SMED30032157

Overview
NameSMED30032157
Smed IDSMED30032157
Length (bp)2334
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30032157 (SMED30032157) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30032157 (SMED30032157) in cells (dots) of each of the 12 neoblast clusters.

 

back to top


 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30032157 (SMED30032157) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30032157 (SMED30032157) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top


 

Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020




SMED30032157

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30032157h1SMcG0008950 Contig53551newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30032157h1SMcG0008950 Contig53551uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
protonephridiaSMED30032157h1SMcG0008950 dd_Smed_v4_2208_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30032157h1SMcG0008950 dd_Smed_v4_2208_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30032157h1SMcG0008950 dd_Smed_v4_2208_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30032157 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000508:24399..30012 +11424
Homology
BLAST of SMED30032157 vs. Ensembl Celegans
Match: unc-9 (Innexin unc-9 [Source:UniProtKB/Swiss-Prot;Acc:O01393])

HSP 1 Score: 194.126 bits (492), Expect = 7.312e-55
Identity = 125/391 (31.97%), Postives = 217/391 (55.50%), Query Frame = -3
Query:  287 SSLPVLNYKGLEDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYMSHTPGGENILKYLEHYCWVRGTMSLD-ESKIPQKSQDWHEFERSRIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLVKI----------SETTEPEKIASMIEHWL-VQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEVTNIFPRVAYCTARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHLF-LHSHNVSKSCEVEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKLFEKYQDKYQDQ 1420
            S++  + +   +D +DK+++  +  ++   +++V+ ++YV  PI C++  T   E + +Y E+YCWV+ T  L     IP    ++ E E  +I YYQWVPF+L  +A LF++P I+W ++    +G++V++LV++          S     + IA+ +E  L V+H+      +   +++L  ++ C   S    T+L   Y+ +K LY VN +GQI+L+  FL   N  ++   +GL++  +++ G+ WE +  FPRV  C    + +G V+  T QCVL  N+F EKI++FLWFW  L+A  T  SL  W++   VPSR  N V K+L  +  + +  S  + +F   FLR DG+FLL M+  ++G+L    +   L+  Y D  + +
Sbjct:   10 SAVKSIQFHVDDDIIDKLNYYYTTAIITVFAILVSAKQYVGFPIQCWVPAT-FTEPMEQYTENYCWVQNTYFLPLHDYIPH---NYAERENRQIGYYQWVPFVLALEALLFYVPTIVWRLLSWQ-SGIHVQSLVQMACDSRLLDLESRNRALQTIATNVEEALHVKHQ-----VMSGNRLKLLNLIICTRSSGAAVTFL---YISVKILYTVNIVGQIFLLNTFL--GNRSKW---YGLQVLNDLMNGREWEESGHFPRVTLCDFEVKVLGNVHRHTVQCVLMINMFNEKIFLFLWFWYFLLAGATLCSLFYWIYISVVPSRQLNFVGKYLTGIEGYKMVDSQSLRRFVFHFLRQDGVFLLRMVATHAGELPCYELAKTLWNNYCDNKEGK 382          
BLAST of SMED30032157 vs. Ensembl Celegans
Match: inx-1 (Innexin [Source:UniProtKB/TrEMBL;Acc:Q17394])

HSP 1 Score: 193.741 bits (491), Expect = 8.886e-55
Identity = 121/392 (30.87%), Postives = 207/392 (52.81%), Query Frame = -3
Query:  281 YKGL-----EDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYM--SHTPGGENILKYLEHYCWVRGTMSLD-ESKIPQKSQDWHEFERSR-IVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLVKIS----------ETTEPEKIASMIEHWLVQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEVTNIFPRVAYCTARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHLFLHSHNVSKSCEVEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKLFEKYQDKYQDQDF 1399
            +KGL     +DFVDK+++  +  ++ A ++IV+ ++YV  PI C++    T   E   +Y E+YCWV  T  L   S  P +  D     R+R I YYQWVPF+L  +A  F++P I+W  +    +G+NV++L +++               + IA  +E  L   RE                +  + + KR+A +++  Y+ +K LYL N + Q++++  FL  +N       +G  I ++++ G+ WEV+  FPRV  C    R +G V+  T QCVL  N+F EKI++FLWFW  +VA  ++ S+  W+   F+P +H   ++K+L        +   V+KF  +FL  DG+F + MI+ ++GD+    ++  L+  + D+ +   F
Sbjct:   10 FKGLHPRVDDDFVDKLNYHYTSAIIFAFAIIVSAKQYVGYPIQCWVPAQFTDAWE---QYTENYCWVENTYYLPLTSAFPLEYGD----RRARQISYYQWVPFVLALEALCFYIPCIMWRGLLHWHSGINVQSLTQMACDARMMDADARAATVQTIAGHMEDALEIQREVTD-------------VSGMCVQKRWANYVTLLYVFIKMLYLGNVVLQVFMLNSFLGTDN-----LFYGFHILRDLLNGREWEVSGNFPRVTMCDFEVRVLGNVHHHTVQCVLMINMFNEKIFLFLWFWYFMVAFVSAVSMFHWIIISFLPGQHMKFIRKYLRATDLATDRQ-SVKKFVHKFLGYDGVFCMRMISAHAGDILATELIVALWHNFNDRVRKIQF 375          
BLAST of SMED30032157 vs. Ensembl Celegans
Match: inx-1 (Innexin [Source:UniProtKB/TrEMBL;Acc:Q17394])

HSP 1 Score: 193.741 bits (491), Expect = 8.886e-55
Identity = 121/392 (30.87%), Postives = 207/392 (52.81%), Query Frame = -3
Query:  281 YKGL-----EDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYM--SHTPGGENILKYLEHYCWVRGTMSLD-ESKIPQKSQDWHEFERSR-IVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLVKIS----------ETTEPEKIASMIEHWLVQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEVTNIFPRVAYCTARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHLFLHSHNVSKSCEVEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKLFEKYQDKYQDQDF 1399
            +KGL     +DFVDK+++  +  ++ A ++IV+ ++YV  PI C++    T   E   +Y E+YCWV  T  L   S  P +  D     R+R I YYQWVPF+L  +A  F++P I+W  +    +G+NV++L +++               + IA  +E  L   RE                +  + + KR+A +++  Y+ +K LYL N + Q++++  FL  +N       +G  I ++++ G+ WEV+  FPRV  C    R +G V+  T QCVL  N+F EKI++FLWFW  +VA  ++ S+  W+   F+P +H   ++K+L        +   V+KF  +FL  DG+F + MI+ ++GD+    ++  L+  + D+ +   F
Sbjct:   10 FKGLHPRVDDDFVDKLNYHYTSAIIFAFAIIVSAKQYVGYPIQCWVPAQFTDAWE---QYTENYCWVENTYYLPLTSAFPLEYGD----RRARQISYYQWVPFVLALEALCFYIPCIMWRGLLHWHSGINVQSLTQMACDARMMDADARAATVQTIAGHMEDALEIQREVTD-------------VSGMCVQKRWANYVTLLYVFIKMLYLGNVVLQVFMLNSFLGTDN-----LFYGFHILRDLLNGREWEVSGNFPRVTMCDFEVRVLGNVHHHTVQCVLMINMFNEKIFLFLWFWYFMVAFVSAVSMFHWIIISFLPGQHMKFIRKYLRATDLATDRQ-SVKKFVHKFLGYDGVFCMRMISAHAGDILATELIVALWHNFNDRVRKIQF 375          
BLAST of SMED30032157 vs. Ensembl Celegans
Match: inx-1 (Innexin [Source:UniProtKB/TrEMBL;Acc:Q17394])

