calmodulin-like protein 3
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30031920 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Homology
BLAST of calmodulin-like protein 3 vs. TrEMBL
Match: A0A0B7BKS0 (Uncharacterized protein OS=Arion vulgaris OX=1028688 GN=ORF195836 PE=4 SV=1) HSP 1 Score: 166.777 bits (421), Expect = 1.098e-47 Identity = 76/152 (50.00%), Postives = 109/152 (71.71%), Query Frame = 3 Query: 384 SRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKGQAQR 839 +RKVP +++IFDKYV NNVR L+K +I+ML+ EF L + E MF TFD+D+NGK+S+WEFQQF C+G + Q+ + KF ELD D +G L+ EEARAG++ KT +G ++ KE++FF++ A ++G IDLG F LL RL++Y A + Sbjct: 5 NRKVPDHIMKIFDKYVANNVRVLAKQPAIDMLKTEFGLDDAEGEVMFNTFDKDKNGKLSLWEFQQFCVCMGEHAQEIVSKFKELDKDGSGQLDPEEARAGLHELKTATGRLLTDKEVDFFIQTAG-DDGSIDLGHFTNLLYRLKLYNAPAPK 155
BLAST of calmodulin-like protein 3 vs. TrEMBL
Match: A0A0B6Z0P6 (Uncharacterized protein OS=Arion vulgaris OX=1028688 GN=ORF44180 PE=4 SV=1) HSP 1 Score: 166.777 bits (421), Expect = 1.264e-47 Identity = 76/152 (50.00%), Postives = 109/152 (71.71%), Query Frame = 3 Query: 384 SRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKGQAQR 839 +RK+P ++++FDKYV NNVR L K +I+MLQ EF L E+A MF TFD+D+NGK+S+WEFQQF+ C+G + Q+ + KF ELD D +G L+ +EAR G+ KT +G ++ KE+EFF+ + A +NG IDLG+F LL RL++Y A + Sbjct: 5 NRKIPDNIVKMFDKYVANNVRILGKRSAIDMLQVEFGLDEEEASVMFTTFDKDKNGKLSIWEFQQFFICMGEHAQEIVTKFKELDQDGSGQLDPDEARVGLRQLKTATGRLLTDKEVEFFI-DTAGDNGVIDLGQFTNLLYRLKLYTAPAPK 155
BLAST of calmodulin-like protein 3 vs. TrEMBL
Match: A0A1S3K902 (calmodulin-like protein 3 OS=Lingula unguis OX=7574 GN=LOC106179854 PE=4 SV=1) HSP 1 Score: 166.392 bits (420), Expect = 2.939e-47 Identity = 74/150 (49.33%), Postives = 108/150 (72.00%), Query Frame = 3 Query: 378 MVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKG 827 M RK+PKEV +FDKY N+R L K+++I ML EF LT EQAE MF+TFD D NG +S+WEFQQFY VG++ + + KF E+D D +G ++ EEARAG T++G +++KE++FF+K ++E+G++D+GKF LL RL+ ++G Sbjct: 1 MPQRKLPKEVQAMFDKYTQKNIRRLKKEQAITMLTTEFGLTAEQAENMFKTFDSDGNGSLSMWEFQQFYTIVGSSVGELLAKFNEMDKDGSGAIDPEEARAGFKSLMTKTGRNLTEKEVDFFIKTTSSEDGQVDIGKFGHLLYRLKFFEG 150
BLAST of calmodulin-like protein 3 vs. TrEMBL
Match: R7V649 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_223283 PE=4 SV=1) HSP 1 Score: 160.614 bits (405), Expect = 2.512e-45 Identity = 78/149 (52.35%), Postives = 110/149 (73.83%), Query Frame = 3 Query: 378 MVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYK 824 MV+RK+PKEV IFD+YV NNVR L+KD++I ML+ EF L EQA MFE+FD D NG MS++EFQQFY CVG + Q+ ++KF ELD+D +G L++EEA+ G+ SG + +KEI+FF+ + ++G+ID+ +F LL RL+VY+ Sbjct: 1 MVARKIPKEVQAIFDRYVKNNVRRLAKDEAIAMLEKEFSLAPEQANAMFESFDGDHNGIMSLYEFQQFYQCVGNSAQEMVKKFEELDADGSGKLDVEEAKVGLQA----SG--LGEKEIDFFINSTKGKDGQIDIAQFGNLLFRLKVYE 143
BLAST of calmodulin-like protein 3 vs. TrEMBL
Match: K1QAD2 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10017744 PE=4 SV=1) HSP 1 Score: 157.918 bits (398), Expect = 5.370e-44 Identity = 77/152 (50.66%), Postives = 103/152 (67.76%), Query Frame = 3 Query: 378 MVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKGQA 833 M K+PKEV IFDKY NVR+L K +I +L+NEF L +A MF+TFD+DQN MS+WEFQQFY VG + + KF ELD D++G L+ +EA+ G+ T +G ++ +EIEFFLK A++E G ID+GKFV+L RL+VY A Sbjct: 2 MRRGKIPKEVQFIFDKYAHQNVRSLKKPDAINLLKNEFNLNDAEATCMFDTFDKDQNEIMSIWEFQQFYETVGNGAAEMLNKFHELDVDRSGKLDQQEAKEGLELMTTATGRKLDPREIEFFLKTASDEEGFIDIGKFVSLFKRLKVYDSPA 153
