G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30032166
Length (bp)1249
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30032166) t-SNE clustered cells

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30032166) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30032166) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30032166) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30032166

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymaSMED30032166SMESG000042257.1 dd_Smed_v4_6240_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30032166SMESG000042257.1 dd_Smed_v4_6240_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30032166SMESG000042257.1 dd_Smed_v4_6240_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A267DN13 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig001214g1 PE=4 SV=1)

HSP 1 Score: 226.098 bits (575), Expect = 9.329e-66
Identity = 141/361 (39.06%), Postives = 210/361 (58.17%), Query Frame = 1 
Query:   79 IMNIGFNSSINRNLSKAEYNYYKIYYLNLEKYYLNWYLPITIFVXXXXXXVCATFLIISKIFPKNVLIWMLSICIGXXXXXXXXXXXXXXKVWIGFDIRDFNNTICKMHNFFSNYFIYWSGYMQTCMSIQRLISILNPLKARGMFTNKXXXXXXXXXXXXXTGPMVPYLLYWQVSEEKQGKDCSPVNKNLYTLITVLDITIWGIIPLLTMITSTIGISFVV-------FKST-------SEVGTFSSQSQSVKLVNNSSRRHAQVNNERAWQVTRLLICMNILYFISNFPLLIYQIYLN-AGGNKVDKSMPQQLHKTIYYTLRSICYLSSSWNWVFYCVGGHQFRTRALQIIRRFNCFHKT 1116
            ++N    +  NR   + E  +       L +Y+L WYLP+ + +G  G + C  F+ +S++FPK + +W++SIC GD MIL+ D LR+ LK+  G+D  D    ICK+ ++FS++F +WS Y Q  MSIQRL+ I +PL++R +   K V++ W +++ LL  P + YL+YW +  +    DC PV+K  Y + T++ +  WGIIPL +M+ ST  IS+ +       FK+        S+VG    Q   +       RRH    N     VTRLL+ MN+ Y  + FPLLIY +YLN    N+V KS+ + LHK  YY  RSIC+L++S NWVFYC  G  FR +A+QI+ R  C  K+
Sbjct:    3 LLNSSVETVGNRTDMQLEEEFGMRMLSGLTRYFLIWYLPLILTIGTFGAVFCMLFIFMSRMFPKIIQVWLVSICFGDFMILIWDVLRLFLKLAFGYDYIDQGVVICKLQSYFSSFFFHWSAYNQAAMSIQRLVFIASPLRSRTILNTKRVMTTWATMTFLLLFPNITYLIYWHIGRK---SDCEPVDKWFYYVTTLVHLIAWGIIPLASMVVSTGCISYRLIRYRRGNFKAAKTKDGRGSDVGGGVPQPAVI-----GGRRHGSDQNA---HVTRLLVSMNLFYLFTTFPLLIYMMYLNFVKDNQVGKSIAKSLHKFYYYLFRSICFLNASLNWVFYCAAGKVFRQKAMQILYRIFCREKS 352
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A267GDB7 (G_PROTEIN_RECEP_F1_2 domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig001214g3 PE=4 SV=1)

