Arachidonate 5-lipoxygenase
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30032236 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 9
Homology
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Human
Match: ALOX5 (arachidonate 5-lipoxygenase [Source:HGNC Symbol;Acc:HGNC:435]) HSP 1 Score: 171.014 bits (432), Expect = 8.419e-44 Identity = 154/673 (22.88%), Postives = 297/673 (44.13%), Query Frame = 2
Query: 323 KLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDP---FSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAINQRRLTD-------LYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLNC-KPD----RIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDP-MQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQ-TSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQS--VLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMDPSILPN 2278
++E ++W ++ DWYL+ + ++ HG +Y FP ++WI G + D +D + R +E+ +Q+ + + + GFP+ ++ + + ++D + +N + + ++ QS W+ + ++ + + T + N D+ FG Q L GCNP++++ CTE+P+ VT E ++ LE S++ ++ ++IVDF+++DG++ K D + + AP+ L + ++ P+AIQL Q + N +FLPSD WLLAK+W +D H + L+ ++ ++ + M R HPI++LL H + ++ K +E + + G + G Q+V+RA K+ ++ + + +++R + + + Y YRDD LL+ A F ++V ++Y D +++E DP +QD + +G KS R++ L +F+ Q +VNF YD + +P+ P P + ++Q+V LPD+ + L +V ++ ++ LG + ++ + + + FRK LEAI I NK + + YY + P +PN
Sbjct: 69 RIEKRKYWLND------DWYLKYITLKTP-HG-----DYIEFPCYRWITGDVEVVLRDGRAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHK-DLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNTISERVMNHWQEDLMFGYQFLNGCNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQEVQQGNIFIVDFELLDGIDANKTDPCTLQFLAAPICLLYKNLANKIVPIAIQLNQIPGDENPIFLPSDAKYDWLLAKIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPIFKLLVAHVRFTIAINTKAREQLICECGLFDKANATGGGGHVQMVQRAMKDLTY-ASLCFPEAIKARGMESKEDIPYYFYRDDGLLVWEAIRTFTAEVVDIYY---EGDQVVEE----------DPELQDFVNDVYVYGMRGRKSSGFPKSVKSREQLSEYLTVVIFTASAQHAAVNFGQ-------------------------------YDWCSW-----------IPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMARFRKNLEAIVSVIAERNKKKQL--PYYYLSPDRIPN 670
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Human
Match: ALOX5 (arachidonate 5-lipoxygenase [Source:HGNC Symbol;Acc:HGNC:435]) HSP 1 Score: 171.014 bits (432), Expect = 8.419e-44 Identity = 154/673 (22.88%), Postives = 297/673 (44.13%), Query Frame = 2
Query: 323 KLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDP---FSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAINQRRLTD-------LYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLNC-KPD----RIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDP-MQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQ-TSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQS--VLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMDPSILPN 2278
++E ++W ++ DWYL+ + ++ HG +Y FP ++WI G + D +D + R +E+ +Q+ + + + GFP+ ++ + + ++D + +N + + ++ QS W+ + ++ + + T + N D+ FG Q L GCNP++++ CTE+P+ VT E ++ LE S++ ++ ++IVDF+++DG++ K D + + AP+ L + ++ P+AIQL Q + N +FLPSD WLLAK+W +D H + L+ ++ ++ + M R HPI++LL H + ++ K +E + + G + G Q+V+RA K+ ++ + + +++R + + + Y YRDD LL+ A F ++V ++Y D +++E DP +QD + +G KS R++ L +F+ Q +VNF YD + +P+ P P + ++Q+V LPD+ + L +V ++ ++ LG + ++ + + + FRK LEAI I NK + + YY + P +PN
Sbjct: 69 RIEKRKYWLND------DWYLKYITLKTP-HG-----DYIEFPCYRWITGDVEVVLRDGRAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHK-DLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNTISERVMNHWQEDLMFGYQFLNGCNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQEVQQGNIFIVDFELLDGIDANKTDPCTLQFLAAPICLLYKNLANKIVPIAIQLNQIPGDENPIFLPSDAKYDWLLAKIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPIFKLLVAHVRFTIAINTKAREQLICECGLFDKANATGGGGHVQMVQRAMKDLTY-ASLCFPEAIKARGMESKEDIPYYFYRDDGLLVWEAIRTFTAEVVDIYY---EGDQVVEE----------DPELQDFVNDVYVYGMRGRKSSGFPKSVKSREQLSEYLTVVIFTASAQHAAVNFGQ-------------------------------YDWCSW-----------IPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMARFRKNLEAIVSVIAERNKKKQL--PYYYLSPDRIPN 670
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Human
Match: ALOX5 (arachidonate 5-lipoxygenase [Source:HGNC Symbol;Acc:HGNC:435]) HSP 1 Score: 161.384 bits (407), Expect = 8.156e-41 Identity = 125/515 (24.27%), Postives = 244/515 (47.38%), Query Frame = 2
Query: 323 KLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDP---FSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAINQRRLTD-------LYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLNC-KPD----RIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDP-MQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQ-TSVNF 1810
++E ++W ++ DWYL+ + ++ HG +Y FP ++WI G + D +D + R +E+ +Q+ + + + GFP+ ++ + + ++D + +N + + ++ QS W+ + ++ + + T + N D+ FG Q L GCNP++++ CTE+P+ VT E ++ LE S++ ++ ++IVDF+++DG++ K D + + AP+ L + ++ P+AIQL Q + N +FLPSD WLLAK+W +D H + L+ ++ ++ + M R HPI++LL H + ++ K +E + + G + G Q+V+RA K+ ++ + + +++R + + + Y YRDD LL+ A F ++V ++Y D +++E DP +QD + +G KS R++ L +F+ Q +VNF
Sbjct: 69 RIEKRKYWLND------DWYLKYITLK-TPHG-----DYIEFPCYRWITGDVEVVLRDGRAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHK-DLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNTISERVMNHWQEDLMFGYQFLNGCNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQEVQQGNIFIVDFELLDGIDANKTDPCTLQFLAAPICLLYKNLANKIVPIAIQLNQIPGDENPIFLPSDAKYDWLLAKIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPIFKLLVAHVRFTIAINTKAREQLICECGLFDKANATGGGGHVQMVQRAMKDLTY-ASLCFPEAIKARGMESKEDIPYYFYRDDGLLVWEAIRTFTAEVVDIYYEG---DQVVEE----------DPELQDFVNDVYVYGMRGRKSSGFPKSVKSREQLSEYLTVVIFTASAQHAAVNF 556
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Human
Match: ALOX5 (arachidonate 5-lipoxygenase [Source:HGNC Symbol;Acc:HGNC:435]) HSP 1 Score: 161.384 bits (407), Expect = 8.156e-41 Identity = 125/515 (24.27%), Postives = 244/515 (47.38%), Query Frame = 2
Query: 323 KLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDP---FSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAINQRRLTD-------LYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLNC-KPD----RIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDP-MQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQ-TSVNF 1810
++E ++W ++ DWYL+ + ++ HG +Y FP ++WI G + D +D + R +E+ +Q+ + + + GFP+ ++ + + ++D + +N + + ++ QS W+ + ++ + + T + N D+ FG Q L GCNP++++ CTE+P+ VT E ++ LE S++ ++ ++IVDF+++DG++ K D + + AP+ L + ++ P+AIQL Q + N +FLPSD WLLAK+W +D H + L+ ++ ++ + M R HPI++LL H + ++ K +E + + G + G Q+V+RA K+ ++ + + +++R + + + Y YRDD LL+ A F ++V ++Y D +++E DP +QD + +G KS R++ L +F+ Q +VNF
Sbjct: 69 RIEKRKYWLND------DWYLKYITLK-TPHG-----DYIEFPCYRWITGDVEVVLRDGRAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHK-DLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNTISERVMNHWQEDLMFGYQFLNGCNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQEVQQGNIFIVDFELLDGIDANKTDPCTLQFLAAPICLLYKNLANKIVPIAIQLNQIPGDENPIFLPSDAKYDWLLAKIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPIFKLLVAHVRFTIAINTKAREQLICECGLFDKANATGGGGHVQMVQRAMKDLTY-ASLCFPEAIKARGMESKEDIPYYFYRDDGLLVWEAIRTFTAEVVDIYYEG---DQVVEE----------DPELQDFVNDVYVYGMRGRKSSGFPKSVKSREQLSEYLTVVIFTASAQHAAVNF 556
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Human
Match: ALOX12B (arachidonate 12-lipoxygenase, 12R type [Source:HGNC Symbol;Acc:HGNC:430]) HSP 1 Score: 157.147 bits (396), Expect = 4.227e-39 Identity = 125/514 (24.32%), Postives = 221/514 (43.00%), Query Frame = 2
Query: 731 WSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHL-EGWSVKHVIKARRLYIVDFKIMDGLNC----KPDRIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLI-NQMILENIYVCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVT--QSVLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQY 2248
W L+++ ++ + D FG Q L G NP +++ CT IPD F VT + + P L EG ++ ++ +Y+ D++IM+G+ + AP+ L F E ++ P+AIQL+Q+ +FLPSD WLLAK W A+ H + L+ +I E + + RN HP+Y+LL PH + ++ + + ++ G A+ + L +G ++ RA ++D + + D R V DL Y YRDD+L + NA +V +++ +YP+ + D ELQ+W +E+ + L R+ S F + +L+ +T + I C + ++ ++ +P+ P P+Q + L+ + LPD +T L+ + +R + LG F + + + +E FR++L I DI + NK I + Y
Sbjct: 233 WKRLKDIRKIFPGKKSVVSEYVAEHWAEDTFFGYQYLNGVNPGLIRRCTRIPDKFPVTDDMVAPFLGEGTCLQAELEKGNIYLADYRIMEGIPTVELSGRKQHHCAPLCLLHFGPEGKMMPIAIQLSQTPGPDCPIFLPSDSEWDWLLAKTWVRYAEFYSHEAIAHLLETHLIAEAFCLALLRNLPMCHPLYKLLIPHTRYTVQINSIGRAVLLNEGGLSAKGMSLGVEGFAGVMVRALSELTYD-SLYLPNDFVERGVQDLPG---YYYRDDSLAVWNALEKYVTEIITYYYPSD-AAVEGDPELQSWVQEIFK-----------------------ECLLGRE-----------------SSGFPRCLRTVPELIRYVT----IVIYTCSA--------KHAAVNTGQMEFTAWMPNFPASMRNPPIQTKGLTTLETFMDTLPDVKTTCITLLVLWTLSREPDDRRPLGHFPDIHFVEEAPRRSIEAFRQRLNQISHDIRQRNKCLPIPYYY 689
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Zebrafish
Match: alox5b.2 (arachidonate 5-lipoxygenase b, tandem duplicate 2 [Source:ZFIN;Acc:ZDB-GENE-060623-19]) HSP 1 Score: 175.252 bits (443), Expect = 2.560e-45 Identity = 150/660 (22.73%), Postives = 291/660 (44.09%), Query Frame = 2
Query: 362 MMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDPFS---ETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXX-----XKFSKSEKWDIDNAKLDAINQRRLTDLYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLNCKPDRIMPA-------PMALFFFTEEKQLKPLAIQLTQSSNEG--NLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARV--TQSVLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMDPSILPN 2278
+++ DWY + + + F FP ++WI + + + ++ S E R E+ +++E F + SGF + ++ +F ++ D A L L W + ++ TE + + D FG Q L GCNP+++K CT++PD F VT E ++ L+ G +++ +KA +YI D++I++ + +P+ P+ P LF+ + + P+AIQL++ + G N VFLP+D WLLAKMW +D H +V L+ ++ ++ + M R HP+Y+LL PH + ++ +E + ++ G + GI+Q++++ + ++ + + ++ R V + + L NY YRDD +++ A FV +VK +Y + + + DEE+Q + +++ G +N N NG F N + E LV LT I+ + + + +D Y + +P+ P+ P + ++ ++ +LPD + L ++ + ++ + LG + Y + A++ +++FRK+L + I R N+ ++ + Y C P +PN
Sbjct: 76 LINDDWYCRSISVTAPTG------DCFEFPCYRWIADQKELVLPEGRGRLPQDEKLSILKECRHLELENRREKFRWNEWKSGFLMSIDANKSSELPLDVQFDTAKAVDFSLNAFKAAENLGLNKLESLFQSWKDFADFEKVFITIKNTESEYVMQNWTKDAVFGYQFLNGCNPVMIKKCTKLPDKFAVTQEMVENSLDRGTTLQEELKAGNIYIADYEILE--DVQPNDTDPSTQQYLATPFCLFYKNGQNEFIPIAIQLSRQTTPGEMNTVFLPTDDEYDWLLAKMWVKSSDYNVHQLVTHLLRTHLISEVFTIAMFRQLSAVHPVYKLLIPHVRFTIAINTAARETLINEGGVFGKVDSTGGIGIEQVIRKGMQTLTYK-SLCFPEAMKIRNVDNKEDLPNYYYRDDGMMVWEAVKIFVSDVVKFYY-SSDETVQEDEEIQAFVQDVCS------------YGMKNCPN----------------NGE-----------FPNALKTREQLVEYLT-IVIFTASAQHAAINFGQFDWYAW-----------IPNTPSTMRKPPPIEKGKVDMKYIMESLPDHGRSSLHLGTIWILSQFQDNELFLGVYPDKYFTEEPAMEAIKNFRKKLVDVTKTIRRRNETLELPYWYLC--PDRIPN 672
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Zebrafish
Match: alox5a (arachidonate 5-lipoxygenase a [Source:ZFIN;Acc:ZDB-GENE-090311-47]) HSP 1 Score: 165.622 bits (418), Expect = 3.958e-42 Identity = 154/667 (23.09%), Postives = 295/667 (44.23%), Query Frame = 2
