Mth938 domain-containing protein

Overview
NameMth938 domain-containing protein
Smed IDSMED30031800
Length (bp)532
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Mth938 domain-containing protein (SMED30031800) t-SNE clustered cells

Violin plots show distribution of expression levels for Mth938 domain-containing protein (SMED30031800) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Mth938 domain-containing protein (SMED30031800) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Mth938 domain-containing protein (SMED30031800) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30031800

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30031800h1SMcG0010405 Contig42364uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031800h1SMcG0010405 Contig42364newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30031800h1SMcG0010405 dd_Smed_v4_2203_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30031800h1SMcG0010405 dd_Smed_v4_2203_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031800h1SMcG0010405 dd_Smed_v4_2203_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Mth938 domain-containing protein vs. Ensembl Human
Match: AAMDC (adipogenesis associated Mth938 domain containing [Source:HGNC Symbol;Acc:HGNC:30205])

HSP 1 Score: 134.42 bits (337), Expect = 2.895e-40
Identity = 63/127 (49.61%), Postives = 85/127 (66.93%), Query Frame = 1 
Query:  100 YVMKPGIISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
            ++M    I+ +SWG++ +  +N     YKD KVWPGGSR WDW ET T H PG+Q  DV E+++KGV+ +V+ +GM   L VP  TV+Y+K  G+ V V  T +AV  YN LV+Q  RVGG+FHSTC
Sbjct:   18 FLMTSPEIASLSWGQMKVKGSNT---TYKDCKVWPGGSRTWDWRETGTEHSPGVQPADVKEVVEKGVQTLVIGRGMSEALKVPSSTVEYLKKHGIDVRVLQTEQAVKEYNALVAQGVRVGGVFHSTC 141
[Close]
BLAST of Mth938 domain-containing protein vs. Ensembl Human
Match: AAMDC (adipogenesis associated Mth938 domain containing [Source:HGNC Symbol;Acc:HGNC:30205])

HSP 1 Score: 133.265 bits (334), Expect = 5.650e-40
Identity = 62/120 (51.67%), Postives = 82/120 (68.33%), Query Frame = 1 
Query:  121 ISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
            I+ +SWG++ +  +N     YKD KVWPGGSR WDW ET T H PG+Q  DV E+++KGV+ +V+ +GM   L VP  TV+Y+K  G+ V V  T +AV  YN LV+Q  RVGG+FHSTC
Sbjct:    6 IASLSWGQMKVKGSNT---TYKDCKVWPGGSRTWDWRETGTEHSPGVQPADVKEVVEKGVQTLVIGRGMSEALKVPSSTVEYLKKHGIDVRVLQTEQAVKEYNALVAQGVRVGGVFHSTC 122
[Close]
BLAST of Mth938 domain-containing protein vs. Ensembl Human
Match: AAMDC (adipogenesis associated Mth938 domain containing [Source:HGNC Symbol;Acc:HGNC:30205])

HSP 1 Score: 133.265 bits (334), Expect = 5.650e-40
Identity = 62/120 (51.67%), Postives = 82/120 (68.33%), Query Frame = 1 
Query:  121 ISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
            I+ +SWG++ +  +N     YKD KVWPGGSR WDW ET T H PG+Q  DV E+++KGV+ +V+ +GM   L VP  TV+Y+K  G+ V V  T +AV  YN LV+Q  RVGG+FHSTC
Sbjct:    6 IASLSWGQMKVKGSNT---TYKDCKVWPGGSRTWDWRETGTEHSPGVQPADVKEVVEKGVQTLVIGRGMSEALKVPSSTVEYLKKHGIDVRVLQTEQAVKEYNALVAQGVRVGGVFHSTC 122
[Close]
BLAST of Mth938 domain-containing protein vs. Ensembl Human
Match: AAMDC (adipogenesis associated Mth938 domain containing [Source:HGNC Symbol;Acc:HGNC:30205])

