Long-chain-fatty-acid--CoA ligase 4

Overview


Name	Long-chain-fatty-acid--CoA ligase 4
Smed ID	SMED30031771
Length (bp)	3026

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Long-chain-fatty-acid--CoA ligase 4 (SMED30031771) t-SNE clustered cells

Violin plots show distribution of expression levels for Long-chain-fatty-acid--CoA ligase 4 (SMED30031771) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Long-chain-fatty-acid--CoA ligase 4 (SMED30031771) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Long-chain-fatty-acid--CoA ligase 4 (SMED30031771) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30031771

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 9

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
Smed sexual biotype	SMED30031771	h1SMcG0004886	Contig42678	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30031771	h1SMcG0004886	Contig42678	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30031771	h1SMcG0004886	Contig47080	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30031771	h1SMcG0004886	Contig47080	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30031771	h1SMcG0004886	Contig39648	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30031771	h1SMcG0004886	Contig39648	newmark_ests	PMID:29674431 Fincher et al., 2018
gut	SMED30031771	h1SMcG0004886	dd_Smed_v4_3021_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30031771	h1SMcG0004886	dd_Smed_v4_3021_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30031771	h1SMcG0004886	dd_Smed_v4_3021_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Homology

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Human
Match: ACSL4 (acyl-CoA synthetase long chain family member 4 [Source:HGNC Symbol;Acc:HGNC:3571])

HSP 1 Score: 656.366 bits (1692), Expect = 0.000e+0
Identity = 341/731 (46.65%), Postives = 472/731 (64.57%), Query Frame = 3

Query:  150 ILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            IL+P+ +    L T        P+Y++  +K         N    R+ AKP  +D P  P+R++  F+ L    IPG +TLD L   A + +  K +LGTR+++  E E QPN K+FKKL    Y W  Y +++ R+ NFG GL  L +KP  + I IF ETR+EWMIAAQTCF+YN P++TLY+TLG EA+VHG+ ESEA+ +ITS EL+  KL   +     VKHII+++N        NKA+          P+G  +H M SVE +GS     + E+L     +PT   +A++MYTSGST +PKGVMM H NL+  M GQ E +P +   D YIGYLPLAHVLEL  E    ++G  +GYSSPLTL+D++SKIK  +KGD + LKPT+MA VP I+DRI K+V  K  +   +   +F+  Y+YK+ ++ +GY +   + L+F K+K LLGG VR MLSGGAPLS +   F+N+CFC PI QGYGLTE+CGAGT+    D    +VG PL   +I++++W+EG Y+  D+P PRGEI+IGG+ +++GYFK  +KT ED+ VD++G RWFCTGDIG FH DG L IIDRKKDLVKLQ GEY+SL KVE A+  CP ++  C +A+S + +VI F+ P  K+L  +A++  +E   +  IC    + + +LKE+++A     LER EIP KV +    WTP+TGLVTDA KLKRK ++  ++ +I+ MY
Sbjct:   11 ILLPVHL----LITIYSALIFIPWYFLTNAK-------KKNAMAKRIKAKP-TSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVSKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWMNYLEVNRRVNNFGSGLTALGLKPK-NTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAI-----NKAE---------YPEGFEIHSMQSVEELGS-----NPENLGIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYIQKTLFKIGYDYKLEQIKKGYDAPLCNLLLFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTINDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYSVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKRLTLLAQQKGVEGTWVD-ICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELRNHYLKDIERMY 708

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Query:  150 ILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            IL+P+ +    L T        P+Y++  +K         N    R+ AKP  +D P  P+R++  F+ L    IPG +TLD L   A + +  K +LGTR+++  E E QPN K+FKKL    Y W  Y +++ R+ NFG GL  L +KP  + I IF ETR+EWMIAAQTCF+YN P++TLY+TLG EA+VHG+ ESEA+ +ITS EL+  KL   +     VKHII+++N        NKA+          P+G  +H M SVE +GS     + E+L     +PT   +A++MYTSGST +PKGVMM H NL+  M GQ E +P +   D YIGYLPLAHVLEL  E    ++G  +GYSSPLTL+D++SKIK  +KGD + LKPT+MA VP I+DRI K+V  K  +   +   +F+  Y+YK+ ++ +GY +   + L+F K+K LLGG VR MLSGGAPLS +   F+N+CFC PI QGYGLTE+CGAGT+    D    +VG PL   +I++++W+EG Y+  D+P PRGEI+IGG+ +++GYFK  +KT ED+ VD++G RWFCTGDIG FH DG L IIDRKKDLVKLQ GEY+SL KVE A+  CP ++  C +A+S + +VI F+ P  K+L  +A++  +E   +  IC    + + +LKE+++A     LER EIP KV +    WTP+TGLVTDA KLKRK ++  ++ +I+ MY
Sbjct:   11 ILLPVHL----LITIYSALIFIPWYFLTNAK-------KKNAMAKRIKAKP-TSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVSKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWMNYLEVNRRVNNFGSGLTALGLKPK-NTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAI-----NKAE---------YPEGFEIHSMQSVEELGS-----NPENLGIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYIQKTLFKIGYDYKLEQIKKGYDAPLCNLLLFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTINDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYSVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKRLTLLAQQKGVEGTWVD-ICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELRNHYLKDIERMY 708

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Query:  150 ILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            IL+P+ +    L T        P+Y++  +K         N    R+ AKP  +D P  P+R++  F+ L    IPG +TLD L   A + +  K +LGTR+++  E E QPN K+FKKL    Y W  Y +++ R+ NFG GL  L +KP  + I IF ETR+EWMIAAQTCF+YN P++TLY+TLG EA+VHG+ ESEA+ +ITS EL+  KL   +     VKHII+++N        NKA+          P+G  +H M SVE +GS     + E+L     +PT   +A++MYTSGST +PKGVMM H NL+  M GQ E +P +   D YIGYLPLAHVLEL  E    ++G  +GYSSPLTL+D++SKIK  +KGD + LKPT+MA VP I+DRI K+V  K  +   +   +F+  Y+YK+ ++ +GY +   + L+F K+K LLGG VR MLSGGAPLS +   F+N+CFC PI QGYGLTE+CGAGT+    D    +VG PL   +I++++W+EG Y+  D+P PRGEI+IGG+ +++GYFK  +KT ED+ VD++G RWFCTGDIG FH DG L IIDRKKDLVKLQ GEY+SL KVE A+  CP ++  C +A+S + +VI F+ P  K+L  +A++  +E   +  IC    + + +LKE+++A     LER EIP KV +    WTP+TGLVTDA KLKRK ++  ++ +I+ MY
Sbjct:   11 ILLPVHL----LITIYSALIFIPWYFLTNAK-------KKNAMAKRIKAKP-TSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVSKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWMNYLEVNRRVNNFGSGLTALGLKPK-NTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAI-----NKAE---------YPEGFEIHSMQSVEELGS-----NPENLGIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYIQKTLFKIGYDYKLEQIKKGYDAPLCNLLLFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTINDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYSVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKRLTLLAQQKGVEGTWVD-ICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELRNHYLKDIERMY 708

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Query:  150 ILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            IL+P+ +    L T        P+Y++  +K         N    R+ AKP  +D P  P+R++  F+ L    IPG +TLD L   A + +  K +LGTR+++  E E QPN K+FKKL    Y W  Y +++ R+ NFG GL  L +KP  + I IF ETR+EWMIAAQTCF+YN P++TLY+TLG EA+VHG+ ESEA+ +ITS EL+  KL   +     VKHII+++N        NKA+          P+G  +H M SVE +GS     + E+L     +PT   +A++MYTSGST +PKGVMM H NL+  M GQ E +P +   D YIGYLPLAHVLEL  E    ++G  +GYSSPLTL+D++SKIK  +KGD + LKPT+MA VP I+DRI K+V  K  +   +   +F+  Y+YK+ ++ +GY +   + L+F K+K LLGG VR MLSGGAPLS +   F+N+CFC PI QGYGLTE+CGAGT+    D    +VG PL   +I++++W+EG Y+  D+P PRGEI+IGG+ +++GYFK  +KT ED+ VD++G RWFCTGDIG FH DG L IIDRKKDLVKLQ GEY+SL KVE A+  CP ++  C +A+S + +VI F+ P  K+L  +A++  +E   +  IC    + + +LKE+++A     LER EIP KV +    WTP+TGLVTDA KLKRK ++  ++ +I+ MY
Sbjct:   11 ILLPVHL----LITIYSALIFIPWYFLTNAK-------KKNAMAKRIKAKP-TSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVSKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWMNYLEVNRRVNNFGSGLTALGLKPK-NTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAI-----NKAE---------YPEGFEIHSMQSVEELGS-----NPENLGIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYIQKTLFKIGYDYKLEQIKKGYDAPLCNLLLFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTINDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYSVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKRLTLLAQQKGVEGTWVD-ICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELRNHYLKDIERMY 708

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Human
Match: ACSL4 (acyl-CoA synthetase long chain family member 4 [Source:HGNC Symbol;Acc:HGNC:3571])

HSP 1 Score: 653.67 bits (1685), Expect = 0.000e+0
Identity = 332/686 (48.40%), Postives = 456/686 (66.47%), Query Frame = 3

Query:  285 RVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            R+ AKP  +D P  P+R++  F+ L    IPG +TLD L   A + +  K +LGTR+++  E E QPN K+FKKL    Y W  Y +++ R+ NFG GL  L +KP  + I IF ETR+EWMIAAQTCF+YN P++TLY+TLG EA+VHG+ ESEA+ +ITS EL+  KL   +     VKHII+++N        NKA+          P+G  +H M SVE +GS     + E+L     +PT   +A++MYTSGST +PKGVMM H NL+  M GQ E +P +   D YIGYLPLAHVLEL  E    ++G  +GYSSPLTL+D++SKIK  +KGD + LKPT+MA VP I+DRI K+V  K  +   +   +F+  Y+YK+ ++ +GY +   + L+F K+K LLGG VR MLSGGAPLS +   F+N+CFC PI QGYGLTE+CGAGT+    D    +VG PL   +I++++W+EG Y+  D+P PRGEI+IGG+ +++GYFK  +KT ED+ VD++G RWFCTGDIG FH DG L IIDRKKDLVKLQ GEY+SL KVE A+  CP ++  C +A+S + +VI F+ P  K+L  +A++  +E   +  IC    + + +LKE+++A     LER EIP KV +    WTP+TGLVTDA KLKRK ++  ++ +I+ MY
Sbjct:    4 RIKAKPT-SDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVSKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWMNYLEVNRRVNNFGSGLTALGLKPK-NTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAI-----NKAE---------YPEGFEIHSMQSVEELGS-----NPENLGIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYIQKTLFKIGYDYKLEQIKKGYDAPLCNLLLFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTINDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYSVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKRLTLLAQQKGVEGTWVD-ICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELRNHYLKDIERMY 667

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Celegans
Match: acs-4 (Fatty Acid CoA Synthetase family [Source:UniProtKB/TrEMBL;Acc:Q20121])

HSP 1 Score: 574.318 bits (1479), Expect = 0.000e+0
Identity = 308/732 (42.08%), Postives = 443/732 (60.52%), Query Frame = 3

Query:  150 ILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICF-CPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYNR 2342
            I I + +  L++W ++Y+   +  Y +F S               RV A  +  DD   P RNI    G  +   PG +T+D +  +   L+     LGTR+L++   + QP  ++F+K    +Y W++Y +++T++     G+  L  +    +++IFAETR++W+I A  CFR N+ I+T+Y+TLG++AI H I E+EAT+++TS EL+PK+  + +KC ++K +I+     F           +  F +         +   V ++  L     E+  +S   K     +A+IMYTSG+T +PKGV++ H N++ A+ GQ +G+  I   D YIGYLPLAH+LEL  E   L+ G  VGYSSPLTL D+ASKI+  T GD  AL+PT+MA VP I+DRI K+V E+   +     E+F+  YE K +R   GY S F+D++VF KI++LLGGK+R +LSGGAPL+ E Q F+NIC  CP++QGYGLTETCGA  +    D     VG P+    I +REW EG YSP + P P+GEILI GE ++ GY+KQP+KT EDF   K G  +F TGDIG    DGSL IIDRKKDLVKLQ GEY+SLAKVE A+  CP V+  CVY    ED V+  + P  K L +IAE   +   +++ +C+ K+V +A  K+L++   +  L R EIP  +H+    WTPDTGL+T+ALKLKRK IQ  +   + D+Y +
Sbjct:   26 IWIRVAMAILRVWFFIYDCLNYLPYQLFNSP------TEKLRKSERVKANWV--DDRDGPIRNIQ---GHASETFPGKDTVDKVWRKCVELYDESPCLGTRQLIEVHEDKQPGGRVFEKWHLGEYVWQSYKEVETQVARLAAGIKDLAHEEQNPKVVIFAETRADWLITALACFRANVTIVTVYATLGEDAIAHAIGETEATILVTSSELLPKIVTLGKKCPTLKTLIY-----FAPVDQKAPAPELAPFRD---------QFKHVLSLSGLLTRNQEQVKESTAVK---SDIALIMYTSGTTGQPKGVILLHQNVVAALLGQGDGVGIICNADTYIGYLPLAHILELDAELTCLTKGAKVGYSSPLTLHDRASKIQKGTHGDCHALRPTLMAAVPAIMDRIFKAVSEEVAASPRFMQELFKLNYERKRSRYLEGYCSPFLDRIVFKKIRRLLGGKLRGVLSGGAPLNAETQRFMNICMCCPVVQGYGLTETCGAACVADINDLSTGTVGPPVRCSDIALREWSEGGYSPFNDP-PQGEILISGENISPGYYKQPEKTAEDFITYK-GKVYFATGDIGQKRDDGSLLIIDRKKDLVKLQHGEYVSLAKVECALLNCPIVDNICVYGSGMEDSVVALVVPNQKHLEKIAEAEGVSTSEMKTMCEDKKVIAAYKKQLEEHAKKSKLSRSEIPAAIHLCPEIWTPDTGLLTEALKLKRKPIQMAYQKTLDDLYKQ 727

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Celegans
Match: acs-17 (Fatty Acid CoA Synthetase family [Source:UniProtKB/TrEMBL;Acc:Q18660])

HSP 1 Score: 557.755 bits (1436), Expect = 0.000e+0
Identity = 313/735 (42.59%), Postives = 437/735 (59.46%), Query Frame = 3

Query:  156 IPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEK-CHSVKHIIFMNNYIFEGFGGNKAQDL---ITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTR---DSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICF-CPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            I + +  LK+  ++Y+  T+  + IF         +       RV A+P  N DP  PW+N+++ +GL+N+   G + L          + N+  +GTR+++K   E Q N K+F+K     Y+W +Y+ +D R+     GL  L  +     I++FAETR EWM  A  C + N PI T+Y+TLGDEA+   I E  A  + TSE L+ K+ K +     S+++IIF ++      G +   DL   + +F+ ++  G                        + Y   PTR   D LA IMYTSG+T  PKGVM++H N++   AGQ   +  +  DD+YIGYLPLAH+LE+C E VV + G+ +GYSS  TL D+A KI     GD   L+PT+MACVP ++DRI K+V ++   N  LF ++F+  YE K +R + GY S  ++KLVF +I KLLGGKVR +LSGGAPLS E Q F+NICF CP++QGYGLTETCG GT+    D     VG PL   +I ++EW E  YSP + P P+GEILI G  V LGYFK  +KTKE+ FV  +G  +F TGDIG F  DGSL IIDRKKDL+KL  GEYISL KVE  +   P V+  CVY +S++ F++  + P  K L Q+A K      + +K+C+ K+V  AL KEL +    K L+RVEIP K+ I +  WTP +GL+T+ALKLKRKNI++ F  E+ ++Y
Sbjct:   20 ITLAIVLLKIVFFIYDVVTFIPFKIFADPAEKLEKSE------RVKAEP-DNGDPTTPWKNVSAKDGLMNSSFEGIHDLGTQWEECVRRYGNQPCMGTREVLKVHKEKQSNGKIFEKWQMGDYHWRSYAQVDKRVNMIASGLTSLGQQEK-EPIILFAETREEWMTTALACMKMNFPIATMYATLGDEAVQFAINEVGAKTIFTSEALISKVKKAMSNGATSIENIIFFDSVDPASRGESVDVDLPFTLLSFDQLLSRG------------------------EPYPV-PTRSKKDDLAFIMYTSGTTGNPKGVMISHQNIVAVTAGQAFNIV-LGPDDRYIGYLPLAHILEVCAEMVVFTKGVRIGYSSAQTLFDRAPKIMKGEHGDCWVLQPTLMACVPAVMDRIFKAVIDEVNANSELFKQLFKTCYERKRSRYEEGYTSFILNKLVFNRIGKLLGGKVRQILSGGAPLSPETQRFMNICFCCPVVQGYGLTETCGGGTIADIHDLSTGTVGPPLTCCEILLQEWAEAGYSPKNDP-PQGEILISGPNVALGYFKNDEKTKEE-FVKVNGKTFFATGDIGEFREDGSLKIIDRKKDLLKLAHGEYISLGKVETNLLTNPNVDNVCVYGDSNKSFLVALVVPNQKNLTQLASKAGASNDNFEKLCEDKKVVEALQKELAQYGASK-LQRVEIPQKIFICHEPWTPASGLLTEALKLKRKNIEKAFRKELDELY 717

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Query:  156 IPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEK-CHSVKHIIFMNNYIFEGFGGNKAQDL---ITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTR---DSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICF-CPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            I + +  LK+  ++Y+  T+  + IF         +       RV A+P  N DP  PW+N+++ +GL+N+   G + L          + N+  +GTR+++K   E Q N K+F+K     Y+W +Y+ +D R+     GL  L  +     I++FAETR EWM  A  C + N PI T+Y+TLGDEA+   I E  A  + TSE L+ K+ K +     S+++IIF ++      G +   DL   + +F+ ++  G                        + Y   PTR   D LA IMYTSG+T  PKGVM++H N++   AGQ   +  +  DD+YIGYLPLAH+LE+C E VV + G+ +GYSS  TL D+A KI     GD   L+PT+MACVP ++DRI K+V ++   N  LF ++F+  YE K +R + GY S  ++KLVF +I KLLGGKVR +LSGGAPLS E Q F+NICF CP++QGYGLTETCG GT+    D     VG PL   +I ++EW E  YSP + P P+GEILI G  V LGYFK  +KTKE+ FV  +G  +F TGDIG F  DGSL IIDRKKDL+KL  GEYISL KVE  +   P V+  CVY +S++ F++  + P  K L Q+A K      + +K+C+ K+V  AL KEL +    K L+RVEIP K+ I +  WTP +GL+T+ALKLKRKNI++ F  E+ ++Y
Sbjct:   20 ITLAIVLLKIVFFIYDVVTFIPFKIFADPAEKLEKSE------RVKAEP-DNGDPTTPWKNVSAKDGLMNSSFEGIHDLGTQWEECVRRYGNQPCMGTREVLKVHKEKQSNGKIFEKWQMGDYHWRSYAQVDKRVNMIASGLTSLGQQEK-EPIILFAETREEWMTTALACMKMNFPIATMYATLGDEAVQFAINEVGAKTIFTSEALISKVKKAMSNGATSIENIIFFDSVDPASRGESVDVDLPFTLLSFDQLLSRG------------------------EPYPV-PTRSKKDDLAFIMYTSGTTGNPKGVMISHQNIVAVTAGQAFNIV-LGPDDRYIGYLPLAHILEVCAEMVVFTKGVRIGYSSAQTLFDRAPKIMKGEHGDCWVLQPTLMACVPAVMDRIFKAVIDEVNANSELFKQLFKTCYERKRSRYEEGYTSFILNKLVFNRIGKLLGGKVRQILSGGAPLSPETQRFMNICFCCPVVQGYGLTETCGGGTIADIHDLSTGTVGPPLTCCEILLQEWAEAGYSPKNDP-PQGEILISGPNVALGYFKNDEKTKEE-FVKVNGKTFFATGDIGEFREDGSLKIIDRKKDLLKLAHGEYISLGKVETNLLTNPNVDNVCVYGDSNKSFLVALVVPNQKNLTQLASKAGASNDNFEKLCEDKKVVEALQKELAQYGASK-LQRVEIPQKIFICHEPWTPASGLLTEALKLKRKNIEKAFRKELDELY 717

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Query:  156 IPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEK-CHSVKHIIFMNNYIFEGFGGNKAQDL---ITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTR---DSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICF-CPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            I + +  LK+  ++Y+  T+  + IF         +       RV A+P  N DP  PW+N+++ +GL+N+   G + L          + N+  +GTR+++K   E Q N K+F+K     Y+W +Y+ +D R+     GL  L  +     I++FAETR EWM  A  C + N PI T+Y+TLGDEA+   I E  A  + TSE L+ K+ K +     S+++IIF ++      G +   DL   + +F+ ++  G                        + Y   PTR   D LA IMYTSG+T  PKGVM++H N++   AGQ   +  +  DD+YIGYLPLAH+LE+C E VV + G+ +GYSS  TL D+A KI     GD   L+PT+MACVP ++DRI K+V ++   N  LF ++F+  YE K +R + GY S  ++KLVF +I KLLGGKVR +LSGGAPLS E Q F+NICF CP++QGYGLTETCG GT+    D     VG PL   +I ++EW E  YSP + P P+GEILI G  V LGYFK  +KTKE+ FV  +G  +F TGDIG F  DGSL IIDRKKDL+KL  GEYISL KVE  +   P V+  CVY +S++ F++  + P  K L Q+A K      + +K+C+ K+V  AL KEL +    K L+RVEIP K+ I +  WTP +GL+T+ALKLKRKNI++ F  E+ ++Y
Sbjct:   20 ITLAIVLLKIVFFIYDVVTFIPFKIFADPAEKLEKSE------RVKAEP-DNGDPTTPWKNVSAKDGLMNSSFEGIHDLGTQWEECVRRYGNQPCMGTREVLKVHKEKQSNGKIFEKWQMGDYHWRSYAQVDKRVNMIASGLTSLGQQEK-EPIILFAETREEWMTTALACMKMNFPIATMYATLGDEAVQFAINEVGAKTIFTSEALISKVKKAMSNGATSIENIIFFDSVDPASRGESVDVDLPFTLLSFDQLLSRG------------------------EPYPV-PTRSKKDDLAFIMYTSGTTGNPKGVMISHQNIVAVTAGQAFNIV-LGPDDRYIGYLPLAHILEVCAEMVVFTKGVRIGYSSAQTLFDRAPKIMKGEHGDCWVLQPTLMACVPAVMDRIFKAVIDEVNANSELFKQLFKTCYERKRSRYEEGYTSFILNKLVFNRIGKLLGGKVRQILSGGAPLSPETQRFMNICFCCPVVQGYGLTETCGGGTIADIHDLSTGTVGPPLTCCEILLQEWAEAGYSPKNDP-PQGEILISGPNVALGYFKNDEKTKEE-FVKVNGKTFFATGDIGEFREDGSLKIIDRKKDLLKLAHGEYISLGKVETNLLTNPNVDNVCVYGDSNKSFLVALVVPNQKNLTQLASKAGASNDNFEKLCEDKKVVEALQKELAQYGASK-LQRVEIPQKIFICHEPWTPASGLLTEALKLKRKNIEKAFRKELDELY 717

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Celegans
Match: acs-3 (Fatty Acid CoA Synthetase family [Source:UniProtKB/TrEMBL;Acc:O44560])

HSP 1 Score: 251.136 bits (640), Expect = 1.281e-71
Identity = 184/635 (28.98%), Postives = 315/635 (49.61%), Query Frame = 3

Query:  483 PNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVV-ITSEELMPKLCKIIEKCHSVKHIIFMNNY-IFEGFGGNKAQDLITTFENVV--PDGITLHEMGSVENMG--SLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHR-----DDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYP--SVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICF-CPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFIS---PKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            PN  LF +    ++ + TY +     T  G G++ +  K   +  LI         IA     +Y                +HGI   + T V +  +  +  LC II+ C     IIF  N    EGF  +K  D + + + ++      TL +   VE M     K+ G     +    KP  ++  +I +TSG+T +PKGV M+HG+L+T+++G F      ++     +D Y  +L LAH+ E   + + +  G  +G     T         A     I  L+PTI++ VP +L+++ ++V +     G +  ++F +A + K  ++ RG      F DK+VF K+K +LGGK++ + SGGAP+++E + F    + CP+++GYG TE   AGTL +  D     VG P    Q+++ +  E NY  ++     GE+   G  +  GYF+ P+ T +   +D +G  W  TGDIG +   G L IIDRK ++ KL  G+++S  ++E   +  P +    V  ++   F++  +    P+ KQL  + + ++ +E  I+ I + KEV +A++ EL K   + GL+ +E+   VH+   ++T + GL+T  LK +RK +++ F  +I  MY
Sbjct:   56 PNAPLFGESVNGRHVYTTYREAVEEATIIGSGIVAIMQKNQSTNKLI--------GIAGIHSRKYM-------------HTMHGISGFDLTTVPLYHQSKLETLCDIIDNCKL--EIIFCENAKRAEGFLSSKLGDRLRSLKTLIILDKTTTLQKHDDVEVMSLDEFKEIGKRNKRNPV--KPKPETTYVICHTSGTTGRPKGVEMSHGSLVTSVSGIFTSWTIAYKWKFGLEDTYFSFLSLAHIYEHLIQTLTIYFGGKIGIYDGNT---------ATLIPQIQKLEPTIVSLVPRLLNKLYEAVHDGVAKKGFIARKLFDYAKKTKARQLQRGVLRYDTFFDKIVFKKLKLMLGGKLKVLTSGGAPITKEVKTFTRYAYGCPLVEGYGQTECSAAGTLTLPWDTTYGNVGGPAPWAQVKLVDVPEKNYFSMND---EGEVCFRGNAMMTGYFQDPELTAKT--IDSEG--WLHTGDIGKWLPTGGLQIIDRKNEMFKLSQGDFVSPIQIEAIYANSPLITQIYVTGKTERSFLVGIVVLDLPRFKQL-PLVKALDGKE-SIEMIMENKEVKNAVIAELNKYAKQNGLQTIELIRNVHLTLQEFTEENGLITSTLKNRRKILEDYFAPQIAKMY 647

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Fly
Match: Acsl (gene:FBgn0263120 transcript:FBtr0088700)

HSP 1 Score: 553.518 bits (1425), Expect = 0.000e+0
Identity = 301/732 (41.12%), Postives = 430/732 (58.74%), Query Frame = 3

Query:  147 SILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGL-VNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            S  +   + A+K   +VY+  T P Y + Q                RV AK ++NDD +  +R  +    + V  +    +TL+ +    A  + +K  LGTR+++  E E Q N ++FKK     Y W+T+++ +    NFGRGL +L  KP    I+IFAETR+EWMIAA  CF+  +PI+T+Y+TLGD+ + H I E+E T VITS +L+PK   +++KC  VK II++ +             L  T      +G+ +     V   G    +            P  D +A+IMYTSGST  PKGV+++H N +  M G F  + PI+ DD  IG+LPLAHV EL  E+V L  G+P+GYS+PLTL D +SKIK   KGD + LKPT M  VP ILDRI+K + +K          +F++ Y+YK+  + RGY +  +DKLVF K+ KL+GGKVR ++SGGAPLS +    I  C C  +IQGYGLTET    T+M   D    + G PL    I++  W+EGNY   ++P P+GE+LIGGECV+ GY+K P KT EDFF ++DG RWF TGDIG   +DG L IIDRKKDLVKLQ GEY+SL KVE  +  C  +E  CVY + ++ + +  + P    L ++A+K  + +K  +++C    +  A+LKE+ +   +  L++ E+P  + +    W+PD GLVT A KLKRK+IQ+R+  +I  MY
Sbjct:   11 SFWVQSAIGAIKAIAFVYDIITLPVYLVLQKPW------KRRQDSRRVKAKIVRNDDNELTYRTTDPPRDVHVKMLQENIDTLEKVFNYVAKTYTSKRCLGTRQILSEEDEVQQNGRVFKKYNLGDYKWKTFTEAERTAANFGRGLRELGQKPR-ENIVIFAETRAEWMIAAHGCFKQAMPIVTVYATLGDDGVAHCITETEVTTVITSHDLLPKFKTLLDKCPLVKTIIYIED------------QLQKTETTGFKEGVKILPFNQVVKTGQDSKFEH--------VPPKGDDIAIIMYTSGSTGTPKGVLLSHKNCIATMKG-FVDMVPIYPDDVLIGFLPLAHVFELVAESVCLMTGVPIGYSTPLTLIDTSSKIKRGCKGDATVLKPTCMTSVPLILDRISKGINDKVNSGSAFKKSLFKFLYQYKVKWVQRGYKTPLIDKLVFKKVAKLMGGKVRIIMSGGAPLSADTHEQIKTCLCLELIQGYGLTETTSGATVMDYRDMTYGRTGGPLTVCDIRLVNWEEGNYRVTNKPYPQGEVLIGGECVSQGYYKLPGKTNEDFF-EEDGQRWFKTGDIGEIQADGVLKIIDRKKDLVKLQAGEYVSLGKVESELKTCGIIENICVYGDPTKQYTVALVVPNQNHLEELAQKHGLGDKSFEELCSSPIIEKAILKEIAEHARKCKLQKYEVPAAITLCKEVWSPDMGLVTAAFKLKRKDIQDRYQHDINRMY 713

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Fly
Match: Acsl (gene:FBgn0263120 transcript:FBtr0100426)

HSP 1 Score: 553.518 bits (1425), Expect = 0.000e+0
Identity = 301/732 (41.12%), Postives = 430/732 (58.74%), Query Frame = 3

Query:  147 SILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGL-VNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            S  +   + A+K   +VY+  T P Y + Q                RV AK ++NDD +  +R  +    + V  +    +TL+ +    A  + +K  LGTR+++  E E Q N ++FKK     Y W+T+++ +    NFGRGL +L  KP    I+IFAETR+EWMIAA  CF+  +PI+T+Y+TLGD+ + H I E+E T VITS +L+PK   +++KC  VK II++ +             L  T      +G+ +     V   G    +            P  D +A+IMYTSGST  PKGV+++H N +  M G F  + PI+ DD  IG+LPLAHV EL  E+V L  G+P+GYS+PLTL D +SKIK   KGD + LKPT M  VP ILDRI+K + +K          +F++ Y+YK+  + RGY +  +DKLVF K+ KL+GGKVR ++SGGAPLS +    I  C C  +IQGYGLTET    T+M   D    + G PL    I++  W+EGNY   ++P P+GE+LIGGECV+ GY+K P KT EDFF ++DG RWF TGDIG   +DG L IIDRKKDLVKLQ GEY+SL KVE  +  C  +E  CVY + ++ + +  + P    L ++A+K  + +K  +++C    +  A+LKE+ +   +  L++ E+P  + +    W+PD GLVT A KLKRK+IQ+R+  +I  MY
Sbjct:   13 SFWVQSAIGAIKAIAFVYDIITLPVYLVLQKPW------KRRQDSRRVKAKIVRNDDNELTYRTTDPPRDVHVKMLQENIDTLEKVFNYVAKTYTSKRCLGTRQILSEEDEVQQNGRVFKKYNLGDYKWKTFTEAERTAANFGRGLRELGQKPR-ENIVIFAETRAEWMIAAHGCFKQAMPIVTVYATLGDDGVAHCITETEVTTVITSHDLLPKFKTLLDKCPLVKTIIYIED------------QLQKTETTGFKEGVKILPFNQVVKTGQDSKFEH--------VPPKGDDIAIIMYTSGSTGTPKGVLLSHKNCIATMKG-FVDMVPIYPDDVLIGFLPLAHVFELVAESVCLMTGVPIGYSTPLTLIDTSSKIKRGCKGDATVLKPTCMTSVPLILDRISKGINDKVNSGSAFKKSLFKFLYQYKVKWVQRGYKTPLIDKLVFKKVAKLMGGKVRIIMSGGAPLSADTHEQIKTCLCLELIQGYGLTETTSGATVMDYRDMTYGRTGGPLTVCDIRLVNWEEGNYRVTNKPYPQGEVLIGGECVSQGYYKLPGKTNEDFF-EEDGQRWFKTGDIGEIQADGVLKIIDRKKDLVKLQAGEYVSLGKVESELKTCGIIENICVYGDPTKQYTVALVVPNQNHLEELAQKHGLGDKSFEELCSSPIIEKAILKEIAEHARKCKLQKYEVPAAITLCKEVWSPDMGLVTAAFKLKRKDIQDRYQHDINRMY 715

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Fly
Match: Acsl (gene:FBgn0263120 transcript:FBtr0088699)

HSP 1 Score: 553.518 bits (1425), Expect = 0.000e+0
Identity = 301/732 (41.12%), Postives = 430/732 (58.74%), Query Frame = 3

Query:  147 SILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGL-VNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            S  +   + A+K   +VY+  T P Y + Q                RV AK ++NDD +  +R  +    + V  +    +TL+ +    A  + +K  LGTR+++  E E Q N ++FKK     Y W+T+++ +    NFGRGL +L  KP    I+IFAETR+EWMIAA  CF+  +PI+T+Y+TLGD+ + H I E+E T VITS +L+PK   +++KC  VK II++ +             L  T      +G+ +     V   G    +            P  D +A+IMYTSGST  PKGV+++H N +  M G F  + PI+ DD  IG+LPLAHV EL  E+V L  G+P+GYS+PLTL D +SKIK   KGD + LKPT M  VP ILDRI+K + +K          +F++ Y+YK+  + RGY +  +DKLVF K+ KL+GGKVR ++SGGAPLS +    I  C C  +IQGYGLTET    T+M   D    + G PL    I++  W+EGNY   ++P P+GE+LIGGECV+ GY+K P KT EDFF ++DG RWF TGDIG   +DG L IIDRKKDLVKLQ GEY+SL KVE  +  C  +E  CVY + ++ + +  + P    L ++A+K  + +K  +++C    +  A+LKE+ +   +  L++ E+P  + +    W+PD GLVT A KLKRK+IQ+R+  +I  MY
Sbjct:   11 SFWVQSAIGAIKAIAFVYDIITLPVYLVLQKPW------KRRQDSRRVKAKIVRNDDNELTYRTTDPPRDVHVKMLQENIDTLEKVFNYVAKTYTSKRCLGTRQILSEEDEVQQNGRVFKKYNLGDYKWKTFTEAERTAANFGRGLRELGQKPR-ENIVIFAETRAEWMIAAHGCFKQAMPIVTVYATLGDDGVAHCITETEVTTVITSHDLLPKFKTLLDKCPLVKTIIYIED------------QLQKTETTGFKEGVKILPFNQVVKTGQDSKFEH--------VPPKGDDIAIIMYTSGSTGTPKGVLLSHKNCIATMKG-FVDMVPIYPDDVLIGFLPLAHVFELVAESVCLMTGVPIGYSTPLTLIDTSSKIKRGCKGDATVLKPTCMTSVPLILDRISKGINDKVNSGSAFKKSLFKFLYQYKVKWVQRGYKTPLIDKLVFKKVAKLMGGKVRIIMSGGAPLSADTHEQIKTCLCLELIQGYGLTETTSGATVMDYRDMTYGRTGGPLTVCDIRLVNWEEGNYRVTNKPYPQGEVLIGGECVSQGYYKLPGKTNEDFF-EEDGQRWFKTGDIGEIQADGVLKIIDRKKDLVKLQAGEYVSLGKVESELKTCGIIENICVYGDPTKQYTVALVVPNQNHLEELAQKHGLGDKSFEELCSSPIIEKAILKEIAEHARKCKLQKYEVPAAITLCKEVWSPDMGLVTAAFKLKRKDIQDRYQHDINRMY 713

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Fly
Match: Acsl (gene:FBgn0263120 transcript:FBtr0100427)

HSP 1 Score: 553.518 bits (1425), Expect = 0.000e+0
Identity = 301/732 (41.12%), Postives = 430/732 (58.74%), Query Frame = 3

Query:  147 SILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGL-VNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            S  +   + A+K   +VY+  T P Y + Q                RV AK ++NDD +  +R  +    + V  +    +TL+ +    A  + +K  LGTR+++  E E Q N ++FKK     Y W+T+++ +    NFGRGL +L  KP    I+IFAETR+EWMIAA  CF+  +PI+T+Y+TLGD+ + H I E+E T VITS +L+PK   +++KC  VK II++ +             L  T      +G+ +     V   G    +            P  D +A+IMYTSGST  PKGV+++H N +  M G F  + PI+ DD  IG+LPLAHV EL  E+V L  G+P+GYS+PLTL D +SKIK   KGD + LKPT M  VP ILDRI+K + +K          +F++ Y+YK+  + RGY +  +DKLVF K+ KL+GGKVR ++SGGAPLS +    I  C C  +IQGYGLTET    T+M   D    + G PL    I++  W+EGNY   ++P P+GE+LIGGECV+ GY+K P KT EDFF ++DG RWF TGDIG   +DG L IIDRKKDLVKLQ GEY+SL KVE  +  C  +E  CVY + ++ + +  + P    L ++A+K  + +K  +++C    +  A+LKE+ +   +  L++ E+P  + +    W+PD GLVT A KLKRK+IQ+R+  +I  MY
Sbjct:   11 SFWVQSAIGAIKAIAFVYDIITLPVYLVLQKPW------KRRQDSRRVKAKIVRNDDNELTYRTTDPPRDVHVKMLQENIDTLEKVFNYVAKTYTSKRCLGTRQILSEEDEVQQNGRVFKKYNLGDYKWKTFTEAERTAANFGRGLRELGQKPR-ENIVIFAETRAEWMIAAHGCFKQAMPIVTVYATLGDDGVAHCITETEVTTVITSHDLLPKFKTLLDKCPLVKTIIYIED------------QLQKTETTGFKEGVKILPFNQVVKTGQDSKFEH--------VPPKGDDIAIIMYTSGSTGTPKGVLLSHKNCIATMKG-FVDMVPIYPDDVLIGFLPLAHVFELVAESVCLMTGVPIGYSTPLTLIDTSSKIKRGCKGDATVLKPTCMTSVPLILDRISKGINDKVNSGSAFKKSLFKFLYQYKVKWVQRGYKTPLIDKLVFKKVAKLMGGKVRIIMSGGAPLSADTHEQIKTCLCLELIQGYGLTETTSGATVMDYRDMTYGRTGGPLTVCDIRLVNWEEGNYRVTNKPYPQGEVLIGGECVSQGYYKLPGKTNEDFF-EEDGQRWFKTGDIGEIQADGVLKIIDRKKDLVKLQAGEYVSLGKVESELKTCGIIENICVYGDPTKQYTVALVVPNQNHLEELAQKHGLGDKSFEELCSSPIIEKAILKEIAEHARKCKLQKYEVPAAITLCKEVWSPDMGLVTAAFKLKRKDIQDRYQHDINRMY 713

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Fly
Match: Acsl (gene:FBgn0263120 transcript:FBtr0100430)

HSP 1 Score: 553.518 bits (1425), Expect = 0.000e+0
Identity = 301/732 (41.12%), Postives = 430/732 (58.74%), Query Frame = 3

Query:  147 SILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGL-VNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            S  +   + A+K   +VY+  T P Y + Q                RV AK ++NDD +  +R  +    + V  +    +TL+ +    A  + +K  LGTR+++  E E Q N ++FKK     Y W+T+++ +    NFGRGL +L  KP    I+IFAETR+EWMIAA  CF+  +PI+T+Y+TLGD+ + H I E+E T VITS +L+PK   +++KC  VK II++ +             L  T      +G+ +     V   G    +            P  D +A+IMYTSGST  PKGV+++H N +  M G F  + PI+ DD  IG+LPLAHV EL  E+V L  G+P+GYS+PLTL D +SKIK   KGD + LKPT M  VP ILDRI+K + +K          +F++ Y+YK+  + RGY +  +DKLVF K+ KL+GGKVR ++SGGAPLS +    I  C C  +IQGYGLTET    T+M   D    + G PL    I++  W+EGNY   ++P P+GE+LIGGECV+ GY+K P KT EDFF ++DG RWF TGDIG   +DG L IIDRKKDLVKLQ GEY+SL KVE  +  C  +E  CVY + ++ + +  + P    L ++A+K  + +K  +++C    +  A+LKE+ +   +  L++ E+P  + +    W+PD GLVT A KLKRK+IQ+R+  +I  MY
Sbjct:   11 SFWVQSAIGAIKAIAFVYDIITLPVYLVLQKPW------KRRQDSRRVKAKIVRNDDNELTYRTTDPPRDVHVKMLQENIDTLEKVFNYVAKTYTSKRCLGTRQILSEEDEVQQNGRVFKKYNLGDYKWKTFTEAERTAANFGRGLRELGQKPR-ENIVIFAETRAEWMIAAHGCFKQAMPIVTVYATLGDDGVAHCITETEVTTVITSHDLLPKFKTLLDKCPLVKTIIYIED------------QLQKTETTGFKEGVKILPFNQVVKTGQDSKFEH--------VPPKGDDIAIIMYTSGSTGTPKGVLLSHKNCIATMKG-FVDMVPIYPDDVLIGFLPLAHVFELVAESVCLMTGVPIGYSTPLTLIDTSSKIKRGCKGDATVLKPTCMTSVPLILDRISKGINDKVNSGSAFKKSLFKFLYQYKVKWVQRGYKTPLIDKLVFKKVAKLMGGKVRIIMSGGAPLSADTHEQIKTCLCLELIQGYGLTETTSGATVMDYRDMTYGRTGGPLTVCDIRLVNWEEGNYRVTNKPYPQGEVLIGGECVSQGYYKLPGKTNEDFF-EEDGQRWFKTGDIGEIQADGVLKIIDRKKDLVKLQAGEYVSLGKVESELKTCGIIENICVYGDPTKQYTVALVVPNQNHLEELAQKHGLGDKSFEELCSSPIIEKAILKEIAEHARKCKLQKYEVPAAITLCKEVWSPDMGLVTAAFKLKRKDIQDRYQHDINRMY 713

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Zebrafish
Match: acsl3b (acyl-CoA synthetase long chain family member 3b [Source:ZFIN;Acc:ZDB-GENE-030131-492])

HSP 1 Score: 625.935 bits (1613), Expect = 0.000e+0
Identity = 332/729 (45.54%), Postives = 446/729 (61.18%), Query Frame = 3

Query:  159 PITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            P+ +   +   +VY   T+  +Y+      LS  + N     RV A+ I N +P  P+R +NS   L + +  G +TLD +   A   +P +  LGTR+L+  E E QPN K+FKK+    Y W +Y D       FG GL  L  KP +  I IF ETR+EW+IAAQ CF YN P++TLYSTLG  AI HG+ E+E T ++TS++L+  +L  I+ +   +KHII +              D   T    +P GI +H M +V+ +GS       E++     +P    +A+IMYTSGST  PKGVM++H N++  + G  E +P +  +D YIGYLPLAHVLEL  E V +SHG  +GYSSP TL D++SKIK  +KGD S LKPT+MA VP I+DRI K+V  K  +   +   +F  AY YKM ++ +GY +   D+LVF K++ LLGG  R +LSGGAPLS   Q F+NICFC P+ QGYGLTETCGAGT+    D    +VG PL   +I +++W+EG Y   D+P PRGEILIGG  VT+GY+K   K +EDFF DK+G RWFCTGDIG FH DG L IIDRKKDLVKLQ GEY+SL KVE  +  CP ++  C YA S E +VI F+ P  KQL  +A +  I     ++IC   E+   +L+ +  A +   LER EIP K+ +    WTP+TGLVTDA KLKRK ++  +  +I+ MY 
Sbjct:    9 PLLLQVFRSVVWVYSVITFLPWYL------LSGASGNQARAKRVKARAI-NGNPAGPYRAVNSQLKLASLLHEGVDTLDKVFEYAVVHFPERDCLGTRELLSEEDEIQPNGKVFKKVILGDYNWLSYQDTFHLAQRFGSGLAALGQKP-LCNIAIFCETRAEWVIAAQACFMYNFPLVTLYSTLGGSAIAHGLNETEVTHIVTSKDLLQSRLKAILLEVPRLKHIIVV--------------DTKPTSWPDLPRGIMVHNMAAVQELGS-----KPENIMVSRRQPVPSDIAVIMYTSGSTGIPKGVMISHSNIIAGITGMAERIPNLDENDTYIGYLPLAHVLELSAELVCVSHGCRIGYSSPQTLADQSSKIKKGSKGDTSVLKPTLMAAVPEIMDRIYKNVMTKVEEMSSVQKTLFVLAYNYKMEQISKGYTTPLCDRLVFRKVRSLLGGNTRVLLSGGAPLSAATQRFMNICFCCPVGQGYGLTETCGAGTISEMSDYSTGRVGAPLVCSEIMLKDWEEGGYFSTDKPNPRGEILIGGPNVTMGYYKNEQKNREDFFEDKNGQRWFCTGDIGEFHPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAVLKNCPLIDNICAYANSDESYVIGFVVPNQKQLTALAGQKGI-RGSWEEICNHAEMEKEVLRIITDAAISGKLERFEIPKKICLSAEPWTPETGLVTDAFKLKRKELKTHYQDDIERMYG 709

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Query:  159 PITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            P+ +   +   +VY   T+  +Y+      LS  + N     RV A+ I N +P  P+R +NS   L + +  G +TLD +   A   +P +  LGTR+L+  E E QPN K+FKK+    Y W +Y D       FG GL  L  KP +  I IF ETR+EW+IAAQ CF YN P++TLYSTLG  AI HG+ E+E T ++TS++L+  +L  I+ +   +KHII +              D   T    +P GI +H M +V+ +GS       E++     +P    +A+IMYTSGST  PKGVM++H N++  + G  E +P +  +D YIGYLPLAHVLEL  E V +SHG  +GYSSP TL D++SKIK  +KGD S LKPT+MA VP I+DRI K+V  K  +   +   +F  AY YKM ++ +GY +   D+LVF K++ LLGG  R +LSGGAPLS   Q F+NICFC P+ QGYGLTETCGAGT+    D    +VG PL   +I +++W+EG Y   D+P PRGEILIGG  VT+GY+K   K +EDFF DK+G RWFCTGDIG FH DG L IIDRKKDLVKLQ GEY+SL KVE  +  CP ++  C YA S E +VI F+ P  KQL  +A +  I     ++IC   E+   +L+ +  A +   LER EIP K+ +    WTP+TGLVTDA KLKRK ++  +  +I+ MY 
Sbjct:    9 PLLLQVFRSVVWVYSVITFLPWYL------LSGASGNQARAKRVKARAI-NGNPAGPYRAVNSQLKLASLLHEGVDTLDKVFEYAVVHFPERDCLGTRELLSEEDEIQPNGKVFKKVILGDYNWLSYQDTFHLAQRFGSGLAALGQKP-LCNIAIFCETRAEWVIAAQACFMYNFPLVTLYSTLGGSAIAHGLNETEVTHIVTSKDLLQSRLKAILLEVPRLKHIIVV--------------DTKPTSWPDLPRGIMVHNMAAVQELGS-----KPENIMVSRRQPVPSDIAVIMYTSGSTGIPKGVMISHSNIIAGITGMAERIPNLDENDTYIGYLPLAHVLELSAELVCVSHGCRIGYSSPQTLADQSSKIKKGSKGDTSVLKPTLMAAVPEIMDRIYKNVMTKVEEMSSVQKTLFVLAYNYKMEQISKGYTTPLCDRLVFRKVRSLLGGNTRVLLSGGAPLSAATQRFMNICFCCPVGQGYGLTETCGAGTISEMSDYSTGRVGAPLVCSEIMLKDWEEGGYFSTDKPNPRGEILIGGPNVTMGYYKNEQKNREDFFEDKNGQRWFCTGDIGEFHPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAVLKNCPLIDNICAYANSDESYVIGFVVPNQKQLTALAGQKGI-RGSWEEICNHAEMEKEVLRIITDAAISGKLERFEIPKKICLSAEPWTPETGLVTDAFKLKRKELKTHYQDDIERMYG 709

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Query:  159 PITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            P+ +   +   +VY   T+  +Y+      LS  + N     RV A+ I N +P  P+R +NS   L + +  G +TLD +   A   +P +  LGTR+L+  E E QPN K+FKK+    Y W +Y D       FG GL  L  KP +  I IF ETR+EW+IAAQ CF YN P++TLYSTLG  AI HG+ E+E T ++TS++L+  +L  I+ +   +KHII +              D   T    +P GI +H M +V+ +GS       E++     +P    +A+IMYTSGST  PKGVM++H N++  + G  E +P +  +D YIGYLPLAHVLEL  E V +SHG  +GYSSP TL D++SKIK  +KGD S LKPT+MA VP I+DRI K+V  K  +   +   +F  AY YKM ++ +GY +   D+LVF K++ LLGG  R +LSGGAPLS   Q F+NICFC P+ QGYGLTETCGAGT+    D    +VG PL   +I +++W+EG Y   D+P PRGEILIGG  VT+GY+K   K +EDFF DK+G RWFCTGDIG FH DG L IIDRKKDLVKLQ GEY+SL KVE  +  CP ++  C YA S E +VI F+ P  KQL  +A +  I     ++IC   E+   +L+ +  A +   LER EIP K+ +    WTP+TGLVTDA KLKRK ++  +  +I+ MY 
Sbjct:    9 PLLLQVFRSVVWVYSVITFLPWYL------LSGASGNQARAKRVKARAI-NGNPAGPYRAVNSQLKLASLLHEGVDTLDKVFEYAVVHFPERDCLGTRELLSEEDEIQPNGKVFKKVILGDYNWLSYQDTFHLAQRFGSGLAALGQKP-LCNIAIFCETRAEWVIAAQACFMYNFPLVTLYSTLGGSAIAHGLNETEVTHIVTSKDLLQSRLKAILLEVPRLKHIIVV--------------DTKPTSWPDLPRGIMVHNMAAVQELGS-----KPENIMVSRRQPVPSDIAVIMYTSGSTGIPKGVMISHSNIIAGITGMAERIPNLDENDTYIGYLPLAHVLELSAELVCVSHGCRIGYSSPQTLADQSSKIKKGSKGDTSVLKPTLMAAVPEIMDRIYKNVMTKVEEMSSVQKTLFVLAYNYKMEQISKGYTTPLCDRLVFRKVRSLLGGNTRVLLSGGAPLSAATQRFMNICFCCPVGQGYGLTETCGAGTISEMSDYSTGRVGAPLVCSEIMLKDWEEGGYFSTDKPNPRGEILIGGPNVTMGYYKNEQKNREDFFEDKNGQRWFCTGDIGEFHPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAVLKNCPLIDNICAYANSDESYVIGFVVPNQKQLTALAGQKGI-RGSWEEICNHAEMEKEVLRIITDAAISGKLERFEIPKKICLSAEPWTPETGLVTDAFKLKRKELKTHYQDDIERMYG 709

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Zebrafish
Match: acsl4a (acyl-CoA synthetase long chain family member 4a [Source:ZFIN;Acc:ZDB-GENE-040426-1565])

HSP 1 Score: 619.002 bits (1595), Expect = 0.000e+0
Identity = 323/736 (43.89%), Postives = 459/736 (62.36%), Query Frame = 3

Query:  144 MSILIPITVNALKLWTY-VYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYNR 2342
            +S ++   V+A+ +W Y +  F  W F    Q K  L+          + L     +D P  P+R+ + F  LV    PG +TLD L   A   +     LGTR+++  + E QPN K+FKKL   +Y W++Y ++D  +++FG GL  L  +P  S I IF ETR+EWMI AQ CFR N P++T Y+TLG++A+ +G+ ES  T ++TS EL+  KL  ++ +  ++KHII+++N      G               P GI +H M SVE +G+       E+L+  + KP    LA+IMYTSGST +PKGVM+ H NL+  MAGQ E +P +   D YI YLPLAHVLE+  E   +++G  +GYSSP TL+D+++KIK  +KGD S LKP++MA VP I+DRI K+V  K  +   +   +F+ AY+YK+ ++ +GY +   + ++F K++ LLGG VR MLSGGAPLS   Q F+NICFC P+ QGYGLTETCGAGT+    D    +VG PL   ++++++W EG Y+  D+P PRGEILIGG  VT+GY++      +DFFVD++G RWFCTGDIG  H+DG L I+DRKKDLVKLQ GEY+SL KVE A+  C FV+  C YA S +++VI FI P  K+L  +A +  I E   ++IC    +   +L+E+++      L+R EIP  VH+    WTP+TGLVTDA KLKRK ++  ++ +I+ MY  
Sbjct:    3 LSAVLLFPVHAV-VWLYSLLSFLPWYFLTGAQEKKALA----------KRLKSKSTSDQPDGPYRSTDRFESLVTVDFPGKDTLDKLFDYAVERFGKLDCLGTREVLSEDNETQPNGKVFKKLILGEYKWKSYEELDIEVSHFGSGLAALGQQPK-STIAIFCETRAEWMITAQACFRRNFPLVTFYATLGEDAVAYGLNESGVTHLVTSIELLETKLKSVLLQIPNLKHIIYVDNKSMNTAG--------------FPQGIQIHSMHSVEELGA-----KPENLNRPIVKPVPSDLAVIMYTSGSTGRPKGVMIIHSNLIGGMAGQCERIPGLGPKDTYIAYLPLAHVLEMTAEISCVTYGCRIGYSSPQTLSDQSTKIKKGSKGDCSVLKPSLMAAVPEIMDRINKNVMSKVQEMSCVQRTLFKLAYDYKLEQIKKGYDAPLCN-ILFKKVRALLGGNVRMMLSGGAPLSPATQRFMNICFCCPVGQGYGLTETCGAGTITEVADYSTGRVGAPLICCEVKLKDWVEGGYTKRDKPHPRGEILIGGPNVTMGYYRTEGCNNDDFFVDQNGQRWFCTGDIGEMHADGCLQIVDRKKDLVKLQAGEYVSLGKVESALKNCSFVDNICAYANSDQNYVISFIVPNQKKLTALANQKGI-EGTWEEICNHPVMEREVLREIKEVANSIKLQRFEIPVMVHLSPEPWTPETGLVTDAFKLKRKELKNHYLNDIERMYGH 705

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Query:  144 MSILIPITVNALKLWTY-VYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYNR 2342
            +S ++   V+A+ +W Y +  F  W F    Q K  L+          + L     +D P  P+R+ + F  LV    PG +TLD L   A   +     LGTR+++  + E QPN K+FKKL   +Y W++Y ++D  +++FG GL  L  +P  S I IF ETR+EWMI AQ CFR N P++T Y+TLG++A+ +G+ ES  T ++TS EL+  KL  ++ +  ++KHII+++N      G               P GI +H M SVE +G+       E+L+  + KP    LA+IMYTSGST +PKGVM+ H NL+  MAGQ E +P +   D YI YLPLAHVLE+  E   +++G  +GYSSP TL+D+++KIK  +KGD S LKP++MA VP I+DRI K+V  K  +   +   +F+ AY+YK+ ++ +GY +   + ++F K++ LLGG VR MLSGGAPLS   Q F+NICFC P+ QGYGLTETCGAGT+    D    +VG PL   ++++++W EG Y+  D+P PRGEILIGG  VT+GY++      +DFFVD++G RWFCTGDIG  H+DG L I+DRKKDLVKLQ GEY+SL KVE A+  C FV+  C YA S +++VI FI P  K+L  +A +  I E   ++IC    +   +L+E+++      L+R EIP  VH+    WTP+TGLVTDA KLKRK ++  ++ +I+ MY  
Sbjct:    3 LSAVLLFPVHAV-VWLYSLLSFLPWYFLTGAQEKKALA----------KRLKSKSTSDQPDGPYRSTDRFESLVTVDFPGKDTLDKLFDYAVERFGKLDCLGTREVLSEDNETQPNGKVFKKLILGEYKWKSYEELDIEVSHFGSGLAALGQQPK-STIAIFCETRAEWMITAQACFRRNFPLVTFYATLGEDAVAYGLNESGVTHLVTSIELLETKLKSVLLQIPNLKHIIYVDNKSMNTAG--------------FPQGIQIHSMHSVEELGA-----KPENLNRPIVKPVPSDLAVIMYTSGSTGRPKGVMIIHSNLIGGMAGQCERIPGLGPKDTYIAYLPLAHVLEMTAEISCVTYGCRIGYSSPQTLSDQSTKIKKGSKGDCSVLKPSLMAAVPEIMDRINKNVMSKVQEMSCVQRTLFKLAYDYKLEQIKKGYDAPLCN-ILFKKVRALLGGNVRMMLSGGAPLSPATQRFMNICFCCPVGQGYGLTETCGAGTITEVADYSTGRVGAPLICCEVKLKDWVEGGYTKRDKPHPRGEILIGGPNVTMGYYRTEGCNNDDFFVDQNGQRWFCTGDIGEMHADGCLQIVDRKKDLVKLQAGEYVSLGKVESALKNCSFVDNICAYANSDQNYVISFIVPNQKKLTALANQKGI-EGTWEEICNHPVMEREVLREIKEVANSIKLQRFEIPVMVHLSPEPWTPETGLVTDAFKLKRKELKNHYLNDIERMYGH 705

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Xenopus
Match: acsl3 (acyl-CoA synthetase long chain family member 3 [Source:NCBI gene;Acc:100036652])

HSP 1 Score: 632.869 bits (1631), Expect = 0.000e+0
Identity = 327/687 (47.60%), Postives = 447/687 (65.07%), Query Frame = 3

Query:  285 RVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            RV AK +  D P+  +R+++SF+ L +++  GC+TLD +      L+ +K  LGTR+++  E E QPN K+FKK+   +Y W +Y +     + FG GL  L  KP  S I IF ETR+EW+I+AQ CF YN P++TLY+TLG  AI HG+ E+E T +ITS+EL+  KL  I+ +   ++HII +     +G G  +     T F    P  IT+H MG+V+  G+ +   +   L++    P    +A+IMYTSGST  PKGVM++H NL++ + G  + +P +   D YIGYLPLAHVLEL  E V LS G  VGYSSP TL+D+++KIK  +KGD S L+PT+MA VP I+DRI K+V  K     P+   +F  AY YKM ++ RGY +   D++VF K++KLLGGK+R +LSGGAPLS   Q F+NICFC P+ QGYGLTETCGAGT+    D    +VG PL   +I+++ W+EG Y   D+P PRGEILIGG  VT+GY+K   KT+EDFFVD++G RWFCTGDIG FHSDG L IIDRKKDLVKLQ GEY+SL KVE A+   P V+  C YA S + +VI F+ P  K+LL++AEK    +   + +C    +   +LK L ++     LE+ E+P K+ +    WTP+TGLVTDA KLKRK ++  +  +I+ MY 
Sbjct:    8 RVKAKSVA-DKPESAYRSVDSFHSLASSLYTGCDTLDKVFKHGTELFKHKDCLGTREVLSEEDEVQPNGKVFKKVILGRYNWMSYEEAYLFASQFGSGLAALGQKPR-SNIAIFCETRAEWIISAQACFMYNFPLVTLYATLGGPAIAHGLAETEVTHIITSKELLQTKLKAILPQVPLLRHIILV-----DGMGAQQ-----TDF----PKNITVHSMGAVQAFGAKQS--NLSVLNTQRSFPEARDIAVIMYTSGSTGIPKGVMISHSNLISGITGMAQRIPNLGATDTYIGYLPLAHVLELSAELVCLSRGCRVGYSSPQTLSDQSTKIKKGSKGDASELQPTLMAAVPEIMDRIYKNVMSKVASMSPVQKGLFSLAYNYKMKQISRGYSTPVCDRVVFWKVRKLLGGKLRVLLSGGAPLSAATQRFMNICFCCPVGQGYGLTETCGAGTITEVWDYSTGRVGAPLVCCEIKLKNWEEGGYYNTDRPCPRGEILIGGPNVTMGYYKNKAKTREDFFVDENGQRWFCTGDIGEFHSDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPIVDNICAYANSDQSYVIGFVVPNQKELLKLAEKKG-AQGCWESLCNNSVMEREVLKVLAESPAAATLEKFEVPVKIRLVSEPWTPETGLVTDAFKLKRKELKSHYQEDIERMYG 675

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Xenopus
Match: acsl3 (acyl-CoA synthetase long chain family member 3 [Source:NCBI gene;Acc:100036652])

HSP 1 Score: 630.172 bits (1624), Expect = 0.000e+0
Identity = 326/686 (47.52%), Postives = 446/686 (65.01%), Query Frame = 3

Query:  288 VLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            V+AK +  D P+  +R+++SF+ L +++  GC+TLD +      L+ +K  LGTR+++  E E QPN K+FKK+   +Y W +Y +     + FG GL  L  KP  S I IF ETR+EW+I+AQ CF YN P++TLY+TLG  AI HG+ E+E T +ITS+EL+  KL  I+ +   ++HII ++     G G  +     T F    P  IT+H MG+V+  G+       + L++    P    +A+IMYTSGST  PKGVM++H NL++ + G  + +P +   D YIGYLPLAHVLEL  E V LS G  VGYSSP TL+D+++KIK  +KGD S L+PT+MA VP I+DRI K+V  K     P+   +F  AY YKM ++ RGY +   D++VF K++KLLGGK+R +LSGGAPLS   Q F+NICFC P+ QGYGLTETCGAGT+    D    +VG PL   +I+++ W+EG Y   D+P PRGEILIGG  VT+GY+K   KT+EDFFVD++G RWFCTGDIG FHSDG L IIDRKKDLVKLQ GEY+SL KVE A+   P V+  C YA S + +VI F+ P  K+LL++AEK    +   + +C    +   +LK L ++     LE+ E+P K+ +    WTP+TGLVTDA KLKRK ++  +  +I+ MY 
Sbjct:    6 VVAKSVA-DKPESAYRSVDSFHSLASSLYTGCDTLDKVFKHGTELFKHKDCLGTREVLSEEDEVQPNGKVFKKVILGRYNWMSYEEAYLFASQFGSGLAALGQKPR-SNIAIFCETRAEWIISAQACFMYNFPLVTLYATLGGPAIAHGLAETEVTHIITSKELLQTKLKAILPQVPLLRHIILVD-----GMGAQQ-----TDF----PKNITVHSMGAVQAFGA-------KQLNTQRSFPEARDIAVIMYTSGSTGIPKGVMISHSNLISGITGMAQRIPNLGATDTYIGYLPLAHVLELSAELVCLSRGCRVGYSSPQTLSDQSTKIKKGSKGDASELQPTLMAAVPEIMDRIYKNVMSKVASMSPVQKGLFSLAYNYKMKQISRGYSTPVCDRVVFWKVRKLLGGKLRVLLSGGAPLSAATQRFMNICFCCPVGQGYGLTETCGAGTITEVWDYSTGRVGAPLVCCEIKLKNWEEGGYYNTDRPCPRGEILIGGPNVTMGYYKNKAKTREDFFVDENGQRWFCTGDIGEFHSDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPIVDNICAYANSDQSYVIGFVVPNQKELLKLAEKKG-AQGCWESLCNNSVMEREVLKVLAESPAAATLEKFEVPVKIRLVSEPWTPETGLVTDAFKLKRKELKSHYQEDIERMYG 667

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Xenopus
Match: acsl3 (acyl-CoA synthetase long chain family member 3 [Source:NCBI gene;Acc:100036652])

HSP 1 Score: 629.402 bits (1622), Expect = 0.000e+0
Identity = 339/749 (45.26%), Postives = 465/749 (62.08%), Query Frame = 3

Query:  141 RMSILIPITVNALKLWTYVYEFFTWPFYYI-FQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPT-------------ILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            R+ I+I   +N + L  Y+  FF W +  + F     LS          RV AK +  D P+  +R+++SF+ L +++  GC+TLD +      L+ +K  LGTR+++  E E QPN K+FKK+   +Y W +Y +     + FG GL  L  KP  S I IF ETR+EW+I+AQ CF YN P++TLY+TLG  AI HG+ E+E T +ITS+EL+  KL  I+ +   ++HII +     +G G  +     T F    P  IT+H MG+V+  G+       + L++    P    +A+IMYTSGST  PKGVM++H NL++ + G  + +P +   D YIGYLPLAHVLEL  E V LS G  VGYSSP TL+D+++KIK  +KGD S L+PT+MA VP              I+DRI K+V  K     P+   +F  AY YKM ++ RGY +   D++VF K++KLLGGK+R +LSGGAPLS   Q F+NICFC P+ QGYGLTETCGAGT+    D    +VG PL   +I+++ W+EG Y   D+P PRGEILIGG  VT+GY+K   KT+EDFFVD++G RWFCTGDIG FHSDG L IIDRKKDLVKLQ GEY+SL KVE A+   P V+  C YA S + +VI F+ P  K+LL++AEK    +   + +C    +   +LK L ++     LE+ E+P K+ +    WTP+TGLVTDA KLKRK ++  +  +I+ MY 
Sbjct:    3 RIVIVIKEDMNPVLL--YLLHFFMWLYGVVTFVPWFLLSGARDRQARASRVKAKSVA-DKPESAYRSVDSFHSLASSLYTGCDTLDKVFKHGTELFKHKDCLGTREVLSEEDEVQPNGKVFKKVILGRYNWMSYEEAYLFASQFGSGLAALGQKPR-SNIAIFCETRAEWIISAQACFMYNFPLVTLYATLGGPAIAHGLAETEVTHIITSKELLQTKLKAILPQVPLLRHIILV-----DGMGAQQ-----TDF----PKNITVHSMGAVQAFGA-------KQLNTQRSFPEARDIAVIMYTSGSTGIPKGVMISHSNLISGITGMAQRIPNLGATDTYIGYLPLAHVLELSAELVCLSRGCRVGYSSPQTLSDQSTKIKKGSKGDASELQPTLMAAVPVSSSPDNFCKKSVEIMDRIYKNVMSKVASMSPVQKGLFSLAYNYKMKQISRGYSTPVCDRVVFWKVRKLLGGKLRVLLSGGAPLSAATQRFMNICFCCPVGQGYGLTETCGAGTITEVWDYSTGRVGAPLVCCEIKLKNWEEGGYYNTDRPCPRGEILIGGPNVTMGYYKNKAKTREDFFVDENGQRWFCTGDIGEFHSDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPIVDNICAYANSDQSYVIGFVVPNQKELLKLAEKKG-AQGCWESLCNNSVMEREVLKVLAESPAAATLEKFEVPVKIRLVSEPWTPETGLVTDAFKLKRKELKSHYQEDIERMYG 725

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Xenopus
Match: acsl3 (acyl-CoA synthetase long chain family member 3 [Source:NCBI gene;Acc:100036652])

HSP 1 Score: 582.793 bits (1501), Expect = 0.000e+0
Identity = 304/638 (47.65%), Postives = 406/638 (63.64%), Query Frame = 3

Query:  192 YVYEFFTWPFYYI-FQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEK 2096
            Y+  FF W +  + F     LS          RV AK +  D P+  +R+++SF+ L +++  GC+TLD +      L+ +K  LGTR+++  E E QPN K+FKK+   +Y W +Y +     + FG GL  L  KP  S I IF ETR+EW+I+AQ CF YN P++TLY+TLG  AI HG+ E+E T +ITS+EL+  KL  I+ +   ++HII +     +G G  +     T F    P  IT+H MG+                + +   P    +A+IMYTSGST  PKGVM++H NL++ + G  + +P +   D YIGYLPLAHVLEL  E V LS G  VGYSSP TL+D+++KIK  +KGD S L+PT+MA VP I+DRI K+V  K     P+   +F  AY YKM ++ RGY +   D++VF K++KLLGGK+R +LSGGAPLS   Q F+NICFC P+ QGYGLTETCGAGT+    D    +VG PL   +I+++ W+EG Y   D+P PRGEILIGG  VT+GY+K   KT+EDFFVD++G RWFCTGDIG FHSDG L IIDRKKDLVKLQ GEY+SL KVE A+   P V+  C YA S + +VI F+ P  K+LL++AEK
Sbjct:   13 YLLHFFMWLYGVVTFVPWFLLSGARDRQARASRVKAKSVA-DKPESAYRSVDSFHSLASSLYTGCDTLDKVFKHGTELFKHKDCLGTREVLSEEDEVQPNGKVFKKVILGRYNWMSYEEAYLFASQFGSGLAALGQKPR-SNIAIFCETRAEWIISAQACFMYNFPLVTLYATLGGPAIAHGLAETEVTHIITSKELLQTKLKAILPQVPLLRHIILV-----DGMGAQQ-----TDF----PKNITVHSMGAP---------------NLHFKNPGARDIAVIMYTSGSTGIPKGVMISHSNLISGITGMAQRIPNLGATDTYIGYLPLAHVLELSAELVCLSRGCRVGYSSPQTLSDQSTKIKKGSKGDASELQPTLMAAVPEIMDRIYKNVMSKVASMSPVQKGLFSLAYNYKMKQISRGYSTPVCDRVVFWKVRKLLGGKLRVLLSGGAPLSAATQRFMNICFCCPVGQGYGLTETCGAGTITEVWDYSTGRVGAPLVCCEIKLKNWEEGGYYNTDRPCPRGEILIGGPNVTMGYYKNKAKTREDFFVDENGQRWFCTGDIGEFHSDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPIVDNICAYANSDQSYVIGFVVPNQKELLKLAEK 619

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Xenopus
Match: acsl3 (acyl-CoA synthetase long chain family member 3 [Source:NCBI gene;Acc:100036652])

HSP 1 Score: 556.984 bits (1434), Expect = 0.000e+0
Identity = 287/579 (49.57%), Postives = 383/579 (66.15%), Query Frame = 3

Query:  609 SRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            S I IF ETR+EW+I+AQ CF YN P++TLY+TLG  AI HG+ E+E T +ITS+EL+  KL  I+ +   ++HII +     +G G  +     T F    P  IT+H MG+V+  G+       + L++    P    +A+IMYTSGST  PKGVM++H NL++ + G  + +P +   D YIGYLPLAHVLEL  E V LS G  VGYSSP TL+D+++KIK  +KGD S L+PT+MA VP I+DRI K+V  K     P+   +F  AY YKM ++ RGY +   D++VF K++KLLGGK+R +LSGGAPLS   Q F+NICFC P+ QGYGLTETCGAGT+    D    +VG PL   +I+++ W+EG Y   D+P PRGEILIGG  VT+GY+K   KT+EDFFVD++G RWFCTGDIG FHSDG L IIDRKKDLVKLQ GEY+SL KVE A+   P V+  C YA S + +VI F+ P  K+LL++AEK    +   + +C    +   +LK L ++     LE+ E+P K+ +    WTP+TGLVTDA KLKRK ++  +  +I+ MY 
Sbjct:    6 SNIAIFCETRAEWIISAQACFMYNFPLVTLYATLGGPAIAHGLAETEVTHIITSKELLQTKLKAILPQVPLLRHIILV-----DGMGAQQ-----TDF----PKNITVHSMGAVQAFGA-------KQLNTQRSFPEARDIAVIMYTSGSTGIPKGVMISHSNLISGITGMAQRIPNLGATDTYIGYLPLAHVLELSAELVCLSRGCRVGYSSPQTLSDQSTKIKKGSKGDASELQPTLMAAVPEIMDRIYKNVMSKVASMSPVQKGLFSLAYNYKMKQISRGYSTPVCDRVVFWKVRKLLGGKLRVLLSGGAPLSAATQRFMNICFCCPVGQGYGLTETCGAGTITEVWDYSTGRVGAPLVCCEIKLKNWEEGGYYNTDRPCPRGEILIGGPNVTMGYYKNKAKTREDFFVDENGQRWFCTGDIGEFHSDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPIVDNICAYANSDQSYVIGFVVPNQKELLKLAEKKG-AQGCWESLCNNSVMEREVLKVLAESPAAATLEKFEVPVKIRLVSEPWTPETGLVTDAFKLKRKELKSHYQEDIERMYG 562

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Mouse
Match: Acsl4 (acyl-CoA synthetase long-chain family member 4 [Source:MGI Symbol;Acc:MGI:1354713])

HSP 1 Score: 654.44 bits (1687), Expect = 0.000e+0
Identity = 338/731 (46.24%), Postives = 472/731 (64.57%), Query Frame = 3

Query:  150 ILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            IL+P+ +    L T        P+Y++  +K         N    R+ AKP  +D P  P+R++  F+ L    IPG +TLD L   A   +  K +LGTR+++  E E QPN K+FKKL    Y W  Y +++ R+ NFG GL  L +KP  + I IF ETR+EWMIAAQTCF+YN P++TLY+TLG EA+VHG+ ESEA+ +ITS EL+  KL   +   + VKHII+++N        N+A+          P+G+ +H M SVE +G+       E+L     +PT   +A++MYTSGST +PKGVMM H NL+  M GQ E +P +   D YIGYLPLAHVLEL  E    ++G  +GYSSPLTL+D++SKIK  +KGD + LKPT+MA VP I+DRI K+V  K  +   +   +F+  Y+YK+ ++ +GY +   + ++F K+K LLGG VR MLSGGAPLS +   F+N+CFC PI QGYGLTE+CGAGT+    D    +VG PL   +I++++W+EG Y+  D+P PRGEI+IGG+ +++GYFK  +KT ED+ VD++G RWFCTGDIG FH DG L IIDRKKDLVKLQ GEY+SL KVE A+  CP ++  C +A+S + +VI F+ P  K+L  +A++  +E   +  IC    + + +LKE+++A     LER EIP KV +    WTP+TGLVTDA KLKRK ++  ++ +I+ MY
Sbjct:   11 ILLPVHL----LITIYSALIFIPWYFLTNAK-------KKNAMAKRIKAKP-TSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVAKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWINYLEVNCRVNNFGSGLTALGLKPK-NTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGREAVVHGLNESEASYLITSVELLESKLKAALVDINCVKHIIYVDNKTI-----NRAE---------YPEGLEIHSMQSVEELGA-----KPENLSVPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYVQKTLFKIGYDYKLEQIKKGYDAPLCNLILFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTVHDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYCVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKKLTLLAQQKGVEGSWVD-ICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELKNHYLKDIERMY 708

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Query:  150 ILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            IL+P+ +    L T        P+Y++  +K         N    R+ AKP  +D P  P+R++  F+ L    IPG +TLD L   A   +  K +LGTR+++  E E QPN K+FKKL    Y W  Y +++ R+ NFG GL  L +KP  + I IF ETR+EWMIAAQTCF+YN P++TLY+TLG EA+VHG+ ESEA+ +ITS EL+  KL   +   + VKHII+++N        N+A+          P+G+ +H M SVE +G+       E+L     +PT   +A++MYTSGST +PKGVMM H NL+  M GQ E +P +   D YIGYLPLAHVLEL  E    ++G  +GYSSPLTL+D++SKIK  +KGD + LKPT+MA VP I+DRI K+V  K  +   +   +F+  Y+YK+ ++ +GY +   + ++F K+K LLGG VR MLSGGAPLS +   F+N+CFC PI QGYGLTE+CGAGT+    D    +VG PL   +I++++W+EG Y+  D+P PRGEI+IGG+ +++GYFK  +KT ED+ VD++G RWFCTGDIG FH DG L IIDRKKDLVKLQ GEY+SL KVE A+  CP ++  C +A+S + +VI F+ P  K+L  +A++  +E   +  IC    + + +LKE+++A     LER EIP KV +    WTP+TGLVTDA KLKRK ++  ++ +I+ MY
Sbjct:   11 ILLPVHL----LITIYSALIFIPWYFLTNAK-------KKNAMAKRIKAKP-TSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVAKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWINYLEVNCRVNNFGSGLTALGLKPK-NTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGREAVVHGLNESEASYLITSVELLESKLKAALVDINCVKHIIYVDNKTI-----NRAE---------YPEGLEIHSMQSVEELGA-----KPENLSVPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYVQKTLFKIGYDYKLEQIKKGYDAPLCNLILFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTVHDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYCVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKKLTLLAQQKGVEGSWVD-ICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELKNHYLKDIERMY 708

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Mouse
Match: Acsl4 (acyl-CoA synthetase long-chain family member 4 [Source:MGI Symbol;Acc:MGI:1354713])

HSP 1 Score: 650.973 bits (1678), Expect = 0.000e+0
Identity = 329/686 (47.96%), Postives = 456/686 (66.47%), Query Frame = 3

Query:  285 RVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            R+ AKP  +D P  P+R++  F+ L    IPG +TLD L   A   +  K +LGTR+++  E E QPN K+FKKL    Y W  Y +++ R+ NFG GL  L +KP  + I IF ETR+EWMIAAQTCF+YN P++TLY+TLG EA+VHG+ ESEA+ +ITS EL+  KL   +   + VKHII+++N        N+A+          P+G+ +H M SVE +G+       E+L     +PT   +A++MYTSGST +PKGVMM H NL+  M GQ E +P +   D YIGYLPLAHVLEL  E    ++G  +GYSSPLTL+D++SKIK  +KGD + LKPT+MA VP I+DRI K+V  K  +   +   +F+  Y+YK+ ++ +GY +   + ++F K+K LLGG VR MLSGGAPLS +   F+N+CFC PI QGYGLTE+CGAGT+    D    +VG PL   +I++++W+EG Y+  D+P PRGEI+IGG+ +++GYFK  +KT ED+ VD++G RWFCTGDIG FH DG L IIDRKKDLVKLQ GEY+SL KVE A+  CP ++  C +A+S + +VI F+ P  K+L  +A++  +E   +  IC    + + +LKE+++A     LER EIP KV +    WTP+TGLVTDA KLKRK ++  ++ +I+ MY
Sbjct:    4 RIKAKPT-SDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVAKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWINYLEVNCRVNNFGSGLTALGLKPK-NTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGREAVVHGLNESEASYLITSVELLESKLKAALVDINCVKHIIYVDNKTI-----NRAE---------YPEGLEIHSMQSVEELGA-----KPENLSVPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYVQKTLFKIGYDYKLEQIKKGYDAPLCNLILFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTVHDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYCVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKKLTLLAQQKGVEGSWVD-ICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELKNHYLKDIERMY 667

[Close]

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Mouse
Match: Acsl4 (acyl-CoA synthetase long-chain family member 4 [Source:MGI Symbol;Acc:MGI:1354713])

HSP 1 Score: 650.973 bits (1678), Expect = 0.000e+0
Identity = 329/686 (47.96%), Postives = 456/686 (66.47%), Query Frame = 3

Query:  285 RVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            R+ AKP  +D P  P+R++  F+ L    IPG +TLD L   A   +  K +LGTR+++  E E QPN K+FKKL    Y W  Y +++ R+ NFG GL  L +KP  + I IF ETR+EWMIAAQTCF+YN P++TLY+TLG EA+VHG+ ESEA+ +ITS EL+  KL   +   + VKHII+++N        N+A+          P+G+ +H M SVE +G+       E+L     +PT   +A++MYTSGST +PKGVMM H NL+  M GQ E +P +   D YIGYLPLAHVLEL  E    ++G  +GYSSPLTL+D++SKIK  +KGD + LKPT+MA VP I+DRI K+V  K  +   +   +F+  Y+YK+ ++ +GY +   + ++F K+K LLGG VR MLSGGAPLS +   F+N+CFC PI QGYGLTE+CGAGT+    D    +VG PL   +I++++W+EG Y+  D+P PRGEI+IGG+ +++GYFK  +KT ED+ VD++G RWFCTGDIG FH DG L IIDRKKDLVKLQ GEY+SL KVE A+  CP ++  C +A+S + +VI F+ P  K+L  +A++  +E   +  IC    + + +LKE+++A     LER EIP KV +    WTP+TGLVTDA KLKRK ++  ++ +I+ MY
Sbjct:    4 RIKAKPT-SDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVAKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWINYLEVNCRVNNFGSGLTALGLKPK-NTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGREAVVHGLNESEASYLITSVELLESKLKAALVDINCVKHIIYVDNKTI-----NRAE---------YPEGLEIHSMQSVEELGA-----KPENLSVPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYVQKTLFKIGYDYKLEQIKKGYDAPLCNLILFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTVHDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYCVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKKLTLLAQQKGVEGSWVD-ICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELKNHYLKDIERMY 667

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Mouse
Match: Acsl3 (acyl-CoA synthetase long-chain family member 3 [Source:MGI Symbol;Acc:MGI:1921455])

HSP 1 Score: 617.846 bits (1592), Expect = 0.000e+0
Identity = 327/731 (44.73%), Postives = 450/731 (61.56%), Query Frame = 3

Query:  159 PITVNALKLWTYVYEFFTW-PFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICF-CPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYNR 2342
            PI +  +     +Y   T+ PFY++ +SK              ++ AKP+ +  P   +R+INS +GL + + PGC+TLD + + A N + NK  LGTR+++  E E QPN K+FKK+    Y W +Y D+  R  +FG GL  L  KP  + I IF ETR+EWMIAAQ CF YN  ++TLY+TLG  AIVHG+ E+E T +ITS+EL+  KL  I+     ++HII +              D      +  P G+ +H M +V+ +G   +   E+   S   KP    +A+IMYTSGST  PKGVM++H N++ ++ G    +P +  +D YIGYLPLAHVLEL  E V LSHG  +GYSSP TL D++SKIK  +KGD S LKPT+MA VP I+DRI K+V  K  +       +F  AY YKM ++ +G  +   D+ VF  +++LLGG +R +L GGAPLS   Q F+NICF CP+ QGYGLTE+ GAGT+    D    +VG PL   +I+++ W+EG Y   D+P PRGEILIGG+ VT+GY+K   KTK DFF D++G RW CTGDIG F  DG L IIDRKKDLVKLQ GEY+SL KVE A+   P ++  C YA S   +VI F+ P  K+L ++A      +   +++C   E+ + +LK L +A +   LE+ EIP K+ +    WTP+TGLVTDA KLKRK ++  +  +I+ MY R
Sbjct:   20 PILLYFIHFIISLYTILTYIPFYFLCESK---------QEKPNQIKAKPV-SSKPDSAYRSINSVDGLASVLYPGCDTLDKVFMYAKNKFKNKRLLGTREILNEEDEIQPNGKIFKKVILGHYNWLSYEDVFIRALDFGNGLQMLGQKPK-ANIAIFCETRAEWMIAAQACFMYNFQLVTLYATLGGPAIVHGLNETEVTNIITSKELLQTKLKDIVSLVPRLRHIITV--------------DGKPPTWSEFPKGVIVHTMAAVQALGVKANV--EKKAHS---KPLPSDIAVIMYTSGSTGIPKGVMISHSNIIASITGMARRIPRLGEEDVYIGYLPLAHVLELSAELVCLSHGCRIGYSSPQTLADQSSKIKKGSKGDTSVLKPTLMAAVPEIMDRIYKNVMNKVNEMSAFQRNLFILAYNYKMEQISKGCSTPLCDRFVFRNVRRLLGGNIRLLLCGGAPLSATTQRFMNICFCCPVGQGYGLTESTGAGTITEVWDYNTGRVGAPLVCCEIKLKNWEEGGYFNTDKPHPRGEILIGGQNVTMGYYKNEAKTKTDFFEDENGQRWLCTGDIGEFDPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPLIDNICAYANSYHSYVIGFVVPNQKELTELARTKGF-KGTWEELCNSSEMENEVLKVLSEAAISASLEKFEIPLKIRLSPDPWTPETGLVTDAFKLKRKELKTHYQADIERMYGR 719

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. UniProt/SwissProt
Match: sp|O60488|ACSL4_HUMAN (Long-chain-fatty-acid--CoA ligase 4 OS=Homo sapiens OX=9606 GN=ACSL4 PE=1 SV=2)

HSP 1 Score: 656.366 bits (1692), Expect = 0.000e+0
Identity = 341/731 (46.65%), Postives = 472/731 (64.57%), Query Frame = 3

Query:  150 ILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            IL+P+ +    L T        P+Y++  +K         N    R+ AKP  +D P  P+R++  F+ L    IPG +TLD L   A + +  K +LGTR+++  E E QPN K+FKKL    Y W  Y +++ R+ NFG GL  L +KP  + I IF ETR+EWMIAAQTCF+YN P++TLY+TLG EA+VHG+ ESEA+ +ITS EL+  KL   +     VKHII+++N        NKA+          P+G  +H M SVE +GS     + E+L     +PT   +A++MYTSGST +PKGVMM H NL+  M GQ E +P +   D YIGYLPLAHVLEL  E    ++G  +GYSSPLTL+D++SKIK  +KGD + LKPT+MA VP I+DRI K+V  K  +   +   +F+  Y+YK+ ++ +GY +   + L+F K+K LLGG VR MLSGGAPLS +   F+N+CFC PI QGYGLTE+CGAGT+    D    +VG PL   +I++++W+EG Y+  D+P PRGEI+IGG+ +++GYFK  +KT ED+ VD++G RWFCTGDIG FH DG L IIDRKKDLVKLQ GEY+SL KVE A+  CP ++  C +A+S + +VI F+ P  K+L  +A++  +E   +  IC    + + +LKE+++A     LER EIP KV +    WTP+TGLVTDA KLKRK ++  ++ +I+ MY
Sbjct:   11 ILLPVHL----LITIYSALIFIPWYFLTNAK-------KKNAMAKRIKAKP-TSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVSKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWMNYLEVNRRVNNFGSGLTALGLKPK-NTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAI-----NKAE---------YPEGFEIHSMQSVEELGS-----NPENLGIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYIQKTLFKIGYDYKLEQIKKGYDAPLCNLLLFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTINDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYSVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKRLTLLAQQKGVEGTWVD-ICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELRNHYLKDIERMY 708

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. UniProt/SwissProt
Match: sp|Q9QUJ7|ACSL4_MOUSE (Long-chain-fatty-acid--CoA ligase 4 OS=Mus musculus OX=10090 GN=Acsl4 PE=1 SV=2)

HSP 1 Score: 654.44 bits (1687), Expect = 0.000e+0
Identity = 338/731 (46.24%), Postives = 472/731 (64.57%), Query Frame = 3

Query:  150 ILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            IL+P+ +    L T        P+Y++  +K         N    R+ AKP  +D P  P+R++  F+ L    IPG +TLD L   A   +  K +LGTR+++  E E QPN K+FKKL    Y W  Y +++ R+ NFG GL  L +KP  + I IF ETR+EWMIAAQTCF+YN P++TLY+TLG EA+VHG+ ESEA+ +ITS EL+  KL   +   + VKHII+++N        N+A+          P+G+ +H M SVE +G+       E+L     +PT   +A++MYTSGST +PKGVMM H NL+  M GQ E +P +   D YIGYLPLAHVLEL  E    ++G  +GYSSPLTL+D++SKIK  +KGD + LKPT+MA VP I+DRI K+V  K  +   +   +F+  Y+YK+ ++ +GY +   + ++F K+K LLGG VR MLSGGAPLS +   F+N+CFC PI QGYGLTE+CGAGT+    D    +VG PL   +I++++W+EG Y+  D+P PRGEI+IGG+ +++GYFK  +KT ED+ VD++G RWFCTGDIG FH DG L IIDRKKDLVKLQ GEY+SL KVE A+  CP ++  C +A+S + +VI F+ P  K+L  +A++  +E   +  IC    + + +LKE+++A     LER EIP KV +    WTP+TGLVTDA KLKRK ++  ++ +I+ MY
Sbjct:   11 ILLPVHL----LITIYSALIFIPWYFLTNAK-------KKNAMAKRIKAKP-TSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVAKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWINYLEVNCRVNNFGSGLTALGLKPK-NTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGREAVVHGLNESEASYLITSVELLESKLKAALVDINCVKHIIYVDNKTI-----NRAE---------YPEGLEIHSMQSVEELGA-----KPENLSVPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYVQKTLFKIGYDYKLEQIKKGYDAPLCNLILFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTVHDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYCVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKKLTLLAQQKGVEGSWVD-ICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELKNHYLKDIERMY 708

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. UniProt/SwissProt
Match: sp|O35547|ACSL4_RAT (Long-chain-fatty-acid--CoA ligase 4 OS=Rattus norvegicus OX=10116 GN=Acsl4 PE=1 SV=2)

HSP 1 Score: 652.899 bits (1683), Expect = 0.000e+0
Identity = 337/731 (46.10%), Postives = 471/731 (64.43%), Query Frame = 3

Query:  150 ILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            +L+P+ +    L T        P+Y++  +K         N    R+ AKP  +D P  P+R++  F+ L    IPG +TLD L   A   +  K +LGTR+++  E E QPN K+FKKL    Y W  Y +++ R+ NFG GL  L +KP  + I IF ETR+EWMIAAQTCF+YN P++TLY+TLG EA+VHG+ ESEA+ +ITS EL+  KL   +   + VKHII+++N        N+A+          P+G+ +H M SVE +GS       E+      +PT   +A++MYTSGST +PKGVMM H NL+  M GQ E +P +   D YIGYLPLAHVLEL  E    ++G  +GYSSPLTL+D++SKIK  +KGD + LKPT+MA VP I+DRI K+V  K  +   +   +F+  Y+YK+ ++ +GY +   + ++F K+K LLGG VR MLSGGAPLS +   F+N+CFC PI QGYGLTE+CGAGT+    D    +VG PL   +I++++W+EG Y+  D+P PRGEI+IGG+ +++GYFK  +KT ED+ VD++G RWFCTGDIG FH DG L IIDRKKDLVKLQ GEY+SL KVE A+  CP ++  C +A+S + +VI F+ P  K+L  +A++  +E   +  IC    + + +LKE+++A     LER EIP KV +    WTP+TGLVTDA KLKRK ++  ++ +I+ MY
Sbjct:   11 VLLPVHL----LITIYSALIFIPWYFLTNAK-------KKNAMAKRIKAKPT-SDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVAKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWINYLEVNCRVNNFGSGLTALGLKPK-NTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKAALLDINCVKHIIYVDNKTI-----NRAE---------YPEGLEIHSMQSVEELGS-----KPENSSIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYIQKTLFKIGYDYKLEQIKKGYDAPLCNLILFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTVHDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYSVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKKLTLLAQQKGVEGSWVD-ICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELKNHYLKDIERMY 708

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. UniProt/SwissProt
Match: sp|O95573|ACSL3_HUMAN (Long-chain-fatty-acid--CoA ligase 3 OS=Homo sapiens OX=9606 GN=ACSL3 PE=1 SV=3)

HSP 1 Score: 630.172 bits (1624), Expect = 0.000e+0
Identity = 332/731 (45.42%), Postives = 451/731 (61.70%), Query Frame = 3

Query:  159 PITVNALKLWTYVYEFFTW-PFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICF-CPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYNR 2342
            PI +  +     +Y   T+ PFY+  +S+   S+         R+ AKP+ N  P   +R++NS +GL + + PGC+TLD +   A N + NK  LGTR+++  E E QPN K+FKK+   +Y W +Y D+  R  NFG GL  L  KP  + I IF ETR+EWMIAAQ CF YN  ++TLY+TLG  AIVH + E+E T +ITS+EL+  KL  I+     ++HII +              D      +  P GI +H M +VE +G+     ++        KP    +A+IMYTSGST  PKGVM++H N++  + G  E +P +  +D YIGYLPLAHVLEL  E V LSHG  +GYSSP TL D++SKIK  +KGD S LKPT+MA VP I+DRI K+V  K ++       +F  AY YKM ++ +G  +   D  VF K++ LLGG +R +L GGAPLS   Q F+NICF CP+ QGYGLTE+ GAGT+    D    +VG PL   +I+++ W+EG Y   D+P PRGEILIGG+ VT+GY+K   KTK DFF D++G RW CTGDIG F  DG L IIDRKKDLVKLQ GEY+SL KVE A+   P V+  C YA S   +VI F+ P  K+L ++A K  + +   +++C   E+ + +LK L +A +   LE+ EIP K+ +    WTP+TGLVTDA KLKRK ++  +  +I+ MY R
Sbjct:   20 PILLYFIHFLISLYTILTYIPFYFFSESRQEKSN---------RIKAKPV-NSKPDSAYRSVNSLDGLASVLYPGCDTLDKVFTYAKNKFKNKRLLGTREVLNEEDEVQPNGKIFKKVILGQYNWLSYEDVFVRAFNFGNGLQMLGQKPK-TNIAIFCETRAEWMIAAQACFMYNFQLVTLYATLGGPAIVHALNETEVTNIITSKELLQTKLKDIVSLVPRLRHIITV--------------DGKPPTWSEFPKGIIVHTMAAVEALGAKASMENQPH-----SKPLPSDIAVIMYTSGSTGLPKGVMISHSNIIAGITGMAERIPELGEEDVYIGYLPLAHVLELSAELVCLSHGCRIGYSSPQTLADQSSKIKKGSKGDTSMLKPTLMAAVPEIMDRIYKNVMNKVSEMSSFQRNLFILAYNYKMEQISKGRNTPLCDSFVFRKVRSLLGGNIRLLLCGGAPLSATTQRFMNICFCCPVGQGYGLTESAGAGTISEVWDYNTGRVGAPLVCCEIKLKNWEEGGYFNTDKPHPRGEILIGGQSVTMGYYKNEAKTKADFFEDENGQRWLCTGDIGEFEPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPLVDNICAYANSYHSYVIGFVVPNQKELTELARKKGL-KGTWEELCNSCEMENEVLKVLSEAAISASLEKFEIPVKIRLSPEPWTPETGLVTDAFKLKRKELKTHYQADIERMYGR 719

[Close]

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. UniProt/SwissProt
Match: sp|Q5R668|ACSL3_PONAB (Long-chain-fatty-acid--CoA ligase 3 OS=Pongo abelii OX=9601 GN=ACSL3 PE=2 SV=2)

HSP 1 Score: 628.246 bits (1619), Expect = 0.000e+0
Identity = 331/731 (45.28%), Postives = 451/731 (61.70%), Query Frame = 3

Query:  159 PITVNALKLWTYVYEFFTW-PFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICF-CPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYNR 2342
            PI +  +     +Y   T+ PFY+  +S+   S+         R+ AKP+ N  P   +R++NS +GL + + PGC+TLD +   A N + NK  LGTR+++  E E QPN K+FKK+   +Y W +Y D+  R  NFG GL  L  KP  + I IF ETR+EWMIAAQ CF YN  ++TLY+TLG  AIVH + E+E T +ITS+EL+  KL  I+     ++HII +              D      +  P GI +H M +VE +G+     ++        KP    +A+IMYTSGST  PKGVM++H N++  + G  E +P +  +D YIGYLPLAHVLEL  E V LSHG  +GYSSP TL D++SKIK  +KGD S LKPT+MA VP I+DRI K+V  K ++       +F  AY YKM ++ +G  +   +  VF K++ LLGG +R +L GGAPLS   Q F+NICF CP+ QGYGLTE+ GAGT+    D    +VG PL   +I+++ W+EG Y   D+P PRGEILIGG+ VT+GY+K   KTK DFF D++G RW CTGDIG F  DG L IIDRKKDLVKLQ GEY+SL KVE A+   P V+  C YA S   +VI F+ P  K+L ++A K  + +   +++C   E+ + +LK L +A +   LE+ EIP K+ +    WTP+TGLVTDA KLKRK ++  +  +I+ MY R
Sbjct:   20 PILLYFIHFLISLYTILTYIPFYFFSESRQEKSN---------RIKAKPV-NSKPDSAYRSVNSLDGLASVLYPGCDTLDKVFTYAKNKFKNKRLLGTREVLNEEDEVQPNGKIFKKVILGQYNWLSYEDVFVRAFNFGNGLQMLGQKPK-TNIAIFCETRAEWMIAAQACFMYNFQLVTLYATLGGPAIVHALNETEVTNIITSKELLQTKLKDIVSLVPRLRHIITV--------------DGKPPTWSEFPKGIIVHTMAAVEALGAKASMENQPH-----SKPLPSDIAVIMYTSGSTGLPKGVMISHSNIIAGITGMAERIPELGEEDVYIGYLPLAHVLELSAELVCLSHGCRIGYSSPQTLADQSSKIKKGSKGDTSMLKPTLMAAVPEIMDRIYKNVMNKVSEMSSFQRNLFILAYNYKMEQISKGRNTPLCNSFVFRKVRSLLGGNIRLLLCGGAPLSATTQRFMNICFCCPVGQGYGLTESAGAGTISEVWDYNTGRVGAPLVCCEIKLKNWEEGGYFNTDKPHPRGEILIGGQIVTMGYYKNEAKTKADFFEDENGQRWLCTGDIGEFEPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPLVDNICAYANSYHSYVIGFVVPNQKELTELARKKGL-KGTWEELCNSCEMENEVLKVLSEAAISASLEKFEIPVKIRLSPEPWTPETGLVTDAFKLKRKELKTHYQADIERMYGR 719

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. TrEMBL
Match: A0A267GRF3 (AMP-binding domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030308g1 PE=4 SV=1)

HSP 1 Score: 746.117 bits (1925), Expect = 0.000e+0
Identity = 382/741 (51.55%), Postives = 496/741 (66.94%), Query Frame = 3

Query:  144 MSILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHR--DDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICF-CPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIE------EKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            MSIL  +    ++  TYVY+F TWP YY   +   ++  NS      +V AK + ND P+ PWR + S + LV       +++ D++  A   +P+K  LGTR+L+  E E QPN ++FKKL F  Y+WETY +++ R+ NFGRGL  L MKP+  RIL+FAETR+EW I     FR+N P++TLYSTLGD+AIV+GI ++E  +VITS+ L+ K   ++ K   VKHII+M+N +F   GG+K   L     N    G+  H M SVE +G  K     +S  S    P    LA+IMYTSGST  PKGVM+THGNLM  +A   + +P   R  DD YIGYLPLAHVLEL  E   L+ G+P+GYSSPLTL+D ++KIK  TKGDIS LKPT+MA VP I+DRI+K+VWEK  D G +   +F WAY+YK +RM RG PS  +DK++F K+K LLGG+VR MLSGGAPLS E Q F+N+CF  PI+QGYGLTET GAGT+    D    + G PL   QI++R+W EG YS  DQP PRGE+LIGG  VTLGYFK PDKTKEDFFVD DG+RWFCTGDIG+F+ +G+L I+DRKKDLVKLQGGEYISL KVE A+    FVE  CVY +S +D  +  + PK  Q++Q+AEK+ I           + + K K +  A+LK+LQ     K +ER E+P +V++   QWTP++GLVTDALKLKR+ +Q+ F  EI  MY+
Sbjct:    1 MSILFAV----VRAVTYVYDFVTWPLYYFLYAPDEVAK-NSR-----KVRAKRLSND-PESPWRCVESMDKLVEKPTLAVDSMADMLRIAVAKYPSKRCLGTRELLSEEDERQPNGRVFKKLCFGDYHWETYEEVNIRVDNFGRGLANLGMKPT-KRILVFAETRAEWQICVNAAFRFNHPVVTLYSTLGDDAIVYGINQAEVDIVITSDSLVSKFKNLLPKISQVKHIIYMDNGVF---GGSKDPKLQLGEIN----GVQFHAMTSVERLG--KQQAAAKSTQSAASSPKSGDLAVIMYTSGSTGLPKGVMITHGNLMATIASAVDRIPQFGRRPDDTYIGYLPLAHVLELVAELACLTGGVPIGYSSPLTLSDVSNKIKKGTKGDISVLKPTLMAAVPAIMDRISKNVWEKVNDGGAVSKALFNWAYDYKKDRMLRGMPSPLLDKIIFNKVKYLLGGRVRVMLSGGAPLSTETQRFMNVCFGIPIVQGYGLTETTGAGTVQTVDDISTGRAGPPLACCQIRLRDWAEGGYSSRDQPCPRGEVLIGGNNVTLGYFKMPDKTKEDFFVDSDGIRWFCTGDIGLFNVNGTLEIVDRKKDLVKLQGGEYISLGKVETALGVSAFVEQLCVYGDSFKDHTVAIVVPKKTQVMQLAEKLGISGDTDATGDSYESLLKNKAIKDAVLKDLQSKAAGK-VERFELPTRVYLTSEQWTPESGLVTDALKLKRRQLQDHFQSEIDRMYS 719

[Close]

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. TrEMBL
Match: A0A267GYX3 (AMP-binding domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030601g1 PE=4 SV=1)

HSP 1 Score: 745.732 bits (1924), Expect = 0.000e+0
Identity = 381/741 (51.42%), Postives = 495/741 (66.80%), Query Frame = 3

Query:  144 MSILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHR--DDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICF-CPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIE------EKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            MSIL  +    ++  TYVY+F TWP YY   +   ++  NS      +V AK + ND P+ PWR + S + LV       +++ D++  A   +P+K  LGTR+L+  E E QPN ++FKKL F  Y+WETY +++ R+ NFGRGL  L MKP+  RIL+FAETR+EW I     FR+N P++TLYSTLGD+AIV+GI ++E  +VITS+ L+ K    + K   VKHII+M+N +F   GG+K   L     N    G+  H M SVE +G  K     +S  S    P    LA+IMYTSGST  P+GVM+THGNLM  +A   + +P   R  DD YIGYLPLAHVLEL  E   L+ G+P+GYSSPLTL+D ++KIK  TKGDIS LKPT+MA VP I+DRI+K+VWEK  D G +   +F WAY+YK +RM RG PS  +DK++F K+K LLGG+VR MLSGGAPLS E Q F+N+CF  PI+QGYGLTET GAGT+    D    + G PL   QI++R+W EG YS  DQP PRGE+LIGG  VTLGYFK PDKTKEDFFVD DG+RWFCTGDIG+F+ +G+L I+DRKKDLVKLQGGEYISL KVE A+    FVE  CVY +S +D  +  + PK  Q++Q+AEK+ I           + + K K +  A+LK+LQ     K +ER E+P +V++   QWTP++GLVTDALKLKR+ +Q+ F  EI  MY+
Sbjct:    1 MSILFAV----VRAVTYVYDFVTWPLYYFLYAPDEVAK-NSR-----KVRAKRLSND-PESPWRCVESMDKLVEKPTLAVDSMADMLRIAVAKYPSKRCLGTRELLSEEDERQPNGRVFKKLCFGDYHWETYEEVNIRVDNFGRGLANLGMKPT-KRILVFAETRAEWQICVNAAFRFNHPVVTLYSTLGDDAIVYGINQAEVDIVITSDSLVSKFKNFLPKISQVKHIIYMDNGVF---GGSKDPKLQLGEIN----GVQFHAMTSVERLG--KQQAAAKSTQSAASSPKSGDLAVIMYTSGSTGLPRGVMITHGNLMATIASAVDRIPQFGRRPDDTYIGYLPLAHVLELVAELACLTGGVPIGYSSPLTLSDVSNKIKKGTKGDISVLKPTLMAAVPAIMDRISKNVWEKVNDGGAVSKALFNWAYDYKKDRMLRGMPSPLLDKIIFNKVKSLLGGRVRVMLSGGAPLSTETQRFMNVCFGIPIVQGYGLTETTGAGTVQTVDDISTGRAGPPLACCQIRLRDWAEGGYSSRDQPCPRGEVLIGGNNVTLGYFKMPDKTKEDFFVDSDGIRWFCTGDIGLFNVNGTLEIVDRKKDLVKLQGGEYISLGKVETALGVSAFVEQLCVYGDSFKDHTVAIVVPKKTQVMQLAEKLGISGDTDATGDSYESLLKNKAIKDAVLKDLQSKAAGK-VERFELPTRVYLTSEQWTPESGLVTDALKLKRRQLQDHFQSEIDRMYS 719

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. TrEMBL
Match: A0A1I8GP46 (AMP-binding domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 712.99 bits (1839), Expect = 0.000e+0
Identity = 366/741 (49.39%), Postives = 478/741 (64.51%), Query Frame = 3

Query:  144 MSILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHR--DDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICF-CPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIE------EKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            MSIL  +    ++  TYVY+F TWP YY   +   ++  NS      +V AK + ND P+ PWR + S + LV       +++ D++  A   +P+K  LGTR+L+  E E QPN ++FKKL F  Y+WETY +++ R+ NFGRGL  L MKP+  RIL+FAETR+EW I     FR+N P++TLYSTLGD+AIV+GI ++E  +VITS+ L+ ++                                         +G+  H M SVE +G  K     +S  S    P    LA+IMYTSGST  P+GVM+THGNLM  +A   + +P   R  DD YIGYLPLAHVLEL  E   L+ G+P+GYSSPLTL+D ++KIK  TKGDIS LKPT+MA VP I+DRI+K+VWEK  D G +   +F WAY+YK +RM RG PS  +DK++F K+K LLGG+VR MLSGGAPLS E Q F+N+CF  PI+QGYGLTET GAGT+    D    + G PL   QI++R+W EG YS  DQP PRGE+LIGG  VTLGYFK PDKTKEDFFVD DG+RWFCTGDIG+F+ +G+L I+DRKKDLVKLQGGEYISL KVE A+    FVE  CVY +S +D  +  + PK  Q++Q+AEK+ I           + + K K +  A+LK+LQ     K +ER E+P +V++   QWTP++GLVTDALKLKR+ +Q+ F  EI  MY+
Sbjct:    1 MSILFAV----VRAVTYVYDFVTWPLYYFLYAPDEVAK-NSR-----KVRAKRLSND-PESPWRCVESMDKLVEKPTLAVDSMADMLRIAVAKYPSKRCLGTRELLSEEDERQPNGRVFKKLCFGDYHWETYEEVNIRVDNFGRGLANLGMKPT-KRILVFAETRAEWQICVNAAFRFNHPVVTLYSTLGDDAIVYGINQAEVDIVITSDSLLGEI-----------------------------------------NGVQFHAMTSVERLG--KQQAAAKSTQSAASSPKSGDLAVIMYTSGSTGLPRGVMITHGNLMATIASAVDRIPQFGRRPDDTYIGYLPLAHVLELVAELACLTGGVPIGYSSPLTLSDVSNKIKKGTKGDISVLKPTLMAAVPAIMDRISKNVWEKVNDGGAVSKALFNWAYDYKKDRMLRGMPSPLLDKIIFNKVKSLLGGRVRVMLSGGAPLSTETQRFMNVCFGIPIVQGYGLTETTGAGTVQTVDDISTGRAGPPLACCQIRLRDWAEGGYSSRDQPCPRGEVLIGGNNVTLGYFKMPDKTKEDFFVDSDGIRWFCTGDIGLFNVNGTLEIVDRKKDLVKLQGGEYISLGKVETALGVSAFVEQLCVYGDSFKDHTVAIVVPKKTQVMQLAEKLGISGDTDATGDSYESLLKNKAIKDAVLKDLQSKAAGK-VERFELPTRVYLTSEQWTPESGLVTDALKLKRRQLQDHFQSEIDRMYS 685

[Close]

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. TrEMBL
Match: C4QPH5 (Long-chain-fatty-acid--CoA ligase OS=Schistosoma mansoni OX=6183 GN=Smp_103160.2 PE=4 SV=1)

HSP 1 Score: 712.22 bits (1837), Expect = 0.000e+0
Identity = 357/807 (44.24%), Postives = 499/807 (61.83%), Query Frame = 3

Query:  126 FQFK*RMSILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTL-----SSINSN---NNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKL--KMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFE----------GFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKP-TRDS-----------------------LAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRD-DKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKAN-TKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFCPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMN----------------------IEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYNR 2342
            F  K    + +PI +  +K+  Y YE+ TWP YY+F           S ++S+   N  + R LA P++  DP  PWR + + +GL +++ PGC  L D+ +R   LWP+  A GTR +++++  P P+ +   +LT  +Y WET+ D + R+++   GL  +   +K     I +F+ETR+EWM AAQ CFR N P+ TLY+TLGD+A+VHG  E+EA VV TS+EL+ K+ KI+++C S++ II+  N +F                 A   +       P  + LH++  VE +G+     D E   +    P T DS                       LA+IMYTSGST +PKGV MTH NL++A+AG  + LP +  + D YIGYLPLAHVLEL CE   +  G+ +GYS+P TLTD +S+IK +   GD+S L+PT+ A VPT+L+RI+K+VWEK    G     +F++AY YK  R+  G+PS  +D+L+F K++ L+GG++RY++ GGAPLSE++Q F NIC  PI+QGYGLTE+C  G LM  GD R N  G P   LQI++R W EG YSP D P PRGEIL+ G+ V+ GY+KQP+ T  DF  D+DG RWFCTGDIG+ H+DGSLSIIDRKKDLVKLQ GEY+SL+KVE+A+S+  +VE  CVY +  +++ ICF+SPK K L+ +AE +N                      IE  +   +CK  ++  A+LK+LQ+   EK L   EIP KV +D + WTPDTGLVTDALKLKR N+Q RF  EI+++Y +
Sbjct:   20 FTMKCLSKLFVPIGIEVIKIVVYAYEYLTWPLYYLFSKTLRYMPLPNSPVDSDWTYNEEESRTLALPVREGDPGSPWRAVEALDGLTSSL-PGCKDLVDVWLRTVKLWPDLPAFGTRAVLRTDYRPSPDGRQMIELTLGEYSWETFWDAERRVSHLAAGLYSILGSIKEHNQPIALFSETRAEWMFAAQACFRLNRPVATLYATLGDDALVHGFNETEAVVVFTSDELLSKVIKIVQRCPSIQRIIYFTNGVFNRELYTEVDNLAVPSGSATASVAEALTKCPSNLHLHDIIEVERLGAAVIMKDREKQTTSTSTPITGDSNHSSNGYEGLWMPPASERASPLDLAVIMYTSGSTGQPKGVEMTHENLVSAIAGHLQRLPKLRSNYDIYIGYLPLAHVLELTCELSCVIMGVRIGYSNPQTLTDTSSRIKRDYCLGDVSLLRPTLFASVPTVLERISKAVWEKVNAGGEFLQALFKFAYAYKCRRIHGGFPSFLVDRLIFRKVRSLMGGRIRYIVCGGAPLSEDSQLFTNICIAPIVQGYGLTESCSTGALMECGDLRCNHSGAPAPTLQIRLRAWPEGGYSPYDTPSPRGEILLSGKPVSRGYYKQPELTARDFITDQDGTRWFCTGDIGLMHTDGSLSIIDRKKDLVKLQAGEYVSLSKVEMALSQSAYVEQICVYVDPMQNYPICFVSPKLKSLIDLAESLNLVHLLHEARSKLPIESMKDSIAIEHVERAVLCKHPQIIQAVLKDLQRVAREKRLSTFEIPQKVALDTIAWTPDTGLVTDALKLKRFNLQTRFQHEIEELYQK 825

[Close]

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. TrEMBL
Match: A0A094ZIV3 (Long-chain-fatty-acid--CoA ligase 3 OS=Schistosoma haematobium OX=6185 GN=MS3_02079 PE=4 SV=1)

HSP 1 Score: 709.523 bits (1830), Expect = 0.000e+0
Identity = 357/799 (44.68%), Postives = 501/799 (62.70%), Query Frame = 3

Query:  150 ILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTL-----SSINSN---NNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKL--KMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVV----------PDGITLHEMGSVENMGSLKDYGDEE----------------SLDSY--LYKP------TRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRD-DKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKAN-TKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFCPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMN----------------------IEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYNR 2342
            +L+PI +  +K+  Y YE+ TWP YY+F           S ++S+   N  + R LA P++  DP  PWR + + +GL +++ PGC  L D+ +R   LWP+  A GTR +++++    P+ +   +LT  +Y WET+ D + R+++   GL  +   +K     I +F+ETR+EWM AAQ CFR N P+ TLY+TLGD+A+VHG  E+EA VV TS+EL+ K+ KI+++C S++ II+  N +F      +  +L    E+            P  + LH +  VE +G+     D E                S + Y  L+ P      +   LA+IMYTSGST +PKGV MTH NL++A+AG  + LP +  + D Y+GYLPLAHVLEL CE   +  G+ +GYS+P TLTD +S+IK +   GD+S L+PT+ A VPT+L+RI+K+VWEK    G     +F++AY YK  R+  G+PS  +D+LVF K++ L+GG++RY++ GGAPLSE++Q F NIC  PIIQGYGLTE+C  GTLM  GD R N  G P + LQI++R W EG YSP D P PRGEIL+ G+ V+ GY+KQP+ T  DF  D+DG RWFCTGDIG+ H+DGSLSIIDRKKDLVKLQ GEY+SL+KVE+A+ +  +VE  CVY +  +++ ICF+SPK K L  +AE +N                      IE  +   +CK  ++  A+LK+LQ+   EK L   EIP KV +D + WTPDTGLVTDALKLKR N+Q RF  EI+++Y +
Sbjct:    7 LLVPIGIEVIKIVVYAYEYLTWPLYYLFSKTLRYMPLPNSPVDSDWTYNEEESRTLALPVREGDPGSPWRAVEALDGLTSSL-PGCKDLVDVWLRTVKLWPDLPAFGTRAVLRTDYRLSPDGRQMIELTLGEYSWETFWDAERRVSHLAAGLYSMLGSVKEHNQPIALFSETRAEWMFAAQACFRLNRPVATLYATLGDDALVHGFNETEAVVVFTSDELLSKVIKIVQRCPSIQRIIYFTNGVFNRELYTEVDNLAVPSESATASVAEALTKCPSNLHLHNIVEVERLGAAVIMKDREKRKMGGSTPITGNNNHSSNGYEGLWMPPASERASPSDLAVIMYTSGSTGQPKGVEMTHENLVSAIAGHLQRLPKLRSNYDIYVGYLPLAHVLELTCELSCVIMGVRIGYSNPQTLTDTSSRIKRDHCLGDVSLLRPTLFASVPTVLERISKAVWEKVNAGGEFLQALFKFAYAYKCRRIHGGFPSFLVDRLVFRKVRSLMGGRIRYIVCGGAPLSEDSQLFTNICIAPIIQGYGLTESCSTGTLMECGDLRCNHSGAPASTLQIRLRAWPEGGYSPYDTPSPRGEILLSGKPVSRGYYKQPELTARDFITDQDGTRWFCTGDIGLMHTDGSLSIIDRKKDLVKLQAGEYVSLSKVEMALGQSVYVEQICVYVDPMQNYPICFVSPKLKSLTDLAESLNLVHLLHEARSKLPIESMKDPIAIEHVERAVLCKHPQIIQAVLKDLQRVAREKRLSTFEIPQKVALDTIAWTPDTGLVTDALKLKRFNLQTRFQHEIEELYQK 804

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Cavefish
Match: ACSL3 (long-chain-fatty-acid--CoA ligase 3 [Source:NCBI gene;Acc:103040373])

HSP 1 Score: 629.787 bits (1623), Expect = 0.000e+0
Identity = 332/729 (45.54%), Postives = 448/729 (61.45%), Query Frame = 3

Query:  159 PITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            PI +   +   +VY   T+  +YI      LS   +N     R+ A+ + +  P  P+R +NS   LV+ +  G +TLD +   A   +P +  LGTR+++  E E QPN K+FKK+    Y W +Y ++      FG GL  L  KP  + I +F ETR+EW+IAAQ CF YN P++TLYSTLG  AI HG+ E+E T +ITS++LM  +L +I+     ++HII +              D   T    +P GI +H M SV+++G+       E++     +P    +A+IMYTSGST  PKGVM++H N++  + G  E +P +   D YIGYLPLAHVLEL  E V +SHG  +GYSSP TL D+++KIK  +KGD S LKPT+MA VP I+DRI K+V  K  D       +F  AY YKM ++ RGY +   D LVF K++ LLGG  R +LSGGAPLS   Q F+NICFC P+ QGYGLTETCGAGT+    D    +VG PL   +I +++W+EG Y   D+P PRGEILIGG  VT+GY+K   K KEDFFVDK+G RWFCTGDIG F  DG L IIDRKKDLVKLQ GEY+SL KVE A+  CP ++  C YA S E +VI F+ P  KQL  +AE+  + +   ++IC   ++   +L  + +A +   LER EIP K+ +    WTP+TGLVTDA KLKRK ++  +  +I+ MY 
Sbjct:    9 PILLQVFRSVVWVYSVITFIPWYI------LSGAGANQARAKRIKARSV-SGHPSGPYRAVNSEQRLVSQLHEGVDTLDKVFEYAVKFFPERDCLGTREVLSEEDEVQPNGKVFKKVILGDYNWLSYEEVFQVAQRFGSGLAALGQKPQCN-IALFCETRAEWIIAAQACFMYNFPVVTLYSTLGGPAIAHGLNETEVTHIITSKDLMQSRLKEIVLDVPRLQHIILV--------------DRKPTSWPGLPRGIQVHSMASVQDLGA-----KPENMAVQRQRPLPHDIAVIMYTSGSTGIPKGVMISHSNIIAGVTGMAERIPGLDEKDTYIGYLPLAHVLELSAELVCVSHGCRIGYSSPQTLADQSTKIKKGSKGDTSVLKPTLMAAVPEIMDRIYKNVMVKVDDMSRFQKTLFVLAYNYKMEQISRGYGTPLCDNLVFRKVRSLLGGNTRVLLSGGAPLSAATQRFMNICFCCPVGQGYGLTETCGAGTISDMFDYSTGRVGAPLVCSEIMLKDWEEGGYYSTDKPNPRGEILIGGPNVTMGYYKNEAKNKEDFFVDKNGQRWFCTGDIGEFGPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAYANSDESYVISFVVPNQKQLQALAEQKGV-KGSWEEICNRPDMEKEVLHIITEAAVTAKLERFEIPKKIRLSAEAWTPETGLVTDAFKLKRKELKTHYQEDIERMYG 709

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Cavefish
Match: acsl4a (acyl-CoA synthetase long chain family member 4 [Source:NCBI gene;Acc:103036866])

HSP 1 Score: 602.823 bits (1553), Expect = 0.000e+0
Identity = 319/734 (43.46%), Postives = 448/734 (61.04%), Query Frame = 3

Query:  150 ILIPITVNALKLWTY-VYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYNR 2342
            +L+P+    L +W Y V  F  W F     SK  L+          R+ AK I+    + P+R+++ F  L     PG +TLD L   A   +  +  LGTR ++  E E QPN K+FKKL    Y W TY +++  + +FG GL  L  +P  + I IF ETR+EWMI AQ+CFR N P++T Y+TLG++A+  G+ E   T ++TS EL+  KL  ++ +  ++KHII+++N      G               P+ I +H M SV+ +G+       E+L+  + +P    LA+IMYTSGST +PKGV++ H NL+  M GQ E +P +   D YI YLPLAHVLEL  E   +++G  +GYSSP TL+D+++KIK  +KGD + LKPT+MA VP I+DRI K+V  K  +   +   +F  AY YK+ ++ +GY +   + ++F K++ LLGG VR MLSGGAPLS   Q F+NICFC P+ QGYGLTETCGAGT+    D    +VG PL   + ++R+W EG Y+  D+P PRGEILIGG  VT GY+K       DFFVD++G +WFCTGD+G  H+DG L I+DRKKDLVKLQ GEY+SL KVE A+  C  ++  C YA S +++VI F+ P  KQL  +A +  I     ++IC    +   +LKE++       L+R EIP KVH+    WTP+TGLVTDA KLKRK ++  ++ +I+ MY R
Sbjct:   11 LLLPV---HLFVWLYSVLSFLPWYFLTGASSKKILAK---------RIKAKSIKGQS-EGPYRSVDRFETLAYMDFPGKDTLDKLFDYAVERFGREHCLGTRAVLSEENETQPNGKVFKKLILGDYKWITYEEMEVEVNHFGSGLAALGQQPR-NTIAIFCETRAEWMITAQSCFRRNFPLVTFYATLGEDAVAFGLNECGVTHLVTSAELLETKLKSVLPQIPNLKHIIYVDNKSISTAG--------------YPETIQIHSMQSVKELGA-----KPENLNREIVRPIPSDLAVIMYTSGSTGRPKGVVIVHSNLIAGMTGQCERIPGLGPKDTYIAYLPLAHVLELTAEISCVTYGCRIGYSSPQTLSDQSTKIKKGSKGDCTVLKPTLMAAVPEIMDRINKNVMSKVQEMNCVQRTLFNLAYNYKLEQIKKGYDAPLCN-ILFKKVRALLGGNVRMMLSGGAPLSSATQRFMNICFCCPVGQGYGLTETCGAGTITEVADYSTGRVGAPLICCEFKLRDWAEGGYTKQDKPHPRGEILIGGPNVTKGYYKTDFSKNNDFFVDENGQQWFCTGDVGEVHTDGCLQIVDRKKDLVKLQAGEYVSLGKVESALKNCSLIDNICAYANSDQNYVISFVVPNQKQLTAMANQKGI-VGTWEEICNHPIMEMEVLKEIKNVAKTIKLQRFEIPVKVHLSPEPWTPETGLVTDAFKLKRKELKNHYLHDIERMYGR 709

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Cavefish
Match: acsl3a (acyl-CoA synthetase long chain family member 3a [Source:ZFIN;Acc:ZDB-GENE-050420-181])

HSP 1 Score: 554.673 bits (1428), Expect = 0.000e+0
Identity = 296/675 (43.85%), Postives = 406/675 (60.15%), Query Frame = 3

Query:  318 PQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            P  P+R++ S +GL   + PG NTLD +   +A  +  +  LGTR++++ E E Q + K+F+K+   +Y W ++  +    ++FG GL                     W+IAAQ CF  N P++TLYSTLG  AI HG+ ++E T +ITS+EL+    K+      ++HII ++       G               P GI++H M +V+ +G        E+       P    +A+IMYTSGST  PKGVM++H NL+ ++AG  E +P +  +D YI YLPLAHVLEL  E V +++G  +GYSSP TL D+++KIK  +KGD S L+PT+MA VP I+DRI K+V  K  +   L   +F  AY YKM ++ +G  +   DKLVF K++ LLGG+ R +LSGGAPLS   Q F+NIC C P+ QGYGLTETCGAGT+    D    +VG PL + +IQ+++W EG Y   D+P PRGEIL+GG  VT+GY+K     +E+FFVDK G RWFCTGDIG F +DG L IIDRKKDLVKLQ GEY+SL KVE  +   P ++  C YA S E +VI F+ P  KQL+ +AEK  I       IC    +   +LK +    L   LER EIP K+ +    WTP+TGLVTDA KLKRK ++  +  +I+ MY 
Sbjct:   38 PAGPYRDVTSLDGLATCLQPGLNTLDRVFETSAKRFSRQDFLGTREVLREEEELQESGKVFRKVVLGEYRWLSFEKVYRAASSFGSGL--------------------AWIIAAQACFMNNFPLVTLYSTLGGPAIAHGLNQTEVTHIITSKELLETKLKV-----QLQHIIVVDEKPTSCVG--------------YPRGISVHNMAAVQALGV-----KPENASRPRQLPAPSDIAVIMYTSGSTGVPKGVMISHSNLIASIAGMAERIPNLGSNDTYIAYLPLAHVLELSAEMVCVAYGCRIGYSSPQTLADQSTKIKTGSKGDTSVLQPTLMAAVPEIMDRIYKNVMMKVEEMSTLQKTLFILAYNYKMEQLAKGNSTPLCDKLVFKKVRALLGGRTRVLLSGGAPLSAATQRFMNICLCCPVGQGYGLTETCGAGTISQLWDYSTGRVGAPLVSCEIQLKDWAEGGYRNTDKPCPRGEILVGGPNVTMGYYKNEAMNRENFFVDKKGQRWFCTGDIGEFQADGCLKIIDRKKDLVKLQAGEYVSLGKVEAILKNNPLIDNICAYANSDESYVIGFVVPNQKQLMALAEKRGI-CGSWMDICNNSVIEEEVLKAITDTALSGQLERFEIPRKICLSAEAWTPETGLVTDAFKLKRKELKSFYQNDIERMYG 667

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Cavefish
Match: acsl4b (acyl-CoA synthetase long chain family member 4b [Source:ZFIN;Acc:ZDB-GENE-030131-6493])

HSP 1 Score: 539.265 bits (1388), Expect = 4.913e-180
Identity = 285/676 (42.16%), Postives = 413/676 (61.09%), Query Frame = 3

Query:  318 PQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFCPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKE-DFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            P+ P+R ++    LV     G +TLD L   A   +     LGTR ++  E E QP+ K+F+KL   +Y W++Y +++  +   G GL  L   P  S I I+ ETR+EW+I AQ CFR N P++TLY+TLG+EA+  G+ + +AT +ITS+EL+  KL  ++ +   +KH+I+       G G   A +         P  + +H M  V ++G+       E+L      P+   LA++MYTSGST +PKGVMM H NL+  MAGQ + +P +   D YIGYLPLAHVLEL  E   L++G  +GYSSP TLTD+++KI+  +KGD + LKPT++A VP I+DRI K+V  K  +   +   +FR A++YK+ +++ G  +   D LVF ++  LLGG VR MLSGGAPLS     FIN+CFCPI  GYGLTET GAGT+    D    +VG PL   +I++R+W EG Y+     +P          V +GY+    + +  +F+VD+ G RWFCTGD+G  H DG L I+DRKKDLVKLQ GEY+SLAK+E A      ++  CVYA+S++++VI F+ P  K+L ++A KM+I E + +++C    +   +LK +++      L+R E+P K  +    WTP+TGLVTDA KLKRK +Q  ++ +I+ MY 
Sbjct:   14 PEGPYRAVDCEEALVRQDFEGVDTLDKLFRHAVAKFKQTPCLGTRSIISKENETQPDGKVFQKLVLGEYSWKSYEEVEHEVELLGSGLAALGQTPK-SNIAIYCETRAEWIITAQACFRRNYPLVTLYATLGEEAVAFGLNQCKATHLITSKELLDTKLKVVLAEVPGLKHVIYA------GTGSVSAGNY--------PQSLAIHSMQEVLDLGA-----KPENLQVEYAPPSPSDLAVVMYTSGSTGQPKGVMMLHSNLIAGMAGQCQRIPGLG-SDVYIGYLPLAHVLELTAEISCLTYGCRIGYSSPQTLTDQSTKIQKGSKGDCTVLKPTLIAAVPEIMDRIYKNVMGKVCEMSMVQRTLFRMAFKYKLQQLENGANTPICDTLVFKRVCALLGGSVRLMLSGGAPLSALTHRFINVCFCPIAIGYGLTETSGAGTISEFSDFSTGRVGAPLICSEIKLRDWPEGGYT-----RPN---------VAVGYYGTAGEAESGEFWVDEAGQRWFCTGDVGEVHPDGCLQIVDRKKDLVKLQAGEYVSLAKIEAAAKNSSLIDNVCVYADSNQNYVISFVVPNQKRLTELARKMSI-EGEWEELCNNAVLEQEVLKAIKEIATTYKLQRFEVPLKARLSSQPWTPETGLVTDAFKLKRKELQRHYLHDIERMYG 653

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Cavefish
Match: acsl2 (long-chain-fatty-acid--CoA ligase 6 [Source:NCBI gene;Acc:103038184])

HSP 1 Score: 261.922 bits (668), Expect = 7.840e-75
Identity = 193/618 (31.23%), Postives = 300/618 (48.54%), Query Frame = 3

Query:  516 EKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRIL-IFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVE--NMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQ---FEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVG-YSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGY--PSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICF-CPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            E Y W  Y+++  R    G GLL    KP+  + + IFA+ R EW+IA   C+ +++ ++ LY TLG EA+VH +  +E ++VI  +E         EK  S    + +N    +  G   A   +  F  V    +   +   VE  ++  + D G E        KP    LA++ +TSG+T KPKG M+THGN+ +  +      EG   I ++D  I YLPLAH+ E   +  +  HG  VG Y   ++L             DI  LKPT    VP +L+RI   +    T   PL   +  +A   K   +  G    + F DKLV  KI+  LGG VR++L+  AP+S    +F+     C I +GYG TE     T  + GD     VG PL   ++++ +  + NY         GEI I G  V  GY    DK + D  +D DG  W  TGD+G +  +G+L IIDRKK++ KL  GEYI+  K+E   S+C  V    V+ +S + F+I  + P  +  +   +   I     +++C+  +V  A+L+++     + GL+  E    V++    ++   GL+T  LK +R +++  F  +I  MY+
Sbjct:  132 EPYQWIPYTEVAERAQVLGSGLLAKGCKPNPKQFVGIFAQNRPEWVIAELACYTFSMAVVPLYDTLGLEAMVHILNLAEISLVICDKE---------EKAES----LLLN----KEKGVTPALSCLVLFNPVSAALVERGKACGVEIMHLSEIMDLGRENLKTPVPPKP--QDLAVVCFTSGTTGKPKGAMITHGNIASNTSSVIKILEGFFVIRQEDVSISYLPLAHMFERMIQVSMFCHGARVGFYQGDISLL----------MDDIKTLKPTFFPVVPRLLNRIYDKIISSVTS--PLKRAILHYAVRRKQAELSCGIVRNNSFWDKLVLNKIQANLGGNVRFILTASAPISPTVLSFLRATLGCLIFEGYGQTECTAGCTFSMPGDWTAGHVGAPLPCARVKLTDIPDMNYY---ASSGEGEICIQGPSVFQGYLN--DKERTDEALDSDG--WLHTGDVGQWQPNGTLQIIDRKKNIFKLSQGEYIAPEKIENVYSRCVPVLQVFVHGDSLQSFLIGIVVPDPEVFVNWNKDRGI-VGSYEELCQNPDVKKAVLEDMTAVGKKAGLKSFEQVKDVYLHSELFSISNGLLTPTLKSRRVDLRRVFSEQINQMYS 710

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Sea Lamprey
Match: acsl3b (acyl-CoA synthetase long chain family member 3b [Source:ZFIN;Acc:ZDB-GENE-030131-492])

HSP 1 Score: 246.514 bits (628), Expect = 1.387e-74
Identity = 129/273 (47.25%), Postives = 175/273 (64.10%), Query Frame = 3

Query:  909 VPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICF-CPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEG 1724
            +P GI +  M  VE +G  K    + + D    +P    LA++MYTSGST  PKGV + H NL+ A+ G +  +  I  DD YIGYLPLAHVLEL  E V LS G  +GYSS  TLTD++++IK  ++GD++ L+PT+MA VP I++RI K V  K      L   +F+ AY YK+ +++RG+ +   ++LVF K+  LLGG VR +LSG APLS   Q F+N+CF CP+ QGYGLTETC AGT+    D     VG PL+  +IQ+R W+EG
Sbjct:   28 LPRGIEVMGMSDVEELGD-KPQHRQRARD----RPGPRDLAVVMYTSGSTGIPKGVRIAHSNLVAAITGMYLRINDICGDDTYIGYLPLAHVLELGAEMVCLSRGCRIGYSSAQTLTDQSTRIKKGSQGDVTILRPTLMAAVPEIMERIYKGVMGKVQCMSLLQRIIFKLAYNYKLEQLERGFDTPLCNRLVFNKLCALLGGNVRLLLSGAAPLSPRTQRFMNVCFCCPVGQGYGLTETCAAGTIAELQDYSTGHVGAPLSCCEIQLRNWEEG 295

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Sea Lamprey
Match: ACSL1 (acyl-CoA synthetase long chain family member 1 [Source:HGNC Symbol;Acc:HGNC:3569])

HSP 1 Score: 253.062 bits (645), Expect = 2.223e-72
Identity = 187/616 (30.36%), Postives = 302/616 (49.03%), Query Frame = 3

Query:  522 YYWETYSDIDTRITNFGRGLLKLKMKPSVSRIL-IFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSE-ELMPKLCKIIEK--CHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFE-----GLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVG-YSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFIN-ICFCPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            Y W +Y +   R    G G L L  KPS  +++ IF++ R EW+IA Q C+ Y++ ++ LY TLG E+I + I ++E ++V+  + E +  L + IE+     VK I+ M+   F+G    +A+            GI L     +E +G        E +      P  D +A+I YTSG+T  PKG M+TH N+++  +          LP  + DD  I +LPLAH+ E   E  +L +G  VG Y   + L             D+  L+PT+   VP +L+RI   V   A  +   +L  F  + +    R          DKL+F +++  +GG+VR+M++G AP+S     F+  I  C + +G+G TE     T  +  D     VG P+    I++ +  E NY   +     GE+ + G  V  GY K P +T E   +D+DG  W  TGDIG +  +G+L I+DRKK + KL  GEYI+  K+E    +   V    V+ +S +  ++C + P  + L    +K  I + +   +   K+V +A+L ++ +   E GL+  E    VH+    ++ + GL+T   K+KR  + + F  EI  +Y
Sbjct:  120 YEWISYKETSDRAEYLGSGFLHLGAKPSSEQVIGIFSQNRPEWIIAEQACYTYSLVVVPLYDTLGRESIDYIINQAEISMVVCDKLEKVKGLLESIEEGAIRIVKTIVVMDP--FDGVMEQRARKC----------GIDLILFRELEVIGKTNH---REPI-----PPQPDDIAIICYTSGTTGNPKGAMLTHKNMISDFSAFLAITKDTFLP--NTDDVLISFLPLAHMFERLVEASILCNGGRVGFYQGDIRLL----------MDDMKVLQPTVFPVVPRLLNRIHDKVLSGAKSHFKRWLLEFAVSRKIAELRCGVVRKDSIWDKLIFHRVQASMGGRVRFMVTGAAPISASILTFLRAILGCQVYEGFGQTECTAGCTFTMPSDSTAGHVGPPMPCNHIKVVDVAEMNYFAANG---EGEVCVYGTNVFKGYLKDPTRTAEA--LDEDG--WLHTGDIGKWLPNGTLKIVDRKKHIFKLSQGEYIAPEKIETVYVRSEPVAQVFVHGDSLQSCLVCIVVPDMEVLPSWVQKRGI-KCNPNSVFINKDVRAAILHDMVRLGKEAGLKSFEQVRAVHLHSDLFSIENGLLTPTFKVKRAEVCKFFHSEIDSLY 695

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Sea Lamprey
Match: ACSL1 (acyl-CoA synthetase long chain family member 1 [Source:HGNC Symbol;Acc:HGNC:3569])

HSP 1 Score: 217.624 bits (553), Expect = 3.374e-62
Identity = 150/468 (32.05%), Postives = 233/468 (49.79%), Query Frame = 3

Query:  960 SLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQF---EGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGY--SSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGY--PSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICF-CPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            SL+D G      +   KP  + LA++ +TSG+T  PKG M+TH N+++ M+G     E L      D  I YLPLAH+ E   +  +  HG  +G+       L D           D+ AL+PT+   VP IL+R+   V    +   P   ++  +    KM  + RG    +   D LVF  ++  +GG+VR +++G AP+S    NF+ +   C + +GYG TE     TL + GD +   VG P+    I++ + K+  Y      + +GE+ + G  V  GY K  +KT E   VD+DG  W  TGDIG +  +G+L IIDRKK + KL  GEYI+  K+E   S+C  V    V+ +S +  ++  + P  + L     K  I      ++C+ KEV  A+L+++Q+   E  L+  E    VH+    ++ + GL+T   K KR  ++  F   I  MY 
Sbjct:    1 SLQDCGRMHKRTTLPPKP--EDLAIVCFTSGTTGNPKGAMLTHRNIVSDMSGFLKVTESLFLPETSDIAISYLPLAHMFERLVQATLFCHGASIGFFQGDVRLLLD-----------DMQALRPTVFPVVPRILNRMYDKVL--GSTRTPFRRKLLEFGARRKMAELQRGVVRRNSLWDWLVFRPMQLSVGGRVRMIMTGAAPISPGVLNFLRVVMGCQMYEGYGQTECTAGCTLTLPGDWKAGHVGAPMPCNYIKLHDVKDMEYY---TSQGKGEVCVKGPNVFKGYLKDAEKTAEA--VDRDG--WLHTGDIGQWMPNGTLKIIDRKKHIFKLAQGEYIAPEKIENVYSRCEPVAQVYVHGDSLQACLVAVVVPDPEILCCWIRKKGI-VGTYSELCRNKEVRQAILEDMQRLGKESDLQPFEQVKDVHLHNEMFSIENGLLTPTFKAKRTELRTHFRSVISSMYQ 445

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Sea Lamprey
Match: acsbg2 (acyl-CoA synthetase bubblegum family member 2 [Source:ZFIN;Acc:ZDB-GENE-030131-7099])

HSP 1 Score: 102.449 bits (254), Expect = 1.679e-22
Identity = 125/565 (22.12%), Postives = 233/565 (41.24%), Query Frame = 3

Query:  387 TLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNL--MTAMAGQFEGLPPIHRDDKY----IGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWA----YEYKMNRMDRGYPS------VFMDKLVFAKIKKLLG-GKVRYMLSGGAPLSEEAQNFINICFCPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAI-SKCPFVETACVYAE 2027
            T++ L  R   L+ +  AL +++              +K LT+++YY         +     +  LKL ++     + I     +EW I+           + +Y+T   EA  +      A +++   +   +L KI+E    + H+  +  Y  E              +   P+  +  E      M + ++  D + LD+ +     +    ++YTSG+T  PKGVM++H NL     + G+   L     DD Y    I YLPL+H+        ++   +P+ +   +T   +   +K +    +  ++PT    VP + +++ + +   A  +G    ++  WA     +  +  M +G P+         + LVF K+++ LG  +     +G AP++ +   F      P+ + YG++E+ G  T+      ++   G  L   +  +    E         +  GE+   G  V +GY    +KT+E   +D DG  W  +GD+G     G L I  R K+L+   GGE +    +E AI  + P V  A V  +
Sbjct:   82 TINQLFERTVKLYGDHKALASKE-----------GNTWKILTYKEYY--------AQCRQAAKSFLKLGLQ-RYHGVGILGFNSAEWFISDIGAILAGGFAVGIYTTNSAEACQYVADNCLANIIVVEND--KQLQKILEVQSQLPHLKAIVQYKGE-------------LKEKKPNVYSWAEF-----MRAGEEVPDAQ-LDAIIASQRANQCCSLIYTSGTTGSPKGVMLSHDNLTWTATVTGELTQLR--SADDNYQEVVISYLPLSHIAAQ-----LVDIWLPMRFGG-VTYFAQPDALKGSLVVTMKEVRPTGFMGVPRVWEKMMEKMKATAAKSGIFKRKIAGWAKSVGLQTHLANM-KGNPAEAPMGYTLANMLVFKKVREALGLDRCTKCYTGAAPITRDTLEFFLSLDIPVFELYGMSESSGPHTISSPTAFQIGSCGTLLRGCRTLLHSKDE---------EGSGEVCFWGRHVFMGYLNMEEKTRE--AIDDDG--WLHSGDLGRVDEQGLLYITGRIKELIITAGGENVPPIPIEDAIKEELPVVSNAMVIGD 583

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005646.1 (pep scaffold:Pmarinus_7.0:GL484188:1842:9754:1 gene:ENSPMAG00000005099.1 transcript:ENSPMAT00000005646.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 62.003 bits (149), Expect = 9.300e-11
Identity = 31/99 (31.31%), Postives = 52/99 (52.53%), Query Frame = 3

Query:  522 YYWETYSDIDTRITNFGRGLLKLKMKPSVSRIL-IFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCH 815
            Y W +Y ++  R   FG GL+    +P+  + + +FA+ R EW+I  Q C+ Y++ ++ LY TLG EAI   II   A + +        +C  +E+  
Sbjct:  118 YQWMSYKEVAERSEWFGSGLIHKGCRPATDQFIGVFAQNRPEWIITEQACYMYSMVVVPLYDTLGQEAI-RFIINRAAEIAVV-------VCDTVERAR 208

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Yeast
Match: FAA1 (Long chain fatty acyl-CoA synthetase; activates fatty acids with a preference for C12:0-C16:0 chain lengths; role in the competitive import of long-chain fatty acids and sphingoid long-chain bases; accounts for most acyl-CoA synthetase activity; localizes to lipid particles and the plasma membrane; role in sphingolipid-to-glycerolipid metabolism; forms ER foci upon replication stress; faa1 faa4 double null complemented by any of human ACSBG1, ACSL1, 3, 4, 5, 6, SLC27A2, or 4 [Source:SGD;Acc:S000005844])

HSP 1 Score: 316.62 bits (810), Expect = 1.278e-95
Identity = 211/665 (31.73%), Postives = 345/665 (51.88%), Query Frame = 3

Query:  381 CNTLDDLMIRAANLWPNKSALGTR--KLVKSEMEPQPNKKLFKKLTFEK---------YYWETYSDIDTRITNFGRGLLKLKMKPSVS-RILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFE-----GFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAG-QFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRW-AYEYKMNRMDRGYPSV-FMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFCPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNI-EEKD----IQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNI 2300
            C+T+ + ++       N +A+G R  K +  E +    K   K+ + EK         Y++ ++  +   +   GRGL+K+ +KP+   ++ ++A T  +WM          IP++T Y TLG++ ++H ++++ +  + T   L+P L K ++    VK+II  ++   E     G     A D I   + V PD  T     S +++  L   G E   +  ++ P +D L  IMYTSGST +PKGV++ H N++  + G     L  +   D+ I +LPLAH+ EL  E +    G  +GY++  TLT  + +   N +GD+   KPTIM  V  + + + K +  +  DN P   +   W AY  K+N      P    +  LVF KI+   GG++RY+L+GG+P+S +AQ FI    CP++ GYGLTETC + T++   +  +   G     + +++ + +E  Y   +    +GE+ I G  VT  Y+K  ++T +    D     WF TGDIG + ++G L IIDRKK+LVK   GEYI+L K+E       +V   CVYA+ S+   +  I P H  L ++A+K+ I E+KD    I+   +  ++  A+  +L K   ++GL  +E+   +     +WTP  G VT A KLKRK+I
Sbjct:   39 CSTVYEFVLECFQKNKNSNAMGWRDVKEIHEESKSVMKKVDGKETSVEKKWMYYELSHYHYNSFDQLTDIMHEIGRGLVKIGLKPNDDDKLHLYAATSHKWMKMFLGAQSQGIPVVTAYDTLGEKGLIHSLVQTGSKAIFTDNSLLPSLIKPVQAAQDVKYIIHFDSISSEDRRQSGKIYQSAHDAINRIKEVRPDIKTF----SFDDILKL---GKESCNEIDVHPPGKDDLCCIMYTSGSTGEPKGVVLKHSNVVAGVGGASLNVLKFVGNTDRVICFLPLAHIFELVFELLSFYWGACIGYATVKTLTSSSVR---NCQGDLQEFKPTIMVGVAAVWETVRKGILNQ-IDNLPFLTKKIFWTAYNTKLNMQRLHIPGGGALGNLVFKKIRTATGGQLRYLLNGGSPISRDAQEFITNLICPMLIGYGLTETCASTTILDPANFELGVAGDLTGCVTVKLVDVEELGYFAKNN---QGEVWITGANVTPEYYKNEEETSQALTSDG----WFKTGDIGEWEANGHLKIIDRKKNLVKTMNGEYIALEKLESVYRSNEYVANICVYADQSKTKPVGIIVPNHAPLTKLAKKLGIMEQKDSSINIENYLEDAKLIKAVYSDLLKTGKDQGLVGIELLAGIVFFDGEWTPQNGFVTSAQKLKRKDI 685

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Yeast
Match: FAA3 (Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C16:0-C18:0 chain lengths; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery [Source:SGD;Acc:S000001271])

HSP 1 Score: 300.056 bits (767), Expect = 1.309e-89
Identity = 201/619 (32.47%), Postives = 321/619 (51.86%), Query Frame = 3

Query:  522 YYWETYSDIDTRITNFGRGLLKLKMKPS-VSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFM-----NNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPP-IHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSV-FMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFCPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKE-GNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEK--DIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYNRH 2345
            Y   TY ++   + + GRGL K+ +KP+   +  IFA T  +WM     C    IP++T Y TLG+  ++H ++E+E+  + T  +L+ K+   ++    +K +I       N+    G     A+D I     V PD I ++    V  +G  K   DE  L    + P    LA IMYTSGS   PKGV++TH N+++ +AG    +   I   D+ + +LPLAH+ EL  E         +GY S  TLT+ +++   N KGD+   KPTIM  V  + + + K++ EK +D  P+  ++F  AY  K    ++  P   F+ ++VF K+++  GG ++Y+++GG+ +S +AQ F +I  CP+I GYGLTET     ++         VG  + ++  ++ + K+ G Y+  +Q    GE+L+ G  V   Y+K P +T   F  D     WF TGDI  +   G L IIDR+K+LVK   GEYI+L K+E       +V+  CVYA+ S    +  + P    L + A K+ I +K  DI+     K + +A+ KE+      +GL  +E+   +     +WTP+ G VT A KLKR+ I      E++ +Y  +
Sbjct:   97 YKMMTYQELIWVMHDMGRGLAKIGIKPNGEHKFHIFASTSHKWMKIFLGCISQGIPVVTAYDTLGESGLIHSMVETESAAIFTDNQLLAKMIVPLQSAKDIKFLIHNEPIDPNDRRQNGKLYKAAKDAINKIREVRPD-IKIYSFEEVVKIG--KKSKDEVKL----HPPEPKDLACIMYTSGSISAPKGVVLTHYNIVSGIAGVGHNVFGWIGSTDRVLSFLPLAHIFELVFEFEAFYWNGILGYGSVKTLTNTSTR---NCKGDLVEFKPTIMIGVAAVWETVRKAILEKISDLTPVLQKIFWSAYSMK----EKSVPCTGFLSRMVFKKVRQATGGHLKYIMNGGSAISIDAQKFFSIVLCPMIIGYGLTETVANACVLEPDHFEYGIVGDLVGSVTAKLVDVKDLGYYAKNNQ----GELLLKGAPVCSEYYKNPIETAVSFTYDG----WFRTGDIVEWTPKGQLKIIDRRKNLVKTLNGEYIALEKLESVYRSNSYVKNICVYADESRVKPVGIVVPNPGPLSKFAVKLRIMKKGEDIENYIHDKALRNAVFKEMIATAKSQGLVGIELLCGIVFFDEEWTPENGFVTSAQKLKRREILAAVKSEVERVYKEN 693

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Yeast
Match: FAA4 (Long chain fatty acyl-CoA synthetase; activates fatty acids with a preference for C12:0-C16:0 chain lengths; role in the competitive import of long-chain fatty acids and sphingoid long-chain bases; role in stationary phase survival; localizes to lipid particles and the plasma membrane; role in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon replication stress; faa1 faa4 double null complemented by any of human ACSBG1, ACSL1, 3, 4, 5, 6, SLC27A2, or 4 [Source:SGD;Acc:S000004860])

HSP 1 Score: 270.781 bits (691), Expect = 4.714e-79
Identity = 188/646 (29.10%), Postives = 326/646 (50.46%), Query Frame = 3

Query:  453 KLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPS-VSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIF----------MNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPP-IHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYP-SVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFCPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNI--EEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYNRH 2345
            K V  + +P     L+ +LT   Y   TY ++   + + GRGL+K+ +KP+  ++  IFA T  +WM     C    IP++T Y TLG+  ++H ++E+++  + T  +L+ KL   ++   +VK +I            N  +++      A+D +   + V PD I ++    +  +G  K   DE  L    + P  +  A IMYTSGST  PKGV++TH N++  + G    +   I   D+ I +LPLAH+ EL  E         +GY++  TLT  +++   N +GD+   KPT+M  V  + + + K +  K  +       +F   Y  K    +R  P S  +  L+F +I++  GG +R++L+GG+ +S +AQ F++   CP++ GYGLTE      ++          G  +  +  ++ + ++  Y   +    +GE+L  G  +   Y+K P++T   F  D     WF TGDI  +   G + IIDRKK+LVK   GEYI+L K+E      P+V+  CVYA+ ++   +  + P    L ++A ++ I    +D++     K++  A+ K++      +GL  +E+   +     +WTP+ GLVT A KLKR++I      +++ +Y  +
Sbjct:   76 KRVDGKDKPIEKTWLYYELT--PYITMTYEEMICVMHDIGRGLIKIGVKPNGENKFHIFASTSHKWMKTFLGCMSQGIPVVTAYDTLGESGLIHSMVETDSVAIFTDNQLLSKLAVPLKTAKNVKFVIHNEPIDPSDKRQNGKLYKA-----AKDAVDKIKEVRPD-IKIYSFDEIIEIG--KKAKDEVEL----HFPKPEDPACIMYTSGSTGTPKGVVLTHYNIVAGIGGVGHNVIGWIGPTDRIIAFLPLAHIFELTFEFEAFYWNGILGYANVKTLTPTSTR---NCQGDLMEFKPTVMVGVAAVWETVRKGILAKINELPGWSQTLFWTVYALK----ERNIPCSGLLSGLIFKRIREATGGNLRFILNGGSAISIDAQKFLSNLLCPMLIGYGLTEGVANACVLEPEHFDYGIAGDLVGTITAKLVDVEDLGYFAKNN---QGELLFKGAPICSEYYKNPEETAAAFTDDG----WFRTGDIAEWTPKGQVKIIDRKKNLVKTLNGEYIALEKLESIYRSNPYVQNICVYADENKVKPVGIVVPNLGHLSKLAIELGIMVPGEDVESYIHEKKLQDAVCKDMLSTAKSQGLNGIELLCGIVFFEEEWTPENGLVTSAQKLKRRDILAAVKPDVERVYKEN 693

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Yeast
Match: FAA2 (Medium chain fatty acyl-CoA synthetase; activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome; comparative analysis suggests that a mitochondrially targeted form may result from translation starting at a non-canonical codon upstream of the annotated start codon [Source:SGD;Acc:S000000817])

HSP 1 Score: 189.119 bits (479), Expect = 2.165e-50
Identity = 183/690 (26.52%), Postives = 303/690 (43.91%), Query Frame = 3

Query:  357 LVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKL----KMKPSVSR---ILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEG--LPPIHRDDKY--IGYLPLAHVLELCCENVVLSHGIPVGY---SSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMD-RGYPS-VFMDKLVFA-----KIKKLLG-GKVRYMLSGGAPLSEEAQNFINICF-CPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVE-LAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEK------GLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            LV+ +     T  D  + +A  WP +  LG+R + K+    +           E + +E+YS +  R  N G G+L L    + +P  +    + I +    EW++    C  Y++    LY TLG     + +  +EA ++I ++  M  + K++     V  ++ M+    +       + L+    N + + IT   +  VE +G                 PT DSL  I +TSG+T  PKGV M+H N+ + +A  F    +PP  R+ +   + +LPLAH+ E       L+ G  +G+     P  L +           D+  LKP  +A VP IL R    + + A D   +   +     + K  R   RG P    M+ LV+      KI+  LG     ++++G AP+S++   F+       I QGYGLTET     L    ++ V   G    + + +++   E  Y      K  GE+ I G  V   YFK P++T +   VD+DG  WF TGD+      G +S+IDR K+  KL  GEYI+  K+E + +S CP++    V+ +  + F++  +         I    + E K   K   ++ +N    K+L+K  L K      GL+  E  H + +     T +  +VT   K+KR    + F   +  +Y
Sbjct:   84 LVSCVDKNLRTAYDHFMFSARRWPQRDCLGSRPIDKATGTWE-----------ETFRFESYSTVSKRCHNIGSGILSLVNTKRKRPLEANDFVVAILSHNNPEWILTDLACQAYSLTNTALYETLGPNTSEYILNLTEAPILIFAKSNMYHVLKMVPDMKFVNTLVCMDELTHDELRMLN-ESLLPVKCNSLNEKITFFSLEQVEQVGCFNKIP--------AIPPTPDSLYTISFTSGTTGLPKGVEMSHRNIASGIAFAFSTFRIPPDKRNQQLYDMCFLPLAHIFERMVIAYDLAIGFGIGFLHKPDPTVLVE-----------DLKILKPYAVALVPRILTRFEAGI-KNALDKSTVQRNVANTILDSKSARFTARGGPDKSIMNFLVYHRVLIDKIRDSLGLSNNSFIITGSAPISKDTLLFLRSALDIGIRQGYGLTETFAGVCLSEPFEKDVGSCGAIGISAECRLKSVPEMGYHADKDLK--GELQIRGPQVFERYFKNPNETSKA--VDQDG--WFSTGDVAFIDGKGRISVIDRVKNFFKLAHGEYIAPEKIENIYLSSCPYITQIFVFGDPLKTFLVGIVGVDVDAAQPILAAKHPEVKTWTKEVLVENLNRN--KKLRKEFLNKINKCTDGLQGFEKLHNIKVGLEPLTLEDDVVTPTFKIKRAKASKFFKDTLDQLY 733

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Yeast
Match: PCS60 (Oxalyl-CoA synthetase; capable of catalyzing conversion of oxalate to oxalyl-CoA; catalyzes first step in pathway of oxalate degradation that functions to protect yeast from inhibitory effects of oxalate; peroxisomal protein that binds mRNA; localizes to both peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid; similar to E. coli long chain acyl-CoA synthetase [Source:SGD;Acc:S000000426])

HSP 1 Score: 63.1586 bits (152), Expect = 1.324e-10
Identity = 112/443 (25.28%), Postives = 182/443 (41.08%), Query Frame = 3

Query: 1026 LAMIMYTSGSTDKPKGVMMTHGNLMTA---MAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHG-------IPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICF-CPIIQGYGLTETCGAGTL--MVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVH-IDYLQWTPDTGLVTDALKLKRKNIQERF 2312
            +A+I++TSG+T  PK V + H N++ +   +A  ++ L P+ R   Y+  +PL HV  L    V+LS         +P G+  P    D+  K   N             +CVPTI                              M  ++   P+ F                +R++ S  + L+    + +   F  P+++ Y +TE     T   +  G R+   VG P     + + +    N  P   P   GE+ I GE VTLGY   P   KE+F   ++   +F TGD G F  +G L +  R K+L+  +GGE IS  +++  +   P ++ A  +    +          + Q++Q A    I  K  +K+   + VN           L+K L   +IP KV+ +D L  T  TG      K++R+ I E F
Sbjct:  191 VALILHTSGTTSTPKTVPLLHLNIVRSTLNIANTYK-LTPLDRS--YV-VMPLFHVHGLI--GVLLSTFRTQGSVVVPDGFH-PKLFWDQFVKYNCN-----------WFSCVPTI-----------------------------SMIMLNMPKPNPF--------------PHIRFIRSCSSALAPATFHKLEKEFNAPVLEAYAMTEASHQMTSNNLPPGKRKPGTVGQPQGVTVVILDD--NDNVLP---PGKVGEVSIRGENVTLGYANNPKANKENFTKREN---YFRTGDQGYFDPEGFLVLTGRIKELIN-RGGEKISPIELDGIMLSHPKIDEAVAFGVPDD---------MYGQVVQAA----IVLKKGEKMTYEELVN----------FLKKHLASFKIPTKVYFVDKLPKT-ATG------KIQRRVIAETF 533

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Nematostella
Match: EDO29879 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T2D4])

HSP 1 Score: 647.121 bits (1668), Expect = 0.000e+0
Identity = 317/677 (46.82%), Postives = 452/677 (66.77%), Query Frame = 3

Query:  318 PQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGL-PPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMF-RWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            P  P+R++  F+ LV T+  G  T+D L  R+  L+  +  LGTR+ +  E E QPN ++FKK    +Y W TY+++D+R  N G GL+ L  +P    I++FAET+++WM++A +CF +  P++T+Y+TLGDEA+ HGI E+E   VIT   L+PKL  +  +   ++HII+M         GN  + ++  F    P  I LH M  VE +GS +  G+ +S     Y P  +  A+IMYTSGST  PKGV+++H NL+ A A     + P I   D Y+GYLPLAHVLEL  EN +L+HG  +GYSSPLTL+D++SKIK  +KGD++ L+PT+MA VP I+DRI ++V EK  D GP  L++F  +AY YK+ ++ RGY +  +++ +FAK++KLLGG+VR MLSGGAPLS + Q F+N+CFC P+ QGYGLTETCG GT+    DR   +VG P+ + ++++  W+EG Y+  D+P  RGEI+IGG  VTLGY+K P KT+EDF VD++G RWF TGDIG FH DG L IIDRKKDLVKLQ GEY+SL KVE A+++C +VE  CV+A+S + F +C + P+ KQL Q+A+ + +   D   +C  K +  A+LK+++       LE+ EIP ++ +   QWTP++ LVT ALKLKRKNI   +   ++ +YN
Sbjct:   53 PGAPYRDVEHFDALVTTLYKGITTMDQLFRRSVQLYAFRKCLGTRETLSIEEETQPNGRVFKKEIRGEYNWLTYAEVDSRARNLGNGLITLGQQPR-QNIVVFAETQADWMVSALSCFGFGFPVVTIYATLGDEAVAHGINETEVNFVITDSVLLPKLAAVSGQLTQLQHIIYM---------GNAKKSVLMNF----PSSIKLHSMVEVEELGSRQ--GNAQSR----YVPHPEDTAVIMYTSGSTGLPKGVIISHANLLAATAAMGARVEPSITEKDVYVGYLPLAHVLELVAENALLAHGACIGYSSPLTLSDQSSKIKKGSKGDLTVLRPTLMAAVPVIMDRIRQNVMEKVKD-GPRLLQLFFTFAYNYKLQQIKRGYDAPLLNRFIFAKLRKLLGGRVRQMLSGGAPLSSDTQYFMNVCFCCPVGQGYGLTETCGGGTICHTWDRTTGRVGPPIASCEVKLVSWEEGGYTIRDKPFARGEIVIGGSNVTLGYYKNPQKTQEDFEVDRNGQRWFHTGDIGEFHDDGCLKIIDRKKDLVKLQSGEYVSLGKVEAALAQCQYVENVCVFADSLKTFCVCLVVPRPKQLKQLADSLQVPTDDWSAVCSNKAIREAVLKDIRIVGNSAKLEKFEIPQRMSLVLEQWTPESELVTAALKLKRKNITSFYQDTLRTLYN 708

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Nematostella
Match: EDO45461 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RSR3])

HSP 1 Score: 240.736 bits (613), Expect = 7.063e-68
Identity = 180/613 (29.36%), Postives = 296/613 (48.29%), Query Frame = 3

Query:  522 YYWETYSDIDTRITNFGRGLLKLKMKPSVSRIL-IFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGY--SSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGY--PSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICF--CPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            Y W +Y+++  R  +FG  L+ L   PS    + I A+   EW++    C  +++  + +Y T G +A +  I  ++   ++ +   +P + K I++   +K II +   + E     + +D   +       GI L      E +G              +  P  + +  I YTSG+T  PKG + TH N++  ++G           D +  YLPLAH  E      +L  G  +G+    P  L +           DI  LKPT  A VP I++++   V  + +++      MF+     K   M++     +   DK+VF K++ L GG+VR + SG APLS +   F       C I++GYG TE     +L +  D +   VG PL    I++ +  E +YS  D    +GE+ I G  V   Y K P+KT E   +D+DG  W  TGDIG++  +G+L IIDRKKD+ KL  GEY++  K+E    + PFV  A V+ ES + FV+  + P  + L + A + NI + D++ +C+ +EV  A+ +++ + C E  L   E    VH+    ++ +  L+T   K+KR  I + F   I+ +Y+
Sbjct:   90 YRWLSYNEVRERAHSFGSALVHLGCHPSQRTFVGIQAKNCVEWVLTDLACQMFSMVSVPIYDTHGPDACLFIINHADVETIVCNHTKVPFILKNIKRIPHLKCIIQIGELVKE----EERKDAKVS-------GIELLSFSIAEEIGRKNI--------KVVNPPKPNDIFTICYTSGTTGTPKGAVQTHSNVIADVSGVIRQYNTCG-IDVHFSYLPLAHAYERTGVICMLQSGCRIGFYRGDPQLLFE-----------DIQELKPTFFASVPRIMNKVYDKVMAQVSES-KFKSWMFQKGLHAKKADMNKNVFRSNTIWDKVVFKKVQMLFGGRVRAITSGAAPLSPKVMTFFRCAMGDCRIMEGYGQTECTACASLQLPQDMQAGIVGPPLTCNYIKLVDVPEKSYSTKDL---KGEVCIKGPNVIPRYHKNPEKTAET--LDEDG--WLHTGDIGMWLENGALKIIDRKKDIFKLAQGEYVAPGKLENIAQRSPFVAQAFVHGESLKTFVVAILVPDGEVLKKWAAQKNI-QGDLKTLCEKEEVRQAVFEDVVRVCKEAELFSFEQVKAVHLHPEAFSVENDLMTATFKIKRPQIIKAFHDVIESLYH 662

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Nematostella
Match: EDO46335 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQC0])

HSP 1 Score: 236.884 bits (603), Expect = 8.823e-67
Identity = 189/671 (28.17%), Postives = 308/671 (45.90%), Query Frame = 3

Query:  357 LVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMK--PSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIF--EGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPP----IHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGY--PSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFCPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            L   M   C T  ++ +R A +  +K  LG R    +E  P              Y W TY+++  R +N G GL +L        + I ++A  + EW++  Q C  +   I  LY TLG +A    I +++ ++V+     +P L +  ++C ++K II +N  +   E   G +             +GI +     VE MG    Y  E+ L      P  D LA++ YTSG+T  PKG M+TH N +  +AG    +      +  DD +I YLPLAH+ E   + ++  +G  VG+           KI      D+  LKPT+   VP +L+R+   V  K   +  +   +F  A  +K   ++R     +   D LVF KI+                               +++GYG TET  A T+ + GD     VG P+   +I++ +  + NY   D    +GEI   G  V  GY   P+KT+E   +D+DG  W  +GD+G +  +G+L +IDRKK + KL  GEYI+  K+E    +CP V    VY +S +  ++  + P+   L +  EK  + + D++ +C  ++V  A+L+E+     ++ L   +    +++    +T D GL+T  LK KR  IQ  F    + +Y+
Sbjct:   68 LTAYMFEDCRTTHEVFLRGARISGDKPCLGHRV---TEGGP--------------YEWLTYNEVLNRASNVGSGLQELGSNDVEGDTFIGVYASNKIEWVLIEQACAMHAKVITPLYDTLGPDACAFIINQAKMSMVVCDAAKIPLLLQRADQCPTLKFIIKINPDVTSEEKQEGER-------------NGIKIISFSDVEAMGKAHPY--EKKL------PKPDDLAVVCYTSGTTGNPKGAMITHSNAVAMLAGLCVMIKKCGIRVDNDDVHISYLPLAHMYERLAQLMIFMYGGKVGF-----FGGNIRKIME----DLQELKPTVFISVPRVLNRVYDKVM-KEISSSSIKKFVFNTALSFKKAEVERNIVRKNSIWDYLVFNKIQ-------------------------------VLEGYGQTETTAAATIQLVGDHTFGHVGPPMPCTKIKLVDVPDMNYFAKDG---KGEICFYGPNVFKGYLHDPEKTREA--IDEDG--WLHSGDVGEWTENGTLKLIDRKKHIFKLAQGEYIAPEKIEAVYMQCPLVHQVFVYGDSRKSCIVGVVVPEETALKKWTEKNEV-QGDMEALCNNEKVRKAILEEMSAMGKKEKLFSFQQVKDIYLHPDPFTVDNGLLTPTLKFKRPVIQAHFKDTFEKLYD 651

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Nematostella
Match: EDO45504 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RSR2])

HSP 1 Score: 224.557 bits (571), Expect = 1.032e-62
Identity = 185/666 (27.78%), Postives = 311/666 (46.70%), Query Frame = 3

Query:  384 NTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRIL-IFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCH---SVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTR-DSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQF-------EGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVG--YSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFIN-ICFCPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            NT+ +  +R   +  N   LGTR+  +                   Y W TYS I  R +NFG GL +L   PS    + +++   +EW++A   C  +++  + +Y + G EA  + I +++ + ++  ++   K+  ++E  H   ++K II ++     G       D++         GI +     VE +G           + +   P R +    I +TSG+T  PKGV++TH N+++ M+          E L P    D ++ YLP  H+ E C + +++  G  +G  +     L D           D  AL+PT+ A VP +L+ I   V         LF     + +  ++ R D        D+LVF KI+ L+GG VR +  G APLS    +FI  +  C + +GYG TET    T+    D     VG P+    +++ +  E +Y   +    +GEI I G  V  GY    ++T+E F    D   WF +GDIG +  DG+L IIDRKK + KL  GEYI+ AK++ A  +  FV    V+  S + F++  + P  + L+  A+  +I E DI+++C+ + V  A+  ++     E  L  +E    +++    +T + GL+T  LK KR  + + F  ++  +Y
Sbjct:   21 NTVYEAFLRGMRMSDNGPFLGTRQATRG------------------YKWATYSQIHERASNFGSGLCELGCDPSQETFVGLWSTNCTEWLLADLACQMFSMVTVPIYDSHGSEACTYIINKAKLSTIVCHKK---KVNFLLEHAHLLPTLKSIIVISG---AGEVRRDKSDIL---------GIRIISFDEVEKIGIN---------NPHEKVPARPEDTFTISWTSGTTGFPKGVILTHDNIISDMSAYLFLIKQDGEELTP---QDTHLSYLPPEHMYERCTQVLMMMAGAKIGLYHGKREFLMD-----------DFKALRPTVFAAVPRLLNAIYDKV--VPLRRYSLFAGNTFFFHARRIIRKD-----TIWDRLVFKKIQDLIGGNVRIITCGSAPLSARVTSFIRCVMGCYLNEGYGSTETTAMVTMQRLKDFNTGHVGAPVPCATVKLVDVPEMDYYASNN---QGEICIRGRNVFKGYLHDAERTEEAF----DSSGWFYSGDIGEWTQDGTLRIIDRKKHIFKLSQGEYIAPAKIQNAYLRSMFVAQVFVHGNSLKSFIVAVVVPDAEVLIPWAKTHDI-EGDIKQLCQNQTVRKAIHGDMIAKGKEAKLNSLEQVKGIYLHPELFTVEEGLLTPTLKTKRPALAQFFEEKLNVLY 615

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Nematostella
Match: EDO46361 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQG4])

HSP 1 Score: 189.119 bits (479), Expect = 3.644e-51
Identity = 137/457 (29.98%), Postives = 224/457 (49.02%), Query Frame = 3

Query: 1011 PTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPI-----HRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYP------SVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFIN-ICFCPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQG--GEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            P  D L  + YTSG+T  PKG M+TH N+++ ++     L  +       DD ++ +LPL+H+ E   + V+  +G  +G+ +          IK     D   L+PT+   VP +L+RI   V  +   +      + RW ++  +N   R             D L+F KI+ LLGG+V +++SG APLS +   FI  +  C + +GYG TET  + TL +  D     VG P     +++ +  E +Y   ++   +GEI + G  V  GY   P  T E   +DKD  RW  TGDIG + + G+  +  R++    +QG  GEYI+  K+E    + PFV    V+ +S   F++  + P  + L   AEK  I   D++++C+ K V+  + + + K   E  L  +E    +H+    ++ D GL+T  LK+KR  +   F   I ++Y 
Sbjct:  108 PKSDDLYTVCYTSGTTGTPKGAMLTHDNVISDLSAFLYVLKQVIIRYFGVDDVHLSFLPLSHMYERVNQIVIFMNGGKIGFFN--------GDIK-KLLDDFKTLRPTVFGAVPRLLNRIHDKVTNEINKSW-----IKRWIFQIALNAKKRDLARKIVRRDTIWDYLIFNKIQALLGGRVWFIISGSAPLSPKVATFIRCVMGCQVQEGYGQTETVASVTLQLLDDFTAGHVGPPTPVNVVKLVDVPEMDYWANNK---QGEICVKGRNVFKGYLHNPAMTAE--VLDKD--RWLYTGDIGEWTTVGTRRLYTRERGAC-IQGNEGEYIAPQKIENIYLRSPFVAQIYVHGDSIRSFLVAVVVPDAEVLEAWAEKKGI-RGDLKRLCQNKTVHQRIFESMVKVGEESQLNSLEKVRGIHLCSEAFSIDNGLLTPTLKIKRPQMSTFFRDIIDNLYT 541

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Medaka
Match: acsl3a (acyl-CoA synthetase long chain family member 3 [Source:NCBI gene;Acc:101157383])

HSP 1 Score: 620.157 bits (1598), Expect = 0.000e+0
Identity = 312/676 (46.15%), Postives = 435/676 (64.35%), Query Frame = 3

Query:  318 PQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            P+ P+R + +   LV  M PG +TLD +   AA  +P++  LGTR+++  E E Q N K+FKK+    Y W +Y +     T  G GL +L  +P  + I IF ETR+EW+IAAQ CF YN P++TLYSTLG  AI HG+ E++ + +ITS+EL+  +L  I+ +   ++HII ++N               TT+    P  I++H M +V+ +G++      E+      +P    +A+IMYTSGST  PKGVM++H N++ ++ G  E +P +  DD YIGYLPLAHVLELC E+V ++HG  +GYSSP TL D+++KIK  +KGD S L+PT+MA VP I+DRI K+V  K  +   +   +F  AY YK++++ +G  +   D+LVF K++ LLGG+ R +LSGGAPLS   Q F+N+C C P+ QGYGLTETCGAGT+    D    +VG PL   +I++++W EG Y   D+P PRGEILIGG  VT+GY+K   K +EDFFVD++G RWFCTGDIG  H DG L IIDRKKDLVKLQ GEY+SL KVE A+  CP V+  CVYA S E +VI F+ P  +QLL +A +  I     +++C  + +   +LK + +A L   LER EIP K+ +    WTP+TGLVTDA KLKRK ++  +  +I+ MY 
Sbjct:   63 PEGPYRAVCASKSLVAVMRPGVDTLDKIFAYAATQFPHRDCLGTREVICEEDERQSNGKVFKKVVLGGYRWLSYQEAINAATQLGGGLAQLGQRPK-TNIAIFCETRAEWLIAAQACFAYNFPLVTLYSTLGGPAIAHGLNETQVSHIITSKELLETRLKAILPEVPRLQHIIVVDN-------------TPTTWPGY-PANISIHNMAAVQRLGAMP-----ENTACERKQPLPSDIAVIMYTSGSTGVPKGVMISHSNVIASITGIAEKIPNLSEDDTYIGYLPLAHVLELCAEHVCIAHGCRIGYSSPQTLADQSTKIKKGSKGDASILQPTLMAAVPEIMDRIYKNVMGKVEEMNSIQRTLFILAYNYKLDQLAKGRSTPLCDRLVFRKVRALLGGRTRVLLSGGAPLSAATQRFMNVCLCCPVGQGYGLTETCGAGTICELWDYSTGRVGGPLVCCEIKLKDWTEGGYRSTDKPHPRGEILIGGPNVTMGYYKSNAKQQEDFFVDENGQRWFCTGDIGEVHGDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLVDNICVYASSDETYVIGFMVPNQRQLLALAGQYGIRGSR-EELCNSRAIEELVLKAITEAALTAQLERFEIPRKIRLSSDPWTPETGLVTDAFKLKRKELKTHYKEDIERMYG 717

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Medaka
Match: acsl3b (long-chain-fatty-acid--CoA ligase 3 [Source:NCBI gene;Acc:101172360])

HSP 1 Score: 615.15 bits (1585), Expect = 0.000e+0
Identity = 325/728 (44.64%), Postives = 444/728 (60.99%), Query Frame = 3

Query:  159 PITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            P+ +   +   +VY   T+  +Y F      S   SN     R+ A  + +  P  P+R INS   LV  M PG NTLD +   AA  +P +  LGTR+++  E E QPN K+FKK+    Y W +Y +       FG GL  L  +P  + I IF ETR+EW++AAQ CF YN P++TLY+TLG +AI HG+ E+E T +ITS++L+  +L  I+     +++II +              D   T    +P  I ++ M +V+ MGS       E++ +    P    +A+IMYTSGST  PKGVM++H NL+  + G  E +P ++  D YIGYLPLAHVLEL  E V +SHG  +GYSSP TL D+++KIK  +KGD S LKPT+MA VP I+DRI K+V  K  +       +F  AY YKM ++ +GY +   D LVF +++ LLGG  R +LSGGAPLS   Q F+N+C C P+ QGYGLTETCGAGT+    D    +VG PL   +I +++W+EG Y   D+P PRGEILIGG  V +GY+K   K + DFFVD +G RWFCTGDIG FH DG L IIDRKKDLVKLQ GEY+SL KVE  +  CP ++  C YA S + +VI F+ P HKQL+ +AE+M I +   ++IC   ++   +L  + +A +   LER EIP K+ +    WTP+TGLVTDA KLKRK ++  +  +I+ MY
Sbjct:    9 PVLLQVFRSVVWVYSVITFLPWYFF------SGTGSNLERARRIKAYSV-SGHPAGPYRAINSQEKLVALMEPGVNTLDKMFEYAARRFPMRDCLGTREVLSEEDELQPNGKVFKKVILGNYNWLSYEEAYLAAKCFGSGLAALNQRPHCN-IAIFCETRAEWLVAAQACFMYNFPLVTLYATLGLKAIAHGLNETEVTHIITSKDLLQSRLKAILCDVPRLQYIIVV--------------DQKPTSWPDIPKSIKVYNMDAVKEMGS-----KPENISADRRHPQPSDIAVIMYTSGSTGIPKGVMISHSNLIAGITGMAERIPNLNETDTYIGYLPLAHVLELSAELVSISHGCRIGYSSPHTLADQSTKIKKGSKGDASVLKPTLMASVPEIMDRIYKNVMTKVEEMSRFQKTLFALAYNYKMEQISKGYSTPLCDSLVFKRVRSLLGGNTRVLLSGGAPLSAATQRFMNVCMCCPVGQGYGLTETCGAGTISEVWDYNTGRVGAPLVCSEITLKDWEEGGYYSTDKPNPRGEILIGGPNVAMGYYKYEGKNQGDFFVDANGQRWFCTGDIGEFHPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAVLKNCPLIDNICAYANSDQSYVISFVVPNHKQLMAVAEQMGI-KGTWEEICNNPQMEKEVLCIITEAAISGKLERFEIPKKIRLSAEPWTPETGLVTDAFKLKRKELKTHYQEDIERMY 708

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Medaka
Match: acsl4a (acyl-CoA synthetase long chain family member 4 [Source:NCBI gene;Acc:101166395])

HSP 1 Score: 610.912 bits (1574), Expect = 0.000e+0
Identity = 311/678 (45.87%), Postives = 432/678 (63.72%), Query Frame = 3

Query:  318 PQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNN--YIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICF-CPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            P+ P+R+++ F+ L     PG +TLD L   A   +     LGTR+++  E E Q N K+FKKL   +Y W +Y+++++ +  FG GL  L  +P  S I IF ETR+EWMI AQ CFR N P++T Y+TLG+EAI +G+ E+  T +ITS EL+  KL  ++ +  S+KH+I+++   Y  EGF                P+GI++  M +V  +G L      E++   +  P    LA++MYTSGST +PKGVM+ H NL+  M GQ E +P +   D YI YLPLAHVLE+  E   +++G  +GYSSP TL+D++++IK  +KGD S LKPT+MA VP I+DRI K+V  K  +   +   +F   Y+YK+ ++ +GY +   ++L+F K+KKLLGG VR MLSGGAPLS + Q F+N+CF CP+ QGYGLTETCGAGT+    D    +VG PL   +I++R+W EG Y+  D P PRGEILIGG  VT GYF+  +   +DFF D+ G RWFCTGDIG  H DG L I+DRKKDLVKLQ GEY+SL KVE A+  CP V+  C YA S ++FVI F+ P  K+L  +A++  I E   +++C   E+   +LK +    L+  L+R EIP KVH+    WTP+TGLVTDA KLKRK ++  +   I+ MY 
Sbjct:   21 PEGPYRSVDHFDSLAAEDFPGKDTLDKLFRHAVERFGPDPCLGTREILSEENETQSNGKVFKKLILGEYTWLSYNEVESIVNEFGSGLAALGQQPK-STIAIFCETRAEWMITAQACFRRNFPLVTFYATLGEEAIAYGLNETGITHLITSMELLDSKLKNVLPQIPSLKHVIYVDEKKYNAEGF----------------PEGISIRSMEAVRELGKLP-----ENMKKPIETPEPSDLAVVMYTSGSTGRPKGVMIVHSNLIAGMTGQCERIPDLGPKDTYIAYLPLAHVLEMTAEISCVTYGCRIGYSSPQTLSDQSTRIKKGSKGDCSVLKPTLMAAVPEIMDRINKNVMSKVQEMSYIQRTLFTLGYKYKLEQVKKGYDAPVCNRLLFGKVKKLLGGNVRMMLSGGAPLSSDTQRFMNVCFCCPVGQGYGLTETCGAGTITEVADISTGRVGAPLVCCEIRLRDWAEGGYTTKDTPNPRGEILIGGPNVTTGYFRH-EGNDDDFFQDEKGQRWFCTGDIGEVHPDGCLQIVDRKKDLVKLQAGEYVSLGKVESALKTCPLVDNICAYANSEQNFVISFVVPNQKKLTDLAKEKGI-EGTFEELCVHPEMEKEVLKRITDVALKIKLQRFEIPVKVHLSPEPWTPETGLVTDAFKLKRKELKNHYSDHIERMYG 674

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Query:  318 PQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNN--YIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICF-CPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            P+ P+R+++ F+ L     PG +TLD L   A   +     LGTR+++  E E Q N K+FKKL   +Y W +Y+++++ +  FG GL  L  +P  S I IF ETR+EWMI AQ CFR N P++T Y+TLG+EAI +G+ E+  T +ITS EL+  KL  ++ +  S+KH+I+++   Y  EGF                P+GI++  M +V  +G L      E++   +  P    LA++MYTSGST +PKGVM+ H NL+  M GQ E +P +   D YI YLPLAHVLE+  E   +++G  +GYSSP TL+D++++IK  +KGD S LKPT+MA VP I+DRI K+V  K  +   +   +F   Y+YK+ ++ +GY +   ++L+F K+KKLLGG VR MLSGGAPLS + Q F+N+CF CP+ QGYGLTETCGAGT+    D    +VG PL   +I++R+W EG Y+  D P PRGEILIGG  VT GYF+  +   +DFF D+ G RWFCTGDIG  H DG L I+DRKKDLVKLQ GEY+SL KVE A+  CP V+  C YA S ++FVI F+ P  K+L  +A++  I E   +++C   E+   +LK +    L+  L+R EIP KVH+    WTP+TGLVTDA KLKRK ++  +   I+ MY 
Sbjct:   21 PEGPYRSVDHFDSLAAEDFPGKDTLDKLFRHAVERFGPDPCLGTREILSEENETQSNGKVFKKLILGEYTWLSYNEVESIVNEFGSGLAALGQQPK-STIAIFCETRAEWMITAQACFRRNFPLVTFYATLGEEAIAYGLNETGITHLITSMELLDSKLKNVLPQIPSLKHVIYVDEKKYNAEGF----------------PEGISIRSMEAVRELGKLP-----ENMKKPIETPEPSDLAVVMYTSGSTGRPKGVMIVHSNLIAGMTGQCERIPDLGPKDTYIAYLPLAHVLEMTAEISCVTYGCRIGYSSPQTLSDQSTRIKKGSKGDCSVLKPTLMAAVPEIMDRINKNVMSKVQEMSYIQRTLFTLGYKYKLEQVKKGYDAPVCNRLLFGKVKKLLGGNVRMMLSGGAPLSSDTQRFMNVCFCCPVGQGYGLTETCGAGTITEVADISTGRVGAPLVCCEIRLRDWAEGGYTTKDTPNPRGEILIGGPNVTTGYFRH-EGNDDDFFQDEKGQRWFCTGDIGEVHPDGCLQIVDRKKDLVKLQAGEYVSLGKVESALKTCPLVDNICAYANSEQNFVISFVVPNQKKLTDLAKEKGI-EGTFEELCVHPEMEKEVLKRITDVALKIKLQRFEIPVKVHLSPEPWTPETGLVTDAFKLKRKELKNHYSDHIERMYG 674

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Medaka
Match: acsl3b (long-chain-fatty-acid--CoA ligase 3 [Source:NCBI gene;Acc:101172360])

HSP 1 Score: 595.504 bits (1534), Expect = 0.000e+0
Identity = 324/733 (44.20%), Postives = 440/733 (60.03%), Query Frame = 3

Query:  159 PITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELM-PKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGN-----LMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFC-PIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMY 2336
            P+ +   +   +VY   T+  +Y F      S   SN     R+ A  + +  P  P+R INS   LV  M PG NTLD +   AA  +P +  LGTR+++  E E QPN K+FKK+    Y W +Y +       FG GL  L  +P  + I IF ETR+EW++AAQ CF YN P++TLY+TLG +AI HG+ E+E T +ITS++L+  +L  I+     +++II +              D   T    +P  I ++ M +V+ MGS  +  D          P    +A+IMYTSGST  PKGVM++H       L++  +     +P IH  D YIGYLPLAHVLEL  E V +SHG  +GYSSP TL D+++KIK  +KGD S LKPT+MA VP I+DRI K+V  K  +       +F  AY YKM ++ +GY +   D LVF +++ LLGG  R +LSGGAPLS   Q F+N+C C P+ QGYGLTETCGAGT+    D    +VG PL   +I +++W+EG Y   D+P PRGEILIGG  V +GY+K   K + DFFVD +G RWFCTGDIG FH DG L IIDRKKDLVKLQ GEY+SL KVE  +  CP ++  C YA S + +VI F+ P HKQL+ +AE+M I +   ++IC   ++   +L  + +A +   LER EIP K+ +    WTP+TGLVTDA KLKRK ++  +  +I+ MY
Sbjct:    9 PVLLQVFRSVVWVYSVITFLPWYFF------SGTGSNLERARRIKAYSV-SGHPAGPYRAINSQEKLVALMEPGVNTLDKMFEYAARRFPMRDCLGTREVLSEEDELQPNGKVFKKVILGNYNWLSYEEAYLAAKCFGSGLAALNQRPHCN-IAIFCETRAEWLVAAQACFMYNFPLVTLYATLGLKAIAHGLNETEVTHIITSKDLLQSRLKAILCDVPRLQYIIVV--------------DQKPTSWPDIPKSIKVYNMDAVKEMGSKPENTDRR-------HPQPSDIAVIMYTSGSTGIPKGVMISHMCSFHFVLISQSSLTTVFIPNIH-TDTYIGYLPLAHVLELSAELVSISHGCRIGYSSPHTLADQSTKIKKGSKGDASVLKPTLMASVPEIMDRIYKNVMTKVEEMSRFQKTLFALAYNYKMEQISKGYSTPLCDSLVFKRVRSLLGGNTRVLLSGGAPLSAATQRFMNVCMCCPVGQGYGLTETCGAGTISEVWDYNTGRVGAPLVCSEITLKDWEEGGYYSTDKPNPRGEILIGGPNVAMGYYKYEGKNQGDFFVDANGQRWFCTGDIGEFHPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAVLKNCPLIDNICAYANSDQSYVISFVVPNHKQLMAVAEQMGI-KGTWEEICNNPQMEKEVLCIITEAAISGKLERFEIPKKIRLSAEPWTPETGLVTDAFKLKRKELKTHYQEDIERMY 710

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BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Planmine SMEST
Match: SMESG000081754.1 (SMESG000081754.1)

HSP 1 Score: 842.032 bits (2174), Expect = 0.000e+0
Identity = 404/731 (55.27%), Postives = 535/731 (73.19%), Query Frame = 3

Query:  162 ITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDK-YIGYLPLAHVLELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFCPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIE--EKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYNRH 2345
            I +N LKLW  +YEF T+PFY  F+ K       S N     V A PI+++D   PWRN+ + + L  T +PGCNTL  L  RA  LWP+  ALGTR+++ S+ E QPN + FKKLT  KYYWET+ + D RI N  + ++ L + P+  +++I++ET++EWMI AQ  F+ NI I TLY+TLG+EAIVHGI ESEA+++ TS EL+PK+  +I  C  +K I+FM+  IF+     + ++L    ++ +P  +TL  +  +E++G+      +E L +   KPT+DSLA+IMYTSGST  PKGV +T GN++ +MAG  + LP  + +++ YI YLPLAHV+EL CE ++++ GI +GYSSPLTLTD +SKIK+ TKGDIS L+PT+M CVPTIL+RITK+VW+K TD GP FLE+F+WAY+YK  R+ RGYPSVFMD++VF +IKKLLGG+V++MLSGGAPLSEEAQNF+NICFCPI+QGYGLTETC  G++M   D R    G P+ +LQI++R+W EG YSP D+P PRGE+LIGG  V LGY+KQP+KT+E FF D+ G RWFCTGDIG+   DG LSI+DRKKDLVKLQGGEY+SL KVELAISK P VE+ CVYA+SSE F ICFISPK KQL+Q+AEK  I+    D++ +CK K++  A+ K++ + C+EK +ER EIP KV +D   WTPD+GLVTDALKLKR+ I+ +F   I   YNR 
Sbjct:    8 IFLNILKLWVLIYEFITFPFYIFFKDK-------SENEI--VVSAVPIKDNDLSSPWRNVKALDKLATTPVPGCNTLVSLWERATILWPDNDALGTREVISSDFETQPNGRQFKKLTLGKYYWETFKETDERINNVAKAMINLGLVPTKDKVVIYSETQAEWMIVAQASFKSNIIITTLYATLGEEAIVHGINESEASLIYTSHELLPKIASVITSCPLIKTIVFMDQKIFKDRNAMEMEELKHKIKSKLPSNVTLMTLSKLEDIGA---ESKDEILKNLSNKPTKDSLAVIMYTSGSTGLPKGVKITQGNIVASMAGMKDVLPKFNVEEEIYISYLPLAHVMELVCELLLVTMGIKLGYSSPLTLTDTSSKIKSKTKGDISVLRPTLMVCVPTILERITKAVWDKVTDQGPFFLELFKWAYDYKRKRLGRGYPSVFMDRIVFRRIKKLLGGRVQFMLSGGAPLSEEAQNFVNICFCPIVQGYGLTETCAGGSIMNMYDLRTGHGGPPITSLQIKLRDWAEGGYSPQDKPYPRGEVLIGGGNVALGYYKQPEKTEECFFEDESGTRWFCTGDIGMISKDGCLSIVDRKKDLVKLQGGEYVSLGKVELAISKSPVVESVCVYADSSESFSICFISPKQKQLIQLAEKCGIDCSSNDLEVLCKDKKIIDAVTKDISQICIEKKIERFEIPQKVFLDPKPWTPDSGLVTDALKLKRREIKYKFQDVIDRFYNRE 726

[Close]

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Planmine SMEST
Match: SMESG000060730.1 (SMESG000060730.1)

HSP 1 Score: 715.301 bits (1845), Expect = 0.000e+0
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 3

Query:  144 MSILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLP 1172
            MSILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLP
Sbjct:    1 MSILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVLAKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSALGTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLKMKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLP 343

[Close]

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Planmine SMEST
Match: SMESG000060741.1 (SMESG000060741.1)

HSP 1 Score: 650.203 bits (1676), Expect = 0.000e+0
Identity = 313/313 (100.00%), Postives = 313/313 (100.00%), Query Frame = 3

Query: 1407 MFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFCPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYNRH 2345
            MFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFCPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYNRH
Sbjct:    1 MFRWAYEYKMNRMDRGYPSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFINICFCPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYNRH 313

[Close]

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Planmine SMEST
Match: SMESG000024739.1 (SMESG000024739.1)

HSP 1 Score: 206.453 bits (524), Expect = 7.738e-56
Identity = 175/639 (27.39%), Postives = 300/639 (46.95%), Query Frame = 3

Query:  456 LVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKL-KMKPSV-SRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGI--IESEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAM--AGQFEGLPPI-HRDDKYIGYLPLAHVLELCCENVVLSH-GIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGY--PSVFMDKLVFAKIKKLLGGKVRYMLSGGAPLSEEAQNFI-NICFCPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAEKMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            L+  E EP    K+    +   Y+W TYS++D      G G++KL    P + +++ IF +   EW+   Q C RY++ ++ LY T G+++  + I  ++ E   V T++++   L       H +K+I+ +N+      G +K Q+           G+   +   +   G              L  P     + I++TSG+T  PKG ++TH ++++++  A  F  +    ++ + ++ +LPL HV E    N++  + G    +S  + L  +  KI          +KPTI+  VP IL+RI   V    + + PL   +F      KM  ++RG    +   D ++F KI+ +LGG V  ++ G AP+S +  NF  ++    +I+GYG TET G  TL + GD     VGIP+    I++ +  E +   +     RGE+ + G      YF   D+      +D DG  W  TGDIG++   G L I+DR K + KL  GEY++  ++E   S  P V    V  +S +DF +  + P     L   + ++ + + ++ IC        + ++L     +  L   EI  +  +     +P+ GL+T  LKLKR    + F  EIK +Y 
Sbjct:   86 LIHGEHEPCLGYKV--DSSAANYHWLTYSEVDENSRQTGAGIIKLLNQSPGLNTKVGIFMKNCIEWVYVEQGCTRYSMLLVPLYDTSGEQSCQYIINHVKCEVIFVDTNKKVDFLLNSKSSMPH-LKYIVTLND-----IGKDKVQE-------ASLKGVQFFKFSDILATGKNNPIP--------LQLPKAQDPSSILFTSGTTGIPKGCLITHESMLSSIGSASTFADVIKFGNKKEIHLSFLPLTHVYERLVHNLMFMYTGKIAFFSGEIRLLAEEMKI----------IKPTILFAVPRILNRIYDKVMPIISQS-PLKSYIFNLGLAAKMKEVNRGIIRKNSIWDYIIFRKIQAILGGNVEIIMVGSAPVSPDVVNFFRSVMGSLVIEGYGQTETTGPATLTLPGDSDPGHVGIPVPGSFIKLCDVPELDL--VVARDCRGEVCVKGRMCISEYFG--DREMSSKLIDSDG--WLHTGDIGLWTEKGQLKIVDRCKHIFKLAQGEYLAPERLENIYSLSPLVAQIFVTGDSLKDFCVAIVIPD----LDGWKTVDAKYRSVEDICNDPVATEIIERDLSAVGRQNKLMGFEIVKRARLCSTAMSPENGLLTPTLKLKRAEASKFFAQEIKILYQ 680

[Close]

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Planmine SMEST
Match: SMESG000027204.1 (SMESG000027204.1)

HSP 1 Score: 146.362 bits (368), Expect = 2.420e-36
Identity = 145/553 (26.22%), Postives = 252/553 (45.57%), Query Frame = 3

Query:  756 VVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLITTFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMYTSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHV-LELCCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTILDRITKSVWEKATDNGPLFLEMFRWAYEY-KMNRMDR--GYP---SVFMDK-LVFAKIKKLLG-GKVRYMLSGGAPLSEEAQNFINICFCPIIQGYGLTETCGAGTLMVDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTLGYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQGGEYISLAKVELAI-SKCPFVETACVYAESSEDFVICFISPKH----------KQLLQIA----EKMNIEEKDIQKICKIKEVN-SALLKELQKACLEKGLERVEIPHKVHIDYLQWTPDTGLVTDALKLKRKNIQERFIVEIKDMYN 2339
            +V+ +++ + K+  I+     +K II         + G+ + +L T +       I+++      N+    D  DE  LD  L +   D  A+++YTSG+T  PKGVM++H N++  +    E +      +  I YLPL+H+   L      +S G+ V ++ P  L       K +    +  ++PT+   VP + ++I + + E  +  G +   +  WA      N + +  G P   S F+ K LVF KIK+ LG  +  Y+ S  APL++++ ++      PI Q YGL+E  G  T+ +    R+   G  L  ++++I E  E N          GEI I G  V LGY+   +KTKE   +D DG  W  +GD+G    +  L I  R K+L+   GGE I+   +E  I  + PF+ +  +  +  + FV C ++ K            QL   A    + + IE K ++++ + K++     + E  K      + R     K  +    ++   G +   +K+KR  I E +   +  MY+
Sbjct:   74 IVVENDDQLNKILSILPDVPQLKAII--------KYTGDVSAELKTKYS------ISIYTWTEFMNLNQ-ADSADE--LDRRLSEIQPDDCALLIYTSGTTGFPKGVMLSHDNMIFTVKKAMEHVKIQPFKNSVISYLPLSHIAANLNDIMTTVSVGVTVYFAQPDAL-------KGSLGKTLKEVRPTVFIGVPRVFEKIQEQMQEIGSKAGTIKKYIAGWAKNVGHRNSLAKITGKPVEKSYFLAKYLVFDKIKEALGLDRCIYLASAAAPLTQQSLDYFLSLDMPINQIYGLSECTGPHTMGITDKIRIGSAGTILKGVEMKIDEPNEHNI---------GEICIRGRHVFLGYYNNMEKTKES--IDSDG--WLHSGDLGKVEDNEHLYITGRIKELIITAGGENIAPVPIEDKIKERIPFISSVMLVGD-RQKFVSCLLTLKSTVDVDTQRPTNQLTPAAIRSLKDIGIEAKTVEEVLECKDIALKKFIDEAIKKVNSCSVSRAAYVQKWSLLPRDFSISGGELGPTMKMKRMAIVEMYSEIVSSMYS 588

The following BLAST results are available for this feature:

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Human
BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Celegans
BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Fly
BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Zebrafish
BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Xenopus
BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Mouse
BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. UniProt/SwissProt
BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. TrEMBL
BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Cavefish
BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Sea Lamprey
BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Yeast
BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Nematostella
BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Medaka
BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Planmine SMEST

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ACSL4	0.000e+0	46.65	acyl-CoA synthetase long chain family member 4 [So...	[more]
ACSL4	0.000e+0	46.65	acyl-CoA synthetase long chain family member 4 [So...	[more]
ACSL4	0.000e+0	46.65	acyl-CoA synthetase long chain family member 4 [So...	[more]
ACSL4	0.000e+0	46.65	acyl-CoA synthetase long chain family member 4 [So...	[more]
ACSL4	0.000e+0	48.40	acyl-CoA synthetase long chain family member 4 [So...	[more]

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
acs-4	0.000e+0	42.08	Fatty Acid CoA Synthetase family [Source:UniProtK...	[more]
acs-17	0.000e+0	42.59	Fatty Acid CoA Synthetase family [Source:UniProtK...	[more]
acs-17	0.000e+0	42.59	Fatty Acid CoA Synthetase family [Source:UniProtK...	[more]
acs-17	0.000e+0	42.59	Fatty Acid CoA Synthetase family [Source:UniProtK...	[more]
acs-3	1.281e-71	28.98	Fatty Acid CoA Synthetase family [Source:UniProtK...	[more]

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Acsl	0.000e+0	41.12	gene:FBgn0263120 transcript:FBtr0088700	[more]
Acsl	0.000e+0	41.12	gene:FBgn0263120 transcript:FBtr0100426	[more]
Acsl	0.000e+0	41.12	gene:FBgn0263120 transcript:FBtr0088699	[more]
Acsl	0.000e+0	41.12	gene:FBgn0263120 transcript:FBtr0100427	[more]
Acsl	0.000e+0	41.12	gene:FBgn0263120 transcript:FBtr0100430	[more]

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
acsl3b	0.000e+0	45.54	acyl-CoA synthetase long chain family member 3b [S...	[more]
acsl3b	0.000e+0	45.54	acyl-CoA synthetase long chain family member 3b [S...	[more]
acsl3b	0.000e+0	45.54	acyl-CoA synthetase long chain family member 3b [S...	[more]
acsl4a	0.000e+0	43.89	acyl-CoA synthetase long chain family member 4a [S...	[more]
acsl4a	0.000e+0	43.89	acyl-CoA synthetase long chain family member 4a [S...	[more]

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
acsl3	0.000e+0	47.60	acyl-CoA synthetase long chain family member 3 [So...	[more]
acsl3	0.000e+0	47.52	acyl-CoA synthetase long chain family member 3 [So...	[more]
acsl3	0.000e+0	45.26	acyl-CoA synthetase long chain family member 3 [So...	[more]
acsl3	0.000e+0	47.65	acyl-CoA synthetase long chain family member 3 [So...	[more]
acsl3	0.000e+0	49.57	acyl-CoA synthetase long chain family member 3 [So...	[more]

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Acsl4	0.000e+0	46.24	acyl-CoA synthetase long-chain family member 4 [So...	[more]
Acsl4	0.000e+0	46.24	acyl-CoA synthetase long-chain family member 4 [So...	[more]
Acsl4	0.000e+0	47.96	acyl-CoA synthetase long-chain family member 4 [So...	[more]
Acsl4	0.000e+0	47.96	acyl-CoA synthetase long-chain family member 4 [So...	[more]
Acsl3	0.000e+0	44.73	acyl-CoA synthetase long-chain family member 3 [So...	[more]

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|O60488\|ACSL4_HUMAN	0.000e+0	46.65	Long-chain-fatty-acid--CoA ligase 4 OS=Homo sapien...	[more]
sp\|Q9QUJ7\|ACSL4_MOUSE	0.000e+0	46.24	Long-chain-fatty-acid--CoA ligase 4 OS=Mus musculu...	[more]
sp\|O35547\|ACSL4_RAT	0.000e+0	46.10	Long-chain-fatty-acid--CoA ligase 4 OS=Rattus norv...	[more]
sp\|O95573\|ACSL3_HUMAN	0.000e+0	45.42	Long-chain-fatty-acid--CoA ligase 3 OS=Homo sapien...	[more]
sp\|Q5R668\|ACSL3_PONAB	0.000e+0	45.28	Long-chain-fatty-acid--CoA ligase 3 OS=Pongo abeli...	[more]

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A267GRF3	0.000e+0	51.55	AMP-binding domain-containing protein OS=Macrostom...	[more]
A0A267GYX3	0.000e+0	51.42	AMP-binding domain-containing protein OS=Macrostom...	[more]
A0A1I8GP46	0.000e+0	49.39	AMP-binding domain-containing protein OS=Macrostom...	[more]
C4QPH5	0.000e+0	44.24	Long-chain-fatty-acid--CoA ligase OS=Schistosoma m...	[more]
A0A094ZIV3	0.000e+0	44.68	Long-chain-fatty-acid--CoA ligase 3 OS=Schistosoma...	[more]

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ACSL3	0.000e+0	45.54	long-chain-fatty-acid--CoA ligase 3 [Source:NCBI g...	[more]
acsl4a	0.000e+0	43.46	acyl-CoA synthetase long chain family member 4 [So...	[more]
acsl3a	0.000e+0	43.85	acyl-CoA synthetase long chain family member 3a [S...	[more]
acsl4b	4.913e-180	42.16	acyl-CoA synthetase long chain family member 4b [S...	[more]
acsl2	7.840e-75	31.23	long-chain-fatty-acid--CoA ligase 6 [Source:NCBI g...	[more]

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
acsl3b	1.387e-74	47.25	acyl-CoA synthetase long chain family member 3b [S...	[more]
ACSL1	2.223e-72	30.36	acyl-CoA synthetase long chain family member 1 [So...	[more]
ACSL1	3.374e-62	32.05	acyl-CoA synthetase long chain family member 1 [So...	[more]
acsbg2	1.679e-22	22.12	acyl-CoA synthetase bubblegum family member 2 [Sou...	[more]
ENSPMAT00000005646.1	9.300e-11	31.31	pep scaffold:Pmarinus_7.0:GL484188:1842:9754:1 gen...	[more]

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5


Match Name	E-value	Identity	Description
FAA1	1.278e-95	31.73	Long chain fatty acyl-CoA synthetase; activates fa...	[more]
FAA3	1.309e-89	32.47	Long chain fatty acyl-CoA synthetase; activates im...	[more]
FAA4	4.714e-79	29.10	Long chain fatty acyl-CoA synthetase; activates fa...	[more]
FAA2	2.165e-50	26.52	Medium chain fatty acyl-CoA synthetase; activates ...	[more]
PCS60	1.324e-10	25.28	Oxalyl-CoA synthetase; capable of catalyzing conve...	[more]

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO29879	0.000e+0	46.82	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO45461	7.063e-68	29.36	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO46335	8.823e-67	28.17	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO45504	1.032e-62	27.78	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO46361	3.644e-51	29.98	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
acsl3a	0.000e+0	46.15	acyl-CoA synthetase long chain family member 3 [So...	[more]
acsl3b	0.000e+0	44.64	long-chain-fatty-acid--CoA ligase 3 [Source:NCBI g...	[more]
acsl4a	0.000e+0	45.87	acyl-CoA synthetase long chain family member 4 [So...	[more]
acsl4a	0.000e+0	45.87	acyl-CoA synthetase long chain family member 4 [So...	[more]
acsl3b	0.000e+0	44.20	long-chain-fatty-acid--CoA ligase 3 [Source:NCBI g...	[more]

BLAST of Long-chain-fatty-acid--CoA ligase 4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000081754.1	0.000e+0	55.27	SMESG000081754.1	[more]
SMESG000060730.1	0.000e+0	100.00	SMESG000060730.1	[more]
SMESG000060741.1	0.000e+0	100.00	SMESG000060741.1	[more]
SMESG000024739.1	7.738e-56	27.39	SMESG000024739.1	[more]
SMESG000027204.1	2.420e-36	26.22	SMESG000027204.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30031771 ID=SMED30031771|Name=Long-chain-fatty-acid--CoA ligase 4|organism=Schmidtea mediterranea sexual|type=transcript|length=3026bp
GTTTAATCATTAAATCTGCTTTAAAAATCGTATCATAGTTATATTGGTTA
ATTTTCAGAATTAGAAATTTTTACATTCGCATTTAATTTTATAATCATTC
ATAATTTATATTATTGTAACAAGAATTCCAATTTAAATAAAGAATGAGTA
TCTTGATTCCGATCACAGTCAACGCTTTAAAATTATGGACATATGTCTAT
GAATTTTTCACCTGGCCATTTTATTATATTTTTCAATCTAAATGCACATT
GTCATCAATTAACAGTAATAATAATTGCGATGGTAGAGTTCTTGCTAAAC
CAATACAAAATGATGATCCTCAAGAACCGTGGAGAAATATAAATTCGTTT
AATGGTCTAGTCAATACAATGATTCCCGGGTGCAATACACTAGATGATTT
AATGATACGCGCAGCTAATTTGTGGCCTAATAAATCGGCTCTTGGTACAA
GAAAACTCGTTAAAAGTGAAATGGAGCCACAACCTAATAAAAAGTTATTT
AAAAAATTAACGTTTGAAAAGTATTATTGGGAAACATATAGCGATATTGA
TACAAGAATTACAAATTTTGGTCGCGGATTGTTAAAATTAAAGATGAAGC
CTAGTGTAAGTCGGATTTTGATTTTTGCCGAAACCCGAAGTGAATGGATG
ATCGCAGCTCAAACATGTTTTAGATACAACATTCCAATAATAACTCTCTA
CTCGACTCTGGGCGATGAAGCCATTGTTCATGGAATTATTGAATCGGAAG
CCACAGTTGTCATTACCAGCGAAGAGTTGATGCCGAAACTTTGCAAAATA
ATCGAAAAATGTCATTCAGTGAAACACATAATATTCATGAACAACTATAT
TTTCGAGGGTTTTGGCGGCAATAAAGCTCAAGATTTGATTACGACTTTTG
AAAATGTGGTCCCAGACGGCATTACGTTGCATGAGATGGGATCGGTCGAG
AATATGGGATCTCTAAAGGATTATGGTGATGAAGAATCTCTGGATTCGTA
TCTATACAAACCGACCAGGGACAGTCTCGCCATGATTATGTACACAAGTG
GATCCACTGATAAACCAAAGGGTGTCATGATGACTCATGGCAATCTAATG
ACTGCAATGGCGGGGCAATTCGAGGGCTTGCCACCGATCCATAGAGATGA
TAAATATATAGGTTATTTACCCCTAGCACATGTACTTGAGTTGTGCTGTG
AGAATGTTGTTCTTTCACATGGAATACCTGTAGGATATTCAAGTCCATTG
ACATTAACTGACAAGGCTAGCAAAATCAAAGCCAATACTAAAGGCGATAT
TTCGGCACTCAAACCAACAATAATGGCATGCGTGCCAACCATTTTGGATA
GAATTACTAAATCCGTATGGGAAAAGGCTACAGATAATGGACCTCTATTT
CTCGAGATGTTCCGTTGGGCATATGAATATAAAATGAATCGTATGGATCG
CGGATATCCTAGTGTTTTTATGGACAAATTAGTTTTCGCTAAAATAAAAA
AATTACTTGGTGGTAAAGTTAGATACATGCTTAGTGGCGGTGCTCCTCTT
AGCGAAGAAGCTCAAAACTTTATTAACATTTGTTTTTGTCCTATTATTCA
AGGATACGGATTAACGGAAACATGCGGAGCAGGTACGTTAATGGTTGACG
GGGACCGTAGAGTTAATCAGGTTGGGATCCCCCTCAATGCTTTGCAAATC
CAAATTAGAGAATGGAAGGAAGGTAATTATTCTCCACTGGATCAACCGAA
ACCCAGAGGAGAAATTCTAATAGGTGGTGAATGTGTAACTTTGGGTTATT
TTAAACAGCCAGACAAAACTAAAGAAGACTTTTTTGTTGATAAAGATGGC
ATGCGTTGGTTTTGCACAGGTGATATCGGCGTGTTTCACTCCGATGGTAG
TCTTTCCATTATCGACCGTAAAAAAGATTTAGTGAAACTACAAGGTGGAG
AATACATATCACTGGCTAAAGTTGAACTTGCTATTTCAAAATGCCCTTTT
GTTGAAACTGCATGTGTCTATGCCGAAAGTTCAGAGGATTTTGTAATTTG
CTTTATTTCTCCTAAACACAAGCAACTTTTGCAAATTGCCGAAAAAATGA
ACATAGAAGAAAAAGATATACAGAAAATTTGTAAAATAAAAGAGGTTAAT
TCAGCATTGCTTAAAGAATTACAAAAAGCTTGTTTGGAAAAAGGACTAGA
AAGGGTGGAGATACCACATAAAGTCCACATTGATTACCTTCAATGGACAC
CTGACACCGGTCTAGTTACCGATGCTTTAAAATTGAAACGTAAAAATATT
CAAGAAAGATTCATTGTGGAAATCAAGGATATGTATAATCGTCATTAATA
ATTATTATTTTATAAATCTATACATATATAGTGCATTTTCGATTTTTATA
TATTCCCAATGCAACACATGTGCAATATACTTTCGTGAGTTGTTTGTTAG
TTTTATTCCATGACATGAAAATGTTCATGCTGTTTGTTTCTATGTTCTAG
TAGCATTCTTTAGTCATTGAGTTATTATTTGATGCTGACGTCACACTTAT
TATAATTAATTATTATTATTGTTGTTGTTATTATTACTATTGCTATTGAT
AAATTTTGACAGGTTACTCCAATGTAAGTATAAAAAGTGAATTTGATTAG
TACTGTTTATCGGTGGTGTATGATTGGTGTTTGTTTTTTCAGTTTGAATA
CTAATTTTTTTTCTTCAATAATCAAACATTCTCATTAAATTCGATTGATT
TCCCATTATAGCGTTTATATTGAAAGTTGTGCTTGCTACTTTTGCACGAA
TAATAATAACAATATTTTAATATTATATAGTGCTTTTATATGGATATACA
TATATTATGTTTATATATTGATTGATAATTAAATAAATAAATTACACATG
TGCGCGATTAATAGGAAAGATGTTTTTGTGAATTTTTACACACATGTTTA
TCAAAAATTCTGTTGGGAAATCGATTTTTTTAAATTCAAAAATAATTACG
AGAATTTTCCACCAATGAGAATTCTT

protein sequence of SMED30031771-orf-1

>SMED30031771-orf-1 ID=SMED30031771-orf-1|Name=SMED30031771-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=735bp
MSILIPITVNALKLWTYVYEFFTWPFYYIFQSKCTLSSINSNNNCDGRVL
AKPIQNDDPQEPWRNINSFNGLVNTMIPGCNTLDDLMIRAANLWPNKSAL
GTRKLVKSEMEPQPNKKLFKKLTFEKYYWETYSDIDTRITNFGRGLLKLK
MKPSVSRILIFAETRSEWMIAAQTCFRYNIPIITLYSTLGDEAIVHGIIE
SEATVVITSEELMPKLCKIIEKCHSVKHIIFMNNYIFEGFGGNKAQDLIT
TFENVVPDGITLHEMGSVENMGSLKDYGDEESLDSYLYKPTRDSLAMIMY
TSGSTDKPKGVMMTHGNLMTAMAGQFEGLPPIHRDDKYIGYLPLAHVLEL
CCENVVLSHGIPVGYSSPLTLTDKASKIKANTKGDISALKPTIMACVPTI
LDRITKSVWEKATDNGPLFLEMFRWAYEYKMNRMDRGYPSVFMDKLVFAK
IKKLLGGKVRYMLSGGAPLSEEAQNFINICFCPIIQGYGLTETCGAGTLM
VDGDRRVNQVGIPLNALQIQIREWKEGNYSPLDQPKPRGEILIGGECVTL
GYFKQPDKTKEDFFVDKDGMRWFCTGDIGVFHSDGSLSIIDRKKDLVKLQ
GGEYISLAKVELAISKCPFVETACVYAESSEDFVICFISPKHKQLLQIAE
KMNIEEKDIQKICKIKEVNSALLKELQKACLEKGLERVEIPHKVHIDYLQ
WTPDTGLVTDALKLKRKNIQERFIVEIKDMYNRH*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **cellular component**
Term	Definition
GO:0016020	membrane
GO:0016021	integral component of membrane

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000026	gut
PLANA:0003116	parenchymal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR000873	AMP-dep_Synth/Lig

Vocabulary: **biological process**
Term	Definition
GO:0008152	metabolic process
GO:0001676	long-chain fatty acid metabolic process

Vocabulary: **molecular function**
Term	Definition
GO:0003824	catalytic activity
GO:0004467	long-chain fatty acid-CoA ligase activity
GO:0016874	ligase activity
GO:0102391	decanoate--CoA ligase activity

Main menu

Long-chain-fatty-acid--CoA ligase 4

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 9

Owlery

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