Query: 323 KLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDPFS---ETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAIN-QRRLTDLYGDQ------SFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLNCKPD-----RIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRREL-SDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQS--VLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMDP 2263
K+E ++W M DWY + + ++ Y +Y FP +W+ + I D D + R +E+ +Q+ + + GFP+ ++ + + + ++D + +N + + +LY +Q S W + ++ + T + D FG Q L GCNP+V+K CTEIPD F VT ++ LE G +++ +K ++I D++IMDG+ + + APM L + + ++ P+AIQL Q E N +FLPSD WLLAK+W +D H V L+ ++ ++ + M R HP+++LL PH + ++ K +E + + G + G +LV+R+ K +++ + +++++R + + L Y YRDD + +FV +V ++Y + + + DEE+Q + +++ S MQD LD+ F ++ E+LV LT ++ + + + YD + N S PT R P++ + + +L +V +LPD+ + L +V ++ ++ LG + ++ + +++ F K+L + I+ N+ + + YY + P
Sbjct: 69 KIEKKKYW------MHDDWYCKYITVK------TPYGDYVEFPCFRWLVDDKEVILRDGRARLPQHDKTGVAKQHRRKELEQRQKTYRWKEWHPGFPMSIDCKAHK-ELPRDIQFDSEKGVDFVLNYSKAIENLYVNQFMHMFQSSWGDFADFERIFVRIKNTISEYVMEHWKEDFMFGYQYLNGCNPVVIKKCTEIPDKFPVTHNIVEDSLERGLTLEEELKEGNIFIADYEIMDGVTANSTDPCTLQYLAAPMCLLYKNSQNKIMPIAIQLHQKPGEDNPIFLPSDDEYDWLLAKIWVRSSDFHVHQTVTHLLRTHLISEVFGIAMFRQLPAVHPVFKLLLPHIRFTIAINTKAREQLICECGLFDKANGTGGGGHIELVQRSMKVFTYK-SLCFPENIKARGMDSQEELPYYFYRDDGCAVWETVKSFVSDVVDIYY-DKDETVQEDEEIQAFVKDVCSFGMQD----------------------LDQ-------------------CEFPKSLQTKEELVEYLTVVI-FTASAQHAAVNFGQYDWCSWIPN----------SPPTMRKPPPMK-KGEVDLQYIVESLPDRGRSCWHLGAVWALSQFQENELFLGMYPDEHFIEKPVKEVMVKFGKKLAEVSKTIKNRNEGKKL--PYYYLSP 665
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Zebrafish
Match: alox5a (arachidonate 5-lipoxygenase a [Source:ZFIN;Acc:ZDB-GENE-090311-47]) HSP 1 Score: 165.236 bits (417), Expect = 5.255e-42 Identity = 154/667 (23.09%), Postives = 294/667 (44.08%), Query Frame = 2
Query: 323 KLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDPFS---ETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAIN-QRRLTDLYGDQ------SFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLNCKPD-----RIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRREL-SDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQS--VLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMDP 2263
K+E ++W M DWY + + ++ Y +Y FP +W+ + I D D + R +E+ +Q+ + + GFP+ ++ + + + ++D + +N + + +LY +Q S W + ++ + T + D FG Q L GCNP+V+K CTEIPD F VT ++ LE G +++ +K ++I D++IMDG+ + + APM L + + ++ P+AIQL Q E N +FLPSD WLLAK+W +D H V L+ ++ ++ + M R HP+++LL PH + ++ K +E + + G + G +LV+R+ K +++ + +++++R + + L Y YRDD + +FV +V ++Y + + + DEE+Q + +++ S MQD LD F ++ E+LV LT ++ + + + YD + N S PT R P++ + + +L +V +LPD+ + L +V ++ ++ LG + ++ + +++ F K+L + I+ N+ + + YY + P
Sbjct: 69 KIEKKKYW------MHDDWYCKYITVK------TPYGDYVEFPCFRWLVDDKEVILRDGRARLPQHDKTGVAKQHRRKELEQRQKTYRWKEWHPGFPMSIDCKAHK-ELPRDIQFDSEKGVDFVLNYSKAIENLYVNQFMHMFQSSWGDFADFERIFVRIKNTISEYVMEHWKEDFMFGYQYLNGCNPVVIKKCTEIPDKFPVTHNIVEDSLERGLTLEEELKEGNIFIADYEIMDGVTANSTDPCTLQYLAAPMCLLYKNSQNKIMPIAIQLHQKPGEDNPIFLPSDDEYDWLLAKIWVRSSDFHVHQTVTHLLRTHLISEVFGIAMFRQLPAVHPVFKLLLPHIRFTIAINTKAREQLICECGLFDKANGTGGGGHIELVQRSMKVFTYK-SLCFPENIKARGMDSQEELPYYFYRDDGCAVWETVKSFVSDVVDIYY-DKDETVQEDEEIQAFVKDVCSFGMQD----------------------LDH-------------------CEFPKSLQTKEELVEYLTVVI-FTASAQHAAVNFGQYDWCSWIPN----------SPPTMRKPPPMK-KGEVDLQYIVESLPDRGRSCWHLGAVWALSQFQENELFLGMYPDEHFIEKPVKEVMVKFGKKLAEVSKTIKNRNEGKKL--PYYYLSP 665
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Zebrafish
Match: si:dkey-17e16.9 (si:dkey-17e16.9 [Source:ZFIN;Acc:ZDB-GENE-091118-123]) HSP 1 Score: 162.54 bits (410), Expect = 3.598e-41 Identity = 154/644 (23.91%), Postives = 294/644 (45.65%), Query Frame = 2
Query: 374 DWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDPF---SETRLEEIRHKQEIFSLYQRSSGFXXXXX-----XXXXXXKFSKSEKWDIDNA--KLDAINQRRLTDLYGDQSFWSSLENLHNLY--SQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLNCK----PDRIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIYVCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQSV--LGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQY 2248
DW+ V I+ + FP ++W+ + + K YE+ + E R E++ +E++ +G P + +VP +FS ++ DID+A + A+ + ++ L W +++++ + S+ I++ + L +D G Q L G NPL+L C+E+P NF V+ + L P LE G S+ +K +++ D+K + L + + + AP+ L + +E +L P+AIQL Q ++E + VFLPSD + WL AK++ AD H L ++ E + + RN HPI++LL PH L ++ ++ + DG ++Q + +G+ L+++A + ++ + + +D+ R+ L +L N+ YRDD L + FVR +V+ FY N D+ D ELQ+W ++ +G L+ Q+S + + E+L+ LT ++ ++ + LR +D YG P+ P + P + + + V+ ALPD +++ ++ + ++ + + LG++ DY Q K++ DF+ +L +E N + + ++Y
Sbjct: 82 DWFCSLVNIKTPEK------DVISFPCYRWMTKREVIELREAKATKIYEEQYPQLKEHRQNELKLNKEVYRWTVFKAGLPQQAVFQDPLSVPSVVRFSFTK--DIDSAFNRSTALREIKMKKLAKKADQWPAIKHMKRAFWPSKTTISKYVYL--HWKDDDFLGYQLLNGPNPLMLHRCSELPLNFAVSDDMLLPFLESGTSLNVEMKQGNIFLCDYKRLADLPTQVINGKQQFVTAPLCLLYKNQEGKLLPIAIQLQQEASEHSPVFLPSDSELDWLTAKLYVRNADCLEHQAALHL-RSLLAETFTLSLLRNLPTVHPIHKLLVPHIRYTLQMNSLVRHRLIGPDGVLSQCTSIGHEGMMALMRKALSDLTYS-SLCLPEDISGRQ---LDSLPNFFYRDDGLRVWAFINDFVRSVVEFFYQND-SDVQKDTELQDWINDI-------------------FIHGFLE---------------------QSSSGIPQSFSTVEELIKFLTMMI-FTVSAQHAALRAGQFD-YG----------SWFPNTPGSLKSPPPACKGSSDKNTVLQALPDINTSANLASVFWLLSKPSADLVPLGQYPEDYFCQTAVRKMMRDFQAELAFFSEYVEDRNADLLVPYKY 657
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Zebrafish
Match: alox5b.1 (arachidonate 5-lipoxygenase b, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-131219-3]) HSP 1 Score: 161.77 bits (408), Expect = 8.074e-41 Identity = 127/503 (25.25%), Postives = 233/503 (46.32%), Query Frame = 2
Query: 362 MMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDPFS---ETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXX-----XKFSKSEKWDIDNAKLDAINQRRLTDLYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGL-----NCKPDRIMPAPMALFFFTEEKQLKPLAIQLTQSSNEG--NLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRREL-SDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYAD-LNGSVFSGGTQ-TSVNF 1810
+++ DWY + + + F FP ++WI + + + ++ S E R E+ ++Q+ F + GFP+ ++ KF + D L I L G W + ++ T L D FG Q L GCNP+++K CTE+P F VT E ++ L+ G +++ ++A +YI D++I++ + + + + AP LF+ + + P+AIQL++ + G N VFLPSD WLLAKMW D H +V L+ ++ ++ + M R HP+Y+LL PH + ++ +E + + G + G++Q++++A + ++ + + +++R V + + L NY YRDD +L+ A +FV +VK++Y + + + DEE+Q + +++ S M++ KNG S L + + L +F+ Q +VNF
Sbjct: 76 LINDDWYCRSISVTAPAG------DCFEFPCYRWIADQKELVLPEGRGRLPQDEKLSIVKECRHLELENRQKHFRWKEWKPGFPMSIDVNGSSELPLDVKFDTEKVVDFKLHFLKGIGNVGLNKCKGYYQSWKDFADFEKVFITIENTVSEHLMQRWKEDAVFGYQFLNGCNPVMIKKCTELPGKFVVTQEMVRNSLDKGTTLQDELQAGNIYIADYEILEDVQPNDTDPSTQQYLAAPFCLFYKNGQNKFIPIAIQLSREATPGQMNTVFLPSDNHYDWLLAKMWVKSCDFNVHQLVTHLLRTHLISEVFAIAMFRQLSAVHPVYKLLIPHVRFTIAINTAAREKLINDTGVFNEANSSGGIGLQQVIQKAMQTLTYK-SLCFPEAMKTRGVDNQEDLPNYYYRDDGMLVWEAVKSFVSDVVKIYY-SSDETVQGDEEIQAFVQDVCSSGMKNC------------PKNGEFPSALKTQEQLVEYLTVVIFTASAQHAAVNF 558
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Xenopus
Match: ALOX5 (arachidonate 5-lipoxygenase [Source:NCBI gene;Acc:100036609]) HSP 1 Score: 161.384 bits (407), Expect = 1.362e-40 Identity = 152/676 (22.49%), Postives = 296/676 (43.79%), Query Frame = 2
Query: 323 KLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDP---FSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAINQRRLTD-------LYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLNC-KPD----RIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSG-GTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQS--VLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMDPSILPNYPGV 2290
K+E ++W + DWY + ++ +Y FP ++WI + + + +D + R +E++ +Q+++ + GFP+ ++ ++D + +N + + ++ QS W+ + ++ + + T + D FG Q L GCNP+++K C +IP NF VT E ++ LE +++ I+ ++IV+++++DG++ K D + + AP+ L + E ++ P+AIQL Q N +FLPSD WLLAK+W +D H V L+ +L ++ + M R HPI++LL H + ++ K +E + + G + G +L+ RA K ++++ + + + ++ R + + + Y YRDDAL + A FV+ +V ++Y + + + DEELQ + +++ +NT +G K+ +DK L +F+ G +VNF YD + +P+ P P + + ++ ++ +LPD+ + L +V ++ + LG + ++ + + + FRK LE I I+ NKN+ + YY + P +PN V
Sbjct: 69 KIEKHKYWYKD------DWYCRHLTVKTPSG------DYLEFPCYRWITNDKEIVLREGTAKLSRDDVTQVLKQHRRKELQERQKLYRWKEWYPGFPLSIDAHSHS-DLPHDIQFDSEKGVDFVLNYTKAMENLFVNSFMHMFQSSWNDFADFEKIFVRISNTISEYVMQHWQEDFMFGYQFLNGCNPVMIKKCKQIPANFPVTNEMVECSLERNLTLEQEIEKGNIFIVNYELLDGISANKTDPCTMQYIAAPICLLYKNLENKIVPIAIQLNQEPGPENPIFLPSDTHYDWLLAKIWVRSSDFHVHQTVSHLLRSHLLSEVFAIAMYRQLPAVHPIFKLLIAHVRFTIAINTKAREQLICECGLFDKANATGGGGHVELLLRAMKYFTYE-SLCLPEAIQKRGMESKEDIPYYYYRDDALKVWEATLNFVKDVVNIYYASD-ETVCEDEELQAFVKDV-----------FVYGLRDNTDSGFPKAIKTKDKLTEYLTLVIFTASGQHAAVNFGQ-------------------------------YDWCSW-----------IPNSPPTMRQPPPKEKGVVTIEFIIESLPDRGRSCWHLGAVWALSQFQDNELFLGYYPDEHFVEKSVKEAMVKFRKNLEEIASFIQDRNKNKKL--PYYYLSPDRIPNSVAV 674
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Xenopus
Match: ALOX5 (arachidonate 5-lipoxygenase [Source:NCBI gene;Acc:100036609]) HSP 1 Score: 159.458 bits (402), Expect = 4.620e-40 Identity = 132/533 (24.77%), Postives = 242/533 (45.40%), Query Frame = 2
Query: 722 QSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLNC-KPD----RIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSG-GTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARV--TQSVLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMDPSILPNYPGV 2290
QS W+ + ++ + + T + D FG Q L GCNP+++K C +IP NF VT E ++ LE +++ I+ ++IV+++++DG++ K D + + AP+ L + E ++ P+AIQL Q N +FLPSD WLLAK+W +D H V L+ +L ++ + M R HPI++LL H + ++ K +E + + G + G +L+ RA K ++++ + + + ++ R + + + Y YRDDAL + A FV+ +V ++Y + + + DEELQ + +++ +NT +G K+ +DK L +F+ G +VNF YD + +P+ P P + + ++ ++ +LPD+ + L +V ++ + LG + ++ + + + FRK LE I I+ NKN+ + YY + P +PN V
Sbjct: 179 QSSWNDFADFEKIFVRISNTISEYVMQHWQEDFMFGYQFLNGCNPVMIKKCKQIPANFPVTNEMVECSLERNLTLEQEIEKGNIFIVNYELLDGISANKTDPCTMQYIAAPICLLYKNLENKIVPIAIQLNQEPGPENPIFLPSDTHYDWLLAKIWVRSSDFHVHQTVSHLLRSHLLSEVFAIAMYRQLPAVHPIFKLLIAHVRFTIAINTKAREQLICECGLFDKANATGGGGHVELLLRAMKYFTYE-SLCLPEAIQKRGMESKEDIPYYYYRDDALKVWEATLNFVKDVVNIYYASD-ETVCEDEELQAFVKDV-----------FVYGLRDNTDSGFPKAIKTKDKLTEYLTLVIFTASGQHAAVNFGQ-------------------------------YDWCSW-----------IPNSPPTMRQPPPKEKGVVTIEFIIESLPDRGRSCWHLGAVWALSQFQDNELFLGYYPDEHFVEKSVKEAMVKFRKNLEEIASFIQDRNKNKKL--PYYYLSPDRIPNSVAV 654
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Xenopus
Match: ALOX5 (arachidonate 5-lipoxygenase [Source:NCBI gene;Acc:100036609]) HSP 1 Score: 156.762 bits (395), Expect = 1.493e-39 Identity = 128/502 (25.50%), Postives = 231/502 (46.02%), Query Frame = 2
Query: 815 DICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLNC-KPD----RIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSG-GTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARV--TQSVLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMDPSILPNYPGV 2290
D FG Q L GCNP+++K C +IP NF VT E ++ LE +++ I+ ++IV+++++DG++ K D + + AP+ L + E ++ P+AIQL Q N +FLPSD WLLAK+W +D H V L+ +L ++ + M R HPI++LL H + ++ K +E + + G + G +L+ RA K ++++ + + + ++ R + + + Y YRDDAL + A FV+ +V ++Y + + + DEELQ + +++ +NT +G K+ +DK L +F+ G +VNF YD + +P+ P P + + ++ ++ +LPD+ + L +V ++ + LG + ++ + + + FRK LE I I+ NKN+ + YY + P +PN V
