SMED30032182

Overview
NameSMED30032182
Smed IDSMED30032182
Length (bp)1026
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30032182 (SMED30032182) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30032182 (SMED30032182) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30032182

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30032182h1SMcG0015731 SmedASXL_018751SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30032182h1SMcG0015731 SmedASXL_018751SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
gutSMED30032182h1SMcG0015731 dd_Smed_v4_975_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30032182h1SMcG0015731 dd_Smed_v6_975_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
head regionSMED30032182h1SMcG0015731 OX_Smed_1.0.20390ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30032182 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002690:33947..35021 +5032
Homology
BLAST of SMED30032182 vs. Ensembl Human
Match: KLK13 (kallikrein related peptidase 13 [Source:HGNC Symbol;Acc:HGNC:6361])

HSP 1 Score: 132.88 bits (333), Expect = 5.520e-36
Identity = 77/234 (32.91%), Postives = 126/234 (53.85%), Query Frame = -1
Query:  142 HQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYID-GNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 840
            H  P   ++ + G  +CGG L++   VLTAAHC+ +             +KV LG++ L  +   E+V +V   + HP Y+R+  +++  +D+ L++    +    T Y++ + L  + +++PG  C V GWG+      +  + LQ A + + S EEC + YP  +   + +CAG+      +C+GDSGGPL C    NR +  GIVS+   PCGQ ++P VYTR+S    WI
Sbjct:   45 HSQPWQAALLVQGRLLCGGVLVHPKWVLTAAHCLKE------------GLKVYLGKHALGRVEAGEQVREVVHSIPHPEYRRSPTHLNHDHDIMLLELQSPV--QLTGYIQTLPLSHNNRLTPGTTCRVSGWGTTTSPQVNYPKTLQCANIQLRSDEECRQVYPG-KITDNMLCAGTKEGGKDSCEGDSGGPLVC----NRTL-YGIVSWGDFPCGQPDRPGVYTRVSRYVLWI 258          
BLAST of SMED30032182 vs. Ensembl Human
Match: PRSS55 (serine protease 55 [Source:HGNC Symbol;Acc:HGNC:30824])

HSP 1 Score: 129.413 bits (324), Expect = 6.903e-34
Identity = 87/249 (34.94%), Postives = 126/249 (50.60%), Query Frame = -1
Query:  142 KIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPG----KRCEVVGWGSMQ-PDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCR-DPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 870
            +I  G    + ++P  VSIQ   +  CGG++LN   +LTAAHC++    +        ++ VVLG  DL   S   ++ +V  I++H  +KRA      ND+AL+     I  D+      +K+   L   PG    + C V GWG     D       L KA + I+  EECSK +P +    + +CAG  + S  ACKGDSGGPL C  +P  +  QVGI+S+ K  CG+ N P +YT +     WI
Sbjct:   67 RITGGMEAEVGEFPWQVSIQARSEPFCGGSILNKWWILTAAHCLYSEELFP------EELSVVLGTNDL--TSPSMEIKEVASIILHKDFKRANM---DNDIALLLLASPIKLDD------LKVPICLPTQPGPATWRECWVAGWGQTNAADKNSVKTDLMKAPMVIMDWEECSKMFPKLTK--NMLCAGYKNESYDACKGDSGGPLVCTPEPGEKWYQVGIISWGKS-CGEKNTPGIYTSLVNYNLWI 295          
BLAST of SMED30032182 vs. Ensembl Human
Match: PRSS55 (serine protease 55 [Source:HGNC Symbol;Acc:HGNC:30824])

HSP 1 Score: 129.028 bits (323), Expect = 7.654e-34
Identity = 87/249 (34.94%), Postives = 126/249 (50.60%), Query Frame = -1
Query:  142 KIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPG----KRCEVVGWGSMQ-PDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCR-DPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 870
            +I  G    + ++P  VSIQ   +  CGG++LN   +LTAAHC++    +        ++ VVLG  DL   S   ++ +V  I++H  +KRA      ND+AL+     I  D+      +K+   L   PG    + C V GWG     D       L KA + I+  EECSK +P +    + +CAG  + S  ACKGDSGGPL C  +P  +  QVGI+S+ K  CG+ N P +YT +     WI
Sbjct:   67 RITGGMEAEVGEFPWQVSIQARSEPFCGGSILNKWWILTAAHCLYSEELFP------EELSVVLGTNDL--TSPSMEIKEVASIILHKDFKRANM---DNDIALLLLASPIKLDD------LKVPICLPTQPGPATWRECWVAGWGQTNAADKNSVKTDLMKAPMVIMDWEECSKMFPKLTK--NMLCAGYKNESYDACKGDSGGPLVCTPEPGEKWYQVGIISWGKS-CGEKNTPGIYTSLVNYNLWI 295          
BLAST of SMED30032182 vs. Ensembl Human
Match: CTRL (chymotrypsin like [Source:HGNC Symbol;Acc:HGNC:2524])

HSP 1 Score: 121.709 bits (304), Expect = 6.784e-32
Identity = 83/249 (33.33%), Postives = 123/249 (49.40%), Query Frame = -1
Query:  142 KIINGSRTVMHQYPQIVSIQ-LDGDHICGGTLLNGNTVLTAAHC-VWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIK----LDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 870
            +I+NG   V+  +P  VS+Q   G H CGG+L++ + V+TAAHC V   R++           VVLGEYD    +   +VL V++ + HP++         NDV L+K          +Y   I       ++  ++ G  C   GWG +   G  T   LQ+  L +V+  +C + Y       S ICAG      S+C+GDSGGPL C+   N  + +GIVS+    C     P VYTR+S+ + WI
Sbjct:   33 RIVNGENAVLGSWPWQVSLQDSSGFHFCGGSLISQSWVVTAAHCNVSPGRHF-----------VVLGEYDRSSNAEPLQVLSVSRAITHPSWNSTTM---NNDVTLLKLA-----SPAQYTTRISPVCLASSNEALTEGLTCVTTGWGRLSGVGNVTPAHLQQVALPLVTVNQC-RQYWGSSITDSMICAGGA--GASSCQGDSGGPLVCQK-GNTWVLIGIVSWGTKNC-NVRAPAVYTRVSKFSTWI 257          
BLAST of SMED30032182 vs. Ensembl Human
Match: GZMM (granzyme M [Source:HGNC Symbol;Acc:HGNC:4712])

HSP 1 Score: 119.398 bits (298), Expect = 4.171e-31
Identity = 77/250 (30.80%), Postives = 123/250 (49.20%), Query Frame = -1
Query:  142 SASGWKIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLK-VSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKT-YPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 885
            S+ G +II G   + H  P + S+Q +G H+CGG L++   VLTAAHC+              ++++VLG   LH +        +   + HP YK   A    ND+AL++ DG +    T  ++ + L +  + V+ G RC + GWG +   G   S+ L++  L ++ T  C+ + + N    PS +C  + S   + CKGDSGGPL C       +   ++S+S   C    KP V T ++    WI
Sbjct:   20 SSFGTQIIGGREVIPHSRPYMASLQRNGSHLCGGVLVHPKWVLTAAHCLAQ---------RMAQLRLVLG---LHTLDSPGLTFHIKAAIQHPRYKPVPAL--ENDLALLQLDGKVKPSRT--IRPLALPSKRQVVAAGTRCSMAGWG-LTHQGGRLSRVLRELDLQVLDTRMCNNSRFWNGSLSPSMVCLAADSKDQAPCKGDSGGPLVC---GKGRVLARVLSFSSRVCTDIFKPPVATAVAPYVSWI 249          
BLAST of SMED30032182 vs. Ensembl Celegans
Match: svh-1 (HGF/MSP/plasminogen-like protein [Source:UniProtKB/TrEMBL;Acc:Q17800])

HSP 1 Score: 108.997 bits (271), Expect = 7.613e-26
Identity = 71/250 (28.40%), Postives = 127/250 (50.80%), Query Frame = -1
Query:  139 KIINGSRTVMHQYPQIVSIQLDGD--HICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDT-DLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEEC---SKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWIT 870
            +++ G  TV   +P   +++      H CG ++L+   ++TAAHC  +    +         +VV+G++D +   G E++  + +I  +P YK     I  +D+A+++   +   +  EY + I L + D   +PG++C V GWGSM   G   ++ LQ A + I++  +C   S+ Y ++    S  CAG     I +C+GDSGGP  CR      +  G++S+    C Q  +P +YT ++    WI+
Sbjct:  712 RVVGGFETVPGAFPWTAALRNKATKAHHCGASILDKTHLITAAHCFEEDERVS-------SYEVVVGDWDNNQTDGNEQIFYLQRIHFYPLYKD----IFSHDIAILEIP-YPGIEFNEYAQPICLPSKDFVYTPGRQCVVSGWGSM---GLRYAERLQAALIPIINRFDCVNSSQIYSSMSR--SAFCAGYLEGGIDSCQGDSGGPFACRREDGAFVLAGVISWGDG-CAQKKQPGIYTMVAPYLSWIS 943          
BLAST of SMED30032182 vs. Ensembl Celegans
Match: try-1 (TRYpsin-like protease [Source:UniProtKB/TrEMBL;Acc:Q23528])

HSP 1 Score: 96.2857 bits (238), Expect = 1.106e-22
Identity = 78/249 (31.33%), Postives = 116/249 (46.59%), Query Frame = -1
Query:  136 KIINGSRTVMHQYPQIVSI-QLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSK--TYPNVQNVPSNICAGSGSLSISACKGDSGGPLFC-RDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWITL 870
            ++I GS +  H +P  V +    G H CGG+L++ N VLTAAHC    R  T          V +G     H SG     +V  + +HP Y     +    D A+M+    ++   T   + I L + L     + C V GWGS       ++  L++  + ++ST  CS    Y    ++PS +CAG     I +C+GDSGGPL C RD    L   G+VS+  + C +   P VY  +   + WI L
Sbjct:   57 RLIGGSESSPHSWPWTVQLLSRLGHHRCGGSLIDPNFVLTAAHCFAKDRRPT-------SYSVRVGG----HRSGSGSPHRVTAVSIHPWYN--IGFPSSYDFAIMRIHPPVNTSTT--ARPICLPS-LPAVENRLCVVTGWGSTIEGSSLSAPTLREIHVPLLSTLFCSSLPNYIGRIHLPSMLCAGYSYGKIDSCQGDSGGPLMCARDGHWEL--TGVVSWG-IGCARPGMPGVYGNVHSASTWINL 286          
BLAST of SMED30032182 vs. Ensembl Celegans
Match: try-3 (TRYpsin-like protease [Source:UniProtKB/TrEMBL;Acc:Q94176])

HSP 1 Score: 71.633 bits (174), Expect = 3.715e-14
Identity = 65/235 (27.66%), Postives = 102/235 (43.40%), Query Frame = -1
Query:  142 ICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTS--------QFLQKAFLDIVSTEECSKTYP-----NVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSK----MPCGQANKPTVYTRISEVAKWI 795
            +CG T+++   ++TAAHC   ++  +  +    K             + +E+   V +  +H  Y    A    ND+AL++    +S    + V  +  D+ L +   K   V+G+G     GED+S        Q LQ   + I+S ++C KT+      +V+     ICAG+         GDSGGPL         +Q+GI SY          Q   P VYTRIS+   WI
Sbjct:   15 LCGATVIDDFWLVTAAHCALQLQTRSFVYVREPK-------------NNRERSFSVKEAYIHSGYNNQTA---DNDIALLRISSDLSKLGIKPVCLVHDDSKL-LKQYKNGVVIGYGLTL--GEDSSGEPKLINSQTLQSTSVPIISDDDCVKTWRFLSLLSVKITGYQICAGA--YLHGTAPGDSGGPLLIHKSNGEYVQIGITSYGADGLDGVIDQGKFPGVYTRISKYVPWI 228          
BLAST of SMED30032182 vs. Ensembl Celegans
Match: try-3 (TRYpsin-like protease [Source:UniProtKB/TrEMBL;Acc:Q94176])

HSP 1 Score: 72.0182 bits (175), Expect = 4.749e-14
Identity = 65/235 (27.66%), Postives = 102/235 (43.40%), Query Frame = -1
Query:  142 ICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTS--------QFLQKAFLDIVSTEECSKTYP-----NVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSK----MPCGQANKPTVYTRISEVAKWI 795
            +CG T+++   ++TAAHC   ++  +  +    K             + +E+   V +  +H  Y    A    ND+AL++    +S    + V  +  D+ L +   K   V+G+G     GED+S        Q LQ   + I+S ++C KT+      +V+     ICAG+         GDSGGPL         +Q+GI SY          Q   P VYTRIS+   WI
Sbjct:   65 LCGATVIDDFWLVTAAHCALQLQTRSFVYVREPK-------------NNRERSFSVKEAYIHSGYNNQTA---DNDIALLRISSDLSKLGIKPVCLVHDDSKL-LKQYKNGVVIGYGLTL--GEDSSGEPKLINSQTLQSTSVPIISDDDCVKTWRFLSLLSVKITGYQICAGA--YLHGTAPGDSGGPLLIHKSNGEYVQIGITSYGADGLDGVIDQGKFPGVYTRISKYVPWI 278          
BLAST of SMED30032182 vs. Ensembl Fly
Match: CG32808 (gene:FBgn0052808 transcript:FBtr0070295)

