calmodulin-like protein 3

Overview
Namecalmodulin-like protein 3
Smed IDSMED30031920
Length (bp)945
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of calmodulin-like protein 3 (SMED30031920) t-SNE clustered cells

Violin plots show distribution of expression levels for calmodulin-like protein 3 (SMED30031920) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of calmodulin-like protein 3 (SMED30031920) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for calmodulin-like protein 3 (SMED30031920) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30031920

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X2 cellSMED30031920SMESG000067105.1 SmedASXL_009407SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
cephalic gangliaSMED30031920SMESG000067105.1 dd_Smed_v4_4944_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30031920SMESG000067105.1 dd_Smed_v4_4944_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30031920SMESG000067105.1 dd_Smed_v4_4944_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031920SMESG000067105.1 dd_Smed_v4_4944_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30031920SMESG000067105.1 Contig33951newmark_estsPMID:27612384
Roberts-Galbraith et al., 2016
whole organism asexual adult colorimetric in situ hybridization evidence
neuronSMED30031920SMESG000067105.1 Contig33951uc_Smed_v2PMID:27612384
Roberts-Galbraith et al., 2016
whole organism asexual adult colorimetric in situ hybridization evidence
enteric muscle cellSMED30031920SMESG000067105.1 dd_Smed_v4_4944_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of calmodulin-like protein 3 vs. TrEMBL
Match: A0A0B7BKS0 (Uncharacterized protein OS=Arion vulgaris OX=1028688 GN=ORF195836 PE=4 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 1.098e-47
Identity = 76/152 (50.00%), Postives = 109/152 (71.71%), Query Frame = 3
Query:  384 SRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKGQAQR 839
            +RKVP  +++IFDKYV NNVR L+K  +I+ML+ EF L   + E MF TFD+D+NGK+S+WEFQQF  C+G + Q+ + KF ELD D +G L+ EEARAG++  KT +G  ++ KE++FF++ A  ++G IDLG F  LL RL++Y   A +
Sbjct:    5 NRKVPDHIMKIFDKYVANNVRVLAKQPAIDMLKTEFGLDDAEGEVMFNTFDKDKNGKLSLWEFQQFCVCMGEHAQEIVSKFKELDKDGSGQLDPEEARAGLHELKTATGRLLTDKEVDFFIQTAG-DDGSIDLGHFTNLLYRLKLYNAPAPK 155          
BLAST of calmodulin-like protein 3 vs. TrEMBL
Match: A0A0B6Z0P6 (Uncharacterized protein OS=Arion vulgaris OX=1028688 GN=ORF44180 PE=4 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 1.264e-47
Identity = 76/152 (50.00%), Postives = 109/152 (71.71%), Query Frame = 3
Query:  384 SRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKGQAQR 839
            +RK+P  ++++FDKYV NNVR L K  +I+MLQ EF L  E+A  MF TFD+D+NGK+S+WEFQQF+ C+G + Q+ + KF ELD D +G L+ +EAR G+   KT +G  ++ KE+EFF+ + A +NG IDLG+F  LL RL++Y   A +
Sbjct:    5 NRKIPDNIVKMFDKYVANNVRILGKRSAIDMLQVEFGLDEEEASVMFTTFDKDKNGKLSIWEFQQFFICMGEHAQEIVTKFKELDQDGSGQLDPDEARVGLRQLKTATGRLLTDKEVEFFI-DTAGDNGVIDLGQFTNLLYRLKLYTAPAPK 155          
BLAST of calmodulin-like protein 3 vs. TrEMBL
Match: A0A1S3K902 (calmodulin-like protein 3 OS=Lingula unguis OX=7574 GN=LOC106179854 PE=4 SV=1)

HSP 1 Score: 166.392 bits (420), Expect = 2.939e-47
Identity = 74/150 (49.33%), Postives = 108/150 (72.00%), Query Frame = 3
Query:  378 MVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKG 827
            M  RK+PKEV  +FDKY   N+R L K+++I ML  EF LT EQAE MF+TFD D NG +S+WEFQQFY  VG++  + + KF E+D D +G ++ EEARAG     T++G  +++KE++FF+K  ++E+G++D+GKF  LL RL+ ++G
Sbjct:    1 MPQRKLPKEVQAMFDKYTQKNIRRLKKEQAITMLTTEFGLTAEQAENMFKTFDSDGNGSLSMWEFQQFYTIVGSSVGELLAKFNEMDKDGSGAIDPEEARAGFKSLMTKTGRNLTEKEVDFFIKTTSSEDGQVDIGKFGHLLYRLKFFEG 150          
BLAST of calmodulin-like protein 3 vs. TrEMBL
Match: R7V649 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_223283 PE=4 SV=1)

