SMED30031746

Overview
NameSMED30031746
Smed IDSMED30031746
Length (bp)962
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30031746 (SMED30031746) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30031746 (SMED30031746) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30031746

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30031746h1SMcG0008407 SmedASXL_029817SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30031746h1SMcG0008407 dd_Smed_v4_24183_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30031746h1SMcG0008407 dd_Smed_v4_24183_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30031746 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.003990:26814..27774 -4694
v31.001301:48295..49588 -4610
Homology
[Close]
BLAST of SMED30031746 vs. Ensembl Human
Match: TMEM216 (transmembrane protein 216 [Source:HGNC Symbol;Acc:HGNC:25018])

HSP 1 Score: 50.0618 bits (118), Expect = 5.740e-7
Identity = 42/141 (29.79%), Postives = 71/141 (50.35%), Query Frame = -3 
Query:  493 SPYFENVKSSRFVHLFFCYHMWYNLLYFSVEFLIFVYKQRNLRYQSEAYTXXXXXXXXXXLCEIGRLYHSVRSNLDENVKLVVHSFILTGICLIVFIYMCLWQSYVLRIELILNSFQISFYIIEMXXXXXXXIVFRKQEKL 915
            +P  + + S+    LFF  + WYN  YF +E  IF+YK   L Y +    L++++L   L  E+ RL+   + NL +    +  S  LT    ++  Y  L Q+YVLR+E I+N   + F   E++  +  L  F   +++
Sbjct:    9 APRGKRLSSTPLEILFFL-NGWYNATYFLLELFIFLYKGVLLPYPTANLVLDVVMLLLYLGIEVIRLFFGTKGNLCQRKMPLSISVALTFPSAMMASYYLLLQTYVLRLEAIMNGILLFFCGSELLLEVLTLAAFSSMDRI 148
[Close]
BLAST of SMED30031746 vs. Ensembl Human
Match: TMEM216 (transmembrane protein 216 [Source:HGNC Symbol;Acc:HGNC:25018])

HSP 1 Score: 49.2914 bits (116), Expect = 9.614e-7
Identity = 38/115 (33.04%), Postives = 60/115 (52.17%), Query Frame = -3 
Query:  571 SPYFENVKSSRFVHLFFCYHMWYNLLYFSVEFLIFVYKQRNLRYQSEAYTXXXXXXXXXXLCEIGRLYHSVRSNLDENVKLVVHSFILTGICLIVFIYMCLWQSYVLRIELILNS 915
            +P  + + S+    LFF  + WYN  YF +E  IF+YK   L Y +    L++++L   L  E+ RL+   + NL +    +  S  LT    ++  Y  L Q+YVLR+E I+N 
Sbjct:    9 APRGKRLSSTPLEILFFL-NGWYNATYFLLELFIFLYKGVLLPYPTANLVLDVVMLLLYLGIEVIRLFFGTKGNLCQRKMPLSISVALTFPSAMMASYYLLLQTYVLRLEAIMNG 122
[Close]
BLAST of SMED30031746 vs. Ensembl Zebrafish
Match: TMEM216 (si:rp71-1d10.5 [Source:NCBI gene;Acc:555988])

HSP 1 Score: 51.6026 bits (122), Expect = 1.008e-7
Identity = 39/136 (28.68%), Postives = 71/136 (52.21%), Query Frame = -3 
Query:  499 ENVKSSRFVHLFFCYHMWYNLLYFSVEFLIFVYKQRNLRYQSEAYTXXXXXXXXXXLCEIGRLYHSVRSNL-DENVKLVVHSFILTGICLIVFIYMCLWQSYVLRIELILNSFQISFYIIEMXXXXXXXIVFRKQE 903
            + V SS  + + F  + WY   +F  E L+F+YK   L Y  +   L++++L      E  RL++  + NL   ++ L V   IL   C ++ +Y  L Q++VLR+E +LN+  + FY  E++  +  + +F +  
Sbjct:   24 QPVLSSTPLQILFHLNGWYFAAFFVAEILMFIYKGVILPYPQDNLILDVVLLLLFSGLETLRLFYGWKGNLCQRSLALFVSVAILVP-CAVLSVYYLLLQTFVLRLEFVLNAVLLCFYGFELVLGVMTISIFSRAN 158
[Close]
BLAST of SMED30031746 vs. Ensembl Zebrafish
Match: TMEM216 (si:rp71-1d10.5 [Source:NCBI gene;Acc:555988])