HSP 1 Score: 192.971 bits (489), Expect = 5.387e-54
Identity = 120/386 (31.09%), Postives = 205/386 (53.11%), Query Frame = -3
Query:  299 YKGL-----EDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYMSH--TPGGENILKYLEHYCWVRGTMSLD-ESKIPQKSQDWHEFERSR-IVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLVKIS----------ETTEPEKIASMIEHWLVQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEVTNIFPRVAYCTARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHLFLHSHNVSKSCEVEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKLFEKYQDK 1399
            +KGL     +DFVDK+++  +  ++ A ++IV+ ++YV  PI C++    T   E   +Y E+YCWV  T  L   S  P +  D     R+R I YYQWVPF+L  +A  F++P I+W  +    +G+NV++L +++               + IA  +E  L   RE                +  + + KR+A +++  Y+ +K LYL N + Q++++  FL  +N       +G  I ++++ G+ WEV+  FPRV  C    R +G V+  T QCVL  N+F EKI++FLWFW  +VA  ++ S+  W+   F+P +H   ++K+L        +   V+KF  +FL  DG+F + MI+ ++GD+    ++  L+  + D+
Sbjct:   10 FKGLHPRVDDDFVDKLNYHYTSAIIFAFAIIVSAKQYVGYPIQCWVPAQFTDAWE---QYTENYCWVENTYYLPLTSAFPLEYGD----RRARQISYYQWVPFVLALEALCFYIPCIMWRGLLHWHSGINVQSLTQMACDARMMDADARAATVQTIAGHMEDALEIQREVTD-------------VSGMCVQKRWANYVTLLYVFIKMLYLGNVVLQVFMLNSFLGTDN-----LFYGFHILRDLLNGREWEVSGNFPRVTMCDFEVRVLGNVHHHTVQCVLMINMFNEKIFLFLWFWYFMVAFVSAVSMFHWIIISFLPGQHMKFIRKYLRATDLATDRQ-SVKKFVHKFLGYDGVFCMRMISAHAGDILATELIVALWHNFNDR 369          
BLAST of SMED30032157 vs. Ensembl Celegans
Match: inx-11 (Innexin-11 [Source:UniProtKB/Swiss-Prot;Acc:Q23157])

HSP 1 Score: 192.2 bits (487), Expect = 2.823e-53
Identity = 126/422 (29.86%), Postives = 214/422 (50.71%), Query Frame = -3
Query:  200 EDFVDKMSFIASCILLIACSLIVTMREYVMDPITC-YMSHTPGGENILKYLEHYCWVRGTMSLDESKIPQ--KSQDWHEFERSRIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLVKISETTEPEKIASMIEHW--------LVQHREYRKGFVHNMKVRLYRIMKCLVI-----SKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEVTNIFPRVAYCTARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHL-------FLHSHNVSKSCEVEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKLFEKY--QDKYQD-QDFMDGDKFLLCKTGKNSELSTFVS*KARR 1387
            +D+ D+++++ +  +L+A S++++ +++   PI C + +  PG     +Y E+YCW + T  ++ ++     K ++ +  +R ++ YYQWVPF LL QAA F  P  LW     N +G      ++I E  E  K ++ +E          L +H      F  NM+ +  ++ K +       S  F +W+   YL  K LY +N   Q+YLM +FL  N H  Y    G  + ++I++G+ WE +  FPR A C    R V  +   + QCVL  N+F EKI++ LWFW V++ + ++ SL+ W   L  P      VK+HL       F   ++  +  +V KF +++L  DG+F+L M++ ++G +   ++V  L+E Y  QDK +D Q     D      TG  S +    + K  R
Sbjct:   20 DDWSDRLNYLMTPNILLAFSVLISFKQFGGRPIECMFPNKFPGSWE--QYAENYCWSQDTYFVEPTQDVSLLKKEERYTPDR-QLSYYQWVPFFLLLQAAFFRAPSYLWKYFS-NHSG------IRIHEVVEKAKDSANVEEEVREKNILILKRHLSSALRFQANMERKKVQVHKTVTFLNFQYSSGFISWI---YLFTKVLYFLNVFAQLYLMNYFLGTNRHHWY----GFGVVQDIVQGEPWERSGYFPRAAVCDFEVRQVANIQRYSVQCVLVINIFNEKIFVLLWFWYVILLLSSTVSLVQWFIVLVFPCFSKWFVKQHLALSTLQNFNQRNSRREDSDVSKFVTQYLHKDGVFVLRMVSSHAGIIFATDLVQALYEAYDFQDKNKDVQGSPVSDDLQTISTGAESSIRQRKTRKGSR 424          
BLAST of SMED30032157 vs. Ensembl Fly
Match: Inx2 (gene:FBgn0027108 transcript:FBtr0301860)

HSP 1 Score: 119.783 bits (299), Expect = 6.817e-29
Identity = 108/389 (27.76%), Postives = 177/389 (45.50%), Query Frame = -3
Query:  320 FDVVDKISSLPVLNYKGLEDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYMSHTPGGENILKYLEHYCWVRGTMSLDE-------SKIPQKSQDWH---EFERSRIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLV-KISETTEPEKIASMIEHWLVQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEV---TNIFPRVAYCT-ARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHLFLHSHNVSKSCEVEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKL 1441
            FDV   +  L  ++   +++ V +M + A+ I+LIA SL+VT R+Y+ DPI C +   P     L  ++ YCW+  T ++ E         + Q     H   E E     YYQWV F+L FQA LF++P+ LW     +  G  +K LV  ++     ++  +  +  LV   +Y  G ++      +R   C                  + L  VN IGQIY +  FL      E+S+ +G ++ K      +  +     +FP+V  CT  ++   G V      CVL  N+  EKIY+FLWFW ++++I +  SL+   +R+ V +       +HL L + +     E  +  +    +   FLLY +  N   L    ++S L
Sbjct:    2 FDVFGSVKGLLKIDQVCIDNNVFRMHYKATVIILIAFSLLVTSRQYIGDPIDCIVDEIP-----LGVMDTYCWIYSTFTVPERLTGITGRDVVQPGVGSHVEGEDEVKYHKYYQWVCFVLFFQAILFYVPRYLWK----SWEGGRLKMLVMDLNSPIVNDECKNDRKKILV---DYFIGNLNRHNFYAFRFFVC------------------EALNFVNVIGQIYFVDFFLD----GEFST-YGSDVLKFTELEPDERIDPMARVFPKVTKCTFHKYGPSGSVQTHDGLCVLPLNIVNEKIYVFLWFWFIILSIMSGISLI---YRIAVVA---GPKLRHLLLRARSRLAESEEVELVANKCNIGDWFLLYQLGKNIDPLIYKEVISDL 349          
BLAST of SMED30032157 vs. Ensembl Fly
Match: Inx2 (gene:FBgn0027108 transcript:FBtr0071005)

HSP 1 Score: 119.783 bits (299), Expect = 6.817e-29
Identity = 108/389 (27.76%), Postives = 177/389 (45.50%), Query Frame = -3
Query:  320 FDVVDKISSLPVLNYKGLEDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYMSHTPGGENILKYLEHYCWVRGTMSLDE-------SKIPQKSQDWH---EFERSRIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLV-KISETTEPEKIASMIEHWLVQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEV---TNIFPRVAYCT-ARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHLFLHSHNVSKSCEVEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKL 1441
            FDV   +  L  ++   +++ V +M + A+ I+LIA SL+VT R+Y+ DPI C +   P     L  ++ YCW+  T ++ E         + Q     H   E E     YYQWV F+L FQA LF++P+ LW     +  G  +K LV  ++     ++  +  +  LV   +Y  G ++      +R   C                  + L  VN IGQIY +  FL      E+S+ +G ++ K      +  +     +FP+V  CT  ++   G V      CVL  N+  EKIY+FLWFW ++++I +  SL+   +R+ V +       +HL L + +     E  +  +    +   FLLY +  N   L    ++S L
Sbjct:    2 FDVFGSVKGLLKIDQVCIDNNVFRMHYKATVIILIAFSLLVTSRQYIGDPIDCIVDEIP-----LGVMDTYCWIYSTFTVPERLTGITGRDVVQPGVGSHVEGEDEVKYHKYYQWVCFVLFFQAILFYVPRYLWK----SWEGGRLKMLVMDLNSPIVNDECKNDRKKILV---DYFIGNLNRHNFYAFRFFVC------------------EALNFVNVIGQIYFVDFFLD----GEFST-YGSDVLKFTELEPDERIDPMARVFPKVTKCTFHKYGPSGSVQTHDGLCVLPLNIVNEKIYVFLWFWFIILSIMSGISLI---YRIAVVA---GPKLRHLLLRARSRLAESEEVELVANKCNIGDWFLLYQLGKNIDPLIYKEVISDL 349          
BLAST of SMED30032157 vs. Ensembl Fly
Match: Inx2 (gene:FBgn0027108 transcript:FBtr0332314)

