SMED30032065

Overview
NameSMED30032065
Smed IDSMED30032065
Length (bp)1363
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30032065 (SMED30032065) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30032065 (SMED30032065) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30032065

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30032065SMESG000075340.1 SmedASXL_005872SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30032065SMESG000075340.1 dd_Smed_v4_76503_0_2dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30032065SMESG000075340.1 dd_Smed_v4_76503_0_2dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30032065SMESG000075340.1 dd_Smed_v4_76503_0_2dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
reproductive organSMED30032065SMESG000075340.1 Contig14616newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30032065SMESG000075340.1 Contig14616uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30032065 vs. Ensembl Celegans
Match: amt-3 (Putative ammonium transporter 3 [Source:UniProtKB/Swiss-Prot;Acc:Q21565])

HSP 1 Score: 87.4261 bits (215), Expect = 3.964e-18
Identity = 52/150 (34.67%), Postives = 83/150 (55.33%), Query Frame = -3 
Query:  423 LQYLVNL*FVLI*ITDILAGCTVIETWEAIIIGFIASFIVYFGAKLILWLRIDDPIGATAVHGFGGMWGLLSVGLFCDLDPYQNQSRNN-IGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLI-KFLVGIRLTPHDELIGADISEH 866
            + +L+N   +L  I  I A C V   W A++IG I+S        L+  L IDDP+G   +H    +WG+++VG+FC+ D Y   + NN  GL +   F+ L +Q      ++++     F+ L LI K  +G+R+T ++E IGAD+ EH
Sbjct:  299 VNFLIN--GILSSIVSITAICAVSRPWHALVIGSISSVFSIAVLPLLDRLHIDDPVGIVPIHLTSSIWGMIAVGIFCEEDKYLGSATNNRSGLLYSWSFELLWVQLQCTAAILIYSATTGFLALFLISKSPLGLRVTDYEEQIGADVIEH 446
[Close]
BLAST of SMED30032065 vs. Ensembl Celegans
Match: amt-2 (Putative ammonium transporter 2 [Source:UniProtKB/Swiss-Prot;Acc:Q20605])

HSP 1 Score: 84.7297 bits (208), Expect = 2.234e-17
Identity = 61/204 (29.90%), Postives = 98/204 (48.04%), Query Frame = -3 
Query:  243 GCTVIETWEAIIIGFIASFIVYFGAKLILWLRIDDPIGATAVHGFGGMWGLLSVGLFCDLDP---------YQNQSRNNIGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLIKF-LVGIRLTPHDELIGADISEHDVGXXXXXXXXXXXIYNVQFR--RRRLSGILMKESNFKKQLDKIWEKIYKKTND---VRPQSVDS 809
            GC     W+A ++G I S +      +  WL+IDDP+G   VH  G +WG+++  +F    P         +Q     N GL +GGGF  L LQ+   + +  +  I  FIIL LI    VG+R+  + E +GAD+ EH +          +  Y ++ +   + LS +LM    ++ +  K+  +  KK +D   V PQ  +S
Sbjct:  310 GCLYFTPWQATLVGAIGSSLALAAYPVTEWLKIDDPVGVFPVHVVGSIWGMIAPAIFVYRRPMNFGPPECDFQTSDEIN-GLLYGGGFYLLFLQSFVILVIGTYSAICAFIILFLIHHSPVGLRVDKYTEELGADLIEHGLA------GFNVMTYTIEKKLDTKTLSAVLMIIVRWRAK-AKLGAQRRKKIHDSGSVAPQQAES 505
[Close]
BLAST of SMED30032065 vs. Ensembl Celegans
Match: amt-4 (Ammonium transporter [Source:UniProtKB/TrEMBL;Acc:Q17663])

HSP 1 Score: 70.4774 bits (171), Expect = 9.263e-13
Identity = 47/136 (34.56%), Postives = 68/136 (50.00%), Query Frame = -3 
Query:  423 AGCTVIETWEAIIIGFIASFIVYFGAKLILWLRIDDPIGATAVHGFGGMWGLLSVGLFCDLDPYQNQSRNNIGLFHGGG------FKSLGLQTLTCICLIVWGCIATFIILGLIKFLVGIRLTPHDELIGADISEH 812
            AGC  +E W  I +G  A  I    +KL++ L+IDDP+ A AVH  GG WGL SV  F   D       N IG    GG      F  LG Q +  + ++ W  +  + I  L++ +  +R++   E+ G DI +H
Sbjct:  309 AGCNKMEPWACIWVGVGAGLIYLGLSKLMVRLKIDDPLDAFAVHAGGGFWGLTSVA-FIGHDGVVYSIGNTIGGATNGGDQIAQAFAQLGWQWVCALAIVTWSILWMWPIFALLRKIGKLRVSAEVEINGLDIYKH 443
[Close]
BLAST of SMED30032065 vs. Ensembl Celegans
Match: amt-1 (Putative ammonium transporter 1 [Source:UniProtKB/Swiss-Prot;Acc:P54145])