HSP 1 Score: 160.614 bits (405), Expect = 2.512e-45
Identity = 78/149 (52.35%), Postives = 110/149 (73.83%), Query Frame = 3
Query:  378 MVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYK 824
            MV+RK+PKEV  IFD+YV NNVR L+KD++I ML+ EF L  EQA  MFE+FD D NG MS++EFQQFY CVG + Q+ ++KF ELD+D +G L++EEA+ G+      SG  + +KEI+FF+ +   ++G+ID+ +F  LL RL+VY+
Sbjct:    1 MVARKIPKEVQAIFDRYVKNNVRRLAKDEAIAMLEKEFSLAPEQANAMFESFDGDHNGIMSLYEFQQFYQCVGNSAQEMVKKFEELDADGSGKLDVEEAKVGLQA----SG--LGEKEIDFFINSTKGKDGQIDIAQFGNLLFRLKVYE 143          
BLAST of calmodulin-like protein 3 vs. TrEMBL
Match: K1QAD2 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10017744 PE=4 SV=1)

HSP 1 Score: 157.918 bits (398), Expect = 5.370e-44
Identity = 77/152 (50.66%), Postives = 103/152 (67.76%), Query Frame = 3
Query:  378 MVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKGQA 833
            M   K+PKEV  IFDKY   NVR+L K  +I +L+NEF L   +A  MF+TFD+DQN  MS+WEFQQFY  VG    + + KF ELD D++G L+ +EA+ G+    T +G ++  +EIEFFLK A++E G ID+GKFV+L  RL+VY   A
Sbjct:    2 MRRGKIPKEVQFIFDKYAHQNVRSLKKPDAINLLKNEFNLNDAEATCMFDTFDKDQNEIMSIWEFQQFYETVGNGAAEMLNKFHELDVDRSGKLDQQEAKEGLELMTTATGRKLDPREIEFFLKTASDEEGFIDIGKFVSLFKRLKVYDSPA 153          
BLAST of calmodulin-like protein 3 vs. Ensembl Cavefish
Match: capns1a (calpain small subunit 1-like [Source:NCBI gene;Acc:103043763])

HSP 1 Score: 50.0618 bits (118), Expect = 6.242e-7
Identity = 41/160 (25.62%), Postives = 71/160 (44.38%), Query Frame = 3
Query:  336 RKFSFHFQSLVSSEM-VSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRL 812
            R+F   FQ L   +M VS   P E++ I +K +I+   +L  D            T E    M    D D  GK+   EF+  +     N +KW   + + D+D++G +  +E     N FK  +G  ++ +  +  ++  ++E G +D   +   L RL
Sbjct:   47 RQFRKVFQQLAGDDMEVS---PAELMNILNK-IISKHGDLKTDG----------FTIESCRSMVAVMDSDSTGKLGFHEFKHLW----NNIKKWQGIYKQFDADRSGVIGADELP---NSFKA-AGFPLNDQLFQLIIRRYSDEKGNMDFDNYTGCLVRL 184          
BLAST of calmodulin-like protein 3 vs. Ensembl Nematostella
Match: EDO49093 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RI54])

HSP 1 Score: 48.1358 bits (113), Expect = 5.890e-7
Identity = 33/115 (28.70%), Postives = 51/115 (44.35%), Query Frame = 3
Query:  495 LTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKGQAQR 839
             + E    M    D+D +GKM   EF++ +A +     +W   F++ DSD++G +   E    +N F    G+ +S   +    K  A  N  I    FVA   RLR    Q +R
Sbjct:   39 FSIETCRLMITMLDRDYSGKMGFNEFKELWAAL----NQWKTTFMQYDSDRSGTMEPHELNNALNAF----GYRLSPACLAGITKRYAINNC-ISYDDFVACCVRLRALTDQFRR 144          
BLAST of calmodulin-like protein 3 vs. Ensembl Medaka
Match: ENSORLT00000000601.2 (calpain small subunit 1 [Source:NCBI gene;Acc:101165329])

HSP 1 Score: 50.8322 bits (120), Expect = 3.638e-7
Identity = 39/160 (24.38%), Postives = 72/160 (45.00%), Query Frame = 3
Query:  336 RKFSFHFQSLVSSEM-VSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRL 812
            ++F   FQ L   +M VS   P E++ I +K VI+  ++L  D            + E  + M    D D  G++   EF+  +     N +KW   ++  DSD +G +  +E           +G  ++++  +  ++  ++ENG +D   F+  L RL
Sbjct:   47 KQFRRVFQQLAGDDMEVS---PNELMDILNK-VISKRKDLKTDG----------FSIESCKSMVAVMDSDSTGRLGFHEFKHLWN----NIKKWQGIYLSYDSDHSGMIGSDELPRAFE----AAGFPLNEQLYKVIIRRYSDENGNMDFDNFIGCLVRL 184          
BLAST of calmodulin-like protein 3 vs. Ensembl Medaka
Match: capns1b (calpain small subunit 1 [Source:NCBI gene;Acc:101165748])