HSP 1 Score: 113.235 bits (282), Expect = 1.378e-31
Identity = 62/166 (37.35%), Postives = 83/166 (50.00%), Query Frame = 1 
Query:  121 ISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSET----------------------------------------------NTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
            I+ +SWG++ +  +N     YKD KVWPGGSR WDW ET                                              +T H PG+Q  DV E+++KGV+ +V+ +GM   L VP  TV+Y+K  G+ V V  T +AV  YN LV+Q  RVGG+FHSTC
Sbjct:    6 IASLSWGQMKVKGSNT---TYKDCKVWPGGSRTWDWRETGTESLTLLPRLECSGAISAHCNLHLPGSCHSPASASQVAGTTGACHHTQHSPGVQPADVKEVVEKGVQTLVIGRGMSEALKVPSSTVEYLKKHGIDVRVLQTEQAVKEYNALVAQGVRVGGVFHSTC 168
[Close]
BLAST of Mth938 domain-containing protein vs. Ensembl Human
Match: AAMDC (adipogenesis associated Mth938 domain containing [Source:HGNC Symbol;Acc:HGNC:30205])

HSP 1 Score: 84.7297 bits (208), Expect = 1.927e-21
Identity = 48/120 (40.00%), Postives = 60/120 (50.00%), Query Frame = 1 
Query:  121 ISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
            I+ +SWG++ +  +N     YKD KVWPGGSR WDW ET T                                 VP  TV+Y+K  G+ V V  T +AV  YN LV+Q  RVGG+FHSTC
Sbjct:    6 IASLSWGQMKVKGSNT---TYKDCKVWPGGSRTWDWRETGTE--------------------------------VPSSTVEYLKKHGIDVRVLQTEQAVKEYNALVAQGVRVGGVFHSTC 90
[Close]
BLAST of Mth938 domain-containing protein vs. Ensembl Zebrafish
Match: aamdc (adipogenesis associated, Mth938 domain containing [Source:NCBI gene;Acc:553802])

HSP 1 Score: 131.339 bits (329), Expect = 1.671e-39
Identity = 61/120 (50.83%), Postives = 82/120 (68.33%), Query Frame = 1 
Query:  121 ISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
            I+ +SWG + +   +     YKD KVWPGGSR WDW ET T+H PG+Q  D++EIL+KGV+ +V+ +GM   L VP  T+DYVK +GV V V  T +AV  YN L  + ++VGG+FHSTC
Sbjct:    6 IASLSWGHMKVKGCSTS---YKDCKVWPGGSRAWDWRETGTNHHPGVQPSDLEEILRKGVQTLVIGRGMSEALQVPPSTLDYVKKQGVDVQVFQTEQAVREYNALAGKGEKVGGVFHSTC 122
[Close]
BLAST of Mth938 domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000009445.1 (adipogenesis associated, Mth938 domain containing [Source:NCBI gene;Acc:100379953])

HSP 1 Score: 58.9214 bits (141), Expect = 6.747e-12
Identity = 28/52 (53.85%), Postives = 35/52 (67.31%), Query Frame = 1 
Query:  325 MQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
            M   L VP  T++Y+K +GV  +V  T +AV  YN LVSQ  RVGG+FHSTC
Sbjct:    1 MTETLQVPAATLEYIKSQGVDPLVFQTEKAVKEYNSLVSQGARVGGVFHSTC 52
[Close]
BLAST of Mth938 domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000001265.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:KV461231.1:13971:14459:1 gene:ENSXETG00000006213.1 transcript:ENSXETT00000001265.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 47.7506 bits (112), Expect = 1.176e-7
Identity = 24/42 (57.14%), Postives = 27/42 (64.29%), Query Frame = 1 
Query:  121 ISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTH 246
            IS+ISWG + +        VYKD KVWPGGSR WDW ET T 
Sbjct:    6 ISKISWGSMKVGGQ-----VYKDCKVWPGGSRAWDWRETGTE 42
[Close]
BLAST of Mth938 domain-containing protein vs. Ensembl Mouse
Match: Aamdc (adipogenesis associated Mth938 domain containing [Source:MGI Symbol;Acc:MGI:1913523])

