TP53-regulating kinase

Overview
NameTP53-regulating kinase
Smed IDSMED30031932
Length (bp)921
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of TP53-regulating kinase (SMED30031932) t-SNE clustered cells

Violin plots show distribution of expression levels for TP53-regulating kinase (SMED30031932) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of TP53-regulating kinase (SMED30031932) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for TP53-regulating kinase (SMED30031932) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30031932

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30031932SMESG000016769.1 SmedASXL_000122SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of TP53-regulating kinase vs. Ensembl Human
Match: TP53RK (TP53 regulating kinase [Source:HGNC Symbol;Acc:HGNC:16197])

HSP 1 Score: 142.124 bits (357), Expect = 3.914e-40
Identity = 81/237 (34.18%), Postives = 132/237 (55.70%), Query Frame = 2 
Query:  116 KLIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMDFDRRIICMEYIFPSILVKEYLNILLSSNDELYEIPKQM--LACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSYFNGLPTSLRQI--VHRKLEHVRTRRRKKLAIG 814
            +L+KQGAEA+++   + G+ A+IK    K YRHP+L+  +  +R   E + L+   + G++ P ++ +D+    + ME I  S+ V++Y+     S  E  + P+ +  LA  IG+ L+ +H  ++IHGDLTTSN+L++               E++ +V IDFGL     LPED  VDL+V+E   LSTH    P  E +  ++     TS ++   V +KL+ VR R RK+  +G
Sbjct:   37 ELVKQGAEARVFRGRFQGRAAVIKHRFPKGYRHPALEARLGRRRTVQEARALLRCRRAGISAPVVFFVDYASNCLYMEEIEGSVTVRDYIQ----STMETEKTPQGLSNLAKTIGQVLARMHDEDLIHGDLTTSNMLLK------------PPLEQLNIVLIDFGLSFISALPEDKGVDLYVLEKAFLSTH----PNTETVFEAFLKSYSTSSKKARPVLKKLDEVRLRGRKRSMVG 253
[Close]
BLAST of TP53-regulating kinase vs. Ensembl Human
Match: TP53RK (TP53 regulating kinase [Source:HGNC Symbol;Acc:HGNC:16197])

HSP 1 Score: 49.6766 bits (117), Expect = 3.654e-7
Identity = 21/58 (36.21%), Postives = 35/58 (60.34%), Query Frame = 2 
Query:  119 LIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLG 292
            L+KQGAEA+++   + G+ A+IK    K YRHP+L+  +  +R   E + L+   + G
Sbjct:   38 LVKQGAEARVFRGRFQGRAAVIKHRFPKGYRHPALEARLGRRRTVQEARALLRCRRAG 95
[Close]
BLAST of TP53-regulating kinase vs. Ensembl Celegans
Match: F52C12.6 (pep chromosome:WBcel235:IV:1956711:1959749:1 gene:WBGene00138717.1 transcript:F52C12.6a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F52C12.6)

HSP 1 Score: 138.658 bits (348), Expect = 2.851e-39
Identity = 82/233 (35.19%), Postives = 127/233 (54.51%), Query Frame = 2 
Query:  119 LIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMDFDRRIICMEYIFPSILVKEYLNILLSSNDELYEIPKQMLACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQELFTKKAEEIPVVFIDFGLGLK-QVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSYFNGLPTSLRQIVHRKLEHVRTRRRKKLAIG 814
            L+ QGAEAK+ +  W G+ AIIKE  SK YRHP+LD  +   R + EI+ L  + +LG++VP +Y +D ++  + ME++ P    K +++ L   N   ++   +      G+ L  LH   +IHGDLTTSNI++R  ++ K             + FIDFGL  + +V PE+  VDL+V+E  ++STH      +E ++  Y           V +KL  +R R RK+  IG
Sbjct:   31 LLYQGAEAKVTKCIWLGRQAIIKERFSKGYRHPTLDTQLNKARTKQEIRGLNKARELGIHVPAVYFIDNEKNQLIMEFV-PGSTAKNWISQL---NPADFDAKTREFGQIFGEKLGKLHRGGLIHGDLTTSNIILRDDDLQK-------------MTFIDFGLSSQGKVTPEEKGVDLYVLERAVISTHDKCAALIEGLMEGYKKADGKQF-VAVEKKLNEIRLRGRKRDMIG 245
[Close]
BLAST of TP53-regulating kinase vs. Ensembl Celegans
Match: F52C12.6 (pep chromosome:WBcel235:IV:1958023:1959684:1 gene:WBGene00138717.1 transcript:F52C12.6b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F52C12.6)

HSP 1 Score: 73.559 bits (179), Expect = 7.054e-16
Identity = 50/158 (31.65%), Postives = 78/158 (49.37%), Query Frame = 2 
Query:  344 MEYIFPSILVKEYLNILLSSNDELYEIPKQMLACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQELFTKKAEEIPVVFIDFGLGLK-QVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSYFNGLPTSLRQIVHRKLEHVRTRRRKKLAIG 814
            ME++ P    K +++ L   N   ++   +      G+ L  LH   +IHGDLTTSNI++R  ++ K             + FIDFGL  + +V PE+  VDL+V+E  ++STH      +E ++  Y           V +KL  +R R RK+  IG
Sbjct:    1 MEFV-PGSTAKNWISQL---NPADFDAKTREFGQIFGEKLGKLHRGGLIHGDLTTSNIILRDDDLQK-------------MTFIDFGLSSQGKVTPEEKGVDLYVLERAVISTHDKCAALIEGLMEGYKKADGKQF-VAVEKKLNEIRLRGRKRDMIG 140
[Close]
BLAST of TP53-regulating kinase vs. Ensembl Fly
Match: Prpk (gene:FBgn0035590 transcript:FBtr0077180)