HSP 1 Score: 220.705 bits (561), Expect = 4.156e-63
Identity = 136/359 (37.88%), Postives = 207/359 (57.66%), Query Frame = 1 
Query:   79 IMNIGFNSSINRNLSKAEYNYYKIYYLNLEKYYLNWYLPITIFVXXXXXXVCATFLIISKIFPKNVLIWMLSICIGXXXXXXXXXXXXXXKVWIGFDIRDFNNTICKMHNFFSNYFIYWSGYMQTCMSIQRLISILNPLKARGMFTNKXXXXXXXXXXXXXTGPMVPYLLYWQVSEEKQGKDCSPVNKNLYTLITVLDITIWGIIPLLTMITSTIGISFVVFKSTSEVGTFSSQSQSVKLVNN-----------SSRRHAQVNNERAWQVTRLLICMNILYFISNFPLLIYQIYLN-AGGNKVDKSMPQQLHKTIYYTLRSICYLSSSWNWVFYCVGGHQFRTRALQIIRRFNCFHKTD 1119
            ++N    +  N+   + E  +       L +Y+L WYLP+ + +G  G + C  F+ +S++FPK + +W++SIC GD MIL+ D LR+ LK+  G+D  D    ICK+ ++FS++F +WS Y Q  MSIQRL+ I +PL++R +   K V++ W +++ LL  P + YL+YW +  +    DC PV+K  Y + T++ +  WGIIPL +M+ ST  IS+ + +     G F +        ++             RRH    N     VTRLL+ MN+ Y  + FPLLIY +YLN    N+V KS+ + LHK  YY  RSIC+L++S NWVFYC  G  FR +A+QI+ R  C  K+ 
Sbjct:   59 LLNSSVETVGNKTDMQLEEEFGMRMLSGLTRYFLIWYLPLILTIGTFGAVFCMLFIFMSRMFPKIIQVWLVSICFGDFMILIWDVLRLFLKLAFGYDYIDQGVVICKLQSYFSSFFFHWSAYNQAAMSIQRLVFIASPLRSRTILNTKRVMTTWATMTFLLLFPNITYLIYWHIGRK---SDCEPVDKWFYYVTTLVHLIAWGIIPLASMVVSTGCISYRLIRYRR--GNFKAAKTKDGRGSDGGGGVPQPAVIGGRRHGSDQNA---HVTRLLVSMNLFYLFTTFPLLIYMMYLNFVKDNQVGKSIAKSLHKFYYYLFRSICFLNASLNWVFYCAAGKVFRQKAMQILYRIFCREKSQ 409
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A267FL38 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig024275g2 PE=4 SV=1)

HSP 1 Score: 216.083 bits (549), Expect = 1.548e-61
Identity = 134/344 (38.95%), Postives = 199/344 (57.85%), Query Frame = 1 
Query:  130 EYNYYKIYYLNLEKYYLNWYLPITIFVXXXXXXVCATFLIISKIFPKNVLIWMLSICIGXXXXXXXXXXXXXXKVWIGFDIRDFNNTICKMHNFFSNYFIYWSGYMQTCMSIQRLISILNPLKARGMFTNKXXXXXXXXXXXXXTGPMVPYLLYWQVSEEKQGKDCSPVNKNLYTLITVLDITIWGIIPLLTMITSTIGISFVV-------FKSTSEVGTFSSQS--------QSVKLVNNSSRRHAQVNNERAWQVTRLLICMNILYFISNFPLLIYQIYLNAG-GNKVDKSMPQQLHKTIYYTLRSICYLSSSWNWVFYCVGGHQFRTRALQIIRRFNCFHK 1113
            E  +  ++Y  L  Y+L WYLP+ + +G +G + C  F+ +SK+FPK + +W++SIC GD MIL+ DALRM  K+   +D RD    ICK+  +  N+F +WS Y Q  MSIQRLI I  PL++R + T + V+ +W  + +LL  P + YL+YW + +     DC PV+K  Y + T++ + +WGIIPL +M  ST  ISF +       FK+ S+                +  +      RRH    +++   VTRLL+ MN+ Y  + FPLLIY ++LN    NKV  ++ + LHK+ YY  RSI +L++S NWVFYC  G  FR  A +I+ R  C H+
Sbjct:   28 EEEWGSLFYTRLNSYFLIWYLPLILLIGTLGAVFCTLFIFMSKMFPKIIQVWLISICFGDFMILMWDALRMFTKMAFDYDFRDSGVVICKLQAYLGNFFFHWSAYNQAAMSIQRLIFISFPLRSRTLLTTRRVIGIWACMGLLLLFPNITYLIYWHIGKR---DDCEPVDKWFYYVTTLVHLVVWGIIPLASMTISTGCISFRLIRYRRSNFKAKSDGVGSGGGGEAISGGALEPHQPAAAGGRRH---GSDQGAHVTRLLVSMNLFYLFTTFPLLIYMMHLNFRLENKVGVTIAKSLHKSYYYLFRSIAFLNASLNWVFYCAAGKIFRQAAKRILCRLFCPHR 365
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0R3X4B3 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS8225 PE=4 SV=1)

