Lysine-specific demethylase 5B

Overview
NameLysine-specific demethylase 5B
Smed IDSMED30031976
Length (bp)1182
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Lysine-specific demethylase 5B (SMED30031976) t-SNE clustered cells

Violin plots show distribution of expression levels for Lysine-specific demethylase 5B (SMED30031976) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Lysine-specific demethylase 5B (SMED30031976) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Lysine-specific demethylase 5B (SMED30031976) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30031976

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30031976 SmedASXL_006729SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Human
Match: KDM5A (lysine demethylase 5A [Source:HGNC Symbol;Acc:HGNC:9886])

HSP 1 Score: 56.9954 bits (136), Expect = 4.138e-8
Identity = 27/66 (40.91%), Postives = 38/66 (57.58%), Query Frame = 3 
Query:  747 SKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNCRSK 941
            S  E   DE  +CAA+ C RP    +DW+QCD  C  W+HQ+C+G++   A   ++ Y C NC  K
Sbjct: 1598 SGAEESDDENAVCAAQNCQRPCKDKVDWVQCDGGCDEWFHQVCVGVSPEMA--ENEDYICINCAKK 1661
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Human
Match: KDM5B (lysine demethylase 5B [Source:HGNC Symbol;Acc:HGNC:18039])

HSP 1 Score: 55.8398 bits (133), Expect = 9.863e-8
Identity = 26/69 (37.68%), Postives = 42/69 (60.87%), Query Frame = 3 
Query:  738 SSKSKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNCRSK 941
            +S S+ E  +DE+ +C A  C++P G  +DW+QCD  C+ W+HQ+C+G++   A    + Y C  C  K
Sbjct: 1467 TSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEMA--EKEDYICVRCTVK 1533
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Human
Match: KDM5B (lysine demethylase 5B [Source:HGNC Symbol;Acc:HGNC:18039])

HSP 1 Score: 55.8398 bits (133), Expect = 1.004e-7
Identity = 26/69 (37.68%), Postives = 42/69 (60.87%), Query Frame = 3 
Query:  738 SSKSKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNCRSK 941
            +S S+ E  +DE+ +C A  C++P G  +DW+QCD  C+ W+HQ+C+G++   A    + Y C  C  K
Sbjct: 1472 TSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEMA--EKEDYICVRCTVK 1538
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Human
Match: KDM5B (lysine demethylase 5B [Source:HGNC Symbol;Acc:HGNC:18039])

HSP 1 Score: 55.8398 bits (133), Expect = 1.054e-7
Identity = 26/69 (37.68%), Postives = 42/69 (60.87%), Query Frame = 3 
Query:  738 SSKSKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNCRSK 941
            +S S+ E  +DE+ +C A  C++P G  +DW+QCD  C+ W+HQ+C+G++   A    + Y C  C  K
Sbjct: 1431 TSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEMA--EKEDYICVRCTVK 1497
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Human
Match: KDM5B (lysine demethylase 5B [Source:HGNC Symbol;Acc:HGNC:18039])

HSP 1 Score: 55.8398 bits (133), Expect = 1.071e-7
Identity = 26/69 (37.68%), Postives = 42/69 (60.87%), Query Frame = 3 
Query:  738 SSKSKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNCRSK 941
            +S S+ E  +DE+ +C A  C++P G  +DW+QCD  C+ W+HQ+C+G++   A    + Y C  C  K
Sbjct: 1508 TSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEMA--EKEDYICVRCTVK 1574
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Celegans
Match: rbr-2 (Lysine-specific demethylase rbr-2 [Source:UniProtKB/Swiss-Prot;Acc:Q23541])

HSP 1 Score: 54.299 bits (129), Expect = 1.322e-7
Identity = 27/74 (36.49%), Postives = 43/74 (58.11%), Query Frame = 3 
Query:  723 KNTKRSSK-SKVELVQDEEEL--CAAEICMRPIGLAIDWIQCDI-CSSWYHQMCLGITTAKAIDPSKPYYCPNC 932
            K+ KR S+ S  E  +DE E   C A  C++P G +++W+ C+  C +W+H +CLG T  + I+    Y C +C
Sbjct: 1391 KSRKRQSQASPSEYYEDESEFKSCQARACLKPYGDSVNWVMCEAGCKNWFHVICLGFTL-REINDMHEYRCSSC 1463
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Celegans
Match: rbr-2 (Lysine-specific demethylase rbr-2 [Source:UniProtKB/Swiss-Prot;Acc:Q23541])

HSP 1 Score: 54.299 bits (129), Expect = 1.388e-7
Identity = 27/74 (36.49%), Postives = 43/74 (58.11%), Query Frame = 3 
Query:  723 KNTKRSSK-SKVELVQDEEEL--CAAEICMRPIGLAIDWIQCDI-CSSWYHQMCLGITTAKAIDPSKPYYCPNC 932
            K+ KR S+ S  E  +DE E   C A  C++P G +++W+ C+  C +W+H +CLG T  + I+    Y C +C
Sbjct: 1358 KSRKRQSQASPSEYYEDESEFKSCQARACLKPYGDSVNWVMCEAGCKNWFHVICLGFTL-REINDMHEYRCSSC 1430
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Fly
Match: lid (gene:FBgn0031759 transcript:FBtr0079231)

HSP 1 Score: 62.003 bits (149), Expect = 5.247e-10
Identity = 29/74 (39.19%), Postives = 43/74 (58.11%), Query Frame = 3 
Query:  714 GNIKNTKRSSKSKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNC 932
            G  K+ +RS ++  E   D+EE C AE C +P G  +DW+QCD  C+ W+H  C+G+  ++ I P   Y C  C
Sbjct: 1736 GQKKHAQRSQQAAQE---DDEEECRAENCHKPTGREVDWVQCDGGCNEWFHMYCVGLNRSQ-IKPDDDYICIRC 1805
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Fly
Match: lid (gene:FBgn0031759 transcript:FBtr0079232)

HSP 1 Score: 62.003 bits (149), Expect = 5.247e-10
Identity = 29/74 (39.19%), Postives = 43/74 (58.11%), Query Frame = 3 
Query:  714 GNIKNTKRSSKSKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNC 932
            G  K+ +RS ++  E   D+EE C AE C +P G  +DW+QCD  C+ W+H  C+G+  ++ I P   Y C  C
Sbjct: 1736 GQKKHAQRSQQAAQE---DDEEECRAENCHKPTGREVDWVQCDGGCNEWFHMYCVGLNRSQ-IKPDDDYICIRC 1805
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Fly
Match: lid (gene:FBgn0031759 transcript:FBtr0307070)

HSP 1 Score: 62.003 bits (149), Expect = 5.247e-10
Identity = 29/74 (39.19%), Postives = 43/74 (58.11%), Query Frame = 3 
Query:  714 GNIKNTKRSSKSKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNC 932
            G  K+ +RS ++  E   D+EE C AE C +P G  +DW+QCD  C+ W+H  C+G+  ++ I P   Y C  C
Sbjct: 1736 GQKKHAQRSQQAAQE---DDEEECRAENCHKPTGREVDWVQCDGGCNEWFHMYCVGLNRSQ-IKPDDDYICIRC 1805
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Fly
Match: lid (gene:FBgn0031759 transcript:FBtr0345336)

HSP 1 Score: 62.003 bits (149), Expect = 5.247e-10
Identity = 29/74 (39.19%), Postives = 43/74 (58.11%), Query Frame = 3 
Query:  714 GNIKNTKRSSKSKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNC 932
            G  K+ +RS ++  E   D+EE C AE C +P G  +DW+QCD  C+ W+H  C+G+  ++ I P   Y C  C
Sbjct: 1736 GQKKHAQRSQQAAQE---DDEEECRAENCHKPTGREVDWVQCDGGCNEWFHMYCVGLNRSQ-IKPDDDYICIRC 1805
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Fly
Match: lid (gene:FBgn0031759 transcript:FBtr0307071)

HSP 1 Score: 62.003 bits (149), Expect = 5.247e-10
Identity = 29/74 (39.19%), Postives = 43/74 (58.11%), Query Frame = 3 
Query:  714 GNIKNTKRSSKSKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNC 932
            G  K+ +RS ++  E   D+EE C AE C +P G  +DW+QCD  C+ W+H  C+G+  ++ I P   Y C  C
Sbjct: 1736 GQKKHAQRSQQAAQE---DDEEECRAENCHKPTGREVDWVQCDGGCNEWFHMYCVGLNRSQ-IKPDDDYICIRC 1805
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Zebrafish
Match: kdm5bb (lysine (K)-specific demethylase 5Bb [Source:NCBI gene;Acc:415256])

HSP 1 Score: 59.6918 bits (143), Expect = 3.588e-9
Identity = 32/91 (35.16%), Postives = 50/91 (54.95%), Query Frame = 3 
Query:  681 TKNSSSGKIRLGNIKNTKRS----SKSKVELVQDEEE---LCAAEICMRPIGLAIDWIQCDICSSWYHQMCLGITTAKAIDPSKPYYCPNC 932
            TK  S  +  +G  K  +R     S S +   +D EE   LC AE C++P G  +DW+QCD C+ W+H +C+G++   A +  + Y C +C
Sbjct: 1425 TKLKSKKQRMMGVEKRRERKAASVSASDMSQSEDSEEDMTLCPAESCLQPEGEEVDWVQCDCCNRWFHMICVGVSAELAAE--EDYMCVSC 1513
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Zebrafish
Match: kdm5bb (lysine (K)-specific demethylase 5Bb [Source:NCBI gene;Acc:415256])

HSP 1 Score: 59.6918 bits (143), Expect = 3.775e-9
Identity = 32/91 (35.16%), Postives = 50/91 (54.95%), Query Frame = 3 
Query:  681 TKNSSSGKIRLGNIKNTKRS----SKSKVELVQDEEE---LCAAEICMRPIGLAIDWIQCDICSSWYHQMCLGITTAKAIDPSKPYYCPNC 932
            TK  S  +  +G  K  +R     S S +   +D EE   LC AE C++P G  +DW+QCD C+ W+H +C+G++   A +  + Y C +C
Sbjct: 1406 TKLKSKKQRMMGVEKRRERKAASVSASDMSQSEDSEEDMTLCPAESCLQPEGEEVDWVQCDCCNRWFHMICVGVSAELAAE--EDYMCVSC 1494
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Zebrafish
Match: kdm5bb (lysine (K)-specific demethylase 5Bb [Source:NCBI gene;Acc:415256])

HSP 1 Score: 59.6918 bits (143), Expect = 3.787e-9
Identity = 32/91 (35.16%), Postives = 50/91 (54.95%), Query Frame = 3 
Query:  681 TKNSSSGKIRLGNIKNTKRS----SKSKVELVQDEEE---LCAAEICMRPIGLAIDWIQCDICSSWYHQMCLGITTAKAIDPSKPYYCPNC 932
            TK  S  +  +G  K  +R     S S +   +D EE   LC AE C++P G  +DW+QCD C+ W+H +C+G++   A +  + Y C +C
Sbjct: 1357 TKLKSKKQRMMGVEKRRERKAASVSASDMSQSEDSEEDMTLCPAESCLQPEGEEVDWVQCDCCNRWFHMICVGVSAELAAE--EDYMCVSC 1445
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Zebrafish
Match: kdm5ba (lysine (K)-specific demethylase 5Ba [Source:ZFIN;Acc:ZDB-GENE-030131-5379])

HSP 1 Score: 49.2914 bits (116), Expect = 8.363e-6
Identity = 19/57 (33.33%), Postives = 39/57 (68.42%), Query Frame = 3 
Query:  765 QDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNC 932
            +++  +C+A+ C +P G  ++W+QCD  C+ W+HQ+C+G++  +A   ++ Y C +C
Sbjct: 1414 EEDWSVCSAKRCQQPEGNEVNWVQCDGSCNQWFHQVCVGVSAEQA--ENEDYICVSC 1468
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Zebrafish
Match: kdm5ba (lysine (K)-specific demethylase 5Ba [Source:ZFIN;Acc:ZDB-GENE-030131-5379])

HSP 1 Score: 49.2914 bits (116), Expect = 8.365e-6
Identity = 19/57 (33.33%), Postives = 39/57 (68.42%), Query Frame = 3 
Query:  765 QDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNC 932
            +++  +C+A+ C +P G  ++W+QCD  C+ W+HQ+C+G++  +A   ++ Y C +C
Sbjct: 1417 EEDWSVCSAKRCQQPEGNEVNWVQCDGSCNQWFHQVCVGVSAEQA--ENEDYICVSC 1471
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Xenopus
Match: elp1 (elongator complex protein 1 [Source:Xenbase;Acc:XB-GENE-5813351])

HSP 1 Score: 58.5362 bits (140), Expect = 1.411e-8
Identity = 27/66 (40.91%), Postives = 39/66 (59.09%), Query Frame = 3 
Query:  747 SKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNCRSK 941
            S  E   DE  +CAA+ C RP    +DW+QCD  C  W+HQ+C+G+++  A   ++ Y C NC  K
Sbjct: 1507 SGAEESDDENAVCAAQNCQRPCKDKVDWVQCDGGCDEWFHQVCVGVSSEMA--ENEDYICTNCSRK 1570
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Xenopus
Match: elp1 (elongator complex protein 1 [Source:Xenbase;Acc:XB-GENE-5813351])

HSP 1 Score: 58.5362 bits (140), Expect = 1.475e-8
Identity = 27/66 (40.91%), Postives = 39/66 (59.09%), Query Frame = 3 
Query:  747 SKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNCRSK 941
            S  E   DE  +CAA+ C RP    +DW+QCD  C  W+HQ+C+G+++  A   ++ Y C NC  K
Sbjct: 1507 SGAEESDDENAVCAAQNCQRPCKDKVDWVQCDGGCDEWFHQVCVGVSSEMA--ENEDYICTNCSRK 1570
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Xenopus
Match: elp1 (elongator complex protein 1 [Source:Xenbase;Acc:XB-GENE-5813351])

HSP 1 Score: 58.151 bits (139), Expect = 1.579e-8
Identity = 27/66 (40.91%), Postives = 39/66 (59.09%), Query Frame = 3 
Query:  747 SKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNCRSK 941
            S  E   DE  +CAA+ C RP    +DW+QCD  C  W+HQ+C+G+++  A   ++ Y C NC  K
Sbjct: 1257 SGAEESDDENAVCAAQNCQRPCKDKVDWVQCDGGCDEWFHQVCVGVSSEMA--ENEDYICTNCSRK 1320
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Xenopus
Match: elp1 (elongator complex protein 1 [Source:Xenbase;Acc:XB-GENE-5813351])

HSP 1 Score: 58.151 bits (139), Expect = 1.648e-8
Identity = 27/66 (40.91%), Postives = 39/66 (59.09%), Query Frame = 3 
Query:  747 SKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNCRSK 941
            S  E   DE  +CAA+ C RP    +DW+QCD  C  W+HQ+C+G+++  A   ++ Y C NC  K
Sbjct: 1482 SGAEESDDENAVCAAQNCQRPCKDKVDWVQCDGGCDEWFHQVCVGVSSEMA--ENEDYICTNCSRK 1545
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Xenopus
Match: elp1 (elongator complex protein 1 [Source:Xenbase;Acc:XB-GENE-5813351])

HSP 1 Score: 57.7658 bits (138), Expect = 2.577e-8
Identity = 26/65 (40.00%), Postives = 39/65 (60.00%), Query Frame = 3 
Query:  747 SKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNCRS 938
            S  E   DE  +CAA+ C RP    +DW+QCD  C  W+HQ+C+G+++  A   ++ Y C NC +
Sbjct: 1255 SGAEESDDENAVCAAQNCQRPCKDKVDWVQCDGGCDEWFHQVCVGVSSEMA--ENEDYICTNCMA 1317
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Mouse
Match: Kdm5a (lysine (K)-specific demethylase 5A [Source:MGI Symbol;Acc:MGI:2136980])

HSP 1 Score: 57.7658 bits (138), Expect = 1.667e-8
Identity = 27/66 (40.91%), Postives = 38/66 (57.58%), Query Frame = 3 
Query:  747 SKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNCRSK 941
            S  E   DE  +CAA+ C RP    +DW+QCD  C  W+HQ+C+G++   A   ++ Y C NC  K
Sbjct: 1598 SGAEESDDENAVCAAQNCQRPCKDKVDWVQCDGGCDEWFHQVCVGVSAEMA--ENEDYICINCAKK 1661
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Mouse
Match: Kdm5b (lysine (K)-specific demethylase 5B [Source:MGI Symbol;Acc:MGI:1922855])

HSP 1 Score: 56.6102 bits (135), Expect = 4.341e-8
Identity = 26/69 (37.68%), Postives = 42/69 (60.87%), Query Frame = 3 
Query:  738 SSKSKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNCRSK 941
            +S S+ E  +DE+ +C A  C++P G  +DW+QCD  C+ W+HQ+C+G++   A    + Y C  C  K
Sbjct: 1472 TSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEMA--EKEDYICVRCTGK 1538
[Close]
BLAST of Lysine-specific demethylase 5B vs. UniProt/SwissProt
Match: sp|Q9VMJ7|KDM5_DROME (Lysine-specific demethylase lid OS=Drosophila melanogaster OX=7227 GN=lid PE=1 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 5.260e-9
Identity = 29/74 (39.19%), Postives = 43/74 (58.11%), Query Frame = 3 
Query:  714 GNIKNTKRSSKSKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNC 932
            G  K+ +RS ++  E   D+EE C AE C +P G  +DW+QCD  C+ W+H  C+G+  ++ I P   Y C  C
Sbjct: 1736 GQKKHAQRSQQAAQE---DDEEECRAENCHKPTGREVDWVQCDGGCNEWFHMYCVGLNRSQ-IKPDDDYICIRC 1805
[Close]
BLAST of Lysine-specific demethylase 5B vs. UniProt/SwissProt
Match: sp|Q6IQX0|KD5BB_DANRE (Lysine-specific demethylase 5B-B OS=Danio rerio OX=7955 GN=kdm5bb PE=2 SV=2)

HSP 1 Score: 59.6918 bits (143), Expect = 2.824e-8
Identity = 32/91 (35.16%), Postives = 50/91 (54.95%), Query Frame = 3 
Query:  681 TKNSSSGKIRLGNIKNTKRS----SKSKVELVQDEEE---LCAAEICMRPIGLAIDWIQCDICSSWYHQMCLGITTAKAIDPSKPYYCPNC 932
            TK  S  +  +G  K  +R     S S +   +D EE   LC AE C++P G  +DW+QCD C+ W+H +C+G++   A +  + Y C +C
Sbjct: 1406 TKLKSKKQRMMGVEKRRERKAASVSASDMSQSEDSEEDMTLCPAESCLQPEGEEVDWVQCDCCNRWFHMICVGVSAELAAE--EDYMCVSC 1494
[Close]
BLAST of Lysine-specific demethylase 5B vs. UniProt/SwissProt
Match: sp|Q5F3R2|KDM5B_CHICK (Lysine-specific demethylase 5B OS=Gallus gallus OX=9031 GN=KDM5B PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 6.391e-8
Identity = 27/69 (39.13%), Postives = 43/69 (62.32%), Query Frame = 3 
Query:  738 SSKSKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNCRSK 941
            +S S+ E  +DE+ +C A  C++P G  +DW+QCD  C+ W+HQ+C+GI+   A    + Y C +C  K
Sbjct: 1450 TSFSEQEDSEDEDAICPAVTCLQPEGEEVDWVQCDGSCNQWFHQVCVGISPEMA--EKEDYICASCAGK 1516
[Close]
BLAST of Lysine-specific demethylase 5B vs. UniProt/SwissProt
Match: sp|Q3UXZ9|KDM5A_MOUSE (Lysine-specific demethylase 5A OS=Mus musculus OX=10090 GN=Kdm5a PE=1 SV=2)

HSP 1 Score: 57.7658 bits (138), Expect = 1.166e-7
Identity = 27/66 (40.91%), Postives = 38/66 (57.58%), Query Frame = 3 
Query:  747 SKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNCRSK 941
            S  E   DE  +CAA+ C RP    +DW+QCD  C  W+HQ+C+G++   A   ++ Y C NC  K
Sbjct: 1598 SGAEESDDENAVCAAQNCQRPCKDKVDWVQCDGGCDEWFHQVCVGVSAEMA--ENEDYICINCAKK 1661
[Close]
BLAST of Lysine-specific demethylase 5B vs. UniProt/SwissProt
Match: sp|P29375|KDM5A_HUMAN (Lysine-specific demethylase 5A OS=Homo sapiens OX=9606 GN=KDM5A PE=1 SV=3)

HSP 1 Score: 56.9954 bits (136), Expect = 1.987e-7
Identity = 27/66 (40.91%), Postives = 38/66 (57.58%), Query Frame = 3 
Query:  747 SKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNCRSK 941
            S  E   DE  +CAA+ C RP    +DW+QCD  C  W+HQ+C+G++   A   ++ Y C NC  K
Sbjct: 1598 SGAEESDDENAVCAAQNCQRPCKDKVDWVQCDGGCDEWFHQVCVGVSPEMA--ENEDYICINCAKK 1661
[Close]
BLAST of Lysine-specific demethylase 5B vs. TrEMBL
Match: A0A2H1C0L0 (FHA domain protein OS=Fasciola hepatica OX=6192 GN=D915_12156 PE=4 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 3.310e-28
Identity = 97/268 (36.19%), Postives = 133/268 (49.63%), Query Frame = 3 
Query:  129 MTTCRLTLIGSLSENQ-------NSCDTPNLPKHIIIHDEPDEIVTIGRENSKTKVNYVLGDSSKPRVVSRLHATLKYNADDLSFWLSDQSVNGTYVNYVRVPVSGLKLEMEDIVCFFHRNGANIKPGEKVEPFDTHLKYRVXXXXXXXXXXXXXXAQS-KSSKEPLK------TSKKRGSFILDRSCSPETIAKKPRTK----NSSSGKIR----LGNIKNTKRSSKSKVELVQDEEELCAAEICMRPIGLAIDWIQCDICSSWYHQ 866
            M+T  L  IG L +++       +S      PK I +     EI  IGR N+K   +Y +      R++SR HAT++ +A   S  L D S+NGTYVNY RV + G+ L+  DIVCF H NGAN+KPGEKV  F + LKY+VE  D            S K  K  +       +S +R     D S      AK+ ++     N S GK +      +   T  +  S  E+   + E C+A  C +P  +AIDWIQCD C+ WYHQ
Sbjct:    1 MSTVTLRWIGDLDQDKREQAGCVSSSSDQYRPKKIELKPGQKEI-RIGRINTKMPPDYPIESCINSRMISRNHATIERSAKGGSM-LYDHSMNGTYVNYTRV-IGGVTLKHGDIVCFGHLNGANLKPGEKVSLFYSDLKYQVELSDAPPSKETPANTGSVKKRKSHMAHSGSTVSSTQRSEEDDDASGDSGNDAKRSKSSAPSTNKSHGKEKRTKSSKSKSGTDDADASGNEMFHYDTEECSARPCKQPQDIAIDWIQCDKCTLWYHQ 265
[Close]
BLAST of Lysine-specific demethylase 5B vs. TrEMBL
Match: A0A0R3U9D6 (Uncharacterized protein (Fragment) OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS3535 PE=4 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 6.579e-20
Identity = 65/174 (37.36%), Postives = 93/174 (53.45%), Query Frame = 3 
Query:  129 MTTCRLTLIGSLSENQNSC-------DTPNLPKHIIIHDEPDEIVTIGRENSKTKVNYVLGDSSKPRVVSRLHATLKYNADDLSFWLSDQSVNGTYVNYVRVPVSGLKLEMEDIVCFFHRNGANIKPGEKVEPFDTHLKYRVXXXXXXXXXXXXXXAQSKSSKEPLKTSKKRGS 629
            M+T  L  +G L++++          D    PK I + +   EI  IGR NS    +Y++      R++SR HAT++  A+   F L D S+NGTYVNY+RV   G+ L+  DIVCF H NGAN+KPGE V PF + LKY+V        +     A   S + P    ++RG+
Sbjct:    1 MSTVSLLWVGDLNQDKRMANASMKISDERYRPKLIELKEGQKEI-RIGRLNSTNPPHYLIESCINKRMISRNHATIERLANG-GFMLYDHSMNGTYVNYMRVS-GGVTLKHGDIVCFGHLNGANLKPGEDVTPFFSDLKYQV--YIGSTRDAINSAANDASGERPSGNKRQRGA 169
[Close]
BLAST of Lysine-specific demethylase 5B vs. TrEMBL
Match: A0A5K3FSW1 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 8.068e-20
Identity = 65/174 (37.36%), Postives = 93/174 (53.45%), Query Frame = 3 
Query:  129 MTTCRLTLIGSLSENQNSC-------DTPNLPKHIIIHDEPDEIVTIGRENSKTKVNYVLGDSSKPRVVSRLHATLKYNADDLSFWLSDQSVNGTYVNYVRVPVSGLKLEMEDIVCFFHRNGANIKPGEKVEPFDTHLKYRVXXXXXXXXXXXXXXAQSKSSKEPLKTSKKRGS 629
            M+T  L  +G L++++          D    PK I + +   EI  IGR NS    +Y++      R++SR HAT++  A+   F L D S+NGTYVNY+RV   G+ L+  DIVCF H NGAN+KPGE V PF + LKY+V        +     A   S + P    ++RG+
Sbjct:    1 MSTVSLLWVGDLNQDKRMANASMKISDERYRPKLIELKEGQKEI-RIGRLNSTNPPHYLIESCINKRMISRNHATIERLANG-GFMLYDHSMNGTYVNYMRVS-GGVTLKHGDIVCFGHLNGANLKPGEDVTPFFSDLKYQV--YIGSTRDAINSAANDASGERPSGNKRQRGA 169
[Close]
BLAST of Lysine-specific demethylase 5B vs. TrEMBL
Match: U6J9T7 (Lysine-specific demethylase 5B OS=Echinococcus granulosus OX=6210 GN=EGR_07596 PE=4 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.894e-19
Identity = 61/142 (42.96%), Postives = 83/142 (58.45%), Query Frame = 3 
Query:  129 MTTCRLTLIGSLSENQNSCDTP-------NLPKHIIIHDEPDEIVTIGRENSKTKVNYVLGDSSKPRVVSRLHATLKYNADDLSFWLSDQSVNGTYVNYVRVPVSGLKLEMEDIVCFFHRNGANIKPGEKVEPFDTHLKYRV 533
            MTT  L  +G LS+++    T          PK I + ++  EI  IGR NS    +Y++      R++SR HAT++    D  F L D S+NGTYVNY+RV   G+ L+  DIVCF H NGAN+KPG+ V PF + LKY+V
Sbjct:    1 MTTVSLLWVGDLSQDKRMASTSMKVSDERYRPKLIELKEDQKEI-RIGRLNSTNPPHYLIESCINKRMISRNHATIE-RLPDGGFMLYDHSMNGTYVNYIRVS-GGVTLKSGDIVCFGHLNGANLKPGDDVTPFFSDLKYQV 139

HSP 2 Score: 77.0258 bits (188), Expect = 7.766e-12
Identity = 32/60 (53.33%), Postives = 42/60 (70.00%), Query Frame = 3 
Query:  765 QDEEELCAAEICMRPIGLAIDWIQCDICSSWYHQMCLGITTAKA-IDPSKPYYCPNCRSK 941
            +D++E C+A  C +P G AIDWIQCD CS WYHQ+CL +T  +A +D    YYCP+C  K
Sbjct:  298 EDDQEECSARTCKKPAGTAIDWIQCDKCSKWYHQICLNLTPEEADVD---VYYCPSCTKK 354
[Close]
BLAST of Lysine-specific demethylase 5B vs. TrEMBL
Match: A0A068Y6Q8 (Lysine specific demethylase 5B OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000516400 PE=4 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 2.393e-19
Identity = 61/142 (42.96%), Postives = 83/142 (58.45%), Query Frame = 3 
Query:  129 MTTCRLTLIGSLSENQNSCDTP-------NLPKHIIIHDEPDEIVTIGRENSKTKVNYVLGDSSKPRVVSRLHATLKYNADDLSFWLSDQSVNGTYVNYVRVPVSGLKLEMEDIVCFFHRNGANIKPGEKVEPFDTHLKYRV 533
            MTT  L  +G LS+++    T          PK I + ++  EI  IGR NS    +Y++      R++SR HAT++    D  F L D S+NGTYVNY+RV   G+ L+  DIVCF H NGAN+KPG+ V PF + LKY+V
Sbjct:    1 MTTVSLLWVGDLSQDKRMASTSMKVSDERYRPKLIELKEDQKEI-RIGRLNSTNPPHYLIESCINKRMISRNHATIE-RLPDGGFMLYDHSMNGTYVNYIRVS-GGVTLKSGDIVCFGHLNGANLKPGDDVTPFFSDLKYQV 139

HSP 2 Score: 77.0258 bits (188), Expect = 9.094e-12
Identity = 32/60 (53.33%), Postives = 42/60 (70.00%), Query Frame = 3 
Query:  765 QDEEELCAAEICMRPIGLAIDWIQCDICSSWYHQMCLGITTAKA-IDPSKPYYCPNCRSK 941
            +D++E C+A  C +P G AIDWIQCD CS WYHQ+CL +T  +A +D    YYCP+C  K
Sbjct:  298 EDDQEECSARTCKKPAGTAIDWIQCDKCSKWYHQICLNLTPEEADVD---VYYCPSCTKK 354
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Cavefish
Match: kdm5bb (lysine-specific demethylase 5B-B-like [Source:NCBI gene;Acc:103026437])

HSP 1 Score: 56.225 bits (134), Expect = 4.797e-8
Identity = 24/61 (39.34%), Postives = 37/61 (60.66%), Query Frame = 3 
Query:  768 DEEE---LCAAEICMRPIGLAIDWIQCDICSSWYHQMCLGITTAKAIDPSKPYYCPNCRSK 941
            D EE   LC AE C +P G  + W+QCD C+ W+H +C+G++   A +  + Y C +C S+
Sbjct: 1404 DSEEDMILCPAESCRQPAGDEVYWVQCDCCNQWFHMVCVGVSAKMAAE--EDYVCVSCSSR 1462
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Cavefish
Match: kdm5bb (lysine-specific demethylase 5B-B-like [Source:NCBI gene;Acc:103026437])

HSP 1 Score: 55.8398 bits (133), Expect = 5.457e-8
Identity = 24/61 (39.34%), Postives = 37/61 (60.66%), Query Frame = 3 
Query:  768 DEEE---LCAAEICMRPIGLAIDWIQCDICSSWYHQMCLGITTAKAIDPSKPYYCPNCRSK 941
            D EE   LC AE C +P G  + W+QCD C+ W+H +C+G++   A +  + Y C +C S+
Sbjct: 1470 DSEEDMILCPAESCRQPAGDEVYWVQCDCCNQWFHMVCVGVSAKMAAE--EDYVCVSCSSR 1528
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Cavefish
Match: kdm5bb (lysine-specific demethylase 5B-B-like [Source:NCBI gene;Acc:103026437])

HSP 1 Score: 55.8398 bits (133), Expect = 5.718e-8
Identity = 24/61 (39.34%), Postives = 37/61 (60.66%), Query Frame = 3 
Query:  768 DEEE---LCAAEICMRPIGLAIDWIQCDICSSWYHQMCLGITTAKAIDPSKPYYCPNCRSK 941
            D EE   LC AE C +P G  + W+QCD C+ W+H +C+G++   A +  + Y C +C S+
Sbjct: 1414 DSEEDMILCPAESCRQPAGDEVYWVQCDCCNQWFHMVCVGVSAKMAAE--EDYVCVSCSSR 1472
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Cavefish
Match: kdm5bb (lysine-specific demethylase 5B-B-like [Source:NCBI gene;Acc:103026437])

HSP 1 Score: 55.8398 bits (133), Expect = 5.739e-8
Identity = 24/61 (39.34%), Postives = 37/61 (60.66%), Query Frame = 3 
Query:  768 DEEE---LCAAEICMRPIGLAIDWIQCDICSSWYHQMCLGITTAKAIDPSKPYYCPNCRSK 941
            D EE   LC AE C +P G  + W+QCD C+ W+H +C+G++   A +  + Y C +C S+
Sbjct: 1451 DSEEDMILCPAESCRQPAGDEVYWVQCDCCNQWFHMVCVGVSAKMAAE--EDYVCVSCSSR 1509
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Cavefish
Match: kdm5ba (lysine (K)-specific demethylase 5Ba [Source:ZFIN;Acc:ZDB-GENE-030131-5379])

HSP 1 Score: 53.5286 bits (127), Expect = 3.078e-7
Identity = 21/60 (35.00%), Postives = 41/60 (68.33%), Query Frame = 3 
Query:  765 QDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNCRSK 941
            +++  +C+A+ C++P G  ++W+QCD  C+ W+HQ+C+G++  +A   ++ Y C NC  K
Sbjct: 1342 EEDWSVCSAKRCLQPEGNEVNWVQCDGSCNQWFHQVCVGVSAEQA--ENEDYICVNCSRK 1399
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Nematostella
Match: EDO34883 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SN37])

HSP 1 Score: 57.7658 bits (138), Expect = 4.740e-9
Identity = 24/55 (43.64%), Postives = 35/55 (63.64%), Query Frame = 3 
Query:  783 CAAEICMRPIGLAIDWIQCDICSSWYHQMCLGITT--AKAIDPSKPYYCPNCRSK 941
            CAA  C RPIG  + W+QCD C  WYH +C+G+++  A+A+D    Y+C  C  +
Sbjct: 1003 CAAASCSRPIGEEVGWVQCDQCERWYHLVCIGLSSERAEALD---SYHCKLCTGQ 1054
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Nematostella
Match: EDO47828 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RLA5])

HSP 1 Score: 51.6026 bits (122), Expect = 2.161e-7
Identity = 32/99 (32.32%), Postives = 60/99 (60.61%), Query Frame = 3 
Query:  246 IGRENSKTKVNYVLGDSSKPR-VVSRLHATLKYNADDLSFWLS--DQSVNGTYVNYVRVPVSGLKLEMEDIVCFFHRNGANIKPGEKVEPFDTHLKYRV 533
            +GR   K+ V Y+  DS K + ++SRLHA + Y  D+ +  L+  D S+NGT+VN  ++ +  +++++ D+V F H  GA + PG   +  D+  ++++
Sbjct:   34 VGRNPGKSDV-YL--DSMKHKALISRLHARIIYTTDNDTAKLTICDTSLNGTFVNDRKI-IDTVEVKLGDVVTFGHLRGAVLAPGTVAKQPDSEFRFKL 128
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Nematostella
Match: EDO39503 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S9X9])

HSP 1 Score: 47.3654 bits (111), Expect = 6.384e-6
Identity = 26/74 (35.14%), Postives = 35/74 (47.30%), Query Frame = 3 
Query:  759 LVQDEEELCAAEICMRPIGLAIDWIQCDICSSWYHQMCLGITTAKAIDPSKPYYCPNC---RSK*NFKNSRNWQ 971
            +   +E+ C   IC RP       IQCD C  W+H  C+GI   +A D  + Y+CP+C         K  RNW 
Sbjct:    1 MADHQEQYC---ICRRPYEPEEFMIQCDSCQDWFHGSCVGIEEYQASDIER-YHCPSCAELYGPLTLKKRRNWH 70
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Medaka
Match: kdm5a (lysine demethylase 5A [Source:NCBI gene;Acc:101161451])

HSP 1 Score: 55.4546 bits (132), Expect = 8.310e-8
Identity = 24/59 (40.68%), Postives = 36/59 (61.02%), Query Frame = 3 
Query:  768 DEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNCRSK 941
            DE  +CAA+ C RP    +DW+QCD  C  W+HQ+C+G++   A   ++ Y C +C  K
Sbjct: 1624 DENAVCAAKNCQRPCKDKVDWVQCDGGCDEWFHQVCVGVSCEMA--ETEDYICMDCSQK 1680
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Medaka
Match: kdm5a (lysine demethylase 5A [Source:NCBI gene;Acc:101161451])

HSP 1 Score: 55.0694 bits (131), Expect = 1.029e-7
Identity = 26/66 (39.39%), Postives = 38/66 (57.58%), Query Frame = 3 
Query:  747 SKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNCRSK 941
            S  E   DE  +CAA+ C RP    +DW+QCD  C  W+HQ+C+G++   A   ++ Y C +C  K
Sbjct: 1497 SGAEDSNDENAVCAAKNCQRPCKDKVDWVQCDGGCDEWFHQVCVGVSCEMA--ETEDYICMDCSQK 1560
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Medaka
Match: kdm5bb (lysine-specific demethylase 5B-B [Source:NCBI gene;Acc:101155110])

HSP 1 Score: 52.7582 bits (125), Expect = 5.657e-7
Identity = 26/66 (39.39%), Postives = 37/66 (56.06%), Query Frame = 3 
Query:  747 SKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNCRSK 941
            S+ E   +E  +C AE C  P G  +DW+QCD  C+ W+HQ+C+G+T   A    + Y C  C  K
Sbjct: 1477 SQSEDSDEEMAVCPAEDCQLPEGDEVDWVQCDGSCNQWFHQVCVGVTPEMA--EKEDYVCVTCTLK 1540
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Medaka
Match: kdm5bb (lysine-specific demethylase 5B-B [Source:NCBI gene;Acc:101155110])

HSP 1 Score: 52.7582 bits (125), Expect = 5.950e-7
Identity = 26/66 (39.39%), Postives = 37/66 (56.06%), Query Frame = 3 
Query:  747 SKVELVQDEEELCAAEICMRPIGLAIDWIQCD-ICSSWYHQMCLGITTAKAIDPSKPYYCPNCRSK 941
            S+ E   +E  +C AE C  P G  +DW+QCD  C+ W+HQ+C+G+T   A    + Y C  C  K
Sbjct: 1458 SQSEDSDEEMAVCPAEDCQLPEGDEVDWVQCDGSCNQWFHQVCVGVTPEMA--EKEDYVCVTCTLK 1521
[Close]
BLAST of Lysine-specific demethylase 5B vs. Ensembl Medaka
Match: tcf19l (transcription factor 19 [Source:NCBI gene;Acc:105355154])

HSP 1 Score: 51.9878 bits (123), Expect = 6.407e-7
Identity = 23/54 (42.59%), Postives = 31/54 (57.41%), Query Frame = 3 
Query:  777 ELCAAEICMRPIGLAIDWIQCDICSSWYHQMCLGITTAKAI-DPSKPYYCPNCR 935
            E CAA  C  P    + WIQCD+C +WYH  CL +   K + DP+  ++C  CR
Sbjct:  513 EPCAAPRCRLPQQDTVQWIQCDVCDAWYHLDCLHVDRKKLLKDPNADFHC-GCR 565
[Close]
BLAST of Lysine-specific demethylase 5B vs. Planmine SMEST
Match: SMESG000051866.1 (SMESG000051866.1)

HSP 1 Score: 100.908 bits (250), Expect = 2.921e-23
Identity = 55/111 (49.55%), Postives = 72/111 (64.86%), Query Frame = 3 
Query:  201 PKHIIIHDEPDEIVTIGRENSKTKVNYVLGDSSKPRVVSRLHATLKYNADDLSFWLSDQSVNGTYVNYVRVPVSGLKLEMEDIVCFFHRNGANIKPGEKVEPFDTHLKYRV 533
            PK +I+  +  E   IGR+NSK   +Y L  +   R++SR H T++      ++ L D S+NGTYVN+VRV + G  L+ ED+VCF H NGANIKPGE V PF T LKYRV
Sbjct:   33 PKKMIVRQDKKE-TRIGRKNSKMPPDYALESAVSNRMISRNHVTIE-RLSKGNYMLYDHSLNGTYVNFVRV-IGGTILKHEDVVCFGHINGANIKPGESVTPFATDLKYRV 140

HSP 2 Score: 65.0846 bits (157), Expect = 2.522e-11
Identity = 43/124 (34.68%), Postives = 65/124 (52.42%), Query Frame = 3 
Query:  579 QSKSSKEPLKTSKKR---GSFILDRSCS-PETIAKKPRTKNSSSGKIRLGNIKNTKRSSKSKVELVQDEE-ELCAAEICMRPIG-LAIDWIQCDICSSWYHQMCLGITTAKAIDPSKPYYCPNC 932
            +S  SK P+K  + R    S + DR     +    KP+  +++      GN  N  +S K  V L  + + E C+A  C+RP G   IDWI CD C+ W+HQ C+G+   +A++  + YYCP C
Sbjct:  295 KSHESKRPMKGDEDRRMLSSAVKDRKLHLNQKSHSKPKYSDNN------GNT-NANKSMKRNVNLNSNYDYEECSARPCLRPDGETLIDWIMCDKCTQWFHQSCVGLD--EAVEDDESYYCPYC 409
The following BLAST results are available for this feature:
BLAST of Lysine-specific demethylase 5B vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
KDM5A4.138e-840.91lysine demethylase 5A [Source:HGNC Symbol;Acc:HGNC... [more]
KDM5B9.863e-837.68lysine demethylase 5B [Source:HGNC Symbol;Acc:HGNC... [more]
KDM5B1.004e-737.68lysine demethylase 5B [Source:HGNC Symbol;Acc:HGNC... [more]
KDM5B1.054e-737.68lysine demethylase 5B [Source:HGNC Symbol;Acc:HGNC... [more]
KDM5B1.071e-737.68lysine demethylase 5B [Source:HGNC Symbol;Acc:HGNC... [more]
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BLAST of Lysine-specific demethylase 5B vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rbr-21.322e-736.49Lysine-specific demethylase rbr-2 [Source:UniProt... [more]
rbr-21.388e-736.49Lysine-specific demethylase rbr-2 [Source:UniProt... [more]
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BLAST of Lysine-specific demethylase 5B vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
lid5.247e-1039.19gene:FBgn0031759 transcript:FBtr0079231[more]
lid5.247e-1039.19gene:FBgn0031759 transcript:FBtr0079232[more]
lid5.247e-1039.19gene:FBgn0031759 transcript:FBtr0307070[more]
lid5.247e-1039.19gene:FBgn0031759 transcript:FBtr0345336[more]
lid5.247e-1039.19gene:FBgn0031759 transcript:FBtr0307071[more]
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BLAST of Lysine-specific demethylase 5B vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
kdm5bb3.588e-935.16lysine (K)-specific demethylase 5Bb [Source:NCBI g... [more]
kdm5bb3.775e-935.16lysine (K)-specific demethylase 5Bb [Source:NCBI g... [more]
kdm5bb3.787e-935.16lysine (K)-specific demethylase 5Bb [Source:NCBI g... [more]
kdm5ba8.363e-633.33lysine (K)-specific demethylase 5Ba [Source:ZFIN;A... [more]
kdm5ba8.365e-633.33lysine (K)-specific demethylase 5Ba [Source:ZFIN;A... [more]
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BLAST of Lysine-specific demethylase 5B vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
elp11.411e-840.91elongator complex protein 1 [Source:Xenbase;Acc:XB... [more]
elp11.475e-840.91elongator complex protein 1 [Source:Xenbase;Acc:XB... [more]
elp11.579e-840.91elongator complex protein 1 [Source:Xenbase;Acc:XB... [more]
elp11.648e-840.91elongator complex protein 1 [Source:Xenbase;Acc:XB... [more]
elp12.577e-840.00elongator complex protein 1 [Source:Xenbase;Acc:XB... [more]
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BLAST of Lysine-specific demethylase 5B vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Kdm5a1.667e-840.91lysine (K)-specific demethylase 5A [Source:MGI Sym... [more]
Kdm5b4.341e-837.68lysine (K)-specific demethylase 5B [Source:MGI Sym... [more]
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BLAST of Lysine-specific demethylase 5B vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9VMJ7|KDM5_DROME5.260e-939.19Lysine-specific demethylase lid OS=Drosophila mela... [more]
sp|Q6IQX0|KD5BB_DANRE2.824e-835.16Lysine-specific demethylase 5B-B OS=Danio rerio OX... [more]
sp|Q5F3R2|KDM5B_CHICK6.391e-839.13Lysine-specific demethylase 5B OS=Gallus gallus OX... [more]
sp|Q3UXZ9|KDM5A_MOUSE1.166e-740.91Lysine-specific demethylase 5A OS=Mus musculus OX=... [more]
sp|P29375|KDM5A_HUMAN1.987e-740.91Lysine-specific demethylase 5A OS=Homo sapiens OX=... [more]
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BLAST of Lysine-specific demethylase 5B vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2H1C0L03.310e-2836.19FHA domain protein OS=Fasciola hepatica OX=6192 GN... [more]
A0A0R3U9D66.579e-2037.36Uncharacterized protein (Fragment) OS=Mesocestoide... [more]
A0A5K3FSW18.068e-2037.36Uncharacterized protein OS=Mesocestoides corti OX=... [more]
U6J9T71.894e-1942.96Lysine-specific demethylase 5B OS=Echinococcus gra... [more]
A0A068Y6Q82.393e-1942.96Lysine specific demethylase 5B OS=Echinococcus mul... [more]
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BLAST of Lysine-specific demethylase 5B vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
kdm5bb4.797e-839.34lysine-specific demethylase 5B-B-like [Source:NCBI... [more]
kdm5bb5.457e-839.34lysine-specific demethylase 5B-B-like [Source:NCBI... [more]
kdm5bb5.718e-839.34lysine-specific demethylase 5B-B-like [Source:NCBI... [more]
kdm5bb5.739e-839.34lysine-specific demethylase 5B-B-like [Source:NCBI... [more]
kdm5ba3.078e-735.00lysine (K)-specific demethylase 5Ba [Source:ZFIN;A... [more]
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BLAST of Lysine-specific demethylase 5B vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Lysine-specific demethylase 5B vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Lysine-specific demethylase 5B vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3
Match NameE-valueIdentityDescription
EDO348834.740e-943.64Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO478282.161e-732.32Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO395036.384e-635.14Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Lysine-specific demethylase 5B vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
kdm5a8.310e-840.68lysine demethylase 5A [Source:NCBI gene;Acc:101161... [more]
kdm5a1.029e-739.39lysine demethylase 5A [Source:NCBI gene;Acc:101161... [more]
kdm5bb5.657e-739.39lysine-specific demethylase 5B-B [Source:NCBI gene... [more]
kdm5bb5.950e-739.39lysine-specific demethylase 5B-B [Source:NCBI gene... [more]
tcf19l6.407e-742.59transcription factor 19 [Source:NCBI gene;Acc:1053... [more]
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BLAST of Lysine-specific demethylase 5B vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000051866.12.921e-2349.55SMESG000051866.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031976 ID=SMED30031976|Name=Lysine-specific demethylase 5B|organism=Schmidtea mediterranea sexual|type=transcript|length=1182bp
CCATATTTATGGATTTGTTTTAATATACTTTAAGAATTTTGTAATTAAAT
AAGAATCTATTATAATTGTATCTAGTTTCTGAGTTTTAAAATATTTAGTT
TTATAAGATATATATATCAGACATAATTATGACCACGTGTAGATTAACAT
TAATTGGTAGTTTATCGGAAAATCAGAATTCATGTGACACTCCAAATTTA
CCAAAACATATTATAATTCATGATGAACCTGACGAAATTGTTACAATTGG
TCGAGAAAATTCAAAAACGAAAGTTAATTATGTTCTCGGTGATTCATCTA
AACCTCGAGTTGTTAGTCGATTACATGCTACATTAAAATATAACGCTGAT
GATCTCTCGTTTTGGTTATCTGATCAAAGTGTTAATGGAACATATGTTAA
TTATGTTCGAGTTCCAGTAAGTGGCCTTAAACTTGAAATGGAAGATATCG
TGTGCTTTTTTCATCGAAATGGAGCTAATATCAAACCTGGTGAAAAGGTA
GAACCATTTGATACTCATTTAAAATACCGAGTGGAATTTGTTGATGAAGA
TGATGAAAATTTGGATGAAGATGAGGCTCAGTCAAAATCTTCTAAAGAAC
CTCTGAAAACTTCTAAAAAACGTGGTAGTTTTATTCTCGATAGGTCTTGC
AGTCCAGAGACAATAGCTAAAAAACCTCGTACGAAAAACAGTTCTAGTGG
TAAAATAAGATTAGGAAACATTAAAAATACTAAACGTTCGTCAAAATCGA
AAGTAGAATTAGTTCAAGACGAAGAAGAGTTATGTGCAGCTGAAATATGC
ATGAGACCAATTGGACTAGCTATAGATTGGATACAGTGTGATATTTGTTC
CAGTTGGTATCATCAAATGTGTTTAGGAATCACTACAGCTAAAGCTATAG
ATCCCTCAAAACCCTATTATTGTCCAAATTGTCGAAGCAAATAAAATTTT
AAAAATTCTCGAAATTGGCAGCTGACCATTTTATCAAAAAATTATTACAA
TTAAACTTACTCAACTGCAAAACTTGTTTCAATTGATGTATAAAGAAAAG
CGGGATTTTTGTAAATATATTTGAAAATAACAATCAATCACATTTGCGAG
TAAGTTACTAATATATCTTGGTTATTGTTTAATAATTATGTAAAGTTATT
TTCAAATAAATTGTAAATGCAGTAAAAAAAAA
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protein sequence of SMED30031976-orf-1

>SMED30031976-orf-1 ID=SMED30031976-orf-1|Name=SMED30031976-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=272bp
MTTCRLTLIGSLSENQNSCDTPNLPKHIIIHDEPDEIVTIGRENSKTKVN
YVLGDSSKPRVVSRLHATLKYNADDLSFWLSDQSVNGTYVNYVRVPVSGL
KLEMEDIVCFFHRNGANIKPGEKVEPFDTHLKYRVEFVDEDDENLDEDEA
QSKSSKEPLKTSKKRGSFILDRSCSPETIAKKPRTKNSSSGKIRLGNIKN
TKRSSKSKVELVQDEEELCAAEICMRPIGLAIDWIQCDICSSWYHQMCLG
ITTAKAIDPSKPYYCPNCRSK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0010468regulation of gene expression
GO:0032259methylation
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR042803TCF19
IPR000253FHA_dom
IPR001965Znf_PHD
IPR019787Znf_PHD-finger
IPR013083Znf_RING/FYVE/PHD
IPR011011Znf_FYVE_PHD
IPR008984SMAD_FHA_dom_sf
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0008270zinc ion binding
GO:0008168methyltransferase activity
GO:0046872metal ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001965Zinc finger, PHD-typeSMARTSM00249PHD_3coord: 218..269
e-value: 3.3E-7
score: 39.9
IPR000253Forkhead-associated (FHA) domainSMARTSM00240FHA_2coord: 37..95
e-value: 1.1E-8
score: 44.8
IPR000253Forkhead-associated (FHA) domainPFAMPF00498FHAcoord: 38..106
e-value: 9.1E-10
score: 38.7
IPR000253Forkhead-associated (FHA) domainPROSITEPS50006FHA_DOMAINcoord: 38..95
score: 15.527
IPR000253Forkhead-associated (FHA) domainCDDcd00060FHAcoord: 20..96
e-value: 7.72031E-11
score: 55.8554
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 216..271
e-value: 5.6E-16
score: 59.8
NoneNo IPR availableGENE3DG3DSA:2.60.200.20coord: 24..149
e-value: 1.5E-13
score: 52.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 149..165
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 142..165
NoneNo IPR availableCDDcd15610PHD3_KDM5A_likecoord: 219..268
e-value: 1.1079E-14
score: 64.656
IPR019787Zinc finger, PHD-fingerPFAMPF00628PHDcoord: 232..270
e-value: 7.4E-10
score: 38.6
IPR019787Zinc finger, PHD-fingerPROSITEPS50016ZF_PHD_2coord: 216..271
score: 9.134
IPR042803Transcription factor 19PANTHERPTHR15464FAMILY NOT NAMEDcoord: 172..265
coord: 29..172
IPR011011Zinc finger, FYVE/PHD-typeSUPERFAMILYSSF57903FYVE/PHD zinc fingercoord: 193..269
IPR008984SMAD/FHA domain superfamilySUPERFAMILYSSF49879SMAD/FHA domaincoord: 28..113