Sbjct: 144 DFMFGYQFLNGCNPVMIKKCKQIPANFPVTNEMVECSLERNLTLEQEIEKGNIFIVNYELLDGISANKTDPCTMQYIAAPICLLYKNLENKIVPIAIQLNQEPGPENPIFLPSDTHYDWLLAKIWVRSSDFHVHQTVSHLLRSHLLSEVFAIAMYRQLPAVHPIFKLLIAHVRFTIAINTKAREQLICECGLFDKANATGGGGHVELLLRAMKYFTYE-SLCLPEAIQKRGMESKEDIPYYYYRDDALKVWEATLNFVKDVVNIYYASD-ETVCEDEELQAFVKDV-----------FVYGLRDNTDSGFPKAIKTKDKLTEYLTLVIFTASGQHAAVNFGQ-------------------------------YDWCSW-----------IPNSPPTMRQPPPKEKGVVTIEFIIESLPDRGRSCWHLGAVWALSQFQDNELFLGYYPDEHFVEKSVKEAMVKFRKNLEEIASFIQDRNKNKKL--PYYYLSPDRIPNSVAV 588
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Xenopus
Match: ALOX5 (arachidonate 5-lipoxygenase [Source:NCBI gene;Acc:100036609]) HSP 1 Score: 154.836 bits (390), Expect = 8.231e-39 Identity = 129/532 (24.25%), Postives = 247/532 (46.43%), Query Frame = 2
Query: 323 KLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDP---FSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAINQRRLTD-------LYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLNC-KPD----RIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSG-GTQTSVNFKNTINNPEDLVSLLTGIL 1864
K+E ++W + DWY + ++ +Y FP ++WI + + + +D + R +E++ +Q+++ + GFP+ ++ ++D + +N + + ++ QS W+ + ++ + + T + D FG Q L GCNP+++K C +IP NF VT E ++ LE +++ I+ ++IV+++++DG++ K D + + AP+ L + E ++ P+AIQL Q N +FLPSD WLLAK+W +D H V L+ +L ++ + M R HPI++LL H + ++ K +E + + G + G +L+ RA K ++++ + + + ++ R + + + Y YRDDAL + A FV+ +V ++Y + + + DEELQ + +++ +NT +G K+ +DK L +F+ G +VNF LL+GIL
Sbjct: 69 KIEKHKYWYKD------DWYCRHLTVKTPSG------DYLEFPCYRWITNDKEIVLREGTAKLSRDDVTQVLKQHRRKELQERQKLYRWKEWYPGFPLSIDAHSHS-DLPHDIQFDSEKGVDFVLNYTKAMENLFVNSFMHMFQSSWNDFADFEKIFVRISNTISEYVMQHWQEDFMFGYQFLNGCNPVMIKKCKQIPANFPVTNEMVECSLERNLTLEQEIEKGNIFIVNYELLDGISANKTDPCTMQYIAAPICLLYKNLENKIVPIAIQLNQEPGPENPIFLPSDTHYDWLLAKIWVRSSDFHVHQTVSHLLRSHLLSEVFAIAMYRQLPAVHPIFKLLIAHVRFTIAINTKAREQLICECGLFDKANATGGGGHVELLLRAMKYFTYE-SLCLPEAIQKRGMESKEDIPYYYYRDDALKVWEATLNFVKDVVNIYYASD-ETVCEDEELQAFVKDV-----------FVYGLRDNTDSGFPKAIKTKDKLTEYLTLVIFTASGQHAAVNFGQV----NQYFFLLSGIL 570
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Xenopus
Match: ALOX5 (arachidonate 5-lipoxygenase [Source:NCBI gene;Acc:100036609]) HSP 1 Score: 151.369 bits (381), Expect = 1.596e-37 Identity = 124/514 (24.12%), Postives = 241/514 (46.89%), Query Frame = 2
Query: 323 KLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDP---FSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAINQRRLTD-------LYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLNC-KPD----RIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSG-GTQTSVNF 1810
K+E ++W + DWY + ++ +Y FP ++WI + + + +D + R +E++ +Q+++ + GFP+ ++ ++D + +N + + ++ QS W+ + ++ + + T + D FG Q L GCNP+++K C +IP NF VT E ++ LE +++ I+ ++IV+++++DG++ K D + + AP+ L + E ++ P+AIQL Q N +FLPSD WLLAK+W +D H V L+ +L ++ + M R HPI++LL H + ++ K +E + + G + G +L+ RA K ++++ + + + ++ R + + + Y YRDDAL + A FV+ +V ++Y + + + DEELQ + +++ +NT +G K+ +DK L +F+ G +VNF
Sbjct: 69 KIEKHKYWYKD------DWYCRHLTVKTPSG------DYLEFPCYRWITNDKEIVLREGTAKLSRDDVTQVLKQHRRKELQERQKLYRWKEWYPGFPLSIDAHSHS-DLPHDIQFDSEKGVDFVLNYTKAMENLFVNSFMHMFQSSWNDFADFEKIFVRISNTISEYVMQHWQEDFMFGYQFLNGCNPVMIKKCKQIPANFPVTNEMVECSLERNLTLEQEIEGN-IFIVNYELLDGISANKTDPCTMQYIAAPICLLYKNLENKIVPIAIQLNQEPGPENPIFLPSDTHYDWLLAKIWVRSSDFHVHQTVSHLLRSHLLSEVFAIAMYRQLPAVHPIFKLLIAHVRFTIAINTKAREQLICECGLFDKANATGGGGHVELLLRAMKYFTYE-SLCLPEAIQKRGMESKEDIPYYYYRDDALKVWEATLNFVKDVVNIYYASD-ETVCEDEELQAFVKDV-----------FVYGLRDNTDSGFPKAIKTKDKLTEYLTLVIFTASGQHAAVNF 555
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Mouse
Match: Alox5 (arachidonate 5-lipoxygenase [Source:MGI Symbol;Acc:MGI:87999]) HSP 1 Score: 172.555 bits (436), Expect = 1.931e-44 Identity = 155/672 (23.07%), Postives = 300/672 (44.64%), Query Frame = 2
Query: 323 KLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDP---FSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAINQRRLTD-------LYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLNC-KPD----RIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQ-TSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQS--VLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMDPSILPN 2278
K+E ++W + DWYL+ + ++ HG +Y FP ++WI G + D +D + R +E+ +Q+ + + + GFP+ ++ + + ++D + +N + + ++ QS W + ++ + + T + N D+ FG Q L GCNP+++K CT +P VT E ++ LE S++ ++ ++IVD++++DG++ K D + + AP+ L + ++ P+AIQL Q+ E N +FLP+D WLLAK+W +D H + L+ ++ ++ + M R HP+++LL H + ++ K +E + + G + G Q+V+RA ++ ++ + + +++R + + + Y YRDD LL+ A +F ++V ++Y N D +++E+ +EL D ++D + +G KS R+K L +F+ Q +VNF YD + +P+ P P + ++Q+V LPD+ + L +V ++ ++ LG + ++ + + + FRK LEAI I NKN+ + YY + P +PN
Sbjct: 69 KIEKRKYWLHD------DWYLKYITLK-TPHG-----DYIEFPCYRWITGEGEIVLRDGRAKLARDDQIHILKQHRRKELEARQKQYRWMEWNPGFPLSIDAKCHK-DLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWHDFADFEKIFVKISNTISERVKNHWQEDLMFGYQFLNGCNPVLIKRCTALPPKLPVTTEMVECSLERQLSLEQEVQEGNIFIVDYELLDGIDANKTDPCTHQFLAAPICLLYKNLANKIVPIAIQLNQTPGESNPIFLPTDSKYDWLLAKIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPLFKLLVAHVRFTIAINTKAREQLICEYGLFDKANATGGGGHVQMVQRAVQDLTYS-SLCFPEAIKARGMDSTEDIPFYFYRDDGLLVWEAIQSFTMEVVSIYYEN---DQVVEED-----QELQDFVKDVYVYGMRG----KKASGFPKSIKSREKLSEYLTVVIFTASAQHAAVNFGQ-------------------------------YDWCSW-----------IPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMIRFRKNLEAIVSVIAERNKNKKL--PYYYLSPDRIPN 670
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Mouse
Match: Alox5 (arachidonate 5-lipoxygenase [Source:MGI Symbol;Acc:MGI:87999]) HSP 1 Score: 161.384 bits (407), Expect = 5.651e-41 Identity = 125/514 (24.32%), Postives = 246/514 (47.86%), Query Frame = 2
Query: 323 KLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDP---FSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAINQRRLTD-------LYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLNC-KPD----RIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQ-TSVNF 1810
K+E ++W + DWYL+ + ++ HG +Y FP ++WI G + D +D + R +E+ +Q+ + + + GFP+ ++ + + ++D + +N + + ++ QS W + ++ + + T + N D+ FG Q L GCNP+++K CT +P VT E ++ LE S++ ++ ++IVD++++DG++ K D + + AP+ L + ++ P+AIQL Q+ E N +FLP+D WLLAK+W +D H + L+ ++ ++ + M R HP+++LL H + ++ K +E + + G + G Q+V+RA ++ ++ + + +++R + + + Y YRDD LL+ A +F ++V ++Y N D +++E+ +EL D ++D + +G KS R+K L +F+ Q +VNF
Sbjct: 69 KIEKRKYWLHD------DWYLKYITLK-TPHG-----DYIEFPCYRWITGEGEIVLRDGRAKLARDDQIHILKQHRRKELEARQKQYRWMEWNPGFPLSIDAKCHK-DLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWHDFADFEKIFVKISNTISERVKNHWQEDLMFGYQFLNGCNPVLIKRCTALPPKLPVTTEMVECSLERQLSLEQEVQEGNIFIVDYELLDGIDANKTDPCTHQFLAAPICLLYKNLANKIVPIAIQLNQTPGESNPIFLPTDSKYDWLLAKIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPLFKLLVAHVRFTIAINTKAREQLICEYGLFDKANATGGGGHVQMVQRAVQDLTYS-SLCFPEAIKARGMDSTEDIPFYFYRDDGLLVWEAIQSFTMEVVSIYYEN---DQVVEED-----QELQDFVKDVYVYGMRG----KKASGFPKSIKSREKLSEYLTVVIFTASAQHAAVNF 556
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Mouse
Match: Alox12b (arachidonate 12-lipoxygenase, 12R type [Source:MGI Symbol;Acc:MGI:1274782]) HSP 1 Score: 151.754 bits (382), Expect = 1.724e-37 Identity = 130/526 (24.71%), Postives = 225/526 (42.78%), Query Frame = 2
Query: 698 RLTDLYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHL-EGWSVKHVIKARRLYIVDFKIMDG-----LNCKPDRIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLI-NQMILENIYVCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVT--QSVLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQY 2248
+L L + W L+++ N++ + D FG Q L G NP +++ CT+IPD F VT E + P L EG ++ ++ +Y+ D++I+DG LN + APM L F + + P+AIQL+Q+ +FLP+D WLLAK W A+ H V L+ + +I E + + RN HP+Y+LL PH + ++ + + +K G A+ + L +G Q++ R ++ + I D R V DL Y +RDD+L + A +V +++ +YPN + D ELQ W +E+ + L R+ S F + +L+ +T +++ C + + + L+ +P+ P+ P+Q + L + LPD +T VL+ + R + LG F + + + +E FR+ L I +I + NK + + Y
Sbjct: 222 KLRGLVDRKRSWKRLKDIKNIFPATKSVVSEYVAEHWTEDSFFGYQYLNGINPGLIRRCTQIPDKFPVTDEMVAPFLGEGTCLQAELERGNIYLADYRILDGIPTVELNGQQQHHC-APMCLLHFGPDGNMMPIAIQLSQTPGPDCPIFLPNDSEWDWLLAKTWVRYAEFYSHEAVAHLLESHLIGEAFCLALLRNLPMCHPLYKLLIPHTRYNVQINSIGRALLLNKGGLSARAMSLGLEGFAQVMVRGLSELTYK-SLCIPNDFVERGVQDLPG---YYFRDDSLAVWYAMERYVTEIITYYYPNDAA-VEGDPELQCWVQEIFK-----------------------ECLLGRE-----------------SSGFPTCLRTIPELIEYVTMVMY----TCSA--------RHAAVNSGQLEYTSWMPNFPSSMRNPPMQTKGLTTLQTYMDTLPDVKTTCIVLLVLWTLCREPDDRRPLGHFPDIHFVEEGPRRSIEAFRQNLNQISHNIRQRNKCLTLPYYY 689
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Mouse
Match: Alox8 (arachidonate 8-lipoxygenase [Source:MGI Symbol;Acc:MGI:1098228]) HSP 1 Score: 151.369 bits (381), Expect = 1.806e-37 Identity = 113/418 (27.03%), Postives = 201/418 (48.09%), Query Frame = 2
Query: 446 FPVHQWIGGGRHYIFYDCETFKGYED---PFSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKL-----DAINQRRLTDLYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHL-EGWSVKHVIKARRLYIVDFKIMDG-----LNCKPDRIMPAPMALFFFTE-EKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRREL 1651
FP +QW+ G + + ++D + R +E+ +Q+++S G+P +++ ++ + + NAKL A + ++ L W SL + L++ + D F +Q L G NP++++ C +P+NF VT E + P L G S++ ++ L++VD I+ G LN KP + APM L + L P+AIQL Q+ N +FLPSD WLLAK W ++ H V L++ ++ ++ + R + HP+++LL PH L ++ +E + + + ++ L G L+KR + ++ V + +D+ +R V D+ Y YRDD + I A +FV ++V ++YP+ + D+ELQ W RE+
Sbjct: 105 FPCYQWLEGAGELVLREGAAKVSWQDHHPTLQDQRQKELESRQKMYSWKTYIEGWPRCLDHETVKDLDLNIKYSAMKNAKLFFKAHSAYTELKVKGLLDRTGLWRSLREMRRLFNFRKTPAAEYVFAHWQEDAFFASQFLNGINPVLIRRCHSLPNNFPVTDEMVAPVLGPGTSLQAELEKGSLFLVDHGILSGVHTNILNGKP-QFSAAPMTLLHQSSGSGPLLPIAIQLKQTPGPDNPIFLPSDDTWDWLLAKTWVRNSEFYIHEAVTHLLHAHLIPEVFALATLRQLPRCHPLFKLLIPHIRYTLHINTLARELLVAPGKLIDKSTGLGTGGFSDLIKRNMEQLNYSVLC-LPEDIRARGVEDIPG---YYYRDDGMQIWGAIKSFVSEIVSIYYPSD-TSVQDDQELQAWVREI 516
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Mouse
Match: Alox5 (arachidonate 5-lipoxygenase [Source:MGI Symbol;Acc:MGI:87999]) HSP 1 Score: 144.436 bits (363), Expect = 2.317e-35 Identity = 122/514 (23.74%), Postives = 233/514 (45.33%), Query Frame = 2
Query: 323 KLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDP---FSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAINQRRLTD-------LYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLNC-KPD----RIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQ-TSVNF 1810
K+E ++W + DWYL+ + ++ HG +Y FP ++WI G + D +D + R +E+ +Q+ + + + GFP+ ++ + + ++D + +N + + ++ QS W + ++ + + T + N D+ FG Q L GCNP+++K CT +P VT E ++ LE S++ ++ ++IVD++++DG++ K D + + AP+ L + ++ P+AIQL Q+ E N +FLP+D WLLAK+W +D H + L+ ++ ++ + M R HP+++LL H + ++ K +E + + G FD ++R + + + Y YRDD LL+ A +F ++V ++Y N D +++E+ +EL D ++D + +G KS R+K L +F+ Q +VNF
Sbjct: 69 KIEKRKYWLHD------DWYLKYITLK-TPHG-----DYIEFPCYRWITGEGEIVLRDGRAKLARDDQIHILKQHRRKELEARQKQYRWMEWNPGFPLSIDAKCHK-DLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWHDFADFEKIFVKISNTISERVKNHWQEDLMFGYQFLNGCNPVLIKRCTALPPKLPVTTEMVECSLERQLSLEQEVQEGNIFIVDYELLDGIDANKTDPCTHQFLAAPICLLYKNLANKIVPIAIQLNQTPGESNPIFLPTDSKYDWLLAKIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPLFKLLVAHVRFTIAINTKAREQLICEYGL------------------------FD---------KARGMDSTEDIPFYFYRDDGLLVWEAIQSFTMEVVSIYYEN---DQVVEED-----QELQDFVKDVYVYGMRG----KKASGFPKSIKSREKLSEYLTVVIFTASAQHAAVNF 524
