Neuropeptide Y prohormone-10

Overview
NameNeuropeptide Y prohormone-10
Smed IDSMED30032184
Length (bp)267
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30032184

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30032184 SmedASXL_004238SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30032184 dd_Smed_v6_13645_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
gutSMED30032184 BK007011smed_ncbi_20240424PMID:20967238
Collins JJ et al., 2010		whole organism asexual adult colorimetric in situ hybridization evidence
cephalic gangliaSMED30032184 BK007011smed_ncbi_20240424PMID:20967238
Collins JJ et al., 2010		whole organism asexual adult colorimetric in situ hybridization evidence
ventral nerve cordSMED30032184 BK007011smed_ncbi_20240424PMID:20967238
Collins JJ et al., 2010		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of Neuropeptide Y prohormone-10 vs. TrEMBL
Match: E3CTI6 (Neuropeptide Y prohormone-10 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 1.804e-44
Identity = 71/71 (100.00%), Postives = 71/71 (100.00%), Query Frame = 1 
Query:    1 MNISRFLKVFVIFILFQNICTLSDYETSSFNQWYDKRDSKLLFNNAKQLLWYLQKLDKMYAIAGRPRYGKR 213
            MNISRFLKVFVIFILFQNICTLSDYETSSFNQWYDKRDSKLLFNNAKQLLWYLQKLDKMYAIAGRPRYGKR
Sbjct:    1 MNISRFLKVFVIFILFQNICTLSDYETSSFNQWYDKRDSKLLFNNAKQLLWYLQKLDKMYAIAGRPRYGKR 71
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BLAST of Neuropeptide Y prohormone-10 vs. Planmine SMEST
Match: SMESG000058298.1 (SMESG000058298.1)

HSP 1 Score: 147.132 bits (370), Expect = 5.017e-48
Identity = 71/71 (100.00%), Postives = 71/71 (100.00%), Query Frame = 1 
Query:    1 MNISRFLKVFVIFILFQNICTLSDYETSSFNQWYDKRDSKLLFNNAKQLLWYLQKLDKMYAIAGRPRYGKR 213
            MNISRFLKVFVIFILFQNICTLSDYETSSFNQWYDKRDSKLLFNNAKQLLWYLQKLDKMYAIAGRPRYGKR
Sbjct:    1 MNISRFLKVFVIFILFQNICTLSDYETSSFNQWYDKRDSKLLFNNAKQLLWYLQKLDKMYAIAGRPRYGKR 71
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BLAST of Neuropeptide Y prohormone-10 vs. Planmine SMEST
Match: SMESG000066803.1 (SMESG000066803.1)

HSP 1 Score: 40.4318 bits (93), Expect = 6.973e-6
Identity = 24/62 (38.71%), Postives = 36/62 (58.06%), Query Frame = 1 
Query:   28 FVIFILFQNICTLSDYETSSFNQWYDKRDSKLLFNNAKQLLWYLQKLDKMYAIAGRPRYGKR 213
            F++FIL    C +S    ++ NQ       + +F++A     YL+KL+  Y IAGRPR+GKR
Sbjct:    8 FLVFIL----CFMSFIHLNTCNQ------KRPMFDSADAFRNYLRKLNNEYMIAGRPRFGKR 59
The following BLAST results are available for this feature:
BLAST of Neuropeptide Y prohormone-10 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Neuropeptide Y prohormone-10 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Neuropeptide Y prohormone-10 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Neuropeptide Y prohormone-10 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Neuropeptide Y prohormone-10 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Neuropeptide Y prohormone-10 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Neuropeptide Y prohormone-10 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Neuropeptide Y prohormone-10 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
E3CTI61.804e-44100.00Neuropeptide Y prohormone-10 OS=Schmidtea mediterr... [more]
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BLAST of Neuropeptide Y prohormone-10 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Neuropeptide Y prohormone-10 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Neuropeptide Y prohormone-10 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Neuropeptide Y prohormone-10 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Neuropeptide Y prohormone-10 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Neuropeptide Y prohormone-10 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000058298.15.017e-48100.00SMESG000058298.1[more]
SMESG000066803.16.973e-638.71SMESG000066803.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30032184 ID=SMED30032184|Name=Neuropeptide Y prohormone-10|organism=Schmidtea mediterranea sexual|type=transcript|length=267bp
ATGAATATCAGTAGATTTCTGAAAGTATTTGTTATTTTTATTTTATTTCA
AAATATTTGCACGTTGAGTGATTATGAAACAAGTTCTTTCAATCAGTGGT
ACGACAAAAGAGATTCAAAACTATTATTCAATAATGCTAAACAATTGCTT
TGGTATTTGCAGAAATTGGACAAAATGTATGCTATAGCTGGAAGACCGAG
ATATGGTAAACGCTGATTTTCAGAACTGAATGTAATTTGAGATTAAAATA
AATATAATTAGTTGTTT
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000044cephalic ganglia
PLANA:0000097ventral nerve cord
PLANA:0000418head
Vocabulary: biological process
TermDefinition
GO:0007218neuropeptide signaling pathway
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..21
score: 0.756