINYD 1260

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Cavefish
Match: EIF2AK4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:HGNC Symbol;Acc:HGNC:19687])

HSP 1 Score: 348.591 bits (893), Expect = 3.036e-98
Identity = 341/1228 (27.77%), Postives = 569/1228 (46.34%), Query Frame = 1

Query:  733 HRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQ--IRFSDLKRKQFEDLKSNWLSKL-------EEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKRFEELKGRG---HSNERTGASTIK-KGSKRYDLMKLGVLAKEL--------FFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFL---TCENLSKNSRQNSQ--TVDSAQDLTP-WHLFEK--SSRIPK-------EFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSS-------SETNNLQPNIDSSSIRNNTSSAISVIKPETTDSLLLDFQDKKNSIMFSANVNFSQIEHC-SRHFDLESFXXXXXXXXXXXXVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPFHGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGP-------VGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSL-LYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPKFINTESG--FFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDY-DSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRASLHDMVSKDVKNLDSDHFRSLTN--LTDKQ-KANIKSILLFNGSRLEDLEKHFKIFQNR--IHEGYDVINSAFKELKSVEEKL-KHFTEDLCFCVNFRPSL-VPLETIYH-GLVFQII--LKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLI 4194
            HR K I  + ++  + ++  N   G    +Y+  +R+S    K F   ++  + K        E +   L KL HP+L+ Y        E  +      +L+ +YV            C +     +   +  T + + ++ ++   +L A+ YLH  ++   ++   S+L+D    +++ ++ + KR  E+        ++ R    T+  +  K+ D+  LG+L   L        +   +P      + +P+DL DF+  C           ++L  H FL   + ++L      + +   VD A  + P  HL     S+ +PK       EFE +  LG+GAFG V+KV+N  D  YYA+KR+++N   K+  R   EV LLS LNHENIVRY+ +W E  +  S       S      P    +  R N    +  ++ +     L       +S+ +S ++  S    C +     +   D D D D ++ V      P       +I F   DN D   D+       DEL   +K   + +    DS+  P       I  YL IQME CE   +TL+  ID Q L+ +   +WR  REI+ G+ +IH + MIHRDLKP NIFLDS   VKIGD GLA   ++  T ++ +  + DN  +  +++ P       VGT +Y+ PE+    +++Y  K D++SLGII+FEM ++P  T SER  +L  LR+  I FP++       +  ++I  LLNHD   RP+A ELL+S+++PPP+ +E EL + L     N     Y+ M+ +LFS+  S +  Y   + +++  FN  IS+ K                        L+++V   I  +F  HGAV    PL  P+      G      +D  G++V LP +LR++FA F+  N +S  KRY+I  +++ + + R HPRE +E AFDIV     S    AE +Y + ++ ++    +    T+ +N+T+L  A++        LH  V +D  N  S+      +  LT ++ +A   ++ L N S    L+  +K  + +  + E   +INS  K+  +V  +L K   +DL         L V L+ + + GLV+++     V  +F+  IK     K  RT         I+++GG Y   +++ + P            SAVGASI +  +  ++ ++E+ +
Sbjct:  153 HRGKCIGESERLGRSVYHALNATSGEFAVVYEWILRWSKKIGKFFTSQETEKIEKCKKQIHGAESELNSLLKLQHPNLVHYQALSCTEREDCLV----VNLLAEYVPG----------CSL-----AHSLSTSTPVPVDELRHHATQLLSALDYLHSNSVVHKQLGASSVLLDSQGNVRLMDYSLAKRLAEVCREDVFEQAHVRFSEDTLPTRTGKKGDIWSLGLLILALSQGREVHEYPVTVP------SGLPADLQDFLKKCVCLNDAERWTTQQLLDHSFLRPPSPKSLPPCQEPSPEDLGVDFASTVIPQAHLLGGPFSTVVPKQFSRYFSEFEELQLLGKGAFGAVIKVQNKLDGCYYAVKRIQVNPGSKQFRRIKGEVTLLSRLNHENIVRYYNAWIERHETPSTGVLSSDSSEPPSTPERPPARRRVNALGLLDNVEDDAPPPAL------ASSVEWSTSLERSSSAKCKTADSSDDDDDDDDDDDDEEADVFCPSFLPSNSDSESDIIF---DNGDASKDS----VVQDEL---MKKKAVDTAESTDSEKPPL------IAHYLYIQMEYCEK--STLRDTID-QGLHRDCSRLWRLFREILDGIAYIHEQGMIHRDLKPVNIFLDSHDHVKIGDFGLAT--DHPATAASEKLEIDDNNSALVLKSDPTGNMTGMVGTALYVSPEVQGNTKATYNQKVDLFSLGIILFEMSYRPMATASERITVLSQLRKESIDFPEDFGDYESGAQKKVICWLLNHDPARRPTAVELLKSDLLPPPQMEESELHEVLQHTMANVMGKAYRTMVNQLFSQNPSHVMDYTYDIDMHKGSFN--ISSAK------------------------LQQYVYETITRVFRKHGAVRLQTPLFLPRNRKLYEGCELACFMDHSGMLVTLPYDLRVSFARFVGKNNVSHVKRYSIERVFRPRKLDRAHPRELLECAFDIVVPVTGSLLPEAETIYTISEVIQEFCALQERNYTIYLNHTSLLRAVL--------LHSGVPEDKLNQASNILCDAMSEKLTKREVEAKFCNLSLSNNS----LQTLYKFIEQKGELQELVPLINSLTKQKTAVVTQLAKQGLKDLEELTGLLKKLGVKLQVVVNLGLVYKVQHHCGVIFQFVAFIK-----KRKRTVP------DIVAAGGRYDHLIVQFRAP-----AATAPVPSAVGASIALDKICTAVAAMEEPV 1274

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Cavefish
Match: EIF2AK4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:HGNC Symbol;Acc:HGNC:19687])

HSP 1 Score: 325.479 bits (833), Expect = 4.304e-90
Identity = 290/1022 (28.38%), Postives = 474/1022 (46.38%), Query Frame = 1

Query:  733 HRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQ--IRFSDLKRKQFEDLKSNWLSKL-------EEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKRFEELKGRG---HSNERTGASTI-KKGSKRYDLMKLGVLAKEL--------FFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFL---TCENLSKNSRQNSQ--TVDSAQDLTPW-HLFEK--SSRIPK-------EFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQ-------KSSSETNNLQPNIDSSSIRNNTSSAISVIKPETTDSLLLDFQDKKNSIMFSANVNFSQIEHC-SRHFDLESFXXXXXXXXXXXXVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPFHGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGP-------VGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSL-LYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPKFINTESG--FFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDY-DSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSI 3606
            HR K I  + ++  + ++  N   G    +Y+  +R+S    K F   ++  + K        E +   L KL HP+L+ Y        E  +      +L+ +YV            C +     +   +  T + + ++ ++   +L A+ YLH  ++   ++   S+L+D    +++ ++ + KR  E+        ++ R    T+  +  K+ D+  LG+L   L        +   +P      + +P+DL DF+  C           ++L  H FL   + ++L      + +   VD A  + P  HL     S+ +PK       EFE +  LG+GAFG V+KV+N  D  YYA+KR+++N   K+  R   EV LLS LNHENIVRY+ +W E  +        S S      P    +  R N    +  ++ +     L       +S+ +S ++  S    C +     +   D D D D ++ V      P       +I F   DN D   D+       DEL   +K   + +    DS+  P       I  YL IQME CE   +TL+  ID Q L+ +   +WR  REI+ G+ +IH + MIHRDLKP NIFLDS   VKIGD GLA +     +E      + DN  +  +++ P       VGT +Y+ PE+    +++Y  K D++SLGII+FEM ++P  T SER  +L  LR+  I FP++       +  ++I  LLNHD   RP+A ELL+S+++PPP+ +E EL + L     N     Y+ M+ +LFS+  S +  Y   + +++  FN  IS+ K                        L+++V   I  +F  H  V    PL  P+      G      +D  G++V LP +LR++FA F+  N +S  KRY I  +++ + + R HPRE +E AFDIV     S    AE +Y + ++ ++    +    T+ +N+T+L  A++ H  +
Sbjct:  187 HRGKCIGESERLGRSVYHALNATSGEFAVVYEWILRWSKKIGKFFTSQETEKIEKCKKQIHGAESELNSLLKLQHPNLVHYQALSCTEREDCLV----VNLLAEYVPG----------CSL-----AHSLSTSTPVPVDELRHHATQLLSALDYLHSNSVVHKQLGASSVLLDSQGNVRLMDYSLAKRLAEVCREDVFEQAHVRFSEDTLPTRTGKKGDIWSLGLLILALSQGREVHEYPVTVP------SGLPADLQDFLKKCVCLNDAERWTTQQLLDHSFLRPPSPKSLPPCQEPSPEDLGVDFASTVIPQAHLLGGPFSTVVPKQFSRYFSEFEELQLLGKGAFGAVIKVQNKLDGCYYAVKRIQVNPGSKQFRRIKGEVTLLSRLNHENIVRYYNAWIERHETPSTGVLSSDSSEPPSTPERPPARRRVNALGLLDNVEDDAPPPAL------ASSVEWSTSLERSSSAKCKTADSSDDDDDDDDDDDDEEADVFCPSFLPSNSDSESDIIF---DNGDASKDS----VVQDEL---MKKKAVDTAESTDSEKPPL------IAHYLYIQMEYCEK--STLRDTID-QGLHRDCSRLWRLFREILDGIAYIHEQGMIHRDLKPVNIFLDSHDHVKIGDFGLATDHPATASEKLE---IDDNNSALVLKSDPTGNMTGMVGTALYVSPEVQGNTKATYNQKVDLFSLGIILFEMSYRPMATASERITVLSQLRKESIDFPEDFGDYESGAQKKVICWLLNHDPARRPTAVELLKSDLLPPPQMEESELHEVLQHTMANVMGKAYRTMVNQLFSQNPSHVMDYTYDIDMHKGSFN--ISSAK------------------------LQQYVYETITRVFRKH--VRLQTPLFLPRNRKLYEGCELACFMDHSGMLVTLPYDLRVSFARFVGKNNVSHVKRY-IERVFRPRKLDRAHPRELLECAFDIVVPVTGSLLPEAETIYTISEVIQEFCALQERNYTIYLNHTSLLRAVLLHSGV 1126

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Cavefish
Match: eif2ak1 (eukaryotic translation initiation factor 2 alpha kinase 1 [Source:NCBI gene;Acc:103026818])

HSP 1 Score: 170.629 bits (431), Expect = 6.533e-44
Identity = 151/493 (30.63%), Postives = 229/493 (46.45%), Query Frame = 1

Query: 1489 HPFLTCENLSKNSRQNSQTVDSAQDLTPWHLFE-KSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRL-NAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKS--SSETNNLQPNIDSSSIRNNTSSAISVIK---PETTDSLLLDFQDKKNSIMFSANVNFSQIEHCSRHFDLESFXXXXXXXXXXXXVELKDQSPRYKQPSVEISFGYSDNDDNP-LDNHHKKNTPDELSKQVKSMRLKSGYINDSQN-NPFHGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLI--------------WRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQK-RVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNI 2895
            H +L+ E  S  +R    T D         LF+ ++SR   EFE I  LG+G++G V KV+N  D Q+YA+K++ + N  +    + ++EV++LSSL H NIV Y  +W E +Q +  S     L+ + + SSI   +S      K    +T  S  +    + +  MF+ N+                                  ++P+   P V +    S  D  P L       + DE S     +   S    DSQ     H  + +    L IQM+LCE    +L+ +I ++      +L               +  +++I+ G+E+IHS  ++HRDLKP NIFL      VKIGD GLA    + V     Q +   +   N      VGT +Y  PE L  S Y +K+DMYS+G+I  E+ H PFGT  ER H LG LRR ++     +++ +     + IK L + D  LRP+ASELLQS++
Sbjct:   91 HKYLSSEGQSSATR----TKDG--------LFQAQTSRYLSEFEEISTLGKGSYGRVFKVKNKLDGQHYAVKKILIKNVSRDDCVKVLREVKVLSSLQHPNIVGYHTAWMEHVQPAVNSKWLGRLEESTNGSSIVFESSECSKDTKDPPTQTPQSTSVKSTPETSQAMFTKNM----------------------------------RNPQNFVPCVFLGKPRSVVDTCPALSWDTSVLSEDEASPNRVELNNNSYIDMDSQQWAEKHPKEVQFHLMLYIQMQLCE---RSLKDWIQERNCTPKAELAVAGGPYQPFDCEEAFNILKKILEGVEYIHSCGIMHRDLKPRNIFLHGHACHVKIGDFGLACK--DIVANKQEQLHFNSHIGKNSAHTTGVGTFVYASPEQLEGSHYDSKSDMYSVGVIAVELFH-PFGTEMERVHTLGELRRGKVPDTLAENWPL---LTKQIKLLTSADPSLRPNASELLQSDL 528

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Cavefish
Match: eif2ak1 (eukaryotic translation initiation factor 2 alpha kinase 1 [Source:NCBI gene;Acc:103026818])

HSP 1 Score: 166.392 bits (420), Expect = 3.068e-42
Identity = 149/501 (29.74%), Postives = 242/501 (48.30%), Query Frame = 1