HSP 1 Score: 50.0618 bits (118), Expect = 2.709e-7
Identity = 38/130 (29.23%), Postives = 69/130 (53.08%), Query Frame = -3 
Query:  505 SSRFVHLFFCYHMWYNLLYFSVEFLIFVYKQRNLRYQSEAYTXXXXXXXXXXLCEIGRLYHSVRSNL-DENVKLVVHSFILTGICLIVFIYMCLWQSYVLRIELILNSFQISFYIIEMXXXXXXXIVFRK 891
            SS  + + F  + WY   +F  E L+F+YK   L Y  +   L++++L      E  RL++  + NL   ++ L V   IL   C ++ +Y  L Q++VLR+E +LN+  + FY  E++  +  + +F +
Sbjct:   13 SSTPLQILFHLNGWYFAAFFVAEILMFIYKGVILPYPQDNLILDVVLLLLFSGLETLRLFYGWKGNLCQRSLALFVSVAILVP-CAVLSVYYLLLQTFVLRLEFVLNAVLLCFYGFELVLGVMTISIFSR 141
[Close]
BLAST of SMED30031746 vs. Ensembl Xenopus
Match: pkd1 (polycystin 1, transient receptor potential channel interacting [Source:Xenbase;Acc:XB-GENE-483409])

HSP 1 Score: 55.0694 bits (131), Expect = 7.190e-9
Identity = 45/123 (36.59%), Postives = 64/123 (52.03%), Query Frame = -3 
Query:  544 PYFENVKSSRFVHLFFCYHMWYNLLYFSVEFLIFVYKQRNLRYQSEAYTXXXXXXXXXXLCEIGRLYHSVRSNLDENVKLVVHSFILTGICLIVFIYMCLWQSYVLRIELILNSFQISFYIIE 912
            P  + + S+    LFF  + WY   YF +E LIF+YK   L Y +    LE  +LF  L  E+ RL+   R NL E    +V S  LT    ++ +Y  L Q+Y LR+E +LN   + FY +E
Sbjct:    9 PEHKQLSSTPLEVLFF-LNGWYCASYFLIETLIFIYKGLILPYPTSNLILESFLLFLYLGIEVSRLFLGCRGNLCERKLPLVVSLGLTIPAGLLAVYFLLLQTYALRLETVLNCILLLFYGLE 130
[Close]
BLAST of SMED30031746 vs. Ensembl Mouse
Match: Tmem216 (transmembrane protein 216 [Source:MGI Symbol;Acc:MGI:1920020])

HSP 1 Score: 48.9062 bits (115), Expect = 7.173e-7
Identity = 43/141 (30.50%), Postives = 72/141 (51.06%), Query Frame = -3 
Query:  493 SPYFENVKSSRFVHLFFCYHMWYNLLYFSVEFLIFVYKQRNLRYQSEAYTXXXXXXXXXXLCEIGRLYHSVRSNLDENVKLVVHSFILTGICLIVFIYMCLWQSYVLRIELILNSFQISFYIIEMXXXXXXXIVFRKQEKL 915
            +P  + + S+    LFF  + WY   YF +E LIF+YK   L Y +    L++++L   L  E+ RL+   + NL +    +  S  LT    ++  Y  L Q+YVLR+E I+NS  + F   E++  +  L  F   +++
Sbjct:    2 APRDKRLSSTPLEVLFFL-NGWYYATYFLLELLIFLYKGLLLPYPTANLVLDVVMLLLYLGIEVIRLFFGTKGNLCQRKMPLGISVALTFPSAMMASYYLLLQTYVLRLEAIMNSILLFFCGSELLLEMLTLATFSSMDRI 141
[Close]
BLAST of SMED30031746 vs. Ensembl Mouse
Match: Tmem216 (transmembrane protein 216 [Source:MGI Symbol;Acc:MGI:1920020])