HSP 1 Score: 119.783 bits (299), Expect = 6.817e-29
Identity = 108/389 (27.76%), Postives = 177/389 (45.50%), Query Frame = -3
Query:  320 FDVVDKISSLPVLNYKGLEDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYMSHTPGGENILKYLEHYCWVRGTMSLDE-------SKIPQKSQDWH---EFERSRIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLV-KISETTEPEKIASMIEHWLVQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEV---TNIFPRVAYCT-ARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHLFLHSHNVSKSCEVEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKL 1441
            FDV   +  L  ++   +++ V +M + A+ I+LIA SL+VT R+Y+ DPI C +   P     L  ++ YCW+  T ++ E         + Q     H   E E     YYQWV F+L FQA LF++P+ LW     +  G  +K LV  ++     ++  +  +  LV   +Y  G ++      +R   C                  + L  VN IGQIY +  FL      E+S+ +G ++ K      +  +     +FP+V  CT  ++   G V      CVL  N+  EKIY+FLWFW ++++I +  SL+   +R+ V +       +HL L + +     E  +  +    +   FLLY +  N   L    ++S L
Sbjct:    2 FDVFGSVKGLLKIDQVCIDNNVFRMHYKATVIILIAFSLLVTSRQYIGDPIDCIVDEIP-----LGVMDTYCWIYSTFTVPERLTGITGRDVVQPGVGSHVEGEDEVKYHKYYQWVCFVLFFQAILFYVPRYLWK----SWEGGRLKMLVMDLNSPIVNDECKNDRKKILV---DYFIGNLNRHNFYAFRFFVC------------------EALNFVNVIGQIYFVDFFLD----GEFST-YGSDVLKFTELEPDERIDPMARVFPKVTKCTFHKYGPSGSVQTHDGLCVLPLNIVNEKIYVFLWFWFIILSIMSGISLI---YRIAVVA---GPKLRHLLLRARSRLAESEEVELVANKCNIGDWFLLYQLGKNIDPLIYKEVISDL 349          
BLAST of SMED30032157 vs. Ensembl Fly
Match: Inx2 (gene:FBgn0027108 transcript:FBtr0071006)

HSP 1 Score: 119.783 bits (299), Expect = 6.817e-29
Identity = 108/389 (27.76%), Postives = 177/389 (45.50%), Query Frame = -3
Query:  320 FDVVDKISSLPVLNYKGLEDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYMSHTPGGENILKYLEHYCWVRGTMSLDE-------SKIPQKSQDWH---EFERSRIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLV-KISETTEPEKIASMIEHWLVQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEV---TNIFPRVAYCT-ARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHLFLHSHNVSKSCEVEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKL 1441
            FDV   +  L  ++   +++ V +M + A+ I+LIA SL+VT R+Y+ DPI C +   P     L  ++ YCW+  T ++ E         + Q     H   E E     YYQWV F+L FQA LF++P+ LW     +  G  +K LV  ++     ++  +  +  LV   +Y  G ++      +R   C                  + L  VN IGQIY +  FL      E+S+ +G ++ K      +  +     +FP+V  CT  ++   G V      CVL  N+  EKIY+FLWFW ++++I +  SL+   +R+ V +       +HL L + +     E  +  +    +   FLLY +  N   L    ++S L
Sbjct:    2 FDVFGSVKGLLKIDQVCIDNNVFRMHYKATVIILIAFSLLVTSRQYIGDPIDCIVDEIP-----LGVMDTYCWIYSTFTVPERLTGITGRDVVQPGVGSHVEGEDEVKYHKYYQWVCFVLFFQAILFYVPRYLWK----SWEGGRLKMLVMDLNSPIVNDECKNDRKKILV---DYFIGNLNRHNFYAFRFFVC------------------EALNFVNVIGQIYFVDFFLD----GEFST-YGSDVLKFTELEPDERIDPMARVFPKVTKCTFHKYGPSGSVQTHDGLCVLPLNIVNEKIYVFLWFWFIILSIMSGISLI---YRIAVVA---GPKLRHLLLRARSRLAESEEVELVANKCNIGDWFLLYQLGKNIDPLIYKEVISDL 349          
BLAST of SMED30032157 vs. Ensembl Fly
Match: shakB (gene:FBgn0085387 transcript:FBtr0112571)

HSP 1 Score: 93.5893 bits (231), Expect = 4.662e-20
Identity = 84/308 (27.27%), Postives = 143/308 (46.43%), Query Frame = -3
Query:  563 DVVDKISSLPVLNYKGLEDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYMSHTPG-GENILKYLEHYCWVRGTMSLDESKIPQK----------SQDWHEFERSRIVYYQWVPFMLLFQAALFHLPKILW-TVICLNVTGLNVKNLVKISETTEPEKIASMIEHWLVQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEVTN---IFPRVAYCTA-RFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFW 1438
            D+   + +L  +++   +  V ++ +  + ++L++ SLI+T R+YV +PI C   HT    E++L     YCW++ T +L    + ++          + D    ++    YYQWV F L FQA LF+ P+ LW +     +  L +   + I    E ++   ++  +L ++  Y     HN                    W +  Y + + L L+N IGQ++LM  F       E+ + FGL++   +   Q   +     IFPR+  CT  ++   G V    A C+L  N+  EKIYIFLWFW
Sbjct:    3 DIFRGLKNLVKVSHVKTDSIVFRLHYSITVMILMSFSLIITTRQYVGNPIDC--VHTKDIPEDVLN---TYCWIQSTYTLKSLFLKKQGVSVPYPGIGNSDGDPADKKHYKYYQWVCFCLFFQAILFYTPRWLWKSWEGGKIHALIMDLDIGICSEAEKKQKKKLLLDYLWENLRY-----HN--------------------WWAYRYYVCELLALINVIGQMFLMNRFFD----GEFIT-FGLKVIDYMETDQEDRMDPMIYIFPRMTKCTFFKYGSSGEVEKHDAICILPLNVVNEKIYIFLWFW 275          
BLAST of SMED30032157 vs. UniProt/SwissProt
Match: sp|O01393|UNC9_CAEEL (Innexin unc-9 OS=Caenorhabditis elegans OX=6239 GN=unc-9 PE=1 SV=1)