Query: 1489 HPFLTCENLSKNSRQNSQTVDSAQDLTPWHLFE-KSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRL-NAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSS--IRNNTSSAISVIKPETTDSLLLDFQDKKNSIMFSANVNFSQIEHCSRHFDLESFXXXXXXXXXXXXVELKDQSPRYKQPSVEISFGYSDNDDNP-LDNHHKKNTPDELSKQVKSMRLKSGYINDSQN-NPFHGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLI--------------WRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQK-RVKIGDLGLA-----------MNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNI 2895
            H +L+ E  S  +R    T D         LF+ ++SR   EFE I  LG+G++G V KV+N  D Q+YA+K++ + N  +    + ++EV++LSSL H NIV Y  +W E          ++QP +++ +  +  N+S A+  +    TD  L +  +  + +  S+  +    +  ++     S   +   S +  +  +++  P+   P V +    S  D  P L       + DE S     +   S    DSQ     H  + +    L IQM+LCE    +L+ +I ++      +L               +  +++I+ G+E+IHS  ++HRDLKP NIFL      VKIGD GLA           ++FN+ + ++A   +              VGT +Y  PE L  S Y +K+DMYS+G+I  E+ H PFGT  ER H LG LRR ++     +++ +     + IK L + D  LRP+ASELLQS++
Sbjct:  120 HKYLSSEGQSSATR----TKDG--------LFQAQTSRYLSEFEEISTLGKGSYGRVFKVKNKLDGQHYAVKKILIKNVSRDDCVKVLREVKVLSSLQHPNIVGYHTAWME----------HVQPAVNNPTKPMLPNSSPALGSLS--QTDGRLEESTNGSSIVFESSECSKDTKDPPTQTPQSTSVKSTPETSQAMFTKNMRN--PQNFVPCVFLGKPRSVVDTCPALSWDTSVLSEDEASPNRVELNNNSYIDMDSQQWAEKHPKEVQFHLMLYIQMQLCE---RSLKDWIQERNCTPKAELAVAGGPYQPFDCEEAFNILKKILEGVEYIHSCGIMHRDLKPRNIFLHGHACHVKIGDFGLACKDIVANKQEQLHFNSHIGKNAESAHTTG-----------VGTFVYASPEQLEGSHYDSKSDMYSVGVIAVELFH-PFGTEMERVHTLGELRRGKVPDTLAENWPL---LTKQIKLLTSADPSLRPNASELLQSDL 576

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Cavefish
Match: eif2ak1 (eukaryotic translation initiation factor 2 alpha kinase 1 [Source:NCBI gene;Acc:103026818])

HSP 1 Score: 166.007 bits (419), Expect = 6.778e-42
Identity = 142/471 (30.15%), Postives = 232/471 (49.26%), Query Frame = 1

Query: 1579 LFE-KSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRL-NAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSS--IRNNTSSAISVIKPETTDSLLLDFQDKKNSIMFSANVNFSQIEHCSRHFDLESFXXXXXXXXXXXXVELKDQSPRYKQPSVEISFGYSDNDDNP-LDNHHKKNTPDELSKQVKSMRLKSGYINDSQN-NPFHGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLI--------------WRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQK-RVKIGDLGLA-----------MNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNI 2895
            LF+ ++SR   EFE I  LG+G++G V KV+N  D Q+YA+K++ + N  +    + ++EV++LSSL H NIV Y  +W E          ++QP +++ +  +  N+S A+  +    TD  L +  +  + +  S+  +    +  ++     S   +   S +  +  +++  P+   P V +    S  D  P L       + DE S     +   S    DSQ     H  + +    L IQM+LCE    +L+ +I ++      +L               +  +++I+ G+E+IHS  ++HRDLKP NIFL      VKIGD GLA           ++FN+ + ++A   +              VGT +Y  PE L  S Y +K+DMYS+G+I  E+ H PFGT  ER H LG LRR ++     +++ +     + IK L + D  LRP+ASELLQS++
Sbjct:  176 LFQAQTSRYLSEFEEISTLGKGSYGRVFKVKNKLDGQHYAVKKILIKNVSRDDCVKVLREVKVLSSLQHPNIVGYHTAWME----------HVQPAVNNPTKPMLPNSSPALGSLS--QTDGRLEESTNGSSIVFESSECSKDTKDPPTQTPQSTSVKSTPETSQAMFTKNMRN--PQNFVPCVFLGKPRSVVDTCPALSWDTSVLSEDEASPNRVELNNNSYIDMDSQQWAEKHPKEVQFHLMLYIQMQLCE---RSLKDWIQERNCTPKAELAVAGGPYQPFDCEEAFNILKKILEGVEYIHSCGIMHRDLKPRNIFLHGHACHVKIGDFGLACKDIVANKQEQLHFNSHIGKNAESAHTTG-----------VGTFVYASPEQLEGSHYDSKSDMYSVGVIAVELFH-PFGTEMERVHTLGELRRGKVPDTLAENWPL---LTKQIKLLTSADPSLRPNASELLQSDL 614

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007738.1 (pep scaffold:Pmarinus_7.0:GL476507:25231:69652:1 gene:ENSPMAG00000006975.1 transcript:ENSPMAT00000007738.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 353.599 bits (906), Expect = 1.809e-99
Identity = 348/1269 (27.42%), Postives = 547/1269 (43.10%), Query Frame = 1

Query:  664 LEFEHCSDRSSGCLVYKGICDGQHRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQ------IRFSDLK----RKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQL-KICNFCIGKRFEELKGRGHSNERTGASTI--------------------KKGSKRYDLMKLGVLAKELFFFGIPETEREET---DMPSDLIDFITSC-------NSNIEELKLHPFL----TCENLSKNSRQNSQT--VDSAQDLTPW--------HLFEKS--SRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTS-----SAISVI--------KPETTDSLLLDFQDKKNSIMFSANVNFSQIEHCSRHFDLESFXXXXXXXXXXXXVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPFHGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTES-------ANQEYVKDNGLSNDVENGPVGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSL-LYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSH-GAVEFLAPLLSPK----FINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDYDSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRASLHDMVSK---DVKNLDSDHFRSLTNLTDKQKANIKSILLFN-----GSRLEDLEKHFKIFQNRIHEGYDVINSAFKELKSVEEKLKHFTEDLCF--CVNFRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIE 4185
            L+F    +RS    VY+G C G+        +  ++   FN  +   G  V +Y+      +   DL+    ++  E  +   +     +   L KL H  L+ Y G   Q  E          L +  +  +  G  L           +G  A    L +  +  Y   +L  + YLH  ++    + + S+L+D    L ++ ++ + +R  +         R G   +                     K  K+ D+ +LGVL ++L      E+  ++T   D+P DL +F++ C         + ++L  HPF+    +C   ++  R ++    V+ A+ L P         HL  +   SR   EFE +  LG+GAFG V+KV+N  D  YYA+KR+ +N   ++  R   EV LLS LNHENIVRY+ +W E  + S S+     P++ +   R         S I+ +         P T  +  +       S+ +S +   S    C       S SDS  + D D  V  +   P       ++ F  S   +   ++H    +  ELSK                  P       +  +L IQME CE   +TL+  ID + L+M    +WR  REI+ GL +IH + MIHRDLKP NIFLDS   VKIGD GLA +     TES       AN E   +N        G +GT +Y+ PE+L   ++ Y  K D+YSLGII FEM + P  T SER  +L  LR P I FP++ H +       +I+ LLNHD   RP+A ELL+S  +PPP+ +E EL + L     N     Y+ ++ ELFS+  +    +   + +++  F    S T S+V                      + +V   +  +F  H GAV+   PLL PK    + N E+  F  +D  G +V LP++LR+ FA F+  N ++  KRY +  +++ + + R HPRE IE AFDIVT        AEI+Y   +I ++    +     + +N+T L  A++ H  I    H  V     D  N      + + +L           LLF+     G+R+ D+    +    +  +   ++      L  +EE +KH  E +C    V     LV    ++ GL+FQ    V+ K   V+                    IL++GG Y + +   + P     G V    SAVG S+ +  +  ++ S E
Sbjct:  277 LKFHTQGERS----VYRGKCIGE--------STHLRRQVFNALDNSTGELVVVYEWVLRWPVGHPDLRTSEDKEHMEKCRKQ-VHGASAELQSLLKLRHEALVPYLGMESQETETA--------LTVHLLAEHVSGTSL-----------AGHLARTGPLPVETVRQYASQLLHVLDYLHASSVVHRDLRLSSVLLDCATGLLRVADYSLARRLADACVASEQPGRPGVPRVHFVEPECGPGAGAAAARSQPPKTGKKGDVWRLGVLLEQLAVGR--ESSNDDTPPADLPIDLREFLSRCLCMDDKERWSTQQLLEHPFIKTPVSCSPSTRPPRVHAAADDVNYAEALIPSRDILGMCSHLDSQRQLSRYYTEFEELALLGKGAFGAVIKVKNKLDGCYYALKRIAVNPTSRQFRRIKGEVTLLSRLNHENIVRYYNAWIERYELSPSDAKE-TPDVANVRGRGPAMHLSRLSGIAGLDDVEANAPPPVTASTRTV----ASGSVEWSMSYEHSVSARCGPSTTGGSPSDSSDEDDEDDVVFGRSFVPSGSDSDCDVVFENSGEKNQSEESHDSSLSDKELSKN---------------RAPVCQPTPTVVHHLFIQMEYCE--RSTLRDTID-KGLHMETDRMWRLFREILDGLAYIHEQGMIHRDLKPVNIFLDSSDSVKIGDFGLATDHRLVATESSKIDVSTANIELPTENMDVTGGLTGQIGTALYVSPEVLGNIKAVYNQKVDLYSLGIIFFEMTYHPMTTASERVAVLQKLRLPTIQFPEDFHPQTLKQQAFIIRWLLNHDAGQRPTAQELLKSEQLPPPQLEESELHEVLRHTLANPDGKAYRSLVGELFSQRVALPADFTYDIDVHKGNF----SATSSMV----------------------QSYVYETVCKVFERHGGAVKLHMPLLMPKNRYLYENNEAAVF--MDHSGFLVLLPHDLRVPFARFVARNNVTNLKRYCVERVFRPRKLHRAHPRELIECAFDIVTSAGGCLPDAEIIYTTSEIIQEFPVLQDRNYCVHLNHTALIKAILIHCGIPEDKHHQVYGILCDTMNEKRTKQQIMASLCSLGLHPDTVTLLFHFVERRGNRM-DVLAALRPLAGKHSKTVGLVKQG---LSYLEEVMKHL-EYMCIRHQVVISLGLVYKVQLHSGLIFQFTSFVRRKNKTVL-------------------DILAAGGRYDNLISMFRAP--QSSGPV---PSAVGVSLALDKITSAILSKE 1431

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Sea Lamprey
Match: eif2ak1 (eukaryotic translation initiation factor 2-alpha kinase 1 [Source:ZFIN;Acc:ZDB-GENE-061013-268])

HSP 1 Score: 109.383 bits (272), Expect = 1.035e-24
Identity = 67/213 (31.46%), Postives = 111/213 (52.11%), Query Frame = 1

Query: 2293 LMIQMELCEGQYNTLQKYIDDQKLYMNE----------KLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDS-QKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNII 2898
            L IQM+LCE    +L++++ D+   + +          K   R  ++++ G+ +IHS+ ++HRDLKP NIFL      V+IGD GLA      +     +      G+        VGT +Y  PE L+ S Y  K+DMYS+GI++FE+  +PFGT  ER   +  LR   +  P    + +++ ++ L   L   D   RP+  ++L +++I
Sbjct:  304 LYIQMQLCE---RSLKEWLMDRNALLQQCTGRYALVDGKQTTRMFQQLLEGVHYIHSRGIMHRDLKPRNIFLHGPDHHVRIGDFGLACQ---DIVYEDPRLIRAGAGVVGSAHTSGVGTTLYAAPEQLSGSHYDFKSDMYSVGIVLFEL-FQPFGTEMERVKTIQELRGGVVSSPFTAQWPVQAKYIGL---LTQDDPAARPNPQDILTTDLI 506

HSP 2 Score: 70.8626 bits (172), Expect = 1.671e-12
Identity = 33/79 (41.77%), Postives = 49/79 (62.03%), Query Frame = 1

Query: 1591 SSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKK-AARTMKEVQLLSSLNHENIVRYFASWSEEMQ 1824
            +SR   EFE +C+LG G +G V KVRN  D Q YA+K+V +    +    + ++EV+LL+ L H +IV Y  +W E +Q
Sbjct:   98 TSRYLSEFEEVCRLGRGGYGQVFKVRNKLDRQLYAVKKVLIKGSGESICKKVLREVKLLAGLQHLSIVGYHGAWLELLQ 176

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006924.1 (pep scaffold:Pmarinus_7.0:GL476537:325917:352372:-1 gene:ENSPMAG00000006216.1 transcript:ENSPMAT00000006924.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 102.834 bits (255), Expect = 3.817e-22
Identity = 73/215 (33.95%), Postives = 113/215 (52.56%), Query Frame = 1

Query: 2290 YLMIQMELCEGQYNTLQKYIDDQKLYMNEKL--IWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFE-MCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFV---ELIKSLLNHDQLLRPSASELLQSNI---IPPP 2907
            +L IQM+LC  + ++L+++++ +    +          ++IV+ ++ +H   ++HRDLKP NIF      VKIGD GL        TE   +  V      +    G VGT +Y+ PE L   +Y++K D++SLG+I+FE +C  PFGT  ER   L   R    IFPD       +S V   +L++ LL  D  LRP A E+ Q+ +   + PP
Sbjct:  773 FLYIQMQLC--RKDSLKEWLNSRTNAADRSRAECLTIFQQIVNAVQFLHVSGLMHRDLKPSNIFFSLDGVVKIGDFGLVTG-----TEHEEEGAVLTPMPGHIHHTGQVGTKLYMSPEQLAGRAYSHKVDIFSLGLILFELLC--PFGTQMERIKTLSAAR--NRIFPD----PFVTSNVDEHQLVERLLAQDPSLRPEAEEIAQAALFAELDPP 972

