SMED30031902

Overview
NameSMED30031902
Smed IDSMED30031902
Length (bp)307
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30031902 (SMED30031902) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30031902 (SMED30031902) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30031902 (SMED30031902) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30031902 (SMED30031902) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30031902

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 33

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30031902h1SMcG0006857 Contig33875newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031902h1SMcG0006857 Contig33875uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031902h1SMcG0006857 Contig31778newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031902h1SMcG0006857 Contig31778uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031902h1SMcG0006857 Contig33876uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031902h1SMcG0006857 Contig33876newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031902h1SMcG0006857 Contig37769newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031902h1SMcG0006857 Contig37769uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30031902h1SMcG0006857 dd_Smed_v4_1410_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30031902h1SMcG0006857 dd_Smed_v4_1410_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30031902h1SMcG0006857 dd_Smed_v4_1410_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30031902h1SMcG0006857 dd_Smed_v4_1410_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30031902h1SMcG0006857 dd_Smed_v4_1410_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30031902h1SMcG0006857 dd_Smed_v4_2869_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30031902h1SMcG0006857 dd_Smed_v4_2869_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30031902h1SMcG0006857 dd_Smed_v4_2869_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30031902h1SMcG0006857 dd_Smed_v4_2869_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031902h1SMcG0006857 Contig45224newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031902h1SMcG0006857 Contig45224uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30031902h1SMcG0006857 dd_Smed_v4_6915_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30031902h1SMcG0006857 dd_Smed_v4_6915_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30031902h1SMcG0006857 dd_Smed_v4_6915_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30031902h1SMcG0006857 dd_Smed_v4_6915_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30031902h1SMcG0006857 dd_Smed_v4_6915_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30031902h1SMcG0006857 dd_Smed_v4_6915_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031902h1SMcG0018388 Contig45224newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031902h1SMcG0018388 Contig45224uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
pharynx musculatureSMED30031902h1SMcG0006857 dd_Smed_v6_2869_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
musculature systemSMED30031902h1SMcG0006857 dd_Smed_v6_2869_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynx musculatureSMED30031902h1SMcG0006857 dd_Smed_v6_6915_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
musculature systemSMED30031902h1SMcG0006857 dd_Smed_v6_6915_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle progenitor cellSMED30031902h1SMcG0006857 dd_Smed_v6_6915_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30031902h1SMcG0006857 dd_Smed_v4_6915_0_1dd_Smed_v4PMID:30962434
Cote et al., 2019		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30031902 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001097:1240..2075 +1333
Homology
[Close]
BLAST of SMED30031902 vs. Planmine SMEST
Match: SMESG000055070.1 (SMESG000055070.1)

HSP 1 Score: 186.808 bits (473), Expect = 3.202e-56
Identity = 90/102 (88.24%), Postives = 95/102 (93.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKAE P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 4870 ADKQEYPIKDLADKVGINGGDISFLSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 4971

HSP 2 Score: 186.808 bits (473), Expect = 3.813e-56
Identity = 90/102 (88.24%), Postives = 95/102 (93.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKAE P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 3776 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 3877

HSP 3 Score: 186.808 bits (473), Expect = 3.813e-56
Identity = 90/102 (88.24%), Postives = 95/102 (93.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKAE P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 4414 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 4515

HSP 4 Score: 186.808 bits (473), Expect = 3.813e-56
Identity = 90/102 (88.24%), Postives = 95/102 (93.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKAE P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 4642 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 4743

HSP 5 Score: 184.496 bits (467), Expect = 2.122e-55
Identity = 89/102 (87.25%), Postives = 95/102 (93.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKAE P+GVRFVV GLD   +FNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 3548 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAESPAGVRFVVTGLDRDSSFNPKQGSMDRKLQASIIDAKTGKDL 3649

HSP 6 Score: 152.525 bits (384), Expect = 3.183e-44
Identity = 78/108 (72.22%), Postives = 85/108 (78.70%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKA------EFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA      E P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 3890 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKAVLLSIPESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 3997

HSP 7 Score: 137.502 bits (345), Expect = 5.961e-39
Identity = 68/94 (72.34%), Postives = 78/94 (82.98%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 4300 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 4393

HSP 8 Score: 111.694 bits (278), Expect = 6.239e-30
Identity = 58/98 (59.18%), Postives = 70/98 (71.43%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAK 292
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I D K
Sbjct: 4010 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYIEDDK 4107