HSP 1 Score: 194.126 bits (492), Expect = 9.302e-54
Identity = 125/391 (31.97%), Postives = 217/391 (55.50%), Query Frame = -3
Query:  287 SSLPVLNYKGLEDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYMSHTPGGENILKYLEHYCWVRGTMSLD-ESKIPQKSQDWHEFERSRIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLVKI----------SETTEPEKIASMIEHWL-VQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEVTNIFPRVAYCTARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHLF-LHSHNVSKSCEVEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKLFEKYQDKYQDQ 1420
            S++  + +   +D +DK+++  +  ++   +++V+ ++YV  PI C++  T   E + +Y E+YCWV+ T  L     IP    ++ E E  +I YYQWVPF+L  +A LF++P I+W ++    +G++V++LV++          S     + IA+ +E  L V+H+      +   +++L  ++ C   S    T+L   Y+ +K LY VN +GQI+L+  FL   N  ++   +GL++  +++ G+ WE +  FPRV  C    + +G V+  T QCVL  N+F EKI++FLWFW  L+A  T  SL  W++   VPSR  N V K+L  +  + +  S  + +F   FLR DG+FLL M+  ++G+L    +   L+  Y D  + +
Sbjct:   10 SAVKSIQFHVDDDIIDKLNYYYTTAIITVFAILVSAKQYVGFPIQCWVPAT-FTEPMEQYTENYCWVQNTYFLPLHDYIPH---NYAERENRQIGYYQWVPFVLALEALLFYVPTIVWRLLSWQ-SGIHVQSLVQMACDSRLLDLESRNRALQTIATNVEEALHVKHQ-----VMSGNRLKLLNLIICTRSSGAAVTFL---YISVKILYTVNIVGQIFLLNTFL--GNRSKW---YGLQVLNDLMNGREWEESGHFPRVTLCDFEVKVLGNVHRHTVQCVLMINMFNEKIFLFLWFWYFLLAGATLCSLFYWIYISVVPSRQLNFVGKYLTGIEGYKMVDSQSLRRFVFHFLRQDGVFLLRMVATHAGELPCYELAKTLWNNYCDNKEGK 382          
BLAST of SMED30032157 vs. UniProt/SwissProt
Match: sp|Q23157|INX11_CAEEL (Innexin-11 OS=Caenorhabditis elegans OX=6239 GN=inx-11 PE=3 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 3.591e-52
Identity = 126/422 (29.86%), Postives = 214/422 (50.71%), Query Frame = -3
Query:  200 EDFVDKMSFIASCILLIACSLIVTMREYVMDPITC-YMSHTPGGENILKYLEHYCWVRGTMSLDESKIPQ--KSQDWHEFERSRIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLVKISETTEPEKIASMIEHW--------LVQHREYRKGFVHNMKVRLYRIMKCLVI-----SKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEVTNIFPRVAYCTARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHL-------FLHSHNVSKSCEVEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKLFEKY--QDKYQD-QDFMDGDKFLLCKTGKNSELSTFVS*KARR 1387
            +D+ D+++++ +  +L+A S++++ +++   PI C + +  PG     +Y E+YCW + T  ++ ++     K ++ +  +R ++ YYQWVPF LL QAA F  P  LW     N +G      ++I E  E  K ++ +E          L +H      F  NM+ +  ++ K +       S  F +W+   YL  K LY +N   Q+YLM +FL  N H  Y    G  + ++I++G+ WE +  FPR A C    R V  +   + QCVL  N+F EKI++ LWFW V++ + ++ SL+ W   L  P      VK+HL       F   ++  +  +V KF +++L  DG+F+L M++ ++G +   ++V  L+E Y  QDK +D Q     D      TG  S +    + K  R
Sbjct:   20 DDWSDRLNYLMTPNILLAFSVLISFKQFGGRPIECMFPNKFPGSWE--QYAENYCWSQDTYFVEPTQDVSLLKKEERYTPDR-QLSYYQWVPFFLLLQAAFFRAPSYLWKYFS-NHSG------IRIHEVVEKAKDSANVEEEVREKNILILKRHLSSALRFQANMERKKVQVHKTVTFLNFQYSSGFISWI---YLFTKVLYFLNVFAQLYLMNYFLGTNRHHWY----GFGVVQDIVQGEPWERSGYFPRAAVCDFEVRQVANIQRYSVQCVLVINIFNEKIFVLLWFWYVILLLSSTVSLVQWFIVLVFPCFSKWFVKQHLALSTLQNFNQRNSRREDSDVSKFVTQYLHKDGVFVLRMVSSHAGIIFATDLVQALYEAYDFQDKNKDVQGSPVSDDLQTISTGAESSIRQRKTRKGSR 424          
BLAST of SMED30032157 vs. UniProt/SwissProt
Match: sp|Q03412|UNC7_CAEEL (Innexin unc-7 OS=Caenorhabditis elegans OX=6239 GN=unc-7 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 5.455e-48
Identity = 111/377 (29.44%), Postives = 205/377 (54.38%), Query Frame = -3
Query:  302 EDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYMSHTPGGENILKYLEHYCWVRGTMSLD-ESKIPQKSQDWHEFERSRIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLVKISETTE--PEKIASMIEHWLVQHREY--------RKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEVTNIFPRVAYCTARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHL-FLHSH---NVSKSCEVEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKLFEKYQD 1387
            +DFVDK+++  +  +L + +L+V+ ++YV  PI C++  T   + + +Y E+YCWV+ T  +  +  IP++    +     +I YYQWVPF+L  +A LF++P ILW  +    +G+N++ LV+++        +I +   + + +H +         R+G   +    L     C    +    +++  Y+ +K LY  N + Q +L+ H L  N+       +G  + K+++    WE T +FPRV  C    R +G ++  T QCVL  N+F EKI++FLWFW +   I T  + + W+  +F+PS+  + V+K+L  L  H    ++    + KF + FLR DG+F+L MI+ ++G+L +  ++  L++ + +
Sbjct:  139 DDFVDKLNYYYTTTILASFALLVSAKQYVGFPIQCWVPAT-FTDAMEQYTENYCWVQNTYWVPMQEDIPREI---YSRRNRQIGYYQWVPFILAIEALLFYVPCILWRGLLYWHSGINLQGLVQMACDARLMDSEIKTRTVYTMARHMQDEVQLTNIDRQGHSRSCFSNLQLGANC---GRHCGCYVTMLYIGIKVLYSANVLLQFFLLNHLLGSNDLA-----YGFSLLKDLMHAIEWEQTGMFPRVTLCDFEVRVLGNIHRHTVQCVLMINMFNEKIFLFLWFWFLTCGIVTVCNTMYWILIMFIPSQGMSFVRKYLRVLPDHPAKPIADDVTLRKFTNNFLRKDGVFMLRMISTHAGELMSSELILALWQDFNN 503          
BLAST of SMED30032157 vs. UniProt/SwissProt
Match: sp|A8WVX4|INX19_CAEBR (Innexin-19 OS=Caenorhabditis briggsae OX=6238 GN=inx-19 PE=3 SV=3)

HSP 1 Score: 164.466 bits (415), Expect = 1.073e-42
Identity = 110/411 (26.76%), Postives = 201/411 (48.91%), Query Frame = -3
Query:  299 SSLPVLNYKGLEDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYMSHTPGGENILKYLEHYCWVRGTMSLDE-SKIPQKSQDWHEFERSRIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLVKIS-------ETTEPEKIASMIEHWLVQH--------REYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEVTNIFPRVAYCTARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHLFLHS-------HNVSKSCEVEK--------------FCSEFLRLDGIFLLYMINLNSGDLATGNIVSKLFEKYQDK 1420
            S +  L+ +G +D VD++++  + ++L  C L+++ ++Y   PI C++ +    E++ +Y+E YCW++ T  +     +P    D    E  +I YYQWVPF+L+ +A +F LP I W  +C   +GLN++ L+  +       + ++ +K    I    V +        R   +G++  +K   +   +C+ I           Y   K LY VN + Q +++   L+ +        FG ++  +I  G+ W  T  FPRV  C    R++  +N  T QC L  N+  EK++ FLW W +++AI T+ S + W+   F+ S   + V K + +             K   VE+              F S+FL+ DGI +L MI+ ++GD+    +V  L+++Y+++
Sbjct:   10 SFISALSVRGDDDAVDRLNYYYTPLILAVCCLVISAKQYGGTPIECWV-NPHSRESMEEYIESYCWIQNTYWIPMYENVPD---DHTAREEKQIGYYQWVPFILIAEALMFSLPCIFWR-LCSFQSGLNIQTLINAACDAQALLDYSDRQKAVEAITCNFVDNLDLQSPNGRIRARGWIARIKFSRFLSGQCISIV----------YSFTKLLYSVNVVAQFFILNACLKSSEF----VFFGFQVLSDIWAGRPWTETGHFPRVTLCDFEVRYLANLNRYTVQCALLINIINEKVFAFLWCWYMILAIITTCSFIYWIANSFIHSEKVDYVMKFIQIAESSEYKKLQKFEKDATVERLYTVIAFAPHLLDSFVSDFLKSDGILMLRMISNHAGDMIVVQLVRNLWQEYRER 401          
BLAST of SMED30032157 vs. UniProt/SwissProt
Match: sp|Q19746|INX3_CAEEL (Innexin-3 OS=Caenorhabditis elegans OX=6239 GN=inx-3 PE=1 SV=2)

HSP 1 Score: 162.925 bits (411), Expect = 2.672e-42
Identity = 107/378 (28.31%), Postives = 191/378 (50.53%), Query Frame = -3
Query:  272 KGLEDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYMSHTPGGENILKYLEHYCWVRGTMSLDE-SKIPQKSQDWHEFERSRIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLVKISETTEPEKIASMIEHWLVQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEVTNIFPRVAYCTARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHLFLHSHNV-SKSCEVEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKLFEKYQDKYQ-DQDFMDG 1396
            K  +D VD++S++ +  LL   S++V+ ++YV   I C+M     G    +Y E YC+++ T  + E S+IP   +D    +++ I YYQWVP +L  QA +F+LP  +W+ +     GL+  +++  +E    +   +  +  + +  ++    +       Y    C    K   +  S  Y+ +K +YL N   Q  ++  FL    +E +   +G     ++  G+ W+ + +FPRV  C    R +  V+  T QCVL  N+F EKIY+F+WFW V V I T  + L  ++RL   S  +N ++  L    +N   +   +  F +  L+ DG+ L+  I+ ++G + T  I  +LF+K+ +  Q ++DF  G
Sbjct:   18 KTFDDAVDRLSYVTTATLLAFFSIMVSCKQYVGSAIQCWMPMEFKG-GWEQYAEDYCFIQNTFFIPERSEIPGDVEDR---QKAEIGYYQWVPIVLAIQAFMFYLPSWIWSSL-YKQCGLDFPSVISEAEALRSQDSETRTKG-VNKLVDFIGDILDTRSKNEYGRFYCYRFGKGLGSMTSMLYICIKLMYLANVFVQFIILNKFL---GNETFL--WGFHTFADLYAGREWQDSGVFPRVTLCDFSVRKLANVHRYTVQCVLMINMFNEKIYLFIWFWFVFVLITTFINTLCTIYRLSFDSSRHNYIRSLLSGPVNNFKDEKAMIASFANNGLKQDGVLLMRFIDDHAGAMVTKEICEELFKKHGENLQHNRDFHHG 384          
BLAST of SMED30032157 vs. TrEMBL
Match: G7YJJ0 (Innexin OS=Clonorchis sinensis OX=79923 GN=inx PE=3 SV=1)

