SMED30031905

Overview
NameSMED30031905
Smed IDSMED30031905
Length (bp)2783
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30031905 (SMED30031905) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30031905 (SMED30031905) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30031905 (SMED30031905) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30031905 (SMED30031905) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30031905

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30031905h1SMcG0012261 PL08010A1A04smed_ncbi_20240424PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
posterior sideSMED30031905h1SMcG0012261 dd_Smed_v4_6343_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30031905h1SMcG0012261 dd_Smed_v4_6343_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30031905 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.005618:10040..30791 -11885
Homology
BLAST of SMED30031905 vs. Planmine SMEST
Match: SMESG000004351.1 (SMESG000004351.1)

HSP 1 Score: 1615.51 bits (4182), Expect = 0.000e+0
Identity = 858/866 (99.08%), Postives = 861/866 (99.42%), Query Frame = 2
Query:  113 MNANDYNAAEIMIEEHQIIITNDILKDAHFINDNHKNKLVKIKKTKFTGVVEHVFTLYDDKLVAVGNIYPQQNDISFQDLLLDNSEFRGFIIKDSRVKVLDSTIKFVEDSKLIDQNTIYHINFKAIEDKYFKIKDKSSENYGKAIQQCKINQSYDAIQFKNNILSKSISYVKISDIEIIATLDEINTYYNHVILKPQNILKFETKTYENFKLKDIVKFSDSKSHSYHGQLVWLGEINESKAALIALNDDVVEPDIPFKLDKNFMNTNYQKDAFGIFVGFFALHHDSVTYLKLKEGNLEVIDHKYEHTFRKEPFKEKIILFNISGVQMIATLKWKGCIYNELSIGCEMNQELSEGSNGTYGTQKLFTCANKKGFLITWSKSKSLNIELCEFDSKHKELEKISTKFGFDIYDVNSICCVERLDKVDSEKHLKIGCKILFQNQSIGQCGFIGKINGRLSIGIPIDFNFIIENHSVLDFIYLYTFNHKRLCFLDFETIKIGSFYQETHCGSIAKEVKYYPEHGKCISGEVKWFGKHENGNEVCGIELDEEIREYSSKYFFENLFXXXXXXXXXXXXXXXEYVECHESSTKSIDLMGNLSLKKDEIIKCLLGTESEKHLLKYLDDLCSCYSNEFVNFLKENKQILTPQNFPQIILSFYKKNKSDAINSLLCIEETRXXXXXXXXXXXXXXXVGFFDLILPFITINSTDENNEFCLRLALKCLETINQFEESNESDNQIKTLFNKVMEKCTNPNCFINKDLGTKETFXXXXXXXXXXXXXXXXXVLKLRKFDIDKKNLKGESPLKLSIILYRPEILSTLLELNCQTDDLCSFQREEVNLPSVEGESCFRYEVLKIITRHLANETSTAINFNK 2710
            M ANDYNAAEIMIEEHQIIITND LKDAHFINDNHKNKLVKIKKTKFTGVVEHVFTLYDDKLVAVGNIYPQQNDISFQDLLLDNSEFRGFIIKDSRVKVLDSTIKFVEDSKLIDQNTIYHINFKAIEDKYFKIKDKSSENYGKAIQQCKINQSYDAIQFKNNILSKSISYVKISDIEIIATLDEINTYYNHVILKPQNILKFETKTYENFKLKDIVKFSDSKSHSYHGQLVWLGEINESKAALIALNDDVVEPDIPFKLDKNFMNTNYQKDAFGIFVGFFALHHDSVTYLKLKEGNLEVIDHKYEHTFRKEPFKEKIILFNISGVQMIATLKWKGCIYNELSIGCEMNQE+SEGSNGTYGTQKLFTCANKKGFLITWSKSKSLNIELCEFDSKHKELEKISTKFGFDIYDVNSICCVERLDKVDSEKHLKIGCKILFQNQSIGQCGFIGKINGRLSIGIPIDFNFI ENHSVLDFIYLYTFNHKRLCFLDFETIKIGSFYQETHCGSIAKEVKYYPEHGKCISGEVKWFGKHENGNEVCGIELDEEIREYSSKYFFENLFDHKDKHGIIDDDSKIEYVECHESSTKSIDLMGNLSLKKDEIIKCLLGTESEKHLLKYLDDLCSCYSNEFVNFLKENKQILTPQNFPQIILSFYKKNKSDAINSLLCIEETRSIIYSLSSLIFSDFDVGFFDLILPF+TINSTDENNEFCLRLALKCLETINQFEESNESDNQI TLFNKVMEKCTNPNCFINKDLGTKETFITEIIKNESISENIIINVLKLRKFDIDK+NLKGESPLKLSIILYRPEILSTLLELNCQTDDL SFQREEVNLPSVEGESCFRYEVLKIITRHLANETSTAINFNK
Sbjct:    1 MKANDYNAAEIMIEEHQIIITNDNLKDAHFINDNHKNKLVKIKKTKFTGVVEHVFTLYDDKLVAVGNIYPQQNDISFQDLLLDNSEFRGFIIKDSRVKVLDSTIKFVEDSKLIDQNTIYHINFKAIEDKYFKIKDKSSENYGKAIQQCKINQSYDAIQFKNNILSKSISYVKISDIEIIATLDEINTYYNHVILKPQNILKFETKTYENFKLKDIVKFSDSKSHSYHGQLVWLGEINESKAALIALNDDVVEPDIPFKLDKNFMNTNYQKDAFGIFVGFFALHHDSVTYLKLKEGNLEVIDHKYEHTFRKEPFKEKIILFNISGVQMIATLKWKGCIYNELSIGCEMNQEISEGSNGTYGTQKLFTCANKKGFLITWSKSKSLNIELCEFDSKHKELEKISTKFGFDIYDVNSICCVERLDKVDSEKHLKIGCKILFQNQSIGQCGFIGKINGRLSIGIPIDFNFINENHSVLDFIYLYTFNHKRLCFLDFETIKIGSFYQETHCGSIAKEVKYYPEHGKCISGEVKWFGKHENGNEVCGIELDEEIREYSSKYFFENLFDHKDKHGIIDDDSKIEYVECHESSTKSIDLMGNLSLKKDEIIKCLLGTESEKHLLKYLDDLCSCYSNEFVNFLKENKQILTPQNFPQIILSFYKKNKSDAINSLLCIEETRSIIYSLSSLIFSDFDVGFFDLILPFMTINSTDENNEFCLRLALKCLETINQFEESNESDNQIITLFNKVMEKCTNPNCFINKDLGTKETFITEIIKNESISENIIINVLKLRKFDIDKRNLKGESPLKLSIILYRPEILSTLLELNCQTDDLRSFQREEVNLPSVEGESCFRYEVLKIITRHLANETSTAINFNK 866          
BLAST of SMED30031905 vs. Planmine SMEST
Match: SMESG000004351.1 (SMESG000004351.1)

HSP 1 Score: 1552.34 bits (4018), Expect = 0.000e+0
Identity = 832/866 (96.07%), Postives = 835/866 (96.42%), Query Frame = 2
Query:  113 MNANDYNAAEIMIEEHQIIITNDILKDAHFINDNHKNKLVKIKKTKFTGVVEHVFTLYDDKLVAVGNIYPQQNDISFQDLLLDNSEFRGFIIKDSRVKVLDSTIKFVEDSKLIDQNTIYHINFKAIEDKYFKIKDKSSENYGKAIQQCKINQSYDAIQFKNNILSKSISYVKISDIEIIATLDEINTYYNHVILKPQNILKFETKTYENFKLKDIVKFSDSKSHSYHGQLVWLGEINESKAALIALNDDVVEPDIPFKLDKNFMNTNYQKDAFGIFVGFFALHHDSVTYLKLKEGNLEVIDHKYEHTFRKEPFKEKIILFNISGVQMIATLKWKGCIYNELSIGCEMNQELSEGSNGTYGTQKLFTCANKKGFLITWSKSKSLNIELCEFDSKHKELEKISTKFGFDIYDVNSICCVERLDKVDSEKHLKIGCKILFQNQSIGQCGFIGKINGRLSIGIPIDFNFIIENHSVLDFIYLYTFNHKRLCFLDFETIKIGSFYQETHCGSIAKEVKYYPEHGKCISGEVKWFGKHENGNEVCGIELDEEIREYSSKYFFENLFXXXXXXXXXXXXXXXEYVECHESSTKSIDLMGNLSLKKDEIIKCLLGTESEKHLLKYLDDLCSCYSNEFVNFLKENKQILTPQNFPQIILSFYKKNKSDAINSLLCIEETRXXXXXXXXXXXXXXXVGFFDLILPFITINSTDENNEFCLRLALKCLETINQFEESNESDNQIKTLFNKVMEKCTNPNCFINKDLGTKETFXXXXXXXXXXXXXXXXXVLKLRKFDIDKKNLKGESPLKLSIILYRPEILSTLLELNCQTDDLCSFQREEVNLPSVEGESCFRYEVLKIITRHLANETSTAINFNK 2710
            M ANDYNAAEIMIEEHQIIITND LKDAHFINDNHKNKLVKIKKTKFTGVVEHVFTLYDDKLVAVGNIYPQQNDISFQDLLLDNSEFRGFIIKDSRVK                          AIEDKYFKIKDKSSENYGKAIQQCKINQSYDAIQFKNNILSKSISYVKISDIEIIATLDEINTYYNHVILKPQNILKFETKTYENFKLKDIVKFSDSKSHSYHGQLVWLGEINESKAALIALNDDVVEPDIPFKLDKNFMNTNYQKDAFGIFVGFFALHHDSVTYLKLKEGNLEVIDHKYEHTFRKEPFKEKIILFNISGVQMIATLKWKGCIYNELSIGCEMNQE+SEGSNGTYGTQKLFTCANKKGFLITWSKSKSLNIELCEFDSKHKELEKISTKFGFDIYDVNSICCVERLDKVDSEKHLKIGCKILFQNQSIGQCGFIGKINGRLSIGIPIDFNFI ENHSVLDFIYLYTFNHKRLCFLDFETIKIGSFYQETHCGSIAKEVKYYPEHGKCISGEVKWFGKHENGNEVCGIELDEEIREYSSKYFFENLFDHKDKHGIIDDDSKIEYVECHESSTKSIDLMGNLSLKKDEIIKCLLGTESEKHLLKYLDDLCSCYSNEFVNFLKENKQILTPQNFPQIILSFYKKNKSDAINSLLCIEETRSIIYSLSSLIFSDFDVGFFDLILPF+TINSTDENNEFCLRLALKCLETINQFEESNESDNQI TLFNKVMEKCTNPNCFINKDLGTKETFITEIIKNESISENIIINVLKLRKFDIDK+NLKGESPLKLSIILYRPEILSTLLELNCQTDDL SFQREEVNLPSVEGESCFRYEVLKIITRHLANETSTAINFNK
Sbjct:    1 MKANDYNAAEIMIEEHQIIITNDNLKDAHFINDNHKNKLVKIKKTKFTGVVEHVFTLYDDKLVAVGNIYPQQNDISFQDLLLDNSEFRGFIIKDSRVK--------------------------AIEDKYFKIKDKSSENYGKAIQQCKINQSYDAIQFKNNILSKSISYVKISDIEIIATLDEINTYYNHVILKPQNILKFETKTYENFKLKDIVKFSDSKSHSYHGQLVWLGEINESKAALIALNDDVVEPDIPFKLDKNFMNTNYQKDAFGIFVGFFALHHDSVTYLKLKEGNLEVIDHKYEHTFRKEPFKEKIILFNISGVQMIATLKWKGCIYNELSIGCEMNQEISEGSNGTYGTQKLFTCANKKGFLITWSKSKSLNIELCEFDSKHKELEKISTKFGFDIYDVNSICCVERLDKVDSEKHLKIGCKILFQNQSIGQCGFIGKINGRLSIGIPIDFNFINENHSVLDFIYLYTFNHKRLCFLDFETIKIGSFYQETHCGSIAKEVKYYPEHGKCISGEVKWFGKHENGNEVCGIELDEEIREYSSKYFFENLFDHKDKHGIIDDDSKIEYVECHESSTKSIDLMGNLSLKKDEIIKCLLGTESEKHLLKYLDDLCSCYSNEFVNFLKENKQILTPQNFPQIILSFYKKNKSDAINSLLCIEETRSIIYSLSSLIFSDFDVGFFDLILPFMTINSTDENNEFCLRLALKCLETINQFEESNESDNQIITLFNKVMEKCTNPNCFINKDLGTKETFITEIIKNESISENIIINVLKLRKFDIDKRNLKGESPLKLSIILYRPEILSTLLELNCQTDDLRSFQREEVNLPSVEGESCFRYEVLKIITRHLANETSTAINFNK 840          
BLAST of SMED30031905 vs. Planmine SMEST
Match: SMESG000044783.1 (SMESG000044783.1)

HSP 1 Score: 80.1073 bits (196), Expect = 5.005e-15
Identity = 83/301 (27.57%), Postives = 136/301 (45.18%), Query Frame = 2
Query:  956 LHHDSVTYLK-LKEGNLEVIDHKYEHTFRKEPFKEKIILFNISGVQMIATLKWKGCIYNELSIGCEMNQELSEGSNGTYGTQKLFTCANKKGFLITWSKSKSLNIELCEFD--SKHKELEKISTKFGFDIYDVNS--ICCVERLDKVDSEKHLKIGCKILFQN----QSIGQCGFIGKINGRLSIGIPIDFNFIIENHSVLDFIYLYT--------------FNHK---RLCFLDFETIKIGSFYQETHCG-----SIAKEVKY---YPEHGKC-ISGEVKWFGKHENGNEVCGIELDEEI 1753
            L + S + LK +KE NL V+D              K+  +   G   I  +KW+G +  EL +GCE++  +  G++GT+G + +F C  ++G LI    ++ L I+ CE    SK   L      +  D Y +N   ICC E  + +     L+I  +I   N    Q  G C +IG ING+ ++GI +D N +   + +   + ++T              F+ K   +  F+  + + I   Y    C      SI K +K     P   K    GEVKW G+  N  +  G++++EE+
Sbjct:  312 LANKSKSELKYVKEINLNVVDPFNVREIENGRRINKLTRYKSKGAIKILNIKWRGIVNGELCVGCELSGPVEWGTDGTFGNKIVFKCPARRGVLIP---TEEL-IDSCETTAHSKITTLPDSGWWWKGDTYFINPDCICCTESTELLQKHPDLRIKSRIKVPNKENKQIGGSCEWIGLINGQEALGITMD-NDVKGTNGIFSCLRMFTCEFGKGVLMLAENIFHDKESEKPQFIADKIVDINKIYSIFPCEGENGISIGKPIKINLNSPNGRKRDFIGEVKWTGRI-NDEKCVGLDMEEEM 606          
BLAST of SMED30031905 vs. Planmine SMEST
Match: SMESG000044783.1 (SMESG000044783.1)

HSP 1 Score: 80.1073 bits (196), Expect = 5.034e-15
Identity = 83/301 (27.57%), Postives = 136/301 (45.18%), Query Frame = 2
Query:  956 LHHDSVTYLK-LKEGNLEVIDHKYEHTFRKEPFKEKIILFNISGVQMIATLKWKGCIYNELSIGCEMNQELSEGSNGTYGTQKLFTCANKKGFLITWSKSKSLNIELCEFD--SKHKELEKISTKFGFDIYDVNS--ICCVERLDKVDSEKHLKIGCKILFQN----QSIGQCGFIGKINGRLSIGIPIDFNFIIENHSVLDFIYLYT--------------FNHK---RLCFLDFETIKIGSFYQETHCG-----SIAKEVKY---YPEHGKC-ISGEVKWFGKHENGNEVCGIELDEEI 1753
            L + S + LK +KE NL V+D              K+  +   G   I  +KW+G +  EL +GCE++  +  G++GT+G + +F C  ++G LI    ++ L I+ CE    SK   L      +  D Y +N   ICC E  + +     L+I  +I   N    Q  G C +IG ING+ ++GI +D N +   + +   + ++T              F+ K   +  F+  + + I   Y    C      SI K +K     P   K    GEVKW G+  N  +  G++++EE+
Sbjct:  312 LANKSKSELKYVKEINLNVVDPFNVREIENGRRINKLTRYKSKGAIKILNIKWRGIVNGELCVGCELSGPVEWGTDGTFGNKIVFKCPARRGVLIP---TEEL-IDSCETTAHSKITTLPDSGWWWKGDTYFINPDCICCTESTELLQKHPDLRIKSRIKVPNKENKQIGGSCEWIGLINGQEALGITMD-NDVKGTNGIFSCLRMFTCEFGKGVLMLAENIFHDKESEKPQFIADKIVDINKIYSIFPCEGENGISIGKPIKINLNSPNGRKRDFIGEVKWTGRI-NDEKCVGLDMEEEM 606          
BLAST of SMED30031905 vs. Planmine SMEST
Match: SMESG000044783.1 (SMESG000044783.1)

HSP 1 Score: 79.7221 bits (195), Expect = 5.665e-15
Identity = 82/301 (27.24%), Postives = 136/301 (45.18%), Query Frame = 2
Query:  956 LHHDSVTYLK-LKEGNLEVIDHKYEHTFRKEPFKEKIILFNISGVQMIATLKWKGCIYNELSIGCEMNQELSEGSNGTYGTQKLFTCANKKGFLITWSKSKSLNIELCEFDSKHK--ELEKISTKFGFDIYDVNS--ICCVERLDKVDSEKHLKIGCKILFQN----QSIGQCGFIGKINGRLSIGIPIDFNFIIENHSVLDFIYLYT--------------FNHK---RLCFLDFETIKIGSFYQETHCG-----SIAKEVKY---YPEHGKC-ISGEVKWFGKHENGNEVCGIELDEEI 1753
            L + S + LK +KE NL V+D              K+  +   G   I  +KW+G +  EL +GCE++  +  G++GT+G + +F C  ++G LI    ++ L I+ CE  +  K   L      +  D Y +N   ICC E  + +     L+I  +I   N    Q  G C +IG ING+ ++GI +D N +   + +   + ++T              F+ K   +  F+  + + I   Y    C      SI K +K     P   K    GEVKW G+  N  +  G++++EE+
Sbjct:  312 LANKSKSELKYVKEINLNVVDPFNVREIENGRRINKLTRYKSKGAIKILNIKWRGIVNGELCVGCELSGPVEWGTDGTFGNKIVFKCPARRGVLIP---TEEL-IDSCETTAHSKITTLPDSGWWWKGDTYFINPDCICCTESTELLQKHPDLRIKSRIKVPNKENKQIGGSCEWIGLINGQEALGITMD-NDVKGTNGIFSCLRMFTCEFGKGVLMLAENIFHDKESEKPQFIADKIVDINKIYSIFPCEGENGISIGKPIKINLNSPNGRKRDFIGEVKWTGRI-NDEKCVGLDMEEEM 606          
The following BLAST results are available for this feature:
BLAST of SMED30031905 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031905 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031905 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031905 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031905 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031905 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031905 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031905 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031905 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031905 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031905 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031905 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031905 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031905 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000004351.10.000e+099.08SMESG000004351.1[more]
SMESG000004351.10.000e+096.07SMESG000004351.1[more]
SMESG000044783.15.005e-1527.57SMESG000044783.1[more]
SMESG000044783.15.034e-1527.57SMESG000044783.1[more]
SMESG000044783.15.665e-1527.24SMESG000044783.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031905 ID=SMED30031905|Name=SMED30031905|organism=Schmidtea mediterranea sexual|type=transcript|length=2783bp
TTTTAGGTAAAATTTAATTTCTTATTTCTTGTATAGTTAAAAAAAAATTT
TTACACTATTTTAAAGATAGTAGTTCTAATCTTTTATAGTATATGATTTT
TAGAATTTTAAAATGAATGCAAATGACTATAATGCTGCTGAAATTATGAT
TGAAGAACATCAAATCATTATTACAAATGACATTTTAAAAGATGCTCATT
TTATAAATGACAATCATAAAAATAAACTTGTAAAGATTAAGAAAACTAAA
TTTACCGGTGTGGTTGAGCACGTATTTACTTTGTATGATGATAAGCTCGT
TGCTGTTGGTAACATTTATCCCCAACAAAATGATATTAGTTTTCAAGATC
TACTTCTTGATAATTCTGAATTTCGAGGATTTATTATAAAGGATTCCCGT
GTAAAGGTTTTAGATAGCACTATAAAATTTGTAGAAGATTCAAAATTAAT
CGATCAAAATACAATTTATCATATAAATTTTAAGGCGATTGAAGACAAGT
ATTTCAAAATAAAAGATAAATCTAGTGAAAACTATGGAAAGGCTATACAG
CAATGTAAAATAAATCAATCTTATGATGCAATTCAATTTAAAAATAACAT
TTTATCAAAATCTATTTCCTATGTCAAAATCTCGGATATTGAAATCATCG
CAACTTTGGATGAAATAAATACGTATTATAATCACGTTATATTAAAGCCT
CAAAATATATTGAAATTTGAAACAAAGACATATGAAAATTTTAAATTAAA
GGATATAGTGAAATTTTCGGACTCTAAATCGCATAGTTATCATGGACAAT
TAGTTTGGCTTGGTGAAATAAATGAAAGTAAAGCAGCATTAATAGCTTTG
AATGACGATGTTGTTGAACCAGATATACCATTTAAACTCGATAAAAATTT
TATGAATACTAACTATCAAAAAGATGCATTTGGAATTTTCGTAGGATTTT
TCGCTTTGCATCACGATTCTGTTACATACTTAAAACTGAAAGAAGGAAAT
TTAGAAGTGATTGATCATAAATATGAACATACATTTCGAAAAGAACCTTT
CAAAGAAAAAATAATTTTATTTAATATATCTGGTGTTCAAATGATTGCTA
CATTGAAATGGAAGGGATGTATTTATAACGAATTGAGTATCGGTTGTGAG
ATGAATCAAGAACTCAGTGAAGGTAGTAATGGTACGTATGGTACTCAAAA
GTTGTTTACATGTGCTAACAAAAAGGGATTTTTGATAACATGGAGTAAAT
CAAAATCTCTAAACATAGAATTATGTGAATTTGATTCAAAACATAAGGAA
CTTGAAAAAATTTCCACTAAATTTGGATTTGACATTTATGATGTGAATTC
TATATGTTGTGTTGAAAGATTAGATAAAGTTGATAGTGAAAAACATCTTA
AAATTGGTTGTAAAATATTATTTCAAAACCAAAGTATTGGTCAATGTGGG
TTTATTGGAAAAATTAATGGAAGGCTGAGTATTGGAATTCCAATAGATTT
TAATTTCATCATTGAAAACCACTCTGTTCTTGATTTTATTTATTTATATA
CCTTTAATCATAAAAGGTTATGCTTTTTAGACTTTGAAACTATTAAAATT
GGTTCATTTTATCAAGAAACACATTGTGGTTCAATTGCCAAAGAAGTTAA
ATATTATCCAGAACATGGAAAATGTATAAGTGGTGAAGTTAAATGGTTTG
GTAAGCATGAAAATGGTAATGAGGTTTGTGGAATCGAATTAGACGAGGAA
ATTAGAGAATATAGTAGTAAATATTTTTTCGAGAATTTATTTGATCATAA
AGATAAACATGGCATAATCGATGATGACAGCAAGATTGAATATGTGGAAT
GCCATGAATCTTCGACTAAATCTATTGATTTGATGGGAAATCTCTCTTTA
AAGAAAGATGAAATCATAAAATGCTTGTTGGGAACAGAATCGGAGAAGCA
CTTACTGAAATATTTAGATGATTTATGTTCATGTTATTCAAATGAATTCG
TTAATTTTTTGAAAGAGAATAAACAAATATTGACACCTCAAAATTTTCCA
CAGATTATATTGTCATTTTACAAAAAAAATAAATCTGATGCTATAAATTC
ACTTCTATGTATTGAAGAAACCAGATCAATAATTTATTCTCTTTCTTCAC
TTATTTTCAGTGATTTTGACGTGGGTTTCTTTGATTTAATTTTACCTTTC
ATAACGATAAATTCTACTGATGAAAATAATGAATTTTGTTTACGTTTGGC
ATTGAAATGCCTAGAAACTATCAACCAATTCGAAGAATCAAATGAGTCTG
ATAATCAAATTAAAACTCTATTCAATAAAGTAATGGAAAAATGCACAAAT
CCGAATTGTTTTATCAACAAAGACTTGGGAACAAAAGAAACATTTATAAC
TGAAATCATAAAAAATGAGTCAATATCTGAAAACATTATAATAAACGTGT
TGAAATTACGAAAATTTGATATCGATAAAAAAAATTTGAAAGGTGAATCT
CCTTTGAAATTATCTATTATATTATATCGACCTGAGATTCTTTCTACATT
ACTCGAACTAAATTGTCAAACTGATGACCTTTGTTCTTTTCAACGGGAAG
AGGTGAATTTGCCGTCAGTGGAGGGAGAATCATGCTTTCGGTATGAGGTT
TTAAAAATAATTACTCGACATCTAGCAAATGAAACTTCGACGGCAATAAA
TTTTAATAAGTAGTTGTATTTACATTTTGATTTTGATTGATAAAAATTAC
TTCATTGTAAAGTATTTTTAAAATACATTTAGC
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protein sequence of SMED30031905-orf-1

>SMED30031905-orf-1 ID=SMED30031905-orf-1|Name=SMED30031905-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=867bp
MNANDYNAAEIMIEEHQIIITNDILKDAHFINDNHKNKLVKIKKTKFTGV
VEHVFTLYDDKLVAVGNIYPQQNDISFQDLLLDNSEFRGFIIKDSRVKVL
DSTIKFVEDSKLIDQNTIYHINFKAIEDKYFKIKDKSSENYGKAIQQCKI
NQSYDAIQFKNNILSKSISYVKISDIEIIATLDEINTYYNHVILKPQNIL
KFETKTYENFKLKDIVKFSDSKSHSYHGQLVWLGEINESKAALIALNDDV
VEPDIPFKLDKNFMNTNYQKDAFGIFVGFFALHHDSVTYLKLKEGNLEVI
DHKYEHTFRKEPFKEKIILFNISGVQMIATLKWKGCIYNELSIGCEMNQE
LSEGSNGTYGTQKLFTCANKKGFLITWSKSKSLNIELCEFDSKHKELEKI
STKFGFDIYDVNSICCVERLDKVDSEKHLKIGCKILFQNQSIGQCGFIGK
INGRLSIGIPIDFNFIIENHSVLDFIYLYTFNHKRLCFLDFETIKIGSFY
QETHCGSIAKEVKYYPEHGKCISGEVKWFGKHENGNEVCGIELDEEIREY
SSKYFFENLFDHKDKHGIIDDDSKIEYVECHESSTKSIDLMGNLSLKKDE
IIKCLLGTESEKHLLKYLDDLCSCYSNEFVNFLKENKQILTPQNFPQIIL
SFYKKNKSDAINSLLCIEETRSIIYSLSSLIFSDFDVGFFDLILPFITIN
STDENNEFCLRLALKCLETINQFEESNESDNQIKTLFNKVMEKCTNPNCF
INKDLGTKETFITEIIKNESISENIIINVLKLRKFDIDKKNLKGESPLKL
SIILYRPEILSTLLELNCQTDDLCSFQREEVNLPSVEGESCFRYEVLKII
TRHLANETSTAINFNK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR036859CAP-Gly_dom_sf
IPR000938CAP-Gly_domain
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036859CAP Gly-rich domain superfamilyGENE3DG3DSA:2.30.30.190coord: 317..393
e-value: 2.9E-5
score: 25.5
IPR036859CAP Gly-rich domain superfamilySUPERFAMILYSSF74924Cap-Gly domaincoord: 322..379