BLAST of Arachidonate 5-lipoxygenase vs. UniProt/SwissProt
Match: sp|P51399|LOX5_MESAU (Arachidonate 5-lipoxygenase OS=Mesocricetus auratus OX=10036 GN=ALOX5 PE=2 SV=2) HSP 1 Score: 173.711 bits (439), Expect = 5.024e-44 Identity = 151/655 (23.05%), Postives = 289/655 (44.12%), Query Frame = 2
Query: 374 DWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDP---FSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAINQRRLTD-------LYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLNC-KPD----RIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMAD-ACHHMIVGRLINQMILENIYVCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQ-TSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQS--VLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMDPSILPN 2278
DWYL+ + ++ +Y FP ++WI G + D +D + R +E+ +Q+ + + + GFP+ ++ + + ++D + +N + + ++ QS W+ + ++ + + T + N D+ FG Q L GCNP+++K C E+P VT E ++ LE S++ ++ ++IVD++++DG++ K D + + AP+ L + ++ P+AIQL Q+ E N +FLPSD WLLAK+W +D H I L ++ E + M R HPI++LL H + ++ K +E + + G + G Q+V+RA ++ ++ + + +++R + + + Y YRDD LL+ A +F ++V ++Y + + ++ D+ELQ++ +++ +G KS R+K L +F+ Q +VNF YD + +P+ P P + ++Q+V LPD+ + L +V ++ ++ LG + ++ + + + FRK LEAI I NKN+ + YY + P +PN
Sbjct: 80 DWYLKYITLKTPT-------DYIEFPCYRWITGEGEIVLRDGRAKLARDDQIHILKQHRRKELEARQKQYRWMEWNPGFPLSIDAKCHK-DLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNTISERVKNHWQEDLMFGYQFLNGCNPVLIKRCRELPQKLPVTTEMVECSLERHLSLEQEVQEGNIFIVDYELLDGIDANKTDPCTHQFLAAPICLLYKNLANKIVPIAIQLNQAPGEKNPIFLPSDAKYDWLLAKIWVRSSDFHVHQTITHLLCTHLVSEVFGIAMYRQLPAVHPIFKLLVAHVRFTIAINTKAREQLICEYGLFDKANATGGGGHVQMVQRAVQDLTYS-SLCFPEAIKARGMDSTEDIPYYFYRDDGLLVWEAIQSFTSEVVSIYYEDDQV-VMEDQELQDFVKDV-----------YVYGMRGRKASGFPKSIKSREKLSEYLTVVIFTASAQHAAVNFGQ-------------------------------YDWCSW-----------IPNAPPTMRAPPATAKGVVTIEQIVATLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMTRFRKNLEAIVNVIAERNKNKKL--PYYYLSPDRIPN 669
BLAST of Arachidonate 5-lipoxygenase vs. UniProt/SwissProt
Match: sp|P48999|LOX5_MOUSE (Arachidonate 5-lipoxygenase OS=Mus musculus OX=10090 GN=Alox5 PE=1 SV=3) HSP 1 Score: 172.555 bits (436), Expect = 1.351e-43 Identity = 155/672 (23.07%), Postives = 300/672 (44.64%), Query Frame = 2
Query: 323 KLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDP---FSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAINQRRLTD-------LYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLNC-KPD----RIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQ-TSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQS--VLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMDPSILPN 2278
K+E ++W + DWYL+ + ++ HG +Y FP ++WI G + D +D + R +E+ +Q+ + + + GFP+ ++ + + ++D + +N + + ++ QS W + ++ + + T + N D+ FG Q L GCNP+++K CT +P VT E ++ LE S++ ++ ++IVD++++DG++ K D + + AP+ L + ++ P+AIQL Q+ E N +FLP+D WLLAK+W +D H + L+ ++ ++ + M R HP+++LL H + ++ K +E + + G + G Q+V+RA ++ ++ + + +++R + + + Y YRDD LL+ A +F ++V ++Y N D +++E+ +EL D ++D + +G KS R+K L +F+ Q +VNF YD + +P+ P P + ++Q+V LPD+ + L +V ++ ++ LG + ++ + + + FRK LEAI I NKN+ + YY + P +PN
Sbjct: 69 KIEKRKYWLHD------DWYLKYITLK-TPHG-----DYIEFPCYRWITGEGEIVLRDGRAKLARDDQIHILKQHRRKELEARQKQYRWMEWNPGFPLSIDAKCHK-DLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWHDFADFEKIFVKISNTISERVKNHWQEDLMFGYQFLNGCNPVLIKRCTALPPKLPVTTEMVECSLERQLSLEQEVQEGNIFIVDYELLDGIDANKTDPCTHQFLAAPICLLYKNLANKIVPIAIQLNQTPGESNPIFLPTDSKYDWLLAKIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPLFKLLVAHVRFTIAINTKAREQLICEYGLFDKANATGGGGHVQMVQRAVQDLTYS-SLCFPEAIKARGMDSTEDIPFYFYRDDGLLVWEAIQSFTMEVVSIYYEN---DQVVEED-----QELQDFVKDVYVYGMRG----KKASGFPKSIKSREKLSEYLTVVIFTASAQHAAVNFGQ-------------------------------YDWCSW-----------IPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMIRFRKNLEAIVSVIAERNKNKKL--PYYYLSPDRIPN 670
BLAST of Arachidonate 5-lipoxygenase vs. UniProt/SwissProt
Match: sp|P09917|LOX5_HUMAN (Arachidonate 5-lipoxygenase OS=Homo sapiens OX=9606 GN=ALOX5 PE=1 SV=2) HSP 1 Score: 171.014 bits (432), Expect = 4.043e-43 Identity = 154/673 (22.88%), Postives = 297/673 (44.13%), Query Frame = 2
Query: 323 KLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDP---FSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAINQRRLTD-------LYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLNC-KPD----RIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDP-MQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQ-TSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQS--VLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMDPSILPN 2278
++E ++W ++ DWYL+ + ++ HG +Y FP ++WI G + D +D + R +E+ +Q+ + + + GFP+ ++ + + ++D + +N + + ++ QS W+ + ++ + + T + N D+ FG Q L GCNP++++ CTE+P+ VT E ++ LE S++ ++ ++IVDF+++DG++ K D + + AP+ L + ++ P+AIQL Q + N +FLPSD WLLAK+W +D H + L+ ++ ++ + M R HPI++LL H + ++ K +E + + G + G Q+V+RA K+ ++ + + +++R + + + Y YRDD LL+ A F ++V ++Y D +++E DP +QD + +G KS R++ L +F+ Q +VNF YD + +P+ P P + ++Q+V LPD+ + L +V ++ ++ LG + ++ + + + FRK LEAI I NK + + YY + P +PN
Sbjct: 69 RIEKRKYWLND------DWYLKYITLKTP-HG-----DYIEFPCYRWITGDVEVVLRDGRAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHK-DLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNTISERVMNHWQEDLMFGYQFLNGCNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQEVQQGNIFIVDFELLDGIDANKTDPCTLQFLAAPICLLYKNLANKIVPIAIQLNQIPGDENPIFLPSDAKYDWLLAKIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPIFKLLVAHVRFTIAINTKAREQLICECGLFDKANATGGGGHVQMVQRAMKDLTY-ASLCFPEAIKARGMESKEDIPYYFYRDDGLLVWEAIRTFTAEVVDIYY---EGDQVVEE----------DPELQDFVNDVYVYGMRGRKSSGFPKSVKSREQLSEYLTVVIFTASAQHAAVNFGQ-------------------------------YDWCSW-----------IPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMARFRKNLEAIVSVIAERNKKKQL--PYYYLSPDRIPN 670
BLAST of Arachidonate 5-lipoxygenase vs. UniProt/SwissProt
Match: sp|P12527|LOX5_RAT (Arachidonate 5-lipoxygenase OS=Rattus norvegicus OX=10116 GN=Alox5 PE=1 SV=3) HSP 1 Score: 168.703 bits (426), Expect = 2.658e-42 Identity = 150/655 (22.90%), Postives = 293/655 (44.73%), Query Frame = 2
Query: 374 DWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDP---FSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAINQRRLTD-------LYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLNC-KPD----RIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQ-TSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQS--VLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMDPSILPN 2278
DWYL+ + ++ +Y FP ++WI G + D +D + R +E+ +Q+ + + + GFP+ ++ + + ++D + +N + + ++ QS W + ++ + + T + N D+ FG Q L GCNP+++K CTE+P VT E ++ LE S++ ++ ++IVD++++DG++ K D + + AP+ L + ++ P+AIQL Q+ E N +FLP+D WLLAK+W +D H + L+ ++ ++ + M R HP+++LL H + ++ K +E + + G + G Q+V+RA ++ ++ + + +++R + + + + Y YRDD LL+ A +F ++V ++Y + D +++E+ +EL D ++D + +G KS R+K L +F+ Q +VNF YD + +P+ P P + ++Q+V LPD+ + L +V ++ ++ LG + ++ + + + FRK LEAI I NKN+ + YY + P +PN
Sbjct: 80 DWYLKYITLKTPH-------DYIEFPCYRWITGEGEIVLRDGCAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHK-DLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWHDFADFEKIFVKISNTISERVKNHWQEDLMFGYQFLNGCNPVLIKRCTELPKKLPVTTEMVECSLERQLSLEQEVQEGNIFIVDYELLDGIDANKTDPCTHQFLAAPICLLYKNLANKIVPIAIQLNQTPGEKNPIFLPTDSKYDWLLAKIWVRSSDFHIHQTITHLLRTHLVSEVFGIAMYRQLPAVHPLFKLLVAHVRFTIAINTKAREQLNCEYGLFDKANATGGGGHVQMVQRAVQDLTYS-SLCFPEAIKARGMDNTEDIPYYFYRDDGLLVWEAIQSFTTEVVSIYYED---DQVVEED-----QELQDFVKDVYVYGMRG----RKASGFPKSIKSREKLSEYLTVVIFTASAQHAAVNFGQ-------------------------------YDWCSW-----------IPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMIRFRKNLEAIVSVIAERNKNKKL--PYYYLSPDRIPN 669
BLAST of Arachidonate 5-lipoxygenase vs. UniProt/SwissProt
Match: sp|O75342|LX12B_HUMAN (Arachidonate 12-lipoxygenase, 12R-type OS=Homo sapiens OX=9606 GN=ALOX12B PE=1 SV=1) HSP 1 Score: 157.147 bits (396), Expect = 2.030e-38 Identity = 125/514 (24.32%), Postives = 221/514 (43.00%), Query Frame = 2
Query: 731 WSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHL-EGWSVKHVIKARRLYIVDFKIMDGLNC----KPDRIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLI-NQMILENIYVCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVT--QSVLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQY 2248
W L+++ ++ + D FG Q L G NP +++ CT IPD F VT + + P L EG ++ ++ +Y+ D++IM+G+ + AP+ L F E ++ P+AIQL+Q+ +FLPSD WLLAK W A+ H + L+ +I E + + RN HP+Y+LL PH + ++ + + ++ G A+ + L +G ++ RA ++D + + D R V DL Y YRDD+L + NA +V +++ +YP+ + D ELQ+W +E+ + L R+ S F + +L+ +T + I C + ++ ++ +P+ P P+Q + L+ + LPD +T L+ + +R + LG F + + + +E FR++L I DI + NK I + Y
Sbjct: 233 WKRLKDIRKIFPGKKSVVSEYVAEHWAEDTFFGYQYLNGVNPGLIRRCTRIPDKFPVTDDMVAPFLGEGTCLQAELEKGNIYLADYRIMEGIPTVELSGRKQHHCAPLCLLHFGPEGKMMPIAIQLSQTPGPDCPIFLPSDSEWDWLLAKTWVRYAEFYSHEAIAHLLETHLIAEAFCLALLRNLPMCHPLYKLLIPHTRYTVQINSIGRAVLLNEGGLSAKGMSLGVEGFAGVMVRALSELTYD-SLYLPNDFVERGVQDLPG---YYYRDDSLAVWNALEKYVTEIITYYYPSD-AAVEGDPELQSWVQEIFK-----------------------ECLLGRE-----------------SSGFPRCLRTVPELIRYVT----IVIYTCSA--------KHAAVNTGQMEFTAWMPNFPASMRNPPIQTKGLTTLETFMDTLPDVKTTCITLLVLWTLSREPDDRRPLGHFPDIHFVEEAPRRSIEAFRQRLNQISHDIRQRNKCLPIPYYY 689
BLAST of Arachidonate 5-lipoxygenase vs. TrEMBL
Match: A0A0X3NXQ1 (Allene oxide synthase-lipoxygenase protein (Fragment) OS=Schistocephalus solidus OX=70667 GN=AOSL PE=4 SV=1) HSP 1 Score: 726.087 bits (1873), Expect = 0.000e+0 Identity = 369/745 (49.53%), Postives = 510/745 (68.46%), Query Frame = 2
Query: 80 NFVNVLKMGSNKSKIAFEICIVSGDYKEIEDPPSIFMVLFDEQQRQSPTLSYTNLIQDDEGKIQAKFSIENKPWCKIRNFNKLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDPFSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAINQRRLTDLYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLEGWSVKHVIKARRLYIVDFKIMDGLNCKPDRIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIYVCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXE-NTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLP--SDPTERT-----TDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQSVLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMDPSILPNYPGV 2290
+ + MG SK+AFE+C+ S DY+ PS+ MV+FD QSPT++ NL Q++ QAKF+I+ + W K + F +L HIEFWR++ W+L++V+IRDRRHGL G W+YFFFPVH WI Y+ +DCETF ++PF E R +I +Q++ + QR+ G PVE+ VP E FS KW+I+ L+ I Q L++ Y + W SL++L LY Q+NITEP+ L+ +VND+ FGAQRLKGCNP V++LCT +P + E EW+KPHLEGW+++ I A+RLYIVDF IM GL+C+P R++ AP+ALFF+TE++QLKPLAI + S++ L+FL +D WL AK+WFN+ADACHHMI GRL+ ++LE+IYV MRRN QSHPIYQLLAPHF + L V+ KLK+ IF K GW+A+NIQLSKKGIKQL+KRA+K W FDV AN++ + ESR VYD K L NYPYRDDA L++ F F+ K +++FYP G DLL D ELQ WR+ELSDPM+DGG+GI+GIP G+ +NGV++ K Y+ N S+ S TSV+F + E+ S + GI+HL +IV GS LRLPM+DEYG+ +Y L L G P SD ER+ + I LD+ + ALP++ T+++++ R+ +RV QSVLGK+D ++I P A+ LLE+F+K LE + N+ RD++H+Y+ +DP ++PN+PG+