HSP 1 Score: 337.806 bits (865), Expect = 2.260e-104
Identity = 167/403 (41.44%), Postives = 259/403 (64.27%), Query Frame = -3
Query:  266 DVVDKISSLPVLNYKGLEDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYMSHTPGGENILKYLEHYCWVRGTMSLD-ESKIPQKSQDWHEFERSRIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLV----KISETTEPEK------IASMIEHWLVQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEVTNIFPRVAYCTARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHLFLHSHNVSKS-CEVEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKLFEKYQDKYQDQDFMDGDK 1438
            + +D IS   V  Y G+EDF DK +F+ + ++L+ C+ +VT+++Y+M PI+CYM+   GG+N+L Y+E+YCWV+GT+ +    K+P+    W E E+ +++YYQWVPF+L  Q  +F++P+++W +IC N TG ++++LV    +    T+ ++      +A  +E  L QHREYR     +++ RL++    LV+SKR  T L   YL +KCLYLVN++GQ+++MQ FL L  ++ YS  FG+ IA++I+ G++W+ T +FPRV YC    RH+G  N +T QCVL  N+  E+IY+FLWFWIVL A  T+ S+ TW  R+         +KK+L L      K    VEKF  +FLR DGIFLL MI +N+GDL   +IV +L++ ++ +Y  +D    D+
Sbjct:    5 EFLDYISKFQVATYVGVEDFADKFNFLVTVMILLLCTTVVTVKQYMMKPISCYMATDIGGKNLLDYVENYCWVQGTIPIAYAGKMPETDAAWEEMEKHKLLYYQWVPFVLGLQCIMFYVPRVIWQMICYNRTGTDIQHLVLSANQAVHATDDQRTKMIQHVARTLEQMLFQHREYRNDVWSHIRRRLWKTCSLLVVSKRLGTRLFAIYLFIKCLYLVNAVGQVFMMQTFLGLR-YDNYSF-FGIAIARDILSGKDWQRTLVFPRVGYCLVPVRHMGASNYVTGQCVLPVNMLNERIYVFLWFWIVLAATLTAISIPTWFMRMSYQKSRTCFIKKYLKLGEVLTKKDRGMVEKFKRQFLRQDGIFLLRMIAINAGDLICSDIVCQLWKIFKTRYFFRDLTMSDE 405          
BLAST of SMED30032157 vs. TrEMBL
Match: A0A4S2LNY2 (Innexin OS=Opisthorchis felineus OX=147828 GN=inx PE=3 SV=1)

HSP 1 Score: 337.421 bits (864), Expect = 3.422e-104
Identity = 167/403 (41.44%), Postives = 259/403 (64.27%), Query Frame = -3
Query:  266 DVVDKISSLPVLNYKGLEDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYMSHTPGGENILKYLEHYCWVRGTMSLD-ESKIPQKSQDWHEFERSRIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLV----KISETTEPEK------IASMIEHWLVQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEVTNIFPRVAYCTARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHLFLHSHNVSKS-CEVEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKLFEKYQDKYQDQDFMDGDK 1438
            + +D IS   V  Y G+EDF DK +F+ + ++L+ C+ +VT+++Y+M PI+CYM+   GG+N+L Y+E+YCWV+GT+ +    K+P+    W E E+ +++YYQWVPF+L  Q  +F++P+++W +IC N TG ++++LV    +    T+ ++      +A  +E  L QHREYR     +++ RL++    LV+SKR  T L   YL +KCLYLVN++GQ+++MQ FL L  ++ YS  FG+ IA++I+ G++W+ T +FPRV YC    RH+G  N +T QCVL  N+  E+IY+FLWFWIVL A  T+ S+ TW  R+         +KK+L L      K    VEKF  +FLR DGIFLL MI +N+GDL   +IV +L++ ++ +Y  +D    D+
Sbjct:    5 EFLDYISKFQVATYVGVEDFADKFNFLITVMILLLCTTVVTVKQYMMKPISCYMATDIGGKNLLDYVENYCWVQGTIPIAYAGKMPETDAAWEEMEKHKLLYYQWVPFVLGLQCIMFYVPRVIWQMICYNRTGTDIQHLVLSANQAVHATDDQRTKMIQHVARTLEQMLFQHREYRNDVWSHIRRRLWKTCSLLVVSKRLGTRLFAIYLFIKCLYLVNAVGQVFMMQTFLGLR-YDNYSF-FGIAIARDILSGKDWQRTLVFPRVGYCLVPVRHMGASNYVTGQCVLPVNMLNERIYVFLWFWIVLAATLTAISIPTWFMRMSYQKSRTCFIKKYLKLGEVLTKKDRGMVEKFKRQFLRQDGIFLLRMIAINAGDLICSDIVCQLWKIFKTRYFFRDLTMSDE 405          
BLAST of SMED30032157 vs. TrEMBL
Match: Q2L6M7 (Innexin OS=Dugesia japonica OX=6161 GN=inx8 PE=2 SV=1)

HSP 1 Score: 332.028 bits (850), Expect = 2.199e-102
Identity = 172/411 (41.85%), Postives = 252/411 (61.31%), Query Frame = -3
Query:  233 ISSLPVLNYKGLEDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYMSHTPGGENILKYLEHYCWVRGTMS-LDESKIPQKSQDWHEFERS-RIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLVKISE---TTEPE-------KIASMIEHWLVQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEVTNIFPRVAYC-TARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHLFLH-SHNVSKSCEVEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKLFEKYQDKYQDQDFMDGDKFLLCKTGKNSE 1423
            +S   V +Y G+EDF DKM+F+ S +L+I   ++VT++ Y   P++CY++ TP G     Y+E+YCWV GT+  L    IPQK+ +W E++ + RI YYQWVPF+L  Q  +F+ PKI+W +IC N  G N++NLV  +E    + PE       +I+  IE  L QHR+YR+G + N +  LY     LV SK   TWL   Y  +K LY +N IGQ+YLM+ FL  +N   Y   FG  I +N++ G+ W  T IFPRV+YC  A  RH+G  N   +QC L  N+  EKIY+FLWFW++LV I T  S+++W+ ++   S+  + +KK L +H S+  +    V +F  E+LR DG+FL+ MI +N+GD+ T  I+  L+E Y++KY D DF + + +   K   NSE
Sbjct:   10 LSEFKVSSYVGVEDFADKMNFMFSVVLIILSMMVVTVKSYFFKPLSCYIATTPSGSGFDNYIENYCWVHGTIPILPGENIPQKTNEWAEWDANKRITYYQWVPFILGLQCIMFYTPKIIWQIICYNKIGTNLENLVNGAEEASKSPPEDRKALLDRISRTIEDMLYQHRDYRQGKIANTRRALYSRCNFLVFSKHLGTWLVLSYFFIKVLYGINVIGQLYLMKSFLGFDNSLTY---FGYTILENMLNGKEWHQTGIFPRVSYCYNADIRHLGSTNAYVSQCTLPINMLNEKIYVFLWFWVLLVGIITLISIISWLIKMVFLSKRSSFIKKLLKMHQSYKRTDQLLVNQFIKEYLRHDGVFLIRMICINAGDIVTAEILGTLWEIYKEKYIDSDFRNFNNY---KKLNNSE 414          
BLAST of SMED30032157 vs. TrEMBL
Match: A0A4E0RLV9 (Innexin OS=Fasciola hepatica OX=6192 GN=inx PE=3 SV=1)

