S-like RNase

Overview


Name	S-like RNase
Smed ID	SMED30031930
Length (bp)	1042

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of S-like RNase (SMED30031930) t-SNE clustered cells

Violin plots show distribution of expression levels for S-like RNase (SMED30031930) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of S-like RNase (SMED30031930) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for S-like RNase (SMED30031930) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30031930

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 6

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
intestinal phagocyte	SMED30031930	SMESG000016389.1	Contig758	GPL15192	PMID:23079596 Forsthoefel et al., 2012
gut	SMED30031930	SMESG000016389.1	dd_Smed_v4_1008_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30031930	SMESG000016389.1	dd_Smed_v4_1008_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30031930	SMESG000016389.1	dd_Smed_v4_1008_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30031930	SMESG000016389.1	Contig39931	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30031930	SMESG000016389.1	Contig39931	uc_Smed_v2	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Homology

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BLAST of S-like RNase vs. Ensembl Human
Match: AL159163.1 (novel protein)

HSP 1 Score: 81.2629 bits (199), Expect = 1.144e-17
Identity = 45/131 (34.35%), Postives = 67/131 (51.15%), Query Frame = 1

Query:  121 WDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGDPFNINDLNSIYPDLVVQWANLL-EFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPS 510
            W  L     WP+T C+  Q N  +     W IHGLWP    G + S     PFN+ ++  + P++   W +++  F N + FW HEW KHG CA       ++  YF RSL+L + + L+ +L    I+PS
Sbjct:   15 WKKLIMVQHWPETVCEKIQ-NDCRDPPDYWTIHGLWPDKSEGCNRSW----PFNLEEIKDLLPEMRAYWPDVIHSFPNRSRFWKHEWEKHGTCAAQVDALNSQKKYFGRSLELYRELDLNSVLLKLGIKPS 140

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BLAST of S-like RNase vs. Ensembl Human
Match: RNASET2 (ribonuclease T2 [Source:HGNC Symbol;Acc:HGNC:21686])

HSP 1 Score: 82.0333 bits (201), Expect = 2.527e-17
Identity = 44/132 (33.33%), Postives = 67/132 (50.76%), Query Frame = 1

Query:  118 NWDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGDPFNINDLNSIYPDLVVQWANLL-EFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPS 510
             W  L     WP+T C+  Q N  +     W IHGLWP    G +    +  PFN+ ++  + P++   W +++  F N + FW HEW KHG CA       ++  YF RSL+L + + L+ +L    I+PS
Sbjct:   33 EWKKLIMVQHWPETVCEKIQ-NDCRDPPDYWTIHGLWPDKSEGCN----RSWPFNLEEIKDLLPEMRAYWPDVIHSFPNRSRFWKHEWEKHGTCAAQVDALNSQKKYFGRSLELYRELDLNSVLLKLGIKPS 159

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Query:  118 NWDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGDPFNINDLNSIYPDLVVQWANLL-EFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPS 510
             W  L     WP+T C+  Q N  +     W IHGLWP    G +    +  PFN+ ++  + P++   W +++  F N + FW HEW KHG CA       ++  YF RSL+L + + L+ +L    I+PS
Sbjct:   33 EWKKLIMVQHWPETVCEKIQ-NDCRDPPDYWTIHGLWPDKSEGCN----RSWPFNLEEIKDLLPEMRAYWPDVIHSFPNRSRFWKHEWEKHGTCAAQVDALNSQKKYFGRSLELYRELDLNSVLLKLGIKPS 159

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BLAST of S-like RNase vs. Ensembl Human
Match: RNASET2 (ribonuclease T2 [Source:HGNC Symbol;Acc:HGNC:21686])

HSP 1 Score: 81.6481 bits (200), Expect = 2.711e-17
Identity = 44/132 (33.33%), Postives = 67/132 (50.76%), Query Frame = 1

Query:  118 NWDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGDPFNINDLNSIYPDLVVQWANLL-EFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPS 510
             W  L     WP+T C+  Q N  +     W IHGLWP    G +    +  PFN+ ++  + P++   W +++  F N + FW HEW KHG CA       ++  YF RSL+L + + L+ +L    I+PS
Sbjct:   33 EWKKLIMVQHWPETVCEKIQ-NDCRDPPDYWTIHGLWPDKSEGCN----RSWPFNLEEIKDLLPEMRAYWPDVIHSFPNRSRFWKHEWEKHGTCAAQVDALNSQKKYFGRSLELYRELDLNSVLLKLGIKPS 159

[Close]

BLAST of S-like RNase vs. Ensembl Human
Match: RNASET2 (ribonuclease T2 [Source:HGNC Symbol;Acc:HGNC:21686])

HSP 1 Score: 79.337 bits (194), Expect = 1.186e-16
Identity = 42/122 (34.43%), Postives = 65/122 (53.28%), Query Frame = 1

Query:  148 WPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGDPFNINDLNSIYPDLVVQWANLL-EFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPS 510
            WP+T C+  Q N  +     W IHGLWP    G +    +  PFN+ ++  + P++   W +++  F N + FW HEW KHG CA       ++  YF RSL+L + + L+ +L    I+PS
Sbjct:    5 WPETVCEKIQ-NDCRDPPDYWTIHGLWPDKSEGCN----RSWPFNLEEIKDLLPEMRAYWPDVIHSFPNRSRFWKHEWEKHGTCAAQVDALNSQKKYFGRSLELYRELDLNSVLLKLGIKPS 121

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BLAST of S-like RNase vs. Ensembl Celegans
Match: rnst-2 (pep chromosome:WBcel235:V:1061595:1067908:1 gene:WBGene00019624.1 transcript:K10C9.3.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:rnst-2)

HSP 1 Score: 91.2781 bits (225), Expect = 6.665e-21
Identity = 61/209 (29.19%), Postives = 97/209 (46.41%), Query Frame = 1

Query:  121 WDYLTYTLLWPQTFCKYNQCNYDKT-----KVTGWNIHGLWPSNITGKDPSKCKGDP--FNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECI--KIEDDYYLSAVEICFDHQLKPINCPK-HHHDNVDSQLKDLIHQ 717
            +DYL +T ++P   C+ +  +  ++         W+IHGLWP+   G  P  C+G P  F+ N + SI   LVV W NL        FW HE+ KHG CA    LF +EL+YF+  +++  F S+D       + PS+     +D  +       G   +  C+  K    + L  + +C +  L   +CP    H N  S+ +  I +
Sbjct:   23 FDYLMFTTIYPTAVCRADDDSVPESCEIPSGTPQWSIHGLWPNFENGSYPQNCRGTPRHFDENLIKSIEDRLVVVWPNLYPKKTIQSFWKHEYDKHGTCAQSEKLFESELAYFTEVMKV--FDSIDVAGGLKSVGPSEKPITSSDLKNALSGVTSGKTFQFHCLRDKKTKQFLLGDIRLCLNKDLTIRDCPTDGKHPNRVSRFERSIGR 229

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BLAST of S-like RNase vs. Ensembl Fly
Match: RNaseX25 (gene:FBgn0010406 transcript:FBtr0076752)

HSP 1 Score: 86.2705 bits (212), Expect = 8.856e-19
Identity = 61/192 (31.77%), Postives = 91/192 (47.40%), Query Frame = 1

