GCR158

Overview
NameGCR158
Smed IDSMED30031760
Length (bp)1106
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of GCR158 (SMED30031760) t-SNE clustered cells

Violin plots show distribution of expression levels for GCR158 (SMED30031760) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of GCR158 (SMED30031760) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for GCR158 (SMED30031760) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30031760

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30031760SMESG000062349.1 Contig5586GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30031760SMESG000062349.1 dd_Smed_v4_8379_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031760SMESG000062349.1 dd_Smed_v4_8379_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of GCR158 vs. TrEMBL
Match: A0A193KUF9 (GCR158 OS=Schmidtea mediterranea OX=79327 GN=gcr158 PE=2 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 3.132e-21
Identity = 116/318 (36.48%), Postives = 188/318 (59.12%), Query Frame = 3
Query:   69 DTNKKTIEIAFYPIYSGMFILSVLLNSILLYVVTKIESYAHEKRVFHKXXXXXXXXXXXXXXXXXXXXXXKRFTEDTDTNIFLDVFLAIRDLSISAQMITLVLIAIDRLFAFVNPLSN--YWVLNTVMWILKVLMLFLSLSYITRLILLSIGISCLNXXXXXXXXXXXXXXXXXVFMLINLVIYVSLFSLLCQRQLVRQRNSFNSDKFYRAMQSKSYDENPEENETSITKSRRISSLNSRKLRFRRRIGSIKFAKVLLGINILMIVSYLPGVLYNNKFIPFPSYSVY-YLFFLHALGDPIIFLLTRRELKNGLLSILK 1013
            D N K  E   + I+  +F+LSV LNSILLY+V K   +  +++  H LFQ LI  DL+ISILILP+IL+K+   +   +  + +F A+RD  IS Q+  LV IAID L + +NP+++  +W L+ +  I    +  + +  + + ILL   +    K+ I+   K  + + G +F+++ ++IY  LF L+ +RQ+ R   S    +  ++ QS   +EN  E+E+   K      +  ++  F +RI  +KF KVL  +N+LMIVS+LPG LYN+  + F +Y+ + YLF++H+LGD IIFL  R +L+  L  +L+
Sbjct:    8 DANIKEGEAIIFSIFIIIFLLSVFLNSILLYLVMKKNYFKFDQQSIHYLFQMLIVCDLLISILILPDILVKKTVTNGKYHPAVILFRAMRDALISIQIFLLVAIAIDGLVSILNPINSRQFWNLSNIKRIFITYLSVIVVLLLVKSILLLSNLDSNVKQNINYAIKFGLSLIGMIFIVMIIIIYSYLFCLIYRRQMDRCDKSMKRKQCLKS-QSTETEENFSEDESGRNK------ITVKQWNFGKRIIHMKFVKVLFWVNLLMIVSFLPGCLYNSGLLKFKAYNWFIYLFYVHSLGDAIIFLFIRLDLRKDLTKLLR 318          
BLAST of GCR158 vs. TrEMBL
Match: A0A193KUR6 (GCR156 OS=Schmidtea mediterranea OX=79327 GN=gcr156 PE=2 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 6.682e-18
Identity = 74/238 (31.09%), Postives = 126/238 (52.94%), Query Frame = 3
Query:  291 EDTDTNIFLDVFLAIRDLSISAQMITLVLIAIDRLFAFVNPLSNYWVLNTVMWILKVLMLFLSLSYITRLILLSIGISCLNXXXXXXXXXXXXXXXXXVFMLINLVIYVSLFSLLCQRQLVRQRNSFNSDKFYRAMQSKSYDENPEENETSITKSRRISSLNSRKLRFRRRIG-SIKFAKVLLGINILMIVSYLPGVLYNNKFIPFPSYSVYYLFFLHALGDPIIFLLTRRELKNGLL 1001
            E+   NI L+ FL++R+  IS   ITL+ +A +R  A  +P       + ++ + +++ + +S+ ++ R  L+     CL  ++ID   K+ IGI+  + +++ + IY  LF LLC R       +F S        SK +    E   T++ K             F+R  G S+KFA+VL  I ILM++SY P  L N   I +P  ++YYLF+L  LGDP+I +L+R +++  ++
Sbjct:   87 ENRSENILLEGFLSLRNCLISCHAITLLFMATNRYRAIKHPQMVNDPRSVLIKLYRIIYVTISIPFLVRFALVFCLRDCLLLQEIDNALKMSIGIATALVVMVIICIYSVLFGLLCCR-------NFPSTSKPTGNFSKIHPYPNERKSTTVGKV------------FKRHFGKSVKFAQVLFSITILMMISYAPVFLTNVNLIKYPGQAIYYLFYLPVLGDPLILILSREDIRQEMI 305          
BLAST of GCR158 vs. Planmine SMEST
Match: SMESG000062349.1 (SMESG000062349.1)