HSP 1 Score: 46.9802 bits (110), Expect = 6.949e-6
Identity = 38/160 (23.75%), Postives = 70/160 (43.75%), Query Frame = 3
Query:  336 RKFSFHFQSLVSSEM-VSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRL 812
            ++F   F+ L   +M VS   P E++ I +K +I+   +L  D            + E    M    D D  GK+   EF+  +     N +KW   +   DSD +G ++ +E         T +G  ++ +  +  ++  ++ENG +D   ++  L RL
Sbjct:   47 KQFRRVFKQLAGDDMEVS---PPELMNILNK-IISKHGDLKTDG----------FSIESCRSMVAVMDSDSTGKLGFDEFKYLWN----NIKKWQAVYKTFDSDHSGLISADELPRAF----TAAGFPLNDQLFQMIIRRYSDENGNMDFDNYIGCLVRL 184          
BLAST of calmodulin-like protein 3 vs. Planmine SMEST
Match: SMESG000067105.1 (SMESG000067105.1)

HSP 1 Score: 375.941 bits (964), Expect = 2.018e-131
Identity = 204/208 (98.08%), Postives = 206/208 (99.04%), Query Frame = 3
Query:  216 YGQPTESTLKSAVQXXXXXXXXXXXXXXXXXXXQKCDINIRKFSFHFQSLVSSEMVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKGQAQR 839
            Y + +ESTLKSAVQLCDSPCHHSSSLPSRRLHLQKCDINIRKFSFHFQSLVSSEMVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKGQAQR
Sbjct:   88 YTRYSESTLKSAVQLCDSPCHHSSSLPSRRLHLQKCDINIRKFSFHFQSLVSSEMVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKGQAQR 295          
BLAST of calmodulin-like protein 3 vs. Planmine SMEST
Match: SMESG000067105.1 (SMESG000067105.1)

HSP 1 Score: 343.584 bits (880), Expect = 2.371e-118
Identity = 187/208 (89.90%), Postives = 197/208 (94.71%), Query Frame = 3
Query:  216 YGQPTESTLKSAVQXXXXXXXXXXXXXXXXXXXQKCDINIRKFSFHFQSLVSSEMVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKGQAQR 839
            Y + +ESTLKSAVQLCDSPCHHSSSLPSRRLHLQKCDINIRKFSFHFQSLVSSEMVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGAN Q W+Q+FIELD DK+GHL+MEEARAGM+ FKTESGHE+S KEIEFFLKNAA+E G IDLGKFV LLSRL+VYKGQA R
Sbjct:  102 YTRYSESTLKSAVQLCDSPCHHSSSLPSRRLHLQKCDINIRKFSFHFQSLVSSEMVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANAQAWVQRFIELDVDKSGHLDMEEARAGMSEFKTESGHEMSPKEIEFFLKNAADEKGLIDLGKFVGLLSRLKVYKGQAGR 309          
BLAST of calmodulin-like protein 3 vs. Planmine SMEST
Match: SMESG000067105.1 (SMESG000067105.1)

HSP 1 Score: 318.546 bits (815), Expect = 7.873e-111
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 3
Query:  378 MVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKGQAQR 839
            MVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKGQAQR
Sbjct:    1 MVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKGQAQR 154          
BLAST of calmodulin-like protein 3 vs. Planmine SMEST
Match: SMESG000067761.1 (SMESG000067761.1)

HSP 1 Score: 48.1358 bits (113), Expect = 6.549e-6
Identity = 32/116 (27.59%), Postives = 50/116 (43.10%), Query Frame = 3
Query:  495 LTTEQAEQMFETFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEARAGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLR----VYKGQ 830
             + E    M    D DQ+G++   EF+  +     + + W   F + D D++G  N  E R  +NH     G  +S +     +   AN+ G I    ++ LL RLR     YK Q
Sbjct:  599 FSRETCRSMVAMMDTDQSGRLGFEEFKVLWD----DLRLWKTVFKKFDEDQSGTFNSYEIRQALNHI----GFRVSNRVFTAIITRYANKQGIITFDDYILLLVRLRTVFLTYKAQ 706          
The following BLAST results are available for this feature:
BLAST of calmodulin-like protein 3 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of calmodulin-like protein 3 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of calmodulin-like protein 3 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of calmodulin-like protein 3 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of calmodulin-like protein 3 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of calmodulin-like protein 3 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of calmodulin-like protein 3 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of calmodulin-like protein 3 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0B7BKS01.098e-4750.00Uncharacterized protein OS=Arion vulgaris OX=10286... [more]
A0A0B6Z0P61.264e-4750.00Uncharacterized protein OS=Arion vulgaris OX=10286... [more]
A0A1S3K9022.939e-4749.33calmodulin-like protein 3 OS=Lingula unguis OX=757... [more]
R7V6492.512e-4552.35Uncharacterized protein OS=Capitella teleta OX=283... [more]
K1QAD25.370e-4450.66Uncharacterized protein OS=Crassostrea gigas OX=29... [more]
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BLAST of calmodulin-like protein 3 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
capns1a6.242e-725.63calpain small subunit 1-like [Source:NCBI gene;Acc... [more]
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BLAST of calmodulin-like protein 3 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of calmodulin-like protein 3 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of calmodulin-like protein 3 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO490935.890e-728.70Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of calmodulin-like protein 3 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSORLT00000000601.23.638e-724.38calpain small subunit 1 [Source:NCBI gene;Acc:1011... [more]
capns1b6.949e-623.75calpain small subunit 1 [Source:NCBI gene;Acc:1011... [more]
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BLAST of calmodulin-like protein 3 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000067105.12.018e-13198.08SMESG000067105.1[more]
SMESG000067105.12.371e-11889.90SMESG000067105.1[more]
SMESG000067105.17.873e-111100.00SMESG000067105.1[more]
SMESG000067761.16.549e-627.59SMESG000067761.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031920 ID=SMED30031920|Name=calmodulin-like protein 3|organism=Schmidtea mediterranea sexual|type=transcript|length=945bp
CTTAGTCACATCGGATGAAAAATTTTCCCAAAAATCGTAACATACATTTT
GGCGATTTTATTTTGTCCTTTTTATTCAGACAGTAAATTATGTTTCATGC
CAACTGAAAAATACAAATAAAACAGAAAAATATCATTTAATAAGCAGACA
AGTGTTTCGTCATATTTTCAATCTTGATCTTTTCTGGTGATTAAATCATT
TTCTCTAATGTGAAATACGGTCAGCCCACAGAAAGCACACTGAAAAGTGC
TGTGCAATTATGCGATTCTCCTTGCCACCATTCATCAAGTCTTCCTTCTC
GCCGATTACACCTACAAAAATGCGATATAAATATTCGAAAATTTTCATTT
CATTTTCAGTCTCTCGTATCAAGCGAAATGGTCAGCAGAAAAGTGCCTAA
AGAAGTTCTTCAGATATTCGATAAATATGTCATAAATAATGTTCGAAATT
TGTCAAAGGACAAATCCATTGAAATGCTACAAAATGAATTTCAATTGACT
ACTGAACAAGCAGAGCAGATGTTTGAAACATTTGATCAAGATCAAAACGG
AAAAATGAGCGTTTGGGAATTTCAACAGTTTTATGCCTGCGTTGGAGCAA
ATACACAAAAATGGATCCAGAAATTTATCGAGCTCGACTCGGACAAAAAC
GGCCATTTAAACATGGAAGAAGCAAGAGCGGGAATGAATCACTTTAAAAC
CGAATCCGGTCATGAAATTTCACAGAAAGAAATTGAATTCTTTCTCAAGA
ACGCTGCAAACGAAAACGGGGAAATCGATTTGGGGAAATTTGTGGCGCTT
CTTTCAAGACTGAGGGTGTACAAGGGTCAAGCTCAACGCTAATTTATTTC
CATTTCTTTTACAATTTTTTATGGACACATTGCGCTATTTATGATTTTAT
ACGATTTATGAAACTTTTAGCTAAATAAATGTTGAAATTTAAAAA
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protein sequence of SMED30031920-orf-1

>SMED30031920-orf-1 ID=SMED30031920-orf-1|Name=SMED30031920-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=155bp
MVSRKVPKEVLQIFDKYVINNVRNLSKDKSIEMLQNEFQLTTEQAEQMFE
TFDQDQNGKMSVWEFQQFYACVGANTQKWIQKFIELDSDKNGHLNMEEAR
AGMNHFKTESGHEISQKEIEFFLKNAANENGEIDLGKFVALLSRLRVYKG
QAQR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0002111X2 cell
PLANA:0003104enteric muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 78..106
e-value: 5.0
score: 12.1
coord: 44..72
e-value: 0.59
score: 19.2
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 40..75
score: 12.282
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 77..109
score: 8.209
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 3..153
e-value: 9.1E-23
score: 82.7
NoneNo IPR availablePANTHERPTHR46212:SF3PROGRAMMED CELL DEATH 6coord: 9..146
NoneNo IPR availablePANTHERPTHR46212FAMILY NOT NAMEDcoord: 9..146
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 53..65
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 8..146