Sbjct: 10 SLIQTWPMGETNSKVAFELCVSSEDYQGNNLGPSVNMVMFDANNAQSPTIAIANLFQNEVDFKQAKFTIDLQSWSKPKIFGRLHHIEFWRTDGGPFASPWFLDRVVIRDRRHGLTGEWDYFFFPVHDWILPDAQYVVHDCETFLPQDEPFPELRDAQIARRQQLLTFTQRAKGLPVEILEVPTTELFSYDAKWNIEELVLELIEQAGLSEKYSSEMPWDSLDSLGALYKQHNITEPLSLSYWMVNDVAFGAQRLKGCNPFVIELCTNLPPSMECIREWIKPHLEGWTLQQTIVAKRLYIVDFAIMRGLHCRPGRVLAAPLALFFYTEKRQLKPLAIHMDADSSDRRLLFLFTDSSEDWLQAKLWFNLADACHHMIAGRLLTHLLLESIYVSMRRNLAQSHPIYQLLAPHFRSLLAVNSKLKDWIFDK-GWIARNIQLSKKGIKQLLKRAFKQWRFDVKANVYAECESRGVYDRKGLGNYPYRDDAFLVYGIFDRFIVKFLRIFYPRGPVDLLADVELQAWRKELSDPMEDGGLGIEGIPCGKARIRNGVVRPV---GKNYSS-NESMIS---DTSVDFVYGLTTIEETKSFVLGIIHLLVIVAGSALRLPMFDEYGFTPHYPLSLFGQPPFHSDYGERSRRMNGSSVSGISSSSGLDRTIAALPNQRMTVEMILFTRVCSRVRQSVLGKYDSNFIVDPRAVALLEEFQKNLEEASQQVAAHNRMRDLHHKYFALDPLVMPNFPGI 746
BLAST of Arachidonate 5-lipoxygenase vs. TrEMBL
Match: A0A4Z2DLH6 (Allene oxide synthase-lipoxygenase protein OS=Schistosoma japonicum OX=6182 GN=EWB00_011208 PE=4 SV=1) HSP 1 Score: 705.671 bits (1820), Expect = 0.000e+0 Identity = 341/738 (46.21%), Postives = 500/738 (67.75%), Query Frame = 2
Query: 101 MGSNKSKIAFEICIVSGDYKEIEDPPSIFMVLFDEQQRQSPTLSYTNLIQDDEGKIQAKFSIENKPWCKIRNFNKLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDPFSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAINQRRLTDLYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLEGWSVKHVIKARRLYIVDFKIMDGLNCKPDRIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIYVCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQD--------QNLDQVVLALPDKHSTLDVLISVRMAARVTQSVLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMDPSILPNYPGV 2290
MG +KIAFE+C+V+GDY E P + MV+FD + QSPT++ ++ Q+D QAKF+I+ + W ++ F +L HIEFW + ++ W+L++VIIRDRR G+ W+YFFFPVH+WI Y+ +DCE + +PF E R EE+ + + F+ +QRS G PVE++ +P E FS +WDI+ L+ I + L D Y + W+SL+ L N Y +YNITEP+ L ++NDICFGAQR++GCNP +++C +P +FE+ LKP+LEGW+++ ++ A RLYI+DF IM GL+CK R + AP+A+FF+TE++QL PLAIQL ++S + N + LP+DP WL AK+W N+ADACHHMIVGRL+ +ILE+IYV +RRN QSHPIY L+APHF + L V++KLKE F ++GW+++NIQLS+KGIKQL++RA+K W FDVNANI+++LESR VYD L NYPYR+DALLI++ FV V+LFYPNG + ++ D ELQ+WR E++ PM++GG+G+ GV ST+ + ++ + N ++ T+ E++V L GIL+L +IV GS LRLPM+DEYG+P Y L L G+ P D + L D L++V+ +LP++ T++ ++ R+++RV QSVLG+F+ D+I++ +A+ LL+ FR QLE + I+ +N RD++HQY +DPS++PNYPG+
Sbjct: 1 MGGTSTKIAFEVCVVTGDYSGDELGPGVNMVMFDHRGNQSPTITLDSIFQNDIDYTQAKFTIDLQAWSRLTVFKRLHHIEFWCTTQSYPPPAWFLDRVIIRDRRFGMTAEWKYFFFPVHKWINQDHQYVVHDCEAWIPQLEPFPELREEEVGRRMQFFTFFQRSKGLPVELQEIPPSELFSTDSRWDIEPLVLEVIERTGLADEYTSEQSWNSLDTLGNFYKKYNITEPVSLQFWMMNDICFGAQRVRGCNPFTIQICRTLPKSFEMAANTLKPYLEGWTLRQMVSANRLYILDFHIMQGLSCKRGRELCAPLAIFFYTEKRQLTPLAIQLNRNSTDTNAIILPTDPSSIWLQAKLWVNLADACHHMIVGRLLTHLILESIYVSLRRNMSQSHPIYHLVAPHFRSILPVTRKLKEWTF-ENGWISRNIQLSRKGIKQLLRRAFKKWRFDVNANIYRELESRGVYDPNGLGNYPYREDALLIYHTLEQFVSSYVRLFYPNGTEQIIHDNELQSWRHEIASPMEEGGLGLI----------GVPGSTI-KIRSNSRSNNCLYDSPTEEIFGLITV----EEVVKFLMGILYLDVIVAGSALRLPMFDEYGFPAQYPLSLFGSPPLDRNAFSDGTLHGVHDSVFSFSSVHGLERVLASLPNRQMTVETVLYTRLSSRVQQSVLGQFESDFIFKKKAVILLDQFRHQLEEMAKQIDMNNTARDMHHQYRALDPSLMPNYPGI 722
BLAST of Arachidonate 5-lipoxygenase vs. TrEMBL
Match: A0A183STJ9 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS7547 PE=4 SV=1) HSP 1 Score: 705.286 bits (1819), Expect = 0.000e+0 Identity = 350/737 (47.49%), Postives = 484/737 (65.67%), Query Frame = 2
Query: 101 MGSNKSKIAFEICIVSGDYKEIEDPPSIFMVLFDEQQRQSPTLSYTNLIQDDEGKIQAKFSIENKPWCKIRNFNKLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDPFSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAINQRRLTDLYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLEGWSVKHVIKARRLYIVDFKIMDGLNCKPDRIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIYVCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLP--SDPTERT-----TDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQSVLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMDPSILPNYPGV 2290
MG SK+AFE+C+ S DY+ PS+ MV+FD QSPT++ NL Q++ QAKF+I+ + W K + F +L HIEFWR++ W+L++V+IRDRRHGL G W+YFFFPVH WI Y+ +DCETF ++PF E R +I +Q++ + QR+ G PV +W+I+ L+ I Q L++ Y + W SL++L LY Q+NITEP+ L+ +VND+ FGAQRLKGCNP V++LCT +P + E EW+KPHLEGW+++ I A+RLYIVDF IM GL+C+P R++ AP+ALFF+TE++QLKPLAI + S++ L+FL +D WL AK+WFN+ADACHHMI GRL+ ++LE+IYV MRRN QSHPIYQLLAPHF + L V+ KLK+ IF K GW+A+NIQLSKKGIKQL+KRA+K W FDV AN++ + ESR VYD K L NYPYRDDA L++ F F+ K +++FYP G DLL D ELQ WR+ELSDPM+D G G E G +S + + TSV+F + E+ S + GI+HL +IV GS LRLPM+DEYG+ +Y L L G P SD ER+ + I LD+ + ALP++ T+++++ R+ +RV QSVLGK+D ++I A+ LLE+F+K LE + N+ RD++H+Y+ +DP ++PN+PG+
Sbjct: 1 MGETNSKVAFELCVSSEDYQGNNLGPSVNMVMFDANNAQSPTIAIANLFQNEVDFKQAKFTIDLQSWSKPKIFGRLHHIEFWRTDGGPFASPWFLDRVVIRDRRHGLTGEWDYFFFPVHDWILPDAQYVVHDCETFLPQDEPFPELRDAQIARRQQLLTFTQRAKGLPV---------------EWNIEELVLELIEQTGLSEKYSSEMPWDSLDSLGALYKQHNITEPLSLSYWMVNDVAFGAQRLKGCNPFVIELCTNLPPSMECIREWIKPHLEGWTLQQTIVAKRLYIVDFAIMRGLHCRPGRVLAAPLALFFYTEKRQLKPLAIHMDADSSDRRLLFLFTDSSEDWLQAKLWFNLADACHHMIAGRLLTHLLLESIYVSMRRNLAQSHPIYQLLAPHFRSLLAVNSKLKDWIFDK-GWIARNIQLSKKGIKQLLKRAFKQWRFDVKANVYAECESRGVYDRKGLGNYPYRDDAFLVYGIFDRFIVKFLRIFYPRGPVDLLADVELQAWRKELSDPMED------GGLGIEGIPCGKARSMI-----------------SDTSVDFVYGLTTIEETKSFVLGIIHLLVIVAGSALRLPMFDEYGFTPHYPLSLFGQPPFHSDYGERSRRMNGSSVSGISSSSGLDRTIAALPNQRMTVEMILFTRVCSRVRQSVLGKYDSNFIVDLRAVALLEEFQKNLEEASQQVAAHNRMRDLHHKYFALDPLVMPNFPGI 698
BLAST of Arachidonate 5-lipoxygenase vs. TrEMBL
Match: A0A5K4F252 (Putative 12-lipoxygenase OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 703.36 bits (1814), Expect = 0.000e+0 Identity = 339/740 (45.81%), Postives = 496/740 (67.03%), Query Frame = 2
Query: 101 MGSNKSKIAFEICIVSGDYKEIEDPPSIFMVLFDEQQRQSPTLSYTNLIQDDEGKIQAKFSIENKPWCKIRNFNKLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDPFSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAINQRRLTDLYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLEGWSVKHVIKARRLYIVDFKIMDGLNCKPDRIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIYVCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQD--XXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPL--------QIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQSVLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMDPSILPNYPGV 2290
MG +KIAFE+C+V+GDY E P + MV+FD QSPT++ N+ Q+D QAKF+I+ + W +++ F +L HIEFW + ++ W+L++VIIRDRR G+ W+YFFFPVHQWI Y+ +DCE + +PF E R EE+ + F+L+QRS G PVE++ +P E FS +WDI+ L+ I + L + Y + W SL+ L N Y +YNITEP+ L ++NDICFGAQR++GCNP +++C +P +FE+ LKP+LEGW+++ ++ A RLY++DF IM GL+CK R + AP+A+FF+TE++QLKP+AIQL ++SN+ + + LP+DP WL AK+W N+ADACHHMIVGRL+ +ILE+IYV +RRN QSHPIY L+APHF + L V+KKLKE F ++GW+++NIQLS+KGIKQL++RA+K W FDVNANI+++LESR V+D +L NYPYR+DA+LI++A F V+LFYP G + ++ D ELQ+WR E++ PM++ G+ + N L++ L D ++ G +N E++V L GIL+LSIIV GS LRLPM+DEYG+P Y L L G+ P D + L Q LD+V+ +LP++ T++ ++ R+A+RV QSVLG+++ D+I++ +A+ LL+ FR LE + ++ +N RD++HQY +DPS++PNYPG+
Sbjct: 1 MGGTSTKIAFEVCVVTGDYSGDELGPGVNMVMFDHCGNQSPTITLDNIFQNDVDYTQAKFTIDLQAWSRLKVFKRLHHIEFWCTTQSYPPPAWFLDRVIIRDRRFGMTAEWKYFFFPVHQWISQDHQYVVHDCEAWIPQLEPFPELREEEVSRRMHFFTLFQRSKGLPVELQEIPPSELFSSDSRWDIEPLVLEVIERTGLAEEYTSEESWDSLDTLGNFYKKYNITEPVSLQFWMMNDICFGAQRIRGCNPFTIQICRTLPKSFEMVANSLKPYLEGWTLRQMVSANRLYLLDFHIMQGLSCKRGRELCAPLAIFFYTEKRQLKPIAIQLNRNSNDASGIILPTDPTSIWLQAKLWVNLADACHHMIVGRLLTHLILESIYVSLRRNVSQSHPIYHLVAPHFRSILPVTKKLKEWTF-ENGWISRNIQLSRKGIKQLLRRAFKKWRFDVNANIYRELESRGVFDPNSLGNYPYREDAMLIYHALEQFTSNYVRLFYPGGTEQIIHDNELQSWRHEIASPMEEGGLGLVGVPGSTIKLRTNSGLENCL-HDNVTEEIFG----------------LNTVEEVVKFLMGILYLSIIVTGSALRLPMFDEYGFPAQYPLSLFGSPPLDRNTFSDGTLNGAHGSVFSFSNVQGLDRVLASLPNRQMTVETVLYTRLASRVQQSVLGQYESDFIFKKKAVVLLDQFRHDLEDVARQVDINNGARDMHHQYRALDPSLMPNYPGI 722
BLAST of Arachidonate 5-lipoxygenase vs. TrEMBL
Match: A0A4E0RKH3 (Arachidonate 5-lipoxygenase OS=Fasciola hepatica OX=6192 GN=D915_007426 PE=4 SV=1) HSP 1 Score: 694.115 bits (1790), Expect = 0.000e+0 Identity = 335/739 (45.33%), Postives = 490/739 (66.31%), Query Frame = 2
Query: 101 MGSNKSKIAFEICIVSGDYKEIEDPPSIFMVLFDEQQRQSPTLSYTNLIQDDEGKIQAKFSIENKPWCKIRNFNKLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDPFSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAINQRRLTDLYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLEGWSVKHVIKARRLYIVDFKIMDGLNCKPDRIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIYVCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDP---------TERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQSVLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMDPSILPNYPGV 2290
MG N +KIAFE+C+VSGDY E P + MV+FD QSPT++ ++ Q++ QAKF+I+ +PW K++ F +L HIEFW + ET W+L++VIIRDRR G+ W+YFFFPVHQWI Y+ +DCE++ +DPF + R E+ + + F+L+QR+ G PVE+ ++P E F+ +W+I++ L+ I + L Y + WSSL+ L + Y +YN+TEP+ L +VNDICFGAQR++GCNP +++LC ++P++F W+KPHLEGW++K + A RLY++D++IM GL+CK R + AP+ L TE++QL+P+AIQL + + +FLP+DP WL AK+W N++DACHHMIVGRL+ MILE++YV +RRN QSHPIYQLLAPHF + L V+ KLKE F ++GW+A++IQL+ KGIKQL+KRA+K W FD+ AN++++LESR VY+ L NYPYR DALLIH +V K V+ YP G +DLL D ELQ+WR E++ PM++ GG ST R+ A S F V TI+ + V L G+LHL +IV GS LRLPM+DEYG+P +Y L L G PSDP + + + +++V +LP++ T+++++ R+A+RV QSVLG+++ D++++ +A+ +LE+FRK L I+ +N +RD++HQY +DPS++PNYPG+
Sbjct: 13 MGGNSTKIAFEVCVVSGDYTGDELGPGVNMVMFDSVGNQSPTITLASIFQNESDYTQAKFTIDCQPWSKLKVFRRLHHIEFWCTTETDPPPAWFLDRVIIRDRRFGMTAEWKYFFFPVHQWISPDHQYVVHDCESWLPVQDPFPDLRDTELTMRLQFFTLFQRAKGLPVELRDIPPSEYFTSDSRWNIESLVLEVIERYGLAPEYTSEQPWSSLDELGSFYKKYNVTEPMSLQFWMVNDICFGAQRIRGCNPFMIRLCQQLPESFAAAATWIKPHLEGWTLKQLTSANRLYLLDYEIMQGLSCKRGRALCAPLVLLLHTEKRQLRPIAIQLRHEPKDTSPIFLPTDPVHIWLQAKLWVNLSDACHHMIVGRLLTHMILESVYVSLRRNLAQSHPIYQLLAPHFRSILPVTHKLKEWTF-ENGWIARSIQLNHKGIKQLLKRAFKQWRFDIQANLYRELESRGVYNPHGLGNYPYRQDALLIHRILEKYVNKFVRYVYPRGTEDLLQDTELQSWRHEIASPMEE---------GGLGLVGVPGSSTKFRNPGDA---MSEFGYPDFPEVFGLITID---ETVKFLVGLLHLIVIVAGSALRLPMFDEYGFPAHYPLSLFGAPPSDPGVSESGLLNSRVASSASSLSNILGTERIVASLPNRQMTVEIVLYSRLASRVQQSVLGQYESDFMFKQQAVTILEEFRKDLAEAAQQIDSNNLSRDMHHQYRALDPSVMPNYPGI 735
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Cavefish
Match: alox5a (arachidonate 5-lipoxygenase [Source:NCBI gene;Acc:103035394]) HSP 1 Score: 160.614 bits (405), Expect = 1.877e-40 Identity = 154/668 (23.05%), Postives = 293/668 (43.86%), Query Frame = 2
Query: 323 KLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDP---FSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAIN-QRRLTDLYGDQ------SFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLN------CKPDRIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRREL-SDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQS--VLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMDP 2263
K+E ++W + DWY + + ++ +Y FP +W+ + I D +D + R +E+ +Q+ F + GFP+ ++ + + + ++D + +N + + +LY +Q S W + ++ + T + D FG Q L GCNP++++ CT+IP F VT E + LE +++ +KA LYI D++IM+ + C P + + AP+ L + + ++ P+AIQL+QS E + ++LPSD WLLAK+W +D H V L+ ++ ++ + M R HP Y+LL PH + ++ K +E + ++G + G QLV++A K +++ + + +++R + + L Y YRDD + A +FV M+ ++Y + + + DEE+Q + +++ S MQD F ++ E LV LT ++ + + + YD + + N P PT R P + Q ++ +V +LPD+ + L +V ++ ++ LG + ++ + K LE FR++L + I+ N+ + + YY + P