HSP 1 Score: 48.9062 bits (115), Expect = 7.173e-7
Identity = 43/141 (30.50%), Postives = 72/141 (51.06%), Query Frame = -3 
Query:  493 SPYFENVKSSRFVHLFFCYHMWYNLLYFSVEFLIFVYKQRNLRYQSEAYTXXXXXXXXXXLCEIGRLYHSVRSNLDENVKLVVHSFILTGICLIVFIYMCLWQSYVLRIELILNSFQISFYIIEMXXXXXXXIVFRKQEKL 915
            +P  + + S+    LFF  + WY   YF +E LIF+YK   L Y +    L++++L   L  E+ RL+   + NL +    +  S  LT    ++  Y  L Q+YVLR+E I+NS  + F   E++  +  L  F   +++
Sbjct:    2 APRDKRLSSTPLEVLFFL-NGWYYATYFLLELLIFLYKGLLLPYPTANLVLDVVMLLLYLGIEVIRLFFGTKGNLCQRKMPLGISVALTFPSAMMASYYLLLQTYVLRLEAIMNSILLFFCGSELLLEMLTLATFSSMDRI 141
[Close]
BLAST of SMED30031746 vs. UniProt/SwissProt
Match: sp|E7EYQ9|TM216_DANRE (Transmembrane protein 216 OS=Danio rerio OX=7955 GN=tmem216 PE=3 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 7.609e-7
Identity = 39/136 (28.68%), Postives = 71/136 (52.21%), Query Frame = -3 
Query:  499 ENVKSSRFVHLFFCYHMWYNLLYFSVEFLIFVYKQRNLRYQSEAYTXXXXXXXXXXLCEIGRLYHSVRSNL-DENVKLVVHSFILTGICLIVFIYMCLWQSYVLRIELILNSFQISFYIIEMXXXXXXXIVFRKQE 903
            + V SS  + + F  + WY   +F  E L+F+YK   L Y  +   L++++L      E  RL++  + NL   ++ L V   IL   C ++ +Y  L Q++VLR+E +LN+  + FY  E++  +  + +F +  
Sbjct:   24 QPVLSSTPLQILFHLNGWYFAAFFVAEILMFIYKGVILPYPQDNLILDVVLLLLFSGLETLRLFYGWKGNLCQRSLALFVSVAILVP-CAVLSVYYLLLQTFVLRLEFVLNAVLLCFYGFELVLGVMTISIFSRAN 158
[Close]
BLAST of SMED30031746 vs. UniProt/SwissProt
Match: sp|Q9P0N5|TM216_HUMAN (Transmembrane protein 216 OS=Homo sapiens OX=9606 GN=TMEM216 PE=1 SV=3)

HSP 1 Score: 49.2914 bits (116), Expect = 4.617e-6
Identity = 38/115 (33.04%), Postives = 60/115 (52.17%), Query Frame = -3 
Query:  571 SPYFENVKSSRFVHLFFCYHMWYNLLYFSVEFLIFVYKQRNLRYQSEAYTXXXXXXXXXXLCEIGRLYHSVRSNLDENVKLVVHSFILTGICLIVFIYMCLWQSYVLRIELILNS 915
            +P  + + S+    LFF  + WYN  YF +E  IF+YK   L Y +    L++++L   L  E+ RL+   + NL +    +  S  LT    ++  Y  L Q+YVLR+E I+N 
Sbjct:    9 APRGKRLSSTPLEILFFL-NGWYNATYFLLELFIFLYKGVLLPYPTANLVLDVVMLLLYLGIEVIRLFFGTKGNLCQRKMPLSISVALTFPSAMMASYYLLLQTYVLRLEAIMNG 122
[Close]
BLAST of SMED30031746 vs. UniProt/SwissProt
Match: sp|B6ID01|TM216_RAT (Transmembrane protein 216 OS=Rattus norvegicus OX=10116 GN=Tmem216 PE=2 SV=2)

