SMED30032106
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30032106 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 12
Alignments
SMED30032106 aligns in the following genomic locations:
Homology
BLAST of SMED30032106 vs. Ensembl Celegans
Match: C30G7.2 (pep chromosome:WBcel235:V:14936898:14939367:1 gene:WBGene00044176.1 transcript:C30G7.2b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:C30G7.2) HSP 1 Score: 82.4185 bits (202), Expect = 3.003e-19 Identity = 53/149 (35.57%), Postives = 71/149 (47.65%), Query Frame = 1 Query: 193 QLSKTDALACYLCDSQNGSNTACEDPLNPAY---------TDKVNLKNPCMQGRPGSIGEFWAQYCIKIKATKSIDKS-TLTIRRCTLDKLTET-------LNHCGLFKLNDIDYEGCISSCTKDACNSSRVPQ-RLDYYFCMLFILFS 585 + S + C++C S +G N CEDP N + N PC + G G F A +CIKI ++ ++S TL IR C LD T T ++HCG FK Y+GC+ SC D CNSS V L F ++F L S Sbjct: 38 EASGVSGIGCFVCSSFDGENKGCEDPFNSTMDLSSRDRDASAVANYNYPCWAYKKGRHGLFPADHCIKIVGYRADNESKTLVIRTCALDSGTLTADTEIVRISHCGSFKYEGHQYKGCVQSCDTDGCNSSSVHSFLLPLCFLIVFSLLS 186
BLAST of SMED30032106 vs. Ensembl Celegans
Match: C30G7.2 (pep chromosome:WBcel235:V:14936898:14938942:1 gene:WBGene00044176.1 transcript:C30G7.2a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:C30G7.2) HSP 1 Score: 80.8777 bits (198), Expect = 8.700e-19 Identity = 52/148 (35.14%), Postives = 70/148 (47.30%), Query Frame = 1 Query: 196 LSKTDALACYLCDSQNGSNTACEDPLNPAY---------TDKVNLKNPCMQGRPGSIGEFWAQYCIKIKATKSIDKS-TLTIRRCTLDKLTET-------LNHCGLFKLNDIDYEGCISSCTKDACNSSRVPQ-RLDYYFCMLFILFS 585 + + C++C S +G N CEDP N + N PC + G G F A +CIKI ++ ++S TL IR C LD T T ++HCG FK Y+GC+ SC D CNSS V L F ++F L S Sbjct: 37 MEGVSGIGCFVCSSFDGENKGCEDPFNSTMDLSSRDRDASAVANYNYPCWAYKKGRHGLFPADHCIKIVGYRADNESKTLVIRTCALDSGTLTADTEIVRISHCGSFKYEGHQYKGCVQSCDTDGCNSSSVHSFLLPLCFLIVFSLLS 184
BLAST of SMED30032106 vs. Ensembl Fly
Match: CG31323 (gene:FBgn0051323 transcript:FBtr0307339) HSP 1 Score: 84.3445 bits (207), Expect = 4.710e-20 Identity = 48/113 (42.48%), Postives = 64/113 (56.64%), Query Frame = 1 Query: 220 CYLCDSQNGSNTACEDPLNPAYTDKVNLKNPCMQGRPGSIGEFWAQYCIKIKATKSIDKSTLTIRRCTLDKLTET-------LNHCGLFKLNDIDYEGCISSCT-KDACNSSR 534 C+ C S NG+N AC+DP + Y+ + L++PCM GR G G F A CIKI T+T+R C LD T T ++HCG F +D GC+ SC+ DACNS+R Sbjct: 30 CFKCVSYNGANKACDDPFHNNYSTAI-LESPCMGGRKGRDGLFPATACIKIAGYYDGTGETITVRGCALDSGTLTTDTEIIRMSHCGKFYYDDKYVHGCLQSCSDADACNSAR 141
BLAST of SMED30032106 vs. Ensembl Fly
Match: CG31323 (gene:FBgn0051323 transcript:FBtr0084988) HSP 1 Score: 84.3445 bits (207), Expect = 4.710e-20 Identity = 48/113 (42.48%), Postives = 64/113 (56.64%), Query Frame = 1 Query: 220 CYLCDSQNGSNTACEDPLNPAYTDKVNLKNPCMQGRPGSIGEFWAQYCIKIKATKSIDKSTLTIRRCTLDKLTET-------LNHCGLFKLNDIDYEGCISSCT-KDACNSSR 534 C+ C S NG+N AC+DP + Y+ + L++PCM GR G G F A CIKI T+T+R C LD T T ++HCG F +D GC+ SC+ DACNS+R Sbjct: 30 CFKCVSYNGANKACDDPFHNNYSTAI-LESPCMGGRKGRDGLFPATACIKIAGYYDGTGETITVRGCALDSGTLTTDTEIIRMSHCGKFYYDDKYVHGCLQSCSDADACNSAR 141