HSP 1 Score: 559.296 bits (1440), Expect = 0.000e+0
Identity = 327/327 (100.00%), Postives = 327/327 (100.00%), Query Frame = 3
Query:   39 MNLTETCRNHDTNKKTIEIAFYPIYSGMFILSVLLNSILLYVVTKIESYAHEKRVFHKXXXXXXXXXXXXXXXXXXXXXXKRFTEDTDTNIFLDVFLAIRDLSISAQMITLVLIAIDRLFAFVNPLSNYWVLNTVMWILKVLMLFLSLSYITRLILLSIGISCLNXXXXXXXXXXXXXXXXXVFMLINLVIYVSLFSLLCQRQLVRQRNSFNSDKFYRAMQSKSYDENPEENETSITKSRRISSLNSRKLRFRRRIGSIKFAKVLLGINILMIVSYLPGVLYNNKFIPFPSYSVYYLFFLHALGDPIIFLLTRRELKNGLLSILKRK 1019
            MNLTETCRNHDTNKKTIEIAFYPIYSGMFILSVLLNSILLYVVTKIESYAHEKRVFHKLFQFLIFLDLIISILILPNILIKRFTEDTDTNIFLDVFLAIRDLSISAQMITLVLIAIDRLFAFVNPLSNYWVLNTVMWILKVLMLFLSLSYITRLILLSIGISCLNKKKIDKYGKIIIGISGGVFMLINLVIYVSLFSLLCQRQLVRQRNSFNSDKFYRAMQSKSYDENPEENETSITKSRRISSLNSRKLRFRRRIGSIKFAKVLLGINILMIVSYLPGVLYNNKFIPFPSYSVYYLFFLHALGDPIIFLLTRRELKNGLLSILKRK
Sbjct:    1 MNLTETCRNHDTNKKTIEIAFYPIYSGMFILSVLLNSILLYVVTKIESYAHEKRVFHKLFQFLIFLDLIISILILPNILIKRFTEDTDTNIFLDVFLAIRDLSISAQMITLVLIAIDRLFAFVNPLSNYWVLNTVMWILKVLMLFLSLSYITRLILLSIGISCLNKKKIDKYGKIIIGISGGVFMLINLVIYVSLFSLLCQRQLVRQRNSFNSDKFYRAMQSKSYDENPEENETSITKSRRISSLNSRKLRFRRRIGSIKFAKVLLGINILMIVSYLPGVLYNNKFIPFPSYSVYYLFFLHALGDPIIFLLTRRELKNGLLSILKRK 327          
BLAST of GCR158 vs. Planmine SMEST
Match: SMESG000062328.1 (SMESG000062328.1)