HSP 9 Score: 111.694 bits (278), Expect = 7.218e-30
Identity = 57/94 (60.64%), Postives = 68/94 (72.34%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KAE  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 4984 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKAETQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 5077

HSP 10 Score: 110.153 bits (274), Expect = 2.602e-29
Identity = 56/94 (59.57%), Postives = 68/94 (72.34%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 4528 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 4621

HSP 11 Score: 110.153 bits (274), Expect = 2.602e-29
Identity = 56/94 (59.57%), Postives = 68/94 (72.34%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 4756 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 4849

HSP 12 Score: 109.768 bits (273), Expect = 3.727e-29
Identity = 55/94 (58.51%), Postives = 68/94 (72.34%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K +  GGEI F+ MKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 3662 SNKEEYPIDKLAEKVDQKGGEIKFMLMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 3755

HSP 13 Score: 90.5077 bits (223), Expect = 1.840e-22
Identity = 43/56 (76.79%), Postives = 46/56 (82.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDV 169
            +DKQEYPI DLADK  INGGEISF+SMKNPQQVLTAQCFAT GA N+TS    V V
Sbjct: 5098 ADKQEYPIMDLADKVVINGGEISFMSMKNPQQVLTAQCFATKGAHNYTSTRISVTV 5153

HSP 14 Score: 84.3445 bits (207), Expect = 2.495e-20
Identity = 43/71 (60.56%), Postives = 51/71 (71.83%), Query Frame = 2 
Query:   71 FISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 4194 FNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 4264

HSP 15 Score: 81.6481 bits (200), Expect = 2.225e-19
Identity = 54/113 (47.79%), Postives = 66/113 (58.41%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEI--SFISMKNPQQVLTAQCFATNGAQNFTSHSFLVD-----VSKAEF----PSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +D   Y IKD + + E+   EI  +F   +    +LT +        N T +  + D     +S   F    PSGVRFVVAGLDG  +FNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 3346 TDVTWYQIKD-SQEVELLKSEIKDNFNVYQKQPNILTVKILNMKKNSNMTLNCVVKDANGVMISSPVFLTQSPSGVRFVVAGLDGDSSFNPKQGSMDRKLQASIIDAKTGKDL 3457

HSP 16 Score: 78.1814 bits (191), Expect = 3.523e-18
Identity = 36/54 (66.67%), Postives = 42/54 (77.78%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLV 163
            S+K+EYPI  LA+K +  GGEI F+ MKNPQQ+LTAQCFATNG  NFTS S  V
Sbjct: 3470 SNKEEYPIDKLAEKVDQKGGEIKFMLMKNPQQILTAQCFATNGTHNFTSPSITV 3523

HSP 17 Score: 77.411 bits (189), Expect = 7.028e-18
Identity = 40/59 (67.80%), Postives = 42/59 (71.19%), Query Frame = 3 
Query:    3 RTNKNIQSKIWQTNLR*MEEKSHSFQ*RIHNKFSRLSALRQMGLRTLHLIPSLWTSVKQ 179
            RTNKNIQS+IWQT                 NKFSRLSALRQMGLRT+HLIPSLWTSVKQ
Sbjct: 4124 RTNKNIQSRIWQTKSDKWRRHLIYVNEESANKFSRLSALRQMGLRTIHLIPSLWTSVKQ 4182

HSP 18 Score: 63.1586 bits (152), Expect = 8.774e-13
Identity = 32/82 (39.02%), Postives = 46/82 (56.10%), Query Frame = 2 
Query:   17 YPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDR 262
            YPIKDLA+      G++SF+++KNP+QVL AQC   NG+ N  S  F + V + E      F+    +G LN   +   +DR
Sbjct: 3152 YPIKDLAENLIEKDGKLSFMTVKNPRQVLYAQCRVNNGSVNDVSKPFFIHVDEDELKRPKLFLKPTFEGELNAVKRLDCIDR 3233
[Close]
BLAST of SMED30031902 vs. Planmine SMEST
Match: SMESG000055070.1 (SMESG000055070.1)

HSP 1 Score: 186.808 bits (473), Expect = 3.265e-56
Identity = 90/102 (88.24%), Postives = 95/102 (93.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKAE P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 4935 ADKQEYPIKDLADKVGINGGDISFLSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 5036

HSP 2 Score: 186.808 bits (473), Expect = 3.887e-56
Identity = 90/102 (88.24%), Postives = 95/102 (93.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKAE P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 4479 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 4580