BLAST of SMED30032106 vs. TrEMBL
Match: A0A183L9A0 (Uncharacterized protein OS=Schistosoma margrebowiei OX=48269 PE=4 SV=1) HSP 1 Score: 115.546 bits (288), Expect = 4.603e-29 Identity = 58/130 (44.62%), Postives = 82/130 (63.08%), Query Frame = 1 Query: 205 TDALACYLCDSQNGSNTACEDPLNPAYTDKVNLKNPCMQGRPGSIGEFWAQYCIKIKATKSIDKSTLTIRRCTLDKLTETLNHCGLFKLNDIDYEGCISSCTKDACNSSRVPQ---RLDYYFCMLFILFS 585 T A+ACY C S N SN CEDP++ + ++ PC Q PG G F+A YC KIK + D S+L IRRC++DKLT+ HCG F+L+D Y GCI++C+KD CN+ +P R+ FC+L + ++ Sbjct: 5 TTAIACYSCVSMNKSNPPCEDPVSA----HITIQRPCRQTIPGHEGLFYAHYCTKIKGIRQKDGSSLLIRRCSMDKLTDIPTHCGQFQLDDDLYHGCIATCSKDWCNTGDIPSVSVRM-LTFCLLILSYT 129
BLAST of SMED30032106 vs. TrEMBL
Match: A0A3P7Z3J1 (Uncharacterized protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS375 PE=4 SV=1) HSP 1 Score: 115.546 bits (288), Expect = 4.755e-29 Identity = 58/130 (44.62%), Postives = 82/130 (63.08%), Query Frame = 1 Query: 205 TDALACYLCDSQNGSNTACEDPLNPAYTDKVNLKNPCMQGRPGSIGEFWAQYCIKIKATKSIDKSTLTIRRCTLDKLTETLNHCGLFKLNDIDYEGCISSCTKDACNSSRVPQ---RLDYYFCMLFILFS 585 T A+ACY C S N SN CEDP++ + ++ PC Q PG G F+A YC KIK + D S+L IRRC++DKLT+ HCG F+L+D Y GCI++C+KD CN+ +P R+ FC+L + ++ Sbjct: 5 TTAIACYSCVSMNKSNPPCEDPVSA----HITIQRPCRQTIPGHEGLFYAHYCTKIKGIRQKDGSSLLIRRCSMDKLTDIPTHCGQFQLDDDLYHGCIATCSKDWCNTGDIPSVSVRM-LTFCLLILSYT 129
BLAST of SMED30032106 vs. TrEMBL
Match: A0A4Z2CVG4 (Splicing factor 4 OS=Schistosoma japonicum OX=6182 GN=EWB00_007257 PE=4 SV=1) HSP 1 Score: 111.694 bits (278), Expect = 3.378e-27 Identity = 54/112 (48.21%), Postives = 75/112 (66.96%), Query Frame = 1 Query: 193 QLSKTDALACYLCDSQNGSNTACEDPLNPAYTDKVNLKNPCMQGRPGSIGEFWAQYCIKIKATKSIDKSTLTIRRCTLDKLTETLNHCGLFKLNDIDYEGCISSCTKDACNS 528 +++ T A+ACY C S N SN ACEDP+ + +V ++ PC Q PG G F+A YC KIK + D S+L IRRC++DKL + HCG F+L+D Y GCI++C+KD CN+ Sbjct: 28 EVNITTAIACYSCVSMNRSNPACEDPI----SAEVTIQRPCRQTIPGHKGLFYAHYCSKIKGIRQKDGSSLLIRRCSMDKLADIPTHCGQFQLDDDLYHGCIATCSKDWCNN 135
BLAST of SMED30032106 vs. TrEMBL
Match: A0A3S5BZ36 (Uncharacterized protein OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS18846 PE=4 SV=1) HSP 1 Score: 111.309 bits (277), Expect = 4.232e-27 Identity = 51/112 (45.54%), Postives = 74/112 (66.07%), Query Frame = 1 Query: 196 LSKTDALACYLCDSQNGSNTACEDPLNPAYTDKVNLKNPCMQGRPGSIGEFWAQYCIKIKATKSIDKSTLTIRRCTLDKLTETLNHCGLFKLNDIDYEGCISSCTKDACNSS 531 L DA++CY C+S NGSN CEDP++ V ++ PCMQG G F+A++C+KIK + D L +RRC+++KL + + HCG FKLN+ Y GCI++C KD CN++ Sbjct: 27 LLLADAISCYSCNSVNGSNQPCEDPMD----GSVGVEKPCMQGISDHKGLFFARFCLKIKGRRQSDGQLLVMRRCSMEKLADIMTHCGEFKLNEDLYSGCIATCNKDWCNAA 134
BLAST of SMED30032106 vs. TrEMBL
Match: A0A3P7M0B3 (Uncharacterized protein OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS11619 PE=4 SV=1) HSP 1 Score: 108.612 bits (270), Expect = 2.428e-26 Identity = 50/108 (46.30%), Postives = 70/108 (64.81%), Query Frame = 1 Query: 202 KTDALACYLCDSQNGSNTACEDPLNPAYTDKVNLKNPCMQGRPGSIGEFWAQYCIKIKATKSIDKSTLTIRRCTLDKLTETLNHCGLFKLNDIDYEGCISSCTKDACN 525 + LACY+C S NGS CEDP+ + + PC QGRPG G +A+YC+KIK + D T+ IRRC++DKL + HCG F+L+D Y+GCI++CT++ CN Sbjct: 5 EAQGLACYVCTSVNGSYPDCEDPVK----GSIPFEAPCRQGRPGYEGLSYARYCVKIKGERVSDGQTIYIRRCSMDKLADVPTHCGQFRLSDDLYQGCIATCTQNWCN 108
BLAST of SMED30032106 vs. Planmine SMEST
Match: SMESG000080318.1 (SMESG000080318.1) HSP 1 Score: 283.108 bits (723), Expect = 2.487e-98 Identity = 136/137 (99.27%), Postives = 136/137 (99.27%), Query Frame = 1 Query: 187 DSQLSKTDALACYLCDSQNGSNTACEDPLNPAYTDKVNLKNPCMQGRPGSIGEFWAQYCIKIKATKSIDKSTLTIRRCTLDKLTETLNHCGLFKLNDIDYEGCISSCTKDACNSSRVPQRLDYYFCMLFILFSSQVV 597 DSQLSKTDALACYLCDSQNGSNTACEDPLNPAYTDKVNLKNPCMQGRPGSIGEFWAQYCIKIKATKSIDKSTLTIRRCTLDKLTETLNHCGLFKLNDIDYEGCISSCTKDACNSSRVPQRLDYYFCML ILFSSQVV Sbjct: 14 DSQLSKTDALACYLCDSQNGSNTACEDPLNPAYTDKVNLKNPCMQGRPGSIGEFWAQYCIKIKATKSIDKSTLTIRRCTLDKLTETLNHCGLFKLNDIDYEGCISSCTKDACNSSRVPQRLDYYFCMLLILFSSQVV 150 The following BLAST results are available for this feature:
BLAST of SMED30032106 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30032106 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 2
BLAST of SMED30032106 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 2
BLAST of SMED30032106 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30032106 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30032106 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30032106 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30032106 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30032106 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30032106 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30032106 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30032106 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30032106 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30032106 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30032106 ID=SMED30032106|Name=SMED30032106|organism=Schmidtea mediterranea sexual|type=transcript|length=691bpback to top protein sequence of SMED30032106-orf-1 >SMED30032106-orf-1 ID=SMED30032106-orf-1|Name=SMED30032106-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=151bp MAIELILLFIFAIDSQLSKTDALACYLCDSQNGSNTACEDPLNPAYTDKVback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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