HSP 1 Score: 49.2914 bits (116), Expect = 4.645e-6
Identity = 41/133 (30.83%), Postives = 68/133 (51.13%), Query Frame = -3 
Query:  493 SSRFVHLFFCYHMWYNLLYFSVEFLIFVYKQRNLRYQSEAYTXXXXXXXXXXLCEIGRLYHSVRSNLDENVKLVVHSFILTGICLIVFIYMCLWQSYVLRIELILNSFQISFYIIEMXXXXXXXIVFRKQEKL 891
            SS  + + F  + WY   YF +E LIF+YK   L Y +    L++++L   L  E+ RL+   + NL +    +  S  LT    ++  Y  L Q+YVLR+E I+NS  + F   E++  +  L  F   +++
Sbjct:    9 SSTPLEILFFLNGWYYATYFLLELLIFLYKGLLLPYPTANLVLDVVMLLLYLGIEVIRLFFGTKGNLCQRKMPLGISVALTFPSAMMASYYLLLQTYVLRLEAIMNSILLFFCGSELLLEMLTLATFSSMDRI 141
[Close]
BLAST of SMED30031746 vs. UniProt/SwissProt
Match: sp|Q9CQC4|TM216_MOUSE (Transmembrane protein 216 OS=Mus musculus OX=10090 GN=Tmem216 PE=1 SV=2)