HSP 1 Score: 211.846 bits (538), Expect = 4.195e-60
Identity = 143/392 (36.48%), Postives = 205/392 (52.30%), Query Frame = 1 
Query:  121 SKAEYNYYKIYYLNLEKYYLNWYLPITIFVXXXXXXVCATFLIISKIFPKNVLIWMLSICIGXXXXXXXXXXXXXXKVWIGFDIRDFNNTICKMHNFFSNYFIYWSGYMQTCMSIQRLISILNPLKARGMFT-NKXXXXXXXXXXXXXTGPMVPYLLYWQVSEEKQGKDCSPVNKNLYTLITVLDITIWGIIPLLTMITSTI-----------------------------------------------GISFVVFKSTSEVGTFSS-------QSQ-------------SVKLVNNSSRRHAQVNNERAWQVTRLLICMNILYFISNFPLLIYQIYLNAGGNKVDKSMPQQLHKTIYYTLRSICYLSSSWNWVFYCVGGHQFRTRALQIIR 1092
            S+++   Y   Y  L  Y+L WYLP TI VG +G +    FL  S++F  N+L+W+ SICIGD  IL+L+ + +LLKVW  +DIRD N+ ICK+H   SNY +YWS YMQ+ +S+QR   +  PL ARG    +K  L  W  +S++L  P+ PY++YW++ +     DC P +++++ L T+ D+ IWGIIPLL M  +T+                                                I+ V+ +ST++  T SS       QS+               +L ++SS RH   +N     VTRLLICMNI Y  S FPLLI+ ++ N  G  VD       H+  YY  RS+C+L+S  NW+FYC+ G  FR  A  I+R
Sbjct:   10 SESQLRPYAYLYEELCIYFLRWYLPTTIIVGTMGTLFSLFFLFCSRLFQSNMLLWLSSICIGDFFILMLEGMWILLKVWFKYDIRDHNDLICKIHKAASNYALYWSAYMQSALSVQRAYLVFKPLHARGRGGISKRHLCAWTMISIILVAPVAPYIIYWRIID----GDCDPTDRDIFYLTTLCDLIIWGIIPLLGMTVATVVICLNMAKLGNKFKRAKVASSKGSRKFSVCITPEVPPFHRRRGLSTPSITTVLLRSTTDTQTLSSRLMDHLKQSEVGQSPMEYSSSQLERQLTDHSSSRHNAPDN--FGHVTRLLICMNIAYMASTFPLLIFLMFRNFSGVDVDP----DFHRFCYYLFRSLCFLNSCTNWIFYCLVGKLFREEAKHILR 391
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0R3W9Z6 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS7320 PE=4 SV=1)

HSP 1 Score: 211.075 bits (536), Expect = 7.295e-60
Identity = 145/385 (37.66%), Postives = 202/385 (52.47%), Query Frame = 1 
Query:  142 YKIYYLNLEKYYLNWYLPITIFVXXXXXXVCATFLIISKIFPKNVLIWMLSICIGXXXXXXXXXXXXXXKVWIGFDIRDFNNTICKMHNFFSNYFIYWSGYMQTCMSIQRLISILNPLKARGM-FTNKXXXXXXXXXXXXXTGPMVPYLLYWQVSEEKQGKDCSPVNKNLYTLITVLDITIWGIIPLLTMITSTI-----------------------------------------------GISFVVFKSTSEVGTFSS-------QSQSVK-------------LVNNSSRRHAQVNNERAWQVTRLLICMNILYFISNFPLLIYQIYLNAGGNKVDKSMPQQLHKTIYYTLRSICYLSSSWNWVFYCVGGHQFRTRALQIIR 1092
            Y   Y  L  Y+L WYLP TI VG +G +    FL  SK+F  N+L+W+ SICIGD  IL+L+ + +LLKVW  +DIRD N+ ICK+H   SNY +YWS YMQ+ +S+QR   +  PL ARG    +K  L  W+ +S++L  P+ PYL+YW++ +     DC P +++++ L T+ D+ IWGIIPLL M  ST+                                                I+ V+ KST++  T SS       QS++ +             L  ++S RH   +N     VTRLLICMNI Y  S FPLLI+ ++ N  G  VD       H+  YY  RS+C+L+S  NW+FYC+ G  FR  A  I+R
Sbjct:   18 YAYLYEELCIYFLRWYLPATIIVGTMGTLFSLFFLFCSKLFQSNMLLWLSSICIGDFFILMLEGMWILLKVWFKYDIRDHNDLICKIHKASSNYVLYWSAYMQSALSVQRAYLVFKPLHARGRGGISKKHLVAWIMISLILIAPVAPYLIYWRIID----GDCDPTDRDIFYLTTLCDLIIWGIIPLLGMTVSTVVICLNMAKLGNKFKRAKVASSKRPRKFSLCIPSEVPPAARRRGLSTPTITSVLLKSTTDTHTLSSRIMDHLQQSETAETQMEFSPNDLQRQLTEHNSSRHNAPDN--FGHVTRLLICMNIAYMASTFPLLIFLMFRNFSGVDVDP----DFHRFCYYLFRSLCFLNSCTNWIFYCLVGKLFREEAKHILR 392
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000042257.1 (SMESG000042257.1)

