SMED30032138
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30032138 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Homology
BLAST of SMED30032138 vs. TrEMBL
Match: A0A267FSD8 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig025013g6 PE=4 SV=1) HSP 1 Score: 135.961 bits (341), Expect = 1.385e-32 Identity = 115/332 (34.64%), Postives = 171/332 (51.51%), Query Frame = -2 Query: 145 NYETDIITLVSYKIKITVSLFGFVLNLWAFSFFHRFEKQSHFCFMLKHLSLVELIFSLSGVVFECFYFTLVVLQR---PFPKVLV--NFIIYFVFNFMWLMYKSSLLSRNWVIVLIAWRRYQAIHRPXXXXXXXXXXXXXXXIFGIYAISLFLVTPRLFEITYSYSNETKNVI-ITPYLLVNSIYLSTYPILFFILISPVPVCVICFYSIFMLRSLRK----------PECHELETRSDHFQRQNPTXXXXXXXXXXXXNKXXXXXXXXXXXCEFPMCLAGIFNQISRESTGVQFQWFLTHFAGVANILTSCDSIFNFVIYFISFPFFKQSI 1092 N T ++ Y IK ++ G ++NL + F F+++S F M+ +LS E+ F+ G+ FE FY + P L F+IY V +W++YKSSL+ RNW+IVLIAWRRY+AI LSS++ S++ + + I ++ L TPR FE+ Y E++ ++ + P +S S YP LFF+L SP+P+ + S M SLR P + + + D + N T R + N+TVLVLC F CE PM +AGI ++ F+GVAN LTS DS NFV+Y +S P F+ + Sbjct: 34 NGPTQVMLDSLYFIKCIAAMIGLMVNLVSIKVFLSFKRRSLFISMIFYLSCCEVCFNSCGLSFELFYLLVGSTNSHLMPIGSSLAVAQFLIYLVCQVVWIIYKSSLILRNWIIVLIAWRRYRAISASLSSQRSQSRRSFRLSLLLIGLLAALLTTPRAFELAYFLCAESRQIVRLAPAAFAHSRRYSAYPKLFFVLTSPMPIVCLLVISTLMTCSLRAAANTANVLQVPISNSVSSTRDR-ESTNSTSMRHRRSQSDAVNRTVLVLCGSFVLCELPMLIAGIIKDFECGDHCMKVHV----FSGVANTLTSMDSFANFVVYVVSIPSFRNKL 360
BLAST of SMED30032138 vs. TrEMBL
Match: A0A1I8HVZ0 (Phosphodiesterase OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 87.8113 bits (216), Expect = 5.578e-15 Identity = 74/232 (31.90%), Postives = 111/232 (47.84%), Query Frame = -2 Query: 145 YFVFNFMWLMYKSSLLSRNWVIVLIAWRRYQAIHRPXXXXXXXXXXXXXXXIFGIYAI-SLFLVTPRLFEITYSYSNETKNVI-ITPYLLVNSIYLSTYPILFFILISPVPVCVICFYSIFMLRSLRKPECHELETRSDHFQRQNPTXXXXXXXXXXXXNKXXXXXXXXXXXCEFPMCLAGIFNQISRESTGVQFQWFLTHFAGVANILTSCDSIFNFVIYFISFPFFKQSI 834 Y + +W++YK+SL+ RNW+IV+IAWRRY+AI L+ + + L + + L PR E+ Y E++ ++ + +S S YP +FF+ SP+P+ + S ML SLR T + + S N+TV VLC F CE PM +AG+ Q F+ G+ANILTS DS+ NF +YF S F+ + Sbjct: 1 YLLCQAVWVVYKASLILRNWIIVVIAWRRYRAIRASLNGYRSGPGRRSLRLSLLCLGVLAALLTAPRSLEVAYYLCVESRQLVRVAVAGFASSRGYSVYPKIFFVATSPLPIVCLLVISFLMLCSLRN------STDAQLLHGGGGSGGSGSGGRCDSVNRTVQVLCATFVLCELPMLVAGVVKDADCNEHCPHLQVFM----GIANILTSLDSLANFGVYFFSIRAFRHKL 222
BLAST of SMED30032138 vs. TrEMBL