HSP 1 Score: 48.9062 bits (115), Expect = 5.017e-6
Identity = 43/141 (30.50%), Postives = 72/141 (51.06%), Query Frame = -3 
Query:  493 SPYFENVKSSRFVHLFFCYHMWYNLLYFSVEFLIFVYKQRNLRYQSEAYTXXXXXXXXXXLCEIGRLYHSVRSNLDENVKLVVHSFILTGICLIVFIYMCLWQSYVLRIELILNSFQISFYIIEMXXXXXXXIVFRKQEKL 915
            +P  + + S+    LFF  + WY   YF +E LIF+YK   L Y +    L++++L   L  E+ RL+   + NL +    +  S  LT    ++  Y  L Q+YVLR+E I+NS  + F   E++  +  L  F   +++
Sbjct:    2 APRDKRLSSTPLEVLFFL-NGWYYATYFLLELLIFLYKGLLLPYPTANLVLDVVMLLLYLGIEVIRLFFGTKGNLCQRKMPLGISVALTFPSAMMASYYLLLQTYVLRLEAIMNSILLFFCGSELLLEMLTLATFSSMDRI 141
[Close]
BLAST of SMED30031746 vs. TrEMBL
Match: A0A3B4ERF5 (Uncharacterized protein OS=Pygocentrus nattereri OX=42514 PE=4 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 2.641e-10
Identity = 40/117 (34.19%), Postives = 64/117 (54.70%), Query Frame = -3 
Query:  553 ENVKSSRFVHLFFCYHMWYNLLYFSVEFLIFVYKQRNLRYQSEAYTXXXXXXXXXXLCEIGRLYHSVRSNLDENVKLVVHSFILTGICLIVFIYMCLWQSYVLRIELILNSFQISFY 903
             +V SS  + +  C   +Y+L YF     + +YK + L Y      L++ +L  +   +I R+Y  VR NL E+ K V  + I+TG  L+  +Y  +WQSYVLR ++I+NS  +  Y
Sbjct:   10 ASVLSSVLLQILLCLSAFYSLFYFLASLCLIIYKSQVLSYPDSDLALDVCLLLLMAALDIIRVYLGVRGNLQESEKYVGANLIITGTTLLFAVYFLVWQSYVLRADVIVNSILLCLY 126
[Close]
BLAST of SMED30031746 vs. TrEMBL
Match: A0A5S6QFH0 (Uncharacterized protein OS=Trichuris muris OX=70415 PE=4 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 1.094e-9
Identity = 47/137 (34.31%), Postives = 72/137 (52.55%), Query Frame = -3 
Query:  541 FNVSFLLKF*MKSPYFENVKSSRFVHLFFCYHMWYNLLYFSVEFLIFVYKQRNLRYQSEAYTXXXXXXXXXXLCEIGRLYHSVRSNLDENVKLVVHSFILTGICLIVFIYMCLWQSYVLRIELILNSFQISFYIIEM 951
            F+ + +     K    E ++SS  + LF  +H WY LL+F  E   FVYK  +LRY +  +  E L+L  LL  E+ R       NL E+  LV  S  L   C++ + Y+  +Q+YV+R+E++L    + F I EM
Sbjct:   20 FDAAAVAAMLPKPGGKEILRSSFALQLFLYFHGWYYLLFFIGETAAFVYKGISLRYPTNLFAYEALLLLLLLGMEVARNSFGRSGNLTESFSLVAVSLFLAAPCVLAYGYLLYYQTYVVRLEVVLTIVSLLFIISEM 156
[Close]
BLAST of SMED30031746 vs. TrEMBL
Match: A0A3M7R578 (Transmembrane protein-like OS=Brachionus plicatilis OX=10195 GN=BpHYR1_031458 PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.137e-9
Identity = 41/121 (33.88%), Postives = 70/121 (57.85%), Query Frame = -3 
Query:  496 HMWYNLLYFSVEFLIFVYKQRNLRYQSEAYTXXXXXXXXXXLCEIGRLYHSVRSNLDENVKLVVHSFILTGICLIVFIYMCLWQSYVLRIELILNSFQISFYIIEMXXXXXXXIVFRKQEK 858
            + WY + +  VE L FV+K + L + S     E+++   L   ++ R++ + + NL E +  +V S ILT  C+   +Y+ +WQ YVLR+ELI+N+ Q+ F ++E I  I  +IVF +   
Sbjct:   20 NQWYYIFWLIVESLCFVFKGQTLPFSSGVLAGEIILFIFLFFLDMFRIFFATKGNLTERIFGIVVSIILTAPCVGGSLYLLIWQHYVLRVELIINAIQLVFILLEFILEIIAIIVFARTSS 140
[Close]
BLAST of SMED30031746 vs. TrEMBL
Match: K1R3X3 (Transmembrane protein 80 OS=Crassostrea gigas OX=29159 GN=CGI_10007099 PE=4 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.656e-8
Identity = 40/119 (33.61%), Postives = 67/119 (56.30%), Query Frame = -3 
Query:  541 VKSSRFVHLFFCYHMWYNLLYFSVEFLIFVYKQRNLRYQSEAYTXXXXXXXXXXLCEIGRLYHSVRSNLDENVKLVVHSFILTGICLIVFIYMCLWQSYVLRIELILNSFQISFYIIEM 897
            V+SS    +    + WY   +F  E LI+VYK   L Y       E++++F L   E  RL+ + + NL E +  VV S +L+   ++  +Y+ LWQ+YVLR+++IL + Q +F  +E+
Sbjct:   18 VRSSLPYQVLLYLNGWYFAFFFLCEILIYVYKNETLPYADNVLPAEVILVFILAGIEALRLFFAQKGNLTERIVGVVISILLSIPAVLGALYLILWQTYVLRVDVILAAIQAAFIGLEL 136
[Close]
BLAST of SMED30031746 vs. TrEMBL
Match: A7SXC1 (Predicted protein OS=Nematostella vectensis OX=45351 GN=v1g175269 PE=4 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 1.096e-7
Identity = 45/148 (30.41%), Postives = 84/148 (56.76%), Query Frame = -3 
Query:  493 MKSPYFENVK-SSRFVHLFFCYHMWYNLLYFSVEFLIFVYK----QRNLRYQSEAYTXXXXXXXXXXLCEIGRLYHSVRSNLDENVKLVVHSFILTGICLIVFIYMCLWQSYVLRIELILNSFQISFYIIEMXXXXXXXIVFRKQEKL 921
            M +P    ++ SS  + +F   ++WY++++   E L+++YK       L Y S A T+E+L++      E  RL+   + NL E    ++ S +L    L + +++ LWQ+YVLRIE+IL +  ++F  IE+I  I+ ++ F+K + +
Sbjct:    1 MAAPGRTVIELSSLPLQIFLYLNVWYSMVFCVAEILLYIYKGIVLSSLLPYTSAALTVEILLVIFWAFMEGVRLFFGYKGNLAERSMALIISIVLGIPVLFIELFVLLWQTYVLRIEVILVAISLAFLGIEIILSIAAMLTFKKYQSI 148
[Close]
BLAST of SMED30031746 vs. Ensembl Cavefish
Match: ENSAMXT00000052229.1 (transmembrane protein 80 [Source:NCBI gene;Acc:103025724])