HSP 1 Score: 135.576 bits (340), Expect = 3.116e-38
Identity = 86/235 (36.60%), Postives = 120/235 (51.06%), Query Frame = 2 
Query:  116 KLIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMDFDRRIICMEYIFPSILVKEYLNILLSSNDELYEIPKQML--ACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSYFNGLPTSLRQIVHRKLEHVRTRRRKKLAIG 814
            +++KQGAE ++Y   + G+  +IKE   K YRHP L+  I  QRM+ E K        G+  P I H D +   + MEY   +   K+++   +S+  E  E  K +L    +IG+ +  +HSN++IHGDLTTSNILI              K  +  V+ IDFGL       ED  VDL+V+E  LLSTH       E +L++Y         Q V  K E VR R RK+  IG
Sbjct:    4 EILKQGAEGRLYLGDFKGEACLIKERFVKKYRHPELNTQITRQRMKAEAKASGRCLAAGILAPKILHSDLNTHKLYMEYFDAAKTAKQFIQETVSTQTE-DEAKKCLLEFCTRIGEIIGKMHSNHIIHGDLTTSNILI------------NPKGGDYDVILIDFGLSHYNQATEDKGVDLYVLERALLSTHSEQPYIFEHVLAAYRTACGKD-EQAVLTKFEEVRARGRKRTMIG 224
[Close]
BLAST of TP53-regulating kinase vs. Ensembl Zebrafish
Match: tp53rk (TP53 regulating kinase [Source:ZFIN;Acc:ZDB-GENE-050522-123])

HSP 1 Score: 139.813 bits (351), Expect = 1.093e-39
Identity = 86/237 (36.29%), Postives = 136/237 (57.38%), Query Frame = 2 
Query:  104 IEEAKLIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMDFDRRIICMEYIFPSILVKEYLNILLSSNDELYEIPKQMLACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSYFNGLPTSLRQIVHRKLEHVRTRRRKKLAIG 814
            ++EA++IKQGAEA++Y  T+ G+  IIKE   K YRHP +D+ +  +R   E++ ++   + G++ P +Y +D+    I ++ +  S+ V++++    S  D L     Q LA +IG+ L+ +H  ++IHGDLTTSN+L  LT   + Q+          +V IDFGL     LPED  VDL+V+E   LSTH   +   E +L SY +   +     V +KL+ VR R RK+  +G
Sbjct:   15 LKEAQMIKQGAEARVYRSTFLGRSVIIKERFPKLYRHPEVDEKLTRRRTTQEVRSILRCRRAGISAPVVYFVDYATHCIFLQDVVNSVSVRDHIR---SQPDGL-----QTLAERIGEVLAQMHDEDVIHGDLTTSNML--LTSGGQQQDF--------NLVLIDFGLSYISALPEDKGVDLYVLEKAFLSTHPKTEALFERLLKSYSS--ASKKSTAVIKKLDEVRLRGRKRSMVG 231
[Close]
BLAST of TP53-regulating kinase vs. Ensembl Xenopus
Match: TP53RK (TP53 regulating kinase [Source:NCBI gene;Acc:100127196])

HSP 1 Score: 139.813 bits (351), Expect = 1.972e-39
Identity = 83/232 (35.78%), Postives = 127/232 (54.74%), Query Frame = 2 
Query:  119 LIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMDFDRRIICMEYIFPSILVKEYLNILLSSNDELYEIPKQMLACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSYFNGLPTSLRQIVHRKLEHVRTRRRKKLAIG 814
            L+KQGAEA++Y   + GK A++KE   K YRHP+LD  +  +R   E++ +V   + G++ P +Y +D+    I +E I  S  V++++  +     E   +    LA KIG+ L+ +H  ++IHGDLTTSN+L+R               ++  +V IDFGL     LPED  VDL+V+E   LSTH   +     +L SY +    S    V +KL+ VR R RK+  +G
Sbjct:   22 LMKQGAEARVYRGRFLGKAAVVKERFPKAYRHPALDGKLTHRRTAQEVRSIVRCRKAGISAPVVYFVDYVTNCIYLEDIEESTTVRDHIISMQQCGKEASNLC--ALADKIGQVLARMHDEDVIHGDLTTSNMLLR------------PPCDDHNLVLIDFGLSFISALPEDKGVDLYVLEKAFLSTHPNTEEIFRALLQSYSSTSKKS--GPVIKKLDEVRLRGRKRSMVG 237
[Close]
BLAST of TP53-regulating kinase vs. Ensembl Mouse
Match: Trp53rka (transformation related protein 53 regulating kinase A [Source:MGI Symbol;Acc:MGI:1918294])

HSP 1 Score: 139.813 bits (351), Expect = 1.838e-39
Identity = 80/235 (34.04%), Postives = 130/235 (55.32%), Query Frame = 2 
Query:  116 KLIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMDFDRRIICMEYIFPSILVKEYLNILLSSNDELYEIPKQM--LACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSYFNGLPTSLRQIVHRKLEHVRTRRRKKLAIG 814
            +L++QGAEA+++   + G+ A++K    K+YRHP L+  +  +R   E + L+   + G+  P ++ +D+    + ME I  S+ V++Y+     S  E  + P+ +  LA ++G+ L+ +H  ++IHGDLTTSN+L+R            +   ++ +V IDFGL     LPED  VDL+V+E   LSTH   +   E  L SY  G  T     V +KL+ VR R RK+  +G
Sbjct:   28 ELVQQGAEARVFRGRFQGRAAVVKHRFPKSYRHPELEARLGRRRTVQEARALLRCRRAGIAAPVVFFVDYASNCLYMEEIEDSVTVRDYIQ----STMETEKDPQCLLDLARRMGQVLAGMHDQDLIHGDLTTSNMLLR------------RPLAQLHIVLIDFGLSFVSGLPEDKGVDLYVLEKAFLSTHPHTETVFEAFLKSY--GASTQKSSPVLKKLDEVRLRGRKRSMVG 244
[Close]
BLAST of TP53-regulating kinase vs. Ensembl Mouse
Match: Trp53rkb (transformation related protein 53 regulating kinase B [Source:MGI Symbol;Acc:MGI:1914050])