HSP 1 Score: 329.717 bits (844), Expect = 2.011e-101
Identity = 167/392 (42.60%), Postives = 250/392 (63.78%), Query Frame = -3
Query:  296 DKISSLPVLNYKGLEDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYMSHTPGGENILKYLEHYCWVRGTMS-LDESKIPQKSQDWHEFERS-RIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLVKI----SETTEPEK------IASMIEHWLVQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEVTNIFPRVAYC-TARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHLFLHSH-NVSKSCEVEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKLFEKYQDKY 1429
            D +    + +Y G+EDF DK+SF+ S +++  C+L+VT+++Y+  PI CY+   P G +  K+LE +CWV+GT+  L   KIPQ   +W  F+R  RI YYQW+PFML  Q  +F++P+I+W +IC + TG ++++LV +    S++   E+      +AS +E  L QHREYR G   N+K R+Y++   LV+SKR  TWL+  Y ++K  YL NS+GQ+Y+MQ FL  N   E   NFG ++A  +++G+NWE T IFPR+++C  A  R +G VN   AQCVL  N+  EK+YIFLW+W  LVAI T+ S+  W+ RL +       +KK L +H     +    ++ F  E+LR DGIFLL MI++NSGD+ T  +VS+L++ Y  K+
Sbjct:    8 DYVFKFHIPDYVGVEDFADKLSFLYSVLIMFICTLVVTVKQYLFSPIACYIPTVPSGTDFDKFLESFCWVQGTIPILAGDKIPQTFDEWAYFDRKYRINYYQWIPFMLGLQGMMFYIPRIMWQMICSHRTGTDLEHLVTVANQASQSGPDERKKLVTHVASSMEAMLYQHREYRTGKCTNIKQRVYKVCGLLVVSKRLGTWLAFSYFIIKLCYLANSVGQLYMMQRFLGFN---ETMGNFGAQLASYMLEGRNWEQTRIFPRISFCYLADIRQLGSVNRYVAQCVLPVNMLNEKLYIFLWYWTSLVAICTAVSIPLWVIRLGILRNRVYFIKKFLRIHEQFQKADKLLLKSFTREYLRHDGIFLLRMISMNSGDVITSEVVSELWKMYCAKH 396          
BLAST of SMED30032157 vs. TrEMBL
Match: W6UMB0 (Innexin OS=Echinococcus granulosus OX=6210 GN=inx PE=3 SV=1)

HSP 1 Score: 330.872 bits (847), Expect = 4.260e-101
Identity = 164/412 (39.81%), Postives = 258/412 (62.62%), Query Frame = -3
Query:  275 RIVILPMGTFDVVDKISSLPVLNYKGLEDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYMSHTPGGENILKYLEHYCWVRGTMSLDESK-IPQKSQDWHEF-ERSRIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLV----KISETTEPEK------IASMIEHWLVQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEVTNIFPRVAYC-TARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHLFLHSHNVSKSCEV-EKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKLFEKYQDKYQDQDFMD 1468
            R     M   + +D ++ L V ++ GLEDF DKMSF+ S ++L+ C+ I+ +++Y++  I CY+   P G++  K+L+++CWV GT+ + E + IPQ   +WH+  E  RI YYQWVPFML  Q  LF++P+++W +IC N TG +++NL+    K S T E ++      +A  +   L QHR+YR+G V + + + ++     + SKR  TWL   YL +KC+YL N+IGQ+YLMQ FL  N      SNFG E+A+ ++ G++W+ T IFPRV++C     RH+G  N   AQCVL  N+  EK+YIFLWFW  +VA+ T+ S+  W+ RL       + +KK L L      +  E+   F S+FLR DG+FLL MI++N+GD+ T ++V ++++ Y+ KY+++  ++
Sbjct:   55 RSTAAKMVGIEFIDYLTKLHVSDFLGLEDFADKMSFMYSVLILLLCTTIIAVKQYLLASIACYIPTVPSGKDFEKFLQNFCWVHGTIPILEGEHIPQNYSEWHDADENMRINYYQWVPFMLGLQCILFYVPRVVWQIICYNRTGTDLENLIAVANKASNTIENDRKMLVKHVAGTMAEMLFQHRDYRRGKVADARRKAFKFFGMFIASKRLGTWLIFTYLFIKCIYLANAIGQLYLMQTFLGFNKS---MSNFGYEVARYMVNGRDWDETRIFPRVSFCYLEHVRHLGTTNRYVAQCVLPVNMLNEKLYIFLWFWTAIVAVCTAVSIPLWIMRLGREKNKVHFIKKFLRLQDIYTPEHKEILRNFISDFLRHDGVFLLRMISMNAGDVVTADVVVEMWDIYKTKYKNRAMLN 463          
BLAST of SMED30032157 vs. Planmine SMEST
Match: SMESG000032745.1 (SMESG000032745.1)

HSP 1 Score: 856.284 bits (2211), Expect = 0.000e+0
Identity = 412/412 (100.00%), Postives = 412/412 (100.00%), Query Frame = -3
Query:  215 MGTFDVVDKISSLPVLNYKGLEDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYMSHTPGGENILKYLEHYCWVRGTMSLDESKIPQKSQDWHEFERSRIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLVKISETTEPEKIASMIEHWLVQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEVTNIFPRVAYCTARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHLFLHSHNVSKSCEVEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKLFEKYQDKYQDQDFMDGDKFLLCKTGKNSELSTFVS 1450
            MGTFDVVDKISSLPVLNYKGLEDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYMSHTPGGENILKYLEHYCWVRGTMSLDESKIPQKSQDWHEFERSRIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLVKISETTEPEKIASMIEHWLVQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEVTNIFPRVAYCTARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHLFLHSHNVSKSCEVEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKLFEKYQDKYQDQDFMDGDKFLLCKTGKNSELSTFVS
Sbjct:    1 MGTFDVVDKISSLPVLNYKGLEDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYMSHTPGGENILKYLEHYCWVRGTMSLDESKIPQKSQDWHEFERSRIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLVKISETTEPEKIASMIEHWLVQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEVTNIFPRVAYCTARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHLFLHSHNVSKSCEVEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKLFEKYQDKYQDQDFMDGDKFLLCKTGKNSELSTFVS 412          
BLAST of SMED30032157 vs. Planmine SMEST
Match: SMESG000080788.1 (SMESG000080788.1)

HSP 1 Score: 327.02 bits (837), Expect = 6.462e-104
Identity = 171/419 (40.81%), Postives = 249/419 (59.43%), Query Frame = -3
Query:  224 ISSLPVLNYKGLEDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYMSHTPGGENILKYLEHYCWVRGTMS-LDESKIPQKSQDWHEFERS-RIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLV---KISETTEP-------EKIASMIEHWLVQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEVTNIFPRVAYCTAR-FRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHLFLH-SHNVSKSCEVEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKLFEKYQDKYQDQDFMDGDKF--LLCKTG---KNSELST 1423
            I    +  Y G+EDF DK+SF+ S  LLI   ++VT++ Y+  P+ CY +  PGG N   YLE+YCWV GT++ L    IPQ   DW   + + RI YYQWVPF+L  Q  +F++PKI+W +IC N +G N++++V     +  T P       E+IA  IE  L QHR+YR+G    ++ ++Y      V SK   TWL   Y+++K LY +N IGQ+++M+ FL  NN    +S+FG  +  N+I G+ W  T IFPRV YC  R  +HVG  N   AQC L  N+  EKIY+FLWFW+VLV I T+ S+  W +R+   SR    +K+ L +H ++  S    +  F  EFLR DG+FL+ M+ +N+GD+ T +IV++L E Y++ Y+  DF +   +   L K+G   +N  LS 
Sbjct:   10 IGQFKISTYVGIEDFADKLSFLFSVTLLILSMMVVTIKSYLFKPLACYTATAPGGSNFENYLENYCWVHGTITFLPGEDIPQNDVDWARADDTRRINYYQWVPFILGLQCFMFYIPKIIWQIICYNKSGTNLESIVNDANAANNTPPVERKDKIERIARTIEDMLFQHRDYRQGNFAKLRRKIYNTCNFCVFSKHMGTWLILSYILIKFLYGLNVIGQLFIMKKFLGFNNS---TSSFGYTVLSNMISGKEWHQTGIFPRVTYCYVRNIKHVGVANKYVAQCALPVNMLNEKIYVFLWFWMVLVGIVTAISIPLWFYRMAFLSRRSKFIKRFLMMHQNYERSDKPMINNFIEEFLRHDGVFLVRMLFINAGDIITADIVNRLLEIYKENYKHLDFRNYTAYTKALDKSGDLIRNKNLSA 425          
BLAST of SMED30032157 vs. Planmine SMEST
Match: SMESG000065083.1 (SMESG000065083.1)

