SMED30031829

Overview
NameSMED30031829
Smed IDSMED30031829
Length (bp)1416
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30031829 (SMED30031829) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30031829 (SMED30031829) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30031829 (SMED30031829) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30031829 (SMED30031829) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30031829

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 15

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30031829h1SMcG0018207 Contig2191GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30031829h1SMcG0018207 Contig2191GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
Smed sexual biotypeSMED30031829h1SMcG0018207 Contig38713newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031829h1SMcG0018207 Contig38713uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30031829h1SMcG0018207 dd_Smed_v4_502_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30031829h1SMcG0018207 dd_Smed_v4_502_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30031829h1SMcG0018207 dd_Smed_v4_502_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30031829h1SMcG0018207 dd_Smed_v4_502_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30031829h1SMcG0018207 dd_Smed_v4_502_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30031829h1SMcG0018207 dd_Smed_v4_502_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031829h1SMcG0018207 dd_Smed_v4_502_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30031829h1SMcG0018207 dd_Smed_v4_502_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30031829h1SMcG0018207 dd_Smed_v6_502_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031829h1SMcG0018207 dd_Smed_v6_502_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
glial cellSMED30031829h1SMcG0018207 dd_Smed_v6_502_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30031829 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000140:150135..151546 +6885
v31.001182:28831..38092 +3582
v31.002883:7184..14223 -3446
Homology
[Close]
BLAST of SMED30031829 vs. Planmine SMEST
Match: SMESG000028861.1 (SMESG000028861.1)

HSP 1 Score: 292.352 bits (747), Expect = 8.127e-98
Identity = 179/186 (96.24%), Postives = 182/186 (97.85%), Query Frame = 2 
Query:  797 MENCECLICLETDNKLLMSIGGCNHKCCIICGLESMRNTMTCPLDRXXXXXXXXXXXXXXXXXXXXXXXXXXXDENLTFEMVQRAILSXXXXXXXXXXXQIYTLDPYLIYIGPFVLNRNKFSREMIENYLNKFGPINDVIVKPNSAYAMFASSQHGMRAKYERNKDLFYGESVTIEHFHPDTWEYL 1354
            MENCECLICLETDNKLLMSIGGCNHKCCIICGLESMRNTMTCPLDREQITEITIYENTN IEKEKLKIKSLNSDENLTFEMVQRAILSIENIENR EIEQIYTLDPYLIYIGPFVLNRNK SREMIENYLN+FGPINDVIVKPNSAYAMF+SSQHGMRAKYERNK+LFYGESVTIE FHPDTWEYL
Sbjct:    1 MENCECLICLETDNKLLMSIGGCNHKCCIICGLESMRNTMTCPLDREQITEITIYENTNGIEKEKLKIKSLNSDENLTFEMVQRAILSIENIENRSEIEQIYTLDPYLIYIGPFVLNRNKVSREMIENYLNEFGPINDVIVKPNSAYAMFSSSQHGMRAKYERNKELFYGESVTIESFHPDTWEYL 186
[Close]
BLAST of SMED30031829 vs. Planmine SMEST
Match: SMESG000038655.1 (SMESG000038655.1)

HSP 1 Score: 289.656 bits (740), Expect = 8.284e-97
Identity = 178/186 (95.70%), Postives = 183/186 (98.39%), Query Frame = 2 
Query:  797 MENCECLICLETDNKLLMSIGGCNHKCCIICGLESMRNTMTCPLDRXXXXXXXXXXXXXXXXXXXXXXXXXXXDENLTFEMVQRAILSXXXXXXXXXXXQIYTLDPYLIYIGPFVLNRNKFSREMIENYLNKFGPINDVIVKPNSAYAMFASSQHGMRAKYERNKDLFYGESVTIEHFHPDTWEYL 1354
            MENCECLICLETDNKLLMSIGGCNHKCCIICGLESMRNTMTCPLDREQITEITIYENTNEIEKEKLKIKSLNSDENLTFEMVQRAILSIENIENR EIEQIYTLDPYLIYIGPFVLNRNK S+EMIENYLN+FGPINDVIVKPNSAYAMFASSQHGMRAKYERNK+LF+GESVTIE FHP+TWEYL
Sbjct:    1 MENCECLICLETDNKLLMSIGGCNHKCCIICGLESMRNTMTCPLDREQITEITIYENTNEIEKEKLKIKSLNSDENLTFEMVQRAILSIENIENRSEIEQIYTLDPYLIYIGPFVLNRNKVSKEMIENYLNEFGPINDVIVKPNSAYAMFASSQHGMRAKYERNKELFHGESVTIESFHPETWEYL 186
[Close]
BLAST of SMED30031829 vs. Planmine SMEST
Match: SMESG000028695.1 (SMESG000028695.1)