HSP 1 Score: 131.339 bits (329), Expect = 2.845e-39
Identity = 61/131 (46.56%), Postives = 84/131 (64.12%), Query Frame = 1 
Query:   88 FTSAYVMKPGIISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
             ++  +M    I+ +SWG+   +      + YKD KVWPGGSR WDW ET T H PG+Q  DV E+ +KGV+ +V+ +GM   L VP  TV+Y++ +G+ V V  T +AV  YN LV+Q  RVGG+FHSTC
Sbjct:   12 ISAQLLMASPKIASLSWGQ---MKVQGSTLTYKDCKVWPGGSRAWDWRETGTEHSPGVQPADVKEVAEKGVQTLVIGRGMSEALKVPPSTVEYLEKQGIDVRVLQTEQAVKEYNALVAQGVRVGGVFHSTC 139
[Close]
BLAST of Mth938 domain-containing protein vs. Ensembl Mouse
Match: Aamdc (adipogenesis associated Mth938 domain containing [Source:MGI Symbol;Acc:MGI:1913523])

HSP 1 Score: 131.339 bits (329), Expect = 4.261e-39
Identity = 61/131 (46.56%), Postives = 84/131 (64.12%), Query Frame = 1 
Query:   88 FTSAYVMKPGIISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
             ++  +M    I+ +SWG+   +      + YKD KVWPGGSR WDW ET T H PG+Q  DV E+ +KGV+ +V+ +GM   L VP  TV+Y++ +G+ V V  T +AV  YN LV+Q  RVGG+FHSTC
Sbjct:   23 ISAQLLMASPKIASLSWGQ---MKVQGSTLTYKDCKVWPGGSRAWDWRETGTEHSPGVQPADVKEVAEKGVQTLVIGRGMSEALKVPPSTVEYLEKQGIDVRVLQTEQAVKEYNALVAQGVRVGGVFHSTC 150
[Close]
BLAST of Mth938 domain-containing protein vs. Ensembl Mouse
Match: Aamdc (adipogenesis associated Mth938 domain containing [Source:MGI Symbol;Acc:MGI:1913523])

HSP 1 Score: 131.339 bits (329), Expect = 4.261e-39
Identity = 61/131 (46.56%), Postives = 84/131 (64.12%), Query Frame = 1 
Query:   88 FTSAYVMKPGIISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
             ++  +M    I+ +SWG+   +      + YKD KVWPGGSR WDW ET T H PG+Q  DV E+ +KGV+ +V+ +GM   L VP  TV+Y++ +G+ V V  T +AV  YN LV+Q  RVGG+FHSTC
Sbjct:   23 ISAQLLMASPKIASLSWGQ---MKVQGSTLTYKDCKVWPGGSRAWDWRETGTEHSPGVQPADVKEVAEKGVQTLVIGRGMSEALKVPPSTVEYLEKQGIDVRVLQTEQAVKEYNALVAQGVRVGGVFHSTC 150
[Close]
BLAST of Mth938 domain-containing protein vs. Ensembl Mouse
Match: Aamdc (adipogenesis associated Mth938 domain containing [Source:MGI Symbol;Acc:MGI:1913523])

HSP 1 Score: 130.183 bits (326), Expect = 5.075e-39
Identity = 60/120 (50.00%), Postives = 80/120 (66.67%), Query Frame = 1 
Query:  121 ISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
            I+ +SWG+   +      + YKD KVWPGGSR WDW ET T H PG+Q  DV E+ +KGV+ +V+ +GM   L VP  TV+Y++ +G+ V V  T +AV  YN LV+Q  RVGG+FHSTC
Sbjct:    6 IASLSWGQ---MKVQGSTLTYKDCKVWPGGSRAWDWRETGTEHSPGVQPADVKEVAEKGVQTLVIGRGMSEALKVPPSTVEYLEKQGIDVRVLQTEQAVKEYNALVAQGVRVGGVFHSTC 122
[Close]
BLAST of Mth938 domain-containing protein vs. Ensembl Mouse
Match: Aamdc (adipogenesis associated Mth938 domain containing [Source:MGI Symbol;Acc:MGI:1913523])