HSP 1 Score: 62.7734 bits (151), Expect = 2.750e-10
Identity = 43/136 (31.62%), Postives = 67/136 (49.26%), Query Frame = -3 
Query:  423 AGCTVIETWEAIIIGFIASFIVYFGAKLILWLRIDDPIGATAVHGFGGMWGLLSV------GLFCDLDPYQNQSRNNIGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLIKFLVGIRLTPHDELIGADISEH 812
            AGC  +E W  I +G  A  I    +KL++ L+IDDP+ A AVH  GG WGL+S       G+   L    + ++N+ G      F  LG Q +  + +I W       I  ++K    +R++   E+ G D+ +H
Sbjct:  309 AGCNKMEPWACIWVGLGAGLIYLAFSKLMIRLKIDDPLDAFAVHAGGGFWGLMSSSIISHGGVAYALADAVSGAKNS-GDHLTQAFAQLGWQMICALAIIAWSLGVMLPIFWILKKTGKLRVSEEVEINGLDVFKH 443
[Close]
BLAST of SMED30032065 vs. Ensembl Fly
Match: Amt (gene:FBgn0038309 transcript:FBtr0113238)

HSP 1 Score: 127.487 bits (319), Expect = 1.472e-31
Identity = 59/137 (43.07%), Postives = 89/137 (64.96%), Query Frame = -3 
Query:  417 ITDILAGCTVIETWEAIIIGFIASFIVYFGAKLILWLRIDDPIGATAVHGFGGMWGLLSVGLFCDLDPYQNQSRNNIGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLIKFLVGIRLTPHDELIGADISEHDV 827
            +  I AGC +   WEA++IG I S        L   + +DDP+GA+AVHG  G+WG+++VGLF D     + +    GLF GGG+  LG+QTL+ +CL  WG  +TF++L +I  ++ IR+ PH+EL+GAD++EH +
Sbjct:  294 LVSITAGCFLYRAWEALVIGAIGSLFCVLAMPLFDRMGVDDPVGASAVHGVCGIWGVIAVGLFADNPIPLDTTNGRSGLFKGGGWYLLGIQTLSALCLACWGVCSTFLLLYVINKIIPIRMDPHEELLGADLTEHRI 430
[Close]
BLAST of SMED30032065 vs. UniProt/SwissProt
Match: sp|Q9BLG3|AMT2_DICDI (Ammonium transporter 2 OS=Dictyostelium discoideum OX=44689 GN=amtB PE=1 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 6.387e-19
Identity = 53/130 (40.77%), Postives = 78/130 (60.00%), Query Frame = -3 
Query:  432 AGCTVIETWEAIIIGFIASFIVYFGAKLILWLRIDDPIGATAVHGFGGMWGLLSVGLFC---DLDPYQNQSRNNIGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLIKFLVGIRLTPHDELIGADI 812
            A C++I+ W A IIG + SF+    + L++ LRIDDP+ ++A+H   G+WG LSVGLF    +L     +S N  GLF GGGF+ LG+Q L  I ++ W      I+  ++K    +R+ P  EL+G DI
Sbjct:  290 AVCSLIDPWAAFIIGCVTSFVYLGCSHLLIKLRIDDPLDSSAIHLGCGIWGALSVGLFSTQENLSLVLKKSTNVYGLFFGGGFEQLGIQLLGIIIVMAWCFFCASILFTILKKFHLLRIEPTKELMGIDI 419
[Close]
BLAST of SMED30032065 vs. UniProt/SwissProt
Match: sp|P54147|Y108_SYNY3 (Putative ammonium transporter sll0108 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=sll0108 PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 2.938e-17
Identity = 50/141 (35.46%), Postives = 80/141 (56.74%), Query Frame = -3 
Query:  417 VLI*ITDILAGCTVIETWEAIIIGFIASFIVYFGAKLILWLRIDDPIGATAVHGFGGMWGLLSVGLFCDLDPYQNQSRNNIGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLIKFLVGIRLTPHDELIGADISEHDV 839
            VL  +  I AGC  +  W ++IIG IA  +V +       ++IDDP+GA +VH   G+WG L+VG F       N  +   GLF+GGG   L +Q +  + +  +  I +F++  ++K  +GIR++  +E+IG DI EH +
Sbjct:  353 VLAGLVGITAGCAGVSYWGSVIIGGIAGILVVYSVAFFDKIKIDDPVGAISVHLVNGVWGTLAVGFF-------NMEK---GLFYGGGINQLIIQIVGILAIGAFTAIFSFVVWAILKQTMGIRVSGEEEMIGLDIGEHGM 483
[Close]
BLAST of SMED30032065 vs. UniProt/SwissProt
Match: sp|Q21565|AMT3_CAEEL (Putative ammonium transporter 3 OS=Caenorhabditis elegans OX=6239 GN=amt-3 PE=3 SV=2)

HSP 1 Score: 87.4261 bits (215), Expect = 5.042e-17
Identity = 52/150 (34.67%), Postives = 83/150 (55.33%), Query Frame = -3 
Query:  423 LQYLVNL*FVLI*ITDILAGCTVIETWEAIIIGFIASFIVYFGAKLILWLRIDDPIGATAVHGFGGMWGLLSVGLFCDLDPYQNQSRNN-IGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLI-KFLVGIRLTPHDELIGADISEH 866
            + +L+N   +L  I  I A C V   W A++IG I+S        L+  L IDDP+G   +H    +WG+++VG+FC+ D Y   + NN  GL +   F+ L +Q      ++++     F+ L LI K  +G+R+T ++E IGAD+ EH
Sbjct:  299 VNFLIN--GILSSIVSITAICAVSRPWHALVIGSISSVFSIAVLPLLDRLHIDDPVGIVPIHLTSSIWGMIAVGIFCEEDKYLGSATNNRSGLLYSWSFELLWVQLQCTAAILIYSATTGFLALFLISKSPLGLRVTDYEEQIGADVIEH 446
[Close]
BLAST of SMED30032065 vs. UniProt/SwissProt
Match: sp|Q20605|AMT2_CAEEL (Putative ammonium transporter 2 OS=Caenorhabditis elegans OX=6239 GN=amt-2 PE=3 SV=3)

HSP 1 Score: 84.7297 bits (208), Expect = 2.842e-16
Identity = 61/204 (29.90%), Postives = 98/204 (48.04%), Query Frame = -3 
Query:  243 GCTVIETWEAIIIGFIASFIVYFGAKLILWLRIDDPIGATAVHGFGGMWGLLSVGLFCDLDP---------YQNQSRNNIGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLIKF-LVGIRLTPHDELIGADISEHDVGXXXXXXXXXXXIYNVQFR--RRRLSGILMKESNFKKQLDKIWEKIYKKTND---VRPQSVDS 809
            GC     W+A ++G I S +      +  WL+IDDP+G   VH  G +WG+++  +F    P         +Q     N GL +GGGF  L LQ+   + +  +  I  FIIL LI    VG+R+  + E +GAD+ EH +          +  Y ++ +   + LS +LM    ++ +  K+  +  KK +D   V PQ  +S
Sbjct:  310 GCLYFTPWQATLVGAIGSSLALAAYPVTEWLKIDDPVGVFPVHVVGSIWGMIAPAIFVYRRPMNFGPPECDFQTSDEIN-GLLYGGGFYLLFLQSFVILVIGTYSAICAFIILFLIHHSPVGLRVDKYTEELGADLIEHGLA------GFNVMTYTIEKKLDTKTLSAVLMIIVRWRAK-AKLGAQRRKKIHDSGSVAPQQAES 505
[Close]
BLAST of SMED30032065 vs. UniProt/SwissProt
Match: sp|P54144|AMT11_ARATH (Ammonium transporter 1 member 1 OS=Arabidopsis thaliana OX=3702 GN=AMT1-1 PE=1 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 7.814e-16
Identity = 50/137 (36.50%), Postives = 73/137 (53.28%), Query Frame = -3 
Query:  423 ILAGCTVIETWEAIIIGFIASFIVYFGAKLILWLRIDDPIGATAVHGFGGMWGLLSVGLFCDLDPYQNQSRNNI-----GLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLIKFLVGIRLTPHDELIGADISEH 818
            I  GC+V+E W AII GF+A+ ++    KL   L+ DDP+ A  +HG  G WGL+   LF   + Y NQ   N      GLF GGG K LG Q +  I +  W       +  ++K +  +R++  DE+ G D++ H
Sbjct:  327 ITGGCSVVEPWAAIICGFVAALVLLGCNKLAEKLKYDDPLEAAQLHGGCGAWGLIFTALFAQ-EKYLNQIYGNKPGRPHGLFMGGGGKLLGAQLIQIIVITGWVSATMGTLFFILKKMKLLRISSEDEMAGMDMTRH 462
[Close]
BLAST of SMED30032065 vs. TrEMBL
Match: A0A210QRI3 (Ammonium transporter OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT14651 PE=3 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 3.820e-39
Identity = 73/151 (48.34%), Postives = 106/151 (70.20%), Query Frame = -3 
Query:  414 LQYLVNL*FVLI*ITDILAGCTVIETWEAIIIGFIASFIVYFGAKLILWLRIDDPIGATAVHGFGGMWGLLSVGLFCDLDPYQNQSRNNIGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLIKFLVGIRLTPHDELIGADISEHDVG 866
            + YLVN   VL  +  I AGC VI  WEA++IG I   I     KL+  +RIDDP+GATAVHG GG+WG+L+VGLF + +P +N ++   G+FHGGGF  +G+Q L+ +CL+ W  + +F++L +IK+ VG+RL+  +E +GADI EH++G
Sbjct:  266 VDYLVNC--VLCALVSITAGCAVIRPWEALLIGAIGGVICIGSTKLMDKMRIDDPVGATAVHGAGGIWGMLAVGLFAENEPLENTTQGRAGVFHGGGFYLMGIQMLSIVCLVAWSAVTSFLLLMVIKYTVGLRLSEEEERLGADIVEHNIG 414
[Close]
BLAST of SMED30032065 vs. TrEMBL
Match: A0A1I8IRJ7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 8.364e-38
Identity = 75/167 (44.91%), Postives = 107/167 (64.07%), Query Frame = -3 
Query:  330 ITDILAGCTVIETWEAIIIGFIASFIVYFGAKLILWLRIDDPIGATAVHGFGGMWGLLSVGLFCDLDPYQNQSRNNIGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLIKFLVGIRLTPHDELIGADISEHDVGXXXXXXXXXXXIY-NVQFRRRRLSGILMK 827
            +  I AGC+V+  WEA++IGFI   IV     ++  L+IDDP+GA  VHG GG+WG+L+ G+F D DP    +    GL HGGGF  LG+Q LTC+C+  W  I T + LGL+K  +GIR+T  +EL+GAD+ EH++G      L   + Y + + R RR SG+++K
Sbjct:  213 MVSISAGCSVVRPWEAVVIGFIGGLIVVPVVPIMDKLKIDDPVGAFPVHGLGGLWGMLACGIFVDQDPLDGMNNGQKGLLHGGGFHLLGVQLLTCVCIAFWSAIVTVLSLGLLKLTMGIRMTIEEELLGADLFEHNIGTVDQDCLMEEKRYFSSRVRPRRYSGLVVK 379
[Close]
BLAST of SMED30032065 vs. TrEMBL
Match: A0A1I8GC89 (Ammonium transporter OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 1.650e-36
Identity = 81/196 (41.33%), Postives = 118/196 (60.20%), Query Frame = -3 
Query:  252 VLI*ITDILAGCTVIETWEAIIIGFIASFIVYFGAKLILWLRIDDPIGATAVHGFGGMWGLLSVGLFCDLDPYQNQSRNNIGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLIKFLVGIRLTPHDELIGADISEHDVGXXXXXXXXXXXIYNVQFRRRRLSGILMKESNFKKQLDKIWEKIYKKTNDVRPQS 839
            +L  +  I AGC+++  WEA++IG I   I    A L+  + +DDP+GA AVHGF G+WG+L+ G+F D+DP QN +    GLFHGGGF  LG+Q L C+CL  W    TF+ LG +K  +GIRL+P +EL+G+D  EH++  E  +  E+   +  Q R+RR S +L K       L  I E+    + DV  ++
Sbjct:  346 ILASLVSITAGCSIVRAWEAVVIGIIGGIIAIPSAILMDKIHLDDPVGAVAVHGFAGVWGMLACGIFVDVDPLQNMNNGQKGLFHGGGFYLLGVQLLACVCLGAWSAGVTFVTLGTLKLTIGIRLSPEEELLGSDFVEHNIPNEEHVASEVPPEFLNQARQRRFSALLAKNWTSDSDLKIIEEENAGASPDVNGEA 541
[Close]
BLAST of SMED30032065 vs. TrEMBL
Match: A0A1I8GAW9 (Ammonium transporter OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 2.485e-36
Identity = 81/196 (41.33%), Postives = 118/196 (60.20%), Query Frame = -3 
Query:  252 VLI*ITDILAGCTVIETWEAIIIGFIASFIVYFGAKLILWLRIDDPIGATAVHGFGGMWGLLSVGLFCDLDPYQNQSRNNIGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLIKFLVGIRLTPHDELIGADISEHDVGXXXXXXXXXXXIYNVQFRRRRLSGILMKESNFKKQLDKIWEKIYKKTNDVRPQS 839
            +L  +  I AGC+++  WEA++IG I   I    A L+  + +DDP+GA AVHGF G+WG+L+ G+F D+DP QN +    GLFHGGGF  LG+Q L C+CL  W    TF+ LG +K  +GIRL+P +EL+G+D  EH++  E  +  E+   +  Q R+RR S +L K       L  I E+    + DV  ++
Sbjct:  483 ILASLVSITAGCSIVRAWEAVVIGIIGGIIAIPSAILMDKIHLDDPVGAVAVHGFAGVWGMLACGIFVDVDPLQNMNNGQKGLFHGGGFYLLGVQLLACVCLGAWSAGVTFVTLGTLKLTIGIRLSPEEELLGSDFVEHNIPNEEHVASEVPPEFLNQARQRRFSALLAKNWTSDSDLKIIEEENAGASPDVNGEA 678
[Close]
BLAST of SMED30032065 vs. TrEMBL
Match: A0A210PR83 (Ammonium transporter OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT15122 PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 1.715e-31
Identity = 66/150 (44.00%), Postives = 93/150 (62.00%), Query Frame = -3 
Query:  417 LQYLVNL*FVLI*ITDILAGCTVIETWEAIIIGFIASFIVYFGAKLILWLRIDDPIGATAVHGFGGMWGLLSVGLFCDLDPYQNQSRNNIGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLIKFLVGIRLTPHDELIGADISEHDV 866
            ++Y+VN   +L  +  I A C +I  WEA+I G +  FI     K   WL +DDP+GA +VHG  G+WGL+ VGLF + D  +N +    GL HGGGF  LG+Q +  + L  W  + TFI+L  IK  +GIRL+PH+E +GAD  EH++
Sbjct:  282 IEYIVNS--ILGGLVSITAACAIIRPWEALIAGAVGGFIAIHAPKFFNWLHVDDPVGAVSVHGVVGIWGLMVVGLFTENDHLENLTLGRSGLIHGGGFYLLGVQMMAALILSTWSSLCTFILLFPIKMTIGIRLSPHEEFLGADYVEHNI 429
[Close]
BLAST of SMED30032065 vs. Ensembl Nematostella
Match: EDO41793 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3L2])

HSP 1 Score: 115.161 bits (287), Expect = 5.268e-28
Identity = 55/137 (40.15%), Postives = 85/137 (62.04%), Query Frame = -3 
Query:  417 ITDILAGCTVIETWEAIIIGFIASFIVYFGAKLILWLRIDDPIGATAVHGFGGMWGLLSVGLFCDLDPYQNQSRNNIGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLIKFLVGIRLTPHDELIGADISEHDV 827
            +  I A C+V   WEA++IG I + I   G  L+  L+IDDP+G    H F G+WGL++VGLF + D  + +  N+ G+F GG ++ LG+Q L  + ++ WG + TF+ L LI  +  +R+T  +EL+GAD  EH +
Sbjct:  304 LVSITAICSVARPWEALVIGSIGALIACSGCSLLEKLKIDDPVGCVPTHAFAGIWGLIAVGLFAEKDTLEGRFSNSYGVFKGGPWRFLGVQLLLIVAVVSWGAVTTFLELKLIDKIFPLRVTLENELLGADDYEHGI 440
[Close]
BLAST of SMED30032065 vs. Ensembl Nematostella
Match: EDO40423 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S731])

HSP 1 Score: 113.235 bits (282), Expect = 3.703e-27
Identity = 59/137 (43.07%), Postives = 83/137 (60.58%), Query Frame = -3 
Query:  417 ITDILAGCTVIETWEAIIIGFIASFIVYFGAKLILWLRIDDPIGATAVHGFGGMWGLLSVGLFCDLDPYQNQSRNNIGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLIKFLVGIRLTPHDELIGADISEHDV 827
            +  I A C V+  WE+++IGFI   I   G  L+  LRIDDP+G  A HG  G+WGLL+VGLF ++D  +N S    G+F GG +  LG+Q L  + +  W  +  FI+L +I  +VG+R+    EL GADI EH +
Sbjct:  290 LVGITAICAVVRQWESLLIGFIGGMIACVGDALVKKLRIDDPVGCIATHGMAGIWGLLAVGLFVEVDNLENFSA-EFGVFKGGSWHLLGIQLLVVVSIGAWAAVTNFILLYVIDKVVGLRMPLAMELDGADIWEHGI 425
[Close]
BLAST of SMED30032065 vs. Ensembl Nematostella
Match: EDO47038 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNC3])

HSP 1 Score: 101.293 bits (251), Expect = 2.312e-23
Identity = 54/130 (41.54%), Postives = 78/130 (60.00%), Query Frame = -3 
Query:  417 CTVIETWEAIIIGFIASFIVYFGAKLILWLRIDDPIGATAVHGFGGMWGLLSVGLFCDLDPYQNQSRNNIGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLIKFLVGIRLTPHDELIGADISEHDV 806
            C +   WE+I+IG I   +  FG  LI  L+IDDP G  A H   G+WGL+SVG F ++D  ++ S  N G+  GG    LG+Q LT + LI W    +FI+L ++  LVG+R+T  +E +G D  EH++
Sbjct:  273 CPLARPWESILIGTIGGVLSCFGRNLIQKLKIDDPTGCLAAHILSGIWGLVSVGFFTEVDNLEHFSTIN-GVLKGGNGWLLGIQLLTVVALIAWSAAVSFILLMILNKLVGLRVTEEEENLGCDAVEHEM 401
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BLAST of SMED30032065 vs. Ensembl Nematostella
Match: EDO34221 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQ16])

HSP 1 Score: 94.7449 bits (234), Expect = 3.380e-21
Identity = 52/137 (37.96%), Postives = 76/137 (55.47%), Query Frame = -3 
Query:  417 ITDILAGCTVIETWEAIIIGFIASFIVYFGAKLILWLRIDDPIGATAVHGFGGMWGLLSVGLFCDLDPYQNQSRNNIGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLIKFLVGIRLTPHDELIGADISEHDV 827
            +  I A C ++  WEAI+IG I   I   G  L+  LRIDDP+G    H F  +WGL+ VGL  + D   + S+   G+  GG ++ LG Q L  +C+ VW  + TF++L  +  +V +R+    EL GAD  EH +
Sbjct:  290 LVSITAICAIVRPWEAILIGAIGGAIACGGCSLLNRLRIDDPVGCVPTHFFASVWGLIVVGLLVEKDSLMDLSK-EYGVLKGGSWRLLGAQLLAIVCISVWSAVMTFVMLCGVDRVVKLRMPLIMELEGADKWEHGI 425
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BLAST of SMED30032065 vs. Ensembl Nematostella
Match: EDO37226 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SGD4])

HSP 1 Score: 90.8929 bits (224), Expect = 6.265e-20
Identity = 45/131 (34.35%), Postives = 77/131 (58.78%), Query Frame = -3 
Query:  414 CTVIETWEAIIIGFIASFIVYFGAKLILWLRIDDPIGATAVHGFGGMWGLLSVGLFCDLDPYQNQSRNNIGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLIKFLVGIRLTPHDELIGADISEHDVG 806
            C     WEAI++G + S I  +G  L+  +RIDDP+G+ ++H F  +W +++ G+F  L+P  +  R   GL   G   +LG++ L  + + VW  +   +IL  I   +G+R++  +E++GADI EH +G
Sbjct:  277 CGWARPWEAILVGAVGSLIACYGVSLLERVRIDDPVGSVSIHMFPAIWAMVAAGIF--LEPDASAKRPG-GLVQTGSAYTLGVELLATVVVSVWCALMATVILFFIDKTIGLRVSYQEEVLGADIVEHSIG 404
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BLAST of SMED30032065 vs. Planmine SMEST
Match: SMESG000075340.1 (SMESG000075340.1)

HSP 1 Score: 328.176 bits (840), Expect = 9.297e-112
Identity = 190/190 (100.00%), Postives = 190/190 (100.00%), Query Frame = -3 
Query:   93 GLLSVGLFCDLDPYQNQSRNNIGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLIKFLVGIRLTPHDELIGADISEHDVGXXXXXXXXXXXIYNVQFRRRRLSGILMKESNFKKQLDKIWEKIYKKTNDVRPQSVDSKMDQHRNSDTRRSSMGSYXXXXXXXXXXXXXXXXSWVNTEPMKSNLSVVM 662
            GLLSVGLFCDLDPYQNQSRNNIGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLIKFLVGIRLTPHDELIGADISEHDVGEEIPLNLELLEIYNVQFRRRRLSGILMKESNFKKQLDKIWEKIYKKTNDVRPQSVDSKMDQHRNSDTRRSSMGSYKVVKLNKSSVLSKKSNSWVNTEPMKSNLSVVM
Sbjct:   17 GLLSVGLFCDLDPYQNQSRNNIGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLIKFLVGIRLTPHDELIGADISEHDVGEEIPLNLELLEIYNVQFRRRRLSGILMKESNFKKQLDKIWEKIYKKTNDVRPQSVDSKMDQHRNSDTRRSSMGSYKVVKLNKSSVLSKKSNSWVNTEPMKSNLSVVM 206
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BLAST of SMED30032065 vs. Planmine SMEST
Match: SMESG000075340.1 (SMESG000075340.1)

HSP 1 Score: 327.791 bits (839), Expect = 1.175e-111
Identity = 190/190 (100.00%), Postives = 190/190 (100.00%), Query Frame = -3 
Query:   93 GLLSVGLFCDLDPYQNQSRNNIGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLIKFLVGIRLTPHDELIGADISEHDVGXXXXXXXXXXXIYNVQFRRRRLSGILMKESNFKKQLDKIWEKIYKKTNDVRPQSVDSKMDQHRNSDTRRSSMGSYXXXXXXXXXXXXXXXXSWVNTEPMKSNLSVVM 662
            GLLSVGLFCDLDPYQNQSRNNIGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLIKFLVGIRLTPHDELIGADISEHDVGEEIPLNLELLEIYNVQFRRRRLSGILMKESNFKKQLDKIWEKIYKKTNDVRPQSVDSKMDQHRNSDTRRSSMGSYKVVKLNKSSVLSKKSNSWVNTEPMKSNLSVVM
Sbjct:   10 GLLSVGLFCDLDPYQNQSRNNIGLFHGGGFKSLGLQTLTCICLIVWGCIATFIILGLIKFLVGIRLTPHDELIGADISEHDVGEEIPLNLELLEIYNVQFRRRRLSGILMKESNFKKQLDKIWEKIYKKTNDVRPQSVDSKMDQHRNSDTRRSSMGSYKVVKLNKSSVLSKKSNSWVNTEPMKSNLSVVM 199
The following BLAST results are available for this feature:
BLAST of SMED30032065 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032065 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
amt-33.964e-1834.67Putative ammonium transporter 3 [Source:UniProtKB... [more]
amt-22.234e-1729.90Putative ammonium transporter 2 [Source:UniProtKB... [more]
amt-49.263e-1334.56Ammonium transporter [Source:UniProtKB/TrEMBL;Acc... [more]
amt-12.750e-1031.62Putative ammonium transporter 1 [Source:UniProtKB... [more]
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BLAST of SMED30032065 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Amt1.472e-3143.07gene:FBgn0038309 transcript:FBtr0113238[more]
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BLAST of SMED30032065 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032065 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032065 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032065 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9BLG3|AMT2_DICDI6.387e-1940.77Ammonium transporter 2 OS=Dictyostelium discoideum... [more]
sp|P54147|Y108_SYNY32.938e-1735.46Putative ammonium transporter sll0108 OS=Synechocy... [more]
sp|Q21565|AMT3_CAEEL5.042e-1734.67Putative ammonium transporter 3 OS=Caenorhabditis ... [more]
sp|Q20605|AMT2_CAEEL2.842e-1629.90Putative ammonium transporter 2 OS=Caenorhabditis ... [more]
sp|P54144|AMT11_ARATH7.814e-1636.50Ammonium transporter 1 member 1 OS=Arabidopsis tha... [more]
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BLAST of SMED30032065 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A210QRI33.820e-3948.34Ammonium transporter OS=Mizuhopecten yessoensis OX... [more]
A0A1I8IRJ78.364e-3844.91Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8GC891.650e-3641.33Ammonium transporter OS=Macrostomum lignano OX=282... [more]
A0A1I8GAW92.485e-3641.33Ammonium transporter OS=Macrostomum lignano OX=282... [more]
A0A210PR831.715e-3144.00Ammonium transporter OS=Mizuhopecten yessoensis OX... [more]
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BLAST of SMED30032065 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032065 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032065 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032065 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO417935.268e-2840.15Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO404233.703e-2743.07Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO470382.312e-2341.54Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO342213.380e-2137.96Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO372266.265e-2034.35Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30032065 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30032065 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000075340.19.297e-112100.00SMESG000075340.1[more]
SMESG000075340.11.175e-111100.00SMESG000075340.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30032065 ID=SMED30032065|Name=SMED30032065|organism=Schmidtea mediterranea sexual|type=transcript|length=1363bp
TTTTTTTTAAAATGAAATTTAGACGTTATATTATTATCATATGAAAGAAT
TCATAGAATTATCTTTTTAATTTATCCAAGATGTCAAATGTGCATTACAA
CAGATAAATTTGATTTCATTGGTTCCGTATTTACCCACGAATTCGATTTT
TTTGAAAGGACCGAGGACTTGTTAAGCTTTACAACTTTATAGGATCCCAT
TGAAGATCGTCTGGTATCAGAATTTCTATGTTGGTCCATTTTCGAATCCA
CAGATTGAGGTCTTACGTCATTGGTTTTTTTGTAAATTTTCTCCCAAATC
TTATCCAATTGTTTTTTGAAATTCGATTCTTTCATCAGAATTCCCGATAA
ACGTCGCCTTCTAAATTGTACATTGTATATTTCAAGGAGTTCTAGATTGA
GTGGAATTTCTTCACCCACATCATGCTCAGATATATCAGCTCCAATTAAT
TCATCATGCGGTGTCAAACGAATTCCGACAAGAAACTTAATCAATCCTAA
AATGATAAAGGTTGCTATACATCCCCAAACAATTAGACAGATGCATGTCA
AAGTTTGCAGTCCTAGACTCTTGAATCCGCCTCCATGAAAAAGACCAATA
TTATTTCTGGATTGGTTTTGGTATGGATCTAGATCACAGAACAATCCCAC
TGATAGCAATCCCCACATTCCTCCAAACCCATGAACAGCTGTAGCTCCGA
TTGGATCATCAATCCTTAACCATAGGATCAGTTTGGCACCAAAATACACA
ATGAAGGATGCTATAAAACCAATAATAATAGCCTCCCATGTTTCAATTAC
TGTACACCCAGCTAGAATATCAGTAATTCAGATTAAAACGAATCAAAGGT
TTACCAGATATTGCAACCAACCCACTTAGCACAGAACTAACGAAAGTTGA
AACTTTATATTTTCCTCCAGTTTCAATTATGCTGTTATTAATTTCAATGG
TTTTGAAAATGAAATTACAGATGAAAAGCTCACGTAATCAGTATACCACT
TATTCCAGCTCCGATCGAAGAATTCAATGTGTTCACAACTGCTCTGGAAA
AATTTTAACAATAACAAAGAATCAATTCATACGAGATGCACTCAAGAAAG
AAGGATATTTTCTCCTGAGAAATGAGAGCGATGTGATGAAGCTCATTTAA
GACAATTTGCAATATAAAAAACCCAGCTGATATCTGAAACCCAGTCGAAA
ATTGTAAGAGGACCAAATTTCAATATCACAGTTGATGAATATACAACTGT
CCTTCGTCGAAGATTTTTTGAAATCAATCTCAATTGCATGAAGAGAATAA
AACCTATAAAACAGGAATTGTAAGAATATTTGGATCGTTTAGTGCAGAAT
CGATGACCGAAAT
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protein sequence of SMED30032065-orf-1

>SMED30032065-orf-1 ID=SMED30032065-orf-1|Name=SMED30032065-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=195bp
MWGLLSVGLFCDLDPYQNQSRNNIGLFHGGGFKSLGLQTLTCICLIVWGC
IATFIILGLIKFLVGIRLTPHDELIGADISEHDVGEEIPLNLELLEIYNV
QFRRRRLSGILMKESNFKKQLDKIWEKIYKKTNDVRPQSVDSKMDQHRNS
DTRRSSMGSYKVVKLNKSSVLSKKSNSWVNTEPMKSNLSVVMHI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR029020Ammonium/urea_transptr
IPR024041NH4_transpt_AmtB-like_dom
Vocabulary: molecular function
TermDefinition
GO:0008519ammonium transmembrane transporter activity
Vocabulary: biological process
TermDefinition
GO:0015696ammonium transport
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029020Ammonium/urea transporterGENE3DG3DSA:1.10.3430.10coord: 1..87
e-value: 4.9E-14
score: 54.1
IPR024041Ammonium transporter AmtB-like domainPFAMPF00909Ammonium_transpcoord: 1..83
e-value: 2.6E-11
score: 42.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 136..154
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 136..157
NoneNo IPR availablePANTHERPTHR11730AMMONIUM TRANSPORTERcoord: 1..174
NoneNo IPR availablePANTHERPTHR11730:SF58AMMONIUM TRANSPORTERcoord: 1..174
NoneNo IPR availableSUPERFAMILYSSF111352Ammonium transportercoord: 1..68
NoneNo IPR availableTMHMMTMhelixcoord: 37..59