Query:  118 NWDYLTYTLLWPQTFCKY-------NQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGDP-FNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECI--KIEDDYYLSAVEICFDHQLKPINC 663
            NWD L +T  WP T C +        +C+  + K   W IHG+WP+ +    P+ C     F+ + LN I   L   W +L    +    W HEW KHG CA+L     NEL YF + L  ++   + +IL  +DI P  D      +++ ++   LG  P + C+        YLS + ICF   L+ I+C
Sbjct:   75 NWDVLIFTQQWPVTTCYHWREENPDQECSLPQKK-EFWTIHGIWPTKLHQMGPNFCNNSANFDPSKLNPIEDRLETFWPDLKGMDSTEWLWKHEWQKHGTCAMLVEELDNELKYFEQGLTWREEYIMSRILDASDIHP--DSNNTVAAINNAIVKALGKNPSIHCLYDGKHGISYLSEIRICFSKSLELIDC 263

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BLAST of S-like RNase vs. Ensembl Zebrafish
Match: rnaset2 (ribonuclease T2 [Source:NCBI gene;Acc:791890])

HSP 1 Score: 95.5153 bits (236), Expect = 1.433e-22
Identity = 77/228 (33.77%), Postives = 112/228 (49.12%), Query Frame = 1

Query:  115 ANWDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGD-PFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECIKIEDD---YYLSAVEICFDHQLKPINCPKHHHDNVDSQLKDLIHQIFQKVEGHLLLS--DATKPINIP 780
              W  L  T  WPQTFCK   C   KT  + W +HGLWP+  TG    +C     FN + +  I P++   W +LLE ++P +FW +EWTKHG CA       +E  YF ++L+L     L+ +L  N I PS +K Y  + V ++++S  GV PK++C+          L  +EIC D   + + C K   D   + L  +       V G   LS  D + P+  P
Sbjct:   25 GEWTKLILTQHWPQTFCKMEHC---KTDFSYWTLHGLWPN--TG---VRCNTSWHFNASLIEDILPEMEKFWPDLLEPSSP-KFWNYEWTKHGTCAAKSESLNSEHKYFGKALELYHKFDLNSVLLKNQIVPS-EKHYTLEDVEEAITSAYGVKPKIQCVHPGQGGQVQILGQIEICVDRDFQLMGCEKSSEDTWSNDLPTV------PVSGQSGLSVCDHSMPVYYP 236

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Query:  115 ANWDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGD-PFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECIKIEDD---YYLSAVEICFDHQLKPINCPKHHHDNVDSQLKDLIHQIFQKVEGHLLLS--DATKPINIP 780
              W  L  T  WPQTFCK   C   KT  + W +HGLWP+  TG    +C     FN + +  I P++   W +LLE ++P +FW +EWTKHG CA       +E  YF ++L+L     L+ +L  N I PS +K Y  + V ++++S  GV PK++C+          L  +EIC D   + + C K   D   + L  +       V G   LS  D + P+  P
Sbjct:   25 GEWTKLILTQHWPQTFCKMEHC---KTDFSYWTLHGLWPN--TG---VRCNTSWHFNASLIEDILPEMEKFWPDLLEPSSP-KFWNYEWTKHGTCAAKSESLNSEHKYFGKALELYHKFDLNSVLLKNQIVPS-EKHYTLEDVEEAITSAYGVKPKIQCVHPGQGGQVQILGQIEICVDRDFQLMGCEKSSEDTWSNDLPTV------PVSGQSGLSVCDHSMPVYYP 236

[Close]

Query:  115 ANWDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGD-PFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECIKIEDD---YYLSAVEICFDHQLKPINCPKHHHDNVDSQLKDLIHQIFQKVEGHLLLS--DATKPINIP 780
              W  L  T  WPQTFCK   C   KT  + W +HGLWP+  TG    +C     FN + +  I P++   W +LLE ++P +FW +EWTKHG CA       +E  YF ++L+L     L+ +L  N I PS +K Y  + V ++++S  GV PK++C+          L  +EIC D   + + C K   D   + L  +       V G   LS  D + P+  P
Sbjct:   25 GEWTKLILTQHWPQTFCKMEHC---KTDFSYWTLHGLWPN--TG---VRCNTSWHFNASLIEDILPEMEKFWPDLLEPSSP-KFWNYEWTKHGTCAAKSESLNSEHKYFGKALELYHKFDLNSVLLKNQIVPS-EKHYTLEDVEEAITSAYGVKPKIQCVHPGQGGQVQILGQIEICVDRDFQLMGCEKSSEDTWSNDLPTV------PVSGQSGLSVCDHSMPVYYP 236

[Close]

Query:  115 ANWDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGD-PFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECIKIEDD---YYLSAVEICFDHQLKPINCPKHHHDNVDSQLKDLIHQIFQKVEGHLLLS--DATKPINIP 780
              W  L  T  WPQTFCK   C   KT  + W +HGLWP+  TG    +C     FN + +  I P++   W +LLE ++P +FW +EWTKHG CA       +E  YF ++L+L     L+ +L  N I PS +K Y  + V ++++S  GV PK++C+          L  +EIC D   + + C K   D   + L  +       V G   LS  D + P+  P
Sbjct:   25 GEWTKLILTQHWPQTFCKMEHC---KTDFSYWTLHGLWPN--TG---VRCNTSWHFNASLIEDILPEMEKFWPDLLEPSSP-KFWNYEWTKHGTCAAKSESLNSEHKYFGKALELYHKFDLNSVLLKNQIVPS-EKHYTLEDVEEAITSAYGVKPKIQCVHPGQGGQVQILGQIEICVDRDFQLMGCEKSSEDTWSNDLPTV------PVSGQSGLSVCDHSMPVYYP 236

[Close]

Query:  115 ANWDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGD-PFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECIKIEDD---YYLSAVEICFDHQLKPINCPKHHHDNVDSQLKDLIHQIFQKVEGHLLLS--DATKPINIP 780
              W  L  T  WPQTFCK   C   KT  + W +HGLWP+  TG    +C     FN + +  I P++   W +LLE ++P +FW +EWTKHG CA       +E  YF ++L+L     L+ +L  N I PS +K Y  + V ++++S  GV PK++C+          L  +EIC D   + + C K   D   + L  +       V G   LS  D + P+  P
Sbjct:   25 GEWTKLILTQHWPQTFCKMEHC---KTDFSYWTLHGLWPN--TG---VRCNTSWHFNASLIEDILPEMEKFWPDLLEPSSP-KFWNYEWTKHGTCAAKSESLNSEHKYFGKALELYHKFDLNSVLLKNQIVPS-EKHYTLEDVEEAITSAYGVKPKIQCVHPGQGGQVQILGQIEICVDRDFQLMGCEKSSEDTWSNDLPTV------PVSGQSGLSVCDHSMPVYYP 236

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BLAST of S-like RNase vs. Ensembl Xenopus
Match: rnaset2 (ribonuclease T2 [Source:NCBI gene;Acc:780173])

HSP 1 Score: 69.3218 bits (168), Expect = 3.579e-13
Identity = 47/152 (30.92%), Postives = 67/152 (44.08%), Query Frame = 1

Query:  208 WNIHGLWPSNITGKDPSKCKGDPFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECIKIEDDYYLSAVEICFDHQLKPINC 663
            W +HGLWP      + S     PF  +++  I P+L   W ++L   N ++ W HEW KHG CA        +  YFS+ L+L   V L+ +L  + I PS  K Y                    C   E+   L  +EICF  +L+  NC
Sbjct:   60 WTLHGLWPDKAQMCNNSW----PFEYSEIQDILPELNHYWPDILH-PNKSQLWKHEWQKHGTCAASLECLNTQHKYFSKGLELYTKVDLNSVLEKSGIVPS-TKYYQV-----------------RCYLGENVQTLGQIEICFTKELQLRNC 188