HSP 1 Score: 300.442 bits (768), Expect = 9.263e-94
Identity = 154/409 (37.65%), Postives = 251/409 (61.37%), Query Frame = -3
Query:  272 MGTFDVVDKISSLPVLNYKGLEDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYMSHTPGGENILKYLEHYCWVRGTMSL-DESKIPQKSQDWHEFERSRIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLV-----KISETTEPEK-----IASMIEHWLVQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEVTNIFPRVAYCTARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHLFLHSHNVSKSCE----VEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKLFEKYQDKYQDQDFM-DG 1450
            M   + +D +S L V    GLED  D+++F  S I+L+ C LIVT+++YV  PI+CY+ +  GG N+L Y+E+ CWV+GT++L +++K P   ++W   E+ +++YYQWVPFML  Q  +F++PK++W +IC N TG ++++LV      +  + E        ++  +E  L QHR++R G +   + +++  +  L+ SKR  TWL   YL++K  Y+ N   Q+ +MQ FL  ++    +  FG+E+  NI  G +W+ T IFPRV+YC  +  H+G +N + AQCVL  N+  E+IY FLW WI++V   T  S+  W+ R+       + +++ L + S  ++K  +    + KF S+FLR DGIFLL MI+LN+GD+ T  IV +L+  Y+  Y +++F+ DG
Sbjct:    1 MVASEFLDYLSKLQVSTNVGLEDLPDRLNFTFSVIVLMVCILIVTVKQYVFKPISCYLPNNIGGSNLLSYVENLCWVQGTIALSNQAKFPSTDEEWKNLEKHKMLYYQWVPFMLGLQCIMFYVPKLVWQIICYNRTGTDIQHLVVSANQAVHSSDEARSKMVSYLSKTLEQLLYQHRDHRSGKLAKFRRKIHNSVGFLMCSKRIGTWLVSSYLLLKLSYVANGFIQLIIMQKFLGFSDFNLLT--FGIEVGNNIWSGTDWKTTLIFPRVSYCFVKVTHLGVINSMVAQCVLPVNMLNERIYFFLWCWILIVLSLTILSIPIWILRILCIKYRRHFIRQFLII-SEEITKDQKENYLLAKFESQFLRHDGIFLLKMISLNAGDIITSEIVCQLWNIYKTNYVNRNFLNDG 406          
BLAST of SMED30032157 vs. Planmine SMEST
Match: SMESG000070656.1 (SMESG000070656.1)

HSP 1 Score: 281.567 bits (719), Expect = 5.517e-87
Identity = 148/364 (40.66%), Postives = 224/364 (61.54%), Query Frame = -3
Query:  269 MREYVMDPITCYMSHTPGGENILKYLEHYCWVRGTMSLD-ESKIPQKSQDWHEFERSRIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLV-----KISETTEPEK-----IASMIEHWLVQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEVTNIFPRVAYCTARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHLFLHS---HNVSKSCEVEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKLFEKYQDKYQDQDFM-DGD 1315
            M++Y++ PI+CY+S T  G N+L+Y+E+YCWV GT+ +  + KIP  +++W E  + +I+YYQWVPF+L  QA +F++P+++W + C N TG ++ +L+      +  + E        IA  IE  L QHR+ R G +   +  LY+I    ++SKR  T L C Y ++K LYL N I Q+Y M+ FL LN   E    FG +I KNII GQ+W+ T +FPRV YC    ++VG  N L AQCVL  N+  EKI+ FLW WI   AI  + S++ W+FR  V  R    + + L L+    +   + C V KF  +FLR DG+FLL MI+LN+GD+   +IV +L++ ++ +Y +++F+ DG 
Sbjct:    1 MKQYILKPISCYIS-TISGTNLLEYVENYCWVEGTIPISYDGKIPSTNEEWAELNKYKILYYQWVPFLLSLQAIMFYIPRLIWQIFCYNTTGTDIHHLIVSANQAVHSSDEARTKMVNYIAKTIEQMLYQHRDNRNGRLARFRRSLYKICCFCIMSKRQGTRLLCMYFIIKFLYLTNCILQLYFMKCFLGLN---EAGIFFGFKIMKNIINGQDWKSTLVFPRVGYCYVNVKNVGTTNALVAQCVLPVNMLNEKIFFFLWCWIFSSAILLTISIIIWIFRTSVYQRRRGFIYRFLKLNQQLKNCDEEKCMVVKFEHQFLRYDGVFLLKMISLNAGDIVAADIVCQLWKIFKSQYANRNFLSDGS 360          
BLAST of SMED30032157 vs. Planmine SMEST
Match: SMESG000053975.1 (SMESG000053975.1)

