SMED30031695
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30031695 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Alignments
SMED30031695 aligns in the following genomic locations:
Homology
BLAST of SMED30031695 vs. Planmine SMEST
Match: SMESG000009161.1 (SMESG000009161.1) HSP 1 Score: 455.677 bits (1171), Expect = 1.182e-159 Identity = 236/270 (87.41%), Postives = 240/270 (88.89%), Query Frame = 3 Query: 117 QSNTQLIFSKLIKSFNIIDMKEVQPKPISNSFKQNQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTTKSES----------------------RNTSSDVDNSLQSNTQFSVSSLIKVFNNDDIKEVQLKPISNSFKQNQSTSVNINNISCDVDNTLQSNTQLSATKKTILSDSRPSFLQSPPAMISTKTTIKSSENSLTNPSTTRSTGDIIETSHDLQMAEKMVTEHSKKRKEEYSINYQLRPVQRLQNYKMYFLKFKHKSCCMIQ 860 QS TQ S LIK FNI+DMKEVQPKPISNSFK+NQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTTKSES NTSSDVDNSLQSNTQFSVSSLIKVFNNDDIKEVQLKPI NSFKQNQSTSVNINNISCDVDNTLQSNTQLSATKKTILSDSRPSFLQSPPAMISTKTTIKSSENSLTNPSTTRSTGDIIETSHDLQMAEKMVTEHSKKRKEEYSINYQLRPVQ+LQNYKMYFLKFKHKSCC+IQ Sbjct: 119 QSQTQFTISSLIKRFNIVDMKEVQPKPISNSFKKNQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTTKSESCNIITHSSFERPVPPKRKSININNTSSDVDNSLQSNTQFSVSSLIKVFNNDDIKEVQLKPICNSFKQNQSTSVNINNISCDVDNTLQSNTQLSATKKTILSDSRPSFLQSPPAMISTKTTIKSSENSLTNPSTTRSTGDIIETSHDLQMAEKMVTEHSKKRKEEYSINYQLRPVQKLQNYKMYFLKFKHKSCCLIQ 388
BLAST of SMED30031695 vs. Planmine SMEST
Match: SMESG000009116.1 (SMESG000009116.1) HSP 1 Score: 457.603 bits (1176), Expect = 4.270e-159 Identity = 237/270 (87.78%), Postives = 240/270 (88.89%), Query Frame = 3 Query: 117 QSNTQLIFSKLIKSFNIIDMKEVQPKPISNSFKQNQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTTKSES----------------------RNTSSDVDNSLQSNTQFSVSSLIKVFNNDDIKEVQLKPISNSFKQNQSTSVNINNISCDVDNTLQSNTQLSATKKTILSDSRPSFLQSPPAMISTKTTIKSSENSLTNPSTTRSTGDIIETSHDLQMAEKMVTEHSKKRKEEYSINYQLRPVQRLQNYKMYFLKFKHKSCCMIQ 860 QS TQ S LIK FNI+DMKEVQPKPISNSFKQNQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTTKSES NTSSDVDNSLQSNTQFSVSSLIKVFNNDDIKEVQLKPI NSFKQNQSTSVNINNISCDVDNTLQSNTQLSATKKTILSDSRPSFLQSPPAMISTKTTIKSSENSLTNPSTTRSTGDIIETSHDLQMAEKMVTEHSKKRKEEYSINYQLRPVQ+LQNYKMYFLKFKHKSCC+IQ Sbjct: 207 QSQTQFTISSLIKRFNIVDMKEVQPKPISNSFKQNQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTTKSESCNIITHSSFERPVPPKRKSININNTSSDVDNSLQSNTQFSVSSLIKVFNNDDIKEVQLKPICNSFKQNQSTSVNINNISCDVDNTLQSNTQLSATKKTILSDSRPSFLQSPPAMISTKTTIKSSENSLTNPSTTRSTGDIIETSHDLQMAEKMVTEHSKKRKEEYSINYQLRPVQKLQNYKMYFLKFKHKSCCLIQ 476 HSP 2 Score: 154.451 bits (389), Expect = 5.363e-42 Identity = 94/140 (67.14%), Postives = 102/140 (72.86%), Query Frame = 3 Query: 159 FNIIDM-----KEVQPKPISNSFKQNQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTTKSES----------------------RNTSSDVDNSLQSNTQFSVSSLIKVFNNDDIKEVQLKPISNSFKQNQSTSVNI 497 F+I+D+ KE Q KPISNSFKQNQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTTKSES N+SSDVDNSLQS TQF++SSLIK FN D+KEVQ KPISNSFKQNQ +S I Sbjct: 2 FDIVDINNDYIKEEQSKPISNSFKQNQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTTKSESCNIITHSSFERPVPQKRKSININNSSSDVDNSLQSQTQFTISSLIKRFNIVDMKEVQPKPISNSFKQNQLSSQTI 141
BLAST of SMED30031695 vs. Planmine SMEST
Match: SMESG000008713.1 (SMESG000008713.1) HSP 1 Score: 381.333 bits (978), Expect = 5.373e-127 Identity = 237/419 (56.56%), Postives = 266/419 (63.48%), Query Frame = 3 Query: 117 QSNTQLIFSKLIKSFNIIDMKEVQPKPISNSFKQNQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTTKSES----------------------RNTSSDVDNSLQSNTQFSVSSLIKVFNNDDIKEVQLKPISNSFK--------------------------------QNQST----------------------SVNINNISCDVDNTLQSNTQLSATKKTILSDSRPSFLQSPPAMISTKTTIKSSENSLTNPSTTRSTGDIIETSHDLQMAEKMVTEHSKKRKEEYSINYQLRPVQRLQNYKMYFLK--FKHKSCCMIQ*S*ENXXXXXXXXXXXXTMWM---FRKMNHH-------------ARFYNALQEITF*DN*SW*ITLNI*RFKVY*NDIVSLIWRIKRARFARE 1091 QSNTQ S LIK FNI+DMKEVQPKPISNSFKQNQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTTKSES N +SDVDNSLQSNTQF++SSLIK FN D+KEVQ KPISNSFK QN +T S+NINN + DVDN+LQSNT+ SA KKTILSDSRPSFLQSPPAMISTKTTIKSS++SLTNPSTTRSTGDIIE SHD +MA+KM+T +K+KEEYSINYQLRPVQ L+NYKMYFLK F+ K ++ S + I LS + F K+N H ++Y+ALQEI F DN SW IT+ I FKV NDIVS I RI+ AR A E Sbjct: 221 QSNTQFTISSLIKRFNIVDMKEVQPKPISNSFKQNQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTTKSESCNIITHSSFERPVPKQRKSININNKASDVDNSLQSNTQFTISSLIKRFNIVDMKEVQPKPISNSFKQNQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTTKSESCNIITHSSFERPVPKQRKSININNKASDVDNSLQSNTKFSANKKTILSDSRPSFLQSPPAMISTKTTIKSSKHSLTNPSTTRSTGDIIEISHDFEMADKMITGKHEKKKEEYSINYQLRPVQMLKNYKMYFLKNQFEIKLSDPVKLSKTSYPHLIATLSKNDMDVLKNEFIKINSHIQVTTTSFFNDDQTKYYDALQEIIFRDNESWYITVTIQGFKVDQNDIVSPICRIESARLALE 639 HSP 2 Score: 189.119 bits (479), Expect = 1.292e-53 Identity = 122/221 (55.20%), Postives = 131/221 (59.28%), Query Frame = 3 Query: 117 QSNTQLIFSKLIKSFNIIDMKEVQPKPISNSFKQNQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTTKSES----------------------RNTSSDVDNSLQSNTQFSVSSLIKVFNNDDIKEVQLKPISNSFKQNQ---------------STSV---------------------------------------NINNISCDVDNTLQSNTQLS 551 QSNTQ S LIK FNI+DMKEVQPKPISNSFKQNQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTTKSES N +SDVDNSLQSNTQF++SSLIK FN D+KEVQ KPISNSFKQNQ ST+V NINN + DVDN+LQSNTQ + Sbjct: 114 QSNTQFTISSLIKRFNIVDMKEVQPKPISNSFKQNQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTTKSESCNIITHSSFERPVPKQRKSININNKASDVDNSLQSNTQFTISSLIKRFNIVDMKEVQPKPISNSFKQNQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTTKSESCNIITHSSFERPVPKQRKSININNKASDVDNSLQSNTQFT 334 HSP 3 Score: 180.644 bits (457), Expect = 1.869e-50 Identity = 117/213 (54.93%), Postives = 126/213 (59.15%), Query Frame = 3 Query: 141 SKLIKSFNIIDMKEVQPKPISNSFKQNQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTTKSES----------------------RNTSSDVDNSLQSNTQFSVSSLIKVFNNDDIKEVQLKPISNSFKQNQ---------------STSV---------------------------------------NINNISCDVDNTLQSNTQLS 551 S LIK FNI+DMKEVQPKPISNSFKQNQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTTKSES N +SDVDNSLQSNTQF++SSLIK FN D+KEVQ KPISNSFKQNQ ST+V NINN + DVDN+LQSNTQ + Sbjct: 15 SSLIKRFNIVDMKEVQPKPISNSFKQNQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTTKSESCNIITHSSFERPVPKQRKSININNKASDVDNSLQSNTQFTISSLIKRFNIVDMKEVQPKPISNSFKQNQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTTKSESCNIITHSSFERPVPKQRKSININNKASDVDNSLQSNTQFT 227 HSP 4 Score: 48.521 bits (114), Expect = 7.780e-6 Identity = 41/108 (37.96%), Postives = 47/108 (43.52%), Query Frame = 3 Query: 390 SVSSLIKVFNNDDIKEVQLKPISNSFKQNQ---------------STSV---------------------------------------NINNISCDVDNTLQSNTQLS 551 +VSSLIK FN D+KEVQ KPISNSFKQNQ ST+V NINN + DVDN+LQSNTQ + Sbjct: 13 TVSSLIKRFNIVDMKEVQPKPISNSFKQNQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTTKSESCNIITHSSFERPVPKQRKSININNKASDVDNSLQSNTQFT 120
BLAST of SMED30031695 vs. Planmine SMEST
Match: SMESG000050885.1 (SMESG000050885.1) HSP 1 Score: 132.109 bits (331), Expect = 1.036e-35 Identity = 91/166 (54.82%), Postives = 106/166 (63.86%), Query Frame = 3 Query: 141 SKLIKSFNIIDMKEVQPKPISNSFKQNQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTTKSESRNTSSDVDNSLQSNTQFSVSSLIKVFNNDDIKEVQLKPISNSFKQNQSTSVNINNISCDVDNTLQSNTQLSATKKTILSDSRPSFLQSPPAMISTKTTIK 638 S LIK FN +D+KE+QPK + NSFKQNQ +S TISKHTNQISSSTT+T N+H SSQYQNSTTKSES SS++ +S P S N+ + NNIS D D QSN QLSATKKTILSDSRPS Q+PPAMIS+K TI+ Sbjct: 15 SSLIKKFNNVDIKEIQPKTVCNSFKQNQFTSHTISKHTNQISSSTTITSNYHHSSQYQNSTTKSES---SSNIIHS------------------------SFGP---SLPHNRKINNI-NNISSDAD---QSNKQLSATKKTILSDSRPSISQNPPAMISSKRTIR 146 HSP 2 Score: 47.3654 bits (111), Expect = 8.328e-6 Identity = 25/35 (71.43%), Postives = 27/35 (77.14%), Query Frame = 3 Query: 393 VSSLIKVFNNDDIKEVQLKPISNSFKQNQSTSVNI 497 VSSLIK FNN DIKE+Q K + NSFKQNQ TS I Sbjct: 14 VSSLIKKFNNVDIKEIQPKTVCNSFKQNQFTSHTI 48
BLAST of SMED30031695 vs. Planmine SMEST
Match: SMESG000007863.1 (SMESG000007863.1) HSP 1 Score: 41.2022 bits (95), Expect = 1.068e-8 Identity = 25/51 (49.02%), Postives = 33/51 (64.71%), Query Frame = 3 Query: 942 HARFYNALQEITF*DN*SW*ITLNI*RFKVY*NDIVSLIWRIKRARFAREF 1094 ARFY+A Q+I F +N + +T+ I RFKV N+ IWR+KRAR A F Sbjct: 61 QARFYDAFQKIMFKENENSYVTIKIQRFKVDQNN----IWRLKRARLALGF 107 HSP 2 Score: 37.3502 bits (85), Expect = 1.068e-8 Identity = 19/31 (61.29%), Postives = 21/31 (67.74%), Query Frame = 2 Query: 839 QIMLYDPVKLRKLPYPHLMTILSNNDVDV*K 931 +I L +PVKL K PY HLM LS DVDV K Sbjct: 9 EIKLTNPVKLCKTPYRHLMATLSTEDVDVLK 39 The following BLAST results are available for this feature:
BLAST of SMED30031695 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30031695 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30031695 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30031695 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30031695 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30031695 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30031695 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30031695 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30031695 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30031695 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30031695 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30031695 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30031695 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30031695 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30031695 ID=SMED30031695|Name=SMED30031695|organism=Schmidtea mediterranea sexual|type=transcript|length=1258bpback to top protein sequence of SMED30031695-orf-1 >SMED30031695-orf-1 ID=SMED30031695-orf-1|Name=SMED30031695-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=230bp MKEVQPKPISNSFKQNQLSSQTISKHTNQISSSTTVTPNHHDSSQYQNSTback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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