BLAST of calmodulin-like protein 3 vs. Ensembl Cavefish
Match: capns1a (calpain small subunit 1-like [Source:NCBI gene;Acc:103043763]) HSP 1 Score: 50.0618 bits (118), Expect = 6.242e-7 Identity = 41/160 (25.62%), Postives = 71/160 (44.38%), Query Frame = 3 Query: 336 RKFSFHFQSLVSSEM-VSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRL 812 R+F FQ L +M VS P E++ I +K +I+ +L D T E M D D GK+ EF+ + N +KW + + D+D++G + +E N FK +G ++ + + ++ ++E G +D + L RL Sbjct: 47 RQFRKVFQQLAGDDMEVS---PAELMNILNK-IISKHGDLKTDG----------FTIESCRSMVAVMDSDSTGKLGFHEFKHLW----NNIKKWQGIYKQFDADRSGVIGADELP---NSFKA-AGFPLNDQLFQLIIRRYSDEKGNMDFDNYTGCLVRL 184
BLAST of calmodulin-like protein 3 vs. Ensembl Nematostella
Match: EDO49093 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RI54]) HSP 1 Score: 48.1358 bits (113), Expect = 5.890e-7 Identity = 33/115 (28.70%), Postives = 51/115 (44.35%), Query Frame = 3 Query: 495 LTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKGQAQR 839 + E M D+D +GKM EF++ +A + +W F++ DSD++G + E +N F G+ +S + K A N I FVA RLR Q +R Sbjct: 39 FSIETCRLMITMLDRDYSGKMGFNEFKELWAAL----NQWKTTFMQYDSDRSGTMEPHELNNALNAF----GYRLSPACLAGITKRYAINNC-ISYDDFVACCVRLRALTDQFRR 144
BLAST of calmodulin-like protein 3 vs. Ensembl Medaka
Match: ENSORLT00000000601.2 (calpain small subunit 1 [Source:NCBI gene;Acc:101165329]) HSP 1 Score: 50.8322 bits (120), Expect = 3.638e-7 Identity = 39/160 (24.38%), Postives = 72/160 (45.00%), Query Frame = 3 Query: 336 RKFSFHFQSLVSSEM-VSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRL 812 ++F FQ L +M VS P E++ I +K VI+ ++L D + E + M D D G++ EF+ + N +KW ++ DSD +G + +E +G ++++ + ++ ++ENG +D F+ L RL Sbjct: 47 KQFRRVFQQLAGDDMEVS---PNELMDILNK-VISKRKDLKTDG----------FSIESCKSMVAVMDSDSTGRLGFHEFKHLWN----NIKKWQGIYLSYDSDHSGMIGSDELPRAFE----AAGFPLNEQLYKVIIRRYSDENGNMDFDNFIGCLVRL 184
BLAST of calmodulin-like protein 3 vs. Ensembl Medaka
Match: capns1b (calpain small subunit 1 [Source:NCBI gene;Acc:101165748]) HSP 1 Score: 46.9802 bits (110), Expect = 6.949e-6 Identity = 38/160 (23.75%), Postives = 70/160 (43.75%), Query Frame = 3 Query: 336 RKFSFHFQSLVSSEM-VSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRL 812 ++F F+ L +M VS P E++ I +K +I+ +L D + E M D D GK+ EF+ + N +KW + DSD +G ++ +E T +G ++ + + ++ ++ENG +D ++ L RL Sbjct: 47 KQFRRVFKQLAGDDMEVS---PPELMNILNK-IISKHGDLKTDG----------FSIESCRSMVAVMDSDSTGKLGFDEFKYLWN----NIKKWQAVYKTFDSDHSGLISADELPRAF----TAAGFPLNDQLFQMIIRRYSDENGNMDFDNYIGCLVRL 184
BLAST of calmodulin-like protein 3 vs. Planmine SMEST
Match: SMESG000067105.1 (SMESG000067105.1) HSP 1 Score: 375.941 bits (964), Expect = 2.018e-131 Identity = 204/208 (98.08%), Postives = 206/208 (99.04%), Query Frame = 3 Query: 216 YGQPTESTLKSAVQXXXXXXXXXXXXXXXXXXXQKCDINIRKFSFHFQSLVSSEMVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKGQAQR 839 Y + +ESTLKSAVQLCDSPCHHSSSLPSRRLHLQKCDINIRKFSFHFQSLVSSEMVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKGQAQR Sbjct: 88 YTRYSESTLKSAVQLCDSPCHHSSSLPSRRLHLQKCDINIRKFSFHFQSLVSSEMVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKGQAQR 295
BLAST of calmodulin-like protein 3 vs. Planmine SMEST