Match: A0A3S5ANT6 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS27551 PE=4 SV=1) HSP 1 Score: 77.411 bits (189), Expect = 1.271e-11 Identity = 40/85 (47.06%), Postives = 51/85 (60.00%), Query Frame = -2 Query: 727 FCFMLKHLSLVELIFSLSGVVFECFYFTLVVLQRPFPKVLVNFIIYF----VFNFMWLMYKSSLLSRNWVIVLIAWRRYQAIHRP 969 F ML HLS VE++ LSG +FE YF+ +VL P L + YF V W++YK+SLL+RNWVI IAWRR +H P Sbjct: 246 FSLMLCHLSAVEMVCGLSGFLFETVYFSTIVLGDESPVFLSPRLAYFLALGVLQLDWIVYKASLLARNWVIAFIAWRRLCVVHDP 330
BLAST of SMED30032138 vs. Planmine SMEST
Match: SMESG000013334.1 (SMESG000013334.1) HSP 1 Score: 650.203 bits (1676), Expect = 0.000e+0 Identity = 368/369 (99.73%), Postives = 368/369 (99.73%), Query Frame = -2 Query: 25 MFNFTINESSKGINYETDIITLVSYKIKITVSLFGFVLNLWAFSFFHRFEKQSHFCFMLKHLSLVELIFSLSGVVFECFYFTLVVLQRPFPKVLVNFIIYFVFNFMWLMYKSSLLSRNWVIVLIAWRRYQAIHRPXXXXXXXXXXXXXXXIFGIYAISLFLVTPRLFEITYSYSNETKNVIITPYLLVNSIYLSTYPILFFILISPVPVCVICFYSIFMLRSLRKPECHELETRSDHFQRQNPTXXXXXXXXXXXXNKXXXXXXXXXXXCEFPMCLAGIFNQISRESTGVQFQWFLTHFAGVANILTSCDSIFNFVIYFISFPFFKQSILKGFSAISCYDFQNNYRLDNSNFSRQKTINQCEETVFSND 1131 MFNFTINESSKGINYETDIITLVSYKIKITVSLFGFVLNLWAFSFFHRFEKQSHFCFMLKHLSLVELIFSLSGVVFECFYFTLVVLQRPFPKVLVNFIIY VFNFMWLMYKSSLLSRNWVIVLIAWRRYQAIHRPLSSKQLLSKKLIQYLIFGIYAISLFLVTPRLFEITYSYSNETKNVIITPYLLVNSIYLSTYPILFFILISPVPVCVICFYSIFMLRSLRKPECHELETRSDHFQRQNPTISRRIARNRSSSNKTVLVLCTVFTTCEFPMCLAGIFNQISRESTGVQFQWFLTHFAGVANILTSCDSIFNFVIYFISFPFFKQSILKGFSAISCYDFQNNYRLDNSNFSRQKTINQCEETVFSND Sbjct: 1 MFNFTINESSKGINYETDIITLVSYKIKITVSLFGFVLNLWAFSFFHRFEKQSHFCFMLKHLSLVELIFSLSGVVFECFYFTLVVLQRPFPKVLVNFIIYLVFNFMWLMYKSSLLSRNWVIVLIAWRRYQAIHRPLSSKQLLSKKLIQYLIFGIYAISLFLVTPRLFEITYSYSNETKNVIITPYLLVNSIYLSTYPILFFILISPVPVCVICFYSIFMLRSLRKPECHELETRSDHFQRQNPTISRRIARNRSSSNKTVLVLCTVFTTCEFPMCLAGIFNQISRESTGVQFQWFLTHFAGVANILTSCDSIFNFVIYFISFPFFKQSILKGFSAISCYDFQNNYRLDNSNFSRQKTINQCEETVFSND 369
BLAST of SMED30032138 vs. Planmine SMEST
Match: SMESG000061638.1 (SMESG000061638.1) HSP 1 Score: 133.265 bits (334), Expect = 1.841e-35 Identity = 115/314 (36.62%), Postives = 173/314 (55.10%), Query Frame = -2 Query: 145 IKITVSLFGFVLNLWAFSFFHRFEKQSHFCFMLKHLSLVELIFSLSGVVFECFYFTLVVLQRPFPKVL-VNFIIYFVFNFMWLMYKSSLLSRNWVIVLIAWRRYQAIHRPXXXXXXXXXXXXXXXIFGIYAISLFLVTPRLFEITYSYSNETKNVIITPYLLVNSIYLST-----YPILFFILISPVPVCVICFYSIFMLRSLRKPECH-----ELETRSDHFQRQNPTXXXXXXXXXXXXNKXXXXXXXXXXXCEFPMCLAGIFNQISRESTGVQFQWFLTHFAGVANILTSCDSIFNFVIYFISFPFFKQSI 1053 +K T SL G + N+ + +F + F +L HLS VE+IF SG +FE Y+ FIIY V+ ++W++YKSSLL+RNW+ ++AW+RY+AI PLS+KQ + K I ++F +Y + + PR FE +Y Y E IIT + NSIY + Y L+F L SP+P+ VI S+ ML L+K + + +R +++++ + S NKTV++LC FT CE P C+AGI + ST + L++F G+A +LTS DS+ +F IYF S F+Q+I Sbjct: 1 MKATFSLLGLMFNIISMIYFATVNRYKIFNLLLLHLSCVEVIFCSSGFIFEVTYYWYAAKHNILSYTWQAKFIIYLVYFWLWVVYKSSLLARNWITAVVAWQRYRAISHPLSTKQYETYKSISMILFIMYFLVILFSIPRAFEKSY-YLCEKDVYIIT---MNNSIYYTDLYKNIYNPLYFFLSSPLPLLVIIVCSLSMLIVLKKRQENLNNLTSQPSRFSKYKQKSSLGNNSSNSRGQSINKTVIILCAAFTICELPACIAGIMLNV---STDMSIWKILSYFVGIAMVLTSVDSLLDFFIYFYSIVHFRQAI 307