HSP 1 Score: 630.943 bits (1626), Expect = 0.000e+0
Identity = 345/356 (96.91%), Postives = 353/356 (99.16%), Query Frame = 1 
Query:   97 NSSINRNLSKAEYNYYKIYYLNLEKYYLNWYLPITIFVXXXXXXVCATFLIISKIFPKNVLIWMLSICIGXXXXXXXXXXXXXXKVWIGFDIRDFNNTICKMHNFFSNYFIYWSGYMQTCMSIQRLISILNPLKARGMFTNKXXXXXXXXXXXXXTGPMVPYLLYWQVSEEKQGKDCSPVNKNLYTLITVLDITIWGIIPLLTMITSTIGISFVVFKSTSEVGTFSSQSQSVKLVNNSSRRHAQVNNERAWQVTRLLICMNILYFISNFPLLIYQIYLNAGGNKVDKSMPQQLHKTIYYTLRSICYLSSSWNWVFYCVGGHQFRTRALQIIRRFNCFHKTDPQTETQTKTINLTSY 1164
            +S+I+ N +K ++++YKIYYLNLEKYYLNWYLPITIFVGIIGGIVCATFLIISKIFPKNVLIWMLSICIGDLMILLLDALRMLLKVWIGFDIRDFNNTICKMHNFFSNYFIYWSGYMQTCMSIQRLISILNPLKARGMFTNKLVLSVWVSVSVLLTGPMVPYLLYWQVSEEKQGKDCSPVNKNLYTLITVLDITIWGIIPLLTMITSTIGISFVVFKSTSEVGTFSSQSQSVKLVNNSSRRHAQVNNERAWQVTRLLICMNILYFISNFPLLIYQIYLNAGGNKVDKSMPQQLHKTIYYTLRSICYLSSSWNWVFYCVGGHQFRTRALQIIRRFNCFHKTDPQTETQTKTINLTSY
Sbjct:    2 SSNISENHNKTDFDFYKIYYLNLEKYYLNWYLPITIFVGIIGGIVCATFLIISKIFPKNVLIWMLSICIGDLMILLLDALRMLLKVWIGFDIRDFNNTICKMHNFFSNYFIYWSGYMQTCMSIQRLISILNPLKARGMFTNKLVLSVWVSVSVLLTGPMVPYLLYWQVSEEKQGKDCSPVNKNLYTLITVLDITIWGIIPLLTMITSTIGISFVVFKSTSEVGTFSSQSQSVKLVNNSSRRHAQVNNERAWQVTRLLICMNILYFISNFPLLIYQIYLNAGGNKVDKSMPQQLHKTIYYTLRSICYLSSSWNWVFYCVGGHQFRTRALQIIRRFNCFHKTDPQTETQTKTINLTSY 357
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000081941.1 (SMESG000081941.1)

