SJCHGC09680 protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30031906 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Homology
BLAST of SJCHGC09680 protein vs. TrEMBL
Match: A0A4Z2D1V3 (Uncharacterized protein OS=Schistosoma japonicum OX=6182 GN=EWB00_005417 PE=4 SV=1) HSP 1 Score: 291.197 bits (744), Expect = 1.644e-91 Identity = 147/316 (46.52%), Postives = 197/316 (62.34%), Query Frame = 1 Query: 241 QVVLINKTIKGKFYCPEPGQSKSLYHCCGHIGEQYCCDPQDTPGFKWGIAVAVTVGVLVILALGYTIQLFRACDKCYHECPICSVSNNDEEFQWETRWYSVSHEGNRYGVTFHSHKFAKYFMQKLELQYGPWNTANILRIKREDHDFVVLNIFFHDLFTPWFYTQGPIKNDEEWIKSDFSQMCNMVKEEIAVYP-----CRCCPFSNMKKASERTKF------------------DTAFHNNNNRPFNKYLKPWTKSTF-----RVSNSQSLSINKPIAATKT-----------VIVLEDIIEVYGNSKAIQTKRE 1071 +++L+N GKFYCP+P Q++ + +CCG +G+Q CC DTPG +WGI + V V ++V L + YTIQ+FRACD+CYHECPI + + +D EFQW+TRWY+VSHEGNRYGVTFHS FA FM+ LE +YG WNTANIL+I+R+ +FV+LNIFFHDLFTPWFYTQGPIKNDEEWI++DF +C+ V+ +AV P C + K S++T +T+ H N+ PWTK + S + L+ N KT VI+LEDIIE G K +QT+++ Sbjct: 118 KLILLNS---GKFYCPQPNQTEDMKYCCGPLGKQTCCRITDTPGLRWGIPLGVIVSIIVFLTVSYTIQVFRACDRCYHECPIFTPTPSDAEFQWDTRWYTVSHEGNRYGVTFHSRLFANEFMKCLEAEYGRWNTANILKIQRDGQNFVILNIFFHDLFTPWFYTQGPIKNDEEWIRTDFKNICHTVQARLAVNPRFKWKLNKCFKHSTNKTSKQTNHSLINLNSPELINSHLDNKNTSHHLTNSNTVTTPWIPWTKVAYIPSLTETSVKKHLTRNTNKIKNKTKNDNKTNQLVNVIILEDIIECNGLDKGVQTQQK 430
BLAST of SJCHGC09680 protein vs. TrEMBL
Match: A0A5K4F5A6 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 287.73 bits (735), Expect = 2.817e-90 Identity = 147/310 (47.42%), Postives = 193/310 (62.26%), Query Frame = 1 Query: 241 QVVLINKTIKGKFYCPEPGQSKSLYHCCGHIGEQYCCDPQDTPGFKWGIAVAVTVGVLVILALGYTIQLFRACDKCYHECPICSVSNNDEEFQWETRWYSVSHEGNRYGVTFHSHKFAKYFMQKLELQYGPWNTANILRIKREDHDFVVLNIFFHDLFTPWFYTQGPIKNDEEWIKSDFSQMCNMVKEEIAVYP---CRCCPFSN---------MKKASERTKFDTAFHNNNNRPFN--------------KYLKPWTKSTF-----RVSNSQSLSINKPIAATK--TVIVLEDIIEVYGNSKAIQTKRE 1071 +++LIN GKFYCP+P QS+ + +CCG +G+Q CC DTPG +WGI + V V V+V L + YTIQ+FRACD+CYHECPI + + ND EFQW+TRWY+VSHEGNRYGVTFHS FA FM+ LE +YG WNTANIL+I+R+ +FV+LNIFFHDLFTPWFYTQGPIKNDEEWI++DF +C+ V+ +AV P + F N ++S T N +R N Y+ T+++ R SN + + VI+LEDIIE G K +QT ++ Sbjct: 113 KIILINS---GKFYCPQPNQSEDMKYCCGPLGKQICCKITDTPGLRWGIPLGVIVTVIVFLTVSYTIQVFRACDRCYHECPIFTPTPNDAEFQWDTRWYTVSHEGNRYGVTFHSRIFANEFMKCLEAEYGRWNTANILKIQRDGQNFVILNIFFHDLFTPWFYTQGPIKNDEEWIRTDFKNICHTVQARLAVNPRFKWKLNKFPNNSSNKTLRQTNQSSNNTNISDLDQKNIDRLSNPNTVTTPWTPWTKVAYIPSLTETSVKKNLTRNSNKSKYKMKNEYKPNQLVNVIILEDIIECNGLDKGVQTHQK 419
BLAST of SJCHGC09680 protein vs. TrEMBL