HSP 1 Score: 55.0694 bits (131), Expect = 4.194e-9
Identity = 36/117 (30.77%), Postives = 60/117 (51.28%), Query Frame = -3 
Query:  553 ENVKSSRFVHLFFCYHMWYNLLYFSVEFLIFVYKQRNLRYQSEAYTXXXXXXXXXXLCEIGRLYHSVRSNLDENVKLVVHSFILTGICLIVFIYMCLWQSYVLRIELILNSFQISFY 903
             +V SS  + +       Y L YF     + +YK + L Y      L++ +L  +   ++ RLY  VR NL E+ K +  +   TG  +++ +Y  +WQSYVLR ++I+NS  +  Y
Sbjct:   10 SSVLSSVLLQILLYLSSIYGLFYFISSLCLIIYKSQVLSYPDSDLALDVCLLLLMAGLDVIRLYWGVRGNLLESEKYISANLFSTGATVLLAVYFLVWQSYVLRADVIINSILLCLY 126
[Close]
BLAST of SMED30031746 vs. Ensembl Cavefish
Match: si:rp71-1d10.5 (transmembrane protein 216 [Source:HGNC Symbol;Acc:HGNC:25018])

HSP 1 Score: 48.521 bits (114), Expect = 9.390e-7
Identity = 39/129 (30.23%), Postives = 68/129 (52.71%), Query Frame = -3 
Query:  505 SSRFVHLFFCYHMWYNLLYFSVEFLIFVYKQRNLRYQSEAYTXXXXXXXXXXLCEIGRLYHSVRSNLDENVKLVVHSFILTGICLIVFIYMCLWQSYVLRIELILNSFQISFYIIEMXXXXXXXIVFRK 891
            SS  + + F  + WY  ++F  E L+F+YK   L Y      L++++L   L  E  RL++  + NL E    +  S  +   C ++ +Y  L Q++VLR+E ILN+  + FY +E+I  +  +  F +
Sbjct:    7 SSTPLQVLFYLNSWYFAVFFIAEILMFIYKGVLLPYPQSNLILDIVLLLLYLGLETLRLFYGWKGNLCERTLTLSISIGVLVPCTVLSVYYLLLQTFVLRLEFILNAVLLCFYGLELILGLITISAFAR 135
[Close]
BLAST of SMED30031746 vs. Ensembl Nematostella
Match: EDO31652 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SXC1])