HSP 1 Score: 137.887 bits (346), Expect = 8.235e-39
Identity = 79/235 (33.62%), Postives = 130/235 (55.32%), Query Frame = 2 
Query:  116 KLIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMDFDRRIICMEYIFPSILVKEYLNILLSSNDELYEIPKQM--LACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSYFNGLPTSLRQIVHRKLEHVRTRRRKKLAIG 814
            +L++QGAEA+++   + G+ A++K    K+YRHP L+  +  +R   E + L+   + G+  P ++ +D+    + ME I  S+ V++Y+     S  E  + P+ +  LA ++G+ L+ +H  ++IHGDLTTSN+L+R            +   ++ +V IDFGL     LPED  VDL+V+E   LSTH   +   E  L SY  G  +     V +KL+ VR R RK+  +G
Sbjct:   28 ELVQQGAEARVFRGRFQGRAAVVKHRFPKSYRHPELEARLGRRRTVQEARALLRCRRAGIAAPVVFFVDYASNCLYMEEIEDSVTVRDYIQ----STMETEKDPQCLLDLARRMGQVLAGMHDQDLIHGDLTTSNMLLR------------RPLAQLHIVLIDFGLSFVSGLPEDKGVDLYVLEKAFLSTHPHTETAFEAFLKSY--GASSKKSSPVLKKLDEVRLRGRKRSMVG 244
[Close]
BLAST of TP53-regulating kinase vs. Ensembl Mouse
Match: Trp53rkb (transformation related protein 53 regulating kinase B [Source:MGI Symbol;Acc:MGI:1914050])

HSP 1 Score: 50.0618 bits (118), Expect = 3.432e-7
Identity = 19/59 (32.20%), Postives = 36/59 (61.02%), Query Frame = 2 
Query:  116 KLIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLG 292
            +L++QGAEA+++   + G+ A++K    K+YRHP L+  +  +R   E + L+   + G
Sbjct:   52 ELVQQGAEARVFRGRFQGRAAVVKHRFPKSYRHPELEARLGRRRTVQEARALLRCRRAG 110
[Close]
BLAST of TP53-regulating kinase vs. UniProt/SwissProt
Match: sp|Q96S44|PRPK_HUMAN (EKC/KEOPS complex subunit TP53RK OS=Homo sapiens OX=9606 GN=TP53RK PE=1 SV=2)

HSP 1 Score: 142.124 bits (357), Expect = 1.880e-39
Identity = 81/237 (34.18%), Postives = 132/237 (55.70%), Query Frame = 2 
Query:  116 KLIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMDFDRRIICMEYIFPSILVKEYLNILLSSNDELYEIPKQM--LACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSYFNGLPTSLRQI--VHRKLEHVRTRRRKKLAIG 814
            +L+KQGAEA+++   + G+ A+IK    K YRHP+L+  +  +R   E + L+   + G++ P ++ +D+    + ME I  S+ V++Y+     S  E  + P+ +  LA  IG+ L+ +H  ++IHGDLTTSN+L++               E++ +V IDFGL     LPED  VDL+V+E   LSTH    P  E +  ++     TS ++   V +KL+ VR R RK+  +G
Sbjct:   37 ELVKQGAEARVFRGRFQGRAAVIKHRFPKGYRHPALEARLGRRRTVQEARALLRCRRAGISAPVVFFVDYASNCLYMEEIEGSVTVRDYIQ----STMETEKTPQGLSNLAKTIGQVLARMHDEDLIHGDLTTSNMLLK------------PPLEQLNIVLIDFGLSFISALPEDKGVDLYVLEKAFLSTH----PNTETVFEAFLKSYSTSSKKARPVLKKLDEVRLRGRKRSMVG 253
[Close]
BLAST of TP53-regulating kinase vs. UniProt/SwissProt
Match: sp|Q54W07|BUD32_DICDI (EKC/KEOPS complex subunit bud32 OS=Dictyostelium discoideum OX=44689 GN=bud32 PE=3 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 1.890e-39
Identity = 94/232 (40.52%), Postives = 126/232 (54.31%), Query Frame = 2 
Query:  119 LIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMDFDRRIICMEYIFPSILVKEYLNILLSSNDELYEIPKQMLACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSYFNGLPTSLRQIVHRKLEHVRTRRRKKLAIG 814
            LI QGAEAK YE   +G   I+KE  SK YRHP LD+ I S+R+  E++ L    + G+ VP++Y +D     I ME+I     VK YL     S     +I   M   ++G  + ++H  N+IHGDLTTSN+L+R    +   EL          VFIDFGL       ED AVDL+V+E   +STH   +   + ILS+Y   L +S  +IV +KL  VR R RKK   G
Sbjct:   40 LISQGAEAKTYETDLYGLKCIVKERFSKAYRHPILDQKISSKRILQEVRSLNKCKKKGIQVPSLYLVDIGNNRIYMEFI-KGETVKHYLYKNQESTQHQNQIESIMK--ELGNQIGIIHEMNVIHGDLTTSNMLLR----ESTNEL----------VFIDFGLSYTSNSVEDKAVDLYVLERAFISTHPNSEQLFQTILSNY--ELTSSNSKIVIQKLNQVRLRGRKKTCFG 252
[Close]
BLAST of TP53-regulating kinase vs. UniProt/SwissProt
Match: sp|Q99PW4|PRPK_MOUSE (EKC/KEOPS complex subunit Tp53rk OS=Mus musculus OX=10090 GN=Tp53rk PE=2 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 5.404e-38
Identity = 79/235 (33.62%), Postives = 130/235 (55.32%), Query Frame = 2 
Query:  116 KLIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMDFDRRIICMEYIFPSILVKEYLNILLSSNDELYEIPKQM--LACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSYFNGLPTSLRQIVHRKLEHVRTRRRKKLAIG 814
            +L++QGAEA+++   + G+ A++K    K+YRHP L+  +  +R   E + L+   + G+  P ++ +D+    + ME I  S+ V++Y+     S  E  + P+ +  LA ++G+ L+ +H  ++IHGDLTTSN+L+R            +   ++ +V IDFGL     LPED  VDL+V+E   LSTH   +   E  L SY  G  +     V +KL+ VR R RK+  +G
Sbjct:   28 ELVQQGAEARVFRGRFQGRAAVVKHRFPKSYRHPELEARLGRRRTVQEARALLRCRRAGIAAPVVFFVDYASNCLYMEEIEDSVTVRDYIQ----STMETEKDPQCLLDLARRMGQVLAGMHDQDLIHGDLTTSNMLLR------------RPLAQLHIVLIDFGLSFVSGLPEDKGVDLYVLEKAFLSTHPHTETAFEAFLKSY--GASSKKSSPVLKKLDEVRLRGRKRSMVG 244
[Close]
BLAST of TP53-regulating kinase vs. UniProt/SwissProt
Match: sp|A1CLD2|BUD32_ASPCL (EKC/KEOPS complex subunit bud32 OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) OX=344612 GN=bud32 PE=3 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 1.930e-31
Identity = 91/262 (34.73%), Postives = 136/262 (51.91%), Query Frame = 2 
Query:  116 KLIKQGAEAKIYEITW--HGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMD---------FDRRIICMEYIFPSILVKEYL---------NILLSSNDELYEIPKQM--LACKIGKDLSLLHSNNMIHGDLTTSNILIR----LTEVDKYQELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSY---FNGLPTSLRQIVHRKLEHVRTRRRKKLAIG 814
            +L+ QGAEA +Y+  +     PA +K   SK YRHP LD+ +  QR+  E +CLV   + G+NVP +  +D         F    + ME++   ++V+  L         N   S  +EL E    +  L  +IG+ +  LH   +IHGDLTTSN+++R    + E     E  +  + E  VV IDFGL  + +  ED AVDL+V+E    STH   +PF E +L+ Y   + G  ++L     ++LE VR R RK+  IG
Sbjct:   24 QLLTQGAEAHLYKTVFLSPSTPAALKVRPSKPYRHPILDRRLTRQRILQEARCLVKLVREGVNVPAVLALDWEGQNTEKGFGGAWLMMEWV-EGLVVRVVLERWERWMKRNQGSSGAEELKEEENWVRDLLRRIGRAVGALHKAGVIHGDLTTSNLILRPPGHVGEQADTGE--SSPSMEGDVVLIDFGLASQSLQDEDRAVDLYVLERAFGSTHPRTEPFFEEVLNGYRESYKGASSAL-----KRLEDVRMRGRKRSMIG 277
[Close]
BLAST of TP53-regulating kinase vs. UniProt/SwissProt
Match: sp|Q4WYU4|BUD32_ASPFU (EKC/KEOPS complex subunit bud32 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=bud32 PE=3 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 6.775e-31
Identity = 88/259 (33.98%), Postives = 129/259 (49.81%), Query Frame = 2 
Query:  116 KLIKQGAEAKIYEITW--HGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMD---------FDRRIICMEYIFPSIL----------VKEYLNILLSSNDELYEIPKQMLACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQELF--TKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSY---FNGLPTSLRQIVHRKLEHVRTRRRKKLAIG 814
            +L+ QGAEA +Y+  +     PA +K   SK YRHP LD+ +  QR+  E +CLV   + G+NVP +  +D         F    + ME+I   ++          +K     L +   E  E   + L  +IG  +  LH   +IHGDLTTSN+++R     + Q     T  + E  VV IDFGL  + +  ED AVDL+V+E    STH   +P    +LS Y   + G  ++L     ++LE VR R RK+  IG
Sbjct:   24 QLLTQGAEAHLYKTVFLSPSAPAALKVRPSKPYRHPILDRRLTRQRILQEARCLVKLVREGVNVPAVLALDWEGQSGEKGFGGAWLMMEWIEGMVVRVVLERWEKYMKRNQAGLGAEEFEKEEARVRDLMKRIGHAVGALHKAGVIHGDLTTSNLILRPPTRAEQQPAADETNPSMEGDVVLIDFGLASQSLQDEDRAVDLYVLERAFGSTHPRTEPLFTEVLSGYKESYKGASSAL-----KRLEEVRMRGRKRSMIG 277
[Close]
BLAST of TP53-regulating kinase vs. TrEMBL
Match: A0A4Y2DXN8 (EKC/KEOPS complex subunit TP53RK OS=Araneus ventricosus OX=182803 GN=TP53RK PE=4 SV=1)

HSP 1 Score: 173.711 bits (439), Expect = 3.236e-49
Identity = 93/233 (39.91%), Postives = 138/233 (59.23%), Query Frame = 2 
Query:  116 KLIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMDFDRRIICMEYIFPSILVKEYLNILLSSNDELYEIPKQMLACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSYFNGLPTSLRQIVHRKLEHVRTRRRKKLAIG 814
            +LI QG+EAK+Y+  + GKP+I+KE   K YRHP LDK +  +R+R E++ L    + G+  P +Y  D + R I ++ I  SI VKEYL  +L + D +       LA KIG  ++ +H N ++HGD+TTSNIL++    DK  E  +  A++  V FIDFGL  + ++ ED A+DL+V+E  + S+H     F   IL  Y   +P +  + V  KLE VR R RK+  +G
Sbjct:   26 QLISQGSEAKVYKGEFLGKPSILKERFVKKYRHPELDKSLTCERIRAEVRALNKCHEAGIKCPAVYFADLESRCIILQEITNSITVKEYLKSILENQDSIEIDTLSPLARKIGSSIAKMHKNELVHGDITTSNILLK---TDKLNEFSS--ADDYEVYFIDFGLAKRDIVAEDKAIDLYVLERVISSSHPNADKFYTAILGEYMR-VPGNQSKAVADKLEEVRARGRKRTMVG 252
[Close]
BLAST of TP53-regulating kinase vs. TrEMBL
Match: A0A482VYN2 (Pkinase and/or Kdo domain containing protein OS=Asbolus verrucosus OX=1661398 GN=BDFB_003564 PE=4 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 4.434e-49
Identity = 94/237 (39.66%), Postives = 142/237 (59.92%), Query Frame = 2 
Query:  104 IEEAKLIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMDFDRRIICMEYIFPSILVKEYLNILLSSNDELYEIPKQMLACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSYFNGLPTSLRQIVHRKLEHVRTRRRKKLAIG 814
            +E  KL KQGAE +I++  + GKPAI KE   K YRHP LD  +  +R+++E + ++     G+  P +Y +DF+RRII MEY   SI VK+++  +   +DE+ +     LA KIG  L  +H+NN+IHGDLT+SN+L+    V+K ++      +++ ++FIDFGL   +   ED  VDL+V+E  L+STH       E +L SY N   +  ++ V  K E VR R RK+  +G
Sbjct:    1 MEGFKLFKQGAEGRIFKGVYLGKPAIAKERFVKKYRHPDLDSQLTKERIKSESRAIIRCKNAGIRTPTLYLVDFNRRIIFMEYFEKSIAVKDFIPQV---SDEVID----KLAVKIGTALGKMHANNIIHGDLTSSNMLL----VNKNEQPVYSDFQDLDLIFIDFGLAHVESGAEDKGVDLYVLERALISTHSTAVQIFEKVLESYQNENKSGFKE-VFSKFEEVRARGRKRTMVG 225
[Close]
BLAST of TP53-regulating kinase vs. TrEMBL
Match: A0A1S3H2B9 (TP53-regulating kinase-like OS=Lingula unguis OX=7574 GN=LOC106151518 PE=4 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 7.581e-49
Identity = 94/237 (39.66%), Postives = 142/237 (59.92%), Query Frame = 2 
Query:  104 IEEAKLIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMDFDRRIICMEYIFPSILVKEYLNILLSSNDELYEIPKQMLACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSYFNGLPTSLRQIVHRKLEHVRTRRRKKLAIG 814
            + +A L+KQGAEAKIY I + GKP I+KE   KTYRHP LDK + +QR ++EI+ ++     G+  P IY +D  + +I MEYI  S+ V++Y+  + ++ D    + + ++  K+G  L+ +H  N+IHGDLTTSN+L+R               EE+ ++ IDFGLG  + L ED  VDL+V+E   LSTH   +   E++L SY         +++ RKL+ VR R RK+  +G
Sbjct:    4 LSKATLLKQGAEAKIYVIDFLGKPCIVKERFQKTYRHPDLDKSLTAQRTKSEIRSMLRCRTNGIRTPVIYFVDQIKNLIYMEYIQDSVTVRDYVTAIQNNPDGDTHLLEALMQ-KVGSTLATMHEKNIIHGDLTTSNMLLR------------NPPEELNLILIDFGLGYIENLAEDKGVDLYVLERAFLSTHPNTEALFELLLQSYAKDYGKGAVEVL-RKLDEVRQRGRKRTMVG 226
[Close]
BLAST of TP53-regulating kinase vs. TrEMBL
Match: A0A0P5FEP4 (TP53-regulating kinase OS=Daphnia magna OX=35525 GN=APZ42_011819 PE=4 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 4.507e-46
Identity = 93/232 (40.09%), Postives = 132/232 (56.90%), Query Frame = 2 
Query:  119 LIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMDFDRRIICMEYIFPSILVKEYLNILLSSNDELYEIPKQMLACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSYFNGLPTSLRQIVHRKLEHVRTRRRKKLAIG 814
            LIKQG+EAKIY  ++HGKP I+KE   K YRHP LD+ I +QR++NE++ LV     G++ P +Y ++ +   I ME+I   + VK+Y+       D   +     LA  IG  + LLHS N+IHGDLTTSN+LIR  ++ K             +  IDFGL       ED  VDL+V+E  +LSTH   +P  E+IL SY        ++ + +KL+ +R R RK+   G
Sbjct:   10 LIKQGSEAKIYSGSFHGKPCIVKERFPKRYRHPHLDREISTQRLKNEVRSLVRCRLAGVHAPTVYCVNMELNHIMMEHIVDGVTVKDYI------KDPTSKSNLDNLASTIGATIGLLHSQNIIHGDLTTSNMLIRSGDISK-------------LTMIDFGLSFIDHSAEDKGVDLYVLERAMLSTHPNSEPLFEIILKSYLKTNKKDGKETL-KKLDEIRLRGRKRSMAG 221
[Close]
BLAST of TP53-regulating kinase vs. TrEMBL
Match: V9LAM3 (TP53-regulating kinase OS=Callorhinchus milii OX=7868 PE=2 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 4.888e-46
Identity = 93/233 (39.91%), Postives = 136/233 (58.37%), Query Frame = 2 
Query:  116 KLIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMDFDRRIICMEYIFPSILVKEYLNILLSSNDELYEIPKQMLACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSYFNGLPTSLRQIVHRKLEHVRTRRRKKLAIG 814
            ++IKQGAEAKIY +T+ G+P I+K   SKTYRHP+LDK +  +R   E++ ++   + G++ P +Y +D++   I +E +  S+ V++Y+        E   +    LA KIG+ L+ +H  N+IHGDLTTSNIL++            K  +E  +V IDFGL     LPED  VDL+V+E   LSTH   +   E+IL SY     TS    V +KL+ VR R RK+  +G
Sbjct:   27 QIIKQGAEAKIYRVTFLGRPTIVKARFSKTYRHPALDKKLTHRRTAQEVRSILRCRKAGISTPVVYFVDYNSNSIYLEDLVGSVSVRDYITSTWQEKKETVSL--YPLAEKIGQILAKMHDQNVIHGDLTTSNILLQ------------KPEDEQSLVMIDFGLSFISALPEDKGVDLYVLEKAFLSTHPNTESVFEVILKSYSASSKTS--GPVIKKLDEVRQRGRKRSMVG 243
[Close]
BLAST of TP53-regulating kinase vs. Ensembl Cavefish
Match: tp53rk (TP53 regulating kinase [Source:NCBI gene;Acc:103042064])