HSP 1 Score: 197.978 bits (502), Expect = 3.072e-59
Identity = 130/335 (38.81%), Postives = 190/335 (56.72%), Query Frame = 1 
Query:  172 YYLNWYLPITIFVXXXXXXVCATFLIISKIFPKNVLIWMLSICIGXXXXXXXXXXXXXXKVWIGFDIRDFNNTICKMHNFFSNYFIYWSGYMQTCMSIQRLISILNPLKARGMFTNKXXXXXXXXXXXXXTGPMVPYLLYWQVSEEKQGKDCSPVNKNLYTLITVLDITIWGIIPLLTMITSTIGISFVVFKS------------TSEVGTFSSQSQSVKLVNNSSRR--------HAQVNNERAWQVTRLLICMNILYFISNFPLLIYQIYLNAGGNKVDKSMPQQLHKTIYYTLRSICYLSSSWNWVFYCVGGHQFRTRALQII----RRFNC 1104
            Y+L WYL I    G++G + C  FL +SK+FPK++L+W++SICIGD+MI++ + L M+ KV+  FD+RD N+ +C +H F SNYF YWS YMQ  MSIQR I  + P++AR +F  K  L +W +V++ +  P + Y++YW+V    +  DC P +   + + ++ D+ IWGIIPL TM  +T  I + + +              S     + Q+    L N   RR              E A  +T LLI MNI Y I+ +PLLIY + LN   N   +++    HK  YY  RSICYL++  NW+FYC  G  FR + ++II    R F C
Sbjct:   50 YFLRWYLIIVTTFGLLGTVFCLVFLHLSKLFPKSMLLWLVSICIGDMMIIINETLWMIGKVFYKFDLRDVNDVLCVLHTFLSNYFFYWSAYMQALMSIQRCIFTILPIRARFLFPYKRTLLIWFTVTMSIVFPHIYYIIYWRV----RNGDCDPPSSKYFGVTSLCDMIIWGIIPLGTMTITTAIICWAIRQPIENNLKIKAVSINSLCADINIQNHHPSLGNVEIRRGMNTCDVQKRNTKREAANHITYLLIFMNIWYIITTYPLLIYFMILNFTIN--SQTISSSSHKFYYYLFRSICYLNACSNWIFYCAAGKVFRNKTMEIIHQIYREFQC 378
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000035253.1 (SMESG000035253.1)

HSP 1 Score: 198.749 bits (504), Expect = 3.461e-59
Identity = 150/371 (40.43%), Postives = 209/371 (56.33%), Query Frame = 1 
Query:  151 YYLNLE---KYYLNWYLPITIFVXXXXXXVCATFLIISKIFPKNVLIWMLSICIGXXXXXXXXXXXXXXKVWIGFDIRDFNNTICKMHNFFSNYFIYWSGYMQTCMSIQRLISILNPLKARGMFTNKXXXXXXXXXXXXXTGPMVPYLLYWQVSEEKQGKDCSPVNKNLYTLITVLDITIWGIIPLLTMITST--IGISFVVFK--------STSEV---------GTFSSQSQSV----------------KLVN---------------NSSRRHAQVNNERAWQVTRLLICMNILYFISNFPLLIYQIYLNAGGNKVDKSMPQQLHKTIYYTLRSICYLSSSWNWVFYCVGGHQFRTRALQIIRRFNC 1104
            YYL      K+YL+W LP+ I VGI+G + C  FL+ SK+FPKN+L+W++SIC+ DLMILLLD L MLLK+WIGFDIRD NN +C  H F SNYF YWS YMQT +SIQR   I+ PL+AR       ++ VW+ +SVLL  P V Y++ W+V +     DC P + + + + ++ D+ IWGIIP L M+ ST  I I+   F+        ST +V          TF  ++Q+V                K +N               +  R+H  V+  +   VT+LLI MN+ Y ++ FPLLI+ IY+N     +D  +     K +YY LRS  + ++  NW+FYC  G  FRT A QI+ +  C
Sbjct:   21 YYLQFRMMCKFYLSWVLPMIIIVGIVGSVFCLIFLLFSKLFPKNLLLWLVSICVADLMILLLDGLWMLLKMWIGFDIRDINNFLCISHKFLSNYFFYWSAYMQTMVSIQRAYLIMRPLQARTSLVLPRLVIVWIGLSVLLIFPHVIYVVKWRVRK----GDCDPQDSSDFHVTSLCDLIIWGIIPFLAMVISTCIISINVCTFQSRTFAPRLSTPKVVYSFVRNNPSTFIGRTQTVNPSESSSNNSHSMKFIKQINYGHEGSPEFLSQNYVSPGRKHGGVDTAK--HVTQLLIGMNLWYLLTTFPLLIFLIYINF----IDPDINSWKRKFLYYLLRSFSFYNACSNWIFYCAAGKLFRTTAKQILIKMVC 381
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000058719.1 (SMESG000058719.1)