HSP 1 Score: 98.2117 bits (243), Expect = 1.952e-26
Identity = 46/109 (42.20%), Postives = 67/109 (61.47%), Query Frame = 1 
Query:   88 FTSAYVMKPGIISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREA 414
             ++  +M    I+ +SWG+   +      + YKD KVWPGGSR WDW ET T H PG+Q  DV E+ +KGV+ +V+ +GM   L VP  TV+Y++ +G+ V V  T +A
Sbjct:   23 ISAQLLMASPKIASLSWGQ---MKVQGSTLTYKDCKVWPGGSRAWDWRETGTEHSPGVQPADVKEVAEKGVQTLVIGRGMSEALKVPPSTVEYLEKQGIDVRVLQTEQA 128
[Close]
BLAST of Mth938 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q32PA8|AAMDC_BOVIN (Mth938 domain-containing protein OS=Bos taurus OX=9913 GN=AAMDC PE=2 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 2.206e-39
Identity = 61/120 (50.83%), Postives = 84/120 (70.00%), Query Frame = 1 
Query:  121 ISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
            I+ +SWG++ +  +     +YKD KVWPGGSR+WDW ET T H PG+Q  DV+E+++KGV+ +V+ +GM   L VP  TV+Y+K KG+ V V  T +AV  YN L +Q  RVGG+FHSTC
Sbjct:    6 IASLSWGQMKVQGSTK---IYKDCKVWPGGSRDWDWRETGTEHSPGVQPADVEEVVEKGVQILVIGRGMSEALKVPPSTVEYLKKKGIDVRVLQTEQAVKEYNALATQGIRVGGVFHSTC 122
[Close]
BLAST of Mth938 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9H7C9|AAMDC_HUMAN (Mth938 domain-containing protein OS=Homo sapiens OX=9606 GN=AAMDC PE=1 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 2.713e-39
Identity = 62/120 (51.67%), Postives = 82/120 (68.33%), Query Frame = 1 
Query:  121 ISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
            I+ +SWG++ +  +N     YKD KVWPGGSR WDW ET T H PG+Q  DV E+++KGV+ +V+ +GM   L VP  TV+Y+K  G+ V V  T +AV  YN LV+Q  RVGG+FHSTC
Sbjct:    6 IASLSWGQMKVKGSNT---TYKDCKVWPGGSRTWDWRETGTEHSPGVQPADVKEVVEKGVQTLVIGRGMSEALKVPSSTVEYLKKHGIDVRVLQTEQAVKEYNALVAQGVRVGGVFHSTC 122
[Close]
BLAST of Mth938 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q502H1|AAMDC_DANRE (Mth938 domain-containing protein OS=Danio rerio OX=7955 GN=aamdc PE=2 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 1.261e-38
Identity = 61/120 (50.83%), Postives = 82/120 (68.33%), Query Frame = 1 
Query:  121 ISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
            I+ +SWG + +   +     YKD KVWPGGSR WDW ET T+H PG+Q  D++EIL+KGV+ +V+ +GM   L VP  T+DYVK +GV V V  T +AV  YN L  + ++VGG+FHSTC
Sbjct:    6 IASLSWGHMKVKGCSTS---YKDCKVWPGGSRAWDWRETGTNHHPGVQPSDLEEILRKGVQTLVIGRGMSEALQVPPSTLDYVKKQGVDVQVFQTEQAVREYNALAGKGEKVGGVFHSTC 122
[Close]
BLAST of Mth938 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q8R0P4|AAMDC_MOUSE (Mth938 domain-containing protein OS=Mus musculus OX=10090 GN=Aamdc PE=1 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 3.550e-38
Identity = 60/120 (50.00%), Postives = 80/120 (66.67%), Query Frame = 1 
Query:  121 ISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
            I+ +SWG+   +      + YKD KVWPGGSR WDW ET T H PG+Q  DV E+ +KGV+ +V+ +GM   L VP  TV+Y++ +G+ V V  T +AV  YN LV+Q  RVGG+FHSTC
Sbjct:    6 IASLSWGQ---MKVQGSTLTYKDCKVWPGGSRAWDWRETGTEHSPGVQPADVKEVAEKGVQTLVIGRGMSEALKVPPSTVEYLEKQGIDVRVLQTEQAVKEYNALVAQGVRVGGVFHSTC 122
[Close]
BLAST of Mth938 domain-containing protein vs. TrEMBL
Match: F7C200 (Uncharacterized protein OS=Monodelphis domestica OX=13616 GN=AAMDC PE=4 SV=2)