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BLAST of S-like RNase vs. Ensembl Mouse
Match: Rnaset2a (ribonuclease T2A [Source:MGI Symbol;Acc:MGI:1915445])

HSP 1 Score: 76.2554 bits (186), Expect = 1.875e-15
Identity = 43/132 (32.58%), Postives = 67/132 (50.76%), Query Frame = 1

Query:  118 NWDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGDPFNINDLNSIYPDLVVQWANLL-EFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPS 510
             W  L  T  WP T CK  + N  +  +  W IHGLWP      + S      FN++++  +  D+ + W +++   +N ++FW HEW KHG CA       +E  YF +SL L + + L+ +L    I+PS
Sbjct:   38 EWKKLILTQHWPPTVCK--EVNSCQDSLDYWTIHGLWPDRAEDCNQSW----HFNLDEIKDLLRDMKIYWPDVIHRSSNRSQFWKHEWVKHGTCAAQVDALNSEKKYFGKSLDLYKQIDLNSVLQKFGIKPS 163

[Close]

Query:  118 NWDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGDPFNINDLNSIYPDLVVQWANLL-EFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPS 510
             W  L  T  WP T CK  + N  +  +  W IHGLWP      + S      FN++++  +  D+ + W +++   +N ++FW HEW KHG CA       +E  YF +SL L + + L+ +L    I+PS
Sbjct:   38 EWKKLILTQHWPPTVCK--EVNSCQDSLDYWTIHGLWPDRAEDCNQSW----HFNLDEIKDLLRDMKIYWPDVIHRSSNRSQFWKHEWVKHGTCAAQVDALNSEKKYFGKSLDLYKQIDLNSVLQKFGIKPS 163

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BLAST of S-like RNase vs. Ensembl Mouse
Match: Gm49673 (predicted gene, 49673 [Source:MGI Symbol;Acc:MGI:6215117])

HSP 1 Score: 76.2554 bits (186), Expect = 1.875e-15
Identity = 43/132 (32.58%), Postives = 67/132 (50.76%), Query Frame = 1

Query:  118 NWDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGDPFNINDLNSIYPDLVVQWANLL-EFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPS 510
             W  L  T  WP T CK  + N  +  +  W IHGLWP      + S      FN++++  +  D+ + W +++   +N ++FW HEW KHG CA       +E  YF +SL L + + L+ +L    I+PS
Sbjct:   38 EWKKLILTQHWPPTVCK--EVNSCQDSLDYWTIHGLWPDRAEDCNQSW----HFNLDEIKDLLRDMKIYWPDVIHRSSNRSQFWKHEWVKHGTCAAQVDALNSEKKYFGKSLDLYKQIDLNSVLQKFGIKPS 163

[Close]

BLAST of S-like RNase vs. Ensembl Mouse
Match: Gm49721 (predicted gene, 49721 [Source:MGI Symbol;Acc:MGI:6215195])

HSP 1 Score: 76.2554 bits (186), Expect = 1.875e-15
Identity = 43/132 (32.58%), Postives = 67/132 (50.76%), Query Frame = 1

Query:  118 NWDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGDPFNINDLNSIYPDLVVQWANLL-EFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPS 510
             W  L  T  WP T CK  + N  +  +  W IHGLWP      + S      FN++++  +  D+ + W +++   +N ++FW HEW KHG CA       +E  YF +SL L + + L+ +L    I+PS
Sbjct:   38 EWKKLILTQHWPPTVCK--EVNSCQDSLDYWTIHGLWPDRAEDCNQSW----HFNLDEIKDLLRDMKIYWPDVIHRSSNRSQFWKHEWVKHGTCAAQVDALNSEKKYFGKSLDLYKQIDLNSVLQKFGIKPS 163

[Close]

BLAST of S-like RNase vs. Ensembl Mouse
Match: Rnaset2b (ribonuclease T2B [Source:MGI Symbol;Acc:MGI:3702087])

HSP 1 Score: 76.2554 bits (186), Expect = 1.875e-15
Identity = 43/132 (32.58%), Postives = 67/132 (50.76%), Query Frame = 1

Query:  118 NWDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGDPFNINDLNSIYPDLVVQWANLL-EFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPS 510
             W  L  T  WP T CK  + N  +  +  W IHGLWP      + S      FN++++  +  D+ + W +++   +N ++FW HEW KHG CA       +E  YF +SL L + + L+ +L    I+PS
Sbjct:   38 EWKKLILTQHWPPTVCK--EVNSCQDSLDYWTIHGLWPDRAEDCNQSW----HFNLDEIKDLLRDMKIYWPDVIHRSSNRSQFWKHEWVKHGTCAAQVDALNSEKKYFGKSLDLYKQIDLNSVLQKFGIKPS 163

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BLAST of S-like RNase vs. UniProt/SwissProt
Match: sp|Q7M456|RNOY_CRAGI (Ribonuclease Oy OS=Crassostrea gigas OX=29159 PE=1 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 6.647e-29
Identity = 67/189 (35.45%), Postives = 96/189 (50.79%), Query Frame = 1

Query:  118 NWDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNIT-GKDPSKCKGD-PFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECI---KIEDDYYLSAVEICFDHQLKPINCPK 669
            +W+Y T+   WP   C  ++  +    V GW IHGLWPS+ T  K P  C G  PF+IN++  + P+L   W NL   T    FW HEW+KHG CA   P  +NEL YF   L+L    ++ +IL +  I PS    Y  +    +V  +LGV   +EC+   +      L  + IC   + + I+C K
Sbjct:    2 DWNYFTFAQQWPIAVCAEHKSCFIPDSVVGWGIHGLWPSSDTESKGPENCNGSWPFDINNVMPLVPELKKYWPNLYPDTKANSFWEHEWSKHGTCATSLPATSNELKYFGMGLKLHAKYNISRILVNQGILPSKTAGYMINETEAAVKRELGVDAVIECVYDKEKTKKQLLYEISICLTKEFELISCNK 190

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BLAST of S-like RNase vs. UniProt/SwissProt
Match: sp|B8XY56|RNT2_DANRE (Ribonuclease T2 OS=Danio rerio OX=7955 GN=rnaset2 PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.082e-21
Identity = 77/228 (33.77%), Postives = 112/228 (49.12%), Query Frame = 1

Query:  115 ANWDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGD-PFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECIKIEDD---YYLSAVEICFDHQLKPINCPKHHHDNVDSQLKDLIHQIFQKVEGHLLLS--DATKPINIP 780
              W  L  T  WPQTFCK   C   KT  + W +HGLWP+  TG    +C     FN + +  I P++   W +LLE ++P +FW +EWTKHG CA       +E  YF ++L+L     L+ +L  N I PS +K Y  + V ++++S  GV PK++C+          L  +EIC D   + + C K   D   + L  +       V G   LS  D + P+  P
Sbjct:   25 GEWTKLILTQHWPQTFCKMEHC---KTDFSYWTLHGLWPN--TG---VRCNTSWHFNASLIEDILPEMEKFWPDLLEPSSP-KFWNYEWTKHGTCAAKSESLNSEHKYFGKALELYHKFDLNSVLLKNQIVPS-EKHYTLEDVEEAITSAYGVKPKIQCVHPGQGGQVQILGQIEICVDRDFQLMGCEKSSEDTWSNDLPTV------PVSGQSGLSVCDHSMPVYYP 236

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BLAST of S-like RNase vs. UniProt/SwissProt
Match: sp|O61887|RNST2_CAEEL (Ribonuclease T2 protein rnst-2 OS=Caenorhabditis elegans OX=6239 GN=rnst-2 PE=1 SV=2)

HSP 1 Score: 91.2781 bits (225), Expect = 8.478e-20
Identity = 61/209 (29.19%), Postives = 97/209 (46.41%), Query Frame = 1

Query:  121 WDYLTYTLLWPQTFCKYNQCNYDKT-----KVTGWNIHGLWPSNITGKDPSKCKGDP--FNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECI--KIEDDYYLSAVEICFDHQLKPINCPK-HHHDNVDSQLKDLIHQ 717
            +DYL +T ++P   C+ +  +  ++         W+IHGLWP+   G  P  C+G P  F+ N + SI   LVV W NL        FW HE+ KHG CA    LF +EL+YF+  +++  F S+D       + PS+     +D  +       G   +  C+  K    + L  + +C +  L   +CP    H N  S+ +  I +
Sbjct:   23 FDYLMFTTIYPTAVCRADDDSVPESCEIPSGTPQWSIHGLWPNFENGSYPQNCRGTPRHFDENLIKSIEDRLVVVWPNLYPKKTIQSFWKHEYDKHGTCAQSEKLFESELAYFTEVMKV--FDSIDVAGGLKSVGPSEKPITSSDLKNALSGVTSGKTFQFHCLRDKKTKQFLLGDIRLCLNKDLTIRDCPTDGKHPNRVSRFERSIGR 229

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BLAST of S-like RNase vs. UniProt/SwissProt
Match: sp|Q7M438|RNDI_DICDI (Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3)

HSP 1 Score: 85.5001 bits (210), Expect = 3.738e-18
Identity = 57/186 (30.65%), Postives = 95/186 (51.08%), Query Frame = 1

Query:  115 ANWDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGDPFNINDLNSIYPDLVVQWANLLEFTNP-AEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECIKIEDDYYLSAVEICFD-HQLKPINCP 666
             ++D+  +   W  ++C    C  +K +   + IHGLWP N  G  PS C G  FN+N +  +   L   W +L   T P  +FWTHE++KHG C+I  P+ T+   YF+  ++L    ++   L   +I PSD   Y    ++ ++++  G  P ++C   +    LS V +C D + L  ++CP
Sbjct:   30 GDFDFYLFVQQWIYSYCDSQTCIQNKER-EAFTIHGLWPENSDGSYPSFCSGPSFNVNAIQDLEDQLNFDWPSL---TGPNTDFWTHEFSKHGTCSITGPI-TDIHDYFATGIKLYTEFNITAALESENIYPSDSNTYKPVDITNAITTHFGGKPGIQCSSGQ----LSTVAVCIDKNSLSIMDCP 206

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BLAST of S-like RNase vs. UniProt/SwissProt
Match: sp|P42815|RNS3_ARATH (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 3.390e-17
Identity = 66/202 (32.67%), Postives = 101/202 (50.00%), Query Frame = 1

Query:  118 NWDYLTYTLLWPQTFC-KYNQCNYDKTK--VTGWNIHGLWPSNITGKDPSKCKGDP-FNINDLNSIYPDLVVQWANLLEFTNPA-EFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECIK-IEDDYYLSAVEICFDHQL-KPINCPKHHHDNVDSQLK 702
            ++D+  + L WP  +C   + C Y +T      + IHGLWP+  TG  P  C  D  F+   ++ +  DL  +W  L   +N   +FWTHEW KHG CA       ++  YF   L+LKQ  +L   L++  I+P DDK Y+   +  ++   +G  P +EC K    +  L  + +C D    K INCP   H   DS+++
Sbjct:   21 DFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESE---LDQHDYFEAGLKLKQKANLLHALTNAGIKP-DDKFYEMKDIENTIKQVVGFAPGIECNKDSSHNSQLYQIYLCVDTSASKFINCPVMPHGRCDSRVQ 218

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BLAST of S-like RNase vs. TrEMBL
Match: A0A4Z2DFD7 (Ribonuclease Oy isoform 2 OS=Schistosoma japonicum OX=6182 GN=EWB00_001528 PE=3 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 8.589e-29
Identity = 71/192 (36.98%), Postives = 102/192 (53.12%), Query Frame = 1

Query:  121 WDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGD-PFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECIKI-EDDYYLSAVEICFDHQLKPINCPKHHHDNVD 690
            WDYL Y+L WP T+C  + C      +  +NIHGLWPS      P+ C    PF I+ +  IY +L  +WANL +F +P  FW HEW KHG+CA+  P+ +NEL YF+ SL +K  V+L + L    I PSD      D +   +     V   + C  I      L+ + +C +  ++ I+CP   H N+D
Sbjct:   24 WDYLVYSLEWPPTYCFTHTCKL-PYNINNFNIHGLWPSIWPSGSPTNCPNHMPFEIDTIKPIYTELQKEWANLDDFDDPKAFWKHEWQKHGVCALSDPIISNELDYFNISLIMKSKVNLLRRLESIKIIPSDSVTLKRDVLLNQLKKLFNVDVLMYCFLIHHKPAKLAEIRLCLNPSMEFISCPSSIH-NID 213

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BLAST of S-like RNase vs. TrEMBL
Match: A0A4Z2DG04 (Ribonuclease Oy isoform 1 OS=Schistosoma japonicum OX=6182 GN=EWB00_001528 PE=3 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 1.283e-28
Identity = 71/192 (36.98%), Postives = 102/192 (53.12%), Query Frame = 1

Query:  121 WDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGD-PFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECIKI-EDDYYLSAVEICFDHQLKPINCPKHHHDNVD 690
            WDYL Y+L WP T+C  + C      +  +NIHGLWPS      P+ C    PF I+ +  IY +L  +WANL +F +P  FW HEW KHG+CA+  P+ +NEL YF+ SL +K  V+L + L    I PSD      D +   +     V   + C  I      L+ + +C +  ++ I+CP   H N+D
Sbjct:   44 WDYLVYSLEWPPTYCFTHTCKL-PYNINNFNIHGLWPSIWPSGSPTNCPNHMPFEIDTIKPIYTELQKEWANLDDFDDPKAFWKHEWQKHGVCALSDPIISNELDYFNISLIMKSKVNLLRRLESIKIIPSDSVTLKRDVLLNQLKKLFNVDVLMYCFLIHHKPAKLAEIRLCLNPSMEFISCPSSIH-NID 233

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BLAST of S-like RNase vs. TrEMBL
Match: G4V5B9 (Putative ribonuclease t2 OS=Schistosoma mansoni OX=6183 GN=Smp_158430 PE=3 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 6.691e-28
Identity = 74/210 (35.24%), Postives = 110/210 (52.38%), Query Frame = 1

Query:  121 WDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGD-PFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILP-PLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXEC-IKIEDDYYLSAVEICFDHQLKPINCPKHHHDNVDSQLKDLIHQIFQKVEGH 741
            WD+  ++L WP T+C    C    + +  +NIHGLWPS     +P  C    PF I  +  IY DL  QWANL++F NP +FW HEW+KHG+CAI   PL +NEL YF+ SL +K  V+L   L    I P++      D +   + +   V   + C ++      LS + IC +  L+ I+C     + ++ QL    +  ++ V GH
Sbjct:   24 WDHFVFSLEWPPTYCFIQTCKIPYS-INDFNIHGLWPSIWPHTEPKNCSNRTPFRIELIKPIYNDLQKQWANLIDFDNPEDFWKHEWSKHGVCAISDHPLISNELDYFNISLAIKSRVNLLSRLESVSITPNNLVTLKRDMLLDQLKNLFSVNVLIYCSMRKHQPGRLSEIRICLNPSLEFIDCFTSSANKINHQL--FSNHFYKTVYGH 230

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BLAST of S-like RNase vs. TrEMBL
Match: A0A4Z2DFD4 (Ribonuclease Oy OS=Schistosoma japonicum OX=6182 GN=EWB00_001525 PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 1.599e-27
Identity = 68/187 (36.36%), Postives = 100/187 (53.48%), Query Frame = 1

Query:  115 ANWDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKG-DPFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXEC-IKIEDDYY--LSAVEICFDHQLKPINC 663
             +W+Y  +TL WP TFC Y +C      +  + IHGLWPS   GK P+ C     F+I+ L SI   L   WANLL + NP+ FW HEW KHG C I   L +NEL+YF+ +++LK+ ++L   L    IQP++    +       +     V   ++C  K   D    L+ +  CF+ +L+ I+C
Sbjct:   26 GDWNYFVFTLTWPPTFCSYKKCKLPPG-LNDFTIHGLWPSIWPGKQPTNCSAHSRFDIHRLQSIRNKLDYTWANLLNYENPSPFWEHEWYKHGQCGIENVLISNELNYFNTAVELKEKLNLLTQLKSYGIQPNNSVVIEKSHFLNVLKQAYNVSAVVKCKSKRRKDKLTKLAEIRFCFNVKLQLIDC 211

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BLAST of S-like RNase vs. TrEMBL
Match: Q5BVY2 (SJCHGC07916 protein (Fragment) OS=Schistosoma japonicum OX=6182 PE=2 SV=2)

HSP 1 Score: 115.161 bits (287), Expect = 2.692e-27
Identity = 60/139 (43.17%), Postives = 80/139 (57.55%), Query Frame = 1

Query:  121 WDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGD-PFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTD 534
            WDYL Y+L WP T+C  + C      +  +NIHGLWPS      P+ C    PF I+ +  IY +L  +WANL +F +P  FW HEW KHG+CA+  P+ +NEL YF+ SL +K  V+L + L    I PSD      D
Sbjct:   24 WDYLVYSLEWPPTYCFTHTCKL-PYNINNFNIHGLWPSIWPSGSPTNCPNHMPFEIDTIKPIYTELQKEWANLDDFDDPKAFWKHEWQKHGVCALSDPIISNELDYFNISLIMKSKVNLLRRLESIKIIPSDSVTLKRD 161

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BLAST of S-like RNase vs. Ensembl Cavefish
Match: rnaset2 (ribonuclease T2 [Source:NCBI gene;Acc:103036121])

HSP 1 Score: 84.7297 bits (208), Expect = 1.259e-18
Identity = 62/187 (33.16%), Postives = 97/187 (51.87%), Query Frame = 1

Query:  118 NWDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGDPFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECI---KIEDDYYLSAVEICFDHQLKPINCPK 669
            +W  L  T  WP+TFC  +QC    T  + W +HG WP N+     S      FN + +  + P++   W +LL+  +  +FW HEW KHG CA       ++  YFS++L+L   + LD +L  + I PS +K Y    + +S+ S  GV PK++C+   K  +   +  +EIC D Q +  +C K
Sbjct:   25 DWTKLILTHHWPKTFCMIDQCT---TNFSYWTLHGFWP-NVGNMCNSSWH---FNASLIEDLLPEMKTYWPDLLKPAS-TQFWKHEWEKHGTCAAKDEDLDSQHKYFSKALELYHKLDLDGVLKKSQIVPS-EKYYKLADLEESIVSSFGVTPKIQCVLEAKRAEVQTVGQIEICVDKQFQLTDCEK 202

[Close]

BLAST of S-like RNase vs. Ensembl Cavefish
Match: rnaset2l (ribonuclease Oy-like [Source:NCBI gene;Acc:103022964])

HSP 1 Score: 76.2554 bits (186), Expect = 1.755e-15
Identity = 49/168 (29.17%), Postives = 78/168 (46.43%), Query Frame = 1

Query:  115 ANWDYLTYTLLWPQTFC----KYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGDPFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECIKIEDD 606
             NW  + +T+ WP +FC      N C   +  +  W IHGLWP    G     C   P   + L  + P+L   W +L++  +   FW  EW KHG CA       +   YF  SL+L+    +D  L++  I+PS +  Y  + +  ++   LG    ++C+K E +
Sbjct:   42 CNWTCMIFTIQWPGSFCLSLENRNICRIPE-NIHNWTIHGLWPIRTYGC----CFCWPVFQSHLQDLEPELSQLWPSLIKSKSNFSFWKDEWFKHGTCAACVESMGSPAHYFRTSLKLRTLFDVDNALANTGIKPSCNISYKHEEIHAALGPFLGNNYVVQCVKDEKE 204

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BLAST of S-like RNase vs. Ensembl Sea Lamprey
Match: rnaset2 (ribonuclease T2 [Source:NCBI gene;Acc:791890])

HSP 1 Score: 91.6633 bits (226), Expect = 9.600e-22
Identity = 58/198 (29.29%), Postives = 103/198 (52.02%), Query Frame = 1

Query:  118 NWDYLTYTLLWPQTFC-----KYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGDPFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECIKI---EDDYYLSAVEICFDHQLKPINCPKHHHDNV 687
            +W  L  T  WPQ+FC     + + C    + V  W+IHGLW ++ T  +    K   F++ ++  + P L   W +L E TN  + W HEW +HG CA       ++ +YFS++++L Q + + K LSD  + PS+   Y  + + ++ S+  GV  KL+C+ +   E+   L  +++C+  +   +NC +H    +
Sbjct:   28 DWSQLILTQHWPQSFCMSQWSEKHNCTIPSS-VKSWSIHGLWTNHRTFCN----KSWHFDVKEIEDLLPQLDQFWPDL-EKTNGTDLWKHEWIRHGTCAATLEALNSQHNYFSKTMELYQKLDISKALSDAGVTPSNTVFYKEEQILEAFSTAFGVKAKLQCLTVPQEENTQLLGQIQMCWSREFSLVNCSRHAEAGI 219

[Close]

BLAST of S-like RNase vs. Ensembl Nematostella
Match: EDO48543 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJ98])

HSP 1 Score: 98.9821 bits (245), Expect = 2.806e-24
Identity = 67/214 (31.31%), Postives = 105/214 (49.07%), Query Frame = 1

Query:  121 WDYLTYTLLWPQTFCKY--NQCNYDKTK--------------VTGWNIHGLWPSNITGKDPSKCKGD-PFNINDLNSIYPDLVVQWANLLEFTNPA--EFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECIKIEDD-YYLSAVEICFDHQLKPINC----PKHHHDNVD 690
            WDY  ++  WPQ+ C Y   Q   DK +              VT W +HGLWP+     +P  C    PF  +++  +   ++ +W    + +  +  + W+HEW KHG CA      +NE SYFS +L L     L + L+  +I PSDD+ Y    V +++S+K G   ++ C++  DD   L+ + IC D   +P NC    P+H  D+ D
Sbjct:   27 WDYFVFSQWWPQSQCYYRNGQTMDDKWRFTSGNARNDCVPADVTTWTLHGLWPTVGGKAEPVNCNSSWPFVESEIQDLEDRMMQRWLAFPDSSKSSARDLWSHEWKKHGTCATDLAQTSNEHSYFSMALALNSNCGLLRALASENIIPSDDQMYTVKQVERAISNKYGAKGRVICLRGPDDQQLLAGIRICLDKSFRPRNCIQTYPEHCSDDDD 240

[Close]