HSP 3 Score: 186.808 bits (473), Expect = 3.887e-56
Identity = 90/102 (88.24%), Postives = 95/102 (93.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKAE P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 4707 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 4808

HSP 4 Score: 184.496 bits (467), Expect = 2.163e-55
Identity = 89/102 (87.25%), Postives = 95/102 (93.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKAE P+GVRFVV GLD   +FNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 3548 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAESPAGVRFVVTGLDRDSSFNPKQGSMDRKLQASIIDAKTGKDL 3649

HSP 5 Score: 180.644 bits (457), Expect = 4.865e-54
Identity = 90/108 (83.33%), Postives = 95/108 (87.96%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEF------PSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKA F      P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 3776 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAVFLSIPESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 3883

HSP 6 Score: 137.502 bits (345), Expect = 6.138e-39
Identity = 68/94 (72.34%), Postives = 78/94 (82.98%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 4365 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 4458

HSP 7 Score: 111.694 bits (278), Expect = 6.301e-30
Identity = 58/98 (59.18%), Postives = 70/98 (71.43%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAK 292
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I D K
Sbjct: 4075 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYIEDDK 4172

HSP 8 Score: 111.694 bits (278), Expect = 7.360e-30
Identity = 57/94 (60.64%), Postives = 68/94 (72.34%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KAE  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 5049 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKAETQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 5142

HSP 9 Score: 110.153 bits (274), Expect = 2.653e-29
Identity = 56/94 (59.57%), Postives = 68/94 (72.34%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 4593 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 4686

HSP 10 Score: 110.153 bits (274), Expect = 2.653e-29
Identity = 56/94 (59.57%), Postives = 68/94 (72.34%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 4821 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 4914

HSP 11 Score: 109.768 bits (273), Expect = 3.838e-29
Identity = 55/94 (58.51%), Postives = 68/94 (72.34%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K +  GGEI F+ MKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 3662 SNKEEYPIDKLAEKVDQKGGEIKFMLMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 3755

HSP 12 Score: 108.612 bits (270), Expect = 1.004e-28
Identity = 64/114 (56.14%), Postives = 72/114 (63.16%), Query Frame = 2 
Query:    2 SDKQEYPIKDLA----DKFEINGGEISFISMKNPQQVLTAQ--CFATNGAQNFTSHSFLVDVSKA------EFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKD      DK+         +   N +   ++         AQN+TSHSFLVDVSKA      E P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 3955 ADKQEYPIKDFGRQSRDKWR------RHLIYVNEESATSSHGSVLCDKWAQNYTSHSFLVDVSKAVLLSIPESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 4062

HSP 13 Score: 90.5077 bits (223), Expect = 1.858e-22
Identity = 43/56 (76.79%), Postives = 46/56 (82.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDV 169
            +DKQEYPI DLADK  INGGEISF+SMKNPQQVLTAQCFAT GA N+TS    V V
Sbjct: 5163 ADKQEYPIMDLADKVVINGGEISFMSMKNPQQVLTAQCFATKGAHNYTSTRISVTV 5218

HSP 14 Score: 87.0409 bits (214), Expect = 3.639e-21
Identity = 43/66 (65.15%), Postives = 50/66 (75.76%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKA----EFP 187
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA    E+P
Sbjct: 3896 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKAADKQEYP 3961

HSP 15 Score: 84.3445 bits (207), Expect = 2.544e-20
Identity = 43/71 (60.56%), Postives = 51/71 (71.83%), Query Frame = 2 
Query:   71 FISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 4259 FNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 4329

HSP 16 Score: 81.6481 bits (200), Expect = 2.313e-19
Identity = 54/113 (47.79%), Postives = 66/113 (58.41%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEI--SFISMKNPQQVLTAQCFATNGAQNFTSHSFLVD-----VSKAEF----PSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +D   Y IKD + + E+   EI  +F   +    +LT +        N T +  + D     +S   F    PSGVRFVVAGLDG  +FNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 3346 TDVTWYQIKD-SQEVELLKSEIKDNFNVYQKQPNILTVKILNMKKNSNMTLNCVVKDANGVMISSPVFLTQSPSGVRFVVAGLDGDSSFNPKQGSMDRKLQASIIDAKTGKDL 3457

HSP 17 Score: 78.1814 bits (191), Expect = 3.592e-18
Identity = 36/54 (66.67%), Postives = 42/54 (77.78%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLV 163
            S+K+EYPI  LA+K +  GGEI F+ MKNPQQ+LTAQCFATNG  NFTS S  V
Sbjct: 3470 SNKEEYPIDKLAEKVDQKGGEIKFMLMKNPQQILTAQCFATNGTHNFTSPSITV 3523