HSP 1 Score: 140.198 bits (352), Expect = 2.048e-39
Identity = 64/126 (50.79%), Postives = 85/126 (67.46%), Query Frame = 1 
Query:  103 VMKPGIISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
            +M    I+ +SWG++ + D +     YKD KVWPGGSR+WDW ET T H PG+Q  DV+E+ KKGV+ +V+  GM   L VP  TVDY+K +G+ V+V  T +AV  YN L +Q  RVGG+FHSTC
Sbjct:   14 IMSSPKIASLSWGQMKVQDCST---TYKDCKVWPGGSRSWDWRETGTEHSPGVQPADVEEVAKKGVQTLVIGHGMSEALKVPASTVDYLKKQGINVLVLQTEQAVKEYNALAAQGVRVGGVFHSTC 136
[Close]
BLAST of Mth938 domain-containing protein vs. TrEMBL
Match: A0A5F8G890 (Uncharacterized protein OS=Monodelphis domestica OX=13616 GN=AAMDC PE=4 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 2.962e-39
Identity = 63/120 (52.50%), Postives = 83/120 (69.17%), Query Frame = 1 
Query:  121 ISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
            I+ +SWG++ + D +     YKD KVWPGGSR+WDW ET T H PG+Q  DV+E+ KKGV+ +V+  GM   L VP  TVDY+K +G+ V+V  T +AV  YN L +Q  RVGG+FHSTC
Sbjct:    6 IASLSWGQMKVQDCST---TYKDCKVWPGGSRSWDWRETGTEHSPGVQPADVEEVAKKGVQTLVIGHGMSEALKVPASTVDYLKKQGINVLVLQTEQAVKEYNALAAQGVRVGGVFHSTC 122
[Close]
BLAST of Mth938 domain-containing protein vs. TrEMBL
Match: A0A4X2LA17 (Uncharacterized protein OS=Vombatus ursinus OX=29139 GN=AAMDC PE=4 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 7.166e-39
Identity = 62/120 (51.67%), Postives = 84/120 (70.00%), Query Frame = 1 
Query:  121 ISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
            I+ +SWG++ + D +     YKD KVWPGGSR+WDW ET T H PG+Q  DV+E+ KKGV+ +V+ +GM   L VP  TVDY+K +G+ V+V  T +AV  YN L +Q  +VGG+FHSTC
Sbjct:    6 IASLSWGQMKVQDCST---TYKDCKVWPGGSRSWDWRETGTEHSPGVQPADVEEVAKKGVQTLVIGRGMSEALKVPASTVDYLKRQGINVLVLQTEQAVKEYNALAAQGVKVGGVFHSTC 122
[Close]
BLAST of Mth938 domain-containing protein vs. TrEMBL
Match: A0A4X1U924 (Uncharacterized protein OS=Sus scrofa OX=9823 GN=AAMDC PE=4 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 1.033e-38
Identity = 63/127 (49.61%), Postives = 88/127 (69.29%), Query Frame = 1 
Query:  100 YVMKPGIISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
            ++M    I+ +SWG++ +  +     +YKD KVWPGGSR WDW ET T H PG+Q  DV+E+++KGV+ +V+ +GM   L VP+ TV+Y+K KG+ V V  T +AV  YN LV+Q  RVGG+FHSTC
Sbjct:    6 FLMSSPEIASLSWGQMKVQGSTK---IYKDCKVWPGGSRAWDWRETGTEHSPGVQPADVEEVVEKGVQILVIGRGMSEALKVPVSTVEYLKKKGIDVRVLQTEQAVKEYNALVTQGLRVGGVFHSTC 129
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BLAST of Mth938 domain-containing protein vs. TrEMBL
Match: A0A1S3JKP1 (mth938 domain-containing protein OS=Lingula unguis OX=7574 GN=LOC106174095 PE=4 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 1.350e-38
Identity = 65/120 (54.17%), Postives = 84/120 (70.00%), Query Frame = 1 
Query:  121 ISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
            ++++SWGR   +  + K  VYKD KVWPGGSR WDW ET T H PG+Q  D++E+LK+G + IV+S+GMQ+ L VP  TVDYVK     V+V PT EAV  YN + +  KRVG +FHSTC
Sbjct:   31 VTDLSWGR---MKVDGK--VYKDCKVWPGGSREWDWRETGTGHNPGVQPADLEELLKRGADTIVISRGMQSVLQVPQTTVDYVKNNHANVIVRPTEEAVKEYNRMAAAGKRVGAVFHSTC 145
[Close]
BLAST of Mth938 domain-containing protein vs. Ensembl Cavefish
Match: aamdc (adipogenesis associated Mth938 domain containing [Source:NCBI gene;Acc:107197851])