HSP 1 Score: 60.8474 bits (146), Expect = 1.516e-11
Identity = 45/148 (30.41%), Postives = 84/148 (56.76%), Query Frame = -3 
Query:  493 MKSPYFENVK-SSRFVHLFFCYHMWYNLLYFSVEFLIFVYK----QRNLRYQSEAYTXXXXXXXXXXLCEIGRLYHSVRSNLDENVKLVVHSFILTGICLIVFIYMCLWQSYVLRIELILNSFQISFYIIEMXXXXXXXIVFRKQEKL 921
            M +P    ++ SS  + +F   ++WY++++   E L+++YK       L Y S A T+E+L++      E  RL+   + NL E    ++ S +L    L + +++ LWQ+YVLRIE+IL +  ++F  IE+I  I+ ++ F+K + +
Sbjct:    1 MAAPGRTVIELSSLPLQIFLYLNVWYSMVFCVAEILLYIYKGIVLSSLLPYTSAALTVEILLVIFWAFMEGVRLFFGYKGNLAERSMALIISIVLGIPVLFIELFVLLWQTYVLRIEVILVAISLAFLGIEIILSIAAMLTFKKYQSI 148
The following BLAST results are available for this feature:
BLAST of SMED30031746 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
TMEM2165.740e-729.79transmembrane protein 216 [Source:HGNC Symbol;Acc:... [more]
TMEM2169.614e-733.04transmembrane protein 216 [Source:HGNC Symbol;Acc:... [more]
back to top
BLAST of SMED30031746 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30031746 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30031746 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
TMEM2161.008e-728.68si:rp71-1d10.5 [Source:NCBI gene;Acc:555988][more]
TMEM2162.709e-729.23si:rp71-1d10.5 [Source:NCBI gene;Acc:555988][more]
back to top
BLAST of SMED30031746 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pkd17.190e-936.59polycystin 1, transient receptor potential channel... [more]
back to top
BLAST of SMED30031746 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Tmem2167.173e-730.50transmembrane protein 216 [Source:MGI Symbol;Acc:M... [more]
Tmem2167.173e-730.50transmembrane protein 216 [Source:MGI Symbol;Acc:M... [more]
back to top
BLAST of SMED30031746 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|E7EYQ9|TM216_DANRE7.609e-728.68Transmembrane protein 216 OS=Danio rerio OX=7955 G... [more]
sp|Q9P0N5|TM216_HUMAN4.617e-633.04Transmembrane protein 216 OS=Homo sapiens OX=9606 ... [more]
sp|B6ID01|TM216_RAT4.645e-630.83Transmembrane protein 216 OS=Rattus norvegicus OX=... [more]
sp|Q9CQC4|TM216_MOUSE5.017e-630.50Transmembrane protein 216 OS=Mus musculus OX=10090... [more]
back to top
BLAST of SMED30031746 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3B4ERF52.641e-1034.19Uncharacterized protein OS=Pygocentrus nattereri O... [more]
A0A5S6QFH01.094e-934.31Uncharacterized protein OS=Trichuris muris OX=7041... [more]
A0A3M7R5781.137e-933.88Transmembrane protein-like OS=Brachionus plicatili... [more]
K1R3X31.656e-833.61Transmembrane protein 80 OS=Crassostrea gigas OX=2... [more]
A7SXC11.096e-730.41Predicted protein OS=Nematostella vectensis OX=453... [more]
back to top
BLAST of SMED30031746 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSAMXT00000052229.14.194e-930.77transmembrane protein 80 [Source:NCBI gene;Acc:103... [more]
si:rp71-1d10.59.390e-730.23transmembrane protein 216 [Source:HGNC Symbol;Acc:... [more]
back to top
BLAST of SMED30031746 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30031746 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30031746 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO316521.516e-1130.41Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of SMED30031746 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30031746 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031746 ID=SMED30031746|Name=SMED30031746|organism=Schmidtea mediterranea sexual|type=transcript|length=962bp
AAAATTAAAAACGTTAAAAGGTAAAGTACAATAATAAAGCAAAAAGATCA
TCAAGCAATTATATTATAATATATCGAAACGATAATGAAATAAATTAAAT
TGGAATTAAATGACTGCAACGAGATTTAGCAGGGTAACATAATAAAGCCT
CGAGAAACTTGGTATAGGTAATTGGTCTTGTGTAACTCCCTTCACAGCTA
CACCTGTTCCTGTGACGGCGGGTCTTTATTGAGTAGGTAATGCTATTCAA
TGTAGCTGGCAGTTAAGTGGCACACTCCAGAGTGACCCAAACGACTCTGC
AGTACATATCAGAAACTAATCGAAACTACAATAAGAAAAAACACAATAAC
AAGAAAAACAATTTCACCAAATAAATGAACTTATGCTTATAATTTCTTTT
ATTATTAAGTATAAGTAAAATTTTAATAAAATTATAGACAAATTATTTTA
TTTATACAGAATAAAATCTAACAAATCATTTTATTCATAAGATAACTTCT
CCTGTTTTCTAAATACAATCAACAACGAAATTAATGATATCATTTCAATG
ATATAAAAAGATATTTGAAAAGAATTGAGAATTAATTCGATTCTTAATAC
ATAAGATTGCCACAAGCACATATAAATAAATACTATCAGACAAATACCAG
TTAATATAAAGGAATGAACGACTAATTTTACATTTTCATCCAAATTAGAT
CTAACTGAATGATATAAACGTCCAATTTCACATAATAATAATGAAAATAG
AATCAATAACTCCAAAGTATATGCTTCAGATTGATATCTTAAATTTCTTT
GCTTGTAGACAAATATTAAAAATTCAACTGAAAAATATAACAAATTGTAC
CACATATGGTAACAAAAAAACAAATGTACAAATCTTGAACTTTTAACATT
TTCAAAATAAGGAGATTTCATTTAAAACTTTAACAAAAAACTTACATTAA
AAAATTTATTTT
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR019184Uncharacterised_TM-17
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019184Uncharacterised protein family, transmembrane-17PFAMPF09799Transmemb_17coord: 18..123
e-value: 2.6E-20
score: 73.0
IPR019184Uncharacterised protein family, transmembrane-17PANTHERPTHR13531FAMILY NOT NAMEDcoord: 8..143
NoneNo IPR availableTMHMMTMhelixcoord: 83..105
NoneNo IPR availableTMHMMTMhelixcoord: 15..37
NoneNo IPR availableTMHMMTMhelixcoord: 115..137