HSP 1 Score: 141.354 bits (355), Expect = 3.132e-40
Identity = 84/237 (35.44%), Postives = 131/237 (55.27%), Query Frame = 2 
Query:  113 AKLIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMDFDRRIICMEYIFPSILVKEYLNILLSSN---DELYEIPKQMLACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSYFNGLPTSLRQIVHRKLEHVRTRRRKKLAIG 814
            A+L+KQGAEA++Y   + GK  I+KE   K YRHP LD+ +  +R   E++ ++   + G++ P +Y +D+    I +E I  SI ++E++    +S    + LY      LA +IG+ L+ +H  +++HGDLTTSN+L+   E  K  +L          V IDFGL     LPED  VD++V+E   LSTH   +   + +L SY     +     V +KL+ VR R RK+  +G
Sbjct:   20 AQLLKQGAEARVYRGRFLGKSTIVKERFPKRYRHPVLDEKLTKRRTAQEVRSILRCRKAGISAPVVYFVDYTTNCIFLEEITDSITIREHITSTQASGRSTETLYG-----LADRIGQILAKMHDEDVVHGDLTTSNMLLVGEEDGKPGKL----------VLIDFGLSYISALPEDKGVDMYVLEKAFLSTHPNTEALFQRLLKSYIAA--SKKAPAVIKKLDEVRLRGRKRSMVG 239
[Close]
BLAST of TP53-regulating kinase vs. Ensembl Sea Lamprey
Match: tp53rk (TP53 regulating kinase [Source:ZFIN;Acc:ZDB-GENE-050522-123])

HSP 1 Score: 138.658 bits (348), Expect = 9.487e-40
Identity = 81/233 (34.76%), Postives = 127/233 (54.51%), Query Frame = 2 
Query:  119 LIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMDFDRRIICMEYIFPSILVKEYLNILLSSNDELYEIPKQM-LACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSYFNGLPTSLRQIVHRKLEHVRTRRRKKLAIG 814
            LI QGAEA++Y  T+ G+PA++KE   K YRHP LD  +  +R   E++ L+   + G+  P +Y +D++R  I ME +  ++ V+EY+    S++       K + LA  IG+ L+ +H+ +++HGDLTTSN+L+  T+      L            IDFGL     L ED AVDL V+E   LS+H   +     +L++Y   +       +  KL+ VR R RK+  +G
Sbjct:   37 LISQGAEARVYRGTFLGRPAVLKERFPKKYRHPQLDWRLTRRRTVQEVRSLLRCRRAGILTPVVYFVDYERHCIYMEDLAGAVTVREYVQ---STSQAAAGGEKLLTLAVLIGRTLAHMHAQDVVHGDLTTSNLLLVPTQPTPRMAL------------IDFGLSCVSTLVEDKAVDLHVLEKAFLSSHPGTEAMFAAVLAAYTETMKGRFAAPIIAKLDEVRLRGRKRSMVG 254
[Close]
BLAST of TP53-regulating kinase vs. Ensembl Yeast
Match: BUD32 (Protein kinase; component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; Pyrococcus Bud32 ortholog functions as a P-loop ATPase rather than a protein kinase in the context of the complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; mutation is functionally complemented by human TP53RK [Source:SGD;Acc:S000003494])

HSP 1 Score: 82.8037 bits (203), Expect = 4.920e-19
Identity = 80/254 (31.50%), Postives = 111/254 (43.70%), Query Frame = 2 
Query:  122 IKQGAEAKIYEITWHG---------KPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQL-GLNVPNIYHMDFDRRIICMEYIFPSILVKEYL----NILLSSNDELYEIPKQMLACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTH--HLGK-------PFLEMILSSYFNGLPTSLRQIVHRKLEHVRTRRRKKLAIG 814
            I QGAEA ++  T H          +  IIK    K YRHP +D+ +   R  NE + L     + GL VP +   D     I +E++   +          N L   + + Y         K+G+ + LLH N+  HGDLT+SNI+           L    A   P + IDFGLG    L ED  VDL+V+E  +LSTH  H  K        F E+       G    L+++  R  E VR R RK+  +G
Sbjct:   22 ISQGAEAIVFTTTTHPYLPRAKDSHQKYIIKYRPPKRYRHPQIDQALTKHRTLNESRLLAKLYLIPGLCVPQLIACDPYNGFIWLEFLGEDLPGGHGFSNLKNFLWMHDQDPYSDLVATTLRKVGRQIGLLHWNDYCHGDLTSSNIV-----------LVRDGARWTPHL-IDFGLGSVSNLVEDKGVDLYVLERAILSTHSKHAEKYNAWIMEGFEEVYREQGAKGA-KKLKEVTKR-FEEVRLRGRKRSMLG 261
[Close]
BLAST of TP53-regulating kinase vs. Ensembl Nematostella
Match: EDO33026 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7STD0])

HSP 1 Score: 145.206 bits (365), Expect = 2.285e-42
Identity = 83/243 (34.16%), Postives = 132/243 (54.32%), Query Frame = 2 
Query:  101 MIEEAKLIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMDFDRRIICMEYIFPSILVKEYLNIL-----LSSNDELYEIPKQMLACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSYFNGLPTSLRQIVHRKLEHVRTRRRKKLAIG 814
            M++E +L+KQGAEAKI+   ++ +P ++K+   K YRHPSLD+ +  +R   E++ ++   ++G+  P +Y +D++   I ME I   + V++ +N L     L + ++LYE+ K+     IG  L+ +H  + IHGDLTTSN+L               K     +  IDFGL     L ED  VDL+V+E   LSTH   +   E IL +Y      +   I  +KL+ +R R RK+  +G
Sbjct:    1 MVDEWELVKQGAEAKIFFTEFYNRPTVVKQRFCKCYRHPSLDEKLTRRRTTQEVRSMLRCRKVGITTPTVYFVDYEAFCIYMERIEDCMTVRDVVNGLLEDSSLQATEKLYELAKE-----IGVLLAKMHQVDCIHGDLTTSNLL--------------SKNSSNAITIIDFGLSSVSSLAEDKGVDLYVLERAFLSTHPNTEKIFEAILDAYRKNARNATEVI--KKLDEIRMRGRKRTMVG 222
[Close]
BLAST of TP53-regulating kinase vs. Ensembl Medaka
Match: tp53rk (TP53 regulating kinase [Source:NCBI gene;Acc:101166289])

HSP 1 Score: 139.043 bits (349), Expect = 1.543e-39
Identity = 85/243 (34.98%), Postives = 133/243 (54.73%), Query Frame = 2 
Query:   98 QMIEEAKLIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMDFDRRIICMEYIFPSILVKEYL----NILLSSNDELYEIPKQMLACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSYFNGLPTSLRQIVHRKLEHVRTRRRKKLAIG 814
            Q++  A+++KQGAE ++Y   + GKPA+IK+   K YRHP LD+ +  +R   E++ +    + G++ P +Y +D+    I +E I  S  V++++         S+ EL     Q LA   G+ L+ +H  ++IHGDLTTSN+L+R           +  + E+ +V IDFGL     L ED  VDL+V+E   LSTH   +P  + +L SY     +     V +KLE VR R RK+  +G
Sbjct:    5 QLLRGAEMLKQGAEGRVYRAEFLGKPAVIKQRFPKRYRHPELDEKLTHRRTMQEVRSIARCRRAGISTPVVYFVDYSCSCIFLEEIVDSQTVRDFIASARRSSSRSDQEL-----QRLAELTGRILAKMHDEDVIHGDLTTSNMLLRR----------SADSSELELVLIDFGLSYISALAEDKGVDLYVLEKAFLSTHPNTEPLFQQLLGSYSA--SSKKSSAVIKKLEEVRLRGRKRSMVG 230
[Close]
BLAST of TP53-regulating kinase vs. Planmine SMEST
Match: SMESG000016769.1 (SMESG000016769.1)

HSP 1 Score: 485.723 bits (1249), Expect = 1.025e-175
Identity = 236/238 (99.16%), Postives = 238/238 (100.00%), Query Frame = 2 
Query:  101 MIEEAKLIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMDFDRRIICMEYIFPSILVKEYLNILLSSNDELYEIPKQMLACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSYFNGLPTSLRQIVHRKLEHVRTRRRKKLAIG 814
            MIEEAKLIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMDFDRRIICMEYIFPSILVKEYLNILLSSNDELYEIPKQMLACKIGKDLSLLHSNNMIHGDLTTSN+LI+LTEVDKYQELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSYFNGLPTSLRQIVHRKLEHVRTRRRKKLAIG
Sbjct:    1 MIEEAKLIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRMRNEIKCLVWSSQLGLNVPNIYHMDFDRRIICMEYIFPSILVKEYLNILLSSNDELYEIPKQMLACKIGKDLSLLHSNNMIHGDLTTSNMLIKLTEVDKYQELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPFLEMILSSYFNGLPTSLRQIVHRKLEHVRTRRRKKLAIG 238
The following BLAST results are available for this feature:
BLAST of TP53-regulating kinase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
TP53RK3.914e-4034.18TP53 regulating kinase [Source:HGNC Symbol;Acc:HGN... [more]
TP53RK3.654e-736.21TP53 regulating kinase [Source:HGNC Symbol;Acc:HGN... [more]
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BLAST of TP53-regulating kinase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
F52C12.62.851e-3935.19pep chromosome:WBcel235:IV:1956711:1959749:1 gene:... [more]
F52C12.67.054e-1631.65pep chromosome:WBcel235:IV:1958023:1959684:1 gene:... [more]
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BLAST of TP53-regulating kinase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Prpk3.116e-3836.60gene:FBgn0035590 transcript:FBtr0077180[more]
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BLAST of TP53-regulating kinase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tp53rk1.093e-3936.29TP53 regulating kinase [Source:ZFIN;Acc:ZDB-GENE-0... [more]
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BLAST of TP53-regulating kinase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
TP53RK1.972e-3935.78TP53 regulating kinase [Source:NCBI gene;Acc:10012... [more]
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BLAST of TP53-regulating kinase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Trp53rka1.838e-3934.04transformation related protein 53 regulating kinas... [more]
Trp53rkb8.235e-3933.62transformation related protein 53 regulating kinas... [more]
Trp53rkb3.432e-732.20transformation related protein 53 regulating kinas... [more]
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BLAST of TP53-regulating kinase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q96S44|PRPK_HUMAN1.880e-3934.18EKC/KEOPS complex subunit TP53RK OS=Homo sapiens O... [more]
sp|Q54W07|BUD32_DICDI1.890e-3940.52EKC/KEOPS complex subunit bud32 OS=Dictyostelium d... [more]
sp|Q99PW4|PRPK_MOUSE5.404e-3833.62EKC/KEOPS complex subunit Tp53rk OS=Mus musculus O... [more]
sp|A1CLD2|BUD32_ASPCL1.930e-3134.73EKC/KEOPS complex subunit bud32 OS=Aspergillus cla... [more]
sp|Q4WYU4|BUD32_ASPFU6.775e-3133.98EKC/KEOPS complex subunit bud32 OS=Neosartorya fum... [more]
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BLAST of TP53-regulating kinase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4Y2DXN83.236e-4939.91EKC/KEOPS complex subunit TP53RK OS=Araneus ventri... [more]
A0A482VYN24.434e-4939.66Pkinase and/or Kdo domain containing protein OS=As... [more]
A0A1S3H2B97.581e-4939.66TP53-regulating kinase-like OS=Lingula unguis OX=7... [more]
A0A0P5FEP44.507e-4640.09TP53-regulating kinase OS=Daphnia magna OX=35525 G... [more]
V9LAM34.888e-4639.91TP53-regulating kinase OS=Callorhinchus milii OX=7... [more]
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BLAST of TP53-regulating kinase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tp53rk3.132e-4035.44TP53 regulating kinase [Source:NCBI gene;Acc:10304... [more]
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BLAST of TP53-regulating kinase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tp53rk9.487e-4034.76TP53 regulating kinase [Source:ZFIN;Acc:ZDB-GENE-0... [more]
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BLAST of TP53-regulating kinase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
BUD324.920e-1931.50Protein kinase; component of the EKC/KEOPS complex... [more]
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BLAST of TP53-regulating kinase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO330262.285e-4234.16Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of TP53-regulating kinase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tp53rk1.543e-3934.98TP53 regulating kinase [Source:NCBI gene;Acc:10116... [more]
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BLAST of TP53-regulating kinase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000016769.11.025e-17599.16SMESG000016769.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031932 ID=SMED30031932|Name=TP53-regulating kinase|organism=Schmidtea mediterranea sexual|type=transcript|length=921bp
GTTAATTGAAATATTTAAATATTCACATTAATATAATTTTGATCATTTGT
TTAAATATTCAGTTTCAAGTTCTAGTTTGAAATTATAGATTTCAAAACAG
ATGATAGAAGAAGCGAAGTTAATTAAGCAAGGAGCAGAAGCAAAGATTTA
TGAAATAACATGGCATGGTAAACCAGCAATTATAAAAGAAGTTCTTTCTA
AAACATATCGACATCCTTCATTGGATAAGTTAATTATCTCTCAGAGAATG
AGAAATGAAATTAAATGTCTAGTTTGGTCTTCACAACTAGGATTAAATGT
TCCAAATATTTATCACATGGATTTTGATCGTAGAATAATATGTATGGAAT
ATATTTTTCCTTCCATATTAGTTAAAGAATATTTAAATATTCTTTTGTCA
TCAAATGATGAATTATATGAAATTCCTAAACAAATGCTTGCTTGTAAGAT
TGGAAAGGATTTATCATTGCTTCATTCTAATAATATGATCCATGGAGATT
TAACAACCTCAAATATACTTATTAGACTAACTGAAGTTGATAAATATCAA
GAATTATTTACAAAGAAAGCTGAAGAAATACCAGTAGTGTTTATTGATTT
TGGACTCGGCTTGAAACAAGTTTTACCAGAAGATCTTGCAGTTGATCTGT
TTGTCATGGAGTCTACTTTGTTAAGCACCCATCATTTAGGCAAGCCATTT
TTGGAAATGATTTTATCTTCCTACTTTAATGGACTACCAACAAGTCTCAG
GCAAATTGTGCATAGGAAACTTGAACACGTACGCACGAGACGACGAAAGA
AACTTGCAATTGGATAAATTTATTAATAAATTTTATTTTACGATTAGATT
GATATTTTTTAATTAATCACATTTTGTACCGTCATTGTAATTTTACATGA
ATAGATATGTTCAAATATTGG
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protein sequence of SMED30031932-orf-1