BLAST of SMED30032138 vs. Planmine SMEST
Match: SMESG000063294.1 (SMESG000063294.1) HSP 1 Score: 62.7734 bits (151), Expect = 1.117e-10 Identity = 95/339 (28.02%), Postives = 153/339 (45.13%), Query Frame = -2 Query: 118 ETDII----TLVSYKIKITVSLFGFVLNLWAFSFFHRFEKQSHFCFMLKHLSLVELIFSLSGVVFECFYFTLVVLQRPFPKVLVNFIIYFVFNFMWLMYKSSLLSRNWVIVLIAWRRYQAIHRPXXXXXXXXXXXXXXXIFGIYAISLFLVTPRLFEITYSYSNETKNVIITPYLLVNS-----------IYLSTYPILFFILISPVPVCVICFYSIFMLRSLRKPECHELETRSDHFQRQNPTXXXXXXXXXXXXNKXXXXXXXXXXXCEFPMCLAGIFNQISRESTGVQFQWFLT-HFAGVANILTSCDSIFNFVIYFISFPFFKQSILKGFSAISC 1086 ETD I TL+ + I + F FV+ F K+S +L+ LSL E++F+++ V+ E + Q P LV FI +V ++ L++RNW +V++A RY+A+ RP+ S + + I I+A+S+ + PR+ ETK I P L VN +YL T IL F++ + PV ++CF S +L+ +RK + + R +F + + S ++ +++L F E P F++I Q + L H +AN L DSI N +Y IS P F+ S S + C Sbjct: 29 ETDTIYKYITLILTPLSILSNTFAFVV------FKESIWKKSVMSKILRMLSLFEILFNVN-VLLEASIRHKIEEQHPSMVELVIFI--YVITAAKVLMIVFLIARNWTVVILAGYRYEAVCRPVGSCKYIRYNNINLAFCFIFALSILIALPRMM--------ETKIEICQPTLSVNRFVPFLISYKWYLYLHT-GILLFLIQNGGPVILVCFLSSCVLKVIRKMK----KIRKSNFNLADKK-----GSPQPSGDRLIVILSAAFFVLETP----AFFSKILHHYFEYQGYYNLDIHIGLLANTLIYLDSIINVAVYVISNPTFRISSKALLSKLIC 336
BLAST of SMED30032138 vs. Planmine SMEST
Match: SMESG000023730.1 (SMESG000023730.1) HSP 1 Score: 60.077 bits (144), Expect = 1.322e-9 Identity = 85/344 (24.71%), Postives = 150/344 (43.60%), Query Frame = -2 Query: 43 ITVSLFGFVLNLWAFSFFHRFEKQSHFCFMLKHLSLVELIFSLSGVVFECFYFTLVVLQRPFPKVLVNFIIYFVFNFMWLMYKSSLLSRNWVIVLIAWRRYQAIHRPXXXXXXXXXXXXXXXIFGIYAISLFLVTPRLFEITYSYSNETKNVIITPYLLVNSIYLSTY-PILFFILISPVPVCVICFYSIFMLRSLRKPECHELETRSDHFQRQNPTXXXXXXXXXXXXNKXXXXXXXXXXXCEFPMCLA-GIFNQISRESTGVQFQWFLTH-------FAGVANILTSCDSIFNFVIYFISFPFFKQSILKGFSAISCYDFQNNYRLDNSNFSRQKTINQCEE 1047 I++ GF L F FF + K+S +LK+LS E++F++SG ++ F + P F+ S L+ RNW I+ +A R+ +I RPL++K++ ++ L++ Y ++ + PR FE ++ N+++ P LL Y Y L FI+ + PV SI ++R++ K + ++ S + +N IS +CLA +F S S +Q + A VAN+L DS+ N +Y +S P F++ LK ++ + Y + + R++TI+Q +E Sbjct: 278 ISIIFNGFCL----FIFFDKIWKKSILSILLKYLSATEIVFNISGFLYSVTKF----IDYPSINQFSKLFWVFILQIGTFSVASGLICRNWTILHLAIYRFDSICRPLNTKKIYTETSTYKLLWITYILAAVIAFPRTFEYKATFCE--GNLVLKPSLLNYDWYAYGYQGSLIFIIQTAAPVVCTSILSISVMRAILKVKRFR-KSFSKTIKTKNNAISGDALMIF--------------------ICLAFFLFETPSFFSKALQSYLMRNNKIIEDVLLATVANLLVYLDSVINIFVYILSNPVFRKK-LKQIFHFKAFNLK-EYPTEATALERRETISQSKE 588
BLAST of SMED30032138 vs. Planmine SMEST
Match: SMESG000004763.1 (SMESG000004763.1) HSP 1 Score: 57.7658 bits (138), Expect = 5.039e-9 Identity = 75/286 (26.22%), Postives = 142/286 (49.65%), Query Frame = -2 Query: 121 MLKHLSLVELIFSLSGVVFECFYFTLVVLQRPFPKVLVN-FIIYFVFNFMWLMYKSSLLSRNWVIVLIAWRRYQAIHRPXXXXXXXXXXXXXXXIFGIYAISLFLVTPRLFEITYSYSNETKNVIITPYLLVNS-----IYLSTYPILFFILISPVPVCVICFYSIFMLRSLRKPECHELETRSDHFQRQNPTXXXXXXXXXXXXNKXXXXXXXXXXXCEFPMCLAGIFNQISRESTGVQFQWFLTHFAGVANILTSCDSIFNFVIYFISFPFFKQSILKGFSAIS 960 +L S +ELIF ++ ++F + + + + VN I+ +F ++L L++RN I+L+A+ R++ I PL +K+ ++ I + FGI + ++ + PR+FE TY+Y +T +V+ L N IYL+ + +F I I +P+ + SI MLR +R+ + + + ++++ +++K V+ LC F T E P F+++ S + + H +ANI DS N +IY +S F+ ++L+ S +S Sbjct: 84 ILARFSFIELIFQMTLLLFYLVEY-IKIDEYIISSRFVNPGIMDALFRCIYLFSVGFLIARNSWIILLAFHRFECICYPLRTKRYVTSANINFYFFGIVSFAILIAFPRMFEQTYTYCLQTGDVVNVSEALSNVYQYRIIYLNVF--VFLIQIG-IPLLSVNILSILMLRKIRQSLTFRRTSAAPNQKKKS-----------FAADKLVVALCITFFTFEMP----SFFSKLFHNSHIISNDKIIYHLTFIANISIYIDSSMNLLIYLVSNRIFRGAVLQQISKVS 350 The following BLAST results are available for this feature:
BLAST of SMED30032138 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30032138 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30032138 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30032138 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30032138 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30032138 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30032138 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30032138 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 3
BLAST of SMED30032138 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30032138 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30032138 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30032138 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30032138 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30032138 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30032138 ID=SMED30032138|Name=SMED30032138|organism=Schmidtea mediterranea sexual|type=transcript|length=1159bpback to top protein sequence of SMED30032138-orf-1 >SMED30032138-orf-1 ID=SMED30032138-orf-1|Name=SMED30032138-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=370bp MFNFTINESSKGINYETDIITLVSYKIKITVSLFGFVLNLWAFSFFHRFEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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