HSP 18 Score: 77.411 bits (189), Expect = 7.451e-18
Identity = 40/59 (67.80%), Postives = 42/59 (71.19%), Query Frame = 3 
Query:    3 RTNKNIQSKIWQTNLR*MEEKSHSFQ*RIHNKFSRLSALRQMGLRTLHLIPSLWTSVKQ 179
            RTNKNIQS+IWQT                 NKFSRLSALRQMGLRT+HLIPSLWTSVKQ
Sbjct: 4189 RTNKNIQSRIWQTKSDKWRRHLIYVNEESANKFSRLSALRQMGLRTIHLIPSLWTSVKQ 4247

HSP 19 Score: 62.7734 bits (151), Expect = 9.035e-13
Identity = 32/82 (39.02%), Postives = 46/82 (56.10%), Query Frame = 2 
Query:   17 YPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDR 262
            YPIKDLA+      G++SF+++KNP+QVL AQC   NG+ N  S  F + V + E      F+    +G LN   +   +DR
Sbjct: 3152 YPIKDLAENLIEKDGKLSFMTVKNPRQVLYAQCRVNNGSVNDVSKPFFIHVDEDELKRPKLFLKPTFEGELNAVKRLDCIDR 3233
[Close]
BLAST of SMED30031902 vs. Planmine SMEST
Match: SMESG000055070.1 (SMESG000055070.1)

HSP 1 Score: 186.808 bits (473), Expect = 3.329e-56
Identity = 90/102 (88.24%), Postives = 95/102 (93.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKAE P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 4880 ADKQEYPIKDLADKVGINGGDISFLSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 4981

HSP 2 Score: 186.808 bits (473), Expect = 3.963e-56
Identity = 90/102 (88.24%), Postives = 95/102 (93.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKAE P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 3776 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 3877

HSP 3 Score: 186.808 bits (473), Expect = 3.963e-56
Identity = 90/102 (88.24%), Postives = 95/102 (93.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKAE P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 4198 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 4299

HSP 4 Score: 184.496 bits (467), Expect = 2.205e-55
Identity = 89/102 (87.25%), Postives = 95/102 (93.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKAE P+GVRFVV GLD   +FNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 3548 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAESPAGVRFVVTGLDRDSSFNPKQGSMDRKLQASIIDAKTGKDL 3649

HSP 5 Score: 180.644 bits (457), Expect = 4.960e-54
Identity = 90/108 (83.33%), Postives = 95/108 (87.96%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEF------PSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKA F      P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 4412 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAVFLSIPESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 4519

HSP 6 Score: 180.644 bits (457), Expect = 4.960e-54
Identity = 90/108 (83.33%), Postives = 95/108 (87.96%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEF------PSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKA F      P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 4646 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAVFLSIPESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 4753

HSP 7 Score: 111.694 bits (278), Expect = 7.504e-30
Identity = 57/94 (60.64%), Postives = 68/94 (72.34%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KAE  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 4994 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKAETQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 5087

HSP 8 Score: 110.153 bits (274), Expect = 2.705e-29
Identity = 56/94 (59.57%), Postives = 68/94 (72.34%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 4069 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 4162

HSP 9 Score: 110.153 bits (274), Expect = 2.705e-29
Identity = 56/94 (59.57%), Postives = 68/94 (72.34%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 4532 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 4625

HSP 10 Score: 110.153 bits (274), Expect = 2.705e-29
Identity = 56/94 (59.57%), Postives = 68/94 (72.34%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 4766 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 4859

HSP 11 Score: 109.768 bits (273), Expect = 3.875e-29
Identity = 55/94 (58.51%), Postives = 68/94 (72.34%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K +  GGEI F+ MKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 3662 SNKEEYPIDKLAEKVDQKGGEIKFMLMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 3755

HSP 12 Score: 108.227 bits (269), Expect = 1.064e-28
Identity = 64/114 (56.14%), Postives = 72/114 (63.16%), Query Frame = 2 
Query:    2 SDKQEYPIKDLA----DKFEINGGEISFISMKNPQQVLTAQ--CFATNGAQNFTSHSFLVDVSKA------EFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKD      DK+         +   N +   ++         AQN+TSHSFLVDVSKA      E P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 3949 ADKQEYPIKDFGRQSRDKWR------RHLIYVNEESATSSHGSVLCDKWAQNYTSHSFLVDVSKAVLLSIPESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 4056