>SMED30031932-orf-1 ID=SMED30031932-orf-1|Name=SMED30031932-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=239bp
MIEEAKLIKQGAEAKIYEITWHGKPAIIKEVLSKTYRHPSLDKLIISQRM
RNEIKCLVWSSQLGLNVPNIYHMDFDRRIICMEYIFPSILVKEYLNILLS
SNDELYEIPKQMLACKIGKDLSLLHSNNMIHGDLTTSNILIRLTEVDKYQ
ELFTKKAEEIPVVFIDFGLGLKQVLPEDLAVDLFVMESTLLSTHHLGKPF
LEMILSSYFNGLPTSLRQIVHRKLEHVRTRRRKKLAIG*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR022495Bud32
IPR001245Ser-Thr/Tyr_kinase_cat_dom
IPR000719Prot_kinase_dom
IPR008266Tyr_kinase_AS
IPR011009Kinase-like_dom_sf
Vocabulary: biological process
TermDefinition
GO:0006468protein phosphorylation
GO:0016310phosphorylation
GO:0070525tRNA threonylcarbamoyladenosine metabolic process
GO:0008033tRNA processing
Vocabulary: molecular function
TermDefinition
GO:0004672protein kinase activity
GO:0005524ATP binding
GO:0016740transferase activity
GO:0016787hydrolase activity
GO:0000166nucleotide binding
GO:0004674protein serine/threonine kinase activity
GO:0016301kinase activity
Vocabulary: cellular component
TermDefinition
GO:0000408EKC/KEOPS complex
GO:0005634nucleus
GO:0005829cytosol
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.10.510.10coord: 104..235
e-value: 1.2E-21
score: 79.0
NoneNo IPR availableGENE3DG3DSA:3.30.200.20coord: 13..85
e-value: 1.5E-21
score: 77.6
NoneNo IPR availablePANTHERPTHR12209:SF0EKC/KEOPS COMPLEX SUBUNIT TP53RKcoord: 3..237
NoneNo IPR availableCDDcd00180PKccoord: 10..169
e-value: 9.05273E-8
score: 48.8059
IPR022495Serine/threonine-protein kinase Bud32TIGRFAMTIGR03724TIGR03724coord: 7..232
e-value: 1.5E-43
score: 146.6
IPR022495Serine/threonine-protein kinase Bud32PANTHERPTHR12209O-SIALOGLYCOPROTEIN ENDOPEPTIDASEcoord: 3..237
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714Pkinase_Tyrcoord: 5..145
e-value: 1.9E-5
score: 24.1
IPR008266Tyrosine-protein kinase, active sitePROSITEPS00109PROTEIN_KINASE_TYRcoord: 129..141
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 2..238
score: 11.241
IPR011009Protein kinase-like domain superfamilySUPERFAMILYSSF56112Protein kinase-like (PK-like)coord: 5..186