HSP 2 Score: 68.5514 bits (166), Expect = 1.135e-11
Identity = 43/124 (34.68%), Postives = 63/124 (50.81%), Query Frame = 1

Query: 1504 CENLSKNSRQNSQTVDSAQDLTPWHLFEKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSS 1875
            C+ L+K     +Q  D        +L    SR   +FE +  LG G FG V + RN  D+ +YAIKR+RL    +   + M+EV+ L+ L H+ +VRYF +W E   +   E  + Q   D SS
Sbjct:  456 CDILTKQPNHPAQAED--------NLTTYVSRYLTDFEHVQCLGRGGFGVVFEARNKVDDCHYAIKRIRLPNRIQAREKVMREVKALAKLEHQGVVRYFNAWLEMPPQDWQEEKDKQWISDCSS 571

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Sea Lamprey
Match: nek2 (NIMA-related kinase 2 [Source:ZFIN;Acc:ZDB-GENE-040426-752])

HSP 1 Score: 90.8929 bits (224), Expect = 4.556e-19
Identity = 62/214 (28.97%), Postives = 102/214 (47.66%), Query Frame = 1

Query: 2293 LMIQMELCE-GQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIH-----SKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMCH--KPFGTGSERYHI----LGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNII 2898
            L I ME CE G   +L       + ++ E+ + R + ++V  L   H      + ++HRDLKP N+FLD++  VK+GD GLA    +            D   +       VGT  Y+ PE  ++ SY  K+D++SLG +++E+C    PF   +++        G  RR    + DE +        E+I  +LN    LRPS  +++Q  ++
Sbjct:   51 LYIVMEYCECGDLASLISRCRRDRRFLEEEFVLRVLAQLVLALRECHRRTAGGRTVLHRDLKPANVFLDARHNVKLGDFGLARILQH------------DTSFAKTF----VGTPYYMSPEQFSRMSYNEKSDIWSLGCLLYELCALVPPFTAMNQKQLAEKIKEGKFRRIPYRYSDELN--------EIISKMLNLKDYLRPSIEQIMQYGLL 240

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007414.1 (pep scaffold:Pmarinus_7.0:GL476981:230882:244405:1 gene:ENSPMAG00000006689.1 transcript:ENSPMAT00000007414.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 85.5001 bits (210), Expect = 7.072e-18
Identity = 58/205 (28.29%), Postives = 98/205 (47.80%), Query Frame = 1

Query: 2293 LMIQMELCEGQYNTLQKYIDDQK-LYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMC--HKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNII 2898
            L I M+ CEG    L K I+ QK +  +E  I  ++ ++   L+H+H + ++HRD+K  NIFL     V++GD G+A   N+                + ++    +GT  Y+ PE+     Y +K+DM++LG +++EMC     F  G+ +  +L  +R      P   H+   S     +  LL  D   RP  + LL   ++
Sbjct:   76 LYIVMDYCEG--GDLFKRINAQKGVLFSEDQILDWLVQLCLALKHVHDRKILHRDIKSQNIFLTKAGIVQLGDFGIARVLNS----------------TTELARTCIGTPYYLSPEICENKPYNHKSDMWALGCVLYEMCTLRHAFEAGNMKNLVLKIIRGS--FPPVSAHY--SSELRSTLSGLLRRDPRARPPVAVLLSRPLL 258

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Yeast
Match: GCN2 (Protein kinase; phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control [Source:SGD;Acc:S000002691])

HSP 1 Score: 174.096 bits (440), Expect = 5.479e-44
Identity = 193/710 (27.18%), Postives = 312/710 (43.94%), Query Frame = 1

Query: 2293 LMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVT----ESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSS-YTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFP---DEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLA-PLLSPKF-INTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRD--HPRERIEAAFDIVTDYDSPNK--TAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRASLHDMVSKDVKNLDSDHFRSLTNLTDKQKA--NIKSILLFNGSRLEDLEKHFKIFQNRIHEGYDVINSAFKELKSVEEKLKHFTEDLCFCVNFRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAV-------NVVVQ--YDIEMDGIIWDLVSRLWAANISAEI 4347
            L IQME CE +  TL   I  + L       WR  R+I+  L +IHS+ +IHRDLKP NIF+D  + VKIGD GLA N +  +     +S N     DN  S       +GT MY+  E+L+ +  Y  K DMYSLGII FEM + PF TG ER +IL  LR   I FP   D+   K++   + L   L++HD   RP A  LL S  +P     E+                     +KE    L +    ++   + +S FN   S T  ++F N   P S+       F   L+  +T  +  IF  HG +E  A P + PK  I      + +LD  G V+ L  +L    A +L  N     K+Y +  +Y+     R    PR+  E  FDI++   S +    AE L ++ +I       E       +N+ ++  ++ N  +I  +   +VS+ +  +     RS   + ++ KA  NI S  L N   L D    F+  + R+++   +I+S    LK +E+ L H ++ L +       L PLE +   +V   +      F       +             +  ++++GG Y + +    RP   +  +  +   AVG ++      ++++ I Q          + +   +L ++AV       +V++    +  +D I   +++ LW  NI A++
Sbjct:  784 LFIQMEYCENR--TLYDLIHSENLNQQRDEYWRLFRQILEALSYIHSQGIIHRDLKPMNIFIDESRNVKIGDFGLAKNVHRSLDILKLDSQNLPGSSDNLTS------AIGTAMYVATEVLDGTGHYNEKIDMYSLGIIFFEMIY-PFSTGMERVNILKKLRSVSIEFPPDFDDNKMKVEKKIIRL---LIDHDPNKRPGARTLLNSGWLPVKHQDEV---------------------IKEALKSLSNPSSPWQ-QQVRESLFNQSYSLTNDILFDN-SVPTSTP------FANILRSQMTEEVVKIFRKHGGIENNAPPRIFPKAPIYGTQNVYEVLDKGGTVLQLQYDLTYPMARYLSKNPSLISKQYRMQHVYRPPDHSRSSLEPRKFGEIDFDIISKSSSESGFYDAESLKIIDEILTVFPVFEKTNTFFILNHADILESVFNFTNIDKAQRPLVSRMLSQV--GFARSFKEVKNELKAQLNISSTAL-NDLELFDFRLDFEAAKKRLYKL--MIDSP--HLKKIEDSLSHISKVLSY-------LKPLE-VARNVVISPLSNYNSAFY------KGGIMFHAVYDDGSSRNMIAAGGRYDTLISFFARPSGKKSSNTRK---AVGFNL----AWETIFGIAQNYFKLASGNRIKKRNRFLKDTAVDWKPSRCDVLISSFSNSLLDTIGVTILNTLWKQNIKADM 1424

HSP 2 Score: 82.8037 bits (203), Expect = 4.360e-16
Identity = 39/75 (52.00%), Postives = 54/75 (72.00%), Query Frame = 1

Query: 1594 SRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEE 1818
            SR   +FE I  LG+GAFG VVK RN  D++YYAIK++R    ++K +  + EV LL+SLNH+ +VRY+A+W EE
Sbjct:  593 SRYASDFEEIAVLGQGAFGQVVKARNALDSRYYAIKKIR--HTEEKLSTILSEVMLLASLNHQYVVRYYAAWLEE 665

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Yeast
Match: SSK22 (MAP kinase kinase kinase of HOG1 mitogen-activated signaling pathway; functionally redundant with Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p; SSK22 has a paralog, SSK2, that arose from the whole genome duplication [Source:SGD;Acc:S000000669])

HSP 1 Score: 83.9593 bits (206), Expect = 1.621e-16
Identity = 58/203 (28.57%), Postives = 100/203 (49.26%), Query Frame = 1

Query: 2299 IQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSSYTNKA---DMYSLGIIVFEMC--HKPFGTGSERYHILGNL---RRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELL 2883
            I ME CEG   +L   +D  ++  +E +   +  E++ GL ++H   ++HRD+KP NI LD    +K  D G A       T +     V+D G+     N  +GT MY+ PE ++ S+   K    D+++LG +V EM    +P+      + I+ ++   R P++   DE    + ++    ++  L  D  +R +A ELL
Sbjct: 1110 IFMEYCEG--GSLASLLDHGRIE-DEMVTQVYTFELLEGLAYLHQSGVVHRDIKPENILLDFNGIIKYVDFGTARTVVGSRTRTVRNAAVQDFGVETKSLNEMMGTPMYMAPETISGSAVKGKLGADDVWALGCVVLEMATGRRPWSNLDNEWAIMYHVAAGRIPQLPNRDE----MTAAGRAFLERCLVQDPTMRATAVELL 1305

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Yeast
Match: CLA4 (Cdc42p-activated signal transducing kinase; member of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p; CLA4 has a paralog, SKM1, that arose from the whole genome duplication [Source:SGD;Acc:S000005242])

HSP 1 Score: 80.1073 bits (196), Expect = 1.935e-15
Identity = 47/144 (32.64%), Postives = 70/144 (48.61%), Query Frame = 1

Query: 2293 LMIQMELCEGQYNTLQKYI------DDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEM 2706
            L + ME  EG   +L   I      D+    + E  I   +RE   GL+ +H K++IHRD+K  N+ LD++ RVKI D G      +K ++ A                  VGT  ++ PE++ Q  Y  K D++SLGI+  EM
Sbjct:  636 LWVVMEFMEG--GSLTDIIENSPTNDNSHSPLTEPQIAYIVRETCQGLKFLHDKHIIHRDIKSDNVLLDTRARVKITDFGFCARLTDKRSKRATM----------------VGTPYWMAPEVVKQREYDEKIDVWSLGIMTIEM 761

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Yeast
Match: KIN82 (Putative serine/threonine protein kinase; implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases); involved in the phosphorylation of upstream inhibitory kinase Ypk1p along with Fpk1p; has a redundant role in the cellular response to mating pheromone; KIN82 has a paralog, FPK1, that arose from the whole genome duplication [Source:SGD;Acc:S000000687])

HSP 1 Score: 79.337 bits (194), Expect = 3.538e-15
Identity = 64/214 (29.91%), Postives = 101/214 (47.20%), Query Frame = 1

Query: 2260 FHGNKTKIEKYLMIQMELCEGQ--YNTLQ----KYI--DDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDV--ENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEM---CHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLR 2862
            +H  +TK   YL + ME C G   +  LQ    K I  +D K Y +E         +V+ LE++H    I+RDLKP NI L     V + D  L++        +       D  + +D    N  VGT  Y+ PE++  + +T   D ++LGI+++EM   C  PF  G        N+   ++ FP +K  ++  +  +LIK LLN ++  R
Sbjct:  388 YHSFQTK--DYLYLCMEYCMGGEFFRALQTRKSKCIAEEDAKFYASE---------VVAALEYLHLLGFIYRDLKPENILLHQSGHVMLSDFDLSIQATGSKKPTMKDSTYLDTKICSDGFRTNSFVGTEEYLAPEVIRGNGHTAAVDWWTLGILIYEMLFGC-TPF-KGDNSNETFSNILTKDVKFPHDK--EVSKNCKDLIKKLLNKNEAKR 586

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Yeast
Match: STE11 (Signal transducing MEK kinase; involved in pheromone response and pseudohyphal/invasive growth pathways where it phosphorylates Ste7p, and the high osmolarity response pathway, via phosphorylation of Pbs2p; regulated by Ste20p and Ste50p; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000004354])

HSP 1 Score: 78.9518 bits (193), Expect = 4.439e-15
Identity = 59/175 (33.71%), Postives = 89/175 (50.86%), Query Frame = 1

Query: 2374 EKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEM--CHKPFGTGSERYHI--LGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQ 2886
            E LI  F R+I+ G+ ++H KN+IHRD+K  NI +D +  VKI D G++     K     N++  K   L         G++ ++ PE++ Q++ T KAD++S G +V EM     PF   S+   I  +G    PEI  P     + K+     ++     D   RPSA ELLQ
Sbjct:  553 ESLITNFTRQILIGVAYLHKKNIIHRDIKGANILIDIKGCVKITDFGIS-----KKLSPLNKKQNKRASLQ--------GSVFWMSPEVVKQTATTAKADIWSTGCVVIEMFTGKHPFPDFSQMQAIFKIGTNTTPEI--PSWATSEGKN----FLRKAFELDYQYRPSALELLQ 708

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Nematostella
Match: EDO48785 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RIC0])

HSP 1 Score: 105.531 bits (262), Expect = 9.270e-23
Identity = 68/207 (32.85%), Postives = 113/207 (54.59%), Query Frame = 1

Query: 2290 YLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVEN---GPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDE--KHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNI 2895
            YL IQM+LC  Q  +L+ ++ +      +   +    +IV+ +E+ H++ M+HRDLKP NIF      VK+GD GL        T+  N   +++     D++    G VGT +Y+ PE +   +Y+ K D++SLG+I+FE+ H PF T  ER  ++ N+++   I P E      ++S  V  + S L  +   RP+A+E+  S+I
Sbjct:  778 YLYIQMQLC--QRESLKDWLKNNST-REDIFCFNVFEQIVNAVEYFHNRGMMHRDLKPSNIFFSLDGLVKVGDFGLVT-----ATKQDNIHEIQETDNPGDLDGNHTGQVGTQLYMSPEQIEGKAYSFKVDIFSLGLILFELFH-PFSTEMERIKVMSNVKK--RIMPAEFKTSMTLQSQLVTWMTSDLPTE---RPTAAEIQSSDI 970