HSP 1 Score: 271.166 bits (692), Expect = 1.103e-88
Identity = 181/329 (55.02%), Postives = 232/329 (70.52%), Query Frame = 3
Query:   51 ETCRNHDTNKKTIEIAFYPIYSGMFILSVLLNSILLYVVTKIESYAHEKRVFHKXXXXXXXXXXXXXXXXXXXXXXKRFTEDTDTNIFLDVFLAIRDLSISAQMITLVLIAIDRLFAFVNPLSNYWVLNTVMWILKVLMLFLSLSYITRLILLSIGISCLNXXXXXXXXXXXXXXXXXVFMLINLVIYVSLFSLLCQRQLVRQRNSFNSDKF----YRAMQSKSYD--ENPEENETSITKSRRISSLNSRKLRFRRRIGSIKFAKVLLGINILMIVSYLPGVLYNNKFIPFPSYSVYYLFFLHALGDPIIFLLTRRELKNGLLSILKRK 1019
             TCR HD  K  I+ AFYPIYS MF+LSV LNSILLY+V K + + HEK+ FHKLFQ LIFLDLIISILILPNILIK +++D DT I LD +LAIRD +IS+QMI LVLIAIDRL AF+ PLSN+W +  V+ + K+  +   L Y  R  LL++ I+C  KK ID   K+ IGI GG+ M+INL+IY  LF+LLC+RQ+   ++S  S  F     + ++SKS    EN + +  ++      S +  + +   R+IGSIKF KVL G+N+LMI+SYLP +LYN + I FPS+SVYYLFFLHALGDPI+F +TRREL N LL I+  K
Sbjct:   13 NTCRKHDIEKNGIDGAFYPIYSIMFVLSVFLNSILLYIVIKKDKFIHEKKSFHKLFQVLIFLDLIISILILPNILIKMYSKDHDTFIVLDGYLAIRDFAISSQMIILVLIAIDRLLAFLLPLSNFWRIGRVLKVFKIFFILTLLFYSIRFTLLTVNINCDIKKSIDSDVKLFIGIFGGILMVINLIIYCILFALLCRRQIFNHKDSLLSANFKSLNVKELESKSIPKIENKKNSSNAMLLQSDFSQVKFKVIPANRKIGSIKFVKVLFGVNLLMIISYLPAILYNAECIQFPSFSVYYLFFLHALGDPILFFMTRRELLNSLLKIVHIK 341          
BLAST of GCR158 vs. Planmine SMEST
Match: SMESG000077660.1 (SMESG000077660.1)

HSP 1 Score: 216.468 bits (550), Expect = 1.562e-67
Identity = 163/323 (50.46%), Postives = 210/323 (65.02%), Query Frame = 3
Query:   54 TCRNHDTNKKTIEIAFYPIYSGMFILSVLLNSILLYVVTKIESYAHEKRVFHKXXXXXXXXXXXXXXXXXXXXXXKRFTEDTDTNIFLDVFLAIRDLSISAQMITLVLIAIDRLFAFVNPLSNYWVLNTVMWILKVLMLFLSLSYITRLILLSIGISCLNXXXXXXXXXXXXXXXXXVFMLINLVIYVSLFSLLCQRQLVRQRNSFNSDKFYRAMQSKSYDENPE----ENETSITKSRRISSLNSRKLRFRRRIGSIKFAKVLLGINILMIVSYLPGVLYNNKFIPFPSYSVYYLFFLHALGDPIIFLLTRRELKNGLLSIL 1010
            TCR+HD  K+ IE+AF+P+Y+ MF ++V+LNS+LL++V K      EKR F KLF  LIFLDLIISILILPNI IK++T DTDT I +D +LA+RD SIS+QM   V IAIDRL    +P       NT++ +L+  + F+ L Y+ R I L+I I C +K  +DKY K+ IGI GGV M  NL+IY+ LF LLC RQ    R    S K  R   S+      +    E     T ++R SSL ++     ++  SI+FAK L  IN+LMI+SYLP +LYN +F+ F SYSVYYLFFLHALGDPI+F  TR ELK  LL  L
Sbjct:    6 TCRSHDVKKEDIEVAFFPLYASMFAVAVILNSVLLHLVVKKNRLFLEKRTFDKLFPVLIFLDLIISILILPNIFIKKYTNDTDTYIAVDGYLAVRDFSISSQMTIFVAIAIDRLLTLTHPQITKRRTNTIIKVLQTAVFFIGLFYLLRFIFLTIDIKCNSKVLLDKYMKLFIGIVGGVLMTTNLIIYLVLFGLLCHRQRKESRKFQKSKKLLRTQNSEENVNENQENLIEIPMRPTNNKR-SSLKNKFKS-NKKSASIRFAKPLFAINLLMIISYLPAILYNVRFVGFHSYSVYYLFFLHALGDPILFFSTRPELKLALLRCL 326          
BLAST of GCR158 vs. Planmine SMEST
Match: SMESG000012027.1 (SMESG000012027.1)