HSP 1 Score: 197.978 bits (502), Expect = 4.151e-59
Identity = 138/357 (38.66%), Postives = 197/357 (55.18%), Query Frame = 1 
Query:  169 KYYLNWYLPITIFVXXXXXXVCATFLIISKIFPKNVLIWMLSICIGXXXXXXXXXXXXXXKVWIGFDIRDFNNTICKMHNFFSNYFIYWSGYMQTCMSIQRLISILNPLKARGMFTNKXXXXXXXXXXXXXTGPMVPYLLYWQVSEEKQGKDCSPVNKNLYTLITVLDITIWGIIPLLTMITSTIGISFVVFK------------------------STSEVGTFSSQSQS---VKLVNNS------------SRRHAQVNNERAWQVTRLLICMNILYFISNFPLLIYQIYLNAGGNKVDKSMPQQLHKTIYYTLRSICYLSSSWNWVFYCVGGHQFRTRALQIIRRF---NCFHK 1113
            KYYL+W LP+ + VG++G I C  FL +SK+FP+ +L+W++SIC+GD MI++LD L MLLK+WIG DIRD N+ IC +H F SNYF YWS YMQ  MS+QR+  IL P++AR       +  +W  VS++L  P + YL +W++ +     DC P +   Y   T+ D+ IWGIIP L+M+ ST  I + + K                        S S   TF++Q+      K ++N+            S RH    N  A  VT+LL+ MNI Y I+ FP LI+ +YLN    K+D    +   K  Y+  RS  +L++  NW FYC  G  FR+ A  +I +     C H+
Sbjct:    3 KYYLSWILPLIMLVGVLGSIFCLLFLTMSKLFPRTLLLWLISICVGDFMIIILDTLWMLLKMWIGLDIRDVNDFICAVHKFLSNYFFYWSAYMQALMSLQRVYLILRPIQARSSLNMSRMTIIWGLVSIVLVLPHIIYLTHWRIID----GDCDPYDSKNYIFTTLCDMIIWGIIPFLSMVGSTSVICWNICKYQSNFYSSISKPSIIYSFVRSRTFSCSNHETFNTQNDMELLEKSIDNASIWSSPGSLRQQSSRHG--GNNVARHVTQLLVSMNIWYMITTFPFLIFLMYLNFVNPKID----EWTRKFYYFLFRSFAFLNACSNWFFYCATGKLFRSTAKMVIMKLFCRGCLHR 349
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000042263.1 (SMESG000042263.1)