HSP 13 Score: 90.5077 bits (223), Expect = 1.876e-22
Identity = 43/56 (76.79%), Postives = 46/56 (82.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDV 169
            +DKQEYPI DLADK  INGGEISF+SMKNPQQVLTAQCFAT GA N+TS    V V
Sbjct: 5108 ADKQEYPIMDLADKVVINGGEISFMSMKNPQQVLTAQCFATKGAHNYTSTRISVTV 5163

HSP 14 Score: 87.0409 bits (214), Expect = 2.938e-21
Identity = 48/94 (51.06%), Postives = 59/94 (62.77%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K              NPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 4312 SNKEEYPIDKLAEK--------------NPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 4391

HSP 15 Score: 86.6557 bits (213), Expect = 3.783e-21
Identity = 43/66 (65.15%), Postives = 50/66 (75.76%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKA----EFP 187
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA    E+P
Sbjct: 3890 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKAADKQEYP 3955

HSP 16 Score: 81.6481 bits (200), Expect = 2.313e-19
Identity = 54/113 (47.79%), Postives = 66/113 (58.41%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEI--SFISMKNPQQVLTAQCFATNGAQNFTSHSFLVD-----VSKAEF----PSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +D   Y IKD + + E+   EI  +F   +    +LT +        N T +  + D     +S   F    PSGVRFVVAGLDG  +FNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 3346 TDVTWYQIKD-SQEVELLKSEIKDNFNVYQKQPNILTVKILNMKKNSNMTLNCVVKDANGVMISSPVFLTQSPSGVRFVVAGLDGDSSFNPKQGSMDRKLQASIIDAKTGKDL 3457

HSP 17 Score: 78.1814 bits (191), Expect = 3.627e-18
Identity = 36/54 (66.67%), Postives = 42/54 (77.78%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLV 163
            S+K+EYPI  LA+K +  GGEI F+ MKNPQQ+LTAQCFATNG  NFTS S  V
Sbjct: 3470 SNKEEYPIDKLAEKVDQKGGEIKFMLMKNPQQILTAQCFATNGTHNFTSPSITV 3523

HSP 18 Score: 62.7734 bits (151), Expect = 9.302e-13
Identity = 32/82 (39.02%), Postives = 46/82 (56.10%), Query Frame = 2 
Query:   17 YPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDR 262
            YPIKDLA+      G++SF+++KNP+QVL AQC   NG+ N  S  F + V + E      F+    +G LN   +   +DR
Sbjct: 3152 YPIKDLAENLIEKDGKLSFMTVKNPRQVLYAQCRVNNGSVNDVSKPFFIHVDEDELKRPKLFLKPTFEGELNAVKRLDCIDR 3233
[Close]
BLAST of SMED30031902 vs. Planmine SMEST
Match: SMESG000055070.1 (SMESG000055070.1)

HSP 1 Score: 186.808 bits (473), Expect = 3.329e-56
Identity = 90/102 (88.24%), Postives = 95/102 (93.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKAE P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 4982 ADKQEYPIKDLADKVGINGGDISFLSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 5083

HSP 2 Score: 186.808 bits (473), Expect = 3.964e-56
Identity = 90/102 (88.24%), Postives = 95/102 (93.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKAE P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 3776 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 3877

HSP 3 Score: 186.808 bits (473), Expect = 3.964e-56
Identity = 90/102 (88.24%), Postives = 95/102 (93.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKAE P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 4754 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 4855

HSP 4 Score: 184.496 bits (467), Expect = 2.206e-55
Identity = 89/102 (87.25%), Postives = 95/102 (93.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKAE P+GVRFVV GLD   +FNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 3548 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAESPAGVRFVVTGLDRDSSFNPKQGSMDRKLQASIIDAKTGKDL 3649

HSP 5 Score: 180.644 bits (457), Expect = 4.960e-54
Identity = 90/108 (83.33%), Postives = 95/108 (87.96%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEF------PSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKA F      P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 4300 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAVFLSIPESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 4407

HSP 6 Score: 180.644 bits (457), Expect = 4.960e-54
Identity = 90/108 (83.33%), Postives = 95/108 (87.96%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEF------PSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKA F      P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 4520 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAVFLSIPESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 4627

HSP 7 Score: 152.525 bits (384), Expect = 3.309e-44
Identity = 78/108 (72.22%), Postives = 85/108 (78.70%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKA------EFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA      E P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 3890 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKAVLLSIPESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 3997