HSP 1 Score: 140.198 bits (352), Expect = 2.526e-38
Identity = 123/327 (37.61%), Postives = 195/327 (59.63%), Query Frame = 3
Query:   45 LTETCRNHDTNKKTIEIAFYPIYSGMFILSVLLNSILLYVVTKIESYAHEKRVFHKXXXXXXXXXXXXXXXXXXXXXXKRFTEDTDTNIFLDVFLAIRDLSISAQMITLVLIAIDRLFAFVNPLSNYWVLNTVMWILKVLMLFLSLSYITRLILLSIGISCLNXXXXXXXXXXXXXXXXXVFMLINLVIYVSLFSLLCQRQLVRQRNSFNSDKFYRAMQSKSYDENP-EENETSITKSRRISSLNSRKLRFRRRIGSIKFAKVLLGINILMIVSYLPGVLYNNKFIPFPSYS-VYYLFFLHALGDPIIFLLTRRELKNGLLSILKRK 1019
            +TE C+ + T     +  F  I+  +F+LSV LNS LL +VTK   +  +K+  HKLFQ LI LDL+ISILILPN+LIKRF +D  T++ +DVF ++RD  IS Q+  L+LIA+D L + + P+  +W LN++        + +SL +I +L++ ++ +SC   KKI++Y K+ +G+ GG+ M   L IYV LF L+ ++Q          D+  +  + +     P   NE    + +R S     KL   ++I  +KF KVL  +N+LM++S+LP  LYN+  + +  +    YLF++H+LGD IIFL  R  +KN +  + ++K
Sbjct:    4 ITEECQRNVTESVESKTVFASIFIFIFLLSVTLNSFLLVLVTKRNYFDFDKQASHKLFQILIALDLVISILILPNVLIKRFAKDP-THLAVDVFRSVRDSVISIQVFLLLLIAVDGLVSILYPIQTFWNLNSITKTFASYSVVISLIFIVKLVVFNVNLSCGLVKKINRYFKLTMGVIGGIIMATILSIYVCLFVLIFRQQF---------DRHVKFPKQRPTSPLPVRRNEERNFQDQRTSKFGLEKLMLGQKILQMKFVKVLFLVNLLMVISFLPVSLYNSGLLRYSYFKWASYLFYIHSLGDAIIFLFVRFNMKNDMTKVFRKK 320          
BLAST of GCR158 vs. Planmine SMEST
Match: SMESG000062345.1 (SMESG000062345.1)