HSP 1 Score: 132.88 bits (333), Expect = 4.364e-40
Identity = 64/120 (53.33%), Postives = 81/120 (67.50%), Query Frame = 1 
Query:  121 ISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
            I+ +SWG + +   ++    YKD KVWPGGSR W+W ET T H PG+Q  D++EILKKGVE +V+ +GM   L VP  TVDYVK +GV V V  T +AV  YN L  Q  +VGG+FHSTC
Sbjct:    6 IASLSWGHMKVKGCSS---AYKDCKVWPGGSREWNWRETGTDHHPGVQPADLEEILKKGVELLVIGRGMSEALQVPPSTVDYVKKQGVDVKVLQTEKAVKEYNILAGQGAKVGGVFHSTC 122
[Close]
BLAST of Mth938 domain-containing protein vs. Ensembl Cavefish
Match: aamdc (adipogenesis associated Mth938 domain containing [Source:NCBI gene;Acc:107197851])

HSP 1 Score: 132.88 bits (333), Expect = 4.364e-40
Identity = 64/120 (53.33%), Postives = 81/120 (67.50%), Query Frame = 1 
Query:  121 ISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
            I+ +SWG + +   ++    YKD KVWPGGSR W+W ET T H PG+Q  D++EILKKGVE +V+ +GM   L VP  TVDYVK +GV V V  T +AV  YN L  Q  +VGG+FHSTC
Sbjct:    6 IASLSWGHMKVKGCSS---AYKDCKVWPGGSREWNWRETGTDHHPGVQPADLEEILKKGVELLVIGRGMSEALQVPPSTVDYVKKQGVDVKVLQTEKAVKEYNILAGQGAKVGGVFHSTC 122
[Close]
BLAST of Mth938 domain-containing protein vs. Ensembl Nematostella
Match: EDO32188 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SVS7])

HSP 1 Score: 111.309 bits (277), Expect = 4.119e-32
Identity = 56/129 (43.41%), Postives = 81/129 (62.79%), Query Frame = 1 
Query:   94 SAYVMKPGIISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
            SA      ++ ++SWG + +   +     YKD KVWPGGSR WDW+ET T  +  +Q  D+++IL   V+ +V+S+GM   L VP  TV + + +GV+V+V  T +AV  YN+L  Q  RVGG+FHSTC
Sbjct:    2 SAECHPSPLVDKLSWGHMTIGCQS-----YKDCKVWPGGSRAWDWNETGTKAR--VQPADIEDILTSKVQILVISRGMNGVLKVPPKTVQFAEARGVEVLVHLTEDAVKKYNQLAIQGARVGGVFHSTC 123
[Close]
BLAST of Mth938 domain-containing protein vs. Ensembl Medaka
Match: aamdc (adipogenesis associated Mth938 domain containing [Source:NCBI gene;Acc:101167384])

HSP 1 Score: 128.257 bits (321), Expect = 2.565e-38
Identity = 60/122 (49.18%), Postives = 83/122 (68.03%), Query Frame = 1 
Query:  121 ISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTH--HQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
            I+ +SWG + +   ++    YKD KVWPGGSR WDW ET T+  H PG+Q  D++E+LKKG E +++ +GM   L VP  T+D+VK KGV V V  T +AV  YN++ +Q  +VGG+FHSTC
Sbjct:    6 IASLSWGHMKVKGCSSS---YKDCKVWPGGSRAWDWRETGTNLQHHPGVQPADLEEVLKKGAELLIIGRGMNEALQVPPSTLDFVKQKGVDVQVFQTEKAVAEYNKMAAQGAKVGGVFHSTC 124
[Close]
BLAST of Mth938 domain-containing protein vs. Ensembl Medaka
Match: aamdc (adipogenesis associated Mth938 domain containing [Source:NCBI gene;Acc:101167384])

HSP 1 Score: 82.8037 bits (203), Expect = 6.599e-21
Identity = 46/120 (38.33%), Postives = 61/120 (50.83%), Query Frame = 1 
Query:  121 ISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
            I+ +SWG + +   ++    YKD KVWPGGSR WDW ET T+                                VP  T+D+VK KGV V V  T +AV  YN++ +Q  +VGG+FHSTC
Sbjct:    6 IASLSWGHMKVKGCSSS---YKDCKVWPGGSRAWDWRETGTN--------------------------------VPPSTLDFVKQKGVDVQVFQTEKAVAEYNKMAAQGAKVGGVFHSTC 90
[Close]
BLAST of Mth938 domain-containing protein vs. Planmine SMEST
Match: SMESG000003215.1 (SMESG000003215.1)

HSP 1 Score: 256.529 bits (654), Expect = 4.723e-89
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 1 
Query:  106 MKPGIISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 480
            MKPGIISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC
Sbjct:    1 MKPGIISEISWGRIILLDANNKPVVYKDVKVWPGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPIDTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC 125
The following BLAST results are available for this feature:
BLAST of Mth938 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
AAMDC2.895e-4049.61adipogenesis associated Mth938 domain containing [... [more]
AAMDC5.650e-4051.67adipogenesis associated Mth938 domain containing [... [more]
AAMDC5.650e-4051.67adipogenesis associated Mth938 domain containing [... [more]
AAMDC1.378e-3137.35adipogenesis associated Mth938 domain containing [... [more]
AAMDC1.927e-2140.00adipogenesis associated Mth938 domain containing [... [more]
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BLAST of Mth938 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Mth938 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Mth938 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
aamdc1.671e-3950.83adipogenesis associated, Mth938 domain containing ... [more]
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BLAST of Mth938 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSXETT00000009445.16.747e-1253.85adipogenesis associated, Mth938 domain containing ... [more]
ENSXETT00000001265.11.176e-757.14pep primary_assembly:Xenopus_tropicalis_v9.1:KV461... [more]
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BLAST of Mth938 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Aamdc2.845e-3946.56adipogenesis associated Mth938 domain containing [... [more]
Aamdc4.261e-3946.56adipogenesis associated Mth938 domain containing [... [more]
Aamdc4.261e-3946.56adipogenesis associated Mth938 domain containing [... [more]
Aamdc5.075e-3950.00adipogenesis associated Mth938 domain containing [... [more]
Aamdc1.952e-2642.20adipogenesis associated Mth938 domain containing [... [more]
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BLAST of Mth938 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q32PA8|AAMDC_BOVIN2.206e-3950.83Mth938 domain-containing protein OS=Bos taurus OX=... [more]
sp|Q9H7C9|AAMDC_HUMAN2.713e-3951.67Mth938 domain-containing protein OS=Homo sapiens O... [more]
sp|Q502H1|AAMDC_DANRE1.261e-3850.83Mth938 domain-containing protein OS=Danio rerio OX... [more]
sp|Q8R0P4|AAMDC_MOUSE3.550e-3850.00Mth938 domain-containing protein OS=Mus musculus O... [more]
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BLAST of Mth938 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
F7C2002.048e-3950.79Uncharacterized protein OS=Monodelphis domestica O... [more]
A0A5F8G8902.962e-3952.50Uncharacterized protein OS=Monodelphis domestica O... [more]
A0A4X2LA177.166e-3951.67Uncharacterized protein OS=Vombatus ursinus OX=291... [more]
A0A4X1U9241.033e-3849.61Uncharacterized protein OS=Sus scrofa OX=9823 GN=A... [more]
A0A1S3JKP11.350e-3854.17mth938 domain-containing protein OS=Lingula unguis... [more]
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BLAST of Mth938 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
aamdc4.364e-4053.33adipogenesis associated Mth938 domain containing [... [more]
aamdc4.364e-4053.33adipogenesis associated Mth938 domain containing [... [more]
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BLAST of Mth938 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Mth938 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Mth938 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO321884.119e-3243.41Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Mth938 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
aamdc2.565e-3849.18adipogenesis associated Mth938 domain containing [... [more]
aamdc6.599e-2138.33adipogenesis associated Mth938 domain containing [... [more]
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BLAST of Mth938 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000003215.14.723e-89100.00SMESG000003215.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031800 ID=SMED30031800|Name=Mth938 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=532bp
TGAAGAATAACTTTTCAAATTTAATTCATTTTATAATTATTTAAATTGAT
TATGAGATTCTATATTATCATACTTTCATTCATTTACTTTACAAGTGCTT
ATGTAATGAAACCCGGAATTATCTCCGAAATTAGTTGGGGAAGAATTATT
TTATTGGATGCAAATAATAAGCCTGTCGTGTATAAAGATGTCAAAGTTTG
GCCTGGAGGCAGCCGAAATTGGGATTGGAGTGAAACAAATACCCATCACC
AACCTGGTATACAAAAGCAAGATGTAGATGAAATTCTTAAGAAAGGTGTT
GAGTATATCGTGCTGAGTCAAGGTATGCAAAATCAATTGGGAGTTCCAAT
CGATACTGTTGATTATGTGAAATTGAAAGGCGTTAAAGTTGTAGTCGAGC
CTACTAGGGAGGCAGTGGACAGTTATAATGAGTTGGTCAGTCAGAATAAG
CGAGTCGGGGGATTGTTTCATTCTACTTGTTAACCGTTGACATTTCATTA
AGTTCGGTGTGGATTTTTAATAAAATAAATCG
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protein sequence of SMED30031800-orf-1

>SMED30031800-orf-1 ID=SMED30031800-orf-1|Name=SMED30031800-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=144bp
MRFYIIILSFIYFTSAYVMKPGIISEISWGRIILLDANNKPVVYKDVKVW
PGGSRNWDWSETNTHHQPGIQKQDVDEILKKGVEYIVLSQGMQNQLGVPI
DTVDYVKLKGVKVVVEPTREAVDSYNELVSQNKRVGGLFHSTC*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR036748MTH938-like_sf
IPR034096AAMDC
IPR007523NDUFAF3/AAMDC
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
Vocabulary: biological process
TermDefinition
GO:0043066negative regulation of apoptotic process
GO:0045600positive regulation of fat cell differentiation
GO:0045944positive regulation of transcription from RNA polymerase II promoter
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036748MTH938-like superfamilyGENE3DG3DSA:3.40.1230.10coord: 20..143
e-value: 2.1E-35
score: 123.5
IPR036748MTH938-like superfamilySUPERFAMILYSSF64076MTH938-likecoord: 24..143
IPR007523NDUFAF3/Mth938 domain-containing proteinPFAMPF04430DUF498coord: 25..140
e-value: 4.6E-15
score: 55.6
NoneNo IPR availablePANTHERPTHR15811FAMILY NOT NAMEDcoord: 24..143
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..16
score: 0.77
IPR034096Mth938 domain-containing proteinCDDcd05126Mth938coord: 24..141
e-value: 4.69923E-34
score: 113.2