HSP 1 Score: 280.411 bits (716), Expect = 3.567e-93
Identity = 172/186 (92.47%), Postives = 177/186 (95.16%), Query Frame = 2 
Query:  797 MENCECLICLETDNKLLMSIGGCNHKCCIICGLESMRNTMTCPLDRXXXXXXXXXXXXXXXXXXXXXXXXXXXDENLTFEMVQRAILSXXXXXXXXXXXQIYTLDPYLIYIGPFVLNRNKFSREMIENYLNKFGPINDVIVKPNSAYAMFASSQHGMRAKYERNKDLFYGESVTIEHFHPDTWEYL 1354
            MENCECLICLETDNKLLMSIGGCNHKCCIICGLE MRNTMTCPLDREQITEITIYENTN IE+EKLKIKS   DENLTFEMVQRAILSIENIENR EIEQI+TLDPYLIYIGPFVLNRNK S+EMIENYLNKFGPINDVIVKPNSAYAMFASSQHGMRAKYERNK+LFYGESVTI  FHP+TWEYL
Sbjct:    1 MENCECLICLETDNKLLMSIGGCNHKCCIICGLELMRNTMTCPLDREQITEITIYENTNGIEREKLKIKSS--DENLTFEMVQRAILSIENIENRSEIEQIHTLDPYLIYIGPFVLNRNKVSKEMIENYLNKFGPINDVIVKPNSAYAMFASSQHGMRAKYERNKELFYGESVTIGSFHPNTWEYL 184
[Close]
BLAST of SMED30031829 vs. Planmine SMEST
Match: SMESG000028540.1 (SMESG000028540.1)

HSP 1 Score: 221.09 bits (562), Expect = 1.457e-70
Identity = 145/151 (96.03%), Postives = 147/151 (97.35%), Query Frame = 2 
Query:  902 MRNTMTCPLDRXXXXXXXXXXXXXXXXXXXXXXXXXXXDENLTFEMVQRAILSXXXXXXXXXXXQIYTLDPYLIYIGPFVLNRNKFSREMIENYLNKFGPINDVIVKPNSAYAMFASSQHGMRAKYERNKDLFYGESVTIEHFHPDTWEYL 1354
            MRNTMTCPLDREQITEITIYENTNEIEKEKLKIKSLN DENLTFEMVQRAILSIENIENR EIEQIYTLDPYLIYIGPFVLNRNK SREMIENYLNKFGPINDVIVKPNSAYAMFASSQHGMRAKYERNK+LF+GESVTIE FHPDTWEYL
Sbjct:    1 MRNTMTCPLDREQITEITIYENTNEIEKEKLKIKSLNPDENLTFEMVQRAILSIENIENRSEIEQIYTLDPYLIYIGPFVLNRNKVSREMIENYLNKFGPINDVIVKPNSAYAMFASSQHGMRAKYERNKELFHGESVTIESFHPDTWEYL 151
[Close]
BLAST of SMED30031829 vs. Planmine SMEST
Match: SMESG000038688.1 (SMESG000038688.1)

HSP 1 Score: 220.705 bits (561), Expect = 1.947e-70
Identity = 145/151 (96.03%), Postives = 148/151 (98.01%), Query Frame = 2 
Query:  902 MRNTMTCPLDRXXXXXXXXXXXXXXXXXXXXXXXXXXXDENLTFEMVQRAILSXXXXXXXXXXXQIYTLDPYLIYIGPFVLNRNKFSREMIENYLNKFGPINDVIVKPNSAYAMFASSQHGMRAKYERNKDLFYGESVTIEHFHPDTWEYL 1354
            MRNTMTCPLDREQITEITIYENTNEIEKEKLKIKSLNSDENLTFEMVQRAILSIENIENR EIEQIYTLDPYLIYIGPFVLNRNK SREMIENYLN+FGPINDVIVKPNSAYAMFASSQHGMRAKYERNK+LFYGESVTIE FHPDTWE+L
Sbjct:    1 MRNTMTCPLDREQITEITIYENTNEIEKEKLKIKSLNSDENLTFEMVQRAILSIENIENRSEIEQIYTLDPYLIYIGPFVLNRNKVSREMIENYLNEFGPINDVIVKPNSAYAMFASSQHGMRAKYERNKELFYGESVTIESFHPDTWEHL 151
The following BLAST results are available for this feature:
BLAST of SMED30031829 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30031829 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30031829 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30031829 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30031829 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30031829 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30031829 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30031829 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30031829 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30031829 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30031829 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30031829 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30031829 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30031829 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000028861.18.127e-9896.24SMESG000028861.1[more]
SMESG000038655.18.284e-9795.70SMESG000038655.1[more]
SMESG000028695.13.567e-9392.47SMESG000028695.1[more]
SMESG000028540.11.457e-7096.03SMESG000028540.1[more]
SMESG000038688.11.947e-7096.03SMESG000038688.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031829 ID=SMED30031829|Name=SMED30031829|organism=Schmidtea mediterranea sexual|type=transcript|length=1416bp
GTTGCATTCACTAGGTGTTTGGTATGTTTTGATATTTACACTTTGGACTA
GTAATTGGATTTCGGTCCAATGATATTCATTCGTCATTCATTTAGCCGTT
GGATATTGTTCACGTTGCCAGCGCATGTTTCATTCATAATTTATAGGCAT
CATGCAATCGACTATTTTTACGAAAATTTATAAAAATTAATCATCTAATT
AAGTTTAATTTAATTTTAAAAACTTGAAACACTATTAAAGCACATAGATA
TGTAACACCAACCCTTTCTTTAAATTATATTTTATTATTTATAAAATAAA
AAAGGACGAACGATCGGCGTAAAGTAATTACCCTTTACTCTTATATATTC
ATACAGAGTGTGCAGTATTGGTACAACTGTGGCAGTATTCTCAATATTAA
TAAATTACATTTTAGCTTCATCCTTTACACTGGCCGTGTTTTTACAACCC
ATATACTAGAAACCTGAACCATCGGGTTACAAGGGATTATTTCAGTGTCC
CGGTACGGAGATCCTCTCATCAAAGGTGTTTGAAAGATATTATAACAACT
TATCTTTATTATGGCATATTTTTCAATCAGTAACCAACATCAGTTTAAGA
AGACCTGCATAAATATTTTAAAAATTGCAAGCACTTAACGATATCATTAA
AACCGCAATTTTTGAATATCAATAGCTTTAAAAAATGTAACTATAATCTA
TTAATTTCGACGAATCAAAATTGAAAGAATTGGAATTTATTTATAATATA
TCTTTATTTATTATTTTTTAAAGATATAAAGTTAAAAACAAATAAAATGG
AAAATTGTGAATGTTTAATCTGTTTAGAAACTGACAATAAATTACTAATG
TCTATTGGGGGGTGCAATCATAAGTGCTGTATAATTTGTGGGCTTGAATC
AATGAGAAACACCATGACTTGTCCTCTTGATAGAGAGCAGATCACAGAAA
TTACAATATATGAAAATACAAATGAAATAGAAAAAGAAAAATTAAAAATA
AAATCTTTAAATTCAGATGAAAATCTCACATTTGAAATGGTGCAAAGAGC
GATTTTGTCGATTGAAAATATTGAAAATAGGATTGAAATCGAGCAAATTT
ATACACTTGATCCATATTTGATCTACATAGGACCATTTGTTTTAAATCGG
AATAAATTCAGTAGGGAAATGATTGAAAATTATTTGAACAAATTCGGACC
GATAAATGATGTAATAGTTAAACCGAATTCTGCCTACGCCATGTTTGCCA
GTTCCCAGCATGGAATGAGAGCGAAATATGAAAGAAATAAAGATTTGTTT
TATGGGGAATCTGTAACAATTGAACATTTTCATCCAGACACTTGGGAGTA
TTTATAAAATTTTAATGCAAACTTTATTCGTTTAAGTAGCGCTTTCTAAT
AATGCAATCATTGTCA
back to top

protein sequence of SMED30031829-orf-1

>SMED30031829-orf-1 ID=SMED30031829-orf-1|Name=SMED30031829-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=187bp
MENCECLICLETDNKLLMSIGGCNHKCCIICGLESMRNTMTCPLDREQIT
EITIYENTNEIEKEKLKIKSLNSDENLTFEMVQRAILSIENIENRIEIEQ
IYTLDPYLIYIGPFVLNRNKFSREMIENYLNKFGPINDVIVKPNSAYAMF
ASSQHGMRAKYERNKDLFYGESVTIEHFHPDTWEYL*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR035979RBD_domain_sf
IPR013083Znf_RING/FYVE/PHD
IPR012677Nucleotide-bd_a/b_plait_sf
IPR001841Znf_RING
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
GO:0000166nucleotide binding
GO:0005515protein binding
GO:0008270zinc ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 1..98
e-value: 5.7E-6
score: 28.0
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 6..46
score: 9.244
NoneNo IPR availableCDDcd00590RRM_SFcoord: 107..174
e-value: 7.49781E-4
score: 34.5881
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 2..53
IPR035979RNA-binding domain superfamilySUPERFAMILYSSF54928RNA-binding domain, RBDcoord: 114..179