DNA-binding protein

Overview


Name	DNA-binding protein
Smed ID	SMED30032043
Length (bp)	1294

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of DNA-binding protein (SMED30032043) t-SNE clustered cells

Violin plots show distribution of expression levels for DNA-binding protein (SMED30032043) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DNA-binding protein (SMED30032043) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DNA-binding protein (SMED30032043) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30032043

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
nervous system	SMED30032043	h1SMcG0022016	dd_Smed_v4_22163_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30032043	h1SMcG0022016	dd_Smed_v4_22163_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
non-ciliated neuron	SMED30032043	h1SMcG0022016	dd_Smed_v4_22163_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cilated neuron	SMED30032043	h1SMcG0022016	dd_Smed_v4_22163_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Homology

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BLAST of DNA-binding protein vs. Ensembl Human
Match: UNCX (UNC homeobox [Source:HGNC Symbol;Acc:HGNC:33194])

HSP 1 Score: 153.68 bits (387), Expect = 1.030e-40
Identity = 70/111 (63.06%), Postives = 80/111 (72.07%), Query Frame = 2

Query:  518 CKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISP 850
            C +  +   F++ D  D +      KRRRTRTNF  WQL++LE  F  SHYPDV  RE+LA  L L ESRVQVWFQNRRAKWRKKENTKKGPGRPAHN+ P TCSGEP+ P
Sbjct:   79 CGVGGDGQPFKLSDSGDPDKESPGCKRRRTRTNFTGWQLEELEKAFNESHYPDVFMREALALRLDLVESRVQVWFQNRRAKWRKKENTKKGPGRPAHNSHPTTCSGEPMDP 189

[Close]

BLAST of DNA-binding protein vs. Ensembl Human
Match: RAX2 (retina and anterior neural fold homeobox 2 [Source:HGNC Symbol;Acc:HGNC:18286])

HSP 1 Score: 93.5893 bits (231), Expect = 9.684e-22
Identity = 43/70 (61.43%), Postives = 51/70 (72.86%), Query Frame = 2

Query:  587 EEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPG 796
            ++K RR RT F  +QL QLE  F+ SHYPDV SRE LA  + LPE RVQVWFQNRRAKWR++E  + G G
Sbjct:   24 KKKHRRNRTTFTTYQLHQLERAFEASHYPDVYSREELAAKVHLPEVRVQVWFQNRRAKWRRQERLESGSG 93

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Query:  587 EEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPG 796
            ++K RR RT F  +QL QLE  F+ SHYPDV SRE LA  + LPE RVQVWFQNRRAKWR++E  + G G
Sbjct:   24 KKKHRRNRTTFTTYQLHQLERAFEASHYPDVYSREELAAKVHLPEVRVQVWFQNRRAKWRRQERLESGSG 93

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BLAST of DNA-binding protein vs. Ensembl Human
Match: SHOX (short stature homeobox [Source:HGNC Symbol;Acc:HGNC:10853])

HSP 1 Score: 94.3597 bits (233), Expect = 1.075e-21
Identity = 48/99 (48.48%), Postives = 66/99 (66.67%), Query Frame = 2

Query:  482 FSSTNNSSQLDNCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKEN 778
            F +   +  +  CK K ED +      S++E   T+ K+RR+RTNF + QL++LE +F  +HYPD   RE L++ LGL E+RVQVWFQNRRAK RK+EN
Sbjct:   85 FGTARVAEGIYECKEKREDVK------SEDEDGQTKLKQRRSRTNFTLEQLNELERLFDETHYPDAFMREELSQRLGLSEARVQVWFQNRRAKCRKQEN 177

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Query:  482 FSSTNNSSQLDNCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKEN 778
            F +   +  +  CK K ED +      S++E   T+ K+RR+RTNF + QL++LE +F  +HYPD   RE L++ LGL E+RVQVWFQNRRAK RK+EN
Sbjct:   85 FGTARVAEGIYECKEKREDVK------SEDEDGQTKLKQRRSRTNFTLEQLNELERLFDETHYPDAFMREELSQRLGLSEARVQVWFQNRRAKCRKQEN 177

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BLAST of DNA-binding protein vs. Ensembl Celegans
Match: unc-4 (Homeobox protein unc-4 [Source:UniProtKB/Swiss-Prot;Acc:P29506])

HSP 1 Score: 108.612 bits (270), Expect = 6.717e-27
Identity = 54/93 (58.06%), Postives = 66/93 (70.97%), Query Frame = 2

Query:  512 DNCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKG 790
            DN  +  +D    I  E DN+T ++  KRRRTRTNF+ WQL++LE  F+ SHYPDV  RE+LA  L L ESRVQVWFQNRRAKWRK+E  + G
Sbjct:   63 DNLLMNEDD---GIALEDDNDTGESAAKRRRTRTNFSGWQLEELESAFEASHYPDVFMREALAMRLDLLESRVQVWFQNRRAKWRKREQNRNG 152

[Close]

BLAST of DNA-binding protein vs. Ensembl Celegans
Match: alr-1 (AristaLess (Drosophila homeodomain) Related [Source:UniProtKB/TrEMBL;Acc:Q21836])

HSP 1 Score: 88.5817 bits (218), Expect = 4.672e-19
Identity = 56/134 (41.79%), Postives = 75/134 (55.97%), Query Frame = 2

Query:  422 SQIKLNGLANFPISFAPKISFSS-TNNSSQL---DNCKIKSEDFQFQIKDESDNETVDT------------EEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKE 775
            +Q+  N    FP SF P  S ++ TNN  +L   D  K  + D   +     DN    +            + K+RR RT F+ +QLD+LE VF  +HYPDV +RE LA  + L E+RVQVWFQNRRAK+RK+E
Sbjct:   44 AQLFANPALQFP-SFPPAFSIAALTNNQHELKEDDGKKTPTGDNILEAASVLDNRENGSPSDGTNSPDDNGKRKQRRYRTTFSAFQLDELEKVFARTHYPDVFTREELATRVQLTEARVQVWFQNRRAKYRKQE 176

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BLAST of DNA-binding protein vs. Ensembl Celegans
Match: ceh-8 (Homeobox protein ceh-8 [Source:UniProtKB/Swiss-Prot;Acc:Q94398])

HSP 1 Score: 85.1149 bits (209), Expect = 2.268e-18
Identity = 37/63 (58.73%), Postives = 48/63 (76.19%), Query Frame = 2

Query:  587 EEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKE 775
            ++K+RR RT F  +QL  LE  F  +HYPDV +RE+LA  + LPE RVQVWFQNRRAK+R++E
Sbjct:   57 DKKQRRNRTTFTTFQLHALEAAFDKTHYPDVYARETLAAKVQLPEVRVQVWFQNRRAKFRRQE 119

[Close]

BLAST of DNA-binding protein vs. Ensembl Celegans
Match: ceh-10 (Homeobox protein ceh-10 [Source:UniProtKB/Swiss-Prot;Acc:P41935])

HSP 1 Score: 85.8853 bits (211), Expect = 2.749e-18
Identity = 38/66 (57.58%), Postives = 48/66 (72.73%), Query Frame = 2

Query:  584 TEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENT 781
            ++ K+RR RT F  +Q+D+LE  F+ SHYPD+ +RE LA    L E R+QVWFQNRRAKWRK E T
Sbjct:  132 SKRKKRRHRTIFTQYQIDELEKAFQDSHYPDIYAREVLAGKTELQEDRIQVWFQNRRAKWRKTEKT 197

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BLAST of DNA-binding protein vs. Ensembl Celegans
Match: ceh-8 (Homeobox protein ceh-8 [Source:UniProtKB/Swiss-Prot;Acc:Q94398])

HSP 1 Score: 84.3445 bits (207), Expect = 4.259e-18
Identity = 37/63 (58.73%), Postives = 48/63 (76.19%), Query Frame = 2

Query:  587 EEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKE 775
            ++K+RR RT F  +QL  LE  F  +HYPDV +RE+LA  + LPE RVQVWFQNRRAK+R++E
Sbjct:   55 DKKQRRNRTTFTTFQLHALEAAFDKTHYPDVYARETLAAKVQLPEVRVQVWFQNRRAKFRRQE 117

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BLAST of DNA-binding protein vs. Ensembl Fly
Match: unc-4 (gene:FBgn0024184 transcript:FBtr0340626)

HSP 1 Score: 136.732 bits (343), Expect = 8.267e-35
Identity = 63/86 (73.26%), Postives = 70/86 (81.40%), Query Frame = 2

Query:  593 KRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISP 850
            KRRR+RTNF  WQL++LE  F  SHYPD+  RE+LA  L L ESRV VWFQNRRAK RK+E+TKKGPGRPAHNAQP TCSGEPI P
Sbjct:  242 KRRRSRTNFNSWQLEELERAFSASHYPDIFMREALAMRLDLKESRVAVWFQNRRAKVRKREHTKKGPGRPAHNAQPQTCSGEPIPP 327

[Close]

BLAST of DNA-binding protein vs. Ensembl Fly
Match: unc-4 (gene:FBgn0024184 transcript:FBtr0340627)

HSP 1 Score: 136.732 bits (343), Expect = 8.267e-35
Identity = 63/86 (73.26%), Postives = 70/86 (81.40%), Query Frame = 2

Query:  593 KRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISP 850
            KRRR+RTNF  WQL++LE  F  SHYPD+  RE+LA  L L ESRV VWFQNRRAK RK+E+TKKGPGRPAHNAQP TCSGEPI P
Sbjct:  242 KRRRSRTNFNSWQLEELERAFSASHYPDIFMREALAMRLDLKESRVAVWFQNRRAKVRKREHTKKGPGRPAHNAQPQTCSGEPIPP 327

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BLAST of DNA-binding protein vs. Ensembl Fly
Match: OdsH (gene:FBgn0026058 transcript:FBtr0074461)

HSP 1 Score: 132.88 bits (333), Expect = 1.576e-34
Identity = 58/84 (69.05%), Postives = 68/84 (80.95%), Query Frame = 2

Query:  593 KRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPI 844
            K+RR RTNF  WQL +LE VF+ SHYPD+  RE+LA  L L E R+ VWFQNRRAKWRK+E+TKKGPGRPAHNA P +CSG+PI
Sbjct:  157 KKRRGRTNFNSWQLRELERVFQGSHYPDIFMREALATKLDLMEGRIAVWFQNRRAKWRKQEHTKKGPGRPAHNAHPQSCSGDPI 240

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BLAST of DNA-binding protein vs. Ensembl Fly
Match: Rx (gene:FBgn0020617 transcript:FBtr0342783)

HSP 1 Score: 97.4413 bits (241), Expect = 2.672e-21
Identity = 56/129 (43.41%), Postives = 74/129 (57.36%), Query Frame = 2

Query:  407 KTNSDSQIKLNGLANFPISFAPKISFSSTNNSSQLDNCKIKSEDFQFQIKDESDNETVD---TEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTK 784
            + N+DS   +NG      S A     + TN+S Q +           +I   SD+E  D    ++K RR RT F  +QL +LE  F+ SHYPDV SRE LA  + LPE RVQVWFQNRRAKWR++E ++
Sbjct:  510 RLNADSDSLVNG------SCASSEDLNQTNSSEQGE-----------KITSGSDDEGQDDNCAKKKHRRNRTTFTTYQLHELERAFEKSHYPDVYSREELAMKVNLPEVRVQVWFQNRRAKWRRQEKSE 621

[Close]

BLAST of DNA-binding protein vs. Ensembl Fly
Match: al (gene:FBgn0000061 transcript:FBtr0078053)

HSP 1 Score: 90.5077 bits (223), Expect = 1.767e-19
Identity = 41/67 (61.19%), Postives = 51/67 (76.12%), Query Frame = 2

Query:  593 KRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGP 793
            K+RR RT F  +QL++LE  F  +HYPDV +RE LA  +GL E+R+QVWFQNRRAKWRK+E  K GP
Sbjct:   84 KQRRYRTTFTSFQLEELEKAFSRTHYPDVFTREELAMKIGLTEARIQVWFQNRRAKWRKQE--KVGP 148

[Close]

BLAST of DNA-binding protein vs. Ensembl Zebrafish
Match: uncx (UNC homeobox [Source:ZFIN;Acc:ZDB-GENE-080509-1])

HSP 1 Score: 153.295 bits (386), Expect = 3.854e-41
Identity = 70/112 (62.50%), Postives = 82/112 (73.21%), Query Frame = 2

Query:  515 NCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISP 850
             C +  ++ Q+++ D  D +      KRRRTRTNF  WQL++LE  F  SHYPDV  RE+LA  L L ESRVQVWFQNRRAKWRKKENTKKGPGRPAHN+ P TCSGEP+ P
Sbjct:   73 GCGMNGDNQQYKLTDSGDPDKDSPGCKRRRTRTNFTGWQLEELEKAFNESHYPDVFMREALALRLDLIESRVQVWFQNRRAKWRKKENTKKGPGRPAHNSHPTTCSGEPMDP 184

[Close]

BLAST of DNA-binding protein vs. Ensembl Zebrafish
Match: uncx4.1 (Unc4.1 homeobox (C. elegans) [Source:NCBI gene;Acc:553947])

HSP 1 Score: 152.14 bits (383), Expect = 7.794e-41
Identity = 71/112 (63.39%), Postives = 81/112 (72.32%), Query Frame = 2

Query:  515 NCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISP 850
             C +   D Q+++ D  D +      KRRRTRTNF  WQL++LE  F  SHYPDV  RE+LA  L L ESRVQVWFQNRRAKWRKKENTKKGPGRPAHN+ P TCSGEP+ P
Sbjct:   75 GCGVNG-DSQYKLGDGGDPDKESPGCKRRRTRTNFTGWQLEELEKAFNESHYPDVFMREALALRLDLVESRVQVWFQNRRAKWRKKENTKKGPGRPAHNSHPTTCSGEPMDP 185

[Close]

BLAST of DNA-binding protein vs. Ensembl Zebrafish
Match: shox (short stature homeobox [Source:ZFIN;Acc:ZDB-GENE-051030-21])

HSP 1 Score: 98.9821 bits (245), Expect = 5.460e-23
Identity = 50/99 (50.51%), Postives = 68/99 (68.69%), Query Frame = 2

Query:  482 FSSTNNSSQLDNCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKEN 778
            FS T  S  + +CK K ED +      S++E   ++ K+RR+RTNF + QL++LE +F  +HYPD   RE L++ LGL E+RVQVWFQNRRAK RK+EN
Sbjct:   77 FSVTRASDGIYDCKDKKEDVK------SEDEDAQSKLKQRRSRTNFTLEQLNELERLFDETHYPDAFMREELSQRLGLSEARVQVWFQNRRAKCRKQEN 169

[Close]

BLAST of DNA-binding protein vs. Ensembl Zebrafish
Match: shox2 (short stature homeobox 2 [Source:ZFIN;Acc:ZDB-GENE-040426-1457])

HSP 1 Score: 90.8929 bits (224), Expect = 8.106e-21
Identity = 47/106 (44.34%), Postives = 69/106 (65.09%), Query Frame = 2

Query:  461 SFAPKISFSSTNNSSQLDNCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKEN 778
            S +PK++  +T+   + ++CK   ++ Q +IK             +RR+RTNF + QL++LE +F  +HYPD   RE L++ LGL E+RVQVWFQNRRAK RK+EN
Sbjct:   10 SSSPKLTDGNTDMKERKEDCKPLEDETQTKIK-------------QRRSRTNFTLEQLNELERLFDETHYPDAFMREELSQRLGLSEARVQVWFQNRRAKCRKQEN 102

[Close]

BLAST of DNA-binding protein vs. Ensembl Zebrafish
Match: rx2 (retinal homeobox gene 2 [Source:NCBI gene;Acc:30473])

HSP 1 Score: 92.8189 bits (229), Expect = 1.406e-20
Identity = 46/97 (47.42%), Postives = 59/97 (60.82%), Query Frame = 2

Query:  512 DNCKIKSEDFQFQIKDESDNETVDTEE----KRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKG 790
            D C     D +  ++ +S +  +  E+    K RR RT F  +QL +LE  F+ SHYPDV SRE LA  + LPE RVQVWFQNRRAKWR++E    G
Sbjct:  103 DKCDADLGDPRSNVESDSRSPDIPDEDQPKKKHRRNRTTFTTYQLHELERAFEKSHYPDVYSREELAMKVNLPEVRVQVWFQNRRAKWRRQEKMDTG 199

[Close]

BLAST of DNA-binding protein vs. Ensembl Xenopus
Match: uncx (UNC homeobox [Source:NCBI gene;Acc:100492016])

HSP 1 Score: 153.295 bits (386), Expect = 6.393e-41
Identity = 70/112 (62.50%), Postives = 81/112 (72.32%), Query Frame = 2

Query:  515 NCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISP 850
             C +  +  Q+++ D  D +      KRRRTRTNF  WQL++LE  F  SHYPDV  RE+LA  L L ESRVQVWFQNRRAKWRKKENTKKGPGRPAHN+ P TCSGEP+ P
Sbjct:   74 GCGLNGDSQQYKLSDSIDPDKESPGCKRRRTRTNFTGWQLEELEKAFNESHYPDVFMREALALRLDLVESRVQVWFQNRRAKWRKKENTKKGPGRPAHNSHPTTCSGEPMDP 185

[Close]

BLAST of DNA-binding protein vs. Ensembl Xenopus
Match: SHOX (short stature homeobox [Source:NCBI gene;Acc:100486480])

HSP 1 Score: 96.2857 bits (238), Expect = 1.791e-21
Identity = 49/99 (49.49%), Postives = 67/99 (67.68%), Query Frame = 2

Query:  482 FSSTNNSSQLDNCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKEN 778
            + ST  S  +  CK K +D +      S++E   T+ K+RR+RTNF + QL++LE +F  +HYPD   RE L++ LGL E+RVQVWFQNRRAK RK+EN
Sbjct:  124 YGSTRVSEGIYECKEKRDDVK------SEDEDGQTKLKQRRSRTNFTLEQLNELERLFDETHYPDAFMREELSQRLGLSEARVQVWFQNRRAKCRKQEN 216

[Close]

BLAST of DNA-binding protein vs. Ensembl Xenopus
Match: ENSXETT00000028778.1 (retina and anterior neural fold homeobox [Source:NCBI gene;Acc:100038124])

HSP 1 Score: 93.5893 bits (231), Expect = 1.069e-20
Identity = 45/75 (60.00%), Postives = 53/75 (70.67%), Query Frame = 2

Query:  563 SDNETVDTEE----KRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKE 775
            SDN+  D E+    K RR RT F  +QL +LE  F+ SHYPDV SRE LA  + LPE RVQVWFQNRRAKWR++E
Sbjct:  116 SDNKLSDDEQQPKKKHRRNRTTFTTYQLHELERAFEKSHYPDVYSREELAMKVNLPEVRVQVWFQNRRAKWRRQE 190

[Close]

BLAST of DNA-binding protein vs. Ensembl Xenopus
Match: gja10 (gap junction protein alpha 10 [Source:Xenbase;Acc:XB-GENE-962101])

HSP 1 Score: 90.1225 bits (222), Expect = 1.403e-20
Identity = 38/89 (42.70%), Postives = 59/89 (66.29%), Query Frame = 2

Query:  509 LDNCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKE 775
            ++  + +  D + ++ D +   +    + +RR RT F + QL +LE +F+ +HYPDV +R+ LA  + LPE+RVQ+WFQNRRAKWRK E
Sbjct:    5 INGFEAEHHDGEDELSDSAQESSTCDRKNKRRIRTTFTLAQLQELEQIFQVTHYPDVQTRDQLAAKIQLPEARVQIWFQNRRAKWRKYE 93

[Close]

BLAST of DNA-binding protein vs. Ensembl Xenopus
Match: ENSXETT00000045475.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:8:38234894:38239775:1 gene:ENSXETG00000019174.1 transcript:ENSXETT00000045475.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 90.8929 bits (224), Expect = 2.239e-20
Identity = 42/68 (61.76%), Postives = 52/68 (76.47%), Query Frame = 2

Query:  593 KRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPG 796
            K+RR RT F+  QL++LE+ F+ SHYPDV +RE LA  L L E+RVQVWFQNRRAKWRK+E T+   G
Sbjct:   51 KQRRYRTTFSNLQLEELEMAFRKSHYPDVFTREDLAMRLDLTEARVQVWFQNRRAKWRKREKTEMLGG 118

[Close]

BLAST of DNA-binding protein vs. Ensembl Mouse
Match: Uncx (UNC homeobox [Source:MGI Symbol;Acc:MGI:108013])

HSP 1 Score: 155.221 bits (391), Expect = 1.575e-41
Identity = 71/111 (63.96%), Postives = 80/111 (72.07%), Query Frame = 2

Query:  518 CKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISP 850
            C +  E   F++ D  D +      KRRRTRTNF  WQL++LE  F  SHYPDV  RE+LA  L L ESRVQVWFQNRRAKWRKKENTKKGPGRPAHN+ P TCSGEP+ P
Sbjct:   83 CGVAGESQPFKLADSGDPDKESPGCKRRRTRTNFTGWQLEELEKAFNESHYPDVFMREALALRLDLVESRVQVWFQNRRAKWRKKENTKKGPGRPAHNSHPTTCSGEPMDP 193

[Close]

BLAST of DNA-binding protein vs. Ensembl Mouse
Match: Alx3 (aristaless-like homeobox 3 [Source:MGI Symbol;Acc:MGI:1277097])

HSP 1 Score: 89.3521 bits (220), Expect = 4.853e-21
Identity = 42/72 (58.33%), Postives = 53/72 (73.61%), Query Frame = 2

Query:  572 ETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKK 787
            E   T+ K+RR RT F+ +QL++LE VF+ +HYPDV +RE LA    L E+RVQVWFQNRRAKWRK+E   K
Sbjct:    2 ELAKTKSKKRRNRTTFSTFQLEELEKVFQKTHYPDVYAREQLALRTDLTEARVQVWFQNRRAKWRKRERYGK 73

[Close]

BLAST of DNA-binding protein vs. Ensembl Mouse
Match: Alx3 (aristaless-like homeobox 3 [Source:MGI Symbol;Acc:MGI:1277097])

HSP 1 Score: 92.8189 bits (229), Expect = 2.255e-20
Identity = 42/72 (58.33%), Postives = 53/72 (73.61%), Query Frame = 2

Query:  572 ETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKK 787
            E   T+ K+RR RT F+ +QL++LE VF+ +HYPDV +RE LA    L E+RVQVWFQNRRAKWRK+E   K
Sbjct:  145 ELAKTKSKKRRNRTTFSTFQLEELEKVFQKTHYPDVYAREQLALRTDLTEARVQVWFQNRRAKWRKRERYGK 216

[Close]

BLAST of DNA-binding protein vs. Ensembl Mouse
Match: Shox2 (short stature homeobox 2 [Source:MGI Symbol;Acc:MGI:1201673])

HSP 1 Score: 85.8853 bits (211), Expect = 5.308e-20
Identity = 40/65 (61.54%), Postives = 52/65 (80.00%), Query Frame = 2

Query:  584 TEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKEN 778
            T+ K+RR+RTNF + QL++LE +F  +HYPD   RE L++ LGL E+RVQVWFQNRRAK RK+EN
Sbjct:    7 TKIKQRRSRTNFTLEQLNELERLFDETHYPDAFMREELSQRLGLSEARVQVWFQNRRAKCRKQEN 71

[Close]

BLAST of DNA-binding protein vs. Ensembl Mouse
Match: Rax (retina and anterior neural fold homeobox [Source:MGI Symbol;Acc:MGI:109632])

HSP 1 Score: 91.6633 bits (226), Expect = 5.670e-20
Identity = 42/74 (56.76%), Postives = 51/74 (68.92%), Query Frame = 2

Query:  557 DESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKEN 778
            D   +E    ++K RR RT F  +QL +LE  F+ SHYPDV SRE LA  + LPE RVQVWFQNRRAKWR++E 
Sbjct:  123 DSKLSEEEPPKKKHRRNRTTFTTYQLHELERAFEKSHYPDVYSREELAGKVNLPEVRVQVWFQNRRAKWRRQEK 196

[Close]

BLAST of DNA-binding protein vs. UniProt/SwissProt
Match: sp|O08934|UNC4_MOUSE (Homeobox protein unc-4 homolog OS=Mus musculus OX=10090 GN=Uncx PE=2 SV=2)

HSP 1 Score: 155.221 bits (391), Expect = 1.102e-40
Identity = 71/111 (63.96%), Postives = 80/111 (72.07%), Query Frame = 2

Query:  518 CKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISP 850
            C +  E   F++ D  D +      KRRRTRTNF  WQL++LE  F  SHYPDV  RE+LA  L L ESRVQVWFQNRRAKWRKKENTKKGPGRPAHN+ P TCSGEP+ P
Sbjct:   83 CGVAGESQPFKLADSGDPDKESPGCKRRRTRTNFTGWQLEELEKAFNESHYPDVFMREALALRLDLVESRVQVWFQNRRAKWRKKENTKKGPGRPAHNSHPTTCSGEPMDP 193

[Close]

BLAST of DNA-binding protein vs. UniProt/SwissProt
Match: sp|P97830|UNC4_RAT (Homeobox protein unc-4 homolog OS=Rattus norvegicus OX=10116 GN=Uncx PE=2 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 1.460e-40
Identity = 71/111 (63.96%), Postives = 80/111 (72.07%), Query Frame = 2

Query:  518 CKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISP 850
            C +  E   F++ D  D +      KRRRTRTNF  WQL++LE  F  SHYPDV  RE+LA  L L ESRVQVWFQNRRAKWRKKENTKKGPGRPAHN+ P TCSGEP+ P
Sbjct:   83 CGVAGESQPFKLADSGDPDKESPGCKRRRTRTNFTGWQLEELEKAFNESHYPDVFMREALAVRLDLVESRVQVWFQNRRAKWRKKENTKKGPGRPAHNSHPTTCSGEPMDP 193

[Close]

BLAST of DNA-binding protein vs. UniProt/SwissProt
Match: sp|A6NJT0|UNC4_HUMAN (Homeobox protein unc-4 homolog OS=Homo sapiens OX=9606 GN=UNCX PE=3 SV=1)

HSP 1 Score: 153.68 bits (387), Expect = 4.949e-40
Identity = 70/111 (63.06%), Postives = 80/111 (72.07%), Query Frame = 2

Query:  518 CKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISP 850
            C +  +   F++ D  D +      KRRRTRTNF  WQL++LE  F  SHYPDV  RE+LA  L L ESRVQVWFQNRRAKWRKKENTKKGPGRPAHN+ P TCSGEP+ P
Sbjct:   79 CGVGGDGQPFKLSDSGDPDKESPGCKRRRTRTNFTGWQLEELEKAFNESHYPDVFMREALALRLDLVESRVQVWFQNRRAKWRKKENTKKGPGRPAHNSHPTTCSGEPMDP 189

[Close]

BLAST of DNA-binding protein vs. UniProt/SwissProt
Match: sp|Q50D79|UNC4_DANRE (Homeobox protein unc-4 homolog OS=Danio rerio OX=7955 GN=uncx PE=2 SV=2)

HSP 1 Score: 148.673 bits (374), Expect = 1.015e-38
Identity = 70/112 (62.50%), Postives = 80/112 (71.43%), Query Frame = 2

Query:  515 NCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISP 850
             C +   D Q+++ D  D +      KRRRTRTNF  WQL++LE     SHYPDV  RE+LA  L L ESRVQVWFQNRRAKWRKKENTKKGPGRPAHN+ P TCSGEP+ P
Sbjct:   75 GCGVNG-DSQYKLGDGGDPDKESPGCKRRRTRTNFTGWQLEELEKASNESHYPDVFMREALALRLDLVESRVQVWFQNRRAKWRKKENTKKGPGRPAHNSHPTTCSGEPMDP 185

[Close]

BLAST of DNA-binding protein vs. UniProt/SwissProt
Match: sp|O77215|UNC4_DROME (Homeobox protein unc-4 OS=Drosophila melanogaster OX=7227 GN=unc-4 PE=2 SV=2)

HSP 1 Score: 136.732 bits (343), Expect = 8.287e-34
Identity = 63/86 (73.26%), Postives = 70/86 (81.40%), Query Frame = 2

Query:  593 KRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISP 850
            KRRR+RTNF  WQL++LE  F  SHYPD+  RE+LA  L L ESRV VWFQNRRAK RK+E+TKKGPGRPAHNAQP TCSGEPI P
Sbjct:  242 KRRRSRTNFNSWQLEELERAFSASHYPDIFMREALAMRLDLKESRVAVWFQNRRAKVRKREHTKKGPGRPAHNAQPQTCSGEPIPP 327

[Close]

BLAST of DNA-binding protein vs. TrEMBL
Match: O46118 (DNA-binding protein OS=Girardia tigrina OX=6162 GN=Dtprd-1 PE=2 SV=1)

HSP 1 Score: 576.244 bits (1484), Expect = 0.000e+0
Identity = 321/378 (84.92%), Postives = 344/378 (91.01%), Query Frame = 2

Query:  128 MDLSIENKYIGQLPTANNPTRTTNSFDSFVPGFPAMFNNLVGFSRYRLPRIVNSDQNFMPSLRFPNNAFLQSSTFDWINQTIPQCFMKDLMNNKTNSDSQIKLNGLANFPISFAPKISFSSTNNSSQLDNCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISPTXXXXXXXXXXXXXXXXXXXXXXXXAEKRKNSQXXXXXXXXXXXXXXXDRCNLAMKLQDSAGLFAHRSTDANSYLSPRFSGSDLLLPFPGDKIPELSQIKNLSEPLINRHKSYNINDILNSNAADVKNELSDCIDLS 1261
            MDLSIENKYIG L  ++N TR +N +++FVPGFPAMFNNLVGFSRYRLPRIVN+DQNFM SLRFPNNAFLQSST DWINQ IPQ  MKD++NNKTNSD  IKLNGL NFPISFAPKI+F+ TNNS+Q++NCKIKSE+FQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISPTELKQKELQRQERKRRKQEERNRKLAEKRKNSQ SLTISPSLSH+ST+DRCNLAMKLQDS  +F H ST+  SYLS RF+GSDLLL F  DKI  L+QIK L EPL NRHKSYNINDILNSNA D KN+LSD I+LS
Sbjct:    1 MDLSIENKYIGPLSASSNSTRPSNCYETFVPGFPAMFNNLVGFSRYRLPRIVNNDQNFMQSLRFPNNAFLQSSTIDWINQAIPQYIMKDMINNKTNSDPSIKLNGLGNFPISFAPKINFNGTNNSNQMENCKIKSEEFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISPTELKQKELQRQERKRRKQEERNRKLAEKRKNSQQSLTISPSLSHTSTVDRCNLAMKLQDSNNMFPHGSTETASYLSTRFNGSDLLLSFQNDKINSLNQIKTLPEPLCNRHKSYNINDILNSNATDAKNDLSDSINLS 378

[Close]

BLAST of DNA-binding protein vs. TrEMBL
Match: Q4RHA0 (Chromosome 3 SCAF15050, whole genome shotgun sequence (Fragment) OS=Tetraodon nigroviridis OX=99883 GN=GSTENG00034439001 PE=4 SV=1)

HSP 1 Score: 153.68 bits (387), Expect = 2.223e-40
Identity = 74/124 (59.68%), Postives = 88/124 (70.97%), Query Frame = 2

Query:  485 SSTNNSSQL--DNCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISP 850
            +S  NS+ L    C +  ++  +++ D  D +      KRRRTRTNF  WQL++LE  F  SHYPDV  RE+LA  L L ESRVQVWFQNRRAKWRKKENTKKGPGRPAHNA P TCSGEP++P
Sbjct:   60 ASVGNSNPLLSSGCGMNGDNQHYKLTDSGDPDKDSPGCKRRRTRTNFTGWQLEELEKAFNESHYPDVFMREALALRLDLIESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAHPTTCSGEPMAP 183

[Close]

BLAST of DNA-binding protein vs. TrEMBL
Match: A0A1U7SWL6 (LOW QUALITY PROTEIN: homeobox protein unc-4 homolog OS=Alligator sinensis OX=38654 GN=UNCX PE=4 SV=2)

HSP 1 Score: 152.14 bits (383), Expect = 6.875e-40
Identity = 70/112 (62.50%), Postives = 80/112 (71.43%), Query Frame = 2

Query:  515 NCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISP 850
             C +  +   F++ D  D +      KRRRTRTNF  WQL++LE  F  SHYPDV  RE+LA  L L ESRVQVWFQNRRAKWRKKENTKKGPGRPAHN+ P TCSGEP+ P
Sbjct:   73 GCGVSGDSQAFKLSDAGDPDKESPGCKRRRTRTNFTGWQLEELEKAFNESHYPDVFMREALALRLDLVESRVQVWFQNRRAKWRKKENTKKGPGRPAHNSHPTTCSGEPMDP 184

[Close]

BLAST of DNA-binding protein vs. TrEMBL
Match: Q4SMM1 (Chromosome 18 SCAF14547, whole genome shotgun sequence (Fragment) OS=Tetraodon nigroviridis OX=99883 GN=GSTENG00015693001 PE=4 SV=1)

HSP 1 Score: 151.369 bits (381), Expect = 7.041e-40
Identity = 73/123 (59.35%), Postives = 87/123 (70.73%), Query Frame = 2

Query:  482 FSSTNNSSQLDNCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISP 850
             S + ++S +    + + D QF++ D  D +      KRRRTRTNF  WQL++LE  F  SHYPDV  RE+LA  L L ESRVQVWFQNRRAKWRKKENTKKGPGRPAHN+ P TCSGEP+ P
Sbjct:   35 LSGSCSASVVAATPLLTPDSQFKLSDSGDPDKDSPGCKRRRTRTNFTGWQLEELEKAFNESHYPDVFMREALALRLDLVESRVQVWFQNRRAKWRKKENTKKGPGRPAHNSHPTTCSGEPMDP 157

[Close]

BLAST of DNA-binding protein vs. TrEMBL
Match: A0A0A7EPG2 (UNC homeobox protein transcript variant 1 (Fragment) OS=Homo sapiens OX=9606 GN=UNCX PE=2 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 1.099e-39
Identity = 70/111 (63.06%), Postives = 80/111 (72.07%), Query Frame = 2

Query:  518 CKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISP 850
            C +  +   F++ D  D +      KRRRTRTNF  WQL++LE  F  SHYPDV  RE+LA  L L ESRVQVWFQNRRAKWRKKENTKKGPGRPAHN+ P TCSGEP+ P
Sbjct:   79 CGVGGDGQPFKLSDSGDPDKESPGCKRRRTRTNFTGWQLEELEKAFNESHYPDVFMREALALRLDLVESRVQVWFQNRRAKWRKKENTKKGPGRPAHNSHPTTCSGEPMDP 189

[Close]

BLAST of DNA-binding protein vs. Ensembl Cavefish
Match: uncx (UNC homeobox [Source:NCBI gene;Acc:103039623])

HSP 1 Score: 152.525 bits (384), Expect = 5.151e-41
Identity = 70/110 (63.64%), Postives = 80/110 (72.73%), Query Frame = 2

Query:  515 NCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPI 844
             C +  +  Q+++ D  D E      KRRRTRTNF  WQL++LE  F  SHYPDV  RE+LA  L L ESRVQVWFQNRRAKWRKKENTKKGPGRPAHN+ P TCSGEP+
Sbjct:   74 GCGMNGDSQQYKLTDSGDPEKDSPGCKRRRTRTNFTGWQLEELEKAFNESHYPDVFMREALALRLDLIESRVQVWFQNRRAKWRKKENTKKGPGRPAHNSHPTTCSGEPM 183

[Close]

BLAST of DNA-binding protein vs. Ensembl Cavefish
Match: shox (short stature homeobox protein [Source:NCBI gene;Acc:103042484])

HSP 1 Score: 97.0561 bits (240), Expect = 2.944e-22
Identity = 48/99 (48.48%), Postives = 66/99 (66.67%), Query Frame = 2

Query:  482 FSSTNNSSQLDNCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKEN 778
            FS+   +  +  CK K ED +      S++E    + K+RR+RTNF + QL++LE +F  +HYPD   RE L++ LGL E+RVQVWFQNRRAK RK+EN
Sbjct:   83 FSAARAAEGIYECKEKKEDVK------SEDEDAQGKLKQRRSRTNFTLEQLNELERLFDETHYPDAFMREELSQRLGLSEARVQVWFQNRRAKCRKQEN 175

[Close]

BLAST of DNA-binding protein vs. Ensembl Cavefish
Match: shox (short stature homeobox protein [Source:NCBI gene;Acc:103042484])

HSP 1 Score: 95.9005 bits (237), Expect = 8.079e-22
Identity = 48/99 (48.48%), Postives = 66/99 (66.67%), Query Frame = 2

Query:  482 FSSTNNSSQLDNCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKEN 778
            FS+   +  +  CK K ED +      S++E    + K+RR+RTNF + QL++LE +F  +HYPD   RE L++ LGL E+RVQVWFQNRRAK RK+EN
Sbjct:   83 FSAARAAEGIYECKEKKEDVK------SEDEDAQGKLKQRRSRTNFTLEQLNELERLFDETHYPDAFMREELSQRLGLSEARVQVWFQNRRAKCRKQEN 175

[Close]

BLAST of DNA-binding protein vs. Ensembl Cavefish
Match: rx2 (retinal homeobox protein Rx2-like [Source:NCBI gene;Acc:103040507])

HSP 1 Score: 91.2781 bits (225), Expect = 4.733e-20
Identity = 40/63 (63.49%), Postives = 48/63 (76.19%), Query Frame = 2

Query:  587 EEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKE 775
            ++K RR RT F  +QL +LE  F+ SHYPDV SRE LA  + LPE RVQVWFQNRRAKWR++E
Sbjct:  135 KKKHRRNRTTFTTYQLHELERAFEKSHYPDVYSREELAMKVNLPEVRVQVWFQNRRAKWRRQE 197

[Close]

BLAST of DNA-binding protein vs. Ensembl Cavefish
Match: rx1 (retinal homeobox protein Rx1-like [Source:NCBI gene;Acc:103032969])

HSP 1 Score: 90.8929 bits (224), Expect = 6.692e-20
Identity = 44/76 (57.89%), Postives = 51/76 (67.11%), Query Frame = 2

Query:  557 DESDNETVDTEE---KRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKE 775
            D    E  D E+   K RR RT F  +QL +LE  F+ SHYPDV SRE LA  + LPE RVQVWFQNRRAKWR++E
Sbjct:  126 DRKSPEQGDEEQPKKKHRRNRTTFTTYQLHELERAFEKSHYPDVYSREELAMKVNLPEVRVQVWFQNRRAKWRRQE 201

[Close]

BLAST of DNA-binding protein vs. Ensembl Sea Lamprey
Match: dmbx1b (diencephalon/mesencephalon homeobox 1b [Source:NCBI gene;Acc:550288])

HSP 1 Score: 78.9518 bits (193), Expect = 1.754e-16
Identity = 36/62 (58.06%), Postives = 46/62 (74.19%), Query Frame = 2

Query:  596 RRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENT 781
            +RR+RT F   QL+ LE  F+ +HYPDV  RE LA    LPE+RVQVWF+NRRAK+RKK+ +
Sbjct:   43 QRRSRTAFTAQQLEALEKTFQKTHYPDVVMRERLAMCTNLPEARVQVWFKNRRAKFRKKQRS 104

[Close]

BLAST of DNA-binding protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010761.1 (pep scaffold:Pmarinus_7.0:GL476415:928307:936594:-1 gene:ENSPMAG00000009746.1 transcript:ENSPMAT00000010761.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 70.8626 bits (172), Expect = 1.014e-15
Identity = 34/58 (58.62%), Postives = 41/58 (70.69%), Query Frame = 2

Query:  593 KRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWR 766
            K+RR RT F   QLD LE +F  + YPD+  RE +A  + LPESRVQVWF+NRRAK R
Sbjct:    5 KQRRERTTFTRAQLDVLEALFSKTRYPDIFMREEVALKINLPESRVQVWFKNRRAKCR 62

[Close]

BLAST of DNA-binding protein vs. Ensembl Sea Lamprey
Match: emx2 (empty spiracles homeobox 2 [Source:ZFIN;Acc:ZDB-GENE-990415-54])

HSP 1 Score: 60.077 bits (144), Expect = 1.860e-10
Identity = 31/67 (46.27%), Postives = 46/67 (68.66%), Query Frame = 2

Query:  593 KRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGP 793
            K +R RT F+  QL +LE  F+ +HY   S R+ LA SL L E++V+VWFQNRR K ++++  ++GP
Sbjct:  186 KPKRIRTAFSPSQLLRLEHAFEKNHYVVGSERKQLASSLSLTETQVKVWFQNRRTKHKRQKLEEEGP 252

[Close]

BLAST of DNA-binding protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001141.1 (pep scaffold:Pmarinus_7.0:GL478075:36995:38394:-1 gene:ENSPMAG00000001023.1 transcript:ENSPMAT00000001141.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.0694 bits (131), Expect = 4.812e-10
Identity = 32/93 (34.41%), Postives = 46/93 (49.46%), Query Frame = 2

Query:  593 KRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRK------KENTKKGPGRPAHNAQPVTCSGEPISPT 853
            K RR RT F+  QL +LE  F    Y     R  +A +L L E++V++WFQNRR KW++      +E    GP R     +P   +   +  T
Sbjct:    2 KCRRPRTAFSSQQLLELERHFSRQRYLSRPQRFQVASALMLTETQVKIWFQNRRMKWKRGKGGQLQEKQSLGPARNGQAGRPAPAAASAVITT 94

[Close]

BLAST of DNA-binding protein vs. Ensembl Sea Lamprey
Match: barhl2 (BarH-like homeobox 2 [Source:ZFIN;Acc:ZDB-GENE-050913-153])

HSP 1 Score: 55.0694 bits (131), Expect = 7.607e-10
Identity = 27/61 (44.26%), Postives = 39/61 (63.93%), Query Frame = 2

Query:  590 EKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKK 772
            +K R+ RT F+  QL QLE  F+   Y  V  R  LA SL L +++V+ W+QNRR KW+++
Sbjct:    3 KKPRKARTAFSDHQLTQLERSFERQKYLSVQDRMELASSLSLSDTQVKTWYQNRRTKWKRQ 63

[Close]

BLAST of DNA-binding protein vs. Ensembl Yeast
Match: PHO2 (Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p; relocalizes to the cytosol in response to hypoxia [Source:SGD;Acc:S000002264])

HSP 1 Score: 50.8322 bits (120), Expect = 2.846e-7
Identity = 25/76 (32.89%), Postives = 43/76 (56.58%), Query Frame = 2

Query:  596 RRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPV 823
            +R  RT      LD L+  F+ +  P +  R+ ++  +G+PE  V++WFQNRRAK RKK++       P+  ++ +
Sbjct:   77 QRPKRTRAKGEALDVLKRKFEINPTPSLVERKKISDLIGMPEKNVRIWFQNRRAKLRKKQHGSNKDTIPSSQSRDI 152

[Close]

BLAST of DNA-binding protein vs. Ensembl Nematostella
Match: EDO39160 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SAY2])

HSP 1 Score: 88.9669 bits (219), Expect = 1.004e-21
Identity = 40/61 (65.57%), Postives = 49/61 (80.33%), Query Frame = 2

Query:  593 KRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKE 775
            K+RR RT F  +QL++LE  FK SHYPDV +RE+L+  + LPE R+QVWFQNRRAKWRKKE
Sbjct:    4 KKRRHRTIFTNYQLEELEKAFKESHYPDVYARENLSLKIDLPEDRIQVWFQNRRAKWRKKE 64

[Close]

BLAST of DNA-binding protein vs. Ensembl Nematostella
Match: EDO31796 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SWY2])

HSP 1 Score: 86.6557 bits (213), Expect = 4.856e-21
Identity = 43/84 (51.19%), Postives = 57/84 (67.86%), Query Frame = 2

Query:  557 DESDNETVDT-----------EEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKE 775
            ++SD E VD+           + K+RR RT F  +QL++LE  F  +HYPDV +RE+LA  + L E+RVQVWFQNRRAKWRK+E
Sbjct:    7 EKSDEEAVDSNNELEGKDSTAKRKQRRYRTTFTSYQLEELERAFAKTHYPDVFTREALAVKIDLTEARVQVWFQNRRAKWRKRE 90

[Close]

BLAST of DNA-binding protein vs. Ensembl Nematostella
Match: EDO38926 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SBH9])

HSP 1 Score: 88.9669 bits (219), Expect = 8.292e-21
Identity = 41/78 (52.56%), Postives = 55/78 (70.51%), Query Frame = 2

Query:  560 ESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGP 793
            +SD+     + K+RR RT F  +QL+++E VF+ +HYPDV +RE LA    L E+RVQVWFQNRRAKWRK+E   + P
Sbjct:    2 DSDSGDSAPKRKKRRNRTTFTAYQLEEMERVFQKTHYPDVYTREQLALRCALTEARVQVWFQNRRAKWRKRERFTQVP 79

[Close]

BLAST of DNA-binding protein vs. Ensembl Nematostella
Match: EDO42147 (Predicted protein; Retinal homeobox [Source:UniProtKB/TrEMBL;Acc:A7S2G8])

HSP 1 Score: 89.3521 bits (220), Expect = 3.647e-20
Identity = 41/73 (56.16%), Postives = 55/73 (75.34%), Query Frame = 2

Query:  557 DESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKE 775
            DE+D ++  +++K RR RT F  +QL +LE  F+ SHYPDV +RE LA  + LPE RVQVWFQN+RAKWR++E
Sbjct:   74 DEADGDS--SKKKLRRNRTTFTTFQLHELERAFEKSHYPDVYTREELALKISLPEVRVQVWFQNKRAKWRREE 144

[Close]

BLAST of DNA-binding protein vs. Ensembl Nematostella
Match: EDO42103 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2G7])

HSP 1 Score: 86.6557 bits (213), Expect = 9.909e-20
Identity = 43/81 (53.09%), Postives = 53/81 (65.43%), Query Frame = 2

Query:  551 IKDESDNETVDTEE----KRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENT 781
            +KD+   E    E+    K RR+RT F  +QL QLE  F+ + YPDV +RE LA  L L E+RVQVWFQNRRAKWRK+E  
Sbjct:    1 MKDDGSGEESAGEDGKPRKVRRSRTTFTTYQLHQLERAFEKTQYPDVFTREELALRLDLSEARVQVWFQNRRAKWRKREKA 81

[Close]

BLAST of DNA-binding protein vs. Ensembl Medaka
Match: uncx (UNC homeobox [Source:NCBI gene;Acc:101155113])

HSP 1 Score: 157.147 bits (396), Expect = 1.234e-42
Identity = 76/124 (61.29%), Postives = 88/124 (70.97%), Query Frame = 2

Query:  485 SSTNNSSQL--DNCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISP 850
            +S  NS+ L    C +  E+ Q+++ D  D +      KRRRTRTNF  WQL++LE  F  SHYPDV  RE+LA  L L ESRVQVWFQNRRAKWRKKENTKKGPGRPAHNA P TCSGEP+ P
Sbjct:   61 ASVGNSNPLLSSGCGMNGENQQYKLTDSGDPDKDSPGCKRRRTRTNFTGWQLEELEKAFNESHYPDVFMREALALRLDLIESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAHPTTCSGEPMDP 184

[Close]

BLAST of DNA-binding protein vs. Ensembl Medaka
Match: uncx4.1 (homeobox protein unc-4 homolog [Source:NCBI gene;Acc:101169335])

HSP 1 Score: 151.754 bits (382), Expect = 2.184e-40
Identity = 71/109 (65.14%), Postives = 82/109 (75.23%), Query Frame = 2

Query:  524 IKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISP 850
            + S+  QF++ D  D +  +   KRRRTRTNF  WQL++LE  F  SHYPDV  RE+LA  L L ESRVQVWFQNRRAKWRKKENTKKGPGRPAHN+ P TCSGEP+ P
Sbjct:   75 LSSDSQQFKLSDSGDPDKDNPGCKRRRTRTNFTGWQLEELEKAFNESHYPDVFMREALALRLDLVESRVQVWFQNRRAKWRKKENTKKGPGRPAHNSHPTTCSGEPMDP 183

[Close]

BLAST of DNA-binding protein vs. Ensembl Medaka
Match: mog-12 (Medaka OG-12 [Source:NCBI gene;Acc:100049320])

HSP 1 Score: 93.9745 bits (232), Expect = 3.101e-21
Identity = 47/99 (47.47%), Postives = 66/99 (66.67%), Query Frame = 2

Query:  482 FSSTNNSSQLDNCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKEN 778
            F+ +  +  +  CK K ED +      S++E    + K+RR+RTNF + QL++LE +F  +HYPD   RE L++ LGL E+RVQVWFQNRRAK RK+EN
Sbjct:   75 FNVSRATEGIYECKEKKEDVK------SEDEDAQGKLKQRRSRTNFTLEQLNELERLFDETHYPDAFMREELSQRLGLSEARVQVWFQNRRAKCRKQEN 167

[Close]

BLAST of DNA-binding protein vs. Ensembl Medaka
Match: rax (retina and anterior neural fold homeobox [Source:NCBI gene;Acc:100125459])

HSP 1 Score: 93.5893 bits (231), Expect = 4.233e-21
Identity = 48/88 (54.55%), Postives = 60/88 (68.18%), Query Frame = 2

Query:  512 DNCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKE 775
            D+C   S D   ++   SD+ET   ++K RR RT F  +QL +LE  F+ SHYPDV SRE LA  + LPE RVQVWFQNRRAKWR++E
Sbjct:   80 DSCAPDSPDRDGKL---SDDET--QKKKHRRNRTTFTTYQLHELERAFEKSHYPDVYSREELALKVNLPEVRVQVWFQNRRAKWRRQE 162

[Close]

BLAST of DNA-binding protein vs. Ensembl Medaka
Match: mog-12 (Medaka OG-12 [Source:NCBI gene;Acc:100049320])

HSP 1 Score: 93.2041 bits (230), Expect = 7.691e-21
Identity = 47/99 (47.47%), Postives = 66/99 (66.67%), Query Frame = 2

Query:  482 FSSTNNSSQLDNCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKEN 778
            F+ +  +  +  CK K ED +      S++E    + K+RR+RTNF + QL++LE +F  +HYPD   RE L++ LGL E+RVQVWFQNRRAK RK+EN
Sbjct:   75 FNVSRATEGIYECKEKKEDVK------SEDEDAQGKLKQRRSRTNFTLEQLNELERLFDETHYPDAFMREELSQRLGLSEARVQVWFQNRRAKCRKQEN 167

[Close]

BLAST of DNA-binding protein vs. Planmine SMEST
Match: SMESG000005018.1 (SMESG000005018.1)

HSP 1 Score: 696.041 bits (1795), Expect = 0.000e+0
Identity = 381/382 (99.74%), Postives = 381/382 (99.74%), Query Frame = 2

Query:  128 MDLSIENKYIGQLPTANNPTRTTNSFDSFVPGFPAMFNNLVGFSRYRLPRIVNSDQNFMPSLRFPNNAFLQSSTFDWINQTIPQCFMKDLMNNKTNSDSQIKLNGLANFPISFAPKISFSSTNNSSQLDNCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISPTXXXXXXXXXXXXXXXXXXXXXXXXAEKRKNSQXXXXXXXXXXXXXXXDRCNLAMKLQDSAGLFAHRSTDANSYLSPRFSGSDLLLPFPGDKIPELSQIKNLSEPLINRHKSYNINDILNSNAADVKNELSDCIDLSENQV 1273
            MDLSIENKYIGQLPTANNPTRTTNSFDSFVPGFPAMFNNLVGFSRYRLPRIVNSDQNFMPSLRFPNNAFLQSSTFDWINQTIPQCFMKDLMNNKTNSDSQIKLNGLANFPISFAPKISFSSTNNSSQLDNCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISPTELKQKELQRQERKRRKQEERNRKLAEKRKNSQ SLTISPSLSHSSTIDRCNLAMKLQDSAGLFAHRSTDANSYLSPRFSGSDLLLPFPGDKIPELSQIKNLSEPLINRHKSYNINDILNSNAADVKNELSDCIDLSENQV
Sbjct:    1 MDLSIENKYIGQLPTANNPTRTTNSFDSFVPGFPAMFNNLVGFSRYRLPRIVNSDQNFMPSLRFPNNAFLQSSTFDWINQTIPQCFMKDLMNNKTNSDSQIKLNGLANFPISFAPKISFSSTNNSSQLDNCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISPTELKQKELQRQERKRRKQEERNRKLAEKRKNSQQSLTISPSLSHSSTIDRCNLAMKLQDSAGLFAHRSTDANSYLSPRFSGSDLLLPFPGDKIPELSQIKNLSEPLINRHKSYNINDILNSNAADVKNELSDCIDLSENQV 382

[Close]

BLAST of DNA-binding protein vs. Planmine SMEST
Match: SMESG000020038.1 (SMESG000020038.1)

HSP 1 Score: 99.7525 bits (247), Expect = 1.166e-22
Identity = 47/87 (54.02%), Postives = 64/87 (73.56%), Query Frame = 2

Query:  524 IKSEDFQFQIKDESDNETVDTEE---KRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKE 775
            ++S   + Q   +SDN + ++EE   K+RR RT F+ +QLD++E +F+ +HYPDV +RE LA   GL E+RVQVWFQNRRAKWRKKE
Sbjct:   38 MRSAPEEIQSCSKSDNGSANSEEAKKKKRRNRTTFSSYQLDEMERIFQKTHYPDVYAREQLAFRTGLSEARVQVWFQNRRAKWRKKE 124

[Close]

BLAST of DNA-binding protein vs. Planmine SMEST
Match: SMESG000033843.1 (SMESG000033843.1)

HSP 1 Score: 94.3597 bits (233), Expect = 1.095e-21
Identity = 45/78 (57.69%), Postives = 52/78 (66.67%), Query Frame = 2

Query:  590 EKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKKGPGRPAHNAQPV 823
            +K RR RT F  +QL +LE  F+ SHYPDV SRE LA  + LPE RVQVWFQNRRAKWR++E  K       HN Q V
Sbjct:   88 KKHRRNRTTFTTYQLHELERAFEKSHYPDVYSREELAMKISLPEVRVQVWFQNRRAKWRRQE--KIEAANHTHNLQEV 163

[Close]

BLAST of DNA-binding protein vs. Planmine SMEST
Match: SMESG000039056.1 (SMESG000039056.1)

HSP 1 Score: 93.9745 bits (232), Expect = 2.193e-20
Identity = 45/108 (41.67%), Postives = 70/108 (64.81%), Query Frame = 2

Query:  464 FAPKISFSSTNNSSQLDNCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKENTKK 787
            ++  I+ S +N SS+  N      ++   +K E+D+  +  + K +R RT+F+  QLD LE  F+ +HYPDV +RE LA  + LPE+R+QVWF NRRAKWR++E  ++
Sbjct:  248 YSESIAHSESNASSEPGN------EYMSGVKSENDDMRIKLKRKLQRNRTSFSTDQLDSLEKEFERTHYPDVFAREKLADKISLPEARIQVWFSNRRAKWRREEKLRR 349

[Close]

BLAST of DNA-binding protein vs. Planmine SMEST
Match: SMESG000080420.1 (SMESG000080420.1)

HSP 1 Score: 92.4337 bits (228), Expect = 4.774e-20
Identity = 50/125 (40.00%), Postives = 73/125 (58.40%), Query Frame = 2

Query:  413 NSDSQIKLNGLANFPISFAPKISFSSTNN----SSQLDNCKIKSEDFQFQIKDESDNETVDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESRVQVWFQNRRAKWRKKE 775
            NS++++ +N     P+   P  + S+  N     S L+N   +   F   + D+     + +  K+RR RT F + QL++LE  F+ +HYPDV +RE LA  + L E+RVQVWFQNRRAKWRK E
Sbjct:   85 NSNTEVSINN----PLPAPPNSALSTARNPSLSYSNLNNNYYQHYGFHHDLADD-----IFSRRKQRRNRTTFTMQQLEELEKAFQQTHYPDVFTREELALRISLTEARVQVWFQNRRAKWRKTE 200

The following BLAST results are available for this feature:

BLAST of DNA-binding protein vs. Ensembl Human
BLAST of DNA-binding protein vs. Ensembl Celegans
BLAST of DNA-binding protein vs. Ensembl Fly
BLAST of DNA-binding protein vs. Ensembl Zebrafish
BLAST of DNA-binding protein vs. Ensembl Xenopus
BLAST of DNA-binding protein vs. Ensembl Mouse
BLAST of DNA-binding protein vs. UniProt/SwissProt
BLAST of DNA-binding protein vs. TrEMBL
BLAST of DNA-binding protein vs. Ensembl Cavefish
BLAST of DNA-binding protein vs. Ensembl Sea Lamprey
BLAST of DNA-binding protein vs. Ensembl Yeast
BLAST of DNA-binding protein vs. Ensembl Nematostella
BLAST of DNA-binding protein vs. Ensembl Medaka
BLAST of DNA-binding protein vs. Planmine SMEST

BLAST of DNA-binding protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
UNCX	1.030e-40	63.06	UNC homeobox [Source:HGNC Symbol;Acc:HGNC:33194]	[more]
RAX2	9.684e-22	61.43	retina and anterior neural fold homeobox 2 [Source...	[more]
RAX2	9.684e-22	61.43	retina and anterior neural fold homeobox 2 [Source...	[more]
SHOX	1.075e-21	48.48	short stature homeobox [Source:HGNC Symbol;Acc:HGN...	[more]
SHOX	1.075e-21	48.48	short stature homeobox [Source:HGNC Symbol;Acc:HGN...	[more]

BLAST of DNA-binding protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
unc-4	6.717e-27	58.06	Homeobox protein unc-4 [Source:UniProtKB/Swiss-Pr...	[more]
alr-1	4.672e-19	41.79	AristaLess (Drosophila homeodomain) Related [Sour...	[more]
ceh-8	2.268e-18	58.73	Homeobox protein ceh-8 [Source:UniProtKB/Swiss-Pr...	[more]
ceh-10	2.749e-18	57.58	Homeobox protein ceh-10 [Source:UniProtKB/Swiss-P...	[more]
ceh-8	4.259e-18	58.73	Homeobox protein ceh-8 [Source:UniProtKB/Swiss-Pr...	[more]

BLAST of DNA-binding protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
unc-4	8.267e-35	73.26	gene:FBgn0024184 transcript:FBtr0340626	[more]
unc-4	8.267e-35	73.26	gene:FBgn0024184 transcript:FBtr0340627	[more]
OdsH	1.576e-34	69.05	gene:FBgn0026058 transcript:FBtr0074461	[more]
Rx	2.672e-21	43.41	gene:FBgn0020617 transcript:FBtr0342783	[more]
al	1.767e-19	61.19	gene:FBgn0000061 transcript:FBtr0078053	[more]

BLAST of DNA-binding protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
uncx	3.854e-41	62.50	UNC homeobox [Source:ZFIN;Acc:ZDB-GENE-080509-1]	[more]
uncx4.1	7.794e-41	63.39	Unc4.1 homeobox (C. elegans) [Source:NCBI gene;Acc...	[more]
shox	5.460e-23	50.51	short stature homeobox [Source:ZFIN;Acc:ZDB-GENE-0...	[more]
shox2	8.106e-21	44.34	short stature homeobox 2 [Source:ZFIN;Acc:ZDB-GENE...	[more]
rx2	1.406e-20	47.42	retinal homeobox gene 2 [Source:NCBI gene;Acc:3047...	[more]

BLAST of DNA-binding protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
uncx	6.393e-41	62.50	UNC homeobox [Source:NCBI gene;Acc:100492016]	[more]
SHOX	1.791e-21	49.49	short stature homeobox [Source:NCBI gene;Acc:10048...	[more]
ENSXETT00000028778.1	1.069e-20	60.00	retina and anterior neural fold homeobox [Source:N...	[more]
gja10	1.403e-20	42.70	gap junction protein alpha 10 [Source:Xenbase;Acc:...	[more]
ENSXETT00000045475.1	2.239e-20	61.76	pep primary_assembly:Xenopus_tropicalis_v9.1:8:382...	[more]

BLAST of DNA-binding protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Uncx	1.575e-41	63.96	UNC homeobox [Source:MGI Symbol;Acc:MGI:108013]	[more]
Alx3	4.853e-21	58.33	aristaless-like homeobox 3 [Source:MGI Symbol;Acc:...	[more]
Alx3	2.255e-20	58.33	aristaless-like homeobox 3 [Source:MGI Symbol;Acc:...	[more]
Shox2	5.308e-20	61.54	short stature homeobox 2 [Source:MGI Symbol;Acc:MG...	[more]
Rax	5.670e-20	56.76	retina and anterior neural fold homeobox [Source:M...	[more]

BLAST of DNA-binding protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|O08934\|UNC4_MOUSE	1.102e-40	63.96	Homeobox protein unc-4 homolog OS=Mus musculus OX=...	[more]
sp\|P97830\|UNC4_RAT	1.460e-40	63.96	Homeobox protein unc-4 homolog OS=Rattus norvegicu...	[more]
sp\|A6NJT0\|UNC4_HUMAN	4.949e-40	63.06	Homeobox protein unc-4 homolog OS=Homo sapiens OX=...	[more]
sp\|Q50D79\|UNC4_DANRE	1.015e-38	62.50	Homeobox protein unc-4 homolog OS=Danio rerio OX=7...	[more]
sp\|O77215\|UNC4_DROME	8.287e-34	73.26	Homeobox protein unc-4 OS=Drosophila melanogaster ...	[more]

BLAST of DNA-binding protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
O46118	0.000e+0	84.92	DNA-binding protein OS=Girardia tigrina OX=6162 GN...	[more]
Q4RHA0	2.223e-40	59.68	Chromosome 3 SCAF15050, whole genome shotgun seque...	[more]
A0A1U7SWL6	6.875e-40	62.50	LOW QUALITY PROTEIN: homeobox protein unc-4 homolo...	[more]
Q4SMM1	7.041e-40	59.35	Chromosome 18 SCAF14547, whole genome shotgun sequ...	[more]
A0A0A7EPG2	1.099e-39	63.06	UNC homeobox protein transcript variant 1 (Fragmen...	[more]

BLAST of DNA-binding protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
uncx	5.151e-41	63.64	UNC homeobox [Source:NCBI gene;Acc:103039623]	[more]
shox	2.944e-22	48.48	short stature homeobox protein [Source:NCBI gene;A...	[more]
shox	8.079e-22	48.48	short stature homeobox protein [Source:NCBI gene;A...	[more]
rx2	4.733e-20	63.49	retinal homeobox protein Rx2-like [Source:NCBI gen...	[more]
rx1	6.692e-20	57.89	retinal homeobox protein Rx1-like [Source:NCBI gen...	[more]

BLAST of DNA-binding protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
dmbx1b	1.754e-16	58.06	diencephalon/mesencephalon homeobox 1b [Source:NCB...	[more]
ENSPMAT00000010761.1	1.014e-15	58.62	pep scaffold:Pmarinus_7.0:GL476415:928307:936594:-...	[more]
emx2	1.860e-10	46.27	empty spiracles homeobox 2 [Source:ZFIN;Acc:ZDB-GE...	[more]
ENSPMAT00000001141.1	4.812e-10	34.41	pep scaffold:Pmarinus_7.0:GL478075:36995:38394:-1 ...	[more]
barhl2	7.607e-10	44.26	BarH-like homeobox 2 [Source:ZFIN;Acc:ZDB-GENE-050...	[more]

BLAST of DNA-binding protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
PHO2	2.846e-7	32.89	Homeobox transcription factor; regulatory targets ...	[more]

BLAST of DNA-binding protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO39160	1.004e-21	65.57	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO31796	4.856e-21	51.19	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO38926	8.292e-21	52.56	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO42147	3.647e-20	56.16	Predicted protein; Retinal homeobox [Source:UniPr...	[more]
EDO42103	9.909e-20	53.09	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of DNA-binding protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
uncx	1.234e-42	61.29	UNC homeobox [Source:NCBI gene;Acc:101155113]	[more]
uncx4.1	2.184e-40	65.14	homeobox protein unc-4 homolog [Source:NCBI gene;A...	[more]
mog-12	3.101e-21	47.47	Medaka OG-12 [Source:NCBI gene;Acc:100049320]	[more]
rax	4.233e-21	54.55	retina and anterior neural fold homeobox [Source:N...	[more]
mog-12	7.691e-21	47.47	Medaka OG-12 [Source:NCBI gene;Acc:100049320]	[more]

BLAST of DNA-binding protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000005018.1	0.000e+0	99.74	SMESG000005018.1	[more]
SMESG000020038.1	1.166e-22	54.02	SMESG000020038.1	[more]
SMESG000033843.1	1.095e-21	57.69	SMESG000033843.1	[more]
SMESG000039056.1	2.193e-20	41.67	SMESG000039056.1	[more]
SMESG000080420.1	4.774e-20	40.00	SMESG000080420.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30032043 ID=SMED30032043|Name=DNA-binding protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1294bp
AGTCGAAAGCGTTTTTTATTGTCTGGTTAGTTCAGAAAGTCAACTCTGAT
TGGTCAATGATAAATAATAAAATCAAATCTAATTATTAAAGTAATCTTTG
ATTAATTTCATTATCACATGAATAAAAATGGATTTGTCAATTGAAAACAA
ATATATCGGACAATTACCGACTGCGAATAATCCAACGAGAACGACAAATT
CTTTCGACTCATTTGTCCCAGGATTTCCAGCAATGTTCAACAATCTCGTC
GGGTTTTCTCGATATCGGTTGCCTCGAATTGTCAATTCCGATCAGAATTT
CATGCCATCTTTGAGATTCCCAAATAACGCATTTCTCCAATCATCGACTT
TCGACTGGATCAATCAAACGATTCCTCAGTGTTTCATGAAAGATCTAATG
AACAATAAAACAAATTCAGACTCACAGATTAAACTCAACGGTTTGGCGAA
TTTCCCAATATCATTCGCTCCGAAAATAAGTTTCAGCAGCACCAACAATT
CCAGTCAACTTGACAATTGTAAAATCAAATCTGAGGACTTTCAATTTCAA
ATCAAAGATGAAAGCGATAATGAAACAGTAGATACAGAAGAAAAAAGAAG
ACGAACAAGAACGAATTTCGCTATTTGGCAATTAGATCAATTGGAAGTTG
TGTTTAAAACAAGTCACTATCCAGATGTCAGTTCTCGAGAGAGTTTAGCC
CGATCATTGGGTTTACCAGAATCTCGAGTTCAGGTTTGGTTTCAGAATCG
TCGAGCTAAATGGCGGAAAAAAGAAAACACAAAAAAAGGGCCAGGAAGAC
CTGCTCATAATGCTCAACCTGTAACGTGTTCCGGGGAGCCAATATCGCCA
ACGGAATTAAAACAAAAAGAGTTACAGAGGCAGGAAAGAAAACGTCGTAA
ACAAGAAGAACGCAACCGAAAATTAGCAGAAAAACGGAAAAATTCCCAAC
ATTCTCTGACAATTTCTCCTTCTCTTTCCCACAGTTCAACAATCGACAGA
TGCAACCTTGCCATGAAACTACAAGACTCGGCTGGCTTATTTGCCCACCG
ATCAACAGATGCAAATTCTTATTTATCCCCAAGATTTTCCGGATCAGATC
TATTATTGCCGTTCCCCGGTGACAAAATTCCCGAATTATCTCAAATTAAA
AACCTATCCGAACCACTCATTAATCGACACAAATCCTACAATATTAACGA
CATTCTCAATTCCAATGCTGCCGATGTCAAAAATGAGCTTTCAGACTGTA
TCGATTTGTCTGAGAATCAAGTGTAAATATATCGATTTTTATTC

protein sequence of SMED30032043-orf-1

>SMED30032043-orf-1 ID=SMED30032043-orf-1|Name=SMED30032043-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=383bp
MDLSIENKYIGQLPTANNPTRTTNSFDSFVPGFPAMFNNLVGFSRYRLPR
IVNSDQNFMPSLRFPNNAFLQSSTFDWINQTIPQCFMKDLMNNKTNSDSQ
IKLNGLANFPISFAPKISFSSTNNSSQLDNCKIKSEDFQFQIKDESDNET
VDTEEKRRRTRTNFAIWQLDQLEVVFKTSHYPDVSSRESLARSLGLPESR
VQVWFQNRRAKWRKKENTKKGPGRPAHNAQPVTCSGEPISPTELKQKELQ
RQERKRRKQEERNRKLAEKRKNSQHSLTISPSLSHSSTIDRCNLAMKLQD
SAGLFAHRSTDANSYLSPRFSGSDLLLPFPGDKIPELSQIKNLSEPLINR
HKSYNINDILNSNAADVKNELSDCIDLSENQV*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **INTERPRO**
Term	Definition
IPR000047	HTH_motif
IPR001356	Homeobox_dom
IPR009057	Homeobox-like_sf
IPR017970	Homeobox_CS

Vocabulary: **molecular function**
Term	Definition
GO:0003677	DNA binding
GO:0043565	sequence-specific DNA binding

Vocabulary: **biological process**
Term	Definition
GO:0006355	regulation of transcription, DNA-templated

Vocabulary: **cellular component**
Term	Definition
GO:0005634	nucleus

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 242..277
None	No IPR available	GENE3D	G3DSA:1.10.10.60		coord: 142..219 e-value: 2.6E-27 score: 96.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 244..271
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 212..284
None	No IPR available	PANTHER	PTHR46799	FAMILY NOT NAMED	coord: 108..278
IPR000047	Helix-turn-helix motif	PRINTS	PR00031	HTHREPRESSR	coord: 186..195 score: 49.51 coord: 195..211 score: 57.51
IPR001356	Homeobox domain	SMART	SM00389	HOX_1	coord: 157..219 e-value: 3.1E-25 score: 99.8
IPR001356	Homeobox domain	PFAM	PF00046	Homeodomain	coord: 158..214 e-value: 5.3E-22 score: 77.4
IPR001356	Homeobox domain	PROSITE	PS50071	HOMEOBOX_2	coord: 155..215 score: 20.293
IPR001356	Homeobox domain	CDD	cd00086	homeodomain	coord: 158..216 e-value: 2.04281E-20 score: 81.906
IPR017970	Homeobox, conserved site	PROSITE	PS00027	HOMEOBOX_1	coord: 190..213
IPR009057	Homeobox-like domain superfamily	SUPERFAMILY	SSF46689	Homeodomain-like	coord: 149..216