HSP 8 Score: 111.694 bits (278), Expect = 6.301e-30
Identity = 58/98 (59.18%), Postives = 70/98 (71.43%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAK 292
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I D K
Sbjct: 4010 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYIEDDK 4107

HSP 9 Score: 111.694 bits (278), Expect = 7.361e-30
Identity = 57/94 (60.64%), Postives = 68/94 (72.34%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KAE  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 5096 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKAETQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 5189

HSP 10 Score: 110.153 bits (274), Expect = 2.653e-29
Identity = 56/94 (59.57%), Postives = 68/94 (72.34%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 4640 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 4733

HSP 11 Score: 110.153 bits (274), Expect = 2.653e-29
Identity = 56/94 (59.57%), Postives = 68/94 (72.34%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 4868 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 4961

HSP 12 Score: 109.768 bits (273), Expect = 3.801e-29
Identity = 55/94 (58.51%), Postives = 68/94 (72.34%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K +  GGEI F+ MKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 3662 SNKEEYPIDKLAEKVDQKGGEIKFMLMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 3755

HSP 13 Score: 90.5077 bits (223), Expect = 1.876e-22
Identity = 43/56 (76.79%), Postives = 46/56 (82.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDV 169
            +DKQEYPI DLADK  INGGEISF+SMKNPQQVLTAQCFAT GA N+TS    V V
Sbjct: 5210 ADKQEYPIMDLADKVVINGGEISFMSMKNPQQVLTAQCFATKGAHNYTSTRISVTV 5265

HSP 14 Score: 87.0409 bits (214), Expect = 2.882e-21
Identity = 48/94 (51.06%), Postives = 59/94 (62.77%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K              NPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 4420 SNKEEYPIDKLAEK--------------NPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 4499

HSP 15 Score: 84.3445 bits (207), Expect = 2.520e-20
Identity = 43/71 (60.56%), Postives = 51/71 (71.83%), Query Frame = 2 
Query:   71 FISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 4194 FNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 4264

HSP 16 Score: 81.6481 bits (200), Expect = 2.291e-19
Identity = 54/113 (47.79%), Postives = 66/113 (58.41%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEI--SFISMKNPQQVLTAQCFATNGAQNFTSHSFLVD-----VSKAEF----PSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +D   Y IKD + + E+   EI  +F   +    +LT +        N T +  + D     +S   F    PSGVRFVVAGLDG  +FNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 3346 TDVTWYQIKD-SQEVELLKSEIKDNFNVYQKQPNILTVKILNMKKNSNMTLNCVVKDANGVMISSPVFLTQSPSGVRFVVAGLDGDSSFNPKQGSMDRKLQASIIDAKTGKDL 3457

HSP 17 Score: 78.1814 bits (191), Expect = 3.593e-18
Identity = 36/54 (66.67%), Postives = 42/54 (77.78%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLV 163
            S+K+EYPI  LA+K +  GGEI F+ MKNPQQ+LTAQCFATNG  NFTS S  V
Sbjct: 3470 SNKEEYPIDKLAEKVDQKGGEIKFMLMKNPQQILTAQCFATNGTHNFTSPSITV 3523

HSP 18 Score: 77.411 bits (189), Expect = 6.961e-18
Identity = 40/59 (67.80%), Postives = 42/59 (71.19%), Query Frame = 3 
Query:    3 RTNKNIQSKIWQTNLR*MEEKSHSFQ*RIHNKFSRLSALRQMGLRTLHLIPSLWTSVKQ 179
            RTNKNIQS+IWQT                 NKFSRLSALRQMGLRT+HLIPSLWTSVKQ
Sbjct: 4124 RTNKNIQSRIWQTKSDKWRRHLIYVNEESANKFSRLSALRQMGLRTIHLIPSLWTSVKQ 4182

HSP 19 Score: 63.1586 bits (152), Expect = 8.776e-13
Identity = 32/82 (39.02%), Postives = 46/82 (56.10%), Query Frame = 2 
Query:   17 YPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDR 262
            YPIKDLA+      G++SF+++KNP+QVL AQC   NG+ N  S  F + V + E      F+    +G LN   +   +DR
Sbjct: 3152 YPIKDLAENLIEKDGKLSFMTVKNPRQVLYAQCRVNNGSVNDVSKPFFIHVDEDELKRPKLFLKPTFEGELNAVKRLDCIDR 3233
[Close]
BLAST of SMED30031902 vs. Planmine SMEST
Match: SMESG000055070.1 (SMESG000055070.1)