Match: A0A4E0RGL4 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_003055 PE=4 SV=1) HSP 1 Score: 284.648 bits (727), Expect = 2.151e-89 Identity = 147/322 (45.65%), Postives = 197/322 (61.18%), Query Frame = 1 Query: 238 NQVVLINKTIKGKFYCPEPGQSKSLYHCCGHIGEQYCCDPQDTPGFKWGIAVAVTVGVLVILALGYTIQLFRACDKCYHECPICSVSNNDEEFQWETRWYSVSHEGNRYGVTFHSHKFAKYFMQKLELQYGPWNTANILRIKREDHDFVVLNIFFHDLFTPWFYTQGPIKNDEEWIKSDFSQMCNMVKEEIAVYPCRC---------------CP----------FSNMKKASERTKFDTAFHNNNNRPFNKYLKPWTKSTF-------RVSNSQSL----SINKPIAATK--------TVIVLEDIIEVYGNSKAIQTKRE 1071 QV L+ K GKF CPE + + +CCG +G QYCC DTPG KWG+AV V V +V L + YTIQ FR CD+CYHECPI ++ + +EFQ +TRWY+VSHEGNRYGVTFHS FAK FM+ LE +YG WNTANIL+I+R+ DFV+LNIFFHD+FTPWFYTQGPIKNDEEWI++DF MC V+ ++AV C C CP S S+ T+ ++ + N+ +K++ PWTK+++ N++SL + K + T VI+ E++IE G K +QT+++ Sbjct: 74 GQVELLLKR-SGKFQCPEQNHTNDMTYCCGELGAQYCCKLTDTPGLKWGVAVGVIVTFIVFLTVSYTIQFFRVCDRCYHECPIVGINPDLQEFQMDTRWYTVSHEGNRYGVTFHSRSFAKEFMECLEEKYGRWNTANILKIQRDGQDFVILNIFFHDVFTPWFYTQGPIKNDEEWIRTDFRAMCQAVRSKLAVRICLCLRLKQNHLSSSGSQQCPDNIQLESVVDTSAEIAKSDNTQQTSSAEKSCNKVMHKWV-PWTKASYTPPAFQDEAPNTKSLKRCSTRTKSMRKTDRNEAQSIVNVIIFEELIECNGLDKGVQTRQK 393
BLAST of SJCHGC09680 protein vs. TrEMBL
Match: A0A3R7D7L3 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CSKR_495s PE=4 SV=1) HSP 1 Score: 273.092 bits (697), Expect = 7.124e-85 Identity = 131/272 (48.16%), Postives = 165/272 (60.66%), Query Frame = 1 Query: 217 VELMTCVNQVVLINKTIKGKFYCPEPGQSKSLYHCCGHIGEQYCCDPQDTPGFKWGIAVAVTVGVLVILALGYTIQLFRACDKCYHECPICSVSNNDEEFQWETRWYSVSHEGNRYGVTFHSHKFAKYFMQKLELQYGPWNTANILRIKREDHDFVVLNIFFHDLFTPWFYTQGPIKNDEEWIKSDFSQMCNMVKEEIAVY----PCRCCPFSNMKKASERTKFDTAFHNNNNRPFNKYLK----------PWTKSTFRVSNS-QSLSINKP 987 +E+ +Q + GKF CP P ++ + +CCG + EQ+CC QDTPG KWG+AV V V ++V L + YTIQ+FR CD+CYHECPI ++ EFQ TRWY+VSHEGNRYGVTFHS FA FMQ+LE +YG WNTANIL+I+R+ DFV+L+IFFHDLFTPWFYTQGPIKNDEEWI++DF +C VK + V R C N D N +K + PWTK+ + S QS P Sbjct: 73 IEIGQLGSQQTELGLIQSGKFACPRPNETSDMVYCCGPVREQHCCRLQDTPGLKWGVAVGVVVTLIVFLTVSYTIQVFRVCDRCYHECPIVGFGADETEFQLNTRWYTVSHEGNRYGVTFHSRGFANQFMQRLEERYGRWNTANILKIQRDGQDFVILSIFFHDLFTPWFYTQGPIKNDEEWIRTDFKSICQTVKANLRVTLWGPSWRSCSTENDPNEGNLRNEDLQLGTNKPESSDKLGRVPSRLARRWVPWTKAIYLPPTSNQSTGTESP 344
BLAST of SJCHGC09680 protein vs. TrEMBL
Match: A0A4S2MCQ1 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_002371 PE=4 SV=1) HSP 1 Score: 272.322 bits (695), Expect = 1.960e-84 Identity = 117/200 (58.50%), Postives = 148/200 (74.00%), Query Frame = 1 Query: 217 VELMTCVNQVVLINKTIKGKFYCPEPGQSKSLYHCCGHIGEQYCCDPQDTPGFKWGIAVAVTVGVLVILALGYTIQLFRACDKCYHECPICSVSNNDEEFQWETRWYSVSHEGNRYGVTFHSHKFAKYFMQKLELQYGPWNTANILRIKREDHDFVVLNIFFHDLFTPWFYTQGPIKNDEEWIKSDFSQMCNMVKEEIAV 816 +E+ +Q + + GKF CP P ++ + +CCG + EQ+CC QDTPG KWG+AV V V ++V L + YTIQ+FR CD+CYHECPI ++ EFQ TRWY+VSHEGNRYGVTFHS FA FMQ+LE +YG WNTANIL+I+R+ DFV+L+IFFHDLFTPWFYTQGPIKNDEEWI++DF +C VK + V Sbjct: 84 IEIGQFGSQQIELGLIQSGKFVCPRPNETSEMIYCCGPVREQHCCRLQDTPGLKWGVAVGVVVTLIVFLTVSYTIQVFRVCDRCYHECPIIGFGEDETEFQLNTRWYTVSHEGNRYGVTFHSRGFANQFMQRLEERYGRWNTANILKIQRDGQDFVILSIFFHDLFTPWFYTQGPIKNDEEWIRTDFKSICQTVKANLRV 283