BLAST of S-like RNase vs. Ensembl Nematostella
Match: EDO48544 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJ99])

HSP 1 Score: 82.4185 bits (202), Expect = 2.878e-18
Identity = 49/154 (31.82%), Postives = 74/154 (48.05%), Query Frame = 1

Query:  121 WDYLTYTLLWPQTFCKY--NQCNYDKTK--------------VTGWNIHGLWPSNITGKDPSKCKGD-PFNINDLNSIYPDLVVQWANLLEFTNPA--EFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQY 525
            WDY  ++  WPQ+ C Y   Q   DK +              VT W +HGLWP+     +P  C    PF  +++  +   ++ +W    + +  +  + W+HEW KHG CA      +NE SYFS +L L     L + L+  +I PSDD+ Y
Sbjct:   20 WDYFVFSQWWPQSQCYYRNGQTMDDKWRFTSGNARNDCVPADVTTWTLHGLWPTVGGKAEPVNCNSSWPFVESEIQDLEDRMMQRWLAFPDSSKSSARDLWSHEWKKHGTCATDLAETSNEHSYFSMALALNSNCGLLRALASENIIPSDDQMY 173

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BLAST of S-like RNase vs. Ensembl Medaka
Match: rnaset2l (ribonuclease Oy [Source:NCBI gene;Acc:101170531])

HSP 1 Score: 88.9669 bits (219), Expect = 4.774e-20
Identity = 50/141 (35.46%), Postives = 72/141 (51.06%), Query Frame = 1

Query:  121 WDYLTYTLLWPQTFCK--YNQ--CNYDKTKVTGWNIHGLWPSNITGKDPSKCKGDPFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDT 531
            W  + +TL WP  FC   YNQ  C    T VT W IHGLWP +        C   P   +D+  +  +LV  W +LL+  +  +FW  EW KHG CA     F +   YF  SL+L+++  + ++L D  I PS +++Y  
Sbjct:   49 WRCIIFTLQWPGGFCASLYNQTLCRIPDT-VTDWTIHGLWPLHAQ----RCCNCWPMFQSDVQELKDELVENWPSLLKTKSSFQFWEQEWAKHGACAACVEGFNSPQKYFQISLKLRKWFEIQRVLEDAGIAPSCERRYKV 184

[Close]

Query:  121 WDYLTYTLLWPQTFCK--YNQ--CNYDKTKVTGWNIHGLWPSNITGKDPSKCKGDPFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDT 531
            W  + +TL WP  FC   YNQ  C    T VT W IHGLWP +        C   P   +D+  +  +LV  W +LL+  +  +FW  EW KHG CA     F +   YF  SL+L+++  + ++L D  I PS +++Y  
Sbjct:   49 WRCIIFTLQWPGGFCASLYNQTLCRIPDT-VTDWTIHGLWPLHAQ----RCCNCWPMFQSDVQELKDELVENWPSLLKTKSSFQFWEQEWAKHGACAACVEGFNSPQKYFQISLKLRKWFEIQRVLEDAGIAPSCERRYKV 184

[Close]

BLAST of S-like RNase vs. Ensembl Medaka
Match: rnaset2 (ribonuclease T2 [Source:NCBI gene;Acc:101163650])

HSP 1 Score: 80.8777 bits (198), Expect = 2.998e-17
Identity = 61/187 (32.62%), Postives = 89/187 (47.59%), Query Frame = 1

Query:  121 WDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGD-PFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECI---KIEDDYYLSAVEICFDHQLKPINCPK 669
            W  L  T  WP TFC    C+     ++ W +HGLWP    G D   C     FN + +  + PD+   W +L   ++   FW +EW KHG CA       ++  YFS++L+L   + L  +L    I PS DK Y    V  ++ +  G  PK++C+   K  D   L  +EICFD     ++C K
Sbjct:   27 WSKLILTHHWPNTFCSMEHCH---PNISYWTLHGLWPDK--GMD---CNSSWHFNPSLIEDLLPDMNKSWPDLRNRSS-VSFWKYEWHKHGTCAAKAESLNSQHKYFSKALELYHKLDLSSVLVKFGITPS-DKYYPFSQVEGAIENVYGFKPKIQCVHSSKGGDFQSLGQIEICFDSDFTLMDCEK 203

[Close]

Query:  121 WDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGD-PFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECI---KIEDDYYLSAVEICFDHQLKPINCPK 669
            W  L  T  WP TFC    C+     ++ W +HGLWP    G D   C     FN + +  + PD+   W +L   ++   FW +EW KHG CA       ++  YFS++L+L   + L  +L    I PS DK Y    V  ++ +  G  PK++C+   K  D   L  +EICFD     ++C K
Sbjct:   27 WSKLILTHHWPNTFCSMEHCH---PNISYWTLHGLWPDK--GMD---CNSSWHFNPSLIEDLLPDMNKSWPDLRNRSS-VSFWKYEWHKHGTCAAKAESLNSQHKYFSKALELYHKLDLSSVLVKFGITPS-DKYYPFSQVEGAIENVYGFKPKIQCVHSSKGGDFQSLGQIEICFDSDFTLMDCEK 203

[Close]

BLAST of S-like RNase vs. Planmine SMEST
Match: SMESG000016389.1 (SMESG000016389.1)

HSP 1 Score: 298.13 bits (762), Expect = 5.930e-102
Identity = 161/162 (99.38%), Postives = 162/162 (100.00%), Query Frame = 1

Query:  115 ANWDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGDPFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECIKIE 600
            ANWDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGDPFNINDLNSI+PDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDSVSKSVSSKLGVLPKLECIKIE
Sbjct:   17 ANWDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGDPFNINDLNSIFPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDSVSKSVSSKLGVLPKLECIKIE 178

[Close]

BLAST of S-like RNase vs. Planmine SMEST
Match: SMESG000046228.1 (SMESG000046228.1)

HSP 1 Score: 134.42 bits (337), Expect = 1.731e-37
Identity = 79/218 (36.24%), Postives = 127/218 (58.26%), Query Frame = 1

Query:  166 KYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGDPFNIN-DLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECIKIED-DYYLSAVEICFDHQLKPINCPKHHHDNVD--SQLKDLIHQIFQKVEGHLLLSDATKPINIPCNDNSFYIP 807
            KY+     ++K + WNIHGLWP  I G D   C G+PF     L SIY +L++ WANL  F+ P +FW +EW KHG C+I   LF+NE  YF  +L+LK+ +S+ K+LS+++I P++DK Y+   +  ++  +LG++P L+C + +D ++ L ++ ICFD  +   +C      NV+    +  +I  + +K+  +L       PI   C  + F IP
Sbjct:    6 KYHSSATIQSKDSRWNIHGLWPYKIHGNDTFNCDGEPFQRQLVLISIYEELLLNWANLKNFSKPEKFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAIKVELGIIPMLQCFQKQDNEWNLLSIGICFDRSMNLKDC-----QNVELPQSMGSIIGNLIKKIFLYLGAKKVITPIKDKCEGHDFKIP 218

[Close]

BLAST of S-like RNase vs. Planmine SMEST
Match: SMESG000048912.1 (SMESG000048912.1)

HSP 1 Score: 133.265 bits (334), Expect = 5.462e-37
Identity = 85/235 (36.17%), Postives = 120/235 (51.06%), Query Frame = 1