HSP 1 Score: 131.724 bits (330), Expect = 4.721e-37
Identity = 91/105 (86.67%), Postives = 94/105 (89.52%), Query Frame = 1 
Query:   82 MNIGFNSSINRNLSKAEYNYYKIYYLNLEKYYLNWYLPITIFVXXXXXXVCATFLIISKIFPKNVLIWMLSICIGXXXXXXXXXXXXXXKVWIGFDIRDFNNTIC 396
            MNIGFNSSINRNLSKAEYNYYKIYYLNLEKYYL+WYLP+ IFVGIIGGIVC  FLIISKIFPKNVLIWMLSICIGDLMILL DALRML K+W  FDIRD   T C
Sbjct:    1 MNIGFNSSINRNLSKAEYNYYKIYYLNLEKYYLSWYLPVIIFVGIIGGIVCGIFLIISKIFPKNVLIWMLSICIGDLMILLFDALRMLFKMWFRFDIRDLTLTSC 105
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267DN139.329e-6639.06G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A267GDB74.156e-6337.88G_PROTEIN_RECEP_F1_2 domain-containing protein (Fr... [more]
A0A267FL381.548e-6138.95G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A0R3X4B34.195e-6036.48G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A0R3W9Z67.295e-6037.66G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000042257.10.000e+096.91SMESG000042257.1[more]
SMESG000081941.13.072e-5938.81SMESG000081941.1[more]
SMESG000035253.13.461e-5940.43SMESG000035253.1[more]
SMESG000058719.14.151e-5938.66SMESG000058719.1[more]
SMESG000042263.14.721e-3786.67SMESG000042263.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30032166 ID=SMED30032166|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1249bp
CGGAAATCCCAAAATCGATACTGATCATCAACGATACTTGACATGTTGAG
CATGAAATAATTGACTGCAAACTAAAAAATCATGAATATCGGATTCAATT
CAAGCATCAATCGGAATTTGAGCAAAGCGGAATATAATTATTATAAAATT
TATTATTTAAATTTGGAAAAATATTATCTGAACTGGTATTTACCGATAAC
GATTTTCGTTGGAATAATTGGTGGAATTGTTTGTGCAACATTTTTGATTA
TCTCGAAAATCTTTCCGAAAAACGTGCTGATTTGGATGCTGAGTATTTGC
ATTGGGGATTTGATGATTCTACTATTAGATGCTTTGAGAATGCTGCTGAA
AGTTTGGATTGGATTCGATATCCGGGATTTTAATAACACAATTTGCAAAA
TGCACAATTTCTTCTCCAATTATTTCATTTACTGGTCAGGATACATGCAA
ACTTGCATGTCAATTCAGAGATTAATTTCGATTCTAAATCCACTAAAAGC
CAGAGGTATGTTTACAAATAAATTAGTATTATCCGTTTGGGTCAGTGTAT
CGGTTTTGTTGACTGGTCCCATGGTACCATATTTACTTTACTGGCAAGTT
TCTGAAGAGAAGCAAGGAAAAGATTGTTCGCCAGTAAATAAAAATTTATA
CACTCTAATAACAGTTCTTGACATAACGATTTGGGGTATTATTCCTCTCT
TGACCATGATTACATCGACAATCGGCATCAGCTTTGTCGTGTTCAAGTCT
ACAAGCGAAGTCGGCACCTTCTCTAGCCAATCACAATCAGTTAAATTGGT
CAATAACAGTTCTCGACGACATGCGCAAGTCAACAACGAGAGAGCTTGGC
AAGTAACTAGGCTGTTGATTTGTATGAACATCTTATATTTTATCAGCAAT
TTCCCATTGCTTATTTATCAAATTTATCTTAATGCCGGTGGCAACAAAGT
TGACAAGTCCATGCCTCAACAACTGCACAAAACGATTTATTACACTTTAA
GAAGCATTTGTTATTTGAGCAGCTCCTGGAACTGGGTGTTTTATTGCGTC
GGCGGTCATCAATTTCGAACTCGTGCGTTGCAGATAATCCGACGTTTCAA
CTGTTTCCATAAAACTGATCCACAAACTGAAACACAAACGAAAACAATAA
ACCTTACTTCATATTGAAATCTCGATTGACCTTTTTGAAATTTGTGATGA
TTATTTAATATTTTATTTTTTTTTTCAGTTTCTTGAAATAAAATTCCAT
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protein sequence of SMED30032166-orf-1

>SMED30032166-orf-1 ID=SMED30032166-orf-1|Name=SMED30032166-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=362bp
MNIGFNSSINRNLSKAEYNYYKIYYLNLEKYYLNWYLPITIFVGIIGGIV
CATFLIISKIFPKNVLIWMLSICIGDLMILLLDALRMLLKVWIGFDIRDF
NNTICKMHNFFSNYFIYWSGYMQTCMSIQRLISILNPLKARGMFTNKLVL
SVWVSVSVLLTGPMVPYLLYWQVSEEKQGKDCSPVNKNLYTLITVLDITI
WGIIPLLTMITSTIGISFVVFKSTSEVGTFSSQSQSVKLVNNSSRRHAQV
NNERAWQVTRLLICMNILYFISNFPLLIYQIYLNAGGNKVDKSMPQQLHK
TIYYTLRSICYLSSSWNWVFYCVGGHQFRTRALQIIRRFNCFHKTDPQTE
TQTKTINLTSY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity