SMED30031708
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30031708 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Homology
BLAST of SMED30031708 vs. Planmine SMEST
Match: SMESG000065442.1 (SMESG000065442.1) HSP 1 Score: 174.481 bits (441), Expect = 8.083e-51 Identity = 115/150 (76.67%), Postives = 130/150 (86.67%), Query Frame = 1 Query: 1 DNQQQRINMMLDNGGVRXXXXXXXXXXXXXXXXXLQREEAREFQERWKSRDQNTRHINMMMVLNDEEIHTAEQ-CFLNSAGDKIDEHELNEKEDVIPTKRFKTIELSSEEKIKRIQEFIKEKKSEKVTNKRNTKKVEEIQNEETQKIPKP 447 DNQQQRINM+ DNG VRQ NTNNNQEQQKT WQ LQREEARE QERWK+R QNTRHI+MM V+NDEEI+TAEQ C +N+AGDKIDE ELNEKED+IPTK KTIELSSEE+IKRIQEFIKEKK ++ + KK+EEIQNEET+K+PKP Sbjct: 201 DNQQQRINMIQDNGDVRQKNTNNNQEQQKTEWQRLQREEAREIQERWKTRGQNTRHISMMRVVNDEEINTAEQQCVVNNAGDKIDEQELNEKEDLIPTKIIKTIELSSEERIKRIQEFIKEKKEKRKEIREIQKKIEEIQNEETEKLPKP 350
BLAST of SMED30031708 vs. Planmine SMEST
Match: SMESG000059107.1 (SMESG000059107.1) HSP 1 Score: 165.622 bits (418), Expect = 1.805e-44 Identity = 109/198 (55.05%), Postives = 138/198 (69.70%), Query Frame = 1 Query: 1 DNQQQRINMMLDNGGVRXXXXXXXXXXXXXXXXXLQREEAREFQERWKSRDQNTRHINMMMVLNDEEIHTAEQCFLNSAGDKIDEHELNEKEDVIPTKRFK--TIELSSEEKIKRIQEFIKEKKSEKVTNKRNTKKVEEIQNEETQKIPKPKINEPPAKSRIQRILLQKIEDKI-REDENLTDEQIKEKINDRWFIVK 585 NQQQRINM+ +N VR+ N NQ QQ+ WQ LQ EEA+E Q RW+ R+ T+ INMM +N++EIHTAEQC +NS GD E++ EDV+P + K T +L+SEEKIKRIQEFIKEKK ++ K K+EEIQN+ET+ I KP K+RIQRILLQK+E+KI REDE LTDEQ+K+K+ RW K Sbjct: 1922 SNQQQRINMLQENEDVREQTINVNQGQQRAEWQRLQMEEAKEIQARWERRNGTTKRINMMEAVNEDEIHTAEQCVVNSDGD-----EMSVNEDVLPENKIKCETSKLTSEEKIKRIQEFIKEKKEKRREIKEIQMKIEEIQNDETKTITKPSKEAMATKNRIQRILLQKLEEKIRREDETLTDEQVKQKLETRWATTK 2114 HSP 2 Score: 80.8777 bits (198), Expect = 4.602e-16 Identity = 38/71 (53.52%), Postives = 51/71 (71.83%), Query Frame = 3 Query: 951 WAQRQPIKTIEAETDINNAQQLLNTEASTTDEWSNWIVDKGATTSRVPIIKLSSRKEVGTTTESRSRGRQK 1163 WAQ++P++T EAETD+ N +Q ++ + TTDEWSNWI D+GATTSR + +RKE GT +S RGR K Sbjct: 2860 WAQKEPVETTEAETDMENVRQAISKDMPTTDEWSNWIADRGATTSRELANRQPNRKEAGTMMDSNFRGRPK 2930 HSP 3 Score: 70.8626 bits (172), Expect = 8.137e-13 Identity = 35/67 (52.24%), Postives = 45/67 (67.16%), Query Frame = 3 Query: 747 RQMEYAEEIPEKYKLQIQYPDFENEEKEVHIATEIKDMKTSMPYEISFHLQYEGRSLDDEIEIGDEP 947 R +E A+ I + Y E EE+E H+ T+IK++K MPYE+ FHLQYEGRSLDDE+EIG P Sbjct: 2703 RAVELAKGIIDSY--------IETEEREEHVTTKIKEIKAKMPYELPFHLQYEGRSLDDELEIGVTP 2761
BLAST of SMED30031708 vs. Planmine SMEST
Match: SMESG000007496.1 (SMESG000007496.1) HSP 1 Score: 145.591 bits (366), Expect = 1.389e-37 Identity = 66/97 (68.04%), Postives = 83/97 (85.57%), Query Frame = 3 Query: 657 SLRTLVKEIEEYRRVYTSYPEQLEGMIRKWRQMEYAEEIPEKYKLQIQYPDFENEEKEVHIATEIKDMKTSMPYEISFHLQYEGRSLDDEIEIGDEP 947 +LRTLVKEIEEYRR+Y+ YPEQ +G+IRKW+ ME+AEE PEKY+L+IQYPD E EE E ++ T+IK++K +MPYE+ FHLQYE RSLDDE+EIG P Sbjct: 1578 NLRTLVKEIEEYRRIYSWYPEQFDGLIRKWKHMEWAEERPEKYRLKIQYPDIETEEGEEYVTTKIKEIKANMPYELHFHLQYERRSLDDELEIGVTP 1674 HSP 2 Score: 122.865 bits (307), Expect = 5.789e-30 Identity = 90/195 (46.15%), Postives = 116/195 (59.49%), Query Frame = 1 Query: 4 NQQQRINMMLDNGGVRXXXXXXXXXXXXXXXXXLQREEAREFQERWKSRDQNTRHINMMMVLNDEEIHTAEQCFLNSAGDKIDEHELNEKEDVIPTKRFKTIELSSEEKIKRIQEFIKEKKSEKVTNKRNTKKVEEIQNEETQKIPKPKINEPPAKSRIQRILLQKIEDKI-REDENLTDEQIKEKINDRWFIVK 585 NQQQRINM+ +NG VR+ N NQEQQ+ WQ LQ EEA+E Q RW+ R+ T+ INMM +N+ E+HTA+QC +NS GD+ +E +K +IK IQ K+EEIQN+ET+ I KP K+RIQRILLQK+E+KI REDE LTDEQ+K+K+ RW K Sbjct: 869 NQQQRINMLQENGDVREQIINVNQEQQRAEWQRLQMEEAKEIQARWERRNGTTKRINMMRAVNENEMHTADQCVVNSDGDETSVNEDRKKR---------------RREIKEIQ-----------------MKIEEIQNDETKTITKPSKKAMATKNRIQRILLQKLEEKIRREDETLTDEQVKQKLEKRWATAK 1031 HSP 3 Score: 88.9669 bits (219), Expect = 1.090e-18 Identity = 41/71 (57.75%), Postives = 53/71 (74.65%), Query Frame = 3 Query: 951 WAQRQPIKTIEAETDINNAQQLLNTEASTTDEWSNWIVDKGATTSRVPIIKLSSRKEVGTTTESRSRGRQK 1163 WAQR+P++T EAETD+ N +Q ++ + TTDEWSNWI D+GATTSR + RKE GT +S+SRGRQK Sbjct: 1745 WAQRKPVETTEAETDMENVRQAISKDMPTTDEWSNWIADRGATTSRELANRQPDRKEAGTMMDSKSRGRQK 1815
BLAST of SMED30031708 vs. Planmine SMEST
Match: SMESG000007496.1 (SMESG000007496.1) HSP 1 Score: 145.591 bits (366), Expect = 1.425e-37 Identity = 66/97 (68.04%), Postives = 83/97 (85.57%), Query Frame = 3 Query: 657 SLRTLVKEIEEYRRVYTSYPEQLEGMIRKWRQMEYAEEIPEKYKLQIQYPDFENEEKEVHIATEIKDMKTSMPYEISFHLQYEGRSLDDEIEIGDEP 947 +LRTLVKEIEEYRR+Y+ YPEQ +G+IRKW+ ME+AEE PEKY+L+IQYPD E EE E ++ T+IK++K +MPYE+ FHLQYE RSLDDE+EIG P Sbjct: 1604 NLRTLVKEIEEYRRIYSWYPEQFDGLIRKWKHMEWAEERPEKYRLKIQYPDIETEEGEEYVTTKIKEIKANMPYELHFHLQYERRSLDDELEIGVTP 1700 HSP 2 Score: 121.709 bits (304), Expect = 1.609e-29 Identity = 88/195 (45.13%), Postives = 116/195 (59.49%), Query Frame = 1 Query: 4 NQQQRINMMLDNGGVRXXXXXXXXXXXXXXXXXLQREEAREFQERWKSRDQNTRHINMMMVLNDEEIHTAEQCFLNSAGDKIDEHELNEKEDVIPTKRFKTIELSSEEKIKRIQEFIKEKKSEKVTNKRNTKKVEEIQNEETQKIPKPKINEPPAKSRIQRILLQKIEDKI-REDENLTDEQIKEKINDRWFIVK 585 NQQQRINM+ +NG VR+ N NQEQQ+ WQ LQ EEA+E Q RW+ R+ T+ INMM +N+ E+HTA+QC +NS GD+ +E N + + K I++ K+EEIQN+ET+ I KP K+RIQRILLQK+E+KI REDE LTDEQ+K+K+ RW K Sbjct: 869 NQQQRINMLQENGDVREQIINVNQEQQRAEWQRLQMEEAKEIQARWERRNGTTKRINMMRAVNENEMHTADQCVVNSDGDETSVNEDNSSKRKKRRREIKEIQM----------------------------KIEEIQNDETKTITKPSKKAMATKNRIQRILLQKLEEKIRREDETLTDEQVKQKLEKRWATAK 1035 HSP 3 Score: 88.9669 bits (219), Expect = 1.067e-18 Identity = 41/71 (57.75%), Postives = 53/71 (74.65%), Query Frame = 3 Query: 951 WAQRQPIKTIEAETDINNAQQLLNTEASTTDEWSNWIVDKGATTSRVPIIKLSSRKEVGTTTESRSRGRQK 1163 WAQR+P++T EAETD+ N +Q ++ + TTDEWSNWI D+GATTSR + RKE GT +S+SRGRQK Sbjct: 1771 WAQRKPVETTEAETDMENVRQAISKDMPTTDEWSNWIADRGATTSRELANRQPDRKEAGTMMDSKSRGRQK 1841
BLAST of SMED30031708 vs. Planmine SMEST
Match: SMESG000007496.1 (SMESG000007496.1) HSP 1 Score: 145.591 bits (366), Expect = 1.501e-37 Identity = 66/97 (68.04%), Postives = 83/97 (85.57%), Query Frame = 3 Query: 657 SLRTLVKEIEEYRRVYTSYPEQLEGMIRKWRQMEYAEEIPEKYKLQIQYPDFENEEKEVHIATEIKDMKTSMPYEISFHLQYEGRSLDDEIEIGDEP 947 +LRTLVKEIEEYRR+Y+ YPEQ +G+IRKW+ ME+AEE PEKY+L+IQYPD E EE E ++ T+IK++K +MPYE+ FHLQYE RSLDDE+EIG P Sbjct: 1458 NLRTLVKEIEEYRRIYSWYPEQFDGLIRKWKHMEWAEERPEKYRLKIQYPDIETEEGEEYVTTKIKEIKANMPYELHFHLQYERRSLDDELEIGVTP 1554 HSP 2 Score: 120.168 bits (300), Expect = 4.669e-29 Identity = 89/196 (45.41%), Postives = 113/196 (57.65%), Query Frame = 1 Query: 4 NQQQRINMMLDNGGVRXXXXXXXXXXXXXXXXXLQREEAREFQERWKSRDQNTRHINMMMVLNDEEIHTAEQCFLNSAGDKIDEHELNEKEDVIPTKRFKTIELSSEEKIKRIQEFIKEKKSEKVTNKRNTKKVE-EIQNEETQKIPKPKINEPPAKSRIQRILLQKIEDKI-REDENLTDEQIKEKINDRWFIVK 585 NQQQRINM+ +NG VR+ N NQEQQ+ WQ LQ EEA+E Q RW+ R+ T+ INMM +N+ E+HTA+QC +NS GD E + EDV+P N K E +IQN+ET+ I KP K+RIQRILLQK+E+KI REDE LTDEQ+K+K+ RW K Sbjct: 869 NQQQRINMLQENGDVREQIINVNQEQQRAEWQRLQMEEAKEIQARWERRNGTTKRINMMRAVNENEMHTADQCVVNSDGD-----ETSVNEDVLP---------------------------------ENIIKCETKIQNDETKTITKPSKKAMATKNRIQRILLQKLEEKIRREDETLTDEQVKQKLEKRWATAK 1026 HSP 3 Score: 88.9669 bits (219), Expect = 1.063e-18 Identity = 41/71 (57.75%), Postives = 53/71 (74.65%), Query Frame = 3 Query: 951 WAQRQPIKTIEAETDINNAQQLLNTEASTTDEWSNWIVDKGATTSRVPIIKLSSRKEVGTTTESRSRGRQK 1163 WAQR+P++T EAETD+ N +Q ++ + TTDEWSNWI D+GATTSR + RKE GT +S+SRGRQK Sbjct: 1625 WAQRKPVETTEAETDMENVRQAISKDMPTTDEWSNWIADRGATTSRELANRQPDRKEAGTMMDSKSRGRQK 1695 The following BLAST results are available for this feature:
BLAST of SMED30031708 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30031708 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30031708 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30031708 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30031708 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30031708 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30031708 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30031708 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30031708 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30031708 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30031708 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30031708 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30031708 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30031708 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30031708 ID=SMED30031708|Name=SMED30031708|organism=Schmidtea mediterranea sexual|type=transcript|length=1194bpback to top protein sequence of SMED30031708-orf-1 >SMED30031708-orf-1 ID=SMED30031708-orf-1|Name=SMED30031708-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=145bp MMMVLNDEEIHTAEQCFLNSAGDKIDEHELNEKEDVIPTKRFKTIELSSEback to top protein sequence of SMED30031708-orf-2 >SMED30031708-orf-2 ID=SMED30031708-orf-2|Name=SMED30031708-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=146bp MIRKWRQMEYAEEIPEKYKLQIQYPDFENEEKEVHIATEIKDMKTSMPYEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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