HSP 1 Score: 186.808 bits (473), Expect = 3.329e-56
Identity = 90/102 (88.24%), Postives = 95/102 (93.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKAE P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 4982 ADKQEYPIKDLADKVGINGGDISFLSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 5083

HSP 2 Score: 186.808 bits (473), Expect = 3.964e-56
Identity = 90/102 (88.24%), Postives = 95/102 (93.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKAE P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 4526 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 4627

HSP 3 Score: 186.808 bits (473), Expect = 3.964e-56
Identity = 90/102 (88.24%), Postives = 95/102 (93.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKAE P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 4754 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 4855

HSP 4 Score: 184.496 bits (467), Expect = 2.206e-55
Identity = 89/102 (87.25%), Postives = 95/102 (93.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKAE P+GVRFVV GLD   +FNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 3548 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAESPAGVRFVVTGLDRDSSFNPKQGSMDRKLQASIIDAKTGKDL 3649

HSP 5 Score: 180.644 bits (457), Expect = 4.960e-54
Identity = 90/108 (83.33%), Postives = 95/108 (87.96%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEF------PSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKA F      P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 3776 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAVFLSIPESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 3883

HSP 6 Score: 180.644 bits (457), Expect = 4.960e-54
Identity = 90/108 (83.33%), Postives = 95/108 (87.96%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEF------PSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +DKQEYPIKDLADK  INGG+ISF+SMKNPQQVLTAQCFATNGAQN+TSHSFLVDVSKA F      P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 4306 ADKQEYPIKDLADKVGINGGDISFMSMKNPQQVLTAQCFATNGAQNYTSHSFLVDVSKAVFLSIPESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 4413

HSP 7 Score: 152.525 bits (384), Expect = 3.309e-44
Identity = 78/108 (72.22%), Postives = 85/108 (78.70%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKA------EFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA      E P+GVRFVV GLD   NFNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 3896 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKAVLLSIPESPAGVRFVVTGLDRDSNFNPKQGSMDRKLQASIIDAKTGKDL 4003

HSP 8 Score: 111.694 bits (278), Expect = 6.301e-30
Identity = 58/98 (59.18%), Postives = 70/98 (71.43%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAK 292
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I D K
Sbjct: 4016 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYIEDDK 4113

HSP 9 Score: 111.694 bits (278), Expect = 7.361e-30
Identity = 57/94 (60.64%), Postives = 68/94 (72.34%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KAE  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 5096 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKAETQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 5189

HSP 10 Score: 110.153 bits (274), Expect = 2.653e-29
Identity = 56/94 (59.57%), Postives = 68/94 (72.34%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 4640 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 4733

HSP 11 Score: 110.153 bits (274), Expect = 2.653e-29
Identity = 56/94 (59.57%), Postives = 68/94 (72.34%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K +  GGEI F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 4868 SNKEEYPIDKLAEKVDQKGGEIKFNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 4961

HSP 12 Score: 109.768 bits (273), Expect = 3.801e-29
Identity = 55/94 (58.51%), Postives = 68/94 (72.34%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K +  GGEI F+ MKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 3662 SNKEEYPIDKLAEKVDQKGGEIKFMLMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 3755

HSP 13 Score: 90.5077 bits (223), Expect = 1.876e-22
Identity = 43/56 (76.79%), Postives = 46/56 (82.14%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDV 169
            +DKQEYPI DLADK  INGGEISF+SMKNPQQVLTAQCFAT GA N+TS    V V
Sbjct: 5210 ADKQEYPIMDLADKVVINGGEISFMSMKNPQQVLTAQCFATKGAHNYTSTRISVTV 5265

HSP 14 Score: 87.0409 bits (214), Expect = 2.882e-21
Identity = 48/94 (51.06%), Postives = 59/94 (62.77%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            S+K+EYPI  LA+K              NPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 4426 SNKEEYPIDKLAEK--------------NPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 4505

HSP 15 Score: 84.3445 bits (207), Expect = 2.520e-20
Identity = 43/71 (60.56%), Postives = 51/71 (71.83%), Query Frame = 2 
Query:   71 FISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSG-VRFVVAGLDGHLNFNPKQGSMDRKLQASI 280
            F SMKNPQQ+LTAQCFATNG  NFTS S  VDV+KA+  +G     V+GL G  NF PK GS+D+KL+  I
Sbjct: 4200 FNSMKNPQQILTAQCFATNGTHNFTSPSITVDVNKADTQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYI 4270