Sbjct: 99 KIEKKKYWVHD------DWYCKYITVKTPSG------DYIEFPCFRWLVDDKEVILRDGRARLPQDDKTRVAKQHRRKELETRQKTFRWKEWHPGFPMSIDAKAHK-ELPRDIQFDSEKGVDFILNYSKAIENLYVNQFMHMFQSSWGDFSDFERIFMRIKNTISEYVMQHWKEDFMFGYQFLNGCNPVMIQKCTQIPKKFPVTHEMVADCLERDLTLEEELKAGNLYIADYEIMEDITANATDPCTP-QYLAAPICLLYKNSQNKILPIAIQLSQSPGEDSPIYLPSDDQHDWLLAKIWVRSSDFHVHQTVTHLLRTHLISEVFGIAMFRQLSAVHPAYKLLLPHIRFTIAINTKAREQLICENGLFDKANGTGGGGHVQLVQKAMKTFTYK-SLCFPESIKARGMESQEELPYYYYRDDGNKVWEAIKSFVTDMINIYYASD-ETVQEDEEIQAFVKDVCSFGMQDFDY-----------------------------------------CEFPKSVQTLEQLVEYLTVVI-FTASAQHAAINFGQYDWCSW--------IPNAP--PTMRNPPPTKKGQ-TDMKLIVESLPDRGRSCWHLGAVWALSQFQENELFLGMYPDEHFIEKPVKKALETFREKLTEVSKLIKNRNEGKKL--PYYYLSP 695
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Cavefish
Match: alox12 (arachidonate 12-lipoxygenase, 12S-type [Source:NCBI gene;Acc:103037595]) HSP 1 Score: 159.073 bits (401), Expect = 4.769e-40 Identity = 151/665 (22.71%), Postives = 287/665 (43.16%), Query Frame = 2
Query: 323 KLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDPFSET---RLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXX-----KFSKSEKWDIDNA---KLDAINQRRLTDLYGDQSFWSSLENLHNLYSQYNITEPIG--LTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLNCKPDRIM----PAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLI-NQMILENIYVCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPD-KHSTLDVLISVRMAARVTQSV-LGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYC 2254
+LE +W ++ +W+ V++ + G FP + W+ G + K +D + R E++ +QE + + G P ++ E E +F+ ++ D + + L ++ ++L +G W+ L++ ++ + + PI D FG Q + G NP +++ T++P NF V+ + ++ L ++ +KA +Y+VD I+DG+ R M APM L + E L P+AIQL Q+ + +FLP+DPP++WLLAKMW A+ ++ L+ +++E I V R+ HPIY+LL PH L ++ + + + S +G + + G+ L +R YK ++ + D R V LK Y YRD +L++ +A +V MV L+Y + ++ D ELQ W +++ D+ + D+ N + E+ V+LL+ ++ +S ++ N +P+ P P + + ++ LPD S + + I+ + ++ +G++ + +PEAL+ +E FR+ L+ I I + N+ ++ + Y C
Sbjct: 67 RLEKQRYW------VEDNWFCRYVLVTPKEGGSTQT-----FPCYCWLVGDMKVEVREGTAKKLSDDVLPKELAHRKAELKQRQETYRWIAWAPGIPKCIDAKTEAELPQDDRFANEKRSDFEGSLHYALLELSLKKLAIRFGKS--WNDLDDFKRIF--WKLRSPIAEYAMEHWKEDWFFGYQFMNGSNPRMIQKVTKLPTNFPVSGDMVQAFLSPNTTLDKELKAGNVYLVDHGIVDGIPANVIRNMQQYIAAPMCLLYEHPESGLIPIAIQLEQNPGKDTPIFLPNDPPLAWLLAKMWVRHAEFQVFQLLSHLLRTHLVVEVICVSTLRHLPAVHPIYKLLTPHLKYTLEINCRGRTQLISPEGIFKRVVSTGGTGLLVLAQREYKILTYRSLQPTY-DFLDRGVTKLK---KYFYRDYSLMLWDAIHKYVSSMVSLYY-SSDSEVQQDSELQAWIKDIV------------------------------DEGFVDV----------PEFGLPNELKGKEEFVTLLSVVIFISTA------------QHAATNNGQFDWCAWVPNTPCTMRQPPPNDKDAVTMGLIMDTLPDISQSCVQMAITWHLGRAQPDAIPMGQYAEQFFTEPEALQAIESFRQDLKDIDEQIVKQNEGLELQYLYLC 659
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Cavefish
Match: alox5a (arachidonate 5-lipoxygenase [Source:NCBI gene;Acc:103035394]) HSP 1 Score: 157.532 bits (397), Expect = 7.366e-40 Identity = 120/468 (25.64%), Postives = 226/468 (48.29%), Query Frame = 2
Query: 323 KLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDP---FSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAIN-QRRLTDLYGDQ------SFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLN------CKPDRIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRREL-SDPMQD 1669
K+E ++W + DWY + + ++ +Y FP +W+ + I D +D + R +E+ +Q+ F + GFP+ ++ + + + ++D + +N + + +LY +Q S W + ++ + T + D FG Q L GCNP++++ CT+IP F VT E + LE +++ +KA LYI D++IM+ + C P + + AP+ L + + ++ P+AIQL+QS E + ++LPSD WLLAK+W +D H V L+ ++ ++ + M R HP Y+LL PH + ++ K +E + ++G + G QLV++A K +++ + + +++R + + L Y YRDD + A +FV M+ ++Y + + + DEE+Q + +++ S MQD
Sbjct: 69 KIEKKKYWVHD------DWYCKYITVKTPSG------DYIEFPCFRWLVDDKEVILRDGRARLPQDDKTRVAKQHRRKELETRQKTFRWKEWHPGFPMSIDAKAHK-ELPRDIQFDSEKGVDFILNYSKAIENLYVNQFMHMFQSSWGDFSDFERIFMRIKNTISEYVMQHWKEDFMFGYQFLNGCNPVMIQKCTQIPKKFPVTHEMVADCLERDLTLEEELKAGNLYIADYEIMEDITANATDPCTP-QYLAAPICLLYKNSQNKILPIAIQLSQSPGEDSPIYLPSDDQHDWLLAKIWVRSSDFHVHQTVTHLLRTHLISEVFGIAMFRQLSAVHPAYKLLLPHIRFTIAINTKAREQLICENGLFDKANGTGGGGHVQLVQKAMKTFTYK-SLCFPESIKARGMESQEELPYYYYRDDGNKVWEAIKSFVTDMINIYYASD-ETVQEDEEIQAFVKDVCSFGMQD 520
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Cavefish
Match: ENSAMXT00000046181.1 (pep primary_assembly:Astyanax_mexicanus-2.0:15:18032762:18048964:-1 gene:ENSAMXG00000037527.1 transcript:ENSAMXT00000046181.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 152.525 bits (384), Expect = 2.758e-38 Identity = 121/530 (22.83%), Postives = 234/530 (44.15%), Query Frame = 2
Query: 686 INQRRLTDLYGDQS------FWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLEGWSVKHVIKARRLYIVDFKIMDGLNCKPDRI----MPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQSV--LGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDI 2236
IN+R+L L +WS L L N+ L D FG Q L G NP V++ C +PDNF VT ++ +E S+ I ++++ D+ ++DG+ + + AP+ L + + +L P+AIQ Q + E N +F+P D WLLAK++ AD H + L+ +L +Y +C N HP+Y+LL PH L ++ +E + +KDG + + +SK+G+ ++++A + + + +D+E+R LK + N+ YRDD + + T +V +++ +YP+ ++ D ELQ W ++ L L +K+ N + ++L+ +T ++ ++ S L +D G+ +P+ P + P +++ ++ LPD + ++ + +V R + LG Y +P+ + ++ + +L+ + I++ N+ +D+
Sbjct: 98 INERQLVYLREGTGKTILHIWWSQLA--EGLPHTLNVDNVKDLPEHWKEDEFFGYQFLNGANPTVVRQCRRLPDNFPVTDSMVQASMEDSSLTTEIMKGKIFLCDYALLDGVQANTVNLRRQYVGAPLCLLYQNKNDKLIPIAIQCNQPAGEENPIFVPGDSKEDWLLAKLYVRAADFLIHQLDAHLLRTHLLVEVYTLCTLHNLPAVHPVYKLLIPHTQYTLHINTLARERLLAKDGLIIKYSAVSKEGLNTVLRKALDSLQYSALC-LPEDIEAR---GLKDIPNFFYRDDGQEVWDIITKYVGGVLQFYYPSD-IEVAADVELQKWIGDI-----------------------FLHGFLGNEKS-----------------NIPKAFRSKDELIKFVTMVI-FTMTAQHSALNSGQFDFGGW-----------IPNSPPSLQSPPRKVKGRATEGTLLHTLPDVNVSVQSMAAVWGLTRKPEEFVSLGNHTEQYFTEPDVVAVINVMKDELKQLSEKIKKRNEGKDL 568
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Cavefish
Match: ENSAMXT00000046619.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000924.1:26844:39215:1 gene:ENSAMXG00000029118.1 transcript:ENSAMXT00000046619.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 146.747 bits (369), Expect = 2.479e-36 Identity = 129/527 (24.48%), Postives = 241/527 (45.73%), Query Frame = 2
Query: 692 QRRLTDLYGDQSFWSSLENLHNLYSQYNITEPIGLTNSI----VNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHL-EGWSVKHVIKARRLYIVDFKIMDGLNCKPDRIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQS--VLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQY 2248
+ +L + ++ W+++ L ++ + +NI P+ LT + + D FG Q L G NP ++K C+E+P+NF VT + +K L E +++ I+ +++ D+K + + + AP+ L F T E +L P+AIQL Q N +FLPSD WLLAK++ A+ H + L+ +L ++ + + RN HP+++LL PH L ++ + + S DG +Q + K + + +KRA + ++ + + ++ R V + + NY YRDD L + N +FV ++ +Y +D+ D+ELQ W E+ + E +K G +S + ++ + +F+ Q + +NN + +D + +P+ P+ P + D D ++ LPD +TL+++ V + +R + LG F + L L+ F++ L + IE+ NK D+ + Y
Sbjct: 144 ELKLGTEFNNKDRWNNIGEL-SIIALFNIFMPV-LTEYVEKNWMRDDFFGYQFLNGLNPTMIKRCSELPENFPVTNDMVKRSLPEKSTLEMEIEKGNIFLCDYKRLK-------QYLSAPLCLLFSTPENKLTPIAIQLDQKPGARNPIFLPSDNEKDWLLAKIFVRGAEFNEHELNFHLLRTHLLGEVFTMAVLRNLPSCHPLFKLLVPHVRYNLQINIMARNLLVSPDGVFSQYTAIGNKPMTEFLKRAASSLTYS-SLCLPDNIRERGV---EKIPNYYYRDDGLDLWNIIYSFVHGVLSFYY--NDEDVQKDKELQAWIDEIVE-----------NCFQEKSKTGFPQSFVTVEELSKFVTMVIFTVSAQHA-----AVNNGQ-------------------------FDFGAW-----------MPNFPSSLQCPPPIRKGDTTDDTILKTLPDVSTTLNIMAVVYLLSRESSDRYPLGYFPEALFREDTPLDLIAKFQEDLRVLDKKIEKRNKKLDLPYTY 603
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004004.1 (pep scaffold:Pmarinus_7.0:GL480279:6425:26929:-1 gene:ENSPMAG00000003663.1 transcript:ENSPMAT00000004004.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 153.295 bits (386), Expect = 4.412e-40 Identity = 92/282 (32.62%), Postives = 146/282 (51.77%), Query Frame = 2
Query: 815 DICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLNCKPD-----RIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNW 1639
D FG Q L GCNP+V K CTEIP VT E ++ LE S++ K ++I D+ IMDG+ + + AP+ L + K+L P+AIQL Q+ N +FLP+D W+LAKMW D H V L++ ++ ++ + M R HPI++LL H + ++ K +E + K G + G QLV+RA K ++ + K+++ R V ++L Y YR D + N FV++++ ++YP+ K + +D E+Q W
Sbjct: 10 DFMFGYQFLNGCNPVVFKKCTEIPAKLPVTHEMVEACLERSISLEEEAKQGNIFIADYSIMDGIQANSTDPCTMQYVAAPICLLYNNVNKKLVPIAIQLQQTPGSDNPIFLPTDSNNDWMLAKMWARSCDFHVHQTVSHLLHTHLISEVFGIAMFRQLPAVHPIFKLLVHHTRFTIAINTKAREELICKCGLFDKANATGGGGHVQLVQRAVKELKYE-HLQFPKNIQMRGVDSEESLPYYFYRKDGQQVWNCTAQFVKEVLAVYYPDD-KTVKMDSEVQAW 289
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006734.1 (pep scaffold:Pmarinus_7.0:GL476592:109075:119405:-1 gene:ENSPMAG00000006039.1 transcript:ENSPMAT00000006734.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 140.584 bits (353), Expect = 3.398e-35 Identity = 129/514 (25.10%), Postives = 220/514 (42.80%), Query Frame = 2
Query: 719 DQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHL-EGWSVKHVIKARRLYIVDFKIMDGL-----NCKPDRIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCG---SGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKH-STLDVLISVRMAARVTQSVLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKN 2227
D W+ +E+ L+++ +++ D FG Q + G NP+++K CT++P+NF VT +K L S++ IK +YIVD++ +DG+ N + APM L + ++ QL P+AIQL Q + N +FLPSD W+LAKMW D H I L+ +L ++ + R HP++++L N LV+ K+ G + + + G +L + K ++ + I KD+ R V DL + Y YRDDA I FV K++ +FY +D+ D ELQ + D+N ++ S N N VS L+ L + + +C S + +D Y + +P+ P+ P + ++ ++ LPD S + + ++ ++ + LG F + P+ALK +++++ LE I I NK
Sbjct: 89 DGMSWTKIEDFRRLFTRVKSEIAEYISHHWDQDWFFGYQFMNGVNPVLIKRCTKLPENFPVTTNMVKSSLVRECSLEDEIKHGNVYIVDYQCLDGIPGNTFNHSKPFYLAAPMCLLYVNKQGQLLPIAIQLQQKPSADNPIFLPSDSAHDWMLAKMWVRSMDFQIHEISTHLLKTHLLAEVFTMATLRQLSTMHPVHKILP----NTLVIFVKVNFYTCGLCGTILPALSIGGGGHFKLGGKGLKALTYR-HLCILKDVADRGVGDLPS---YYYRDDACSIWKTIHKFVDKILAIFY-KSDEDVRGDAELQAF--------------------------------------VDDINHALLS-------NPSNGFPTKFTCVSELSEFLTMVVFLCSAQHSAVNYGQFDFYAW-----------IPNAPSAMRKPPPATKGVVDMPFILQTLPDVQVSCVSMALTWVLSRSAAEKTLGTFQDEPFQNPDALKAMQEYQSDLEVITKSIVERNKT 537
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Sea Lamprey
Match: zgc:152891 (zgc:152891 [Source:ZFIN;Acc:ZDB-GENE-061013-229]) HSP 1 Score: 133.265 bits (334), Expect = 6.757e-33 Identity = 127/523 (24.28%), Postives = 214/523 (40.92%), Query Frame = 2
Query: 719 DQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHL-EGWSVKHVIKARRLYIVDFKIMDGL-----NCKPDRIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQ-LLAPHFTN--------CLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCG---SGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKH-STLDVLISVRMAARVTQSVLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKN 2227
D W+ +E+ L+++ +++ D FG Q + G NP+++K CT++P+NF VT +K L S++ IK +YIVD++ +DG+ N + APM L + ++ QL P+AIQL Q + N +FLPSD W+LAKMW D H I L+ +L ++ + R HP+++ ++ P N C V + + +I + L + L I KD+ R V DL + Y YRDDA I FV K++ +FY +D+ D ELQ + D+N ++ S N N V+ L+ L + + +C S + +D Y + +P+ P+ P + ++ ++ LPD S + + I+ ++ + LG F + P+ALK +++++ LE I I NK