Match: SMESG000067105.1 (SMESG000067105.1) HSP 1 Score: 343.584 bits (880), Expect = 2.371e-118 Identity = 187/208 (89.90%), Postives = 197/208 (94.71%), Query Frame = 3 Query: 216 YGQPTESTLKSAVQXXXXXXXXXXXXXXXXXXXQKCDINIRKFSFHFQSLVSSEMVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKGQAQR 839 Y + +ESTLKSAVQLCDSPCHHSSSLPSRRLHLQKCDINIRKFSFHFQSLVSSEMVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGAN Q W+Q+FIELD DK+GHL+MEEARAGM+ FKTESGHE+S KEIEFFLKNAA+E G IDLGKFV LLSRL+VYKGQA R Sbjct: 102 YTRYSESTLKSAVQLCDSPCHHSSSLPSRRLHLQKCDINIRKFSFHFQSLVSSEMVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANAQAWVQRFIELDVDKSGHLDMEEARAGMSEFKTESGHEMSPKEIEFFLKNAADEKGLIDLGKFVGLLSRLKVYKGQAGR 309
BLAST of calmodulin-like protein 3 vs. Planmine SMEST
Match: SMESG000067105.1 (SMESG000067105.1) HSP 1 Score: 318.546 bits (815), Expect = 7.873e-111 Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 3 Query: 378 MVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKGQAQR 839 MVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKGQAQR Sbjct: 1 MVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKGQAQR 154
BLAST of calmodulin-like protein 3 vs. Planmine SMEST
Match: SMESG000067761.1 (SMESG000067761.1) HSP 1 Score: 48.1358 bits (113), Expect = 6.549e-6 Identity = 32/116 (27.59%), Postives = 50/116 (43.10%), Query Frame = 3 Query: 495 LTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLR----VYKGQ 830 + E M D DQ+G++ EF+ + + + W F + D D++G N E R +NH G +S + + AN+ G I ++ LL RLR YK Q Sbjct: 599 FSRETCRSMVAMMDTDQSGRLGFEEFKVLWD----DLRLWKTVFKKFDEDQSGTFNSYEIRQALNHI----GFRVSNRVFTAIITRYANKQGIITFDDYILLLVRLRTVFLTYKAQ 706 The following BLAST results are available for this feature:
BLAST of calmodulin-like protein 3 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of calmodulin-like protein 3 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of calmodulin-like protein 3 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of calmodulin-like protein 3 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of calmodulin-like protein 3 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of calmodulin-like protein 3 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of calmodulin-like protein 3 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of calmodulin-like protein 3 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of calmodulin-like protein 3 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 1
BLAST of calmodulin-like protein 3 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of calmodulin-like protein 3 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of calmodulin-like protein 3 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of calmodulin-like protein 3 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 2
BLAST of calmodulin-like protein 3 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 4
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30031920 ID=SMED30031920|Name=calmodulin-like protein 3|organism=Schmidtea mediterranea sexual|type=transcript|length=945bpback to top protein sequence of SMED30031920-orf-1 >SMED30031920-orf-1 ID=SMED30031920-orf-1|Name=SMED30031920-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=155bp MVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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