Query:  118 NWDYLTYTLLWPQTFC----KYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCK-GDPFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECIKIEDDYYLSAVEICFDHQLKPINCPKHHHDNVDSQLKDLIHQIFQKVEGHLLLSDATKPINIPCNDNSFYIP 807
            +WDYL   + WP +FC    K  QC  D + VTGWN+HGLWPS+  G DP  CK    ++   L  I  DL+ +W  L   +   +FW HEW  HG CA    L   EL YF+ ++Q+K+ V L  IL  N+++P ++K Y        +     V PKL+C   +   Y+ A+E CFD +L  I CPK H      + +D + +   K        ++T PI+IPC     Y P
Sbjct:    5 DWDYLVMAVEWPPSFCYTFPKKIQC-CDTSHVTGWNLHGLWPSSSKGSDPLCCKNASAYDEKKLKPIMEDLLKEWNPLGNCS--KDFWKHEWKFHGTCASHNKLLHTELQYFNETIQMKKHVDLTNILHQNNVKPDNNKMYKVSDFVNVIKKVYKVEPKLQCTHYKGKIYILAMEFCFDTKLNIIMCPKRH------KSEDHLSRHRNKCRK---PRNSTNPIHIPCTTGFLYQP 227

[Close]

BLAST of S-like RNase vs. Planmine SMEST
Match: SMESG000048912.1 (SMESG000048912.1)

HSP 1 Score: 132.88 bits (333), Expect = 1.299e-36
Identity = 85/235 (36.17%), Postives = 120/235 (51.06%), Query Frame = 1

Query:  118 NWDYLTYTLLWPQTFC----KYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCK-GDPFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECIKIEDDYYLSAVEICFDHQLKPINCPKHHHDNVDSQLKDLIHQIFQKVEGHLLLSDATKPINIPCNDNSFYIP 807
            +WDYL   + WP +FC    K  QC  D + VTGWN+HGLWPS+  G DP  CK    ++   L  I  DL+ +W  L   +   +FW HEW  HG CA    L   EL YF+ ++Q+K+ V L  IL  N+++P ++K Y        +     V PKL+C   +   Y+ A+E CFD +L  I CPK H      + +D + +   K        ++T PI+IPC     Y P
Sbjct:   25 DWDYLVMAVEWPPSFCYTFPKKIQC-CDTSHVTGWNLHGLWPSSSKGSDPLCCKNASAYDEKKLKPIMEDLLKEWNPLGNCSK--DFWKHEWKFHGTCASHNKLLHTELQYFNETIQMKKHVDLTNILHQNNVKPDNNKMYKVSDFVNVIKKVYKVEPKLQCTHYKGKIYILAMEFCFDTKLNIIMCPKRH------KSEDHLSRHRNKCRK---PRNSTNPIHIPCTTGFLYQP 247

[Close]

BLAST of S-like RNase vs. Planmine SMEST
Match: SMESG000048912.1 (SMESG000048912.1)

HSP 1 Score: 124.02 bits (310), Expect = 1.372e-33
Identity = 81/225 (36.00%), Postives = 114/225 (50.67%), Query Frame = 1

Query:  148 WPQTFC----KYNQCNYDKTKVTGWNIHGLWPSNITGKDPSKCK-GDPFNINDLNSIYPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDXXXXXXXXXXXXXXXXECIKIEDDYYLSAVEICFDHQLKPINCPKHHHDNVDSQLKDLIHQIFQKVEGHLLLSDATKPINIPCNDNSFYIP 807
            WP +FC    K  QC  D + VTGWN+HGLWPS+  G DP  CK    ++   L  I  DL+ +W  L   +   +FW HEW  HG CA    L   EL YF+ ++Q+K+ V L  IL  N+++P ++K Y        +     V PKL+C   +   Y+ A+E CFD +L  I CPK H      + +D + +   K        ++T PI+IPC     Y P
Sbjct:    5 WPPSFCYTFPKKIQC-CDTSHVTGWNLHGLWPSSSKGSDPLCCKNASAYDEKKLKPIMEDLLKEWNPLGNCS--KDFWKHEWKFHGTCASHNKLLHTELQYFNETIQMKKHVDLTNILHQNNVKPDNNKMYKVSDFVNVIKKVYKVEPKLQCTHYKGKIYILAMEFCFDTKLNIIMCPKRH------KSEDHLSRHRNKCRK---PRNSTNPIHIPCTTGFLYQP 217

The following BLAST results are available for this feature:

BLAST of S-like RNase vs. Ensembl Human
BLAST of S-like RNase vs. Ensembl Celegans
BLAST of S-like RNase vs. Ensembl Fly
BLAST of S-like RNase vs. Ensembl Zebrafish
BLAST of S-like RNase vs. Ensembl Xenopus
BLAST of S-like RNase vs. Ensembl Mouse
BLAST of S-like RNase vs. UniProt/SwissProt
BLAST of S-like RNase vs. TrEMBL
BLAST of S-like RNase vs. Ensembl Cavefish
BLAST of S-like RNase vs. Ensembl Sea Lamprey
BLAST of S-like RNase vs. Ensembl Yeast
BLAST of S-like RNase vs. Ensembl Nematostella
BLAST of S-like RNase vs. Ensembl Medaka
BLAST of S-like RNase vs. Planmine SMEST

BLAST of S-like RNase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
AL159163.1	1.144e-17	34.35	novel protein	[more]
RNASET2	2.527e-17	33.33	ribonuclease T2 [Source:HGNC Symbol;Acc:HGNC:21686...	[more]
RNASET2	2.527e-17	33.33	ribonuclease T2 [Source:HGNC Symbol;Acc:HGNC:21686...	[more]
RNASET2	2.711e-17	33.33	ribonuclease T2 [Source:HGNC Symbol;Acc:HGNC:21686...	[more]
RNASET2	1.186e-16	34.43	ribonuclease T2 [Source:HGNC Symbol;Acc:HGNC:21686...	[more]

BLAST of S-like RNase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
rnst-2	6.665e-21	29.19	pep chromosome:WBcel235:V:1061595:1067908:1 gene:W...	[more]

BLAST of S-like RNase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1


Match Name	E-value	Identity	Description
RNaseX25	8.856e-19	31.77	gene:FBgn0010406 transcript:FBtr0076752	[more]

BLAST of S-like RNase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
rnaset2	1.433e-22	33.77	ribonuclease T2 [Source:NCBI gene;Acc:791890]	[more]
rnaset2	1.433e-22	33.77	ribonuclease T2 [Source:NCBI gene;Acc:791890]	[more]
rnaset2	1.433e-22	33.77	ribonuclease T2 [Source:NCBI gene;Acc:791890]	[more]
rnaset2	1.433e-22	33.77	ribonuclease T2 [Source:NCBI gene;Acc:791890]	[more]
rnaset2	1.433e-22	33.77	ribonuclease T2 [Source:NCBI gene;Acc:791890]	[more]

BLAST of S-like RNase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1


Match Name	E-value	Identity	Description
rnaset2	3.579e-13	30.92	ribonuclease T2 [Source:NCBI gene;Acc:780173]	[more]

BLAST of S-like RNase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Rnaset2a	1.875e-15	32.58	ribonuclease T2A [Source:MGI Symbol;Acc:MGI:191544...	[more]
Rnaset2a	1.875e-15	32.58	ribonuclease T2A [Source:MGI Symbol;Acc:MGI:191544...	[more]
Gm49673	1.875e-15	32.58	predicted gene, 49673 [Source:MGI Symbol;Acc:MGI:6...	[more]
Gm49721	1.875e-15	32.58	predicted gene, 49721 [Source:MGI Symbol;Acc:MGI:6...	[more]
Rnaset2b	1.875e-15	32.58	ribonuclease T2B [Source:MGI Symbol;Acc:MGI:370208...	[more]

BLAST of S-like RNase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q7M456\|RNOY_CRAGI	6.647e-29	35.45	Ribonuclease Oy OS=Crassostrea gigas OX=29159 PE=1...	[more]
sp\|B8XY56\|RNT2_DANRE	1.082e-21	33.77	Ribonuclease T2 OS=Danio rerio OX=7955 GN=rnaset2 ...	[more]
sp\|O61887\|RNST2_CAEEL	8.478e-20	29.19	Ribonuclease T2 protein rnst-2 OS=Caenorhabditis e...	[more]
sp\|Q7M438\|RNDI_DICDI	3.738e-18	30.65	Ribonuclease DdI OS=Dictyostelium discoideum OX=44...	[more]
sp\|P42815\|RNS3_ARATH	3.390e-17	32.67	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=...	[more]

BLAST of S-like RNase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A4Z2DFD7	8.589e-29	36.98	Ribonuclease Oy isoform 2 OS=Schistosoma japonicum...	[more]
A0A4Z2DG04	1.283e-28	36.98	Ribonuclease Oy isoform 1 OS=Schistosoma japonicum...	[more]
G4V5B9	6.691e-28	35.24	Putative ribonuclease t2 OS=Schistosoma mansoni OX...	[more]
A0A4Z2DFD4	1.599e-27	36.36	Ribonuclease Oy OS=Schistosoma japonicum OX=6182 G...	[more]
Q5BVY2	2.692e-27	43.17	SJCHGC07916 protein (Fragment) OS=Schistosoma japo...	[more]

BLAST of S-like RNase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
rnaset2	1.259e-18	33.16	ribonuclease T2 [Source:NCBI gene;Acc:103036121]	[more]
rnaset2l	1.755e-15	29.17	ribonuclease Oy-like [Source:NCBI gene;Acc:1030229...	[more]

BLAST of S-like RNase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
rnaset2	9.600e-22	29.29	ribonuclease T2 [Source:NCBI gene;Acc:791890]	[more]

BLAST of S-like RNase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of S-like RNase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2


Match Name	E-value	Identity	Description
EDO48543	2.806e-24	31.31	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO48544	2.878e-18	31.82	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of S-like RNase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 4


Match Name	E-value	Identity	Description
rnaset2l	4.774e-20	35.46	ribonuclease Oy [Source:NCBI gene;Acc:101170531]	[more]
rnaset2l	4.774e-20	35.46	ribonuclease Oy [Source:NCBI gene;Acc:101170531]	[more]
rnaset2	2.998e-17	32.62	ribonuclease T2 [Source:NCBI gene;Acc:101163650]	[more]
rnaset2	2.998e-17	32.62	ribonuclease T2 [Source:NCBI gene;Acc:101163650]	[more]

BLAST of S-like RNase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000016389.1	5.930e-102	99.38	SMESG000016389.1	[more]
SMESG000046228.1	1.731e-37	36.24	SMESG000046228.1	[more]
SMESG000048912.1	5.462e-37	36.17	SMESG000048912.1	[more]
SMESG000048912.1	1.299e-36	36.17	SMESG000048912.1	[more]
SMESG000048912.1	1.372e-33	36.00	SMESG000048912.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30031930 ID=SMED30031930|Name=S-like RNase|organism=Schmidtea mediterranea sexual|type=transcript|length=1042bp
TAACAACAACAAGATTTTTAAGTATTTATAGAAAATTATTTTTATAATTT
GATTATTTGAAATATAATGTTAAAGCTTCTGATAATATTGTCGGTTGTGG
GTGTAAGTCTTTCAGCCAATTGGGATTATCTGACATATACTTTGCTATGG
CCTCAAACGTTTTGTAAATACAACCAGTGCAATTACGACAAGACAAAGGT
AACAGGATGGAATATTCATGGATTATGGCCATCTAATATAACAGGAAAGG
ATCCATCGAAATGCAAAGGCGATCCGTTCAATATTAATGATCTTAATAGT
ATTTATCCGGACCTGGTTGTGCAGTGGGCAAATTTATTAGAATTTACAAA
TCCTGCCGAATTCTGGACACATGAATGGACTAAGCACGGGTTGTGCGCAA
TTCTGCCGCCTTTATTTACAAATGAGCTTTCGTACTTTAGTCGCTCTCTA
CAATTGAAACAGTTTGTGTCTCTCGACAAAATTCTCTCCGACAACGACAT
CCAACCGAGCGACGATAAACAATATGACACGGATTCTGTCAGCAAATCAG
TATCGAGTAAGCTCGGAGTGTTACCGAAATTGGAATGCATTAAAATTGAA
GATGATTATTATTTGAGTGCAGTAGAAATATGTTTTGACCATCAACTGAA
GCCCATAAACTGTCCAAAGCATCACCATGATAATGTTGATAGCCAGCTTA
AAGACCTCATTCATCAAATATTCCAAAAGGTGGAAGGTCACCTACTACTC
TCCGATGCAACAAAGCCTATTAATATACCATGCAACGACAACTCATTTTA
CATTCCGGTTTAACTGTGCCAAACTTTACTAATATTTTATCACTTAAAAA
TTTTTATTCATAATTAATAGTAATTAAAAAAACCCATGTGACTCTTCAAA
TGTTCATTTTAAATCTGAATAATTATAAATGAGATTAGAAAGGTTATAGG
TCATCATGGTCAGTGATCACTTCATGAATGGAGATATTTCATTTTTTAAA
TTATATTTTTATTTATTAGAAAATATTATTTTAGTTAAAAAT

protein sequence of SMED30031930-orf-1

>SMED30031930-orf-1 ID=SMED30031930-orf-1|Name=SMED30031930-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=249bp
MLKLLIILSVVGVSLSANWDYLTYTLLWPQTFCKYNQCNYDKTKVTGWNI
HGLWPSNITGKDPSKCKGDPFNINDLNSIYPDLVVQWANLLEFTNPAEFW
THEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDD
KQYDTDSVSKSVSSKLGVLPKLECIKIEDDYYLSAVEICFDHQLKPINCP
KHHHDNVDSQLKDLIHQIFQKVEGHLLLSDATKPINIPCNDNSFYIPV*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000026	gut
PLANA:0000070	intestinal phagocyte
PLANA:0003116	parenchymal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR036430	RNase_T2-like_sf
IPR001568	RNase_T2-like
IPR018188	RNase_T2_His_AS_1
IPR033130	RNase_T2_His_AS_2

Vocabulary: **molecular function**
Term	Definition
GO:0003723	RNA binding
GO:0033897	ribonuclease T2 activity
GO:0004518	nuclease activity
GO:0004519	endonuclease activity
GO:0016787	hydrolase activity

Vocabulary: **cellular component**
Term	Definition
GO:0005576	extracellular region

Vocabulary: **biological process**
Term	Definition
GO:0090305	nucleic acid phosphodiester bond hydrolysis
GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036430	Ribonuclease T2-like superfamily	GENE3D	G3DSA:3.90.730.10		coord: 16..214 e-value: 1.8E-51 score: 176.8
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	SSF55895	Ribonuclease Rh-like	coord: 18..207
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 19..199 e-value: 1.3E-44 score: 152.6
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 5..208
None	No IPR available	PANTHER	PTHR11240:SF22	RIBONUCLEASE T2	coord: 5..208
None	No IPR available	SIGNALP_EUK	SignalP-noTM	SignalP-noTM	coord: 1..16 score: 0.878
IPR018188	Ribonuclease T2, His active site 1	PROSITE	PS00530	RNASE_T2_1	coord: 48..55
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 99..110

Main menu

S-like RNase

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 6

Owlery

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