Sbjct: 71 DGMSWTKIEDFRRLFTRVKSEIAEYISHHWDQDWFFGYQFMNGVNPVLIKRCTKLPENFPVTTNMVKSSLVRECSLEDEIKHGNVYIVDYQCLDGIPGNTFNHSKPFYLAAPMCLLYVNKQGQLLPIAIQLQQKPSADNPIFLPSDSAHDWMLAKMWVRSMDFQIHEISTHLLKTHLLAEVFTMATLRQLSTMHPVHKNVVTPFLVNLYPNTFPQCHVHTVTMALSIGGGGHFKLGGKGLKTLTYRHLC--------------ILKDVADRGVGDLPS---YYYRDDACSIWKTIHKFVDKILAIFYKRD-EDVRGDAELQAF--------------------------------------VDDINHALLS-------NPSNGFPTKFTCVAELSEFLTMVVFLCSAQHSAVNYGQFDFYAW-----------IPNAPSAMRKPPPTTKGVVDMPFILQTLPDVQVSCISMAITWVLSRSAAEKTLGTFPDEPFQNPDALKAMQEYQSDLEVITKSIVERNKT 519
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003097.1 (pep scaffold:Pmarinus_7.0:GL477262:63996:70580:-1 gene:ENSPMAG00000002831.1 transcript:ENSPMAT00000003097.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 77.7962 bits (190), Expect = 7.785e-16 Identity = 64/287 (22.30%), Postives = 136/287 (47.39%), Query Frame = 2
Query: 845 GCNPLVLKLCT---EIPDNFEVTVEWLKPHLEGWSVKHVIKARRLYIVDFKIMDG-----LNCKPDRIMPAPMALFFFT----EEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRL-INQMILENIYVCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQN-IQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLK--ALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSD 1657
G NP+++K+C+ ++P + + + +++ ++ RRL+IVD+K + G ++ AP+ L + +L PL IQLT++ E P+ +L AK+ A+ H V L + +++E + + G H + +LL PH T+ + ++ + + + G + + + G +L +++ + F + + ++L +R + + L ++ YRDD + + A+VR++V Y + D+L D+ LQ++ L+D
Sbjct: 1 GANPMLIKVCSSVDQVPPDMRCLLG------QDRTLEELMSERRLFIVDYKALAGESRSRTRGTQEKKFYAPVVLLYREMCPDGTGRLMPLGIQLTRNPRELRWRHTPTSRAWDYLFAKIHVGCAENQMHQFVSHLALTHLLMEPFAIAVHNYLGPKHVLGRLLRPHCTDTIGINYVARHTLIAAVGPLTNSTFAVGTVGGLRLAVASFQRYDF-MEWSFPRELHNRGFDEARDDGLEDFLYRDDGFKLWHVLGAYVREVVCRHY-HTDADVLHDKGLQDFAAALAD 279
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003100.1 (pep scaffold:Pmarinus_7.0:GL477262:65962:71085:-1 gene:ENSPMAG00000002831.1 transcript:ENSPMAT00000003100.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 72.4034 bits (176), Expect = 4.967e-14 Identity = 58/268 (21.64%), Postives = 125/268 (46.64%), Query Frame = 2
Query: 827 GAQRLKGCNPLVLKLCT---EIPDNFEVTVEWLKPHLEGWSVKHVIKARRLYIVDFKIMDGLNC--KPDRIMPAPMALFFFT----EEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRL-INQMILENIYVCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQN-IQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLK--ALNNYPYRDDALLIHNAFTAFVRKMVKLFY 1591
G G NP+++K+C+ ++P + + + +++ ++ RRL+IVD+K + L+ ++ AP+ L + +L PL IQLT++ E P+ +L AK+ A+ H V L + +++E + + G H + +LL PH T+ + ++ + + + G + + + G +L +++ + F + + ++L +R + + L ++ YRDD + + A+VR++V Y
Sbjct: 16 GVSTAAGANPMLIKVCSSVDQVPPDMRCLLG------QDRTLEELMSERRLFIVDYKALAVLHIPLHQEKKFYAPVVLLYREMCPDGTGRLMPLGIQLTRNPRELRWRHTPTSRAWDYLFAKIHVGCAENQMHQFVSHLALTHLLMEPFAIAVHNYLGPKHVLGRLLRPHCTDTIGINYVARHTLIAAVGPLTNSTFAVGTVGGLRLAVASFQRYDF-MEWSFPRELHNRGFDEARDDGLEDFLYRDDGFKLWHVLGAYVREVVCRHY 276
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Nematostella
Match: EDO42509 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1H7]) HSP 1 Score: 267.314 bits (682), Expect = 8.824e-79 Identity = 163/480 (33.96%), Postives = 252/480 (52.50%), Query Frame = 2
Query: 128 FEICIVSGDYKEIEDPPSIFMVLFDEQQRQSPTLSYTNLIQDD--EGKIQAKFSIENKPWCKIRNFNKLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIF--YDCETFKGYEDPFSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAINQRRLTDLYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLEGWSVKHVIKARRLYIVDFKIMDGLNCKPDRIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRL-INQMILENIYVCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNA 1552
+EI + +GD K +++ L +S ++D +G + + F + N L+ IE WR + + D DWY+E + +++ L E FP H+W+ R I YDC + + P E R E+ K+E++ L +++ G ++W+I K + +LT L D W LE+L N+Y + ++ +P GL N D F QRL+GCNP +++LC EIP+NF VT E ++P L+G +++ I A+R+YI + + + C+ +R++ PMALFF K+L P+AIQL Q E N VFLPSDP +W+LAKM++N ADA H L ++ E I V R SHP+++LLAPHF L ++ + S +GW+ +Q +G+ L ++K W +VN + K+L+ R V D +AL NYPYRDDALLIH
Sbjct: 11 YEIIVKTGDVKGAGTDANVYCALVAVNGTRSRDFHLDCRWKNDFEKGNVDS-FKVANG-----TAIGPLKKIEIWRDDSGLGD-DWYVEWIKVKNI---LEPNSELDVFPCHRWVKAERKLILTKYDCMLPQFDDHP--EQRKRELEEKRELYRLVRKAPGI---------------PKQWNIQQTKYKLAVKCKLTKLTSDP--WEKLEDLDNVY-KGSMGKPYGLYN-WKEDSAFSRQRLQGCNPTMIELCREIPENFAVTSEMVEPFLDGMTLEEAINAKRIYICNLITLSDIVCRFNRLISKPMALFFVNNSKELMPIAIQLFQKPAEDNPVFLPSDPKYTWMLAKMYYNNADAAFHQSCTHLGFTHLVAETICVGTHRQVSPSHPVFRLLAPHFLYLLAINSLALARLVSPNGWIDICMQTGAEGLMGLCGASWKKWRMNVNGWLPKELKERGVDDAEALPNYPYRDDALLIHQG 459 HSP 2 Score: 68.9366 bits (167), Expect = 4.869e-12 Identity = 44/149 (29.53%), Postives = 75/149 (50.34%), Query Frame = 2
Query: 1832 EDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQSVLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMDPSILPN 2278
EDL+ +T I+ +S V + YDEY +P NY L G P+ E T E+D ++ +P K TLD+++ ++ + + LG F++ Y Y P K +E+FR++L + ++ NK R + Y ++P +PN
Sbjct: 470 EDLIKTVTSIIFISS-VGHAAANFAQYDEYAFPPNYPAFLRGKPPTSKEELT------EKD-----IIDHIPLKDMTLDIMLVTKILSDRGTNPLGDFEVQYQYDPIGTKAVENFRRKLTEVSAVLDERNKTRPFPYPY--LNPKEIPN 604
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Nematostella
Match: EDO29209 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T4A3]) HSP 1 Score: 157.532 bits (397), Expect = 2.623e-41 Identity = 132/484 (27.27%), Postives = 214/484 (44.21%), Query Frame = 2
Query: 812 NDICFGAQRLKGCNPLVLKLCTEIPDNFE-VTVEWLKPHLEGWSVKHVIKARRLYIVDFKI-------MDGLNCKPDRIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGR-LINQMILENIYVCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQL-SKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQS--VLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKN 2227
+D FG Q + G NPLVL C + P+N + G S+ +K +Y VD+KI M G +R M P+ALF+ + L P+AIQL Q EGN V+ P D P+ WL AKMW AD H ++ R L+ ++ E V R HP+++LL H N L ++ + + KDG +AQ + + + + QL+++ YK ++++ + ++ L+ + + D L Y YRDDAL + NA FVR+ + +FY KD+ D E+Q W ++S NG + L G F N I LV LLT + I C ++ + L++ +P+ P P + +L+ ++LALP K T L + + ++ + GK +PE ++E ++ +L+ I I + N+
Sbjct: 3 DDHVFGMQFMNGVNPLVLDRCDKFPENLAPLDGLVDDLLDRGKSLYREMKDGHIYKVDYKILEEIPRHMQGSGKDKERYMAEPIALFYANKSGDLIPIAIQLYQKPGEGNPVWTPHDAPLDWLYAKMWLKNADIQAHQVIHRLLLTHVMTEPFTVATWRQLPSIHPVHRLLFSHMRNTLALNVTARNELIGKDGILAQTLSIGGGESLVQLMQKFYKKFNWE-DMDVPNLLKRKGLDDKTKLQGYFYRDDALSLWNALKNFVREFLGVFY-KSDKDVTDDTEIQAWLSDIS-------------------TNG-----------FPTLQG-------HQDHKFPNMIRTRYQLVHLLTCV----IFNCTC--------QFAAVNSGQLEMSLFMPNAPCCLHLPPPTTKGVVDLETIMLALPTKKETAVHLGLMHVFSKCAEDEVSFGKNLCCQFQEPEVSTIIERYQNELQKISESIRKRNEG 435
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Nematostella
Match: EDO42294 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S208]) HSP 1 Score: 89.7373 bits (221), Expect = 2.075e-18 Identity = 87/313 (27.80%), Postives = 133/313 (42.49%), Query Frame = 2
Query: 815 DICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEW------LKPHLE-----------GWSVKHVIKARRLYIVDFKIMDGLNCKPDRIMPA-----------PMALFFFTE----EKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLI-NQMILENIYVCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELS 1654
D F RL G NP+ L T D+ V+W L P + V +I +++ F D L PD IM A P+ALF E Q+ PLAIQL S + + V+ P D + W LAK F + D + +V L+ +++E V ++R+ HP+ Q+L H L +K + ++ Q + G QL+ RAY ++ + + + +R + D + +PYRDD LI+ A FV + +Y N R D+ D ELQ E+S
Sbjct: 246 DQVFSEMRLAGLNPMCLTKVTG--DHESNGVKWSELRRRLNPQFDWEKMLQVAASRSAPVSMIINDGSVFVCQFPEFDNLPTVPD-IMEARSFRRMWKPKSPIALFMSRPGRGGEAQVVPLAIQL--DSVQDSPVYTPEDGDL-WTLAKESFEVTDYAYVEMVEHLLKTHLMMEPFCVTIKRHLATDHPLQQILRYHCRGLLPTNKIGLPKLVDDKAYMHQLFAIGNSGAVQLLVRAYMTMTWK-DTDFRGKIRARGMDDTDNVPYFPYRDDGELIYAAIEKFVGGYLNHYYGNDR-DVEDDHELQAMANEMS 550
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Nematostella
Match: EDO33210 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SSW5]) HSP 1 Score: 76.6406 bits (187), Expect = 4.862e-16 Identity = 41/124 (33.06%), Postives = 64/124 (51.61%), Query Frame = 2
Query: 1076 PLAIQLTQSSNEGNLV--------FLPSDPPVSWLLAKMWFNMADACHHMIVGRL-INQMILENIYVCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVK 1420
P+AIQL Q + N V FLP DP +W LAKMWFN A+A +H + +++E+I + R SHPI++LLAP + + ++ + + W+ + L KGI L++
Sbjct: 2 PIAIQLFQEKSANNPVRMSFNNPVFLPGDPWYTWSLAKMWFNHAEAQYHRACALIGFTHLVIESIAIATHRQLSPSHPIFRLLAPFIRDVIAINSFEVNELLAPGSWLDSSTSLGSKGIVDLLQ 125
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Nematostella
Match: EDO44999 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUD1]) HSP 1 Score: 80.4925 bits (197), Expect = 2.428e-15 Identity = 74/313 (23.64%), Postives = 129/313 (41.21%), Query Frame = 2
Query: 752 HNLYSQYNITEPI-GLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHL------------------EGWSVKHVIKARRLYIVDFKIMDGLNCKP-------DRIMP--APMALFFFTEE----KQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADA-CHHMIVGRLINQMILENIYVCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFY 1591
H YS + + + + ++ D CF QRL G NP + T N V + L L + ++ I A ++Y+ + ++GLN + P AP+A+F E K+L P+AIQ G + W AK AD HMI RL + L+ + + F + HP++Q+L H L +K + ++ + + + + + ++V + Y F ++ ++L+ R V D K L YPYRDD +++ FV + ++L+Y
Sbjct: 327 HKKYSAMSALKRVPSIEDNFYKDRCFADQRLAGANPFQIWRVTRSSANVGVGWDTLYEKLLNRDFDWHKAFRKVLETDDNDALDKAIDAGQMYVTFYPELEGLNATKLQDLKFNHTVSPLTAPIAMFVVKEAEPGVKRLTPVAIQ---GDAHGKATVYTPEEGKGWFYAKAMVQRADINVLHMIKKRLKTHLYLDAPCTLVEKYFSEYHPLHQMLRYHCRANLETNKLFELKLYGEKAPIYKALSIDYDNSVRMVNKEYGKMDFS-EIDLVEELKKRGVDDDKLLPYYPYRDDGKKLYSKIKEFVHQYIRLYY 635
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Medaka
Match: alox12 (arachidonate 5-lipoxygenase [Source:NCBI gene;Acc:101174953]) HSP 1 Score: 164.466 bits (415), Expect = 3.322e-42 Identity = 153/665 (23.01%), Postives = 293/665 (44.06%), Query Frame = 2
Query: 323 KLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYD--CETFKGYEDP-FSETRLEEIRHKQEIFSLYQRSSGF-----XXXXXXXXXXXKFSKSEKWDIDNA---KLDAINQRRLTDLYGDQSFWSSLENLHNLYSQYNITEPIG--LTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHL-EGWSVKHVIKARRLYIVDFKIMDGL-----NCKPDRIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLI-NQMILENIYVCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQ-TSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPD-KHSTLDVLISVRMAARVTQSV-LGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQY 2248
+LE +W ++ +W+ V + G + FP ++W G + T K P E R +E++ ++E + ++G ++P++ +F ++ D +++ L ++ ++ T +G W+ LE+ ++ + + PI + D FG Q L G NP +++ C ++PDNF VT ++ + +G ++ +KA +Y++D+ IM+G+ KP + + AP+ L + + L P+AIQL Q+ +FLPSDPP++WLLAKMW A+ ++ L+ +++E V R HPIY+LLAPH L ++ + + + S+ G + + +G+ L +R YK ++ + D R V + NY YRD +L++ +A +FV MV L+YP +D+ D ELQ W R+++ G TQ + K+ +++ E+L +LL ++ ++ N +P+ P P + ++ ++ LPD S + + I+ + ++ LG++ + + AL++++ FRK+L+ I I N D+ + +
Sbjct: 4 RLEKQRYW------VEDNWFCRYVTVEPEDGG-----KMQIFPCYRWFVGDSKIEIREGKATTVKDDTSPKLLEHRKKELQERRETYRWITWAAGIPRCIDAKTEADLPQDARFENEKRSDFEHSLHYALLELSLKKFTIRFGKS--WNDLEDFKRIF--WKLRSPIAEYCMDHWKEDWLFGYQCLNGSNPRMIQRCQKLPDNFPVTAGMVQSSMADGTTLNKELKAGNIYLLDYAIMEGIPTNTIKGKP-QFIAAPLCLLYQHPDDGLIPIAIQLEQTPGLDTPIFLPSDPPLAWLLAKMWVRHAEFQVFQLLSHLLRTHLVVEVFCVATLRQLPAVHPIYKLLAPHQRYTLEINCRGRTQLLSETGIFKRVVSTGGEGLLVLAQREYKVLTYR-SLQPRFDFIDRGVTQIP---NYFYRDHSLMLWDAIHSFVTGMVDLYYPTD-QDVQKDAELQAWIRDVTQ-----------------------------------------EGFTQLPNFGLKSKLSSREELSTLLAVVIFTPTA------------QHAATNNGQFDWCAWVPNTPCTMRLPPPTDKDAVTMEMIMATLPDVSQSCVQMAITWHLGRAQPDAIPLGQYPEQHFTERRALEMIDSFRKELKKIEEQILDQNAGLDLQYLF 594
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Medaka
Match: si:dkey-17e16.9 (hydroperoxide isomerase ALOXE3-like [Source:NCBI gene;Acc:101165367]) HSP 1 Score: 164.466 bits (415), Expect = 9.018e-42 Identity = 164/657 (24.96%), Postives = 288/657 (43.84%), Query Frame = 2
Query: 362 MMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDP---FSETRLEEIRHKQEIFSLYQRSSG--------FXXXXXXXXXXXKFSKSEKWDIDNAKLDAINQRRLTDLYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHL-EGWSVKHVIKARRLYIVDFKIMDGLNCKPDRI-------MPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAA-------RVTQSV-LGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQY 2248
++D WY KV + + E FP +WI G + K +E+ + R +E++ ++ SLYQ + G ++ +P E + S+ +I ++K + L G W S+E+L ++ + ++ D FG Q L G NP +K C+++P NF VT E +KP L G ++K ++ +++ D +IM+G+ P R+ M A + LF+ E +L PLAIQL Q ++ N +FLP+DP WLLAK++F AD +V L+ L ++ V R F HP+++LL PHF L ++ +EA+ DG + + +G+ +L+ R ++ + +++ +R L ++ N+ YRDDAL + + +FV +VK YP+ D+ D ELQ W E+ EN ++GV S + E++V +T ++ ++ V + + +D + + N SLQL P + ++ + ALP +V SV+ AA + T V LG F + +P ++++DF+ +L + +I N + + Y
Sbjct: 114 LLDDRWYCCKVEV------MTPEGEEIAFPCFRWISSGEEVELRGGKALKVFEEEHPLLIDHRNKELKLRK---SLYQWAVGDKKLPRHIHFTDISELPAEVQLSELRSDEIHHSKRKMNTELMFKGLAGSCKEWKSIEDLEKVFKSRKTSMSEYVSKHWKEDDFFGYQFLNGTNPNAIKRCSKLPPNFPVTEEMVKPCLPNGLTLKDEMEKGNIFLCDQRIMEGI---PTRVKDGNPLHMTAGLCLFYMNPEGKLMPLAIQLNQKASAENPIFLPTDPEKDWLLAKLFFKSADLLECEVVHHLLITHFLSEVFAVATLRCFPTIHPLHKLLIPHFRFTLHINIMGREALLGPDGALCAS-SFGLEGLMELMGRGLSETTYSALC-LPENITAR---GLDSIPNFYYRDDALKLWDVIESFVSSVVKDHYPSD-DDVFKDTELQEWIDEI-----------FTQGFLENKQSGVPAS-----------------------------FHTVEEVVKFVTMVI-FTVSVQHAAVNNGQFDFHSWIPNGSLQL-----------QKPPPTFKGQSRMNTFLQALP------NVGDSVKFAAMFWMLSDKYTDMVPLGAFPEERFSEPHLKQMIKDFQAELSYLSEEISLRNSKLQVPYAY 694
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Medaka
Match: alox12 (arachidonate 5-lipoxygenase [Source:NCBI gene;Acc:101174953]) HSP 1 Score: 164.851 bits (416), Expect = 9.519e-42 Identity = 153/665 (23.01%), Postives = 293/665 (44.06%), Query Frame = 2
Query: 323 KLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYD--CETFKGYEDP-FSETRLEEIRHKQEIFSLYQRSSGF-----XXXXXXXXXXXKFSKSEKWDIDNA---KLDAINQRRLTDLYGDQSFWSSLENLHNLYSQYNITEPIG--LTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHL-EGWSVKHVIKARRLYIVDFKIMDGL-----NCKPDRIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLI-NQMILENIYVCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQ-TSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPD-KHSTLDVLISVRMAARVTQSV-LGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQY 2248
+LE +W ++ +W+ V + G + FP ++W G + T K P E R +E++ ++E + ++G ++P++ +F ++ D +++ L ++ ++ T +G W+ LE+ ++ + + PI + D FG Q L G NP +++ C ++PDNF VT ++ + +G ++ +KA +Y++D+ IM+G+ KP + + AP+ L + + L P+AIQL Q+ +FLPSDPP++WLLAKMW A+ ++ L+ +++E V R HPIY+LLAPH L ++ + + + S+ G + + +G+ L +R YK ++ + D R V + NY YRD +L++ +A +FV MV L+YP +D+ D ELQ W R+++ G TQ + K+ +++ E+L +LL ++ ++ N +P+ P P + ++ ++ LPD S + + I+ + ++ LG++ + + AL++++ FRK+L+ I I N D+ + +
Sbjct: 137 RLEKQRYW------VEDNWFCRYVTVEPEDGG-----KMQIFPCYRWFVGDSKIEIREGKATTVKDDTSPKLLEHRKKELQERRETYRWITWAAGIPRCIDAKTEADLPQDARFENEKRSDFEHSLHYALLELSLKKFTIRFGKS--WNDLEDFKRIF--WKLRSPIAEYCMDHWKEDWLFGYQCLNGSNPRMIQRCQKLPDNFPVTAGMVQSSMADGTTLNKELKAGNIYLLDYAIMEGIPTNTIKGKP-QFIAAPLCLLYQHPDDGLIPIAIQLEQTPGLDTPIFLPSDPPLAWLLAKMWVRHAEFQVFQLLSHLLRTHLVVEVFCVATLRQLPAVHPIYKLLAPHQRYTLEINCRGRTQLLSETGIFKRVVSTGGEGLLVLAQREYKVLTYR-SLQPRFDFIDRGVTQIP---NYFYRDHSLMLWDAIHSFVTGMVDLYYPTD-QDVQKDAELQAWIRDVTQ-----------------------------------------EGFTQLPNFGLKSKLSSREELSTLLAVVIFTPTA------------QHAATNNGQFDWCAWVPNTPCTMRLPPPTDKDAVTMEMIMATLPDVSQSCVQMAITWHLGRAQPDAIPLGQYPEQHFTERRALEMIDSFRKELKKIEEQILDQNAGLDLQYLF 727
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Medaka
Match: alox5a (arachidonate 5-lipoxygenase [Source:NCBI gene;Acc:101160835]) HSP 1 Score: 157.532 bits (397), Expect = 1.229e-39 Identity = 147/668 (22.01%), Postives = 289/668 (43.26%), Query Frame = 2
Query: 317 FNKLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDPFS---ETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAINQRRLTD-------LYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLE-GWSVKHVIKARRLYIVDFKIMDGLNCKPD-----RIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRREL-SDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQS--VLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMD 2260
F KLE ++W + DWY + ++ +Y FP +W+ + D F +D S + R +E+ +++I+ + GFP+ + + + + ++D + +N + + ++ QS WS + ++ + T + D FG Q L GCNP++++ C ++PD F VT E + LE +++ ++A +YIVD +++DG++ + + AP+ L + +K++ P+AIQL Q E N +FLP+D WLLAK+W +D H V L+ ++ ++ + M R HP+Y+LL PH + ++ K +E + + G + G QL+++A K ++ + + ++SR + D++ + Y YRDD + A FV +V ++Y + + DEE+Q + +++ S MQD F ++ E+L+ LT ++ + + + YD + +P+ P P + N++ ++ +LPD+ + L +V ++ ++ LG + ++ + + +E FRKQL I I++ N + + + Y D
Sbjct: 67 FVKLEKKKYWVQD------DWYCRYITVKTPSG------DYLEFPCFRWLVDDSEVVLRDGRAFLPQDDKTSLVKQHRQKELETRRKIYKWKEWQPGFPMSINANKHK-ELPRDIQFDSEKGMDFLLNYSKAIENLCVNQFMHMFQSSWSDFGDFEKIFVRIKNTISEYVMEHWKEDFMFGYQFLNGCNPVLIRKCHKLPDKFPVTDEMVSVSLERELTLEQEMEAGNVYIVDCELLDGISANSTDPCTLQYLAAPICLLYKNNQKKIMPIAIQLGQDPKE-NPIFLPTDSEYDWLLAKIWVRSSDFQIHQTVTHLLRTHLMTEVFGIAMFRQLPAVHPVYKLLIPHIRFTIAINTKAREQLICECGIFDKANATGGGGHVQLIQKAMKTLTYK-SLCFPEMIKSRNMDDVEEMPTYFYRDDGYKVWEATKNFVSDVVSIYYTCDER-VQEDEEIQAFIKDVCSFGMQDF-----------------------------------------DHCEFPKSVKTCEELIEYLTVVV-FTASSQHAAVNFGQYDWCSW-----------IPNAPPTMRKPPPNEKGLANVNLIIDSLPDRGRSSWHLGAVWALSQFQENELYLGMYPDEHFIEKPVKQAMEKFRKQLTEISSFIKKRNDGKKLPYAYLSPD 665
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Medaka
Match: ENSORLT00000035939.1 (arachidonate 15-lipoxygenase B-like [Source:NCBI gene;Acc:101165945]) HSP 1 Score: 151.754 bits (382), Expect = 1.055e-37 Identity = 151/645 (23.41%), Postives = 279/645 (43.26%), Query Frame = 2
Query: 377 WYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDPFS---ETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXX-------KFSKSEKWDIDNAKLDAINQRRLTDLYGDQSFWSSLENLHNLY--SQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLEGWSVKHVIKARRLYIVDFKIMDG-----LNCKPDRIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIY-VCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGG--TQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYD---EYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQSV--LGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDI 2236
W+L V ++ Y + F FPVH+WI + F + + +D +R +E++ ++E + + + G P ++ F+KS ++ A + + D + W+++ ++ ++ Q +I + + D FG Q L G NP++++ CTE+P NF VT L P + S+ I+ +++ D+K +DG +N K +M AP+ L T +++L P+AIQL Q+ E N +FLP+D WLLAK++ AD H + L+ +L +Y V + RN HP+Y+LL PH L ++ + + S++G Q +G+ +++R+ ++ + I D+ R + D+ NY YRDD L + FV+ +++ +Y N +++ D E+Q W +SD +F G +Q S + +LV +T +IV GS + +YG +P+ P P + + N ++ LPD + T+ + +V + ++ + LG + + + K ++ F+ QLE + DI NK ++
Sbjct: 84 WFLSMVEVKS------PYGDTFTFPVHRWISDSNVHRFREAAALRVLDDKHHLGRYSREQELKVREEAYRWHVYTEGLPHCIKAEDLTSLPCEVQFSFTKSTEFAFTAASGLLELKLKGLD--NCKKGWTNIHDIDQVFCCKQTDIADYV--QKHWKEDSFFGIQYLNGVNPILIRRCTELPKNFPVTDSMLFPSGQ-MSLDAEIQKGNIFLCDYKHLDGVKTNTINGKTQYLM-APLVLLQKTADEKLMPIAIQLKQTPAEDNPIFLPTDSEYDWLLAKIFVRSADFSEHQLNTHLLRAHLLAEVYAVSLLRNVPMVHPLYKLLIPHTRYTLQINFLARNLLISENGSFTQFSASGGEGMLTILRRSLSAVTYS-SLCITDDIAERGMQDVP---NYFYRDDGLRLWYIIYRFVKGVIEYYYKND-QEVQQDTEIQKW---ISD---------------------------------------IFEHGFLSQASTGIPQSFTTLAELVKFVT-----MVIVTGSSQHAAVNSGQFDYG----------SWMPNTPISLKRPPPTKKGEANETTMLETLPDVNVTVQGMATVWLLSQQSSDFVPLGVYPEQHFTEETPRKFIKIFQAQLEELSNDIIARNKKLEV 654
BLAST of Arachidonate 5-lipoxygenase vs. Planmine SMEST
Match: SMESG000077360.1 (SMESG000077360.1) HSP 1 Score: 1463.74 bits (3788), Expect = 0.000e+0 Identity = 730/730 (100.00%), Postives = 730/730 (100.00%), Query Frame = 2
Query: 101 MGSNKSKIAFEICIVSGDYKEIEDPPSIFMVLFDEQQRQSPTLSYTNLIQDDEGKIQAKFSIENKPWCKIRNFNKLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDPFSETRLEEIRHKQEIFSLYQRSSGFXXXXXXXXXXXKFSKSEKWDIDNAKLDAINQRRLTDLYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLEGWSVKHVIKARRLYIVDFKIMDGLNCKPDRIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIYVCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDXXXXXXXXXXXENTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQSVLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMDPSILPNYPGV 2290
MGSNKSKIAFEICIVSGDYKEIEDPPSIFMVLFDEQQRQSPTLSYTNLIQDDEGKIQAKFSIENKPWCKIRNFNKLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDPFSETRLEEIRHKQEIFSLYQRSSGFPVEVENVPEEEKFSKSEKWDIDNAKLDAINQRRLTDLYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLEGWSVKHVIKARRLYIVDFKIMDGLNCKPDRIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIYVCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDGGMGIKGIPGGENTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQSVLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMDPSILPNYPGV
Sbjct: 1 MGSNKSKIAFEICIVSGDYKEIEDPPSIFMVLFDEQQRQSPTLSYTNLIQDDEGKIQAKFSIENKPWCKIRNFNKLEHIEFWRSNETMMDYDWYLEKVIIRDRRHGLRGYWEYFFFPVHQWIGGGRHYIFYDCETFKGYEDPFSETRLEEIRHKQEIFSLYQRSSGFPVEVENVPEEEKFSKSEKWDIDNAKLDAINQRRLTDLYGDQSFWSSLENLHNLYSQYNITEPIGLTNSIVNDICFGAQRLKGCNPLVLKLCTEIPDNFEVTVEWLKPHLEGWSVKHVIKARRLYIVDFKIMDGLNCKPDRIMPAPMALFFFTEEKQLKPLAIQLTQSSNEGNLVFLPSDPPVSWLLAKMWFNMADACHHMIVGRLINQMILENIYVCMRRNFGQSHPIYQLLAPHFTNCLVVSKKLKEAIFSKDGWVAQNIQLSKKGIKQLVKRAYKNWSFDVNANIHKDLESRKVYDLKALNNYPYRDDALLIHNAFTAFVRKMVKLFYPNGRKDLLLDEELQNWRRELSDPMQDGGMGIKGIPGGENTKNGVLKSTLDRDKAYADLNGSVFSGGTQTSVNFKNTINNPEDLVSLLTGILHLSIIVCGSGLRLPMYDEYGYPLNYSLQLLGNLPSDPTERTTDPLQIEQDQNLDQVVLALPDKHSTLDVLISVRMAARVTQSVLGKFDIDYIYQPEALKLLEDFRKQLEAIRLDIERSNKNRDIYHQYYCMDPSILPNYPGV 730 The following BLAST results are available for this feature:
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Arachidonate 5-lipoxygenase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Arachidonate 5-lipoxygenase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Arachidonate 5-lipoxygenase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Arachidonate 5-lipoxygenase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30032236 ID=SMED30032236|Name=Arachidonate 5-lipoxygenase|organism=Schmidtea mediterranea sexual|type=transcript|length=2684bpback to top protein sequence of SMED30032236-orf-1 >SMED30032236-orf-1 ID=SMED30032236-orf-1|Name=SMED30032236-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=731bp MGSNKSKIAFEICIVSGDYKEIEDPPSIFMVLFDEQQRQSPTLSYTNLIQback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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