HSP 2 Score: 70.0922 bits (170), Expect = 7.530e-12
Identity = 36/75 (48.00%), Postives = 46/75 (61.33%), Query Frame = 1

Query: 1591 SSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSE 1815
            SSR   +FE    LG+G FG V + RN  D+  YAIKRVRL    +  A+ M+EV+ L+ L H  IVRYF +W E
Sbjct:  483 SSRYLTDFEHELCLGKGGFGLVFQARNKVDDCQYAIKRVRLPNCDEARAKVMREVKALAKLEHSGIVRYFNAWFE 557

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Nematostella
Match: EDO37413 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFU9])

HSP 1 Score: 99.3673 bits (246), Expect = 1.239e-22
Identity = 68/207 (32.85%), Postives = 106/207 (51.21%), Query Frame = 1

Query: 2293 LMIQMELCE-GQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSK----NMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGP--VGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMC--HKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQ 2886
            + I ME CE G    L K    +K Y  E L+W+ + ++V  ++  H +    +++HRDLKP N+FLD+   VK+GD GLA                    LS+D       VGT  Y+ PE +N+ SY  K+D++SLG +++E+C    PF T  ++  +   ++      P   H+   SS  +LI S+L  +  +RPS  ELL+
Sbjct:   83 IYIVMEFCENGDLGALIKKHKREKRYTAEDLVWKLLYQLVLAVQECHRRKDGGHVLHRDLKPANVFLDANMNVKLGDFGLA------------------RVLSHDTSFAKTFVGTPYYMSPEQVNKRSYNEKSDIWSLGCLMYELCALSPPF-TAMDQIRLEAKIKVGR-FHPIPSHY--SSSLSQLINSMLQVNDEMRPSIDELLR 267

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Nematostella
Match: EDO46416 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQ17])

HSP 1 Score: 101.293 bits (251), Expect = 1.214e-21
Identity = 75/202 (37.13%), Postives = 100/202 (49.50%), Query Frame = 1

Query: 2239 NDSQNNPFH--GNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLY---MNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKR----------VKIGDLGLAMN----FNNKVTESAN--QEYVKDN----GLSN-DVENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRR 2766
            ND   +P      K  +   L IQM+LC     TL  ++  +      ++        R+IV G+ +IHS+ ++HRDLKP NIFL S  R          VKIGD GLA      + +  +  A+  +     N    GLS  D     VGT  Y  PE L  + Y NKADMYSLGII+FE+ + PFGT  ER   + NLR+
Sbjct:  385 NDKSESPSTQVARKKTLGMMLFIQMQLCT---TTLCDWLQSRNFKGGNVDTVASMAIFRQIVEGVRYIHSQGLMHRDLKPRNIFLQSLTRRDEQTTAPLQVKIGDFGLARKDAVLYPDTSSPLASLIEPLSHHNPFLPGLSTKDAPTCGVGTCTYAAPEQLRNTDYANKADMYSLGIILFEL-YWPFGTEMERVTSIKNLRQ 582

HSP 2 Score: 61.6178 bits (148), Expect = 2.649e-9
Identity = 30/76 (39.47%), Postives = 48/76 (63.16%), Query Frame = 1

Query: 1591 SSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKK-AARTMKEVQLLSSLNHENIVRYFASWSE 1815
            +SR   EF  + +LG+G FG V+KV+N  D + YA+K+V L         + ++EV++L+ LNH N+V Y ++W E
Sbjct:  170 TSRYKDEFVEMGRLGKGGFGSVLKVKNKLDGREYAVKKVLLKETDPGLCLKILREVKVLARLNHVNVVGYHSAWLE 245

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Nematostella
Match: EDO49690 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG02])

HSP 1 Score: 95.5153 bits (236), Expect = 2.681e-21
Identity = 68/205 (33.17%), Postives = 104/205 (50.73%), Query Frame = 1

Query: 2290 YLMIQMELCEGQYNTLQKYIDDQK-LYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMC--HKPFGT---GSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQ 2886
            +L I M  CEG    L   +  QK + ++E  I  +  +I   L+++H K+++HRDLK  NIFL   K +K+GDLG+A     +V ES           S+D+    +GT  Y+ PE+ +   Y +K+D+++LG  ++EMC     F      S  Y IL     P    P +      +    +IKS+L+ D   RPSAS LL+
Sbjct:   78 FLYIAMNFCEG--GDLYSRLKAQKGIPLDENQIVEWFVQIAMALQYMHEKHILHRDLKTQNIFLTKSKIIKVGDLGIA-----RVLES-----------SSDMATTLIGTPYYMSPELFSNKPYNHKSDVWALGCCLYEMCTLRHAFNAKDMSSLVYKILKGKTPP---LPKQ----YSTDLCSIIKSMLDQDPDKRPSASRLLR 257

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Nematostella
Match: EDO34646 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNS0])

HSP 1 Score: 92.8189 bits (229), Expect = 2.665e-20
Identity = 48/146 (32.88%), Postives = 84/146 (57.53%), Query Frame = 1

Query: 2293 LMIQMELCE-GQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMC--HKPF 2721
            L I +EL + G  + + K+   QK  + E+ +W++  ++ + LEH+HS+ ++HRD+KP N+F+ +   VK+GDLGL   F++K T +                +  VGT  Y+ PE + ++ Y  K+D++SLG +++EM     PF
Sbjct:   94 LNIVLELADAGDLSRMIKHFKKQKRLIPERTVWKYFVQLTAALEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAA----------------HSLVGTPYYMSPERIKENGYNFKSDIWSLGCLLYEMAALQSPF 223

HSP 2 Score: 52.7582 bits (125), Expect = 5.638e-7
Identity = 27/70 (38.57%), Postives = 43/70 (61.43%), Query Frame = 1

Query: 1612 FELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAAR--TMKEVQLLSSLNHENIVRYFASWSE 1815
            FE+  K+G G F  V K R   +NQ  A+K++++  +    AR   +KE+ LL  LNH N+++Y+AS+ E
Sbjct:   21 FEIEKKIGRGQFSVVYKARCTANNQIVALKKIQIFDMMDAKARQDCIKEIDLLKQLNHPNVIKYYASFIE 90

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Medaka
Match: EIF2AK4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:NCBI gene;Acc:101172779])

HSP 1 Score: 361.303 bits (926), Expect = 1.148e-101
Identity = 304/1035 (29.37%), Postives = 494/1035 (47.73%), Query Frame = 1

Query:  703 LVYKGICDGQHRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQIR----------FSDLKRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDN---ALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKRF-----EELKGRGHSNERTGASTIKKGSKRYDLMKLGVL------AKELFFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFLTCENLSKN--SRQNSQTVDSAQDL------------TPWH--LFEKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSS-AISVIKPETTDSLLL--DFQDKK------NSIMFSANVNFSQIEHCSRHFDLESFXXXXXXXXXXXXVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPFHGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGP-------VGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFT--LKRFVTRYIESIFVSHGAVEFLAPLLSPK----FINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDY-DSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALM 3591
            +V+KG C G+        + ++  + +  F    G    +Y+            F+  ++ + E+ K   +   E +F  L +L+H +L+ Y        E  +  T H  L++++V                  PG+  N      + + + K+ +Y   +L A+ YLH  ++    +   S+L+D +  +++ ++ + KRF     E++  + H     G     K  K+ D+  LG++       KE+  + +         +P+D  DF+  C           ++L  H FL    L KN    Q +   D A+D             +P+   L  + SR   EFE +  LG+GAFG V+KV+N  D  YYA+KR+++N   K+  R   EV LLS LNHENIVRY+ +W E  +  S+    +  N DSS  ++       S   P+  + L L  + +D        +S+ +S ++  S    CS H   +   D D +    +S    D   R      +I F   DN D          + DE+S+  KS R  +    D+  +     +  I  YL IQME CE   +TL+  I DQ L++++  +WR  REI+ GL +IH + MIHRDLKP NIFLDSQ  VKIGD GLA +    V  +A +  ++D+G +  ++  P       VGT +Y+ PE+    +++Y  K D++SLGII+FEM ++P  TG+ER  +LG LR   I  P++     + +  ++I+ LL HD  LRP+A ELL+S ++PPP+ +E EL + L     N +   Y+ M+ +LF++  S +  Y   +                            + +G+F   T  L++ V   I  IF  HGAV    PLL P+    +  +E   F  +D  G++V LP +LRM FA F+  N I+  KRY+I  +++ + + R HPRE +E AFDIVT   +S    AEI+Y + +I ++    +     + +N+T+L  A++
Sbjct:  156 VVHKGKCLGE--------SQRLGRSVYCAFEANSGEFSVLYEWSLRWSKKMGKFFTSQEKGKIENCKKQ-IHAAENEFNSLLRLSHQNLVHYMALSSIEKEDCL--TVH--LLVEHV------------------PGTSLNQSLGADSPVLLDKLCHYAAQLLSALDYLHTNSVVHKHLGASSVLVDSEGTVRLTDYSLSKRFSDICKEDIFEQAHVRFSEGVVMPTKTGKKGDVWNLGLMLLALSQGKEVMEYPVTVP----ASLPADFQDFLHKCVCLNDAERWTTQQLLDHSFLK-PPLPKNLLHYQETSPEDLAEDFASTVIPQNHILNSPFSSGLQRQFSRYFNEFEELQLLGKGAFGAVIKVQNKLDGCYYAVKRIQVNPASKQFRRIKGEVTLLSRLNHENIVRYYNAWIERHELPST---GIPSNSDSSEAQSPADQHPKSRASPQRMNELGLPDNVEDMAPPPALSSSVEWSTSIEKSSSAKCSGHQSSDEDEDEDDEDVFGASFLPSDSDSRS-----DIIF---DNGD---------ESADEMSQAEKSERPAT----DTTESLDSDRQLSIAHYLYIQMEYCEK--STLRDTI-DQSLHLDQNRLWRLFREILDGLAYIHEQGMIHRDLKPVNIFLDSQDHVKIGDFGLATDHPANV--AAGKFELEDSGSAAVLKPDPTGNMTGMVGTALYVSPEVQGNTKATYNQKVDLFSLGIILFEMSYRPMTTGAERISVLGKLRVEPISLPEDFPAYNQGTQRKVIEWLLKHDPALRPTAQELLKSELLPPPQMEESELHEVLQHTMANINGKAYRTMVGQLFAQNASPVMDYTYDI---------------------------DLHKGNFSVNTAKLQQHVYETITRIFKKHGAVRLQTPLLLPRNRKLYDGSELACF--MDHSGMLVTLPYDLRMAFARFVARNNIAHLKRYSIERVFRPRKLDRAHPRELVECAFDIVTPVTNSLLPDAEIIYTISEIIQEFSALQERNYNIYLNHTSLLKAIL 1096

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Medaka
Match: EIF2AK4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:NCBI gene;Acc:101172779])

HSP 1 Score: 361.688 bits (927), Expect = 2.065e-101
Identity = 304/1035 (29.37%), Postives = 494/1035 (47.73%), Query Frame = 1

Query:  703 LVYKGICDGQHRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQIR----------FSDLKRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDN---ALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKRF-----EELKGRGHSNERTGASTIKKGSKRYDLMKLGVL------AKELFFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFLTCENLSKN--SRQNSQTVDSAQDL------------TPWH--LFEKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSS-AISVIKPETTDSLLL--DFQDKK------NSIMFSANVNFSQIEHCSRHFDLESFXXXXXXXXXXXXVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPFHGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGP-------VGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFT--LKRFVTRYIESIFVSHGAVEFLAPLLSPK----FINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDY-DSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALM 3591
            +V+KG C G+        + ++  + +  F    G    +Y+            F+  ++ + E+ K   +   E +F  L +L+H +L+ Y        E  +  T H  L++++V                  PG+  N      + + + K+ +Y   +L A+ YLH  ++    +   S+L+D +  +++ ++ + KRF     E++  + H     G     K  K+ D+  LG++       KE+  + +         +P+D  DF+  C           ++L  H FL    L KN    Q +   D A+D             +P+   L  + SR   EFE +  LG+GAFG V+KV+N  D  YYA+KR+++N   K+  R   EV LLS LNHENIVRY+ +W E  +  S+    +  N DSS  ++       S   P+  + L L  + +D        +S+ +S ++  S    CS H   +   D D +    +S    D   R      +I F   DN D          + DE+S+  KS R  +    D+  +     +  I  YL IQME CE   +TL+  I DQ L++++  +WR  REI+ GL +IH + MIHRDLKP NIFLDSQ  VKIGD GLA +    V  +A +  ++D+G +  ++  P       VGT +Y+ PE+    +++Y  K D++SLGII+FEM ++P  TG+ER  +LG LR   I  P++     + +  ++I+ LL HD  LRP+A ELL+S ++PPP+ +E EL + L     N +   Y+ M+ +LF++  S +  Y   +                            + +G+F   T  L++ V   I  IF  HGAV    PLL P+    +  +E   F  +D  G++V LP +LRM FA F+  N I+  KRY+I  +++ + + R HPRE +E AFDIVT   +S    AEI+Y + +I ++    +     + +N+T+L  A++
Sbjct:  270 VVHKGKCLGE--------SQRLGRSVYCAFEANSGEFSVLYEWSLRWSKKMGKFFTSQEKGKIENCKKQ-IHAAENEFNSLLRLSHQNLVHYMALSSIEKEDCL--TVH--LLVEHV------------------PGTSLNQSLGADSPVLLDKLCHYAAQLLSALDYLHTNSVVHKHLGASSVLVDSEGTVRLTDYSLSKRFSDICKEDIFEQAHVRFSEGVVMPTKTGKKGDVWNLGLMLLALSQGKEVMEYPV----TVPASLPADFQDFLHKCVCLNDAERWTTQQLLDHSFLK-PPLPKNLLHYQETSPEDLAEDFASTVIPQNHILNSPFSSGLQRQFSRYFNEFEELQLLGKGAFGAVIKVQNKLDGCYYAVKRIQVNPASKQFRRIKGEVTLLSRLNHENIVRYYNAWIERHELPST---GIPSNSDSSEAQSPADQHPKSRASPQRMNELGLPDNVEDMAPPPALSSSVEWSTSIEKSSSAKCSGHQSSDEDEDEDDEDVFGASFLPSDSDSRS-----DIIF---DNGD---------ESADEMSQAEKSERPAT----DTTESLDSDRQLSIAHYLYIQMEYCEK--STLRDTI-DQSLHLDQNRLWRLFREILDGLAYIHEQGMIHRDLKPVNIFLDSQDHVKIGDFGLATDHPANV--AAGKFELEDSGSAAVLKPDPTGNMTGMVGTALYVSPEVQGNTKATYNQKVDLFSLGIILFEMSYRPMTTGAERISVLGKLRVEPISLPEDFPAYNQGTQRKVIEWLLKHDPALRPTAQELLKSELLPPPQMEESELHEVLQHTMANINGKAYRTMVGQLFAQNASPVMDYTYDI---------------------------DLHKGNFSVNTAKLQQHVYETITRIFKKHGAVRLQTPLLLPRNRKLYDGSELACF--MDHSGMLVTLPYDLRMAFARFVARNNIAHLKRYSIERVFRPRKLDRAHPRELVECAFDIVTPVTNSLLPDAEIIYTISEIIQEFSALQERNYNIYLNHTSLLKAIL 1210