HSP 1 Score: 276.944 bits (707), Expect = 6.074e-85
Identity = 161/423 (38.06%), Postives = 239/423 (56.50%), Query Frame = -3
Query:  215 FDVVDKISSLPVLNYKGLEDFVDKMSFIASCILLIACSLIVTMREYVMDPITCYMSHTPGGENILKYLEHYCWVRGTMSL-DESKIPQKSQDWHEFERS-RIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLVKIS----------ETTEPEKIASMIEHWLVQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCGYLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWEVTNIFPRVAYC-TARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLVAIFTSFSLLTWMFRLFVPSRHYNEVKKHL-FLHSHNVSKSCEVEKFCSEFLRLDGIFLLYMINLNSGDLATGNIVSKLFEKYQDKYQDQDFMDGDKFLLCKTGKNSELSTFVS 1441
            F +++++++  V NY GLED  DKMS I S ++L  C  IVT++ YV+ PI+CY+S  P G N   Y+E+YCW+ GT+ L    K+      W E ER+ +I YYQWVPF+L  Q  +F++PK++W + C N  G N+  ++K+S             E E IA +IE+ LV HR++R   + N++  +Y      + SKR  T +   Y + K LYL N IGQ++++Q  L   N   Y   FG  +   I+ G   E  + FPRV +C   R RHVG  N   AQCVL  N+  EKIY FLW WI+LV+  T  S+  W+ RL+      + +   L  L+S++  K   + KF ++FLR DG+FLL MI+ N+GDL    +V+ L++ Y  KYQ+++ +  D F +    K  E +   S
Sbjct:    7 FSLINRLNA--VHNY-GLEDLADKMSSIFSVLILSVCVSIVTIKSYVIKPISCYLSIGPSGGNFQDYIENYCWIHGTIPLYPNQKLSDNHVGWDELERTQKINYYQWVPFILGLQCIMFYIPKLIWQIFCFNQAGTNLDTIIKLSMKMAEIPISERRVENENIAKIIENMLVLHRDFRDSKIANIRRSVYSKCSLFMSSKRLGTMIVFSYFVTKLLYLTNGIGQLFMIQSVLGFYN---YLPTFGSHLFWYIVSGTELETQHYFPRVVFCYLKRIRHVGVQNNYVAQCVLPVNMLNEKIYTFLWCWILLVSFATFISIPLWINRLYRADVREHYIGNFLQMLNSYDEHKKPLIHKFINDFLRHDGLFLLRMISNNAGDLIAAYLVNNLWDIYLKKYQNRN-LRKDIFSVSNHLKEDEANNSTS 422          
The following BLAST results are available for this feature:
BLAST of SMED30032157 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30032157 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
unc-97.312e-5531.97Innexin unc-9 [Source:UniProtKB/Swiss-Prot;Acc:O0... [more]
inx-18.886e-5530.87Innexin [Source:UniProtKB/TrEMBL;Acc:Q17394][more]
inx-18.886e-5530.87Innexin [Source:UniProtKB/TrEMBL;Acc:Q17394][more]
inx-15.387e-5431.09Innexin [Source:UniProtKB/TrEMBL;Acc:Q17394][more]
inx-112.823e-5329.86Innexin-11 [Source:UniProtKB/Swiss-Prot;Acc:Q2315... [more]
back to top
BLAST of SMED30032157 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Inx26.817e-2927.76gene:FBgn0027108 transcript:FBtr0301860[more]
Inx26.817e-2927.76gene:FBgn0027108 transcript:FBtr0071005[more]
Inx26.817e-2927.76gene:FBgn0027108 transcript:FBtr0332314[more]
Inx26.817e-2927.76gene:FBgn0027108 transcript:FBtr0071006[more]
shakB4.662e-2027.27gene:FBgn0085387 transcript:FBtr0112571[more]
back to top
BLAST of SMED30032157 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30032157 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30032157 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30032157 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|O01393|UNC9_CAEEL9.302e-5431.97Innexin unc-9 OS=Caenorhabditis elegans OX=6239 GN... [more]
sp|Q23157|INX11_CAEEL3.591e-5229.86Innexin-11 OS=Caenorhabditis elegans OX=6239 GN=in... [more]
sp|Q03412|UNC7_CAEEL5.455e-4829.44Innexin unc-7 OS=Caenorhabditis elegans OX=6239 GN... [more]
sp|A8WVX4|INX19_CAEBR1.073e-4226.76Innexin-19 OS=Caenorhabditis briggsae OX=6238 GN=i... [more]
sp|Q19746|INX3_CAEEL2.672e-4228.31Innexin-3 OS=Caenorhabditis elegans OX=6239 GN=inx... [more]
back to top
BLAST of SMED30032157 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
G7YJJ02.260e-10441.44Innexin OS=Clonorchis sinensis OX=79923 GN=inx PE=... [more]
A0A4S2LNY23.422e-10441.44Innexin OS=Opisthorchis felineus OX=147828 GN=inx ... [more]
Q2L6M72.199e-10241.85Innexin OS=Dugesia japonica OX=6161 GN=inx8 PE=2 S... [more]
A0A4E0RLV92.011e-10142.60Innexin OS=Fasciola hepatica OX=6192 GN=inx PE=3 S... [more]
W6UMB04.260e-10139.81Innexin OS=Echinococcus granulosus OX=6210 GN=inx ... [more]
back to top
BLAST of SMED30032157 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30032157 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30032157 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30032157 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30032157 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30032157 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000032745.10.000e+0100.00SMESG000032745.1[more]
SMESG000080788.16.462e-10440.81SMESG000080788.1[more]
SMESG000065083.19.263e-9437.65SMESG000065083.1[more]
SMESG000070656.15.517e-8740.66SMESG000070656.1[more]
SMESG000053975.16.074e-8538.06SMESG000053975.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30032157 ID=SMED30032157|Name=SMED30032157|organism=Schmidtea mediterranea sexual|type=transcript|length=2334bp
TAATAATAATAACAATAATAATACAAAACATTTGAAACTCTTCTTAACCG
TTTAAAAGGTCAGTAAATAAATGATGTCACATGAGAACAGAAATTTAGCG
ATGCATTTATCTACAAGTGAGGTGGTTCCTGGGGGGGTCACACAAATGAA
CTGAGGCATAAATCACAGTGAATACACTATTTGACATTCTCTAGTTTTCC
CTTCTGGCTTTTCAGCTTACGAACGTCGACAATTCCGAATTTTTGCCTGT
TTTACACAATAGGAATTTGTCGCCATCCATGAAATCCTGATCTTGGTACT
TATCTTGATACTTCTCGAACAGCTTCGACACGATATTTCCCGTCGCCAAG
TCCCCGGAATTCAGATTGATCATGTAGAGAAGGAAGATTCCGTCTAATCT
AAGGAATTCTGAGCAGAATTTCTCGACTTCACAAGATTTGCTGACATTAT
GACTGTGAAGAAATAAATGTTTCTTGACTTCATTGTAATGCCTGGAAGGA
ACAAAGAGCCGGAACATCCAAGTAAGTAATGAAAAAGAAGTGAATATGGC
CACTAGGACAATCCAGAACCAGAGGAAAATATAAATTTTCTCAATGAAAA
GATTGGCCGTCAGAACGCATTGGGCAGTAAGTAAGTTCACACCGCCCACG
TGCCTAAACCGAGCCGTGCAATATGCGACTCTGGGGAATATATTGGTGAC
TTCCCAGTTTTGGCCCTTGATGATGTTTTTTGCTATTTCCAAGCCAAAAT
TTGAGCTGTACTCCTCGTGATTATTTAGTTGGAGAAAATGCTGCATGAGA
TATATCTGGCCGATGCTATTCACCAGGTAAAGGCACTTCATCATCAGGTA
GCCACAACTGAGCCAAGTCGCAAAACGTTTGCTGATAACCAAGCATTTCA
TTATCCGGTAGAGCCTGACTTTCATGTTGTGAACGAATCCCTTCCGGTAC
TCTCTGTGCTGGACCAACCAATGCTCAATCATTGAAGCTATTTTCTCGGG
TTCTGTTGTCTCTGAAATCTTGACCAGATTTTTGACATTCAAGCCTGTTA
CGTTGAGACATATCACAGTCCAGAGGATTTTCGGCAGATGGAAGAGGGCG
GCCTGAAACAGCAGCATGAAAGGCACCCATTGATAATACACAATTCTAGA
TCTTTCAAACTCATGCCAATCTTGACTTTTCTGAGGGATTTTTGATTCAT
CGAGGCTCATCGTTCCCCGGACCCAACAATAGTGCTCCAGATATTTAAGA
ATATTCTCTCCTCCTGGTGTATGGGACATGTAGCATGTGATCGGGTCCAT
TACGTACTCCCGCATCGTGACGATGAGGCTGCACGCAATCAGCAATATGC
AACTGGCTATGAAACTCATCTTGTCCACGAAGTCCTCCAGTCCTTTATAG
TTAAGAACTGGAAGACTTGAAATCTTGTCGACAACATCAAATGTACCCAT
CGGTAAAATGACAATGCGAAATTCTAACCGAATTATATACGTATGAATAT
GCAAATAACTAATGAGTATTCGACAGCTACGTGTGCCAACGCGTGTTCAC
AGTCCGTTGCGATAGGTTAAGTGGCTCAAGTACAGTTGTTGAAGTTGATA
GTAAGATATCGGGTCAAGTACGCATTTCAACACTGTGGGAAATCTCTCGC
ACTCAGGGTCACAACAAATAGCATGGTTACCATGTGAACTCCTTCCATCG
GTCAGGGCCTCATAACCTTTTGCTTGGACAGTCACTTGCGGTGGCAGCCA
TTCGGATTCACCAGCTCCGATAGATGCAACATTATGCCCTGTTATCAAAT
GGTCAGATAGAGCTGCAGGTCTCGTCTGAAAAAGTTTTATTGCGGGGAAG
CGAGGGGGGGGGTTATGAGGCCAGATGAATATACCGCTCTAAATACCGCG
AGTTTTTCTGGAGAGGAATGAAGGGGTGGCTGTAGCAACTAGTTGGTGAG
ACTAGGCCTCGTCAAATACAAAACATGAAGACTGCTAGTGTAAAACACGT
GATTAATTGTGAAATGAAAATAATAACAAAATCAGATCAATTTGCACAGT
CGATTGATAGATCAAATCGGTAATTTGATCGGCAAATGTTACAACAGAAT
AACAATTATGATCCAAGCTTCGAATAACATTTTCGCTAGGAAGAATGCAA
CAACAATAATAAATAAGACAATTCTGAGGGATGTAATAAGATGAAATGGA
CCACAACGAGCTGCAAGTGGGGTGCAATTGTGGTGCAATGGGATGCATAG
TATATAATGGACTGCAATGGACTGCAATGGGATGGCATGGTATGTAATGG
ACTGCAATGGACTGCAATGGGATGGCATGGTATG
back to top

protein sequence of SMED30032157-orf-1

>SMED30032157-orf-1 ID=SMED30032157-orf-1|Name=SMED30032157-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=413bp
MGTFDVVDKISSLPVLNYKGLEDFVDKMSFIASCILLIACSLIVTMREYV
MDPITCYMSHTPGGENILKYLEHYCWVRGTMSLDESKIPQKSQDWHEFER
SRIVYYQWVPFMLLFQAALFHLPKILWTVICLNVTGLNVKNLVKISETTE
PEKIASMIEHWLVQHREYRKGFVHNMKVRLYRIMKCLVISKRFATWLSCG
YLMMKCLYLVNSIGQIYLMQHFLQLNNHEEYSSNFGLEIAKNIIKGQNWE
VTNIFPRVAYCTARFRHVGGVNLLTAQCVLTANLFIEKIYIFLWFWIVLV
AIFTSFSLLTWMFRLFVPSRHYNEVKKHLFLHSHNVSKSCEVEKFCSEFL
RLDGIFLLYMINLNSGDLATGNIVSKLFEKYQDKYQDQDFMDGDKFLLCK
TGKNSELSTFVS*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000026gut
Vocabulary: INTERPRO
TermDefinition
IPR000990Innexin
Vocabulary: cellular component
TermDefinition
GO:0005921gap junction
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000990InnexinPRINTSPR01262INNEXINcoord: 201..215
score: 44.8
coord: 278..294
score: 58.59
coord: 74..83
score: 41.6
coord: 254..263
score: 48.8
coord: 105..127
score: 58.78
coord: 45..56
score: 35.33
IPR000990InnexinPFAMPF00876Innexincoord: 22..381
e-value: 1.9E-92
score: 310.3
IPR000990InnexinPANTHERPTHR11893INNEXINcoord: 13..390
IPR000990InnexinPROSITEPS51013PANNEXINcoord: 17..387
score: 28.738
NoneNo IPR availableTMHMMTMhelixcoord: 28..50
NoneNo IPR availableTMHMMTMhelixcoord: 291..313