BLAST of SJCHGC09680 protein vs. Planmine SMEST
Match: SMESG000000717.1 (SMESG000000717.1) HSP 1 Score: 486.108 bits (1250), Expect = 8.468e-175 Identity = 229/229 (100.00%), Postives = 229/229 (100.00%), Query Frame = 1 Query: 115 MPNLFSKKFLWKYLIFTLFYYQYQFTRIKQSLEEVELMTCVNQVVLINKTIKGKFYCPEPGQSKSLYHCCGHIGEQYCCDPQDTPGFKWGIAVAVTVGVLVILALGYTIQLFRACDKCYHECPICSVSNNDEEFQWETRWYSVSHEGNRYGVTFHSHKFAKYFMQKLELQYGPWNTANILRIKREDHDFVVLNIFFHDLFTPWFYTQGPIKNDEEWIKSDFSQMCNMVK 801 MPNLFSKKFLWKYLIFTLFYYQYQFTRIKQSLEEVELMTCVNQVVLINKTIKGKFYCPEPGQSKSLYHCCGHIGEQYCCDPQDTPGFKWGIAVAVTVGVLVILALGYTIQLFRACDKCYHECPICSVSNNDEEFQWETRWYSVSHEGNRYGVTFHSHKFAKYFMQKLELQYGPWNTANILRIKREDHDFVVLNIFFHDLFTPWFYTQGPIKNDEEWIKSDFSQMCNMVK Sbjct: 1 MPNLFSKKFLWKYLIFTLFYYQYQFTRIKQSLEEVELMTCVNQVVLINKTIKGKFYCPEPGQSKSLYHCCGHIGEQYCCDPQDTPGFKWGIAVAVTVGVLVILALGYTIQLFRACDKCYHECPICSVSNNDEEFQWETRWYSVSHEGNRYGVTFHSHKFAKYFMQKLELQYGPWNTANILRIKREDHDFVVLNIFFHDLFTPWFYTQGPIKNDEEWIKSDFSQMCNMVK 229 The following BLAST results are available for this feature:
BLAST of SJCHGC09680 protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SJCHGC09680 protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SJCHGC09680 protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SJCHGC09680 protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SJCHGC09680 protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SJCHGC09680 protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SJCHGC09680 protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SJCHGC09680 protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SJCHGC09680 protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SJCHGC09680 protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SJCHGC09680 protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SJCHGC09680 protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SJCHGC09680 protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SJCHGC09680 protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30031906 ID=SMED30031906|Name=SJCHGC09680 protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1124bpback to top protein sequence of SMED30031906-orf-1 >SMED30031906-orf-1 ID=SMED30031906-orf-1|Name=SMED30031906-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=323bp MPNLFSKKFLWKYLIFTLFYYQYQFTRIKQSLEEVELMTCVNQVVLINKTback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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