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Medaka
Match: EIF2AK4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:NCBI gene;Acc:101172779])

HSP 1 Score: 268.085 bits (684), Expect = 5.089e-72
Identity = 179/508 (35.24%), Postives = 275/508 (54.13%), Query Frame = 1

Query: 2125 SFGYSDND---DNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPFHGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGP-------VGTIMYICPEMLN--QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFT--LKRFVTRYIESIFVSHGAVEFLAPLLSPK----FINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDY-DSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALM 3591
            SF  SD+D   D   DN     + DE+S+  KS R  +    D+  +     +  I  YL IQME CE   +TL+  ID Q L++++  +WR  REI+ GL +IH + MIHRDLKP NIFLDSQ  VKIGD GLA +    V  +A +  ++D+G +  ++  P       VGT +Y+ PE+    +++Y  K D++SLGII+FEM ++P  TG+ER  +LG LR   I  P++     + +  ++I+ LL HD  LRP+A ELL+S ++PPP+ +E EL + L     N +   Y+ M+ +LF++  S +  Y   +                            + +G+F   T  L++ V   I  IF  HGAV    PLL P+    +  +E   F  +D  G++V LP +LRM FA F+  N I+  KRY+I  +++ + + R HPRE +E AFDIVT   +S    AEI+Y + +I ++    +     + +N+T+L  A++
Sbjct:  705 SFLPSDSDSRSDIIFDN--GDESADEMSQAEKSERPAT----DTTESLDSDRQLSIAHYLYIQMEYCEK--STLRDTID-QSLHLDQNRLWRLFREILDGLAYIHEQGMIHRDLKPVNIFLDSQDHVKIGDFGLATDHPANV--AAGKFELEDSGSAAVLKPDPTGNMTGMVGTALYVSPEVQGNTKATYNQKVDLFSLGIILFEMSYRPMTTGAERISVLGKLRVEPISLPEDFPAYNQGTQRKVIEWLLKHDPALRPTAQELLKSELLPPPQMEESELHEVLQHTMANINGKAYRTMVGQLFAQNASPVMDYTYDI---------------------------DLHKGNFSVNTAKLQQHVYETITRIFKKHGAVRLQTPLLLPRNRKLYDGSELACF--MDHSGMLVTLPYDLRMAFARFVARNNIAHLKRYSIERVFRPRKLDRAHPRELVECAFDIVTPVTNSLLPDAEIIYTISEIIQEFSALQERNYNIYLNHTSLLKAIL 1172

HSP 2 Score: 80.8777 bits (198), Expect = 1.303e-14
Identity = 89/385 (23.12%), Postives = 163/385 (42.34%), Query Frame = 1

Query:  703 LVYKGICDGQHRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQIR----------FSDLKRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDN---ALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKRF-----EELKGRGHSNERTGASTIKKGSKRYDLMKLGVL------AKELFFFGIPETEREETDMPSDLIDFITSC-------NSNIEELKLHPFLTCENLSKN--SRQNSQTVDSAQDL------------TPWH--LFEKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLN 1716
            +V+KG C G+        + ++  + +  F    G    +Y+            F+  ++ + E+ K   +   E +F  L +L+H +L+ Y        E  +  T H  L++++V                  PG+  N      + + + K+ +Y   +L A+ YLH  ++    +   S+L+D +  +++ ++ + KRF     E++  + H     G     K  K+ D+  LG++       KE+  + +         +P+D  DF+  C           ++L  H FL    L KN    Q +   D A+D             +P+   L  + SR   EFE +  LG+GAFG V+KV+N  D  YYA+KR+++N
Sbjct:  270 VVHKGKCLGE--------SQRLGRSVYCAFEANSGEFSVLYEWSLRWSKKMGKFFTSQEKGKIENCKKQ-IHAAENEFNSLLRLSHQNLVHYMALSSIEKEDCL--TVH--LLVEHV------------------PGTSLNQSLGADSPVLLDKLCHYAAQLLSALDYLHTNSVVHKHLGASSVLVDSEGTVRLTDYSLSKRFSDICKEDIFEQAHVRFSEGVVMPTKTGKKGDVWNLGLMLLALSQGKEVMEYPV----TVPASLPADFQDFLHKCVCLNDAERWTTQQLLDHSFLK-PPLPKNLLHYQETSPEDLAEDFASTVIPQNHILNSPFSSGLQRQFSRYFNEFEELQLLGKGAFGAVIKVQNKLDGCYYAVKRIQVN 618

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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Medaka
Match: eif2ak1 (eukaryotic translation initiation factor 2-alpha kinase 1 [Source:NCBI gene;Acc:101174538])

HSP 1 Score: 115.546 bits (288), Expect = 9.980e-26
Identity = 77/218 (35.32%), Postives = 120/218 (55.05%), Query Frame = 1

Query: 2293 LMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWR-------------FIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQK-RVKIGDLGLAMNFNNKVTESANQEYVKDNGLSND-VENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKH--FKIKSSFVELIKSLLNHDQLLRPSASELLQSNI 2895
            L IQM+LC+    +L+ +I ++  +  E+ I R              +R ++ G+E+IH+K ++HRDLKP NIFL      V+IGD GLA        +     Y + N   +D +    VGT +Y  PE L  S Y +K+DMYS+G+I  E+  +PFGT  ER   LG+LR  ++  PD  H  + + S +   IK+L + +  +R +A++LLQS +
Sbjct:  369 LYIQMQLCD---RSLKDWISERNSWSKEEDISRGHYDCVDAEHTLSLLRHVLEGVEYIHAKGIMHRDLKPRNIFLQGPDCHVRIGDFGLA------CRDILVDGYKRSNSSGSDSIHTSGVGTFVYASPEQLKGSHYDSKSDMYSIGVIALEL-FQPFGTEMERVRTLGDLREGKV--PDSFHQRWPVLSKY---IKNLTSKEPSVRLTATQLLQSEL 571

HSP 2 Score: 80.1073 bits (196), Expect = 1.169e-14
Identity = 35/82 (42.68%), Postives = 59/82 (71.95%), Query Frame = 1

Query: 1588 KSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKK-AARTMKEVQLLSSLNHENIVRYFASWSEEMQKS 1830
            ++SR   EFE IC+LG+G++G+V KVRN  D Q+YA+K++ +  + K+   + ++EV++LSSL+H N+V Y  +W E +Q +
Sbjct:  144 QTSRYLSEFEEICRLGKGSYGNVFKVRNKLDAQFYAVKKILIKKVSKEDCMKVLREVKVLSSLHHVNVVGYHTAWMEHVQPA 225

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Medaka
Match: eif2ak1 (eukaryotic translation initiation factor 2-alpha kinase 1 [Source:NCBI gene;Acc:101174538])

HSP 1 Score: 115.546 bits (288), Expect = 1.170e-25
Identity = 77/218 (35.32%), Postives = 120/218 (55.05%), Query Frame = 1

Query: 2293 LMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWR-------------FIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQK-RVKIGDLGLAMNFNNKVTESANQEYVKDNGLSND-VENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKH--FKIKSSFVELIKSLLNHDQLLRPSASELLQSNI 2895
            L IQM+LC+    +L+ +I ++  +  E+ I R              +R ++ G+E+IH+K ++HRDLKP NIFL      V+IGD GLA        +     Y + N   +D +    VGT +Y  PE L  S Y +K+DMYS+G+I  E+  +PFGT  ER   LG+LR  ++  PD  H  + + S +   IK+L + +  +R +A++LLQS +
Sbjct:  404 LYIQMQLCD---RSLKDWISERNSWSKEEDISRGHYDCVDAEHTLSLLRHVLEGVEYIHAKGIMHRDLKPRNIFLQGPDCHVRIGDFGLA------CRDILVDGYKRSNSSGSDSIHTSGVGTFVYASPEQLKGSHYDSKSDMYSIGVIALEL-FQPFGTEMERVRTLGDLREGKV--PDSFHQRWPVLSKY---IKNLTSKEPSVRLTATQLLQSEL 606

HSP 2 Score: 80.1073 bits (196), Expect = 1.140e-14
Identity = 35/82 (42.68%), Postives = 59/82 (71.95%), Query Frame = 1

Query: 1588 KSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKK-AARTMKEVQLLSSLNHENIVRYFASWSEEMQKS 1830
            ++SR   EFE IC+LG+G++G+V KVRN  D Q+YA+K++ +  + K+   + ++EV++LSSL+H N+V Y  +W E +Q +
Sbjct:  179 QTSRYLSEFEEICRLGKGSYGNVFKVRNKLDAQFYAVKKILIKKVSKEDCMKVLREVKVLSSLHHVNVVGYHTAWMEHVQPA 260

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Planmine SMEST
Match: SMESG000023579.1 (SMESG000023579.1)

HSP 1 Score: 3385.5 bits (8777), Expect = 0.000e+0
Identity = 1689/1699 (99.41%), Postives = 1691/1699 (99.53%), Query Frame = 1

Query:   97 MSSYDVTILQSDEVKALRSIFGKEFKLIRPKNLKKKYEPPLVSVLINVDSSNLNSALSLRISFDCPQNYPFDIPKISIYDAVNVPTNLVEQLLIILNEKSNLLKGYSMIYNLVEEARCIIIKHFETDHKSVNSILTINKVLNEDEIMQTETKQSEFLKNLLRQRRDSARXXXXXXXXXXXCDHSYKTDELEFEHCSDRSSGCLVYKGICDGQHRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQIRFSDLKRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKRFEELKGRGHSNERTGASTIKKGSKRYDLMKLGVLAKELFFFGIPETEREETDMPSDLIDFITSCNSNIEELKLHPFLTCENLSKNSRQNSQTVDSAQDLTPWHLFEKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSSAISVIKPETTDSLLLDFQDKKNSIMFSANVNFSQIEHCSRHFDLESFXXXXXXXXXXX----XVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPFHGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPKFINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDYDSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRASLHDMVSKDVKNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNRIHEGYDVINSAFKELKSVEEKLKHFTEDLCFCVNFRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEIYDKDEHGEEYVFNQKIHFYVRFPSRYEEKCYSDARISVKRNDWKGLGLEQISNDNVKKPANVVNLILREMKKSKENLFNRFEKEPYQRQTSDSLSADSCTDFDSKKINNLKTETNPTRYIYKFITAKESSKFGSQEFTIPYFVESNKQMQLLNQKTPNLELPQIQMIRKVNCQLLNVNKWNQGKFPNPLYCLICDISHSKCXXXXXXXXXXXXXXTNRLTKLFNEIESSRNYQNNVEKQWKDVLARIWESLQNAVKEKLFRIVIFSQIDENFVFLEIK 5181
            MSSYDVTILQSDEVKALRSIFGKEFKLIRPKNLKKKYEPPLVSVLINVDSSNLNSALSLRISFDCPQNYPFDIPKISIYDAVNVPTNLVEQLLIILNEKSNLLKGYSMIYNLVEEARCIIIKHFETDHKSVNSILTINKVLNEDEIMQTETKQSEFLKNLLRQRRDSARNEKKNEKKFEKCDHSYKTDELEFEHCSDRSSGCLVYKGICDGQHRIKTIN NMQIKLNTFNGFNRQKGTNVKIYQIRFSDLKRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKRFEELKGRGHSNERTGASTIKKGSKRYDLMKLGVLAKELFFFGIPETEREETDMPSDLIDFITSCNSNIEELKLHPFLTCENLSKNSRQNSQTVDSAQDLTPWHLFEKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSSAISVIKPETTDSLLLDFQDKKNSIMFSANVNFSQIEHCSRHFDLESFSDSDSDSDSDS    SVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNP HGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPKFINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDYDSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRASLHDMVSKD+KNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNR HEGYDVINSAFKELKSVEEKLKHFTEDLCFCV FRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEIYDKDEHGEEYVFNQKIHFYVRFPSRYEEKCYSDARISVKRNDWKGLGLEQISNDNVKKPANVV+LILREMKKSKENLFNRFEKEPYQRQTSDSLSADSCTDFDSKKINNLKTETNPTRYIYKFITAKESSKFGSQEFTIPYFVESNKQMQLLNQKTPNLELPQIQMIRKVNCQLLNVNKWNQGKFPNPLYCLICDISHSKCLSLVEEVSLKSLKETNRLTKLFNEIESSRNYQNNVEKQWKDVLARIWESLQNAVKEKLFRIVIFSQIDENFVFLEIK
Sbjct:    1 MSSYDVTILQSDEVKALRSIFGKEFKLIRPKNLKKKYEPPLVSVLINVDSSNLNSALSLRISFDCPQNYPFDIPKISIYDAVNVPTNLVEQLLIILNEKSNLLKGYSMIYNLVEEARCIIIKHFETDHKSVNSILTINKVLNEDEIMQTETKQSEFLKNLLRQRRDSARNEKKNEKKFEKCDHSYKTDELEFEHCSDRSSGCLVYKGICDGQHRIKTINLNMQIKLNTFNGFNRQKGTNVKIYQIRFSDLKRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKRFEELKGRGHSNERTGASTIKKGSKRYDLMKLGVLAKELFFFGIPETEREETDMPSDLIDFITSCNSNIEELKLHPFLTCENLSKNSRQNSQTVDSAQDLTPWHLFEKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSSAISVIKPETTDSLLLDFQDKKNSIMFSANVNFSQIEHCSRHFDLESFSDSDSDSDSDSDSDSSVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPSHGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPKFINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDYDSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRASLHDMVSKDIKNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNRNHEGYDVINSAFKELKSVEEKLKHFTEDLCFCVKFRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEIYDKDEHGEEYVFNQKIHFYVRFPSRYEEKCYSDARISVKRNDWKGLGLEQISNDNVKKPANVVDLILREMKKSKENLFNRFEKEPYQRQTSDSLSADSCTDFDSKKINNLKTETNPTRYIYKFITAKESSKFGSQEFTIPYFVESNKQMQLLNQKTPNLELPQIQMIRKVNCQLLNVNKWNQGKFPNPLYCLICDISHSKCLSLVEEVSLKSLKETNRLTKLFNEIESSRNYQNNVEKQWKDVLARIWESLQNAVKEKLFRIVIFSQIDENFVFLEIK 1699

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Planmine SMEST
Match: SMESG000023579.1 (SMESG000023579.1)

HSP 1 Score: 3380.88 bits (8765), Expect = 0.000e+0
Identity = 1689/1700 (99.35%), Postives = 1691/1700 (99.47%), Query Frame = 1

Query:   97 MSSYDVTILQSDEVKALRSIFGKEFKLIRPKNLKKKYEPPLVSVLINVDSSNLNSALSLRISFDCPQNYPFDIPKISIYDAVNVPTNLVEQLLIILNEKSNLLKGYSMIYNLVEEARCIIIKHFETDHKSVNSILTINKVLNEDEIMQTETKQSEFLKNLLRQRRDSARXXXXXXXXXXXCDHSYKTDELEFEHCSDRSSGCLVYKGICDGQHRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQIRFSDLKRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKRFEELKGRGHSNERTGASTIKKGSKRYDLMKLGVLAKELFFFGIPETEREETDMPSDLIDFITSCNSNIEELKLHPFLTCENLSKNSRQNSQTVDSAQ-DLTPWHLFEKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSSAISVIKPETTDSLLLDFQDKKNSIMFSANVNFSQIEHCSRHFDLESFXXXXXXXXXXX----XVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPFHGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPKFINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDYDSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRASLHDMVSKDVKNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNRIHEGYDVINSAFKELKSVEEKLKHFTEDLCFCVNFRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEIYDKDEHGEEYVFNQKIHFYVRFPSRYEEKCYSDARISVKRNDWKGLGLEQISNDNVKKPANVVNLILREMKKSKENLFNRFEKEPYQRQTSDSLSADSCTDFDSKKINNLKTETNPTRYIYKFITAKESSKFGSQEFTIPYFVESNKQMQLLNQKTPNLELPQIQMIRKVNCQLLNVNKWNQGKFPNPLYCLICDISHSKCXXXXXXXXXXXXXXTNRLTKLFNEIESSRNYQNNVEKQWKDVLARIWESLQNAVKEKLFRIVIFSQIDENFVFLEIK 5181
            MSSYDVTILQSDEVKALRSIFGKEFKLIRPKNLKKKYEPPLVSVLINVDSSNLNSALSLRISFDCPQNYPFDIPKISIYDAVNVPTNLVEQLLIILNEKSNLLKGYSMIYNLVEEARCIIIKHFETDHKSVNSILTINKVLNEDEIMQTETKQSEFLKNLLRQRRDSARNEKKNEKKFEKCDHSYKTDELEFEHCSDRSSGCLVYKGICDGQHRIKTIN NMQIKLNTFNGFNRQKGTNVKIYQIRFSDLKRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKRFEELKGRGHSNERTGASTIKKGSKRYDLMKLGVLAKELFFFGIPETEREETDMPSDLIDFITSCNSNIEELKLHPFLTCENLSKNSRQNSQTVDSAQ DLTPWHLFEKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSSAISVIKPETTDSLLLDFQDKKNSIMFSANVNFSQIEHCSRHFDLESFSDSDSDSDSDS    SVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNP HGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPKFINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDYDSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRASLHDMVSKD+KNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNR HEGYDVINSAFKELKSVEEKLKHFTEDLCFCV FRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEIYDKDEHGEEYVFNQKIHFYVRFPSRYEEKCYSDARISVKRNDWKGLGLEQISNDNVKKPANVV+LILREMKKSKENLFNRFEKEPYQRQTSDSLSADSCTDFDSKKINNLKTETNPTRYIYKFITAKESSKFGSQEFTIPYFVESNKQMQLLNQKTPNLELPQIQMIRKVNCQLLNVNKWNQGKFPNPLYCLICDISHSKCLSLVEEVSLKSLKETNRLTKLFNEIESSRNYQNNVEKQWKDVLARIWESLQNAVKEKLFRIVIFSQIDENFVFLEIK
Sbjct:    1 MSSYDVTILQSDEVKALRSIFGKEFKLIRPKNLKKKYEPPLVSVLINVDSSNLNSALSLRISFDCPQNYPFDIPKISIYDAVNVPTNLVEQLLIILNEKSNLLKGYSMIYNLVEEARCIIIKHFETDHKSVNSILTINKVLNEDEIMQTETKQSEFLKNLLRQRRDSARNEKKNEKKFEKCDHSYKTDELEFEHCSDRSSGCLVYKGICDGQHRIKTINLNMQIKLNTFNGFNRQKGTNVKIYQIRFSDLKRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTKHFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKSILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKRFEELKGRGHSNERTGASTIKKGSKRYDLMKLGVLAKELFFFGIPETEREETDMPSDLIDFITSCNSNIEELKLHPFLTCENLSKNSRQNSQTVDSAQGDLTPWHLFEKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSSAISVIKPETTDSLLLDFQDKKNSIMFSANVNFSQIEHCSRHFDLESFSDSDSDSDSDSDSDSSVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPSHGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKHFKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLIDVTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVFRNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPKFINTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQQQAIRRDHPRERIEAAFDIVTDYDSPNKTAEILYLLHQIFKKSFNSEHATLTLEINNTNLFYALMNHYSIRASLHDMVSKDIKNLDSDHFRSLTNLTDKQKANIKSILLFNGSRLEDLEKHFKIFQNRNHEGYDVINSAFKELKSVEEKLKHFTEDLCFCVKFRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDKNMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASIDVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGIIWDLVSRLWAANISAEIYDKDEHGEEYVFNQKIHFYVRFPSRYEEKCYSDARISVKRNDWKGLGLEQISNDNVKKPANVVDLILREMKKSKENLFNRFEKEPYQRQTSDSLSADSCTDFDSKKINNLKTETNPTRYIYKFITAKESSKFGSQEFTIPYFVESNKQMQLLNQKTPNLELPQIQMIRKVNCQLLNVNKWNQGKFPNPLYCLICDISHSKCLSLVEEVSLKSLKETNRLTKLFNEIESSRNYQNNVEKQWKDVLARIWESLQNAVKEKLFRIVIFSQIDENFVFLEIK 1700

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Planmine SMEST
Match: SMESG000020439.1 (SMESG000020439.1)

HSP 1 Score: 120.553 bits (301), Expect = 9.309e-28
Identity = 128/448 (28.57%), Postives = 207/448 (46.21%), Query Frame = 1

Query: 1513 LSKNSRQNSQTVDSAQDLTPWHLFEKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIK---KKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKS------------SSETNNLQPNIDSSSIRNNTSSAISVIKPETTDSLLLDFQDKKNSIMFSANVNFSQIEHCSRHFDLESFXXXXXXXXXXXXVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPFHGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNE------KLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFL----DSQKRVKIGDLGLAMNFN-NKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLN----QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRR 2766
            +S N  QNS  +   Q+  P     K +++  +F+ I  +G+G FG V    N  D Q YAIK+V +N  K   K+ A T+KE ++L+ LNH NIVRY   W E  +++            S    N  P I  SS   N SS I       + S+  +  +  N    SA  N   + H                    S+++ ++   R+   +  I F YS N ++  D+    ++ D+ + ++     KS  +N            K+E  L IQME C   +  LQ ++ ++   ++       K +    R+++ G+E+IHS N+IHRD+KP NIFL        ++KIGD GL+  F  +K  E ++   +K             GT  Y  PE+ N    + SY +K+D+YS+G+I+ E+  K F T SE  H++ ++ +
Sbjct:   54 ISPNFIQNS--ISFVQNFKPIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYANSIPRISHSSSFTNNSSVI------CSTSMSFNGSNVSNLTNNSA-FNTGSLSH---------------SICDPSNIDTRNSDCRHSDSN--IIFEYSSNFNHKHDSLTSHSSEDKGALEI----CKSSNVN----------VPKMEFALYIQMEFC---HLNLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMK--------LTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVK-FSTQSELCHVVEDISK 449

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Planmine SMEST
Match: SMESG000020439.1 (SMESG000020439.1)

HSP 1 Score: 120.553 bits (301), Expect = 1.023e-27
Identity = 128/448 (28.57%), Postives = 207/448 (46.21%), Query Frame = 1

Query: 1513 LSKNSRQNSQTVDSAQDLTPWHLFEKSSRIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIK---KKAARTMKEVQLLSSLNHENIVRYFASWSEEMQKS------------SSETNNLQPNIDSSSIRNNTSSAISVIKPETTDSLLLDFQDKKNSIMFSANVNFSQIEHCSRHFDLESFXXXXXXXXXXXXVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDELSKQVKSMRLKSGYINDSQNNPFHGNKTKIEKYLMIQMELCEGQYNTLQKYIDDQKLYMNE------KLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFL----DSQKRVKIGDLGLAMNFN-NKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLN----QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRR 2766
            +S N  QNS  +   Q+  P     K +++  +F+ I  +G+G FG V    N  D Q YAIK+V +N  K   K+ A T+KE ++L+ LNH NIVRY   W E  +++            S    N  P I  SS   N SS I       + S+  +  +  N    SA  N   + H                    S+++ ++   R+   +  I F YS N ++  D+    ++ D+ + ++     KS  +N            K+E  L IQME C   +  LQ ++ ++   ++       K +    R+++ G+E+IHS N+IHRD+KP NIFL        ++KIGD GL+  F  +K  E ++   +K             GT  Y  PE+ N    + SY +K+D+YS+G+I+ E+  K F T SE  H++ ++ +
Sbjct:   66 ISPNFIQNS--ISFVQNFKPIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYANSIPRISHSSSFTNNSSVI------CSTSMSFNGSNVSNLTNNSA-FNTGSLSH---------------SICDPSNIDTRNSDCRHSDSN--IIFEYSSNFNHKHDSLTSHSSEDKGALEI----CKSSNVN----------VPKMEFALYIQMEFC---HLNLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMK--------LTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVK-FSTQSELCHVVEDISK 461

[Close]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Planmine SMEST
Match: SMESG000055481.1 (SMESG000055481.1)

HSP 1 Score: 93.9745 bits (232), Expect = 3.075e-20
Identity = 50/146 (34.25%), Postives = 87/146 (59.59%), Query Frame = 1

Query: 2293 LMIQMELCE-GQYNTLQKYIDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRVKIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLNQSSYTNKADMYSLGIIVFEMC--HKPF 2721
            L+I +EL + G  + + K+ + QK  + E+ IW++  +I S LE++HS+ ++HRD+KP N+F+ S+  VK+GD GL   F+   T+++N   +             VGT  Y+ PE + ++ Y  K+D++SLG +++EM     PF
Sbjct:   96 LIIVLELADAGDLSRMIKHFNKQKRLIPERTIWKYFVQICSALEYMHSQRIMHRDIKPANVFITSKGEVKLGDFGLGRFFS---TQTSNVHSL-------------VGTPYYMSPERILETGYDMKSDIWSLGCVLYEMAALQSPF 225

The following BLAST results are available for this feature:

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Human
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Celegans
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Fly
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Zebrafish
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Xenopus
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Mouse
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. UniProt/SwissProt
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. TrEMBL
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Cavefish
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Sea Lamprey
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Yeast
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Nematostella
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Medaka
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Planmine SMEST

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
EIF2AK4	2.263e-95	25.47	eukaryotic translation initiation factor 2 alpha k...	[more]
EIF2AK4	5.367e-66	31.01	eukaryotic translation initiation factor 2 alpha k...	[more]
EIF2AK1	9.579e-40	27.12	eukaryotic translation initiation factor 2 alpha k...	[more]
NEK3	2.808e-21	30.67	NIMA related kinase 3 [Source:HGNC Symbol;Acc:HGNC...	[more]
NEK6	8.651e-21	33.09	NIMA related kinase 6 [Source:HGNC Symbol;Acc:HGNC...	[more]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
gcn-2	3.219e-41	24.77	Eukaryotic translation initiation factor 2-alpha k...	[more]
gcn-2	3.219e-41	24.77	Eukaryotic translation initiation factor 2-alpha k...	[more]
gcn-2	3.219e-41	24.77	Eukaryotic translation initiation factor 2-alpha k...	[more]
gcn-2	3.297e-41	24.77	Eukaryotic translation initiation factor 2-alpha k...	[more]
gcn-2	3.297e-41	24.77	Eukaryotic translation initiation factor 2-alpha k...	[more]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Gcn2	7.024e-56	29.79	gene:FBgn0019990 transcript:FBtr0334552	[more]
Gcn2	7.130e-56	29.79	gene:FBgn0019990 transcript:FBtr0334551	[more]
Gcn2	7.130e-56	29.79	gene:FBgn0019990 transcript:FBtr0085787	[more]
PEK	5.834e-20	32.59	gene:FBgn0037327 transcript:FBtr0078770	[more]
PEK	7.014e-20	32.59	gene:FBgn0037327 transcript:FBtr0330026	[more]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
EIF2AK4	4.331e-99	28.54	eukaryotic translation initiation factor 2 alpha k...	[more]
eif2ak1	2.211e-46	31.00	eukaryotic translation initiation factor 2-alpha k...	[more]
eif2ak1	5.971e-46	30.75	eukaryotic translation initiation factor 2-alpha k...	[more]
eif2ak1	1.621e-45	30.75	eukaryotic translation initiation factor 2-alpha k...	[more]
pkz	1.304e-22	36.14	protein kinase containing Z-DNA binding domains [S...	[more]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
eif2ak4	7.288e-108	28.47	eukaryotic translation initiation factor 2 alpha k...	[more]
eif2ak4	9.074e-108	28.47	eukaryotic translation initiation factor 2 alpha k...	[more]
eif2ak4	2.148e-106	28.16	eukaryotic translation initiation factor 2 alpha k...	[more]
eif2ak4	4.160e-76	31.56	eukaryotic translation initiation factor 2 alpha k...	[more]
eif2ak4	6.484e-76	31.56	eukaryotic translation initiation factor 2 alpha k...	[more]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Eif2ak4	1.760e-74	31.52	eukaryotic translation initiation factor 2 alpha k...	[more]
Eif2ak4	4.012e-74	31.52	eukaryotic translation initiation factor 2 alpha k...	[more]
Eif2ak4	4.759e-74	31.52	eukaryotic translation initiation factor 2 alpha k...	[more]
Eif2ak4	5.545e-74	31.52	eukaryotic translation initiation factor 2 alpha k...	[more]
Eif2ak4	7.408e-74	31.52	eukaryotic translation initiation factor 2 alpha k...	[more]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q9P2K8\|E2AK4_HUMAN	7.315e-90	27.15	eIF-2-alpha kinase GCN2 OS=Homo sapiens OX=9606 GN...	[more]
sp\|Q9QZ05\|E2AK4_MOUSE	3.770e-73	31.52	eIF-2-alpha kinase GCN2 OS=Mus musculus OX=10090 G...	[more]
sp\|D4A7V9\|E2AK4_RAT	6.272e-73	30.85	eIF-2-alpha kinase GCN2 OS=Rattus norvegicus OX=10...	[more]
sp\|Q9HGN1\|GCN2_SCHPO	4.775e-58	29.81	eIF-2-alpha kinase GCN2 OS=Schizosaccharomyces pom...	[more]
sp\|P15442\|GCN2_YEAST	3.804e-42	27.18	eIF-2-alpha kinase GCN2 OS=Saccharomyces cerevisia...	[more]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A3Q2X7J9	3.620e-108	27.00	Eukaryotic translation initiation factor 2 alpha k...	[more]
A0A3Q2X7G1	5.369e-108	28.74	Eukaryotic translation initiation factor 2 alpha k...	[more]
A0A3Q3C620	5.220e-107	28.74	Eukaryotic translation initiation factor 2 alpha k...	[more]
A0A2I4BIC2	8.872e-107	28.02	eukaryotic translation initiation factor 2-alpha k...	[more]
A0A3P8NFA2	1.426e-106	28.72	Eukaryotic translation initiation factor 2 alpha k...	[more]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
EIF2AK4	3.036e-98	27.77	eukaryotic translation initiation factor 2 alpha k...	[more]
EIF2AK4	4.304e-90	28.38	eukaryotic translation initiation factor 2 alpha k...	[more]
eif2ak1	6.533e-44	30.63	eukaryotic translation initiation factor 2 alpha k...	[more]
eif2ak1	3.068e-42	29.74	eukaryotic translation initiation factor 2 alpha k...	[more]
eif2ak1	6.778e-42	30.15	eukaryotic translation initiation factor 2 alpha k...	[more]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSPMAT00000007738.1	1.809e-99	27.42	pep scaffold:Pmarinus_7.0:GL476507:25231:69652:1 g...	[more]
eif2ak1	1.035e-24	31.46	eukaryotic translation initiation factor 2-alpha k...	[more]
ENSPMAT00000006924.1	3.817e-22	33.95	pep scaffold:Pmarinus_7.0:GL476537:325917:352372:-...	[more]
nek2	4.556e-19	28.97	NIMA-related kinase 2 [Source:ZFIN;Acc:ZDB-GENE-04...	[more]
ENSPMAT00000007414.1	7.072e-18	28.29	pep scaffold:Pmarinus_7.0:GL476981:230882:244405:1...	[more]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5


Match Name	E-value	Identity	Description
GCN2	5.479e-44	27.18	Protein kinase; phosphorylates the alpha-subunit o...	[more]
SSK22	1.621e-16	28.57	MAP kinase kinase kinase of HOG1 mitogen-activated...	[more]
CLA4	1.935e-15	32.64	Cdc42p-activated signal transducing kinase; member...	[more]
KIN82	3.538e-15	29.91	Putative serine/threonine protein kinase; implicat...	[more]
STE11	4.439e-15	33.71	Signal transducing MEK kinase; involved in pheromo...	[more]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO48785	9.270e-23	32.85	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO37413	1.239e-22	32.85	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO46416	1.214e-21	37.13	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO49690	2.681e-21	33.17	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO34646	2.665e-20	32.88	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
EIF2AK4	1.148e-101	29.37	eukaryotic translation initiation factor 2 alpha k...	[more]
EIF2AK4	2.065e-101	29.37	eukaryotic translation initiation factor 2 alpha k...	[more]
EIF2AK4	5.089e-72	35.24	eukaryotic translation initiation factor 2 alpha k...	[more]
eif2ak1	9.980e-26	35.32	eukaryotic translation initiation factor 2-alpha k...	[more]
eif2ak1	1.170e-25	35.32	eukaryotic translation initiation factor 2-alpha k...	[more]

BLAST of Eukaryotic translation initiation factor 2-alpha kinase 4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000023579.1	0.000e+0	99.41	SMESG000023579.1	[more]
SMESG000023579.1	0.000e+0	99.35	SMESG000023579.1	[more]
SMESG000020439.1	9.309e-28	28.57	SMESG000020439.1	[more]
SMESG000020439.1	1.023e-27	28.57	SMESG000020439.1	[more]
SMESG000055481.1	3.075e-20	34.25	SMESG000055481.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30032249 ID=SMED30032249|Name=Eukaryotic translation initiation factor 2-alpha kinase 4|organism=Schmidtea mediterranea sexual|type=transcript|length=5198bp
CACTTTTCAAATCCTATTAATTGTATGCTAATATTTTAAAATTTATATTA
TTTATTGAATTGTCGAAATACTTTTTAAAACTAATCTTATCATCCAATGT
CATCCTATGATGTAACAATATTACAATCAGATGAAGTCAAGGCTCTTCGT
AGTATTTTTGGCAAAGAATTTAAACTAATTAGACCTAAAAATCTTAAAAA
GAAATATGAACCTCCATTGGTGTCTGTATTAATAAATGTTGATTCATCAA
ATTTAAACTCCGCGCTATCTTTAAGAATTTCATTTGATTGTCCTCAGAAT
TATCCATTCGATATCCCAAAGATATCAATATATGATGCAGTTAATGTACC
AACAAATTTAGTTGAACAATTATTAATAATATTAAATGAAAAATCTAATC
TGTTGAAAGGTTATTCAATGATTTATAATTTAGTTGAAGAAGCTAGATGT
ATTATAATAAAACATTTCGAAACTGACCATAAATCTGTAAATTCTATTTT
AACAATTAATAAAGTATTGAATGAAGATGAGATAATGCAAACAGAAACAA
AACAATCCGAATTCCTAAAAAATTTATTACGTCAACGGAGGGATTCAGCA
CGAAATGAAAAGAAAAATGAAAAAAAATTCGAGAAATGTGACCATTCTTA
CAAGACTGATGAGCTGGAATTTGAGCATTGCAGTGACAGATCCAGTGGTT
GTTTGGTTTACAAAGGCATCTGTGACGGCCAGCATAGGATTAAAACAATT
AATCCAAATATGCAAATTAAACTGAACACATTTAATGGATTCAATCGTCA
AAAAGGCACCAATGTAAAAATATATCAAATCAGGTTTTCGGATTTGAAAA
GAAAGCAATTTGAAGATCTCAAATCGAATTGGCTATCGAAACTTGAAGAA
GATTTCACTTTGTTATACAAATTAAACCATCCGCATTTGATTACGTATTA
CGGATTTCGATATCAGGCGCACGAGAAAGTTATTCGCAAAACAAAACACT
TTGACCTTGTCATTCAGTACGTCACGTCAAATGATGAGGGGCTGAAGTTA
TGGAAAGTTTGTGAAATGGATACGAAACCGGGGTCTGGCGACAACGCGTT
GAAAACGAGATTATCTATCAGTAAAATAATAAATTATCTGAAAAGTATTC
TCGAAGCGGTAATATACCTCCACGATAAGAATATCACATTCATTAAAATT
AACATGGATTCCATTTTAATTGATAAAGATCTGCAATTGAAGATTTGCAA
TTTCTGTATTGGTAAACGATTCGAGGAACTTAAGGGACGTGGGCACTCGA
ATGAACGGACAGGAGCTTCAACAATTAAAAAGGGTTCAAAACGATATGAT
TTAATGAAACTGGGCGTGTTAGCTAAAGAATTGTTTTTCTTTGGTATACC
AGAAACTGAACGTGAAGAGACTGATATGCCATCTGACCTTATTGACTTCA
TTACCAGTTGTAATTCAAATATTGAGGAATTAAAATTACATCCTTTCTTA
ACATGTGAAAATCTTTCCAAAAATTCACGGCAGAATTCCCAAACTGTTGA
TTCTGCCCAAGATCTAACTCCATGGCATTTATTTGAGAAATCTTCTCGTA
TTCCGAAAGAATTTGAGCTAATCTGTAAATTAGGTGAAGGAGCATTTGGA
GATGTTGTGAAAGTGAGAAACATCTACGACAATCAATACTATGCAATTAA
ACGAGTGAGACTTAATGCCATAAAAAAGAAAGCAGCGAGAACTATGAAAG
AAGTCCAATTGCTGTCCAGTTTAAACCATGAGAACATAGTGAGGTACTTT
GCATCTTGGTCAGAGGAAATGCAAAAAAGTTCTAGTGAAACAAATAATCT
TCAACCAAACATTGACAGTTCCAGTATAAGAAACAACACCAGCAGTGCAA
TTTCTGTTATTAAACCAGAAACAACAGATTCTTTACTTTTGGATTTTCAA
GATAAAAAGAACAGTATAATGTTTAGTGCAAACGTTAATTTTTCTCAAAT
TGAGCATTGCAGTCGACATTTTGATTTGGAGTCCTTCTCAGATTCTGATT
CAGATTCAGATTCGGATTCAAGTGTAGAATTGAAAGACCAATCACCACGG
TATAAACAGCCGTCAGTTGAAATATCATTTGGATACAGTGACAATGACGA
CAATCCATTAGACAATCATCATAAGAAAAATACACCTGATGAACTTTCAA
AACAAGTGAAAAGTATGCGCCTGAAATCGGGATATATAAATGATTCTCAG
AATAACCCCTTTCATGGCAATAAAACCAAAATCGAAAAATATCTTATGAT
TCAAATGGAACTTTGTGAAGGGCAATATAATACCCTCCAAAAATATATAG
ATGATCAGAAATTATACATGAACGAGAAATTAATATGGAGATTCATTCGA
GAAATAGTATCTGGTCTCGAACATATTCATTCTAAAAATATGATTCATCG
GGATCTGAAACCACACAATATATTTCTTGACTCTCAGAAACGAGTGAAGA
TCGGTGACCTAGGATTAGCAATGAACTTCAACAACAAAGTAACCGAGTCT
GCTAATCAAGAATATGTTAAAGACAATGGTTTATCGAATGATGTCGAAAA
TGGTCCTGTGGGAACTATTATGTATATTTGTCCAGAGATGCTCAATCAAT
CAAGCTACACAAATAAAGCTGATATGTACAGTCTAGGAATCATTGTATTT
GAAATGTGTCACAAACCTTTCGGCACTGGGTCTGAACGTTATCATATTCT
TGGAAATTTACGAAGACCTGAAATCATATTTCCCGATGAAAAACATTTTA
AAATAAAGTCATCTTTTGTTGAACTAATTAAATCTCTCCTAAATCACGAC
CAGTTACTTCGTCCCAGTGCATCAGAATTACTCCAGTCAAACATTATTCC
GCCCCCTGAAGGCAAAGAATTGGAATTACGAAAATTCCTCATCGATGTCA
CAAAGAATAGCAGTAGTAATTTGTATAAATTGATGCTGAAAGAACTATTT
AGTAAGTTATATTCTGAGATCTGTCGCTATGAATTGTCATTACTCTACCA
GTCACCGTTCAATCATTTCATTTCTAATACTAAATCTCTTGTCTTTCGAA
ATTTGAGAAATCCGCTGTCCTCCCAAATAGGAGAGGGGGACTTTTTCTTT
TTTACTCTTAAACGATTCGTCACTCGTTATATTGAATCGATTTTCGTATC
TCATGGAGCTGTAGAATTCCTCGCGCCTTTATTATCACCAAAATTTATAA
ATACAGAATCTGGTTTTTTTTCTCTTTTGGACGCTGAAGGAGTTGTCGTT
AATTTACCGAACAATCTCCGAATGAACTTCGCTAATTTTCTCGTCGATAA
TAGGATATCATGCTGTAAACGTTACGCAATCGGGCCGATATATCAACAGC
AAGCCATTCGCAGAGATCATCCAAGAGAACGGATAGAAGCTGCGTTTGAT
ATAGTGACCGATTACGATAGCCCAAATAAAACCGCTGAAATTCTTTATCT
TCTTCATCAAATTTTCAAGAAATCCTTCAATTCTGAGCACGCTACTCTAA
CATTGGAAATCAACAACACAAATTTGTTCTATGCTCTCATGAATCATTAT
AGTATTCGTGCTTCACTGCATGATATGGTATCCAAGGATGTTAAAAATTT
GGACAGTGATCATTTCAGAAGTCTCACAAATCTTACGGACAAACAGAAAG
CAAACATTAAATCCATCCTCTTGTTCAATGGCAGTCGATTAGAAGATTTA
GAAAAGCATTTCAAAATATTTCAAAATCGAATTCATGAAGGCTACGATGT
GATCAATAGCGCATTTAAAGAATTGAAATCTGTTGAAGAGAAACTTAAAC
ATTTCACTGAAGATCTTTGTTTTTGCGTTAATTTTCGACCGAGTCTTGTT
CCACTGGAAACGATTTATCATGGGCTGGTATTCCAGATTATTTTAAAGGT
CCAGGATAAGTTCCTTGATGTCATCAAGCCACCTAGGAGGGATAAGAACA
TGAGAACGACAGTGAAGTATTTAAAAACTCATCAAATATTGAGCAGCGGA
GGAGACTATTCTAGTTACTTAATGAAGGTGCAACGACCATTTGTTGGTGA
ATGTGGAGATGTACACAGATTTGTTTCTGCGGTCGGTGCTAGCATAGATG
TTTCTACCATGCTGCAGTCCATGTATTCAATAGAACAGTTGATATGTAAC
TTTGGGGTCGACATTGGTGTCGATCAATCTACGGAATACCTAATGAATTC
AGCAGTCAATGTTGTTGTACAATATGATATCGAGATGGATGGCATTATTT
GGGATTTGGTATCAAGGTTATGGGCTGCAAATATTTCCGCAGAGATATAT
GATAAAGATGAGCATGGGGAAGAATACGTTTTCAATCAGAAAATCCATTT
CTATGTCAGGTTTCCGAGTAGATATGAAGAAAAATGTTACTCAGACGCCA
GAATTTCAGTGAAAAGGAACGATTGGAAGGGATTGGGTCTGGAACAAATA
TCGAATGACAATGTTAAAAAACCGGCAAACGTTGTAAATTTGATTTTGAG
AGAAATGAAGAAATCCAAAGAAAACTTATTTAATCGATTTGAAAAAGAAC
CATATCAAAGGCAAACTAGCGACTCTCTGTCAGCGGACTCGTGCACGGAT
TTCGACAGTAAAAAGATAAATAACCTGAAAACCGAAACGAATCCAACCAG
ATATATATACAAGTTTATCACCGCTAAAGAAAGCAGCAAATTTGGCTCTC
AAGAATTTACTATTCCCTATTTTGTGGAATCCAATAAACAAATGCAGTTA
CTCAATCAGAAAACCCCGAATTTGGAACTCCCACAGATCCAAATGATTCG
GAAAGTGAATTGCCAGTTGCTAAATGTTAACAAATGGAATCAAGGCAAAT
TCCCCAATCCTCTCTATTGCTTGATTTGTGATATATCGCACTCTAAATGC
CTTAGCCTAGTGGAGGAGGTTTCCCTGAAATCGCTGAAGGAAACCAATCG
GCTGACGAAGCTATTCAATGAAATAGAGTCCTCAAGGAATTACCAGAACA
ATGTTGAAAAGCAATGGAAAGATGTTCTTGCTAGAATTTGGGAATCTCTC
CAGAATGCAGTTAAAGAAAAGCTGTTTCGCATTGTCATCTTTAGCCAGAT
CGATGAGAATTTTGTGTTTTTGGAAATAAAATAAATCGCTTTTGTTTT

protein sequence of SMED30032249-orf-1

>SMED30032249-orf-1 ID=SMED30032249-orf-1|Name=SMED30032249-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1696bp
MSSYDVTILQSDEVKALRSIFGKEFKLIRPKNLKKKYEPPLVSVLINVDS
SNLNSALSLRISFDCPQNYPFDIPKISIYDAVNVPTNLVEQLLIILNEKS
NLLKGYSMIYNLVEEARCIIIKHFETDHKSVNSILTINKVLNEDEIMQTE
TKQSEFLKNLLRQRRDSARNEKKNEKKFEKCDHSYKTDELEFEHCSDRSS
GCLVYKGICDGQHRIKTINPNMQIKLNTFNGFNRQKGTNVKIYQIRFSDL
KRKQFEDLKSNWLSKLEEDFTLLYKLNHPHLITYYGFRYQAHEKVIRKTK
HFDLVIQYVTSNDEGLKLWKVCEMDTKPGSGDNALKTRLSISKIINYLKS
ILEAVIYLHDKNITFIKINMDSILIDKDLQLKICNFCIGKRFEELKGRGH
SNERTGASTIKKGSKRYDLMKLGVLAKELFFFGIPETEREETDMPSDLID
FITSCNSNIEELKLHPFLTCENLSKNSRQNSQTVDSAQDLTPWHLFEKSS
RIPKEFELICKLGEGAFGDVVKVRNIYDNQYYAIKRVRLNAIKKKAARTM
KEVQLLSSLNHENIVRYFASWSEEMQKSSSETNNLQPNIDSSSIRNNTSS
AISVIKPETTDSLLLDFQDKKNSIMFSANVNFSQIEHCSRHFDLESFSDS
DSDSDSDSSVELKDQSPRYKQPSVEISFGYSDNDDNPLDNHHKKNTPDEL
SKQVKSMRLKSGYINDSQNNPFHGNKTKIEKYLMIQMELCEGQYNTLQKY
IDDQKLYMNEKLIWRFIREIVSGLEHIHSKNMIHRDLKPHNIFLDSQKRV
KIGDLGLAMNFNNKVTESANQEYVKDNGLSNDVENGPVGTIMYICPEMLN
QSSYTNKADMYSLGIIVFEMCHKPFGTGSERYHILGNLRRPEIIFPDEKH
FKIKSSFVELIKSLLNHDQLLRPSASELLQSNIIPPPEGKELELRKFLID
VTKNSSSNLYKLMLKELFSKLYSEICRYELSLLYQSPFNHFISNTKSLVF
RNLRNPLSSQIGEGDFFFFTLKRFVTRYIESIFVSHGAVEFLAPLLSPKF
INTESGFFSLLDAEGVVVNLPNNLRMNFANFLVDNRISCCKRYAIGPIYQ
QQAIRRDHPRERIEAAFDIVTDYDSPNKTAEILYLLHQIFKKSFNSEHAT
LTLEINNTNLFYALMNHYSIRASLHDMVSKDVKNLDSDHFRSLTNLTDKQ
KANIKSILLFNGSRLEDLEKHFKIFQNRIHEGYDVINSAFKELKSVEEKL
KHFTEDLCFCVNFRPSLVPLETIYHGLVFQIILKVQDKFLDVIKPPRRDK
NMRTTVKYLKTHQILSSGGDYSSYLMKVQRPFVGECGDVHRFVSAVGASI
DVSTMLQSMYSIEQLICNFGVDIGVDQSTEYLMNSAVNVVVQYDIEMDGI
IWDLVSRLWAANISAEIYDKDEHGEEYVFNQKIHFYVRFPSRYEEKCYSD
ARISVKRNDWKGLGLEQISNDNVKKPANVVNLILREMKKSKENLFNRFEK
EPYQRQTSDSLSADSCTDFDSKKINNLKTETNPTRYIYKFITAKESSKFG
SQEFTIPYFVESNKQMQLLNQKTPNLELPQIQMIRKVNCQLLNVNKWNQG
KFPNPLYCLICDISHSKCLSLVEEVSLKSLKETNRLTKLFNEIESSRNYQ
NNVEKQWKDVLARIWESLQNAVKEKLFRIVIFSQIDENFVFLEIK*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **biological process**
Term	Definition
GO:0000077	DNA damage checkpoint
GO:0010998	regulation of translational initiation by eIF2 alpha phosphorylation
GO:0006468	protein phosphorylation
GO:0006413	translational initiation
GO:0016310	phosphorylation

Vocabulary: **molecular function**
Term	Definition
GO:0000166	nucleotide binding
GO:0004694	eukaryotic translation initiation factor 2alpha kinase activity
GO:0005524	ATP binding
GO:0004672	protein kinase activity
GO:0005515	protein binding
GO:0003743	translation initiation factor activity
GO:0016301	kinase activity

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000101	muscle cell
PLANA:0002109	X1 cell
PLANA:0003001	lateral region of the whole animal

Vocabulary: **INTERPRO**
Term	Definition
IPR016255	Gcn2
IPR041715	HisRS-like_core
IPR000719	Prot_kinase_dom
IPR006575	RWD-domain
IPR016135	UBQ-conjugating_enzyme/RWD
IPR017441	Protein_kinase_ATP_BS
IPR008271	Ser/Thr_kinase_AS
IPR011009	Kinase-like_dom_sf
IPR011528	NERD

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 1240..1260
None	No IPR available	GENE3D	G3DSA:3.30.200.20		coord: 496..608 e-value: 6.3E-27 score: 95.8
None	No IPR available	GENE3D	G3DSA:1.10.510.10		coord: 711..939 e-value: 2.1E-48 score: 166.5
None	No IPR available	GENE3D	G3DSA:3.30.930.10		coord: 1022..1368 e-value: 8.3E-39 score: 135.5
None	No IPR available	GENE3D	G3DSA:1.10.510.10		coord: 227..495 e-value: 2.6E-14 score: 54.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 674..688
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 649..706
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 689..706
None	No IPR available	PANTHER	PTHR11042	EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED	coord: 91..1256
None	No IPR available	PANTHER	PTHR11042:SF136	EIF-2-ALPHA KINASE GCN2	coord: 91..1256
None	No IPR available	SUPERFAMILY	SSF55681	Class II aaRS and biotin synthetases	coord: 1023..1366
IPR006575	RWD domain	SMART	SM00591	RWD2001b	coord: 12..123 e-value: 1.5E-8 score: 44.4
IPR006575	RWD domain	PFAM	PF05773	RWD	coord: 10..116 e-value: 1.5E-13 score: 51.0
IPR006575	RWD domain	PROSITE	PS50908	RWD	coord: 12..123 score: 16.645
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 506..938 e-value: 5.8E-48 score: 175.3
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 507..574 e-value: 3.9E-12 score: 46.1 coord: 734..936 e-value: 4.5E-39 score: 134.4 coord: 264..395 e-value: 4.2E-10 score: 39.4
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 506..938 score: 39.764
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 218..497 score: 12.612
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	GENE3D	G3DSA:3.10.110.10		coord: 3..129 e-value: 5.7E-20 score: 73.3
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	SUPERFAMILY	SSF54495	UBC-like	coord: 9..117
IPR016255	eIF-2-alpha kinase Gcn2	PIRSF	PIRSF000660	STPK_GCN2	coord: 2..411 e-value: 1.0E-8 score: 30.3 coord: 439..1552 e-value: 2.7E-102 score: 340.5
IPR041715	Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domain	PFAM	PF13393	tRNA-synt_His	coord: 1096..1288 e-value: 2.0E-7 score: 30.6
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 786..798
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 512..544
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	SSF56112	Protein kinase-like (PK-like)	coord: 263..475
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	SSF56112	Protein kinase-like (PK-like)	coord: 480..936