HSP 16 Score: 81.6481 bits (200), Expect = 2.291e-19
Identity = 54/113 (47.79%), Postives = 66/113 (58.41%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEI--SFISMKNPQQVLTAQCFATNGAQNFTSHSFLVD-----VSKAEF----PSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGKDL 307
            +D   Y IKD + + E+   EI  +F   +    +LT +        N T +  + D     +S   F    PSGVRFVVAGLDG  +FNPKQGSMDRKLQASIIDAKTGKDL
Sbjct: 3346 TDVTWYQIKD-SQEVELLKSEIKDNFNVYQKQPNILTVKILNMKKNSNMTLNCVVKDANGVMISSPVFLTQSPSGVRFVVAGLDGDSSFNPKQGSMDRKLQASIIDAKTGKDL 3457

HSP 17 Score: 78.1814 bits (191), Expect = 3.593e-18
Identity = 36/54 (66.67%), Postives = 42/54 (77.78%), Query Frame = 2 
Query:    2 SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLV 163
            S+K+EYPI  LA+K +  GGEI F+ MKNPQQ+LTAQCFATNG  NFTS S  V
Sbjct: 3470 SNKEEYPIDKLAEKVDQKGGEIKFMLMKNPQQILTAQCFATNGTHNFTSPSITV 3523

HSP 18 Score: 77.411 bits (189), Expect = 6.961e-18
Identity = 40/59 (67.80%), Postives = 42/59 (71.19%), Query Frame = 3 
Query:    3 RTNKNIQSKIWQTNLR*MEEKSHSFQ*RIHNKFSRLSALRQMGLRTLHLIPSLWTSVKQ 179
            RTNKNIQS+IWQT                 NKFSRLSALRQMGLRT+HLIPSLWTSVKQ
Sbjct: 4130 RTNKNIQSRIWQTKSDKWRRHLIYVNEESANKFSRLSALRQMGLRTIHLIPSLWTSVKQ 4188

HSP 19 Score: 63.1586 bits (152), Expect = 8.776e-13
Identity = 32/82 (39.02%), Postives = 46/82 (56.10%), Query Frame = 2 
Query:   17 YPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSHSFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDR 262
            YPIKDLA+      G++SF+++KNP+QVL AQC   NG+ N  S  F + V + E      F+    +G LN   +   +DR
Sbjct: 3152 YPIKDLAENLIEKDGKLSFMTVKNPRQVLYAQCRVNNGSVNDVSKPFFIHVDEDELKRPKLFLKPTFEGELNAVKRLDCIDR 3233
The following BLAST results are available for this feature:
BLAST of SMED30031902 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031902 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031902 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031902 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031902 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031902 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031902 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031902 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031902 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031902 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031902 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031902 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031902 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031902 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000055070.13.202e-5688.24SMESG000055070.1[more]
SMESG000055070.13.265e-5688.24SMESG000055070.1[more]
SMESG000055070.13.329e-5688.24SMESG000055070.1[more]
SMESG000055070.13.329e-5688.24SMESG000055070.1[more]
SMESG000055070.13.329e-5688.24SMESG000055070.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031902 ID=SMED30031902|Name=SMED30031902|organism=Schmidtea mediterranea sexual|type=transcript|length=307bp
CTCGGACAAACAAGAATATCCAATCAAAGATTTGGCAGACAAATTTGAGA
TAAATGGAGGAGAAATCTCATTCATTTCAATGAAGAATCCACAACAAGTT
CTCACGGCTCAGTGCTTTGCGACAAATGGGGCTCAGAACTTTACATCTCA
TTCCTTCCTTGTGGACGTCAGTAAAGCAGAATTTCCATCCGGAGTGAGAT
TTGTGGTTGCCGGTCTTGATGGGCACTTGAATTTCAATCCCAAACAAGGC
TCAATGGACAGAAAACTGCAAGCTTCAATAATAGACGCGAAAACTGGAAA
AGATCTG
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protein sequence of SMED30031902-orf-1

>SMED30031902-orf-1 ID=SMED30031902-orf-1|Name=SMED30031902-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=102bp
SDKQEYPIKDLADKFEINGGEISFISMKNPQQVLTAQCFATNGAQNFTSH
SFLVDVSKAEFPSGVRFVVAGLDGHLNFNPKQGSMDRKLQASIIDAKTGK
DL
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell