SMED30031990

Overview
NameSMED30031990
Smed IDSMED30031990
Length (bp)1588
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30031990 (SMED30031990) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30031990 (SMED30031990) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30031990 (SMED30031990) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30031990 (SMED30031990) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30031990

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 19

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pigment cellSMED30031990SMESG000025001.1 SmedASXL_012073SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult RNA-sequencing evidence
X1 cellSMED30031990SMESG000025001.1 SmedASXL_012073SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30031990SMESG000025001.1 SmedASXL_012073SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
epidermisSMED30031990SMESG000025001.1 dd_Smed_v4_213_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30031990SMESG000025001.1 dd_Smed_v4_213_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30031990SMESG000025001.1 dd_Smed_v4_213_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031990SMESG000025001.1 dd_Smed_v4_213_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30031990SMESG000025001.1 dd_Smed_v4_213_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031990SMESG000025001.1 SMESG000025000.1 Contig28945uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031990SMESG000025001.1 SMESG000025000.1 Contig28945newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031990SMESG000025001.1 Contig15989uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031990SMESG000025001.1 Contig15989newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031990SMESG000025001.1 Contig35187uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031990SMESG000025001.1 Contig35187newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031990SMESG000025001.1 Contig13133uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031990SMESG000025001.1 Contig13133newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Category 2 cellSMED30031990SMESG000025001.1 dd_Smed_v6_213_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 2 cellSMED30031990SMESG000025001.1 dd_Smed_v4_213_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Category 2 cellSMED30031990 dd_Smed_v4_40742_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30031990 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.016160:8250..9878 +6957
v31.026265:3849..5240 -4872
Homology
[Close]
BLAST of SMED30031990 vs. TrEMBL
Match: A0A5N4B1W9 (Uncharacterized protein OS=Photinus pyralis OX=7054 GN=PPYR_00488 PE=4 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 4.219e-8
Identity = 55/166 (33.13%), Postives = 84/166 (50.60%), Query Frame = -3 
Query: 1128 FNDWQYTSENLSRHEKTLLNIENCTK*KNIE---LNLQKPVQ*IKICNDTMTPRKHDVTELSNV*YLWNN----------FLHDRVLALRGGSNKLYEKNNWNFLKSIEMIGKFDDIMANHINRVQKSISLKKKRLKN*YKPIVIIRKVPYPIIYFLSN*LIWQIV 1586
            ++DW++ S  LSRHEK+  +IE+  K  ++E   LNL            T+  ++  + E+  V Y W N          FL  + LA RG S KL+  NN NFL+ ++MI KFD +M+ H+ ++Q   + K K+      P  +  K    II  LSN +  QI+
Sbjct:  256 YSDWKHLSNTLSRHEKSTQHIESYKKWIDLEKRLLNLT-----------TIDSKEQRLLEM-QVKY-WQNVIERLIAIIQFLASQCLAFRGTSTKLFAHNNGNFLQCVQMISKFDPVMSEHLKKIQ---TFKNKK----SLPHYLGYKFQNEIISLLSNTIQMQII 401
[Close]
BLAST of SMED30031990 vs. TrEMBL
Match: K7UW31 (General transcription factor 2-related zinc finger protein OS=Zea mays OX=4577 GN=ZEAMMB73_Zm00001d050797 PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 8.403e-8
Identity = 37/113 (32.74%), Postives = 57/113 (50.44%), Query Frame = -3 
Query: 1248 FNDWQYTSENLSRHEKTLLNIENCTK*KNIELNLQKPVQ*IKICNDTMTPRKHDVTELSNV*YLWNNFLHDRVLALRGGSNKLYEKNNWNFLKSIEMIGKFDDIMANHINRVQ 1586
            F DW++TSE L  HE ++ +I +      + + L+K     K     +   K  + ++         FL  R LA RG S +LY   N NFL  +EMI +FD +M +H+ R+Q
Sbjct:   53 FGDWRHTSERLKEHEASMEHITSMNSWNELRVRLRKTETIDKEFQHQIAKEKERIRQVLLRIIAVVKFLGKRTLAFRGSSEQLYNDQNGNFLACLEMIAEFDVVMQDHLRRIQ 165
[Close]
BLAST of SMED30031990 vs. TrEMBL
Match: A0A5N4B294 (Uncharacterized protein OS=Photinus pyralis OX=7054 GN=PPYR_00480 PE=4 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 8.999e-8
Identity = 55/166 (33.13%), Postives = 84/166 (50.60%), Query Frame = -3 
Query: 1128 FNDWQYTSENLSRHEKTLLNIENCTK*KNIE---LNLQKPVQ*IKICNDTMTPRKHDVTELSNV*YLWNN----------FLHDRVLALRGGSNKLYEKNNWNFLKSIEMIGKFDDIMANHINRVQKSISLKKKRLKN*YKPIVIIRKVPYPIIYFLSN*LIWQIV 1586
            ++DW++ S  LSRHEK+  +IE+  K  ++E   LNL            T+  ++  + E+  V Y W N          FL  + LA RG S KL+  NN NFL+ ++MI KFD +M+ H+ ++Q   + K K+      P  +  K    II  LSN +  QI+
Sbjct:  256 YSDWKHLSNTLSRHEKSTQHIESYKKWIDLEKRLLNLT-----------TIDSKEQRLLEM-QVKY-WQNVIERLIAIIQFLASQCLAFRGTSTKLFAHNNGNFLQCVQMISKFDPVMSEHLKKIQ---TFKNKK----SLPHYLGYKFQNEIISLLSNTIQMQII 401
[Close]
BLAST of SMED30031990 vs. TrEMBL
Match: A0A1D6F7M2 (General transcription factor 2-related zinc finger protein OS=Zea mays OX=4577 GN=ZEAMMB73_Zm00001d007629 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 2.424e-7
Identity = 37/113 (32.74%), Postives = 57/113 (50.44%), Query Frame = -3 
Query: 1248 FNDWQYTSENLSRHEKTLLNIENCTK*KNIELNLQKPVQ*IKICNDTMTPRKHDVTELSNV*YLWNNFLHDRVLALRGGSNKLYEKNNWNFLKSIEMIGKFDDIMANHINRVQ 1586
            F DW++TSE L  HE ++ +I + +    + + L+K     K     +   K  + ++         FL  R LA RG S +LY   N NFL  +EMI +FD +M +H+ R Q
Sbjct:   92 FGDWRHTSERLKEHEASMEHITSMSSWNELRVRLRKTETIDKEFQHQIAKEKERIRQVLLRIIDVVKFLGKRTLAFRGSSEQLYNDQNGNFLACLEMIAEFDVVMQDHLRRTQ 204
[Close]
BLAST of SMED30031990 vs. TrEMBL
Match: A0A287PTU3 (TTF-type domain-containing protein OS=Hordeum vulgare subsp. vulgare OX=112509 PE=4 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 2.437e-7
Identity = 37/113 (32.74%), Postives = 58/113 (51.33%), Query Frame = -3 
Query: 1248 FNDWQYTSENLSRHEKTLLNIENCTK*KNIELNLQKPVQ*IKICNDTMTPRKHDVTELSNV*YLWNNFLHDRVLALRGGSNKLYEKNNWNFLKSIEMIGKFDDIMANHINRVQ 1586
            F DW++ SE L  HE ++ +I N      + + L K     K     +T  K  + ++         FL  R LA RG S +LY+ +N NFL  +EM+ +FD +M +H+ R+Q
Sbjct:  199 FCDWRHISERLKEHEASVDHITNMNSWNELRIRLSKHETIDKELQQQITKEKERIRQVLLRIVAIVKFLGKRNLAFRGSSEQLYDDSNGNFLACVEMVAEFDFVMQDHLRRIQ 311
[Close]
BLAST of SMED30031990 vs. Ensembl Cavefish
Match: ENSAMXT00000037907.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02002089.1:152797:155023:-1 gene:ENSAMXG00000037599.1 transcript:ENSAMXT00000037907.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.8322 bits (120), Expect = 3.352e-6
Identity = 22/46 (47.83%), Postives = 32/46 (69.57%), Query Frame = -3 
Query: 1245 LHDRVLALRGGSNKLYEKNNWNFLKSIEMIGKFDDIMANHINRVQK 1382
            L +R  ALRG S KL+E +N NFL  IE++ +FD +M+ H+ R+ K
Sbjct:  171 LAERNQALRGHSEKLFEPHNGNFLGQIELMAQFDPVMSEHLRRIDK 216
[Close]
BLAST of SMED30031990 vs. Ensembl Cavefish
Match: ENSAMXT00000044961.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000032.1:419978:423709:1 gene:ENSAMXG00000036481.1 transcript:ENSAMXT00000044961.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.447 bits (119), Expect = 4.328e-6
Identity = 21/45 (46.67%), Postives = 32/45 (71.11%), Query Frame = -3 
Query: 1248 LHDRVLALRGGSNKLYEKNNWNFLKSIEMIGKFDDIMANHINRVQ 1382
            L +R  ALRG +++LY+ +N NFL  +E++ KFD +M  HI R+Q
Sbjct:  224 LAERNQALRGTTSELYDPHNGNFLAQVELMAKFDPVMNEHIRRIQ 268
[Close]
BLAST of SMED30031990 vs. Ensembl Medaka
Match: ENSORLT00000028133.1 (zinc finger MYM-type protein 1-like [Source:NCBI gene;Acc:111948955])

HSP 1 Score: 50.447 bits (119), Expect = 4.509e-7
Identity = 21/41 (51.22%), Postives = 30/41 (73.17%), Query Frame = -3 
Query: 1251 RVLALRGGSNKLYEKNNWNFLKSIEMIGKFDDIMANHINRV 1373
            R LALRG +  L+  +N NFLK +E++ +FD IM +H+NRV
Sbjct:  284 RNLALRGHTETLFTSSNGNFLKEVELMARFDPIMKDHLNRV 324

HSP 2 Score: 23.0978 bits (48), Expect = 4.509e-7
Identity = 12/39 (30.77%), Postives = 21/39 (53.85%), Query Frame = -2 
Query: 1357 TIDKNMQRYNDAEKTRCYRIVERLISVEQFLA*QGFSFK 1473
            TIDK      + E+ R   ++ RLI++ Q LA +  + +
Sbjct:  251 TIDKQEMALLEEERVRWRAVLTRLIAIVQSLAVRNLALR 289
[Close]
BLAST of SMED30031990 vs. Ensembl Medaka
Match: ENSORLT00000042294.1 (zinc finger MYM-type protein 1-like [Source:NCBI gene;Acc:110016639])

HSP 1 Score: 50.447 bits (119), Expect = 4.509e-7
Identity = 21/41 (51.22%), Postives = 30/41 (73.17%), Query Frame = -3 
Query: 1251 RVLALRGGSNKLYEKNNWNFLKSIEMIGKFDDIMANHINRV 1373
            R LALRG +  L+  +N NFLK +E++ +FD IM +H+NRV
Sbjct:  284 RNLALRGHTETLFTSSNGNFLKEVELMARFDPIMKDHLNRV 324

HSP 2 Score: 23.0978 bits (48), Expect = 4.509e-7
Identity = 12/39 (30.77%), Postives = 21/39 (53.85%), Query Frame = -2 
Query: 1357 TIDKNMQRYNDAEKTRCYRIVERLISVEQFLA*QGFSFK 1473
            TIDK      + E+ R   ++ RLI++ Q LA +  + +
Sbjct:  251 TIDKQEMALLEEERVRWRAVLTRLIAIVQSLAVRNLALR 289
[Close]
BLAST of SMED30031990 vs. Ensembl Medaka
Match: ENSORLT00000045044.1 (zinc finger MYM-type protein 1-like [Source:NCBI gene;Acc:111948193])

HSP 1 Score: 50.447 bits (119), Expect = 4.509e-7
Identity = 21/41 (51.22%), Postives = 30/41 (73.17%), Query Frame = -3 
Query: 1251 RVLALRGGSNKLYEKNNWNFLKSIEMIGKFDDIMANHINRV 1373
            R LALRG +  L+  +N NFLK +E++ +FD IM +H+NRV
Sbjct:  284 RNLALRGHTETLFTSSNGNFLKEVELMARFDPIMKDHLNRV 324

HSP 2 Score: 23.0978 bits (48), Expect = 4.509e-7
Identity = 12/39 (30.77%), Postives = 21/39 (53.85%), Query Frame = -2 
Query: 1357 TIDKNMQRYNDAEKTRCYRIVERLISVEQFLA*QGFSFK 1473
            TIDK      + E+ R   ++ RLI++ Q LA +  + +
Sbjct:  251 TIDKQEMALLEEERVRWRAVLTRLIAIVQSLAVRNLALR 289
[Close]
BLAST of SMED30031990 vs. Planmine SMEST
Match: SMESG000025001.1 (SMESG000025001.1)

HSP 1 Score: 229.95 bits (585), Expect = 1.429e-73
Identity = 115/124 (92.74%), Postives = 122/124 (98.39%), Query Frame = 1 
Query:  343 MNKLLIFAFALLFVSIECRYKLVQELSKRELSESCQKELDKMNEDYDKAVAKIDSDCDAAFLKIQEPFAPLWLDFESRCGLDFDKMSEECIDEAKKLDDDYTKEIKKLDSSCDAEFAKTVENLS 714
            MNKLLIFAFALLFVSIECRYKLVQELSKRELSESCQKELDKMNEDYDKAVAKIDSDCDAAFLKI++PF+PL+LD ESRCGLD+DKMSEECIDE KKLDDD+TKEI+KLDSSCDAEFAKTVENLS
Sbjct:    1 MNKLLIFAFALLFVSIECRYKLVQELSKRELSESCQKELDKMNEDYDKAVAKIDSDCDAAFLKIEKPFSPLYLDVESRCGLDYDKMSEECIDETKKLDDDFTKEIEKLDSSCDAEFAKTVENLS 124
[Close]
BLAST of SMED30031990 vs. Planmine SMEST
Match: SMESG000054780.1 (SMESG000054780.1)

HSP 1 Score: 109.768 bits (273), Expect = 3.278e-28
Identity = 61/124 (49.19%), Postives = 81/124 (65.32%), Query Frame = 1 
Query:  343 MNKLLIFAFALLFVSIECRYKLVQ-ELSKRELSESCQKELDKMNEDYDKAVAKIDSDCDAAFLKIQEPFAPLWLDFESRCGLDFDKMSEECIDEAKKLDDDYTKEIKKLDSSCDAEFAKTVENL 711
            M   LI   A+LFVSIECRY+  + EL+KRELS  C KE++K++E  + A  KID+ C  A+  +    +  +L  E  CG   DKMS+EC  EAKKLDD ++KEI KLD SCD E+A  V+N+
Sbjct:    1 MKTFLIICLAILFVSIECRYRARRVELNKRELSAECSKEVEKLDEALNTATDKIDATCQKAYDDLVMKNSVEYLKIEKDCGNYIDKMSKECYAEAKKLDDSFSKEIDKLDQSCDEEYANLVKNM 124
[Close]
BLAST of SMED30031990 vs. Planmine SMEST
Match: SMESG000025014.1 (SMESG000025014.1)

HSP 1 Score: 109.768 bits (273), Expect = 3.278e-28
Identity = 61/124 (49.19%), Postives = 81/124 (65.32%), Query Frame = 1 
Query:  343 MNKLLIFAFALLFVSIECRYKLVQ-ELSKRELSESCQKELDKMNEDYDKAVAKIDSDCDAAFLKIQEPFAPLWLDFESRCGLDFDKMSEECIDEAKKLDDDYTKEIKKLDSSCDAEFAKTVENL 711
            M   LI   A+LFVSIECRY+  + EL+KRELS  C KE++K++E  + A  KID+ C  A+  +    +  +L  E  CG   DKMS+EC  EAKKLDD ++KEI KLD SCD E+A  V+N+
Sbjct:    1 MKTFLIICLAILFVSIECRYRARRVELNKRELSAECSKEVEKLDEALNTATDKIDATCQKAYDDLVMKNSVEYLKIEKDCGNYIDKMSKECYAEAKKLDDSFSKEIDKLDQSCDEEYANLVKNM 124
[Close]
BLAST of SMED30031990 vs. Planmine SMEST
Match: SMESG000025004.1 (SMESG000025004.1)

HSP 1 Score: 109.768 bits (273), Expect = 5.213e-27
Identity = 61/124 (49.19%), Postives = 81/124 (65.32%), Query Frame = 1 
Query:  343 MNKLLIFAFALLFVSIECRYKLVQ-ELSKRELSESCQKELDKMNEDYDKAVAKIDSDCDAAFLKIQEPFAPLWLDFESRCGLDFDKMSEECIDEAKKLDDDYTKEIKKLDSSCDAEFAKTVENL 711
            M   LI   A+LFVSIECRY+  + EL+KRELS  C KE++K++E  + A  KID+ C  A+  +    +  +L  E  CG   DKMS+EC  EAKKLDD ++KEI KLD SCD E+A  V+N+
Sbjct:    1 MKTFLIICLAILFVSIECRYRARRVELNKRELSAECSKEVEKLDEALNTATDKIDASCQKAYDDLVMKNSVEYLKIEKDCGNYIDKMSKECYAEAKKLDDSFSKEIDKLDQSCDEEYANLVKNM 124

HSP 2 Score: 72.0182 bits (175), Expect = 5.353e-14
Identity = 38/102 (37.25%), Postives = 63/102 (61.76%), Query Frame = 1 
Query:  397 RYKLVQELSKRELSESCQKELDKMNEDYDKAVAKIDSDCDAAFLKIQEPFAPLWLDFESRCGLDFDKMSEECIDEAKKLDDDYTKEIKKLDSSCDAEFAKTV 702
            + KL+ EL K+ELS SCQ EL+ +  +++ A+ K+D DC+  + KI++ + P     +S C    DKMS++C DE     D +++ I  LD+SCD E+ + +
Sbjct:  128 KVKLLAELGKKELSRSCQNELNLIYSEFETAIEKVDEDCEDKYFKIKKEYKPDSGPVQSICTNIIDKMSKKCSDEMILEQDLFSEAIGNLDTSCDQEYERMI 229
[Close]
BLAST of SMED30031990 vs. Planmine SMEST
Match: SMESG000025002.1 (SMESG000025002.1)

HSP 1 Score: 104.375 bits (259), Expect = 3.481e-25
Identity = 55/124 (44.35%), Postives = 76/124 (61.29%), Query Frame = 1 
Query:  343 MNKLLIFAFALLFVSIECRYKLVQ-ELSKRELSESCQKELDKMNEDYDKAVAKIDSDCDAAFLKIQEPFAPLWLDFESRCGLDFDKMSEECIDEAKKLDDDYTKEIKKLDSSCDAEFAKTVENL 711
            M    I + A+LFVSIECRYK  + EL KRELS  C KE++K++   + A   ID  C   + +I   +   +L  E+ CG +   M++EC  E KKLD  ++KEI KLDSSCD E+A  V+N+
Sbjct:   99 MKAFFIISLAILFVSIECRYKARRVELFKRELSAECSKEVEKLDNQLNTAGDTIDKTCQKGYDEIMVKYGADFLKLETACGFEVSAMTKECSGEWKKLDSSFSKEIDKLDSSCDEEYANLVKNM 222
The following BLAST results are available for this feature:
BLAST of SMED30031990 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031990 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031990 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031990 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031990 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031990 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031990 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031990 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5N4B1W94.219e-833.13Uncharacterized protein OS=Photinus pyralis OX=705... [more]
K7UW318.403e-832.74General transcription factor 2-related zinc finger... [more]
A0A5N4B2948.999e-833.13Uncharacterized protein OS=Photinus pyralis OX=705... [more]
A0A1D6F7M22.424e-732.74General transcription factor 2-related zinc finger... [more]
A0A287PTU32.437e-732.74TTF-type domain-containing protein OS=Hordeum vulg... [more]
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BLAST of SMED30031990 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSAMXT00000037907.13.352e-647.83pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000044961.14.328e-646.67pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
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BLAST of SMED30031990 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031990 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031990 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031990 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSORLT00000028133.14.509e-751.22zinc finger MYM-type protein 1-like [Source:NCBI g... [more]
ENSORLT00000042294.14.509e-751.22zinc finger MYM-type protein 1-like [Source:NCBI g... [more]
ENSORLT00000045044.14.509e-751.22zinc finger MYM-type protein 1-like [Source:NCBI g... [more]
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BLAST of SMED30031990 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000025001.11.429e-7392.74SMESG000025001.1[more]
SMESG000054780.13.278e-2849.19SMESG000054780.1[more]
SMESG000025014.13.278e-2849.19SMESG000025014.1[more]
SMESG000025004.15.213e-2749.19SMESG000025004.1[more]
SMESG000025002.13.481e-2544.35SMESG000025002.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031990 ID=SMED30031990|Name=SMED30031990|organism=Schmidtea mediterranea sexual|type=transcript|length=1588bp
AAAACATAAGCATTGCTAATGCCTTTTTGCACAGAAATTGTGCATAAACC
GTATCAAAGTTGCCTCTTCATTTGCCATTCTTATTTCGGTTTGAAATTTA
TATGAATGTGCTCAGTTTTAGTCAAATTCCAGACATTGAAACACGAATGA
TACCTTACTAGATTGGACAGTTAAATAAAAAGTTAAGAATTAAAGGTTTT
CATGTGGTCAACCTATACTTAAGAATAAATTGAAATTTGTGCTACATTGG
ACTTGTTCATGGACCGTATCAAGCCTGTCAGTTTACTAGCAATTACCATA
TATATACCCTATGTATAACATTATCATTATCATTATTAAACCATGAATAA
ATTGTTAATTTTTGCATTTGCTCTCCTTTTTGTGTCCATAGAATGTAGGT
ACAAATTGGTACAAGAATTGTCGAAACGAGAATTGAGTGAATCGTGCCAA
AAAGAACTGGATAAAATGAATGAGGACTACGACAAGGCAGTAGCTAAAAT
AGATTCTGATTGTGATGCTGCATTTTTGAAAATCCAAGAACCATTTGCAC
CACTTTGGTTGGATTTCGAATCAAGATGTGGACTTGATTTTGATAAAATG
TCGGAAGAATGCATTGATGAAGCGAAGAAATTAGACGATGATTATACCAA
AGAAATCAAAAAACTCGACTCTTCTTGTGACGCGGAATTCGCAAAGACTG
TTGAGAACTTGAGTAAAGAAAAGGCAAAATTGAAGAAAGAATGTGCAGCA
CGTTAATAAAACTTATGCAAGTTTTAAATTTATTTTTCAAATATTTTGCG
TTGTTTTCATTTTTAACAAAGGTTATAACAATAATTGTTTGTTTTAACTT
GTTTCAACATTTCTCTTGAATTTGATATTTAATAATGTTGTAATATAATT
GTAATCATTCCCACAGATAAATGCAGAGTTCTAATTTTTGCAAGAATTTT
GAATCAAATTAAACAGTAAATATACAAACTCTAGGCATTGGACCTTTCAT
TTCAGCTTCCATTGTTTCTAGATTGTCAGTGATAAAATGGATACGATTTG
GGAAGTAGTTTATTTTGACAATTATTTTCAAGTAAATTATAGCCAAGGAC
CTAAGCAGCTGTAACTAAGGATCATTATACAATCTGCCAAATGAGTCAAT
TACTTAGAAAATAAATAATTGGATATGGAACTTTTCTAATAATTACAATA
GGTTTATATTAGTTTTTCAACCTTTTTTTCTTTAAAGAGATTGATTTTTG
TACGCGATTAATGTGGTTCGCCATTATATCATCAAACTTTCCTATCATTT
CAATTGATTTTAAAAAATTCCAATTATTTTTTTCATACAATTTGTTTGAA
CCGCCTCTTAAAGCTAAAACCCTGTCATGCAAGAAATTGTTCCACAGATA
TCAAACGTTCGACAATTCGGTAACATCGTGTTTTCTCGGCGTCATTGTAT
CGTTGCATATTTTTATCTATTGTACAGGTTTTTGTAAATTTAATTCAATA
TTTTTCTATTTTGTACAATTTTCAATATTCAGCAAAGTTTTTTCATGGCG
AGATAAGTTTTCAGAAGTGTATTGCCAATCATTGAAGC
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protein sequence of SMED30031990-orf-1

>SMED30031990-orf-1 ID=SMED30031990-orf-1|Name=SMED30031990-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=138bp
MNKLLIFAFALLFVSIECRYKLVQELSKRELSESCQKELDKMNEDYDKAV
AKIDSDCDAAFLKIQEPFAPLWLDFESRCGLDFDKMSEECIDEAKKLDDD
YTKEIKKLDSSCDAEFAKTVENLSKEKAKLKKECAAR*
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protein sequence of SMED30031990-orf-2

>SMED30031990-orf-2 ID=SMED30031990-orf-2|Name=SMED30031990-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=109bp
MWFAIISSNFPIISIDFKKFQLFFSYNLFEPPLKAKTLSCKKLFHRYQTF
DNSVTSCFLGVIVSLHIFIYCTGFCKFNSIFFYFVQFSIFSKVFSWRDKF
SEVYCQSLK
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Aliases
The feature 'SMED30031990' has the following synonyms
Synonym
prog2-5
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000028Category 2 cell
PLANA:0000101muscle cell
PLANA:0002032epidermal cell
PLANA:0002075pigment cell
PLANA:0002109X1 cell
PLANA:0002111X2 cell
PLANA:0003001lateral region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR023201SecY_dom_sf
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 113..137
NoneNo IPR availableCOILSCoilCoilcoord: 32..52
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..18
score: 0.74