HSP 1 Score: 125.561 bits (314), Expect = 2.129e-34
Identity = 86/133 (64.66%), Postives = 104/133 (78.20%), Query Frame = 3
Query:  129 LSVLLNSILLYVVTKIESYAHEKRVFHKXXXXXXXXXXXXXXXXXXXXXXKRFTEDTDTNIFLDVFLAIRDLSISAQMITLVLIAIDRLFAFVNPLSNYWVLNTVMWILKVLMLFLSLSYITRLILLSIGISC 527
            + VL NS+LLYVVT    + H+KR FHKLFQ LI LDLIIS+LILPNI+IK FT+DTDT+ + D+FLA RD +IS+QMITLVLIAIDRL AF  P+S  W L  VMWIL+VL++ + LSY  R ILL+I ISC
Sbjct:   18 MKVLFNSLLLYVVTNRVKFCHDKRTFHKLFQILIILDLIISLLILPNIIIKGFTKDTDTHWYTDLFLASRDFAISSQMITLVLIAIDRLLAFFVPMSKIWKLEIVMWILEVLLVIIFLSYGVRFILLTINISC 150          
The following BLAST results are available for this feature:
BLAST of GCR158 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 2
Match NameE-valueIdentityDescription
A0A193KUF93.132e-2136.48GCR158 OS=Schmidtea mediterranea OX=79327 GN=gcr15... [more]
A0A193KUR66.682e-1831.09GCR156 OS=Schmidtea mediterranea OX=79327 GN=gcr15... [more]
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BLAST of GCR158 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000062349.10.000e+0100.00SMESG000062349.1[more]
SMESG000062328.11.103e-8855.02SMESG000062328.1[more]
SMESG000077660.11.562e-6750.46SMESG000077660.1[more]
SMESG000012027.12.526e-3837.61SMESG000012027.1[more]
SMESG000062345.12.129e-3464.66SMESG000062345.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031760 ID=SMED30031760|Name=GCR158|organism=Schmidtea mediterranea sexual|type=transcript|length=1106bp
CATAAATCAAATAATTTGGACATCTAACATTTACAAACATGAATCTTACA
GAAACATGCCGTAATCATGATACCAATAAAAAAACTATCGAAATTGCTTT
TTATCCAATTTATTCTGGCATGTTTATTCTTTCAGTTTTACTCAATTCAA
TATTACTTTATGTGGTCACTAAAATAGAGAGTTATGCTCACGAAAAGAGA
GTTTTTCATAAATTATTTCAATTTCTCATTTTTCTTGATTTGATAATTTC
GATTTTAATTTTACCAAATATTTTAATCAAGCGATTTACAGAGGATACGG
ATACTAATATTTTTCTCGATGTGTTTCTAGCGATTCGTGATTTGTCTATT
TCAGCGCAAATGATTACATTGGTTCTAATTGCTATTGATCGACTGTTTGC
ATTTGTGAATCCGTTGAGTAACTATTGGGTATTGAATACAGTTATGTGGA
TTTTAAAAGTTTTGATGCTTTTTCTTTCATTATCCTACATCACACGTTTA
ATTCTTCTCAGCATTGGCATCTCATGCTTGAATAAAAAGAAAATTGATAA
ATATGGAAAAATAATTATCGGGATTTCTGGTGGTGTATTTATGTTAATTA
ATTTAGTAATTTATGTATCTTTATTCTCACTTTTATGCCAACGCCAATTG
GTTCGACAAAGAAACTCATTTAACTCCGATAAATTTTATAGAGCAATGCA
AAGTAAATCGTATGATGAAAATCCAGAAGAAAATGAAACTTCAATAACAA
AATCTAGACGTATTTCATCTTTAAATTCTCGAAAATTAAGGTTTCGGCGA
AGAATAGGATCGATTAAATTTGCGAAAGTTCTTCTTGGAATTAATATATT
GATGATTGTATCCTACTTACCAGGTGTATTATATAATAACAAATTCATTC
CTTTCCCATCATATTCGGTATATTATTTATTTTTTCTCCATGCACTTGGA
GATCCAATAATATTTTTATTGACACGTCGTGAACTGAAAAATGGTTTATT
ATCTATATTAAAAAGAAAGTAAATATTCAAAACACATTTTTATTTAAATC
AGTTACATTTCTCAAAGGATTCTTAAAGATTTTATTTAAGTTTTCTCAAA
ATTACG
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protein sequence of SMED30031760-orf-1

>SMED30031760-orf-1 ID=SMED30031760-orf-1|Name=SMED30031760-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=328bp
MNLTETCRNHDTNKKTIEIAFYPIYSGMFILSVLLNSILLYVVTKIESYA
HEKRVFHKLFQFLIFLDLIISILILPNILIKRFTEDTDTNIFLDVFLAIR
DLSISAQMITLVLIAIDRLFAFVNPLSNYWVLNTVMWILKVLMLFLSLSY
ITRLILLSIGISCLNKKKIDKYGKIIIGISGGVFMLINLVIYVSLFSLLC
QRQLVRQRNSFNSDKFYRAMQSKSYDENPEENETSITKSRRISSLNSRKL
RFRRRIGSIKFAKVLLGINILMIVSYLPGVLYNNKFIPFPSYSVYYLFFL
HALGDPIIFLLTRRELKNGLLSILKRK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000276G protein-coupled receptor, rhodopsin-likePRINTSPR00237GPCRRHODOPSNcoord: 100..122
score: 25.38
coord: 20..44
score: 17.99
coord: 291..317
score: 16.35
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 42..280
e-value: 8.0E-11
score: 41.8
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 5..327
e-value: 8.7E-20
score: 72.8
NoneNo IPR availablePANTHERPTHR24246OLFACTORY RECEPTOR AND ADENOSINE RECEPTORcoord: 14..324
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 9..327
NoneNo IPR availableTMHMMTMhelixcoord: 21..43
NoneNo IPR availableTMHMMTMhelixcoord: 176..198
NoneNo IPR availableTMHMMTMhelixcoord: 292..311
NoneNo IPR availableTMHMMTMhelixcoord: 58..80
NoneNo IPR availableTMHMMTMhelixcoord: 261..282
NoneNo IPR availableTMHMMTMhelixcoord: 139..161
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 35..309
score: 11.545