HSP 1 Score: 122.479 bits (306), Expect = 2.440e-32
Identity = 95/270 (35.19%), Postives = 134/270 (49.63%), Query Frame = -1
Query:  130 FYIVTFLVLTYNLSASGWKIINGSRTVMHQYPQIVSIQL--DGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVV-GWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI--TLNS 924
            FY  TFL+     S    KI+NG+     ++P +VS++    G H CG TLLN   VLTAAHCV       +D  +Y       G   L   S   +V +V  I +HP Y+    Y+  ND+AL++    ++   +++V+ ++L    +V+PG    V+ GWG +   G    Q LQK  L + S  ECS+ +    +  S ICAG        C GDSGGPL          QVGIVS+S  PC +   P V+T +S    WI  T+NS
Sbjct:   13 FYTATFLLA--GASGEDGKIVNGTTAGPGEFPFVVSLRRAKSGRHSCGATLLNPYWVLTAAHCVRGSSPEQLD-LQY-------GSQMLARNS--SQVARVAAIFVHPGYEPEDKYV--NDIALLQLAQSVA--LSKFVQPVRLPEPRQVTPGNASAVLAGWG-LNATGGVVQQHLQKVKLQVFSDTECSERHQTYLH-DSQICAGLPEGGKGQCSGDSGGPLLL---IGSDTQVGIVSWSIKPCARPPFPGVFTEVSAYVDWIVETVNS 261          
BLAST of SMED30032182 vs. Ensembl Fly
Match: CG11911 (gene:FBgn0031249 transcript:FBtr0078098)

HSP 1 Score: 114.39 bits (285), Expect = 2.422e-29
Identity = 84/256 (32.81%), Postives = 132/256 (51.56%), Query Frame = -1
Query:  139 ASGWKIINGSRTVMHQYPQIVSIQ---LDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKF-DGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVP-SNICAGSGSLSISACKGDSGGPL---FCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWIT 882
            A+G+ +ING+    H  P IVS+    L   HICGGTL+N + ++TAAHC+           E   + ++ G   LH  +  +++ Q  ++     +++    +   D+AL+   + FI N   E+V+   L +  +V  G+   + GWG  +      ++ LQ     I++ EEC +  P    +  SNIC+ S   S SAC GDSGGPL   F   P+     +GIVS+  +PCG AN P++YT++S    WIT
Sbjct:   33 ATGF-VINGTEAEPHSAPYIVSLATNYLKHSHICGGTLINKDWIVTAAHCI----------SEPVGMSIIAG---LHTRAEVDELTQQRQVDFGRVHEKYTGGVGPYDIALLHVNESFIFN---EWVQPATLPSREQVHEGET-HLYGWGQPKSYIFSGAKTLQTVTTQILNYEECKEELPESAPIAESNICSSSLQQSKSACNGDSGGPLVVEFTNAPSEL---IGIVSWGYIPCGLANMPSIYTKVSAYIDWIT 267          
BLAST of SMED30032182 vs. Ensembl Fly
Match: CG11836 (gene:FBgn0039272 transcript:FBtr0309271)

HSP 1 Score: 110.153 bits (274), Expect = 9.637e-28
Identity = 83/249 (33.33%), Postives = 131/249 (52.61%), Query Frame = -1
Query:  133 KIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQ--VNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEEC-SKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWITLN 870
            +I+ G  T ++QYP +  I  DG   CGG+LL  + VL+AAHCV  +R          K++V+ G++D   I+ + + +Q  V  ++ H ++     Y   ND+AL++    IS   ++ +K I L        G+   VVGWG     GE  S  + +  + I+S  EC ++ Y + +   S +CAG  S+   +C+GDSGGPL   +     I VGIVS+  + CG+   P VY+R+S+   WI  N
Sbjct:   44 RIVGGKPTGVNQYPWMARIVYDGKFHCGGSLLTKDYVLSAAHCVKKLR--------KSKIRVIFGDHD-QEITSESQAIQRAVTAVIKHKSFD-PDTY--NNDIALLRLRKPISF--SKIIKPICLPRYNYDPAGRIGTVVGWGRTSEGGELPS-IVNQVKVPIMSITECRNQRYKSTRITSSMLCAGRPSM--DSCQGDSGGPLLLSNGVKYFI-VGIVSWG-VGCGREGYPGVYSRVSKFIPWIKSN 273          
BLAST of SMED30032182 vs. Ensembl Fly
Match: CG11836 (gene:FBgn0039272 transcript:FBtr0473579)

HSP 1 Score: 110.923 bits (276), Expect = 1.622e-27
Identity = 83/249 (33.33%), Postives = 131/249 (52.61%), Query Frame = -1
Query:  133 KIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQ--VNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEEC-SKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWITLN 870
            +I+ G  T ++QYP +  I  DG   CGG+LL  + VL+AAHCV  +R          K++V+ G++D   I+ + + +Q  V  ++ H ++     Y   ND+AL++    IS   ++ +K I L        G+   VVGWG     GE  S  + +  + I+S  EC ++ Y + +   S +CAG  S+   +C+GDSGGPL   +     I VGIVS+  + CG+   P VY+R+S+   WI  N
Sbjct:   96 RIVGGKPTGVNQYPWMARIVYDGKFHCGGSLLTKDYVLSAAHCVKKLR--------KSKIRVIFGDHD-QEITSESQAIQRAVTAVIKHKSFD-PDTY--NNDIALLRLRKPIS--FSKIIKPICLPRYNYDPAGRIGTVVGWGRTSEGGELPS-IVNQVKVPIMSITECRNQRYKSTRITSSMLCAGRPSM--DSCQGDSGGPLLLSNGVKYFI-VGIVSWG-VGCGREGYPGVYSRVSKFIPWIKSN 325          
BLAST of SMED30032182 vs. Ensembl Fly
Match: CG7432 (gene:FBgn0038727 transcript:FBtr0301213)

HSP 1 Score: 114.005 bits (284), Expect = 1.655e-27
Identity = 86/259 (33.20%), Postives = 131/259 (50.58%), Query Frame = -1
Query:  142 SGWKIINGSRTVMHQYPQIVSIQLDG----DHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEK--VLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSP-----GKRCEVVGWGSMQPDG-EDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSN-ICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 879
            S  +I+ G      Q+P + +I L G    +  CGG+L+    +LTAAHC  D R      F   +  V LG+ DL   +         V ++  H  + R   Y   ND+A++  D  +   +++YV  + L   +++ P     G+R  VVGWG+    G E TSQ  ++A L I   E+C ++Y   Q +  N ICAG     + AC+GDSGGPL  R  ++  +Q+G+VS+    CG+   P VYTR++E   WI
Sbjct:  471 STGRIVGGVEAPNGQWPWMAAIFLHGPKRTEFWCGGSLIGTKYILTAAHCTRDSRQKP---FAARQFTVRLGDIDLSTDAEPSDPVTFAVKEVRTHERFSRIGFY---NDIAILVLDKPVR--KSKYVIPVCLPKGIRMPPKERLPGRRATVVGWGTTYYGGKESTSQ--RQAELPIWRNEDCDRSY--FQPINENFICAGYSDGGVDACQGDSGGPLMMRYDSH-WVQLGVVSFGNK-CGEPGYPGVYTRVTEYLDWI 715          
BLAST of SMED30032182 vs. Ensembl Zebrafish
Match: cela1.6 (chymotrypsin like elastase family member 1, tandem duplicate 6 [Source:ZFIN;Acc:ZDB-GENE-040808-55])

HSP 1 Score: 125.176 bits (313), Expect = 2.734e-33
Identity = 87/252 (34.52%), Postives = 132/252 (52.38%), Query Frame = -1
Query:  142 KIINGSRTVMHQYPQIVSIQ-LDGD---HICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSP-GKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKT---YPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSY-SKMPCGQANKPTVYTRISEVAKWI 870
            +++ G     + +P  +S+Q L G    H CGGTL+  N VLTAAHCV   R +          +VVLGE+D++   G+E+ + V+ + +HP + R      G D+AL++     S +   YV+   L    +V P    C + GWG     G  ++Q L++A+L +V    CS+       V+N  + +CAG GSLS   C+GDSGGPL C+     ++  G+ S+ S   C    KPTV+TR+S    WI
Sbjct:   29 RVVGGEVARPNSWPWQISLQYLSGGSYYHTCGGTLIKQNFVLTAAHCVDTSRTW----------RVVLGEHDIYKQEGREQYMTVSNVYIHPNWNRNNV-AAGYDIALLRLSSNASLNT--YVQLGTLPPSGQVLPHNNACYITGWGLTSTGGSLSAQ-LKQAYLPVVDYNTCSRGDWWGSTVKN--TMVCAGGGSLS--GCQGDSGGPLNCQVSGQYVVH-GVTSFVSSSGCNAYQKPTVFTRVSAYISWI 261          
BLAST of SMED30032182 vs. Ensembl Zebrafish
Match: cela1.6 (chymotrypsin like elastase family member 1, tandem duplicate 6 [Source:ZFIN;Acc:ZDB-GENE-040808-55])

HSP 1 Score: 125.176 bits (313), Expect = 2.917e-33
Identity = 87/252 (34.52%), Postives = 132/252 (52.38%), Query Frame = -1
Query:  142 KIINGSRTVMHQYPQIVSIQ-LDGD---HICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSP-GKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKT---YPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSY-SKMPCGQANKPTVYTRISEVAKWI 870
            +++ G     + +P  +S+Q L G    H CGGTL+  N VLTAAHCV   R +          +VVLGE+D++   G+E+ + V+ + +HP + R      G D+AL++     S +   YV+   L    +V P    C + GWG     G  ++Q L++A+L +V    CS+       V+N  + +CAG GSLS   C+GDSGGPL C+     ++  G+ S+ S   C    KPTV+TR+S    WI
Sbjct:   29 RVVGGEVARPNSWPWQISLQYLSGGSYYHTCGGTLIKQNFVLTAAHCVDTSRTW----------RVVLGEHDIYKQEGREQYMTVSNVYIHPNWNRNNV-AAGYDIALLRLSSNASLNT--YVQLGTLPPSGQVLPHNNACYITGWGLTSTGGSLSAQ-LKQAYLPVVDYNTCSRGDWWGSTVKN--TMVCAGGGSLS--GCQGDSGGPLNCQVSGQYVVH-GVTSFVSSSGCNAYQKPTVFTRVSAYISWI 261          
BLAST of SMED30032182 vs. Ensembl Zebrafish
Match: tmprss15 (transmembrane serine protease 15 [Source:ZFIN;Acc:ZDB-GENE-091204-83])

HSP 1 Score: 125.946 bits (315), Expect = 2.375e-31
Identity = 77/251 (30.68%), Postives = 127/251 (50.60%), Query Frame = -1
Query:  127 KIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEY-DLHHISGKEKVLQVNKIMMHPTY-KRAKAYIDGNDVALMKFDGFISNDETEYVKYIKL-DTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWITLNSK 870
            +++ G       +P +VS+Q  G H CG TL++   ++TAAHCV+       +W        VLG +     I+  ++V  V++++MH  Y KR K     +D ALM     +S   T+YV+ I L D       G++C + GWG +   G+  S  LQ+A + ++S  +C +  P        +CAG     +  C+GDSGGPL C +  + ++ VG  S+  + CG+  +P  Y R+S+   W+  N +
Sbjct:  642 RVVGGQDAQRGAWPWMVSLQWLGGHACGATLIDREWLITAAHCVYGRNVQLSNW------AAVLGLHAQFETINPNKQVFSVDQVIMHKHYNKRTKE----SDFALMHLKTPVS--YTDYVQPICLPDPGAHFEEGRKCFIAGWGLLSESGQ-ISDVLQQAVVPLLSNTQCQEWLPEYNFTERMMCAGYAEGGVDTCQGDSGGPLMCEEEGHWVL-VGATSFG-IGCGRPQRPGAYARVSQFVDWVAENRR 877          
BLAST of SMED30032182 vs. Ensembl Zebrafish
Match: cela1.5 (chymotrypsin like elastase family member 1, tandem duplicate 5 [Source:ZFIN;Acc:ZDB-GENE-040801-186])

HSP 1 Score: 119.013 bits (297), Expect = 6.827e-31
Identity = 82/255 (32.16%), Postives = 128/255 (50.20%), Query Frame = -1
Query:  133 KIINGSRTVMHQYPQIVSIQLDG------DHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSP-GKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSN-ICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSY-SKMPCGQANKPTVYTRISEVAKWITLN 870
            +++ G     H +P  VS+Q+         H C GTL+  N VLT+ H ++          +Y   +VVLG++D+    G E+ ++V +I  H  Y        GNDVAL+K       +   YV+   L    ++ P G  C   GWG+ + DG  +++ L++A+L +V  E CS++      V    +C G G++++  CKGD GGPL C     + +  GI S+ S   C    KPTV+TR+S    WIT+N
Sbjct:   37 RVVGGEIAKPHSWPWQVSVQIKTLSQDSYTHYCAGTLIRKNYVLTSVHSIFS---------KYGSWRVVLGDHDISTDEGTEQYIEVREITFH-AYSDLNDVSKGNDVALLKLAS--DANLNAYVQLAPLPRHKQILPHGTPCFTTGWGNTETDGSFSAE-LKQAYLPVVDHETCSQSDWWGSTVKDTMVCGGDGTMAV--CKGDFGGPLSCL-VDGKYVVYGIASFMSSEGCNIYKKPTVFTRVSAYVDWITIN 275          
BLAST of SMED30032182 vs. Ensembl Zebrafish
Match: cela1.5 (chymotrypsin like elastase family member 1, tandem duplicate 5 [Source:ZFIN;Acc:ZDB-GENE-040801-186])

HSP 1 Score: 118.627 bits (296), Expect = 8.677e-31
Identity = 81/255 (31.76%), Postives = 128/255 (50.20%), Query Frame = -1
Query:  133 KIINGSRTVMHQYPQIVSIQLDG------DHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSP-GKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSN-ICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSY-SKMPCGQANKPTVYTRISEVAKWITLN 870
            +++ G     H +P  VS+Q+         H C GTL+  N VLT+ H ++          +Y   +VVLG++D+    G E+ ++V +I  H  Y        GNDVAL+K       +   YV+   L    ++ P G  C   GWG+ + DG  +++ L++A+L +V  E CS++      V    +C G G++++  CKGD GGPL C     + +  GI S+ S   C    KPT++TR+S    WIT+N
Sbjct:   29 RVVGGEIAKPHSWPWQVSVQIKTLSQDSYTHYCAGTLIRKNYVLTSVHSIFS---------KYGSWRVVLGDHDISTDEGTEQYIEVREITFH-AYSDLNDVSKGNDVALLKLAS--DANLNAYVQLAPLPRHKQILPHGTPCFTTGWGNTETDGSFSAE-LKQAYLPVVDHETCSQSDWWGSTVKDTMVCGGDGTMAV--CKGDFGGPLSCL-VDGKYVVYGIASFMSSEGCNIYKKPTIFTRVSAYVDWITIN 267          
BLAST of SMED30032182 vs. Ensembl Xenopus
Match: il11ra (interleukin 11 receptor subunit alpha [Source:Xenbase;Acc:XB-GENE-483094])

HSP 1 Score: 133.265 bits (334), Expect = 2.302e-34
Identity = 85/258 (32.95%), Postives = 136/258 (52.71%), Query Frame = -1
Query:  139 FLVLTYNLSASGWKIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDP-ANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWIT 909
            F  ++ N    G +I+ GS T    +P +V+I+ +G+ +CGG LL+   +LTAAHC        + W       VV+G+YDL   +  EK  QVN+I+ HP + + K +   ND+AL++    ++  ++     +        +PG  C + GWGS+  DG   S  + +A + ++S E C  T        +  CAG  +  I +C+GDSGGPL C+DP + + +  GI S+    CG+  KP VYTR++    WI+
Sbjct:   62 FSSISNNTGPKG-RIVGGSITSPGSWPWLVNIRFNGELMCGGVLLDDMWILTAAHCFTGSVNEVL-W------TVVVGQYDLTKNAQGEKTFQVNRIVTHPKFNQ-KTF--DNDLALLELTSSVTASQSARPVCLP-PVPRDPTPGTNCYIAGWGSLYEDGP-LSDVIMEARVPVLSQEACRSTLGKNMLTSTMFCAGYLNGGIDSCQGDSGGPLTCQDPISKQYVLYGITSWGDG-CGERGKPGVYTRVTAFTDWIS 305          
BLAST of SMED30032182 vs. Ensembl Xenopus
Match: il11ra (interleukin 11 receptor subunit alpha [Source:Xenbase;Acc:XB-GENE-483094])

HSP 1 Score: 132.88 bits (333), Expect = 8.555e-34
Identity = 85/258 (32.95%), Postives = 136/258 (52.71%), Query Frame = -1
Query:  139 FLVLTYNLSASGWKIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDP-ANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWIT 909
            F  ++ N    G +I+ GS T    +P +V+I+ +G+ +CGG LL+   +LTAAHC        + W       VV+G+YDL   +  EK  QVN+I+ HP + + K +   ND+AL++    ++  ++     +        +PG  C + GWGS+  DG   S  + +A + ++S E C  T        +  CAG  +  I +C+GDSGGPL C+DP + + +  GI S+    CG+  KP VYTR++    WI+
Sbjct:  183 FSSISNNTGPKG-RIVGGSITSPGSWPWLVNIRFNGELMCGGVLLDDMWILTAAHCFTGSVNEVL-W------TVVVGQYDLTKNAQGEKTFQVNRIVTHPKFNQ-KTF--DNDLALLELTSSVTASQSARPVCLP-PVPRDPTPGTNCYIAGWGSLYEDGP-LSDVIMEARVPVLSQEACRSTLGKNMLTSTMFCAGYLNGGIDSCQGDSGGPLTCQDPISKQYVLYGITSWGDG-CGERGKPGVYTRVTAFTDWIS 426          
BLAST of SMED30032182 vs. Ensembl Xenopus
Match: ENSXETT00000030351.1 (transmembrane serine protease 11F [Source:NCBI gene;Acc:100379970])

HSP 1 Score: 124.405 bits (311), Expect = 3.635e-33
Identity = 86/250 (34.40%), Postives = 136/250 (54.40%), Query Frame = -1
Query:  130 KIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSP-GKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKT--YPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWITLNS 870
            +I+ G+   +  +P   S++L G H CG +LLN   ++ AAHC +DM      W       VVLG  +++  SG E   ++ KI+++  Y    ++   ND+AL+K   F   + T  ++ + L     + P G  C + GWG++  DG   SQ LQ+A + I++++ CS +  Y  +   PS ICAG  +  I +C+GDSGGPL     + R + +GIVS+    C   NKP VY+RI+ +  WIT +S
Sbjct:   16 RIVGGTNAGLGSWPWQASLRLLGSHTCGASLLNDTWLVAAAHC-FDMNADANSW------TVVLGTINVY--SGSE--FKIEKIIIYEGY---TSHNHRNDIALLKL--FTPLNFTSIIRPVCLPEASDIFPDGSSCYITGWGALT-DGGSASQVLQQAEVKIINSDTCSSSQMYGGLI-YPSMICAGYATGQIDSCQGDSGGPLVTLK-SGRWVLIGIVSFG-YGCALPNKPGVYSRITYLRNWITAHS 245          
BLAST of SMED30032182 vs. Ensembl Xenopus
Match: ENSXETT00000014775.1 (transmembrane serine protease 11F [Source:NCBI gene;Acc:100379970])

HSP 1 Score: 124.405 bits (311), Expect = 3.763e-33
Identity = 86/250 (34.40%), Postives = 136/250 (54.40%), Query Frame = -1
Query:  130 KIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSP-GKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKT--YPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWITLNS 870
            +I+ G+   +  +P   S++L G H CG +LLN   ++ AAHC +DM      W       VVLG  +++  SG E   ++ KI+++  Y    ++   ND+AL+K   F   + T  ++ + L     + P G  C + GWG++  DG   SQ LQ+A + I++++ CS +  Y  +   PS ICAG  +  I +C+GDSGGPL     + R + +GIVS+    C   NKP VY+RI+ +  WIT +S
Sbjct:   14 RIVGGTNAGLGSWPWQASLRLLGSHTCGASLLNDTWLVAAAHC-FDMNADANSW------TVVLGTINVY--SGSE--FKIEKIIIYEGY---TSHNHRNDIALLKL--FTPLNFTSIIRPVCLPEASDIFPDGSSCYITGWGALT-DGGSASQVLQQAEVKIINSDTCSSSQMYGGLI-YPSMICAGYATGQIDSCQGDSGGPLVTLK-SGRWVLIGIVSFG-YGCALPNKPGVYSRITYLRNWITAHS 243          
BLAST of SMED30032182 vs. Ensembl Xenopus
Match: ENSXETT00000030355.1 (transmembrane serine protease 11F [Source:NCBI gene;Acc:100379970])

HSP 1 Score: 124.405 bits (311), Expect = 3.857e-33
Identity = 86/250 (34.40%), Postives = 136/250 (54.40%), Query Frame = -1
Query:  130 KIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSP-GKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKT--YPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWITLNS 870
            +I+ G+   +  +P   S++L G H CG +LLN   ++ AAHC +DM      W       VVLG  +++  SG E   ++ KI+++  Y    ++   ND+AL+K   F   + T  ++ + L     + P G  C + GWG++  DG   SQ LQ+A + I++++ CS +  Y  +   PS ICAG  +  I +C+GDSGGPL     + R + +GIVS+    C   NKP VY+RI+ +  WIT +S
Sbjct:   15 RIVGGTNAGLGSWPWQASLRLLGSHTCGASLLNDTWLVAAAHC-FDMNADANSW------TVVLGTINVY--SGSE--FKIEKIIIYEGY---TSHNHRNDIALLKL--FTPLNFTSIIRPVCLPEASDIFPDGSSCYITGWGALT-DGGSASQVLQQAEVKIINSDTCSSSQMYGGLI-YPSMICAGYATGQIDSCQGDSGGPLVTLK-SGRWVLIGIVSFG-YGCALPNKPGVYSRITYLRNWITAHS 244          
BLAST of SMED30032182 vs. Ensembl Mouse
Match: Klk13 (kallikrein related-peptidase 13 [Source:MGI Symbol;Acc:MGI:3615275])

HSP 1 Score: 125.176 bits (313), Expect = 3.249e-33
Identity = 82/267 (30.71%), Postives = 134/267 (50.19%), Query Frame = -1
Query:  142 LVLTYNLSASGWKIINGSR-----------TVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYID-GNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 906
            L L+  +S    KI+NG+             + H  P   ++ + G  +CGG L++   VLTAAHC  D   YT          V LG++ L  +   E+ ++V + + HP Y+    +++  +D+ L++    +    + +V+ +KL  D  +  G  C V GWG+      +  + LQ A +++ S EEC + YP  +   + +CAG+      +C+GDSGGPL C          GI+S+   PCGQ N+P VYTR+S+  +WI
Sbjct:   13 LALSEGISRDYPKILNGTNGTSGFLPGGYTCLPHSQPWQAALLIRGRLLCGGVLVHPKWVLTAAHCRKDG--YT----------VHLGKHALGRVENGEQAMEVVRSIPHPEYQVTPTHLNHDHDIMLLELKSPV--QLSSHVRTLKLSADDCLPTGTCCRVSGWGTTTSPQVNYPKTLQCANIELRSDEECRQVYPG-KITANMLCAGTKEGGKDSCEGDSGGPLICNGKL-----YGIISWGDFPCGQPNRPGVYTRVSKYLRWI 259          
BLAST of SMED30032182 vs. Ensembl Mouse
Match: Tmprss3 (transmembrane protease, serine 3 [Source:MGI Symbol;Acc:MGI:2155445])

HSP 1 Score: 125.176 bits (313), Expect = 8.054e-32
Identity = 91/264 (34.47%), Postives = 140/264 (53.03%), Query Frame = -1
Query:  142 VLTYNLSASGWK------IINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSP-GKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSK--TYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQ-VGIVSYSKMPCGQANKPTVYTRISEVAKWI 903
            V+T   SA G +      I+ G+ + + Q+P  VS+Q  G H+CGG+++    ++TAAHCV+D+ Y+   W     +  ++      H+        V KI+ H  YK  +    GND+ALMK    ++ DET  ++ I L    +  P GK C   GWG+ + DG D S  L  A + ++S + C+    Y  + + PS +CAG     + +C+GDSGGPL C++   RL + VG  S+  + C + NKP VYTRI+    WI
Sbjct:  221 VVTLKCSACGTRTGYSPRIVGGNMSSLTQWPWQVSLQFQGYHLCGGSIITPLWIVTAAHCVYDL-YHPKSWTVQVGLVSLMDSPVPSHL--------VEKIIYHSKYKPKRL---GNDIALMKLSEPLTFDET--IQPICLPNSEENFPDGKLCWTSGWGATE-DGGDASPVLNHAAVPLISNKICNHRDVYGGIIS-PSMLCAGYLKGGVDSCQGDSGGPLVCQE--RRLWKLVGATSFG-IGCAEVNKPGVYTRITSFLDWI 465          
BLAST of SMED30032182 vs. Ensembl Mouse
Match: Tmprss3 (transmembrane protease, serine 3 [Source:MGI Symbol;Acc:MGI:2155445])

HSP 1 Score: 124.79 bits (312), Expect = 8.558e-32
Identity = 91/264 (34.47%), Postives = 140/264 (53.03%), Query Frame = -1
Query:  142 VLTYNLSASGWK------IINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSP-GKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSK--TYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQ-VGIVSYSKMPCGQANKPTVYTRISEVAKWI 903
            V+T   SA G +      I+ G+ + + Q+P  VS+Q  G H+CGG+++    ++TAAHCV+D+ Y+   W     +  ++      H+        V KI+ H  YK  +    GND+ALMK    ++ DET  ++ I L    +  P GK C   GWG+ + DG D S  L  A + ++S + C+    Y  + + PS +CAG     + +C+GDSGGPL C++   RL + VG  S+  + C + NKP VYTRI+    WI
Sbjct:  197 VVTLKCSACGTRTGYSPRIVGGNMSSLTQWPWQVSLQFQGYHLCGGSIITPLWIVTAAHCVYDL-YHPKSWTVQVGLVSLMDSPVPSHL--------VEKIIYHSKYKPKRL---GNDIALMKLSEPLTFDET--IQPICLPNSEENFPDGKLCWTSGWGATE-DGGDASPVLNHAAVPLISNKICNHRDVYGGIIS-PSMLCAGYLKGGVDSCQGDSGGPLVCQE--RRLWKLVGATSFG-IGCAEVNKPGVYTRITSFLDWI 441          
BLAST of SMED30032182 vs. Ensembl Mouse
Match: Tmprss3 (transmembrane protease, serine 3 [Source:MGI Symbol;Acc:MGI:2155445])

HSP 1 Score: 124.79 bits (312), Expect = 9.221e-32
Identity = 91/264 (34.47%), Postives = 140/264 (53.03%), Query Frame = -1
Query:  142 VLTYNLSASGWK------IINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSP-GKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSK--TYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQ-VGIVSYSKMPCGQANKPTVYTRISEVAKWI 903
            V+T   SA G +      I+ G+ + + Q+P  VS+Q  G H+CGG+++    ++TAAHCV+D+ Y+   W     +  ++      H+        V KI+ H  YK  +    GND+ALMK    ++ DET  ++ I L    +  P GK C   GWG+ + DG D S  L  A + ++S + C+    Y  + + PS +CAG     + +C+GDSGGPL C++   RL + VG  S+  + C + NKP VYTRI+    WI
Sbjct:  199 VVTLKCSACGTRTGYSPRIVGGNMSSLTQWPWQVSLQFQGYHLCGGSIITPLWIVTAAHCVYDL-YHPKSWTVQVGLVSLMDSPVPSHL--------VEKIIYHSKYKPKRL---GNDIALMKLSEPLTFDET--IQPICLPNSEENFPDGKLCWTSGWGATE-DGGDASPVLNHAAVPLISNKICNHRDVYGGIIS-PSMLCAGYLKGGVDSCQGDSGGPLVCQE--RRLWKLVGATSFG-IGCAEVNKPGVYTRITSFLDWI 443          
BLAST of SMED30032182 vs. Ensembl Mouse
Match: Tmprss3 (transmembrane protease, serine 3 [Source:MGI Symbol;Acc:MGI:2155445])

HSP 1 Score: 124.79 bits (312), Expect = 9.221e-32
Identity = 91/264 (34.47%), Postives = 140/264 (53.03%), Query Frame = -1
Query:  142 VLTYNLSASGWK------IINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSP-GKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSK--TYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQ-VGIVSYSKMPCGQANKPTVYTRISEVAKWI 903
            V+T   SA G +      I+ G+ + + Q+P  VS+Q  G H+CGG+++    ++TAAHCV+D+ Y+   W     +  ++      H+        V KI+ H  YK  +    GND+ALMK    ++ DET  ++ I L    +  P GK C   GWG+ + DG D S  L  A + ++S + C+    Y  + + PS +CAG     + +C+GDSGGPL C++   RL + VG  S+  + C + NKP VYTRI+    WI
Sbjct:  199 VVTLKCSACGTRTGYSPRIVGGNMSSLTQWPWQVSLQFQGYHLCGGSIITPLWIVTAAHCVYDL-YHPKSWTVQVGLVSLMDSPVPSHL--------VEKIIYHSKYKPKRL---GNDIALMKLSEPLTFDET--IQPICLPNSEENFPDGKLCWTSGWGATE-DGGDASPVLNHAAVPLISNKICNHRDVYGGIIS-PSMLCAGYLKGGVDSCQGDSGGPLVCQE--RRLWKLVGATSFG-IGCAEVNKPGVYTRITSFLDWI 443          
BLAST of SMED30032182 vs. UniProt/SwissProt
Match: sp|Q9UKR3|KLK13_HUMAN (Kallikrein-13 OS=Homo sapiens OX=9606 GN=KLK13 PE=2 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 2.651e-35
Identity = 77/234 (32.91%), Postives = 126/234 (53.85%), Query Frame = -1
Query:  142 HQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYID-GNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 840
            H  P   ++ + G  +CGG L++   VLTAAHC+ +             +KV LG++ L  +   E+V +V   + HP Y+R+  +++  +D+ L++    +    T Y++ + L  + +++PG  C V GWG+      +  + LQ A + + S EEC + YP  +   + +CAG+      +C+GDSGGPL C    NR +  GIVS+   PCGQ ++P VYTR+S    WI
Sbjct:   45 HSQPWQAALLVQGRLLCGGVLVHPKWVLTAAHCLKE------------GLKVYLGKHALGRVEAGEQVREVVHSIPHPEYRRSPTHLNHDHDIMLLELQSPV--QLTGYIQTLPLSHNNRLTPGTTCRVSGWGTTTSPQVNYPKTLQCANIQLRSDEECRQVYPG-KITDNMLCAGTKEGGKDSCEGDSGGPLVC----NRTL-YGIVSWGDFPCGQPDRPGVYTRVSRYVLWI 258          
BLAST of SMED30032182 vs. UniProt/SwissProt
Match: sp|Q6UWB4|PRS55_HUMAN (Serine protease 55 OS=Homo sapiens OX=9606 GN=PRSS55 PE=1 SV=2)

HSP 1 Score: 129.413 bits (324), Expect = 3.315e-33
Identity = 87/249 (34.94%), Postives = 126/249 (50.60%), Query Frame = -1
Query:  142 KIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPG----KRCEVVGWGSMQ-PDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCR-DPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 870
            +I  G    + ++P  VSIQ   +  CGG++LN   +LTAAHC++    +        ++ VVLG  DL   S   ++ +V  I++H  +KRA      ND+AL+     I  D+      +K+   L   PG    + C V GWG     D       L KA + I+  EECSK +P +    + +CAG  + S  ACKGDSGGPL C  +P  +  QVGI+S+ K  CG+ N P +YT +     WI
Sbjct:   67 RITGGMEAEVGEFPWQVSIQARSEPFCGGSILNKWWILTAAHCLYSEELFP------EELSVVLGTNDL--TSPSMEIKEVASIILHKDFKRANM---DNDIALLLLASPIKLDD------LKVPICLPTQPGPATWRECWVAGWGQTNAADKNSVKTDLMKAPMVIMDWEECSKMFPKLTK--NMLCAGYKNESYDACKGDSGGPLVCTPEPGEKWYQVGIISWGKS-CGEKNTPGIYTSLVNYNLWI 295          
BLAST of SMED30032182 vs. UniProt/SwissProt
Match: sp|P35034|TRYP_PLEPL (Trypsin OS=Pleuronectes platessa OX=8262 PE=2 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 4.131e-33
Identity = 83/245 (33.88%), Postives = 117/245 (47.76%), Query Frame = -1
Query:  142 KIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDT--SQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 870
            +II G     H  P + S+   G H CGG L+N   VL+ AHC          W+  + ++V+LGE+DL    G E++++ + I+ HP Y          D+        I    T  V  I L T   V+ G  C V GWG+   DG++      LQ   + IV  E+C K+YP++ + P  +CAG    S  AC GDSG PL CR         G+VS+ +  C Q N P VY ++ E   WI
Sbjct:   22 RIIGGHECAAHSRPFMASLNY-GYHFCGGVLINNQWVLSVAHC----------WYNPYAMQVMLGEHDLRKFEGTEQLMKTDTIIWHPNYDYQTL-----DLTSCSSSSTILWKVTHAVAPIPLPTSCPVA-GTPCSVSGWGNTARDGDEVYLPTLLQCMDVPIVDEEQCMKSYPDMIS-PRMVCAGFMDGSRDACNGDSGSPLVCRGEV-----YGLVSWGQG-CAQPNYPGVYVKLCEFLGWI 242          
BLAST of SMED30032182 vs. UniProt/SwissProt
Match: sp|P80646|CTRB_GADMO (Chymotrypsin B OS=Gadus morhua OX=8049 PE=1 SV=1)

HSP 1 Score: 124.405 bits (311), Expect = 2.382e-32
Identity = 81/244 (33.20%), Postives = 130/244 (53.28%), Query Frame = -1
Query:  142 KIINGSRTVMHQYPQIVSIQL-DGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 870
            +I+NG   V H +P  VS+Q  +G H CGG+L+N N V+TAAHC  ++R Y          +V++GE+D       ++ +Q+ K  M  T+ +  +    ND++L+K               +   +D+  +PG +C   GWG  + +   T   LQ+A L ++S EECS+T+ N  N+ S++   +G+   ++C GDSGGPL C+   N    VGIVS+    C     P VY R++E+  W+
Sbjct:   15 RIVNGEEAVPHSWPWQVSLQQSNGFHFCGGSLINENWVVTAAHC--NVRTYH---------RVIVGEHD----KASDENIQILKPSMVFTHPKWDSRTINNDISLIKLASPAVLGTNVSPVCLGESSDV-FAPGMKCVTSGWGLTRYNAPGTPNKLQQAALPLMSNEECSQTWGN--NMISDVMICAGAAGATSCMGDSGGPLVCQK-DNVWTLVGIVSWGSSRC-SVTTPAVYARVTELRGWV 238          
BLAST of SMED30032182 vs. UniProt/SwissProt
Match: sp|P49864|GRAK_RAT (Granzyme K OS=Rattus norvegicus OX=10116 GN=Gzmk PE=1 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 1.499e-31
Identity = 85/264 (32.20%), Postives = 132/264 (50.00%), Query Frame = -1
Query:  142 VTFLVLTYNLSASGW--KIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEEC-SKTYPNVQNVPSN--ICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAK-WI 915
            + FLV    +S+  +  +II G     H  P + SIQ  G HICGG L++   VLTAAHC          +   H   VVLG + L      ++  ++ + +    +K        ND+ L+K     + +  ++V+ + L +   +  G +C+V GWGS +PD   TS  LQ+  + I+S + C S++Y N + V +   ICAG       +CKGDSGGPL C+   + L+  G        CG +NKP VYT +++  + WI
Sbjct:    8 LVFLVAGIYMSSESFHTEIIGGREVQPHSRPFMASIQYRGKHICGGVLIHPQWVLTAAHC----------YSRGHSPTVVLGAHSLSKNEPMKQTFEIKEFIPFSGFKSGT-----NDIMLIKLR--TAAELNKHVQLLHLRSKNYIRDGTKCQVTGWGSTKPDVLTTSDTLQEVTVTIISRKRCNSQSYYNHKPVITKDMICAGDRRGEKDSCKGDSGGPLICKGVFHALVSGG------YKCGISNKPGVYTLLTKKYQTWI 248          
BLAST of SMED30032182 vs. TrEMBL
Match: H2DRI7 (Trypsin-like serine protease OS=Dugesia japonica OX=6161 PE=2 SV=1)

HSP 1 Score: 251.521 bits (641), Expect = 7.893e-80
Identity = 113/201 (56.22%), Postives = 151/201 (75.12%), Query Frame = -1
Query:  130 MRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWITLNS 732
            M+ + ++ F+Y++++V L EYDL+ + G EK+ QV K ++HP+Y+   AY++GNDVAL+KFDGFISN + +Y+ Y +LD +    PG RCEVVGWGSM P G+D S+FLQKA ++I+S  EC + + NV +V SNICAGSG LS S+C+GDSGGPLFC D     IQVGIVSY  +PCGQ N P+VYTR+SEVA WI  N+
Sbjct:    1 MKSWNLNTFKYYQMEVYLAEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKFDGFISNGQYDYIHYNELDVNAIFKPGDRCEVVGWGSMLPPGQDISRFLQKAAINIISNSECGRIFSNVADVRSNICAGSGRLSTSSCRGDSGGPLFCTDGKRHHIQVGIVSYGTVPCGQPNIPSVYTRVSEVAGWIKANA 201          
BLAST of SMED30032182 vs. TrEMBL
Match: A0A1I8GT16 (Peptidase S1 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig010631g1 PE=3 SV=1)

HSP 1 Score: 156.762 bits (395), Expect = 4.517e-42
Identity = 94/265 (35.47%), Postives = 145/265 (54.72%), Query Frame = -1
Query:  142 FLVLTYNLSASGWK---IINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLH-HISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKL----DTDLKVSP-GKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 909
            FLVL   LS +      II+G      Q+P +VS+Q +G+H CGGTL +  TV+TAAHC     Y     F    + VV GE DL    S   +  +V+++++HP Y+ +  Y+ G+D+A++K    +S+D       IK     D  + + P G  C+V GWG++ P G           +  ++  +C++ +  V      +CAG G+ + +AC+GDSGGPL+C     +  QVGIVSY ++PCGQ   PTVYTR++   ++I
Sbjct:    3 FLVLFACLSVANAYDRLIIDGVEASTGQHPDLVSLQRNGNHFCGGTLYDKRTVVTAAHCF----YGNSGTF---GLAVVAGENDLTLSNSANRRSYRVSRVIIHPDYRSSYHYVQGDDIAILK----LSSDADLGTATIKAGDFEDAGVDIPPAGSSCKVAGWGAIDPSGRYAHTRKMIGGVSSITNNDCARVFNRVLGNTKQVCAGGGT-TTNACRGDSGGPLYC-TYNGKTRQVGIVSYGRVPCGQVGIPTVYTRVATYRRFI 254          
BLAST of SMED30032182 vs. TrEMBL
Match: A0A1I8JAW5 (Peptidase S1 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig007344g1 PE=3 SV=1)

HSP 1 Score: 155.606 bits (392), Expect = 1.203e-41
Identity = 94/265 (35.47%), Postives = 144/265 (54.34%), Query Frame = -1
Query:  142 FLVLTYNLSASGWK---IINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLH-HISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKL----DTDLKVSP-GKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 909
            FLVL   LS +      II+G      Q+P +VS+Q +G+H CGGTL +  TV+TAAHC     Y     F    + VV GE DL    S   +  +V+++++HP Y+ +  Y+ G+D+A++K    +S+D       IK     D  + + P G  C+V GWG++ P G           +  ++  +C++ +  V      +CAG G  + +AC+GDSGGPL+C     +  QVGIVSY ++PCGQ   PTVYTR++   ++I
Sbjct:    3 FLVLFACLSVANAYDRLIIDGVEASTGQHPDLVSLQRNGNHFCGGTLYDKRTVVTAAHCF----YGNSGTF---GLAVVAGENDLTLSNSANRRSYRVSRVIIHPDYRSSYHYVQGDDIAILK----LSSDADLGTATIKAGDFEDAGVDIPPEGSSCKVAGWGAIDPSGRYAHTRKMIGGVSSITNNDCARVFNRVLGNTKQVCAG-GDTTTNACRGDSGGPLYC-TYNGKTRQVGIVSYGRVPCGQVGIPTVYTRVATYRRFI 254          
BLAST of SMED30032182 vs. TrEMBL
Match: U5EP40 (Putative trypsin-like serine protease OS=Corethrella appendiculata OX=1370023 PE=2 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 1.700e-38
Identity = 88/265 (33.21%), Postives = 137/265 (51.70%), Query Frame = -1
Query:  136 YNLSASGWKIINGSRTVMHQYPQIVSIQLDGD----------HICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKF-DGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQ-PDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWITL 894
            Y+L A   KI+ GS    +QYP  +S+Q + +          H CGG+++N N +LTAAHC         +  E+  ++VV GE+ L      E+  QV++ ++H +Y      +  ND+A++K  + F+ N+    + + + D+     P     + GWGSM   +  +    LQ   L I+  EECS  +   Q   SN+CAG  + S S CKGDSGGPL   D    ++Q+G+VS+   PCG  +KP ++ R+S    WI L
Sbjct:   24 YSLEADH-KIVGGSNAEPNQYPYQISLQWNFNETVANPRRPFHFCGGSVINANWILTAAHC------KVANLSEFGFIEVVAGEHHLAFNDTTEQRRQVDRFILHESYGGG---VGPNDIAVIKVNESFVLNENVTRINFPERDS----IPSGNATLTGWGSMSITERPNAPNILQTVRLPIIEYEECSNLWNTTQLAASNVCAGELNGSKSGCKGDSGGPLVQTDGDLNVVQIGVVSWGSFPCGAEDKPGIFVRVSHFVNWIQL 274          
BLAST of SMED30032182 vs. TrEMBL
Match: A0A3B1IGC3 (Peptidase S1 domain-containing protein OS=Astyanax mexicanus OX=7994 PE=3 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 7.692e-36
Identity = 90/272 (33.09%), Postives = 144/272 (52.94%), Query Frame = -1
Query:  142 MNCKFYIVTFLVLTYNLSASGW--KIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGN---DVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDT--SQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 936
            + C   I+ F+  ++N     +  +++ G+  V  ++P   S+Q+ G HICGG L+    V++AAHC +D R Y+   +      V LG+  L   S  E+ L+++ I +H      + Y D N   D+AL++ D  +S         +   T  ++ PG  C V GWG+M+  GE    S  LQK  + +VS E C ++Y  +   P  +CAG  S    AC+GDSGGPL C++P+ R    G+VS+ +  CG+ +   VYTRI+ ++ WI
Sbjct:    4 LCCIEIILIFVDCSHNCGLRHFTTRVVGGADAVEGEWPWQASLQVSGRHICGGALIASQWVVSAAHCFYDDRLYSPSVW-----TVYLGKLRLRGTSQTEEALRLSHIHIH------QYYDDENHDYDLALLRLDRPVSAGTLAQPACLPSHTH-QLEPGLLCWVTGWGAMKEGGEKRGPSNVLQKVDVRLVSEEACVRSYGYIIT-PRMLCAGYRSGGKDACQGDSGGPLVCQEPSGRWFLAGVVSWGR-GCGRPDYYGVYTRITRLSDWI 261          
BLAST of SMED30032182 vs. Ensembl Cavefish
Match: ENSAMXT00000048530.1 (pep primary_assembly:Astyanax_mexicanus-2.0:13:20283923:20290642:1 gene:ENSAMXG00000040955.1 transcript:ENSAMXT00000048530.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 140.584 bits (353), Expect = 2.913e-39
Identity = 90/272 (33.09%), Postives = 144/272 (52.94%), Query Frame = -1
Query:  142 MNCKFYIVTFLVLTYNLSASGW--KIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGN---DVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDT--SQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 936
            + C   I+ F+  ++N     +  +++ G+  V  ++P   S+Q+ G HICGG L+    V++AAHC +D R Y+   +      V LG+  L   S  E+ L+++ I +H      + Y D N   D+AL++ D  +S         +   T  ++ PG  C V GWG+M+  GE    S  LQK  + +VS E C ++Y  +   P  +CAG  S    AC+GDSGGPL C++P+ R    G+VS+ +  CG+ +   VYTRI+ ++ WI
Sbjct:    4 LCCIEIILIFVDCSHNCGLRHFTTRVVGGADAVEGEWPWQASLQVSGRHICGGALIASQWVVSAAHCFYDDRLYSPSVW-----TVYLGKLRLRGTSQTEEALRLSHIHIH------QYYDDENHDYDLALLRLDRPVSAGTLAQPACLPSHTH-QLEPGLLCWVTGWGAMKEGGEKRGPSNVLQKVDVRLVSEEACVRSYGYIIT-PRMLCAGYRSGGKDACQGDSGGPLVCQEPSGRWFLAGVVSWGR-GCGRPDYYGVYTRITRLSDWI 261          
BLAST of SMED30032182 vs. Ensembl Cavefish
Match: ENSAMXT00000055551.1 (pep primary_assembly:Astyanax_mexicanus-2.0:13:20283717:20290642:1 gene:ENSAMXG00000040955.1 transcript:ENSAMXT00000055551.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 130.954 bits (328), Expect = 1.276e-35
Identity = 87/255 (34.12%), Postives = 137/255 (53.73%), Query Frame = -1
Query:  142 YNLSASGWKIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAH-CVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGN---DVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 894
            ++LSA  W   + S  +  ++P   S+Q+ G HICGG L+    V++AAH CV ++  Y+   +      V LG+  L   S  E+ L+++ I +H      + Y D N   D+AL++ D  +S         +   T  ++ PG  C V GWG+M+ +G   S  LQK  + +VS E C ++Y  +   P  +CAG  S    AC+GDSGGPL C++P+ R    G+VS+ +  CG+ +   VYTRI+ ++ WI
Sbjct:   16 FSLSAQAWPKRSKSH-LEGEWPWQASLQVSGRHICGGALIASQWVVSAAHFCVVNLHLYSPSVW-----TVYLGKLRLRGTSQTEEALRLSHIHIH------QYYDDENHDYDLALLRLDRPVSAGTLAQPACLPSHTH-QLEPGLLCWVTGWGAMK-EGGSPSNVLQKVDVRLVSEEACVRSYGYIIT-PRMLCAGYRSGGKDACQGDSGGPLVCQEPSGRWFLAGVVSWGRG-CGRPDYYGVYTRITRLSDWI 254          
BLAST of SMED30032182 vs. Ensembl Cavefish
Match: zgc:112160 (chymotrypsin B-like [Source:NCBI gene;Acc:103028134])

HSP 1 Score: 122.094 bits (305), Expect = 2.508e-32
Identity = 81/244 (33.20%), Postives = 124/244 (50.82%), Query Frame = -1
Query:  142 KIINGSRTVMHQYPQIVSIQ-LDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 870
            +I+NG   V H +P   S+Q   G H CGG+L+N N V+TAAHC  ++R            +++LGE++      K + L+VNK+  HP +         ND+AL+K     S + T     +    D+  +PG +C   GWG ++ +   T   LQ+  L ++S ++C K +       + ICAG+     S+C GDSGGPL C+   N    VGIVS+    C     P VY R++E+  W+
Sbjct:   33 RIVNGEEAVPHSWPWQASLQDFTGFHFCGGSLINENWVVTAAHC--NVRT---------SHRIILGEHNKAKSGEKIQTLRVNKVFTHPNWNPKTIE---NDIALVKLASPASIESTVSPVCLAGSNDV-FAPGLKCVTSGWGLLRHNALFTPDKLQQTALPLLSNDDC-KNHWGSNITGTMICAGAD--GASSCMGDSGGPLVCQKD-NVWTLVGIVSWGSSTC-STTTPAVYARVTELRAWV 256          
BLAST of SMED30032182 vs. Ensembl Cavefish
Match: ENSAMXT00000043830.1 (elastase-1-like [Source:NCBI gene;Acc:103036513])

HSP 1 Score: 120.939 bits (302), Expect = 7.961e-32
Identity = 90/255 (35.29%), Postives = 129/255 (50.59%), Query Frame = -1
Query:  142 KIINGSRTVMHQYPQIVSIQLDG------DHICGGTLLNGNTVLTAAHCVWDM--RYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFI-SNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEEC-SKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFC-RDPANRLIQVGIVSYSKMP-CGQANKPTVYTRISEVAKWI 870
            K++ GS      +    S+Q D        HICGG+L++   V+TAAHC+ DM  R+Y          +VVLG+YDL+   G E+   V++I+MHP +      ++GND+AL+K    +  N  TE   +   D  L    G  C + GWG +   G  T   LQ+A + +V    C S  + +     + +CAG G    S C+GDSGGPL C  D A R+   G+VS+     C    KPTV+TR+S    WI
Sbjct:   32 KVVGGSNAHPSMWRWQASLQYDNWNTGSFAHICGGSLISSLYVMTAAHCILDMQLRFY----------RVVLGDYDLYKYEGGEQPRSVSRIIMHPGW--TGDLVNGNDIALLKLSSPVYGNSYTEIANFPYPDQTLP--NGFTCYITGWGLLATGGT-TPDILQEAPIPVVEHSVCASPGWWSTAVKKTMVCAG-GDGKTSGCQGDSGGPLNCYTDGAWRV--HGVVSFGPASNCNIYTKPTVFTRVSSFMDWI 268          
BLAST of SMED30032182 vs. Ensembl Cavefish
Match: ENSAMXT00000006007.2 (chymotrypsin A-like [Source:NCBI gene;Acc:103028442])

HSP 1 Score: 120.168 bits (300), Expect = 1.335e-31
Identity = 82/245 (33.47%), Postives = 124/245 (50.61%), Query Frame = -1
Query:  142 KIINGSRTVMHQYPQIVSIQ-LDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFIS-NDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 870
            +I+NG   V H +P  VS+Q  +G H CGG+L+N + V+TAAHC   +R            +VVLGE+D    S   +++ V K+  HP +K        ND+ L+K       ND    V     +T+     G +C   GWG  + +  DT   LQ+A L +++ ++C   +   +     ICAG+     S+C GDSGGPL C+   NR   VGIVS+    C   + P VY R++++  W+
Sbjct:   33 RIVNGEDAVPHSWPWQVSLQNSEGFHFCGGSLINESWVVTAAHC--SVRTSN---------RVVLGEHDQSSDSEPIQIMSVGKVFKHPMFKHLN-----NDILLIKLSSPGQLNDHVSPV--CAAETNDTFPGGVKCVTTGWGLTKFNAPDTPAVLQQATLPLLTNDDCRHHW-GSRITEQMICAGAA--GASSCMGDSGGPLVCQK-DNRWTLVGIVSWGSGTC-STSMPAVYARVTQLRAWM 254          
BLAST of SMED30032182 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008289.1 (pep scaffold:Pmarinus_7.0:GL480450:20964:30734:1 gene:ENSPMAG00000007473.1 transcript:ENSPMAT00000008289.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 114.39 bits (285), Expect = 3.131e-30
Identity = 73/244 (29.92%), Postives = 115/244 (47.13%), Query Frame = -1
Query:  142 IINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKT--YPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 867
            I+ GS    H  P   S++ +G HICG  L+  N V+T+AHC          +     +KVVLG++ +    G EK   V++++ H  +       + NDV L+K    ++ +    ++ + L T   + PG  C V GWG++Q      S  L+   + IVS   C+ T  Y  +  V + +CAG       AC+GDSGGPL C +    L+  G      + C +     +YT +S +  WI
Sbjct:   26 IVGGSEVAPHAIPSQASLRYNGVHICGAALIRTNWVITSAHC----------YKAAQNLKVVLGDHSMSKADGTEKTFSVSRVVRHEKFNNVT---NDNDVMLVKLSANVALNAN--IQLVALQTS-ALPPGTLCNVSGWGTLQESSNKGSDVLRAVTVPIVSNALCNSTLYYRGLITV-NMLCAGFDQGGKDACQGDSGGPLICDNKLKGLVSWG------LGCARPRYFGIYTDVSRLISWI 246          
BLAST of SMED30032182 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005493.1 (pep scaffold:Pmarinus_7.0:GL476632:243920:250733:-1 gene:ENSPMAG00000004970.1 transcript:ENSPMAT00000005493.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 109.768 bits (273), Expect = 2.208e-28
Identity = 83/247 (33.60%), Postives = 117/247 (47.37%), Query Frame = -1
Query:  142 KIINGSRTVMHQYPQIVSIQLD-GDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLD-TDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPS-NICAGSGSLSISACKGDSGGPLFC-RDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 870
            +I+NG   +   +P  VS+Q   G H CGG+L++   V+TAAHC               + +V+LGE+D    S K + + + K + HP +         NDVAL+K         T+ V  + L  T     PG  C   GWG       DT   LQ+  L IV+T  C +   N+  +    ICAG      ++C+ DSGGPL C +D A  L  VGIVS+    C     P VYTR++ +  WI
Sbjct:   26 RIVNGEEAMPGSWPWQVSLQSKTGFHFCGGSLIDPYWVVTAAHCG----------ISPSEHRVILGEHDRSSSSEKVQTMTIAKAINHPQWDNMSI---NNDVALIKLSQ--PAQMTDGVSAVCLSSTSDNFPPGLNCVTSGWGITNNFDSDTPPRLQQTALAIVATPTCQQLLANMNKITDVMICAGGAGA--TSCRRDSGGPLVCEKDGAWNL--VGIVSWGNSSC-STRIPAVYTRVTAIRSWI 252          
BLAST of SMED30032182 vs. Ensembl Sea Lamprey
Match: si:dkey-33m11.8 (si:dkey-33m11.8 [Source:ZFIN;Acc:ZDB-GENE-141215-49])

HSP 1 Score: 109.383 bits (272), Expect = 2.459e-28
Identity = 83/253 (32.81%), Postives = 115/253 (45.45%), Query Frame = -1
Query:  142 GWKIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHC---VWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKF-DGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGS----MQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 876
            G +I+ G     +  P   S+Q +G H CGGTL+N   VLTAAHC     D+R              VLGE+ L ++ GKE+   V + + H  Y    A +D ND+ L+K     + N   + V+  +    L+   G  C V GWG+    + PD       L+  +LD  S   C+  Y       +  CA   +    AC+GDSGGPL C    N     GIVS+    C   N P VYT++S    WI
Sbjct:   19 GSRIVGGFEVARNSLPYQASLQQNGVHYCGGTLINAKWVLTAAHCEITSLDLR--------------VLGEHSLGNVEGKEQTFMVERAIRHAGYD--PATVD-NDIMLLKLPRAAVLNAAVQPVQLARAGARLE--DGTSCLVSGWGTVGNGVMPDA------LRAVYLDTTSRGYCNDAYSGTL-TDNMFCAVYAAGGRDACQGDSGGPLICNGALN-----GIVSWG-YGCAYPNYPGVYTKVSNYITWI 239          
BLAST of SMED30032182 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006511.1 (pep scaffold:Pmarinus_7.0:GL476457:197255:208031:1 gene:ENSPMAG00000005872.1 transcript:ENSPMAT00000006511.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 107.071 bits (266), Expect = 2.453e-27
Identity = 80/267 (29.96%), Postives = 127/267 (47.57%), Query Frame = -1
Query:  139 VTFLVLTYNLSASGWKIINGSRTVMHQYPQIVSIQLDGD----HICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKR--AKAYIDGNDVALMKFDGFISNDETEYVKYIKLD-TDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSN-ICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWIT 915
            +T L   +++SA   +I+NG     H +P   S+Q        H+CGG+L+    V+TAAHC+   R Y M          VLG+++L      +    +   ++HP++     + + D   +ALMK     +   +E +K   L     ++     C   GWG +  DG+  S  LQ+A L  V+   CS+       V +N ICAG      + C GDSGGPL C++P    +  GI S+    C   +KP+V+TR+S    W++
Sbjct:   15 LTSLTYLWHVSAQN-RIVNGVEAAPHTWPWQASLQAQTGTGWYHVCGGSLVQQQWVMTAAHCIQADRVYRM----------VLGKHNLRINEPAQFESTIALSVVHPSWNNHPTRGWFD---IALMKLSS--AAPLSEQIKLAVLPRVGQELWDNVTCMATGWGLLASDGKFPS-VLQEAKLPFVNYSTCSQQEWWWYGVNTNLICAGDDH---AVCNGDSGGPLSCQEPNGSWVVYGITSFGHKNCSVFHKPSVFTRVSAYTDWVS 261          
BLAST of SMED30032182 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002258.1 (pep scaffold:Pmarinus_7.0:GL487751:502724:507790:1 gene:ENSPMAG00000002029.1 transcript:ENSPMAT00000002258.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 106.301 bits (264), Expect = 4.583e-27
Identity = 78/244 (31.97%), Postives = 116/244 (47.54%), Query Frame = -1
Query:  142 KIINGSRTVMHQYPQIVSIQL-DGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 870
            ++ING   V   +P  VS+Q  DG H CGG++++   V+TAAHC           F+    ++++GE+D        ++L V K + HP Y    A    ND+ L+K        +T     +   TD   SPG    V GWG  + +    S  LQ+  L ++STE+C K + +    P  ICAG+     S C GDSGGPL  +      +  G+ S+    C   + P VYTR++    WI
Sbjct:   33 RVINGEEAVPGSWPWQVSLQYEDGFHFCGGSIIDPYWVITAAHCD----------FKPANHRIIVGEHDKSSNVENIQILYVEKAITHPLYD---ANTINNDILLLKLKEPAFFGDTVSPICLPRATD-TFSPGTHAAVTGWGVTRWNANYGSSTLQQTVLPLISTEDCKKWWGSYIMDPM-ICAGANGH--STCMGDSGGPLTVQKDG-MWVLAGLTSWGNSRC-DTSVPVVYTRVTTFVDWI 257          
BLAST of SMED30032182 vs. Ensembl Nematostella
Match: EDO44208 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RW61])

HSP 1 Score: 126.331 bits (316), Expect = 1.081e-34
Identity = 81/251 (32.27%), Postives = 129/251 (51.39%), Query Frame = -1
Query:  142 KIINGSRTVMHQYPQIVSIQ-LDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVK------VVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSG-SLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 870
            +IING     H +P  +S++   G H CGG+L++   V+TAAHC+             H+ K      VV+G +     +  ++ L++++I+ HP Y   +     ND+AL++    +  D    V  + L TD + +PGKRC + GWG++  D + +   LQ+A L I S E C + Y +V +  +++CAG   S +   C GDSGGPL C D  +  +  G VSY    C   +  TV+ R++    WI
Sbjct:    2 RIINGQNAQPHSWPWQISLRGRSGFHTCGGSLISDRWVVTAAHCI-------------HRNKNPGGYTVVVGAHKKRGSTSVQQSLRLSQIIEHPKYNDRRIV---NDIALIELATPVQFDSAGKVGTVCL-TDQRPAPGKRCYITGWGAINGDTQQSPDILQQAMLPIASHENCRRKYGDVSST-AHLCAGEARSDAAGGCNGDSGGPLVCEDNGSWFLH-GAVSYGLRNCPTTHY-TVFARVASYTDWI 232          
BLAST of SMED30032182 vs. Ensembl Nematostella
Match: EDO44207 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RW60])

HSP 1 Score: 124.79 bits (312), Expect = 5.218e-34
Identity = 79/245 (32.24%), Postives = 130/245 (53.06%), Query Frame = -1
Query:  142 KIINGSRTVMHQYPQIVSI-QLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSG-SLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 870
            +IING     H +P  +S+ Q    H+CGG L++   V+TA+HC+ D     ++   Y    VV+G + +   +  EK ++V++++ HP Y   +   D ND+AL++    +  D    V  + L T+ + +PGK+C + GWG++    + +   LQ+A L I S E C + Y +V +  +++CAG   S +   C GDSGGPL C D  +  +  G VSY    C   +  TV+ R++    WI
Sbjct:    2 RIINGQNAQPHSWPWQISLRQGRRFHLCGGALISDRWVVTASHCIHD----DLNPGSY---MVVVGAHRMSGSTSVEKTIRVSQVLEHPRYDSQR---DVNDIALIELATPVEFDSAGKVGTVCL-TNQRPAPGKQCYITGWGAVNGHTQQSPDILQQAMLPIASHENCRRKYGDVSST-AHLCAGEARSDAAGGCNGDSGGPLVCEDNGSWFLH-GAVSYGMKYCPTTHY-TVFARVASYTDWI 232          
BLAST of SMED30032182 vs. Ensembl Nematostella
Match: EDO38278 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDB3])

HSP 1 Score: 115.931 bits (289), Expect = 1.127e-30
Identity = 81/253 (32.02%), Postives = 130/253 (51.38%), Query Frame = -1
Query:  127 IINGSRTVMHQYPQIVSIQLDGD---HICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKL-DTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQN--VPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWITLNSK 867
            I+ G+     ++P  VS++L+     HICGG +++   VLTAAHCV D R           +K+ +GE+ L ++ G E+V+ V +I+ H  Y      +D  D AL+K    +  + T+YV+ + L D+D     G  C V GWGS    G  +  +LQ+  L +V+  +C  TY        P   CAG+  ++ + C GDSGGPL C +   R   +G+ S+  + C QA +P V++ +     WI   ++
Sbjct:    4 IMGGANAEHGEWPWQVSMKLNSSSLPHICGGNVISPWWVLTAAHCVQDERA--------SNIKLTMGEWRLFNVDGTEQVIPVERIISHANYSYNT--VD-YDYALLKLTRPL--NFTQYVQPVCLPDSDFPA--GTLCYVTGWGSTNYRGSPSPNYLQEVGLPLVNHSQCHATYLTASRKITPRMRCAGTEGVAKAVCSGDSGGPLVC-ERGGRWFLMGLSSWGWV-CPQA-RPKVFSDVLAAMDWIREKTR 238          
BLAST of SMED30032182 vs. Ensembl Nematostella
Match: EDO39910 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S8P7])

HSP 1 Score: 115.546 bits (288), Expect = 1.797e-30
Identity = 77/248 (31.05%), Postives = 122/248 (49.19%), Query Frame = -1
Query:  142 IINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKL----DTDLKVSPGKRCEVVGWG-SMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAG-SGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 867
            +I+GS    + +P +V I  +  H CGGTL++   V+TAAHCV     +  D   Y+++ + LGE+      G E+   V +I++HP Y    A    ND+AL+K      N      KY+ L        ++S GK C   GWG ++  D +  S  L++  L +V+ +EC   Y ++    + +C G  G  SIS C  DSGGP+ C+         G+VS+    C   +  +V  ++S+   WI
Sbjct:    1 MISGSDAQPNSWPWMVQINYNNGHHCGGTLVSPQWVVTAAHCV----DHVKDPKNYNELAITLGEHKRSASEGTEQRFSVARIIVHPQYFEPTAI--NNDIALIKL-----NKPARLNKYVNLACLPRQGEELSDGKICYATGWGLTVGGDWKSQSDVLKQTPLPVVNRQECQTDYDDIPITTAMMCTGYGGRSSISTCNTDSGGPVVCKSKLGHWYLQGVVSFGARACAPGHY-SVNAKVSKFVTWI 236          
BLAST of SMED30032182 vs. Ensembl Nematostella
Match: EDO34771 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNF5])

HSP 1 Score: 115.161 bits (287), Expect = 3.268e-30
Identity = 83/252 (32.94%), Postives = 125/252 (49.60%), Query Frame = -1
Query:  142 GWKIINGSRTVMHQYPQIVSIQLDGD-HICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHK-----VKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISA-CKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 876
            G ++ING     H +P  +S++  G  H CGGTL++   V+TA+HCV             HK       VV+G ++ +  +  ++ + V+ ++ HP Y   K     ND+AL++    +  D    V    L T+ + +PGKRC + GWGS    G ++ + LQ+A L I S  +C   Y  V +  +++CAG      S  C GDSGGPL C D   R    G VSY K+ C      TV+ R++    WI
Sbjct:   14 GARVINGQNAQPHSWPWQISLRPYGRYHSCGGTLISDRWVVTASHCV-------------HKNPRPSYTVVVGAHERNGKTAVQESIPVSHVIEHPEYDDRKIK---NDIALLELSRPVKFDREGKVGTACL-TNQQPTPGKRCYITGWGSTIGTG-NSPRILQQAMLPIASHNDCKNKYYGVSST-AHLCAGEARSGASGGCNGDSGGPLVCED-NGRWYLHGAVSYGKLHC-PTTYYTVFARVASYTDWI 244          
BLAST of SMED30032182 vs. Ensembl Medaka
Match: GZMM (duodenase-1 [Source:NCBI gene;Acc:101164468])

HSP 1 Score: 129.798 bits (325), Expect = 2.293e-35
Identity = 87/259 (33.59%), Postives = 123/259 (47.49%), Query Frame = -1
Query:  142 VTFLVLTYNLSASGWKIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISA-CKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 915
            V F++L +   A+G  I+ G     H  P + S+Q+ G HICGG L+  + VLTAAHC   + +            VVLG   L       +  +V + + HP Y         ND+ L+K DG      T  V+ I L +   V P  +C   GWG +  D    +  LQ+  + I++   C + + NV+ + S +C G G+ S    C GDSGGP+ C   A      GIVS+S   CG  + P VYTRIS    WI
Sbjct:    6 VMFVLLFFMGGAAGSHIVGGRDAAPHSRPYMASLQVRGQHICGGALVREDFVLTAAHCDLPIPF-----------TVVLGADLLQENEPSRQTFRVLRSIPHPNYNELS-----NDIMLLKLDG--RARLTPEVQVIPLKSG-TVGPASQCITAGWGDIG-DNRTIANRLQEVDVTILAQRTCRQRWGNVRVLRSMVC-GVGARSFQGFCSGDSGGPMICDGEA-----AGIVSFSGRRCGDPSTPDVYTRISSFRSWI 238          
BLAST of SMED30032182 vs. Ensembl Medaka
Match: TMPRSS6 (transmembrane serine protease 6 [Source:NCBI gene;Acc:101162577])

HSP 1 Score: 131.339 bits (329), Expect = 2.273e-33
Identity = 83/245 (33.88%), Postives = 134/245 (54.69%), Query Frame = -1
Query:  142 KIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTY-KRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDL-KVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 870
            +II G      ++P   S+Q+ G HICGG L++   V++AAHC +D R Y+   +      V LG+  L+  S  E+V +V  I +H  Y   ++ Y    D+AL+K D   S    E+ +   L     ++ P   C V GWG+++ +G   S  LQK  + +VS E+C ++Y ++ + P  +CAG  +    +C+GDSGGPL C++ + R    G+VS+ +  CG+ +   VYTRI+ +  WI
Sbjct:  566 RIIGGKDASEGEWPWQASLQVRGTHICGGALISSQWVVSAAHCFYDDRLYSPTMW-----TVYLGKLFLNRSSPTEEVARVQHIHLHQYYDDESQDY----DLALLKLDRPASALLAEHARPACLPPPTHQLEPDLLCWVTGWGALR-EGGGASNVLQKVDVRLVSEEDCVRSYGHLIS-PRMLCAGYRNGKKDSCQGDSGGPLVCQEASGRWFLAGVVSWGRG-CGRPDNYGVYTRITRLTDWI 798          
BLAST of SMED30032182 vs. Ensembl Medaka
Match: TMPRSS6 (transmembrane serine protease 6 [Source:NCBI gene;Acc:101162577])

HSP 1 Score: 130.954 bits (328), Expect = 2.948e-33
Identity = 83/245 (33.88%), Postives = 134/245 (54.69%), Query Frame = -1
Query:  142 KIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTY-KRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDL-KVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWI 870
            +II G      ++P   S+Q+ G HICGG L++   V++AAHC +D R Y+   +      V LG+  L+  S  E+V +V  I +H  Y   ++ Y    D+AL+K D   S    E+ +   L     ++ P   C V GWG+++ +G   S  LQK  + +VS E+C ++Y ++ + P  +CAG  +    +C+GDSGGPL C++ + R    G+VS+ +  CG+ +   VYTRI+ +  WI
Sbjct:  552 RIIGGKDASEGEWPWQASLQVRGTHICGGALISSQWVVSAAHCFYDDRLYSPTMW-----TVYLGKLFLNRSSPTEEVARVQHIHLHQYYDDESQDY----DLALLKLDRPASALLAEHARPACLPPPTHQLEPDLLCWVTGWGALR-EGGGASNVLQKVDVRLVSEEDCVRSYGHLIS-PRMLCAGYRNGKKDSCQGDSGGPLVCQEASGRWFLAGVVSWGRG-CGRPDNYGVYTRITRLTDWI 784          
BLAST of SMED30032182 vs. Ensembl Medaka
Match: ENSORLT00000023230.2 (tryptase [Source:NCBI gene;Acc:101157190])

HSP 1 Score: 118.242 bits (295), Expect = 1.064e-30
Identity = 88/259 (33.98%), Postives = 126/259 (48.65%), Query Frame = -1
Query:  133 GWKIINGSRTVMHQYPQIVSIQLDGDHI----CGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKL-DTDLKVSPGKRCEVVGWGSMQ-----PDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSY-SKMPCGQANKPTVYTRISEVAKWITLN 876
            G  II G      ++P +V + +  D +    CGG+L+    VLTAAHC WD            +  V +G  DL H  G E+  +V  ++ HP Y+        ND+AL++ D  ++   ++ V  + L  TD    P   C + GWG +      PD E     LQ+  + IV   +C +TYP + +    +CAGS +    ACKGD GGPL CR P    +QVGI+S  S   CG   +P VYTR+S    +I  N
Sbjct:   28 GSSIIGGEDAAEGKWPWMVHLNITSDGLKRWRCGGSLVAKQWVLTAAHC-WDPSLSV----NLRRSGVWVGARDLSH--GAERYRRVTHVISHPGYEGGGGVYK-NDLALLRLDKALAF--SQRVAPLSLVQTDDAFGPSSECWITGWGLVGNGVPLPDPET----LQQLQISIVPQRDCRQTYPWLTD--DMLCAGSPAGGKDACKGDYGGPLVCRTPRG-FVQVGIMSSGSPDGCGLPGRPGVYTRVSSYLAFIKSN 269          
BLAST of SMED30032182 vs. Ensembl Medaka
Match: prss56 (serine protease 56 [Source:NCBI gene;Acc:101155963])

HSP 1 Score: 122.865 bits (307), Expect = 1.940e-30
Identity = 81/251 (32.27%), Postives = 132/251 (52.59%), Query Frame = -1
Query:  142 NLSASGWKIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQV-GIVSYSKMPCGQANKPTVYTRISEVAKWI 891
            N++    +I+ GS      +P +V++QLDG  +CGG L++ + V+TAAHC    R  +  W        V+GE+D+      E+VL+VN+I+ HP +   K +   ND+AL++    +    +E+V  + L + +    G  C V GWGS+  DG  ++  + +A + ++    C           +  CAG  S  I +C+GDSGGPL  +D  +   Q+ GI S+    CG+  KP VYTR++  + WI
Sbjct:  184 NITQPRSRIVGGSPAPPGSWPWLVNLQLDGALMCGGVLVDSSWVVTAAHCFAGSRGES-SW------TAVVGEFDITKTDPDEQVLKVNRIISHPKFN-PKTF--NNDLALVELTSPVI--LSEHVTPVCLPSAMDPPTGTPCLVAGWGSLYEDGP-SADVVMEAKIPLLPQNTCKSALGKELVTSTMFCAGYLSGGIDSCQGDSGGPLIYQDLISGRFQLYGITSWGDG-CGEKGKPGVYTRVTAFSDWI 420          
BLAST of SMED30032182 vs. Planmine SMEST
Match: SMESG000042389.1 (SMESG000042389.1)

HSP 1 Score: 503.442 bits (1295), Expect = 0.000e+0
Identity = 239/239 (100.00%), Postives = 239/239 (100.00%), Query Frame = -1
Query:  127 MHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWITLNSK 843
            MHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWITLNSK
Sbjct:    1 MHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWITLNSK 239          
BLAST of SMED30032182 vs. Planmine SMEST
Match: SMESG000042388.1 (SMESG000042388.1)

HSP 1 Score: 325.094 bits (832), Expect = 1.119e-111
Identity = 146/239 (61.09%), Postives = 187/239 (78.24%), Query Frame = -1
Query:  127 MHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWITLNSK 843
            MH+YPQ+VSI++ G H CGGTLLNGNTVLTAAHCVW MR + ++ F+ + +K+ LGEYDL+ + G EK+ QV K+++HP+Y    AY+ GNDVAL+KFDG++SN +  Y+KY +LD +    PG RCEVVGWGSM P G+DTS+FLQK  ++I+S  ECS+ + NV+ V SN+CAGSGSLS SACKGDSGGPLFC D     IQVGIVSY ++PCGQAN PTV+TR+SEVA+WI  N+ 
Sbjct:    1 MHEYPQMVSIKVQGSHFCGGTLLNGNTVLTAAHCVWSMRNWNLNTFKSYSMKIYLGEYDLYQMYGVEKIFQVTKVIIHPSYTSDFAYVYGNDVALIKFDGYVSNTQYNYIKYGELDLNSITKPGDRCEVVGWGSMLPPGQDTSRFLQKTAINILSNSECSRIFTNVRYVASNVCAGSGSLSTSACKGDSGGPLFCTDGRRHHIQVGIVSYGRVPCGQANVPTVFTRVSEVARWINQNAN 239          
BLAST of SMED30032182 vs. Planmine SMEST
Match: SMESG000042349.1 (SMESG000042349.1)

HSP 1 Score: 324.324 bits (830), Expect = 1.815e-111
Identity = 147/239 (61.51%), Postives = 188/239 (78.66%), Query Frame = -1
Query:  127 MHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWITLNSK 843
            MH+YPQ+VSI++ G H CGGTLLNGNTVLTAAHCVW MR + ++ F+ + +K+ LGEYDL+ + G EK+ QV K+++HP+Y    AY+ GNDVAL+KFDG++SN +  Y+KY +LD +    PG RCEVVGWGSM P G+DTS+FLQKA ++I+ST ECS+ + NV  V SN+CAGSGSLS SAC+GDSGGPLFC D     IQVGIVSY ++PCGQAN PTV+TR+SEVA+WI  N+ 
Sbjct:    1 MHEYPQMVSIKVQGSHSCGGTLLNGNTVLTAAHCVWSMRNWNLNTFKSYSMKIYLGEYDLYQMYGVEKIFQVTKVIIHPSYTSDFAYVYGNDVALIKFDGYVSNTQYNYIKYGELDLNSITKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINILSTSECSRIFTNVWYVASNVCAGSGSLSTSACRGDSGGPLFCTDGRRHHIQVGIVSYGQVPCGQANVPTVFTRVSEVARWINQNAN 239          
BLAST of SMED30032182 vs. Planmine SMEST
Match: SMESG000042386.1 (SMESG000042386.1)

HSP 1 Score: 324.324 bits (830), Expect = 6.127e-111
Identity = 152/270 (56.30%), Postives = 202/270 (74.81%), Query Frame = -1
Query:  127 MNCKFYIVTFLVLTYNLSASGWKIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWITLNSK 936
            M+ K     F+VL++   ++  K INGS+ +MHQ+PQ+VSI+L  +HICGGTLLNGNTVLT AHC+WD+RY+  D+F+Y+K ++ LGEYDLH++ G EK  QV KI+MHP+Y    ++  GNDVAL+KFDG+I N + +Y+KY  LD +    PG RCEVVGWGS+ P GE+T++ LQ+A L ++S E+CSKT+ NV+ + SNICAG+G+   SACKGD GGPLFC D  N+ +QVGIVSY  +PCGQ   P V+TRIS VAKWI  +SK
Sbjct:    1 MSQKVVFHLFVVLSFCAISTALKTINGSQEIMHQFPQMVSIKLMDNHICGGTLLNGNTVLTTAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRISTVAKWIIDSSK 270          
BLAST of SMED30032182 vs. Planmine SMEST
Match: SMESG000042396.1 (SMESG000042396.1)

HSP 1 Score: 207.223 bits (526), Expect = 3.249e-66
Identity = 93/184 (50.54%), Postives = 135/184 (73.37%), Query Frame = -1
Query:  385 MNCKFYIVTFLVLTYNLSASGWKIINGSRTVMHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDI 936
            M  K  ++ FL+    +SA  WK+ING+++++H+YPQ+VSI+L G+HICGGTLLN NTVLTAAHC+W M  + ++  + +KV++VLGEYDL  +SG+EK+ QV K ++HP Y    +Y++GNDV L+KFDG + N+E +Y+KY +         G+RCEVVGWGS+ P G  +S+FLQKA L++
Sbjct:    1 MKFKNILIVFLIFISTVSAE-WKVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNV 183          
The following BLAST results are available for this feature:
BLAST of SMED30032182 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
KLK135.520e-3632.91kallikrein related peptidase 13 [Source:HGNC Symbo... [more]
PRSS556.903e-3434.94serine protease 55 [Source:HGNC Symbol;Acc:HGNC:30... [more]
PRSS557.654e-3434.94serine protease 55 [Source:HGNC Symbol;Acc:HGNC:30... [more]
CTRL6.784e-3233.33chymotrypsin like [Source:HGNC Symbol;Acc:HGNC:252... [more]
GZMM4.171e-3130.80granzyme M [Source:HGNC Symbol;Acc:HGNC:4712][more]
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BLAST of SMED30032182 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
svh-17.613e-2628.40HGF/MSP/plasminogen-like protein [Source:UniProtK... [more]
try-11.106e-2231.33TRYpsin-like protease [Source:UniProtKB/TrEMBL;Ac... [more]
try-33.715e-1427.66TRYpsin-like protease [Source:UniProtKB/TrEMBL;Ac... [more]
try-34.749e-1427.66TRYpsin-like protease [Source:UniProtKB/TrEMBL;Ac... [more]
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BLAST of SMED30032182 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG328082.440e-3235.19gene:FBgn0052808 transcript:FBtr0070295[more]
CG119112.422e-2932.81gene:FBgn0031249 transcript:FBtr0078098[more]
CG118369.637e-2833.33gene:FBgn0039272 transcript:FBtr0309271[more]
CG118361.622e-2733.33gene:FBgn0039272 transcript:FBtr0473579[more]
CG74321.655e-2733.20gene:FBgn0038727 transcript:FBtr0301213[more]
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BLAST of SMED30032182 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cela1.62.734e-3334.52chymotrypsin like elastase family member 1, tandem... [more]
cela1.62.917e-3334.52chymotrypsin like elastase family member 1, tandem... [more]
tmprss152.375e-3130.68transmembrane serine protease 15 [Source:ZFIN;Acc:... [more]
cela1.56.827e-3132.16chymotrypsin like elastase family member 1, tandem... [more]
cela1.58.677e-3131.76chymotrypsin like elastase family member 1, tandem... [more]
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BLAST of SMED30032182 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
il11ra2.302e-3432.95interleukin 11 receptor subunit alpha [Source:Xenb... [more]
il11ra8.555e-3432.95interleukin 11 receptor subunit alpha [Source:Xenb... [more]
ENSXETT00000030351.13.635e-3334.40transmembrane serine protease 11F [Source:NCBI gen... [more]
ENSXETT00000014775.13.763e-3334.40transmembrane serine protease 11F [Source:NCBI gen... [more]
ENSXETT00000030355.13.857e-3334.40transmembrane serine protease 11F [Source:NCBI gen... [more]
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BLAST of SMED30032182 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Klk133.249e-3330.71kallikrein related-peptidase 13 [Source:MGI Symbol... [more]
Tmprss38.054e-3234.47transmembrane protease, serine 3 [Source:MGI Symbo... [more]
Tmprss38.558e-3234.47transmembrane protease, serine 3 [Source:MGI Symbo... [more]
Tmprss39.221e-3234.47transmembrane protease, serine 3 [Source:MGI Symbo... [more]
Tmprss39.221e-3234.47transmembrane protease, serine 3 [Source:MGI Symbo... [more]
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BLAST of SMED30032182 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9UKR3|KLK13_HUMAN2.651e-3532.91Kallikrein-13 OS=Homo sapiens OX=9606 GN=KLK13 PE=... [more]
sp|Q6UWB4|PRS55_HUMAN3.315e-3334.94Serine protease 55 OS=Homo sapiens OX=9606 GN=PRSS... [more]
sp|P35034|TRYP_PLEPL4.131e-3333.88Trypsin OS=Pleuronectes platessa OX=8262 PE=2 SV=1[more]
sp|P80646|CTRB_GADMO2.382e-3233.20Chymotrypsin B OS=Gadus morhua OX=8049 PE=1 SV=1[more]
sp|P49864|GRAK_RAT1.499e-3132.20Granzyme K OS=Rattus norvegicus OX=10116 GN=Gzmk P... [more]
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BLAST of SMED30032182 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
H2DRI77.893e-8056.22Trypsin-like serine protease OS=Dugesia japonica O... [more]
A0A1I8GT164.517e-4235.47Peptidase S1 domain-containing protein OS=Macrosto... [more]
A0A1I8JAW51.203e-4135.47Peptidase S1 domain-containing protein OS=Macrosto... [more]
U5EP401.700e-3833.21Putative trypsin-like serine protease OS=Corethrel... [more]
A0A3B1IGC37.692e-3633.09Peptidase S1 domain-containing protein OS=Astyanax... [more]
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BLAST of SMED30032182 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000048530.12.913e-3933.09pep primary_assembly:Astyanax_mexicanus-2.0:13:202... [more]
ENSAMXT00000055551.11.276e-3534.12pep primary_assembly:Astyanax_mexicanus-2.0:13:202... [more]
zgc:1121602.508e-3233.20chymotrypsin B-like [Source:NCBI gene;Acc:10302813... [more]
ENSAMXT00000043830.17.961e-3235.29elastase-1-like [Source:NCBI gene;Acc:103036513][more]
ENSAMXT00000006007.21.335e-3133.47chymotrypsin A-like [Source:NCBI gene;Acc:10302844... [more]
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BLAST of SMED30032182 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000008289.13.131e-3029.92pep scaffold:Pmarinus_7.0:GL480450:20964:30734:1 g... [more]
ENSPMAT00000005493.12.208e-2833.60pep scaffold:Pmarinus_7.0:GL476632:243920:250733:-... [more]
si:dkey-33m11.82.459e-2832.81si:dkey-33m11.8 [Source:ZFIN;Acc:ZDB-GENE-141215-4... [more]
ENSPMAT00000006511.12.453e-2729.96pep scaffold:Pmarinus_7.0:GL476457:197255:208031:1... [more]
ENSPMAT00000002258.14.583e-2731.97pep scaffold:Pmarinus_7.0:GL487751:502724:507790:1... [more]
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BLAST of SMED30032182 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032182 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO442081.081e-3432.27Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO442075.218e-3432.24Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO382781.127e-3032.02Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO399101.797e-3031.05Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO347713.268e-3032.94Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30032182 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
GZMM2.293e-3533.59duodenase-1 [Source:NCBI gene;Acc:101164468][more]
TMPRSS62.273e-3333.88transmembrane serine protease 6 [Source:NCBI gene;... [more]
TMPRSS62.948e-3333.88transmembrane serine protease 6 [Source:NCBI gene;... [more]
ENSORLT00000023230.21.064e-3033.98tryptase [Source:NCBI gene;Acc:101157190][more]
prss561.940e-3032.27serine protease 56 [Source:NCBI gene;Acc:101155963... [more]
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BLAST of SMED30032182 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000042389.10.000e+0100.00SMESG000042389.1[more]
SMESG000042388.11.119e-11161.09SMESG000042388.1[more]
SMESG000042349.11.815e-11161.51SMESG000042349.1[more]
SMESG000042386.16.127e-11156.30SMESG000042386.1[more]
SMESG000042396.13.249e-6650.54SMESG000042396.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30032182 ID=SMED30032182|Name=SMED30032182|organism=Schmidtea mediterranea sexual|type=transcript|length=1026bp
TCAGAAAAGGACTTATATTTATTTGAAAAATTAAAGAAATTCAAACCATA
TATAAAACAACAATTACTCATTAGATTTCATTGTTGTTTTATTTTACAAT
TTCAAAAAAATTATTGAATCTGATCACTTTGAATTCAGCGTAATCCACTT
TGCAACTTCAGATATTCGTGTATAGACAGTCGGTTTATTTGCTTGGCCAC
AGGGCATTTTACTGTAACTTACAATTCCAACTTGAATGAGACGATTTGCT
GGATCTCTACAGAACAGAGGTCCTCCGGAGTCTCCTTTACAAGCAGATAT
TGATAAAGATCCACTGCCGGCACAGATATTGCTTGGTACATTTTGTACGT
TGGGATATGTTTTAGAACATTCTTCAGTGGAAACAATATCTAAAAAGGCT
TTTTGCAAAAATTGAGAGGTGTCTTCGCCATCTGGTTGCATACTGCCCCA
GCCTACAACTTCACAACGTTTTCCGGGACTTACTTTTAAATCAGTGTCCA
ATTTTATATACTTTACATATTCTGTTTCGTCATTTGAAATGAATCCATCA
AATTTCATTAACGCCACATCATTTCCATCAATATAAGCTTTTGCCCGTTT
ATATGTCGGATGCATCATAATTTTATTAACTTGAAGGACTTTTTCCTTTC
CACTTATGTGGTGCAAGTCATATTCACCGAGAACCACCTTTACTTTATGG
TATTCAAACCAGTCCATTGTATAATATCTCATATCCCAAACGCAATGTGC
GGCCGTGAGCACTGTATTTCCATTAAGTAGAGTCCCCCCACAAATATGGT
CACCGTCAAGCTGGATTGACACTATTTGTGGATACTGGTGCATTACTGTT
CGGCTTCCATTTATTATTTTCCACCCTGAGGCCGACAAATTGTAAGTAAG
AACTAAAAAAGTGACTATGTAAAATTTACAATTCATATTAAAATTAATTT
GTTGTTTTAATTCAATTTATTTACAATTGCATGGGAAACTGACATATTCT
TATTTTTTGTAGAACACATGTCGGAG
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protein sequence of SMED30032182-orf-1

>SMED30032182-orf-1 ID=SMED30032182-orf-1|Name=SMED30032182-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=271bp
MNCKFYIVTFLVLTYNLSASGWKIINGSRTVMHQYPQIVSIQLDGDHICG
GTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKV
LQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDL
KVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNV
PSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQAN
KPTVYTRISEVAKWITLNSK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR001314Peptidase_S1A
IPR001254Trypsin_dom
IPR033116TRYPSIN_SER
IPR018114TRYPSIN_HIS
IPR009003Peptidase_S1_PA
Vocabulary: molecular function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001314Peptidase S1A, chymotrypsin familyPRINTSPR00722CHYMOTRYPSINcoord: 119..133
score: 24.81
coord: 213..225
score: 61.81
coord: 50..65
score: 58.94
IPR001254Serine proteases, trypsin domainSMARTSM00020trypsin_2coord: 23..265
e-value: 2.5E-66
score: 236.3
IPR001254Serine proteases, trypsin domainPFAMPF00089Trypsincoord: 24..265
e-value: 5.8E-53
score: 180.0
IPR001254Serine proteases, trypsin domainPROSITEPS50240TRYPSIN_DOMcoord: 24..270
score: 29.57
IPR001254Serine proteases, trypsin domainCDDcd00190Tryp_SPccoord: 24..265
e-value: 2.49915E-68
score: 209.44
NoneNo IPR availableGENE3DG3DSA:2.40.10.10coord: 19..269
e-value: 1.1E-61
score: 210.3
NoneNo IPR availablePANTHERPTHR24276FAMILY NOT NAMEDcoord: 23..268
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..21
score: 0.627
IPR018114Serine proteases, trypsin family, histidine active sitePROSITEPS00134TRYPSIN_HIScoord: 60..65
IPR033116Serine proteases, trypsin family, serine active sitePROSITEPS00135TRYPSIN_SERcoord: 214..225
IPR009003Peptidase S1, PA clanSUPERFAMILYSSF50494Trypsin-like serine proteasescoord: 12..266