GCR009

Overview
NameGCR009
Smed IDSMED30031677
Length (bp)1434
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of GCR009 (SMED30031677) t-SNE clustered cells

Violin plots show distribution of expression levels for GCR009 (SMED30031677) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of GCR009 (SMED30031677) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for GCR009 (SMED30031677) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30031677

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30031677h1SMcG0021107 SmedASXL_005923SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30031677h1SMcG0021107 SmedASXL_005924SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
cilated neuronSMED30031677h1SMcG0021107 dd_Smed_v4_15625_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
anterior sideSMED30031677h1SMcG0021107 dd_Smed_v4_15625_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30031677h1SMcG0021107 dd_Smed_v6_15625_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of GCR009 vs. Ensembl Human
Match: TACR3 (tachykinin receptor 3 [Source:HGNC Symbol;Acc:HGNC:11528])

HSP 1 Score: 163.31 bits (412), Expect = 2.213e-44
Identity = 104/357 (29.13%), Postives = 172/357 (48.18%), Query Frame = 1 
Query:  217 FGNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWNNNNLQ-ISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMND----SFKSDFRWLVYHKCCGLCQRQSWSRKINFNTTSARNSVSNNFSPALTIQNRKYNDKFPSRDHSSPERSKEIQFLFNKDNNECVNHNS 1272
             GN++VI   W+             FL NLA SD  ++ F     F Y L  +W F   +C       + AV AS  +   I +DR+  +I P+K R++   + +VIG IW+ + L +  QC  S+  +  G  T C   W     Q  +Y I ++++ Y  PL+++ +TYTIVGI LW   +PG+  + +  + +K+K KV+KM+ I+V+ F +CWLP H++F++T + Q     K +   Y+   W+AMS++  NP++Y  +N      FK  FRW  + K     + +  + + + N  S+  +V+   S  +       +    SR   +  R            N C   NS
Sbjct:  101 LGNLIVI---WIILAHKRMRTVTNYFLVNLAFSDASMAAFNTLVNFIYALHSEWYFGANYCRFQNFFPITAVFASIYSMTAIAVDRYMAIIDPLKPRLSATATKIVIGSIWILAFLLAFPQCLYSKTKVMPGR-TLCFVQWPEGPKQHFTYHIIVIILVYCFPLLIMGITYTIVGITLWGGEIPGDTCD-KYHEQLKAKRKVVKMMIIVVMTFAICWLPYHIYFILTAIYQQLNRWKYIQQVYLASFWLAMSSTMYNPIIYCCLNKRFRAGFKRAFRWCPFIKVSSYDELELKTTRFHPNRQSSMYTVTRMESMTVVFDPNDADTTRSSRKKRATPRDPSF--------NGCSRRNS 444
[Close]
BLAST of GCR009 vs. Ensembl Human
Match: TACR2 (tachykinin receptor 2 [Source:HGNC Symbol;Acc:HGNC:11527])

HSP 1 Score: 158.303 bits (399), Expect = 4.502e-43
Identity = 82/282 (29.08%), Postives = 148/282 (52.48%), Query Frame = 1 
Query:  220 GNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENW---NNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCC 1056
            GN +VI   W+             F+ NLA++DL ++ F   F F Y     W F   FC       + A+  S  +   I  DR+  +++P + R++   +  VI  IW+ ++  +S QCF S  ++ +G  T+C   W   +     + Y + ++ + YFLPL V+ V Y+++G+ LW+R++PG      + +H+++  K +K + ++V+ F +CWLP HL+F++    +     K +   Y+   W+AMS++  NP++Y  +N  F+S FR  +  +CC
Sbjct:   50 GNAIVI---WIILAHRRMRTVTNYFIVNLALADLCMAAFNAAFNFVYASHNIWYFGRAFCYFQNLFPITAMFVSIYSMTAIAADRYMAIVHPFQPRLSAPSTKAVIAGIWLVALALASPQCFYSTVTMDQGA-TKCVVAWPEDSGGKTLLLYHLVVIALIYFLPLAVMFVAYSVIGLTLWRRAVPGHQAHGANLRHLQAMKKFVKTMVLVVLTFAICWLPYHLYFILGSFQEDIYCHKFIQQVYLALFWLAMSSTMYNPIIYCCLNHRFRSGFR--LAFRCC 325
[Close]
BLAST of GCR009 vs. Ensembl Human
Match: TACR2 (tachykinin receptor 2 [Source:HGNC Symbol;Acc:HGNC:11527])

HSP 1 Score: 155.992 bits (393), Expect = 1.531e-42
Identity = 77/258 (29.84%), Postives = 141/258 (54.65%), Query Frame = 1 
Query:  292 FLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENW---NNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCC 1056
            F+ NLA++DL ++ F   F F Y     W F   FC       + A+  S  +   I  DR+  +++P + R++   +  VI  IW+ ++  +S QCF S  ++ +G  T+C   W   +     + Y + ++ + YFLPL V+ V Y+++G+ LW+R++PG      + +H+++  K +K + ++V+ F +CWLP HL+F++    +     K +   Y+   W+AMS++  NP++Y  +N  F+S FR  +  +CC
Sbjct:   35 FIVNLALADLCMAAFNAAFNFVYASHNIWYFGRAFCYFQNLFPITAMFVSIYSMTAIAADRYMAIVHPFQPRLSAPSTKAVIAGIWLVALALASPQCFYSTVTMDQGA-TKCVVAWPEDSGGKTLLLYHLVVIALIYFLPLAVMFVAYSVIGLTLWRRAVPGHQAHGANLRHLQAMKKFVKTMVLVVLTFAICWLPYHLYFILGSFQEDIYCHKFIQQVYLALFWLAMSSTMYNPIIYCCLNHRFRSGFR--LAFRCC 289
[Close]
BLAST of GCR009 vs. Ensembl Human
Match: NPY2R (neuropeptide Y receptor Y2 [Source:HGNC Symbol;Acc:HGNC:7957])

HSP 1 Score: 143.665 bits (361), Expect = 7.064e-38
Identity = 90/253 (35.57%), Postives = 142/253 (56.13%), Query Frame = 1 
Query:  292 FLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIW-VSSILFSSVQCFVSRASIR---EGGYTECSENWNNNNLQI---SYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDF 1029
            F+ NLAV+DLL++  C+PFT TY L+ +W    + C +V   Q LAV  S+IT  VI LDR   ++Y ++S+I+++ S L+IG  W +S++L S +  F   + I    +     C+E W      I    Y++S LLI Y LPL +++ +YT +  KL     PG A+   D  H + + K  KML  +V+ F + WLPLH F +   ++      K   + + V H IAM ++F NP++Y +MN +++  F
Sbjct:   88 FIANLAVADLLVNTLCLPFTLTYTLMGEWKMGPVLCHLVPYAQGLAVQVSTITLTVIALDRHRCIVYHLESKISKRISFLIIGLAWGISALLASPLAIFREYSLIEIIPDFEIVACTEKWPGEEKSIYGTVYSLSSLLILYVLPLGIISFSYTRIWSKLKNHVSPGAAN---DHYHQRRQ-KTTKMLVCVVVVFAVSWLPLHAFQLAVDIDSQVLDLKEYKLIFTVFHIIAMCSTFANPLLYGWMNSNYRKAF 336
[Close]
BLAST of GCR009 vs. Ensembl Human
Match: NPY2R (neuropeptide Y receptor Y2 [Source:HGNC Symbol;Acc:HGNC:7957])

HSP 1 Score: 143.665 bits (361), Expect = 7.064e-38
Identity = 90/253 (35.57%), Postives = 142/253 (56.13%), Query Frame = 1 
Query:  292 FLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIW-VSSILFSSVQCFVSRASIR---EGGYTECSENWNNNNLQI---SYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDF 1029
            F+ NLAV+DLL++  C+PFT TY L+ +W    + C +V   Q LAV  S+IT  VI LDR   ++Y ++S+I+++ S L+IG  W +S++L S +  F   + I    +     C+E W      I    Y++S LLI Y LPL +++ +YT +  KL     PG A+   D  H + + K  KML  +V+ F + WLPLH F +   ++      K   + + V H IAM ++F NP++Y +MN +++  F
Sbjct:   88 FIANLAVADLLVNTLCLPFTLTYTLMGEWKMGPVLCHLVPYAQGLAVQVSTITLTVIALDRHRCIVYHLESKISKRISFLIIGLAWGISALLASPLAIFREYSLIEIIPDFEIVACTEKWPGEEKSIYGTVYSLSSLLILYVLPLGIISFSYTRIWSKLKNHVSPGAAN---DHYHQRRQ-KTTKMLVCVVVVFAVSWLPLHAFQLAVDIDSQVLDLKEYKLIFTVFHIIAMCSTFANPLLYGWMNSNYRKAF 336
[Close]
BLAST of GCR009 vs. Ensembl Celegans
Match: npr-22 (Neuropeptide receptor 22 [Source:UniProtKB/Swiss-Prot;Acc:Q59E83])

HSP 1 Score: 140.198 bits (352), Expect = 1.101e-36
Identity = 94/291 (32.30%), Postives = 156/291 (53.61%), Query Frame = 1 
Query:  217 FGNVVVIIVF-WLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWD---WPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKS--RITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENW-----NNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPG---EADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLN----IAYV--VCHWIAMSNSFVNPVVYSFMNDSFKSDF 1029
            +GNV+VI+V  +    R   N      + NLAV+DLLIS+FC+PF++  +L++D   W F  + C ++  +Q +AV  S+ T +VI  DR+  +++ +    RITR+R+  ++   W+ SIL +    F +R    + G   C ENW     +   ++  Y+  +L++ Y +P  VL +TYT +GIK+W   +PG    A +       +S  K++ M+ +I   F LCWLPL +      +N    +  ++N    I Y+    H +AMS+S VNP++Y   N  F+  F
Sbjct:   66 YGNVLVILVIVYFKRLRTATN----ILILNLAVADLLISVFCIPFSYWQVLIYDDQRWLFGSMMCSLLAFLQAMAVFLSAWTLVVISFDRWMAIMFLLTPNIRITRRRALYLVAATWIFSILMALPLLFTTRFFEDQDGLPNCGENWTYFGDSGEQVRKVYSSMVLILQYVVPQAVLIITYTHIGIKMWNSRVPGMQNGATKKMIVDRHESVKKLVPMVILISALFALCWLPLLIL-----INVIPEFYPDINSWGYILYLWWFAHGLAMSHSMVNPIIYFIRNARFREGF 347
[Close]
BLAST of GCR009 vs. Ensembl Celegans
Match: npr-22 (Neuropeptide receptor 22 [Source:UniProtKB/Swiss-Prot;Acc:Q59E83])

HSP 1 Score: 140.584 bits (353), Expect = 1.118e-36
Identity = 94/291 (32.30%), Postives = 156/291 (53.61%), Query Frame = 1 
Query:  217 FGNVVVIIVF-WLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWD---WPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKS--RITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENW-----NNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPG---EADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLN----IAYV--VCHWIAMSNSFVNPVVYSFMNDSFKSDF 1029
            +GNV+VI+V  +    R   N      + NLAV+DLLIS+FC+PF++  +L++D   W F  + C ++  +Q +AV  S+ T +VI  DR+  +++ +    RITR+R+  ++   W+ SIL +    F +R    + G   C ENW     +   ++  Y+  +L++ Y +P  VL +TYT +GIK+W   +PG    A +       +S  K++ M+ +I   F LCWLPL +      +N    +  ++N    I Y+    H +AMS+S VNP++Y   N  F+  F
Sbjct:   66 YGNVLVILVIVYFKRLRTATN----ILILNLAVADLLISVFCIPFSYWQVLIYDDQRWLFGSMMCSLLAFLQAMAVFLSAWTLVVISFDRWMAIMFLLTPNIRITRRRALYLVAATWIFSILMALPLLFTTRFFEDQDGLPNCGENWTYFGDSGEQVRKVYSSMVLILQYVVPQAVLIITYTHIGIKMWNSRVPGMQNGATKKMIVDRHESVKKLVPMVILISALFALCWLPLLIL-----INVIPEFYPDINSWGYILYLWWFAHGLAMSHSMVNPIIYFIRNARFREGF 347
[Close]
BLAST of GCR009 vs. Ensembl Celegans
Match: tkr-3 (TachyKinin Receptor family [Source:UniProtKB/TrEMBL;Acc:Q58AU0])

HSP 1 Score: 125.561 bits (314), Expect = 1.294e-31
Identity = 77/295 (26.10%), Postives = 141/295 (47.80%), Query Frame = 1 
Query:  235 IIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWNNNNLQI--------------SYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPG-EADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCCGLCQRQ 1074
            ++V ++   R    +    F+ NL  +DL   +F +PF F   L  +W  P   C +V  ++ +A+T S  T +   +  F  + +   S+++ + +   +  IW+ ++L           S+  G +    E  ++NN  I              +Y + +++I YF+P+I+L   YT++ +K+W  S    E DE + A       K+++ L I+V CF LCW PL  + ++  +       K +N+ +   HW+AMSNS +NP++Y   N  +  ++R L     C + QRQ
Sbjct:   65 LLVLYITLFRLRVRSITTYFILNLGFADLFTGIFAIPFKFQAALFQEWFLPRSLCRIVPYVETVALTVSVFTLVTSAVHEFRTMFFSKCSQMSPRSAKRCVLLIWIMAVL----------VSLPHGLFHNTYEFPDDNNTSIVQCLPVYPDAGWWKTYNVYLVIIQYFVPMIILDTAYTMIAVKIWSLSQSRVELDETKMATQ-----KLMRTLIIVVACFSLCWFPLETYLLLNELKPEINGWKYINLVFFFSHWLAMSNSCLNPIIYGLYNTKYNEEYRRLFRQIGC-IWQRQ 343
[Close]
BLAST of GCR009 vs. Ensembl Celegans
Match: tkr-3 (TachyKinin Receptor family [Source:UniProtKB/TrEMBL;Acc:Q58AU0])

HSP 1 Score: 125.176 bits (313), Expect = 1.781e-31
Identity = 78/307 (25.41%), Postives = 143/307 (46.58%), Query Frame = 1 
Query:  235 IIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWNNNNLQI--------------SYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLS-------------IIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCCGLCQRQ 1074
            ++V ++   R    +    F+ NL  +DL   +F +PF F   L  +W  P   C +V  ++ +A+T S  T +   +  F  + +   S+++ + +   +  IW+ ++L           S+  G +    E  ++NN  I              +Y + +++I YF+P+I+L   YT++ +K+W  SL     E+ + K    K+ V+ M+S             I+V CF LCW PL  + ++  +       K +N+ +   HW+AMSNS +NP++Y   N  +  ++R L     C + QRQ
Sbjct:   65 LLVLYITLFRLRVRSITTYFILNLGFADLFTGIFAIPFKFQAALFQEWFLPRSLCRIVPYVETVALTVSVFTLVTSAVHEFRTMFFSKCSQMSPRSAKRCVLLIWIMAVL----------VSLPHGLFHNTYEFPDDNNTSIVQCLPVYPDAGWWKTYNVYLVIIQYFVPMIILDTAYTMIAVKIW--SLSQSRVELDETKMATQKISVVSMVSPNTQLSQLMRTLIIVVACFSLCWFPLETYLLLNELKPEINGWKYINLVFFFSHWLAMSNSCLNPIIYGLYNTKYNEEYRRLFRQIGC-IWQRQ 358
[Close]
BLAST of GCR009 vs. Ensembl Celegans
Match: tkr-2 (TachyKinin Receptor family [Source:UniProtKB/TrEMBL;Acc:O44148])

HSP 1 Score: 111.694 bits (278), Expect = 8.807e-27
Identity = 81/282 (28.72%), Postives = 143/282 (50.71%), Query Frame = 1 
Query:  241 VFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIR--EGGYTE------CSENWNNNN---LQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNI-AYVVCHWIAMSNSFVNPVVYSFMNDSFK----SDFRWL 1038
            V W+        +    +L NLAV+D  IS+F   F+++Y   + W F   +C +   + +  + AS  T +V+ ++R++ +I+P+K R  R+ +  +I  IW  + LF       S+  +     GYT        ++N+   N   L   Y   ++ + Y LPL +L+  Y  VG++L K    G   ++R AK + +K K   ML+++V  F + W P + +++  H+ +  G  K L++  Y+  +W+ MS++  NPV+Y FMN  F+      FRWL
Sbjct:   82 VMWIILYHRQMRSVTNYYLFNLAVADASISVFNTGFSWSYNYYYVWKFGSFYCRINNLMGITPICASVFTMIVMSIERYYAIIHPLKKRPGRRSTVTIIIMIWFMAFLFGVPAFLASKVDVYYFYDGYTLYENPLCLADNYPGGNESLLGQVYNNGLITVQYILPLCILSAAYYRVGVELRKDKTVG---DVRHAKSVAAKKKASIMLAVVVFIFMIVWFPYNAYYLTLHLVEPIG-NKMLSLYIYINIYWLGMSSTVFNPVIYYFMNKRFRVGFHHAFRWL 359
[Close]
BLAST of GCR009 vs. Ensembl Fly
Match: TkR99D (gene:FBgn0004622 transcript:FBtr0085507)

HSP 1 Score: 170.244 bits (430), Expect = 9.598e-47
Identity = 114/375 (30.40%), Postives = 194/375 (51.73%), Query Frame = 1 
Query:  217 FGNVVVI------IVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRAS---IRE-GGYTECSENW-----NNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCCGL-CQRQSWSRKINFNTTSARNSVSNNFSPALTIQNR-KYNDKFPSRDHSSPERSKEIQFLFNKDNNECVNHNSIDLPRE 1290
            FG +V++      IV W+             F+ NL+++D ++S   V F + Y+L  DWPF   +C +   I +L++ AS  T M I +DR+  +I P++ R++++ +  +   IW++S L S     + R     +R     T C   W     N++ ++  Y I I+++TYFLP++ +TVTY+ VGI+LW     GE    R  ++++SK +V+KM+ ++V+ F +CWLP H +F++T    +      +   Y+  +W+AMSNS  NP++Y +MN  F+  F+ +V+  C  +    + +SR+ N    ++R S S   SP     NR K ND   S  ++ P   K  +   +         NS  LP E
Sbjct:  108 FGGMVIVATGGNLIVVWIVMTTKRMRTVTNYFIVNLSIADAMVSSLNVTFNYYYMLDSDWPFGEFYCKLSQFIAMLSICASVFTLMAISIDRYVAIIRPLQPRMSKRCNLAIAAVIWLASTLISCPMMIIYRTEEVPVRGLSNRTVCYPEWPDGPTNHSTMESLYNILIIILTYFLPIVSMTVTYSRVGIELWGSKTIGECTP-RQVENVRSKRRVVKMMIVVVLIFAICWLPFHSYFIITSCYPAITEAPFIQELYLAIYWLAMSNSMYNPIIYCWMNSRFRYGFK-MVFRWCLFVRVGTEPFSRRENL---TSRYSCSG--SPD---HNRIKRNDTQKSILYTCPSSPKSHRISHSGTGRSATLRNS--LPAE 470
[Close]
BLAST of GCR009 vs. Ensembl Fly
Match: TkR99D (gene:FBgn0004622 transcript:FBtr0301656)

HSP 1 Score: 169.088 bits (427), Expect = 2.364e-46
Identity = 93/294 (31.63%), Postives = 163/294 (55.44%), Query Frame = 1 
Query:  217 FGNVVVI------IVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRAS---IRE-GGYTECSENW-----NNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKC 1053
            FG +V++      IV W+             F+ NL+++D ++S   V F + Y+L  DWPF   +C +   I +L++ AS  T M I +DR+  +I P++ R++++ +  +   IW++S L S     + R     +R     T C   W     N++ ++  Y I I+++TYFLP++ +TVTY+ VGI+LW     GE    R  ++++SK +V+KM+ ++V+ F +CWLP H +F++T    +      +   Y+  +W+AMSNS  NP++Y +MN  F+  F+ +V+  C
Sbjct:  108 FGGMVIVATGGNLIVVWIVMTTKRMRTVTNYFIVNLSIADAMVSSLNVTFNYYYMLDSDWPFGEFYCKLSQFIAMLSICASVFTLMAISIDRYVAIIRPLQPRMSKRCNLAIAAVIWLASTLISCPMMIIYRTEEVPVRGLSNRTVCYPEWPDGPTNHSTMESLYNILIIILTYFLPIVSMTVTYSRVGIELWGSKTIGECTP-RQVENVRSKRRVVKMMIVVVLIFAICWLPFHSYFIITSCYPAITEAPFIQELYLAIYWLAMSNSMYNPIIYCWMNSRFRYGFK-MVFRWC 399
[Close]
BLAST of GCR009 vs. Ensembl Fly
Match: TkR99D (gene:FBgn0004622 transcript:FBtr0330298)

HSP 1 Score: 169.859 bits (429), Expect = 2.597e-46
Identity = 114/375 (30.40%), Postives = 194/375 (51.73%), Query Frame = 1 
Query:  217 FGNVVVI------IVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRAS---IRE-GGYTECSENW-----NNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCCGL-CQRQSWSRKINFNTTSARNSVSNNFSPALTIQNR-KYNDKFPSRDHSSPERSKEIQFLFNKDNNECVNHNSIDLPRE 1290
            FG +V++      IV W+             F+ NL+++D ++S   V F + Y+L  DWPF   +C +   I +L++ AS  T M I +DR+  +I P++ R++++ +  +   IW++S L S     + R     +R     T C   W     N++ ++  Y I I+++TYFLP++ +TVTY+ VGI+LW     GE    R  ++++SK +V+KM+ ++V+ F +CWLP H +F++T    +      +   Y+  +W+AMSNS  NP++Y +MN  F+  F+ +V+  C  +    + +SR+ N    ++R S S   SP     NR K ND   S  ++ P   K  +   +         NS  LP E
Sbjct:  108 FGGMVIVATGGNLIVVWIVMTTKRMRTVTNYFIVNLSIADAMVSSLNVTFNYYYMLDSDWPFGEFYCKLSQFIAMLSICASVFTLMAISIDRYVAIIRPLQPRMSKRCNLAIAAVIWLASTLISCPMMIIYRTEEVPVRGLSNRTVCYPEWPDGPTNHSTMESLYNILIIILTYFLPIVSMTVTYSRVGIELWGSKTIGECTP-RQVENVRSKRRVVKMMIVVVLIFAICWLPFHSYFIITSCYPAITEAPFIQELYLAIYWLAMSNSMYNPIIYCWMNSRFRYGFK-MVFRWCLFVRVGTEPFSRRENL---TSRYSCSG--SPD---HNRIKRNDTQKSILYTCPSSPKSHRISHSGTGRSATLRNS--LPAE 470
[Close]
BLAST of GCR009 vs. Ensembl Fly
Match: Lkr (gene:FBgn0035610 transcript:FBtr0077149)

HSP 1 Score: 163.696 bits (413), Expect = 2.641e-44
Identity = 118/373 (31.64%), Postives = 179/373 (47.99%), Query Frame = 1 
Query:  220 GNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWN-------NNNLQ----ISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCCGL-----CQRQSWS---RKINFNTTSARNSV---SNNFSPAL-TIQNRKYNDKFPSRDHSSPERSKEIQFLFNKDNNECVNHN 1269
            GN +VI V  + T R  R      ++ NLA +D++I LFC+PF F   LL  W  P+  C     +Q L+V  S  T   I +DR   +I P+++R T+  S  +IG IW+ ++LF+       R       + E +E +N       N NL      S+  +++ + Y +P  V++  Y  + ++LW    PG A + RD   +K+K KVIKML I+VI FGLCWLPL L+ ++            ++I +  C W+AMSNS  NP +Y   N+ FK +F        C          +++S   R  +  +T A +S+   SN F PA   + N K     P R H S   S        ++NN    H+
Sbjct:   52 GNTLVIWV--VATTRQMRT-VTNMYIANLAFADVIIGLFCIPFQFQAALLQSWNLPWFMCSFCPFVQALSVNVSVFTLTAIAIDRHRAIINPLRARPTKFVSKFIIGGIWMLALLFAVPFAIAFRVEELTERFRENNETYNVTRPFCMNKNLSDDQLQSFRYTLVFVQYLVPFCVISFVYIQMAVRLWGTRAPGNAQDSRDITLLKNKKKVIKMLIIVVIIFGLCWLPLQLYNILYVTIPEINDYHFISIVWFCCDWLAMSNSCYNPFIYGIYNEKFKREFNKRFAACFCKFKTSMDAHERTFSMHTRASSIRSTYANSSMRIRSNLFGPARGGVNNGKPGLHMP-RVHGSGANSGIYNGSSGQNNNVNGQHH 420
[Close]
BLAST of GCR009 vs. Ensembl Fly
Match: TkR86C (gene:FBgn0004841 transcript:FBtr0082315)

HSP 1 Score: 160.614 bits (405), Expect = 2.242e-43
Identity = 111/403 (27.54%), Postives = 199/403 (49.38%), Query Frame = 1 
Query:  238 IVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGY-----TECSENWNNNNLQIS-----YTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCCGLCQRQ--SWSRKINFNTTSARNSVSNNFS----PALTIQNRKYNDKFPSRDHSSPERSKEIQFLFNKDNNECVNHNSIDLPREFQVKSKPLSTNMN-------KIKILILDAEVEKNRI 1377
            IV W+ T   +       FL NL+++DLL+S     F F ++L  DWPF  ++C +   +  + V+ S  T + I  DR+  +++P+K R +R++  +++  IW  S + S+  C +  + + +  Y     T C   W +     S     Y + IL++TY +P+IV+ + Y+++G  LW     GE  + R  + MKSK KV++M   IV  F +CWLP HLFF+  + N     TK +   Y+  +W+AMSN+ VNP++Y +MN  F+  F+ ++   C GL + +  S   ++    +S R++ +   S      +  Q ++       R+  S ++ +      N+   EC+      + R     S PL  ++N       ++KI  +  + + N +
Sbjct:  103 IVLWIVTGHRSMRTVTNYFLLNLSIADLLMSSLNCVFNFIFMLNSDWPFGSIYCTINNFVANVTVSTSVFTLVAISFDRYIAIVHPLKRRTSRRKVRIILVLIWALSCVLSA-PCLLYSSIMTKHYYNGKSRTVCFMMWPDGRYPTSMADYAYNLIILVLTYGIPMIVMLICYSLMGRVLWGSRSIGENTD-RQMESMKSKRKVVRMFIAIVSIFAICWLPYHLFFIYAYHNNQVASTKYVQHMYLGFYWLAMSNAMVNPLIYYWMNKRFRMYFQRIICCCCVGLTRHRFDSPKSRLTNKNSSNRHTRAETKSQWKRSTMETQIQQAPVTSSCREQRSAQQQQPPGSGTNRAAVECI------MERPADGSSSPLCLSINNSIGERQRVKIKYISCDEDNNPV 497
[Close]
BLAST of GCR009 vs. Ensembl Zebrafish
Match: npffr1l2 (neuropeptide FF receptor 1 like 2 [Source:ZFIN;Acc:ZDB-GENE-060526-110])

HSP 1 Score: 158.303 bits (399), Expect = 1.704e-42
Identity = 111/332 (33.43%), Postives = 162/332 (48.80%), Query Frame = 1 
Query:  292 FLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILF---SSVQCFVSRAS----IREGGYTE------CSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSL------------------PGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYV--VCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLV-YHKCC--GLCQRQSWSRKINFNTTS-ARNSVSNNFSPALTIQNRKYND 1176
            F+ NLAVSDLL+ +FC+P T    L+  WPF    C +   +Q ++V+AS  T + I +DRF  ++YP + ++T   + + I  IWV +++    S+V   V R      +R   Y        C ENW N  ++  YT  +    Y +PL ++T+ Y  +GIKL+  S+                  PG   E R     K K+KVIKMLSI+ + F + WLPL    ++T           L   YV    HW+A SNS VNP++Y + N++FK  F+ +   H CC  G+  R+   RK    T   A NS   NF      +NR Y D
Sbjct:   90 FILNLAVSDLLVGVFCIPTTLVDNLITGWPFTNTVCKMSGLVQGMSVSASVFTLVAIAVDRFRCIVYPFQPKLTLLVAKVTIVMIWVLAVVIMCPSAVTLTVERVEHHYMVRGEDYNHTYPLFSCFENWANPQMRKVYTTVLFAHIYLIPLTLITLMYGRIGIKLYTTSVISGNDQHDGGQPHTSPQAPGGQQEGRPLISQK-KIKVIKMLSIVALLFTISWLPLWTLMLLTDYGGLNEDELELLSGYVFPFAHWLAFSNSSVNPIIYGYYNENFKRGFQAVCRAHSCCFEGMTARRGMRRKARGETRDPAVNSNPLNFGA----RNRVYTD 416
[Close]
BLAST of GCR009 vs. Ensembl Zebrafish
Match: CU915819.2 (neuropeptide FF receptor 2a [Source:NCBI gene;Acc:560955])

HSP 1 Score: 156.762 bits (395), Expect = 1.812e-42
Identity = 93/295 (31.53%), Postives = 154/295 (52.20%), Query Frame = 1 
Query:  292 FLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQC--------------FVSRASIREGGYTECSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKH--MKSKVKVIKMLSIIVICFGLCWLPL-HLFFVVTHVNQSQGYTKNLNI-AYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCCGL-CQRQSWSRKINFNTTSARN 1119
            F+ NLA+SDLL+ +FC+P T    ++  WPF  + C +   +Q ++V+AS  T + I +DRF  ++YP K ++T   +  +I  IWV ++   S+ C               V R + R   +  C ENW N  ++  YT  +    Y  PL ++ + Y  +GI L+K ++P       D +H   K K +VIKML I+ + F L WLPL  L  +  +V  ++   + +NI  Y   HW+A  NS VNP++Y F N++F+  F+ +     C +  Q +++S ++  N+    N
Sbjct:   84 FILNLAISDLLVGIFCMPTTLLDNIITGWPFGSMVCKMSGMVQGISVSASVFTLVAIAVDRFRCIVYPFKQKLTISTATFIIVIIWVLAV---SIMCPSGVMLQVTKEQNIRVFRGNNRSSPFYWCRENWPNQEMRKIYTTVLFANIYLAPLSLIVIMYARIGITLFKTAMPAGGKHGHDNRHSVSKKKQRVIKMLLIVALLFILSWLPLWTLMMLTDYVKLTEHQYRVINIYIYPFAHWLAFFNSSVNPIIYGFFNENFRRGFQAIFKFGLCPVGGQHRTYSHRVQGNSVQPVN 375
[Close]
BLAST of GCR009 vs. Ensembl Zebrafish
Match: AL929374.1 (neuropeptide FF receptor 2a [Source:NCBI gene;Acc:560955])

HSP 1 Score: 156.762 bits (395), Expect = 1.812e-42
Identity = 93/295 (31.53%), Postives = 154/295 (52.20%), Query Frame = 1 
Query:  292 FLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQC--------------FVSRASIREGGYTECSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKH--MKSKVKVIKMLSIIVICFGLCWLPL-HLFFVVTHVNQSQGYTKNLNI-AYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCCGL-CQRQSWSRKINFNTTSARN 1119
            F+ NLA+SDLL+ +FC+P T    ++  WPF  + C +   +Q ++V+AS  T + I +DRF  ++YP K ++T   +  +I  IWV ++   S+ C               V R + R   +  C ENW N  ++  YT  +    Y  PL ++ + Y  +GI L+K ++P       D +H   K K +VIKML I+ + F L WLPL  L  +  +V  ++   + +NI  Y   HW+A  NS VNP++Y F N++F+  F+ +     C +  Q +++S ++  N+    N
Sbjct:   84 FILNLAISDLLVGIFCMPTTLLDNIITGWPFGSMVCKMSGMVQGISVSASVFTLVAIAVDRFRCIVYPFKQKLTISTATFIIVIIWVLAV---SIMCPSGVMLQVTKEQNIRVFRGNNRSSPFYWCRENWPNQEMRKIYTTVLFANIYLAPLSLIVIMYARIGITLFKTAMPAGGKHGHDNRHSVSKKKQRVIKMLLIVALLFILSWLPLWTLMMLTDYVKLTEHQYRVINIYIYPFAHWLAFFNSSVNPIIYGFFNENFRRGFQAIFKFGLCPVGGQHRTYSHRVQGNSVQPVN 375
[Close]
BLAST of GCR009 vs. Ensembl Zebrafish
Match: NPFFR2 (neuropeptide FF receptor 2a [Source:NCBI gene;Acc:560955])

HSP 1 Score: 156.762 bits (395), Expect = 1.812e-42
Identity = 93/295 (31.53%), Postives = 154/295 (52.20%), Query Frame = 1 
Query:  292 FLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQC--------------FVSRASIREGGYTECSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKH--MKSKVKVIKMLSIIVICFGLCWLPL-HLFFVVTHVNQSQGYTKNLNI-AYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCCGL-CQRQSWSRKINFNTTSARN 1119
            F+ NLA+SDLL+ +FC+P T    ++  WPF  + C +   +Q ++V+AS  T + I +DRF  ++YP K ++T   +  +I  IWV ++   S+ C               V R + R   +  C ENW N  ++  YT  +    Y  PL ++ + Y  +GI L+K ++P       D +H   K K +VIKML I+ + F L WLPL  L  +  +V  ++   + +NI  Y   HW+A  NS VNP++Y F N++F+  F+ +     C +  Q +++S ++  N+    N
Sbjct:   84 FILNLAISDLLVGIFCMPTTLLDNIITGWPFGSMVCKMSGMVQGISVSASVFTLVAIAVDRFRCIVYPFKQKLTISTATFIIVIIWVLAV---SIMCPSGVMLQVTKEQNIRVFRGNNRSSPFYWCRENWPNQEMRKIYTTVLFANIYLAPLSLIVIMYARIGITLFKTAMPAGGKHGHDNRHSVSKKKQRVIKMLLIVALLFILSWLPLWTLMMLTDYVKLTEHQYRVINIYIYPFAHWLAFFNSSVNPIIYGFFNENFRRGFQAIFKFGLCPVGGQHRTYSHRVQGNSVQPVN 375
[Close]
BLAST of GCR009 vs. Ensembl Zebrafish
Match: NPFFR2 (neuropeptide FF receptor 2a [Source:NCBI gene;Acc:560955])

HSP 1 Score: 156.762 bits (395), Expect = 1.812e-42
Identity = 93/295 (31.53%), Postives = 154/295 (52.20%), Query Frame = 1 
Query:  292 FLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQC--------------FVSRASIREGGYTECSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKH--MKSKVKVIKMLSIIVICFGLCWLPL-HLFFVVTHVNQSQGYTKNLNI-AYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCCGL-CQRQSWSRKINFNTTSARN 1119
            F+ NLA+SDLL+ +FC+P T    ++  WPF  + C +   +Q ++V+AS  T + I +DRF  ++YP K ++T   +  +I  IWV ++   S+ C               V R + R   +  C ENW N  ++  YT  +    Y  PL ++ + Y  +GI L+K ++P       D +H   K K +VIKML I+ + F L WLPL  L  +  +V  ++   + +NI  Y   HW+A  NS VNP++Y F N++F+  F+ +     C +  Q +++S ++  N+    N
Sbjct:   84 FILNLAISDLLVGIFCMPTTLLDNIITGWPFGSMVCKMSGMVQGISVSASVFTLVAIAVDRFRCIVYPFKQKLTISTATFIIVIIWVLAV---SIMCPSGVMLQVTKEQNIRVFRGNNRSSPFYWCRENWPNQEMRKIYTTVLFANIYLAPLSLIVIMYARIGITLFKTAMPAGGKHGHDNRHSVSKKKQRVIKMLLIVALLFILSWLPLWTLMMLTDYVKLTEHQYRVINIYIYPFAHWLAFFNSSVNPIIYGFFNENFRRGFQAIFKFGLCPVGGQHRTYSHRVQGNSVQPVN 375
[Close]
BLAST of GCR009 vs. Ensembl Xenopus
Match: NPY2R (neuropeptide Y receptor Y2 [Source:NCBI gene;Acc:100498351])

HSP 1 Score: 150.214 bits (378), Expect = 5.425e-40
Identity = 100/286 (34.97%), Postives = 152/286 (53.15%), Query Frame = 1 
Query:  217 FGNVVVI-IVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASI----REGGYTECSENWNNNNLQIS--YTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKC 1053
             GN +VI +V    T R   N     F+ NLAVSDL+++  C+PFT  Y LL DW F  + C +V   Q LAV  S++T MVI LDR   ++Y ++S+I+ K   ++IG  WV S L +S        S+     +     CSE W   +L     Y+IS+LLI Y +PL +++  Y  +  KL     PG  +   D  H + + K  KML  +V+ F +CWLP H F + + ++          + Y + H IAM ++F NP++Y +MN+++++ F  L   KC
Sbjct:   89 LGNSLVIYVVIKFKTMRTVTNF----FIVNLAVSDLMVNTLCLPFTLVYTLLDDWKFGTVLCHLVTYSQGLAVQVSTVTLMVIALDRHRCIVYHLESKISTKICFMIIGITWVCSALLASPLAIFKEYSVIVISSDFQIQACSERWPVGDLNYGTIYSISMLLIQYIVPLAIISYAYIRIWTKLKNHVSPGGGN---DHYHQRRR-KTTKMLVAVVVVFAVCWLPFHAFQLASDIDGKVLDLNEYKLIYTIFHVIAMCSTFANPLLYGWMNNNYRTAF--LTAFKC 364
[Close]
BLAST of GCR009 vs. Ensembl Xenopus
Match: errfi1 (ERBB receptor feedback inhibitor 1 [Source:Xenbase;Acc:XB-GENE-1005334])

HSP 1 Score: 147.517 bits (371), Expect = 1.261e-39
Identity = 81/276 (29.35%), Postives = 146/276 (52.90%), Query Frame = 1 
Query:  217 FGNVVVIIVFWLGTCRPNRNNEVRQFLT-NLAVSDLLISLFCVPFTFTYILL-WDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSS-VQCFVSRASIREGGYTECSENW-NNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFR 1032
            FGN ++I V     CR  + + V  FL  NLA SD+L+   CVP T  Y+     W F +  C  V  +Q + V  S  T  VI +DR+   +YP++ R+T      ++  IW+ S L ++            +  ++ C E W      +++Y  S L++TY LP I++ ++Y  + +KL  R +PG    ++       + K  ++L ++V  FG+CWLPLH+F ++  ++      +  N+  ++CHW+AM ++  N ++Y++++DSF+ + +
Sbjct:   50 FGNYLLIYVI----CRTKKMHNVTNFLVGNLAFSDMLMCATCVPLTLAYVFEPRGWVFGHFLCYFVFLMQPVTVYVSVFTLTVIAVDRYHATVYPLRRRLTIPTCAYILAGIWLLSCLMAAPALAHTYHVEFPQLDFSICEEFWFGMEKKRLAYAYSTLILTYALPFIIIALSYLRISVKLKNRVVPGNITNVQAEWDRARRKKTFRLLVLVVGAFGMCWLPLHIFNIMKDIDIHLIDKQYFNLVQLLCHWLAMMSACTNSLLYAWLHDSFRGELK 321
[Close]
BLAST of GCR009 vs. Ensembl Xenopus
Match: ENSXETT00000044281.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:3:115219157:115249407:-1 gene:ENSXETG00000019365.1 transcript:ENSXETT00000044281.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 145.976 bits (367), Expect = 4.049e-39
Identity = 85/276 (30.80%), Postives = 143/276 (51.81%), Query Frame = 1 
Query:  217 FGNVVVIIVFWLGTCRPNRNNEVRQF-LTNLAVSDLLISLFCVPFTFTYIL-LWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWV-SSILFSSVQCFVSRASIREGGYTECSENWNNNNLQ-ISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFR 1032
            FGN +++ V     CR  + + V  F + NLA SD+L+   CVPFT  Y      W F    C  V  +Q + V  S  T   I +DR++  ++P+K RI+      V+  IW+ S  L             ++ G+T C E W     + ++Y  S L+ITY LPL  ++++Y  + +KL  R +PG   + +       K K+ +++ ++V  FG+CWLP+H+F ++  ++ +           + CHW AMS+S  NP +Y++++D F+S+ R
Sbjct:   50 FGNYLLLYV----ICRTRKMHNVTNFFIGNLAFSDMLMCATCVPFTLAYAFNPQGWVFGKFMCYFVFLMQPVTVYVSVFTLTAIAVDRYYATVHPLKKRISVTTCIYVLMGIWLLSCALVGPAIAHTYHVEFQQEGFTICEEFWMGKETERLAYAYSTLIITYILPLSAVSLSYICITVKLKNRVVPGHPTQSQAEFDRLRKRKIFRLIVLVVTAFGICWLPIHVFNIIRDIDINLINKHYFLHIQLFCHWFAMSSSCCNPFLYAWLHDRFRSELR 321
[Close]
BLAST of GCR009 vs. Ensembl Xenopus
Match: taf1b (TATA-box binding protein associated factor, RNA polymerase I, B [Source:Xenbase;Acc:XB-GENE-992275])

HSP 1 Score: 145.976 bits (367), Expect = 7.509e-39
Identity = 80/256 (31.25%), Postives = 141/256 (55.08%), Query Frame = 1 
Query:  292 FLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTE----------CSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDF 1029
            F+ NLA++DL++  FC+PFT  Y L+ +W F  + C +    Q ++V  S++T +VI LDR+W +++ + SRI++  S L+I   W+++ + +     +  A  RE  Y +          C+ENW N +  I Y++S+L++ Y LPL V+   Y  +  KL     P    E +     + +    KML ++V+ F +CWLP H+F +   +  +    +N  + Y + H +AM ++FVNP +Y +MN +++  F
Sbjct:   77 FIANLAIADLMVDSFCLPFTLVYTLMDEWKFGSVLCHLFPYAQAMSVNVSTLTLIVIALDRYWCIVFHLNSRISKNLSFLIISITWITAAILA-----IPLAVFREFRYEDLPPFNLKIAVCAENWTNRDSTI-YSLSMLILQYALPLAVICYAYLRIWFKLKNHISPTTRSESQ-----QRRKNTTKMLVMMVVVFAVCWLPFHIFQLAIDLEWTVAIHEN-KLLYTIFHVVAMCSTFVNPFLYGWMNKNYRHGF 320
[Close]
BLAST of GCR009 vs. Ensembl Xenopus
Match: clstn2 (calsyntenin 2 [Source:Xenbase;Acc:XB-GENE-958942])

HSP 1 Score: 140.969 bits (354), Expect = 1.880e-36
Identity = 90/297 (30.30%), Postives = 155/297 (52.19%), Query Frame = 1 
Query:  271 RNNEVR----QFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILF---SSVQCFVSRASIR--EGGYT------ECSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLP--GEADEIRDAKHM-KSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYV--VCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCCGLCQ--RQSWSRKIN 1095
            +N ++R     F+ NLA+SDLL+ +FC+P T    L+  WPF  + C +   +Q ++V+AS  T + I ++RF  ++YP + ++T +++ + I  IWV +++    S+V   V++         Y        C E W +  ++  YT  +    Y  PL ++ + Y  +  KL+K S P  G   E  + + + K KV+VI ML I+ + F L WLPL    ++T       Y  NL   YV    HW+A  NS VNP++Y + N++F+  F+     + C + +  R+++S + N
Sbjct:   65 KNRQMRTVTNMFILNLAISDLLVGIFCMPTTLVDNLITGWPFDNIMCKMSGLVQGMSVSASVFTLVAIAVERFRCIVYPFRQKLTLRKAIITIIIIWVLALIIMCPSAVTLTVTKDDFHFMVDDYNNYYPLYSCWEEWPDTEMRKIYTTVLFSHIYLAPLTLIVIMYARIAFKLFKSSAPIRGSVSEENEGRRVSKRKVRVIYMLIIVALFFSLSWLPLWTLMLLTDYGHLSDYQLNLIAVYVFPFAHWLAFFNSSVNPIIYGYFNENFRRGFQEAFKVQVCSMKREHRETYSERKN 361
[Close]
BLAST of GCR009 vs. Ensembl Mouse
Match: Tacr2 (tachykinin receptor 2 [Source:MGI Symbol;Acc:MGI:98477])

HSP 1 Score: 166.007 bits (419), Expect = 2.885e-46
Identity = 85/282 (30.14%), Postives = 151/282 (53.55%), Query Frame = 1 
Query:  220 GNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWNNNN---LQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCC 1056
            GN  VI   W+             F+ NLA++DL ++ F   F F Y     W F   FC       + A+  S  +   I  DR+  +++P + R++   +  VI  IW+ ++  +S QCF S  ++ +G  T+C   W N+N   + + Y + + ++ YFLPL+V+   Y+++G+ LWKR++P       + +H+++K K +K + ++V+ F +CWLP HL+F++    +   Y K +   Y+   W+AMS++  NP++Y  +N  F+S FR  +  +CC
Sbjct:   50 GNATVI---WIILAHERMRTVTNYFIINLALADLCMAAFNATFNFIYASHNIWYFGSTFCYFQNLFPVTAMFVSIYSMTAIAADRYMAIVHPFQPRLSAPSTKAVIAVIWLVALALASPQCFYSTITVDQGA-TKCVVAWPNDNGGKMLLLYHLVVFVLIYFLPLVVMFAAYSVIGLTLWKRAVPRHQAHGANLRHLQAKKKFVKAMVLVVVTFAICWLPYHLYFILGTFQEDIYYRKFIQQVYLALFWLAMSSTMYNPIIYCCLNHRFRSGFR--LAFRCC 325
[Close]
BLAST of GCR009 vs. Ensembl Mouse
Match: Tacr3 (tachykinin receptor 3 [Source:MGI Symbol;Acc:MGI:892968])

HSP 1 Score: 147.517 bits (371), Expect = 7.166e-39
Identity = 90/273 (32.97%), Postives = 149/273 (54.58%), Query Frame = 1 
Query:  217 FGNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWNNNNLQ-ISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFR 1032
            FGN++VI   W+             FL NLA SD  ++ F     F Y +  +W F   +C       + AV AS  +   I +DR+  +I P+K R++   + +VIG IW+ + L +  QC  S+  +  G  T C   W     Q  +Y I ++++ Y  PL+++ VTYTIVGI LW   +PG+  + +  + +K+K KV+KM+ I+V+ F +CWLP H++F++T + Q     K +   Y+   W+AMS++  NP++Y  +N  F++ F+
Sbjct:   88 FGNLIVI---WIILAHKRMRTVTNYFLVNLAFSDASVAAFNTLVNFIYGVHSEWYFGANYCRFQNFFPITAVFASIYSMTAIAVDRYMAIIDPLKPRLSATATKIVIGSIWILAFLLAFPQCLYSKIKVMPGR-TLCYVQWPEGPKQHFTYHIIVIILVYCFPLLIMGVTYTIVGITLWGGEIPGDTCD-KYHEQLKAKRKVVKMMIIVVVTFAICWLPYHVYFILTAIYQQLNRWKYIQQVYLASFWLAMSSTMYNPIIYCCLNKRFRAGFK 355
[Close]
BLAST of GCR009 vs. Ensembl Mouse
Match: Npy2r (neuropeptide Y receptor Y2 [Source:MGI Symbol;Acc:MGI:108418])

HSP 1 Score: 142.895 bits (359), Expect = 1.069e-37
Identity = 89/254 (35.04%), Postives = 141/254 (55.51%), Query Frame = 1 
Query:  292 FLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIW-VSSILFSSVQCFVSRASIR---EGGYTECSENWNNNNLQI---SYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEA-DEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDF 1029
            F+ NLAV+DLL++  C+PFT TY L+ +W    + C +V   Q LAV  S+IT  VI LDR   ++Y ++S+I+++ S L+IG  W +S++L S +  F   + I    +     C+E W      +    Y++S LLI Y LPL +++ +YT +  KL     PG A D     +H     K+ KML  +V+ F + WLPLH F +   ++      K   + + V H IAM ++F NP++Y +MN +++  F
Sbjct:   88 FIANLAVADLLVNTLCLPFTLTYTLMGEWKMGPVLCHLVPYAQGLAVQVSTITLTVIALDRHRCIVYHLESKISKRISFLIIGLAWGISALLASPLAIFREYSLIEIIPDFEIVACTEKWPGEEKSVYGTVYSLSTLLILYVLPLGIISFSYTRIWSKLRNHVSPGAASDHYHQRRH-----KMTKMLVCVVVVFAVSWLPLHAFQLAVDIDSHVLDLKEYKLIFTVFHIIAMCSTFANPLLYGWMNSNYRKAF 336
[Close]
BLAST of GCR009 vs. Ensembl Mouse
Match: Npy2r (neuropeptide Y receptor Y2 [Source:MGI Symbol;Acc:MGI:108418])

HSP 1 Score: 142.895 bits (359), Expect = 1.069e-37
Identity = 89/254 (35.04%), Postives = 141/254 (55.51%), Query Frame = 1 
Query:  292 FLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIW-VSSILFSSVQCFVSRASIR---EGGYTECSENWNNNNLQI---SYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEA-DEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDF 1029
            F+ NLAV+DLL++  C+PFT TY L+ +W    + C +V   Q LAV  S+IT  VI LDR   ++Y ++S+I+++ S L+IG  W +S++L S +  F   + I    +     C+E W      +    Y++S LLI Y LPL +++ +YT +  KL     PG A D     +H     K+ KML  +V+ F + WLPLH F +   ++      K   + + V H IAM ++F NP++Y +MN +++  F
Sbjct:   88 FIANLAVADLLVNTLCLPFTLTYTLMGEWKMGPVLCHLVPYAQGLAVQVSTITLTVIALDRHRCIVYHLESKISKRISFLIIGLAWGISALLASPLAIFREYSLIEIIPDFEIVACTEKWPGEEKSVYGTVYSLSTLLILYVLPLGIISFSYTRIWSKLRNHVSPGAASDHYHQRRH-----KMTKMLVCVVVVFAVSWLPLHAFQLAVDIDSHVLDLKEYKLIFTVFHIIAMCSTFANPLLYGWMNSNYRKAF 336
[Close]
BLAST of GCR009 vs. Ensembl Mouse
Match: Npy2r (neuropeptide Y receptor Y2 [Source:MGI Symbol;Acc:MGI:108418])

HSP 1 Score: 142.895 bits (359), Expect = 1.069e-37
Identity = 89/254 (35.04%), Postives = 141/254 (55.51%), Query Frame = 1 
Query:  292 FLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIW-VSSILFSSVQCFVSRASIR---EGGYTECSENWNNNNLQI---SYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEA-DEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDF 1029
            F+ NLAV+DLL++  C+PFT TY L+ +W    + C +V   Q LAV  S+IT  VI LDR   ++Y ++S+I+++ S L+IG  W +S++L S +  F   + I    +     C+E W      +    Y++S LLI Y LPL +++ +YT +  KL     PG A D     +H     K+ KML  +V+ F + WLPLH F +   ++      K   + + V H IAM ++F NP++Y +MN +++  F
Sbjct:   88 FIANLAVADLLVNTLCLPFTLTYTLMGEWKMGPVLCHLVPYAQGLAVQVSTITLTVIALDRHRCIVYHLESKISKRISFLIIGLAWGISALLASPLAIFREYSLIEIIPDFEIVACTEKWPGEEKSVYGTVYSLSTLLILYVLPLGIISFSYTRIWSKLRNHVSPGAASDHYHQRRH-----KMTKMLVCVVVVFAVSWLPLHAFQLAVDIDSHVLDLKEYKLIFTVFHIIAMCSTFANPLLYGWMNSNYRKAF 336
[Close]
BLAST of GCR009 vs. UniProt/SwissProt
Match: sp|P30975|TLR2_DROME (Tachykinin-like peptides receptor 99D OS=Drosophila melanogaster OX=7227 GN=TkR99D PE=2 SV=2)

HSP 1 Score: 170.244 bits (430), Expect = 9.621e-46
Identity = 114/375 (30.40%), Postives = 194/375 (51.73%), Query Frame = 1 
Query:  217 FGNVVVI------IVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRAS---IRE-GGYTECSENW-----NNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCCGL-CQRQSWSRKINFNTTSARNSVSNNFSPALTIQNR-KYNDKFPSRDHSSPERSKEIQFLFNKDNNECVNHNSIDLPRE 1290
            FG +V++      IV W+             F+ NL+++D ++S   V F + Y+L  DWPF   +C +   I +L++ AS  T M I +DR+  +I P++ R++++ +  +   IW++S L S     + R     +R     T C   W     N++ ++  Y I I+++TYFLP++ +TVTY+ VGI+LW     GE    R  ++++SK +V+KM+ ++V+ F +CWLP H +F++T    +      +   Y+  +W+AMSNS  NP++Y +MN  F+  F+ +V+  C  +    + +SR+ N    ++R S S   SP     NR K ND   S  ++ P   K  +   +         NS  LP E
Sbjct:  108 FGGMVIVATGGNLIVVWIVMTTKRMRTVTNYFIVNLSIADAMVSSLNVTFNYYYMLDSDWPFGEFYCKLSQFIAMLSICASVFTLMAISIDRYVAIIRPLQPRMSKRCNLAIAAVIWLASTLISCPMMIIYRTEEVPVRGLSNRTVCYPEWPDGPTNHSTMESLYNILIIILTYFLPIVSMTVTYSRVGIELWGSKTIGECTP-RQVENVRSKRRVVKMMIVVVLIFAICWLPFHSYFIITSCYPAITEAPFIQELYLAIYWLAMSNSMYNPIIYCWMNSRFRYGFK-MVFRWCLFVRVGTEPFSRRENL---TSRYSCSG--SPD---HNRIKRNDTQKSILYTCPSSPKSHRISHSGTGRSATLRNS--LPAE 470
[Close]
BLAST of GCR009 vs. UniProt/SwissProt
Match: sp|P30549|NK2R_MOUSE (Substance-K receptor OS=Mus musculus OX=10090 GN=Tacr2 PE=2 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 2.018e-45
Identity = 85/282 (30.14%), Postives = 151/282 (53.55%), Query Frame = 1 
Query:  220 GNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWNNNN---LQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCC 1056
            GN  VI   W+             F+ NLA++DL ++ F   F F Y     W F   FC       + A+  S  +   I  DR+  +++P + R++   +  VI  IW+ ++  +S QCF S  ++ +G  T+C   W N+N   + + Y + + ++ YFLPL+V+   Y+++G+ LWKR++P       + +H+++K K +K + ++V+ F +CWLP HL+F++    +   Y K +   Y+   W+AMS++  NP++Y  +N  F+S FR  +  +CC
Sbjct:   50 GNATVI---WIILAHERMRTVTNYFIINLALADLCMAAFNATFNFIYASHNIWYFGSTFCYFQNLFPVTAMFVSIYSMTAIAADRYMAIVHPFQPRLSAPSTKAVIAVIWLVALALASPQCFYSTITVDQGA-TKCVVAWPNDNGGKMLLLYHLVVFVLIYFLPLVVMFAAYSVIGLTLWKRAVPRHQAHGANLRHLQAKKKFVKAMVLVVVTFAICWLPYHLYFILGTFQEDIYYRKFIQQVYLALFWLAMSSTMYNPIIYCCLNHRFRSGFR--LAFRCC 325
[Close]
BLAST of GCR009 vs. UniProt/SwissProt
Match: sp|P16610|NK2R_RAT (Substance-K receptor OS=Rattus norvegicus OX=10116 GN=Tacr2 PE=2 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 3.019e-45
Identity = 90/306 (29.41%), Postives = 158/306 (51.63%), Query Frame = 1 
Query:  220 GNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWNNNN---LQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCC-----------GLCQRQSWSRKIN 1095
            GN  VI   W+             F+ NLA++DL ++ F   F F Y     W F   FC       + A+  S  +   I  DR+  +++P + R++   +  +I  IW+ ++  +S QCF S  ++ EG  T+C   W N+N   + + Y + + ++ YFLPL+V+   Y+++G+ LWKR++P       + +H+++K K +K + ++V+ F +CWLP HL+F++    +   Y K +   Y+   W+AMS++  NP++Y  +N  F+S FR  +  +CC            L    S SR++N
Sbjct:   50 GNATVI---WIILAHERMRTVTNYFIINLALADLCMAAFNATFNFIYASHNIWYFGRAFCYFQNLFPITAMFVSIYSMTAIAADRYMAIVHPFQPRLSAPSTKAIIAGIWLVALALASPQCFYSTITVDEGA-TKCVVAWPNDNGGKMLLLYHLVVFVLIYFLPLLVMFGAYSVIGLTLWKRAVPRHQAHGANLRHLQAKKKFVKAMVLVVLTFAICWLPYHLYFILGTFQEDIYYHKFIQQVYLALFWLAMSSTMYNPIIYCCLNHRFRSGFR--LAFRCCPWVTPTEEDRLELTHTPSLSRRVN 349
[Close]
BLAST of GCR009 vs. UniProt/SwissProt
Match: sp|P51144|NK2R_MESAU (Substance-K receptor OS=Mesocricetus auratus OX=10036 GN=TACR2 PE=2 SV=1)

HSP 1 Score: 165.622 bits (418), Expect = 3.020e-45
Identity = 90/306 (29.41%), Postives = 158/306 (51.63%), Query Frame = 1 
Query:  220 GNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWNNNN---LQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCC-----------GLCQRQSWSRKIN 1095
            GN  VI   W+             F+ NLA++DL ++ F   F F Y     W F   FC       + A+  S  +   I  DR+  +++P + R++   +   I  IW+ ++  +S QCF S  ++ +G  T+C   W N+N   + + Y + + ++ YFLPL+V+ V Y+++G+ LWKR++P       + +H+ +K K +K + ++V+ F +CWLP HL+F++    +   Y K +   Y+   W+AMS++  NP++Y  +N  F+S FR  +  +CC            L +  S SR++N
Sbjct:   50 GNATVI---WIILAHERMRTVTNYFIINLALADLCMAAFNATFNFVYASHNIWYFGRAFCYFQNLFPITAMFVSIYSMTAIAADRYMAIVHPFQPRLSAPITKATIAGIWLVALALASPQCFYSTITVDQGA-TKCVVAWPNDNGGKMLLLYHLVVFVLVYFLPLVVMFVAYSVIGLTLWKRAVPRHQAHGANLRHLHAKKKFVKAMVLVVLTFAICWLPYHLYFILGSFQKDIYYRKFIQQVYLALFWLAMSSTMYNPIIYCCLNHRFRSGFR--LAFRCCPWVTPTEEDRLELTRTPSLSRRVN 349
[Close]
BLAST of GCR009 vs. UniProt/SwissProt
Match: sp|P79218|NK2R_RABIT (Substance-K receptor OS=Oryctolagus cuniculus OX=9986 GN=TACR2 PE=2 SV=1)

HSP 1 Score: 165.622 bits (418), Expect = 3.280e-45
Identity = 85/282 (30.14%), Postives = 151/282 (53.55%), Query Frame = 1 
Query:  220 GNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENW---NNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCC 1056
            GN  VI   W+             F+ NLA++DL ++ F   F F Y     W F   FC       + A+  S  +   I  DR+  +++P + R++   +  VI  IW+ ++  +  QCF S  ++ +G  T+C   W   +   + + Y ++++ + YFLPL+V+ V Y+++G KLW+R++PG      + +H+++K K +K + ++V+ F +CWLP HL+F++ H        K +   Y+V  W+AMS++  NP++Y  +N  F+S FR  +  +CC
Sbjct:   50 GNATVI---WIILAHRRMRTVTNYFIVNLALADLCMATFNAAFNFVYASHNIWYFGRAFCYFQNLFPITAMFVSIYSMTAIAADRYMAIVHPFQPRLSGPGTKAVIAGIWLVALALAFPQCFYSTITMDQGA-TKCVVAWPEDSGGKMLLLYHLTVIALIYFLPLVVMFVAYSVIGFKLWRRTVPGHQTHGANLRHLRAKKKFVKTMVLVVVTFAVCWLPYHLYFLLGHFQDDIYCRKFIQQVYLVLFWLAMSSTMYNPIIYCCLNHRFRSGFR--LAFRCC 325
[Close]
BLAST of GCR009 vs. TrEMBL
Match: A0A193KUF7 (GCR009 OS=Schmidtea mediterranea OX=79327 GN=gcr009 PE=2 SV=1)

HSP 1 Score: 874.774 bits (2259), Expect = 0.000e+0
Identity = 442/442 (100.00%), Postives = 442/442 (100.00%), Query Frame = 1 
Query:   88 METTVANRFPNNSIKNGSDQIPWTFNEKVMXXXXXXXXXXXXXFGNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCCGLCQRQSWSRKINFNTTSARNSVSNNFSPALTIQNRKYNDKFPSRDHSSPERSKEIQFLFNKDNNECVNHNSIDLPREFQVKSKPLSTNMNKIKILILDAEVEKNRIFTNQNYQIITQL 1413
            METTVANRFPNNSIKNGSDQIPWTFNEKVMIIISYSTSISLSLFGNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCCGLCQRQSWSRKINFNTTSARNSVSNNFSPALTIQNRKYNDKFPSRDHSSPERSKEIQFLFNKDNNECVNHNSIDLPREFQVKSKPLSTNMNKIKILILDAEVEKNRIFTNQNYQIITQL
Sbjct:    1 METTVANRFPNNSIKNGSDQIPWTFNEKVMIIISYSTSISLSLFGNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCCGLCQRQSWSRKINFNTTSARNSVSNNFSPALTIQNRKYNDKFPSRDHSSPERSKEIQFLFNKDNNECVNHNSIDLPREFQVKSKPLSTNMNKIKILILDAEVEKNRIFTNQNYQIITQL 442
[Close]
BLAST of GCR009 vs. TrEMBL
Match: A0A210PF75 (Neuropeptide FF receptor 2 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT05422 PE=3 SV=1)

HSP 1 Score: 272.707 bits (696), Expect = 6.819e-83
Identity = 153/358 (42.74%), Postives = 221/358 (61.73%), Query Frame = 1 
Query:   70 FISN*IMETTVAN----RFPNNSIKNG------SDQIPWTFNEKVMXXXXXXXXXXXXXFGNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREG---GYTECSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQS---QGYTKNLNIAYVVC-HWIAMSNSFVNPVVYSFMNDSFKSDFRWLVY--HKCCGLCQRQSWSR 1086
            F+S  I   TVAN    R  +    +G      S+ IP + +    + I Y+ +   S+ GNV V++VF  G  R +R  ++R FL NLA +DL+++LFC+PFTF  ++L  W FP + CP+VL +Q+ +V AS  TNM IG+DRF  V +P++SR+T  R+  VI  IW+S+ +  SV  FV RA+   G   G  +C ENW  +  +  +TI I ++TY +PL++L +TY+IVGI LWKR+ PG  D IRD   ++SK+K++KML I+V  FG+CWLP+H+F +V     S   QG    L +A  +C HW+AMSNSF NPV+Y F N+SF++D   L Y    CCG C + +  R
Sbjct:   21 FVSGIITSKTVANMSNTRHNDTEFDSGAGNETFSEGIPMSDSTLTGLAILYTLTTVFSITGNVFVVLVFVRG--RRSRT-DLRPFLINLAAADLMMALFCMPFTFADVILGRWLFPEVLCPLVLFVQIFSVGASVFTNMAIGIDRFLAVTFPLRSRLTHARAKYVIIVIWISAFILGSVMFFVGRATETSGYGNGTQKCLENWPGDEARRIFTIIIFVVTYLVPLLILAITYSIVGIILWKRTAPGNKDHIRDMHRLRSKIKIVKMLVIVVAVFGICWLPIHVFAIVMDFYPSVFEQG-DHMLTMAVFLCVHWLAMSNSFANPVIYGFTNESFRADLATLFYMWMPCCG-CLKHAMPR 373
[Close]
BLAST of GCR009 vs. TrEMBL
Match: V4BK89 (G_PROTEIN_RECEP_F1_2 domain-containing protein (Fragment) OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_182876 PE=3 SV=1)

HSP 1 Score: 269.626 bits (688), Expect = 7.573e-83
Identity = 138/307 (44.95%), Postives = 200/307 (65.15%), Query Frame = 1 
Query:  118 NNSIKNGSDQIP-WTFNEKVMXXXXXXXXXXXXXFGNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENW--NNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIA---YVVCHWIAMSNSFVNPVVYSFMNDSFK 1020
            N+S+     +IP   +N KV++I  Y+     ++ GN + I++F  G        E+R FLTNLAV+DL++ +FC+PFT +Y ++ +W F    CPVV+ +Q ++VTAS  TNM IG+DRF+ V +P++ R+T  +S  VI  IW  S++ +SVQ  + R  +   G  +C E+W      L+ SY++ +L +TYFLPL +L+VTYTI+G  LWKR++PG +DE RDA  +KSK KV+KML  IV+ FGLCWLPLH F ++   N +     N N+    Y   HW+AMSNSFVNPV+Y FMN+SF+
Sbjct:   53 NDSLNRSVTEIPTLPYNSKVILITMYTFITVAAIIGNSIAIVIFTRG---KRSRTELRAFLTNLAVADLIMGIFCIPFTLSYQIIGEWVFTAPMCPVVIFLQYVSVTASVFTNMAIGIDRFYAVAFPLRKRLTSSKSKFVIITIWTFSVVINSVQLLIGRTEVDGAGRLQCGESWPYPQKELKQSYSLFVLFLTYFLPLCILSVTYTIIGRILWKRNVPGNSDETRDALQIKSKRKVVKMLVTIVLLFGLCWLPLHTFMLILDFNPT--LITNYNVQEKIYFGSHWLAMSNSFVNPVIYGFMNESFR 354
[Close]
BLAST of GCR009 vs. TrEMBL
Match: R7TCU3 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_131573 PE=3 SV=1)

HSP 1 Score: 268.855 bits (686), Expect = 8.420e-83
Identity = 138/272 (50.74%), Postives = 186/272 (68.38%), Query Frame = 1 
Query:  220 GNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWNN--NNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVV---THVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFK 1020
            GN++VI+VF +G        ++  FL NLAVSDL++++FC+PFTFT  +L  W F    CP+VL +Q ++VTAS  TNM IG+DRFWVV +P+KSR T+ RS +VI  IW  ++  SSVQ FV R ++R     +C E W       + +YT  IL +TY LPL +L+VTY IVG KLW+R+ PG ADE RD +  +SK KVIKML ++V+ FGLCWLPLH+F +V   + V Q +      +I Y V HW+AMSNSFVNP++YSF+NDSF+
Sbjct:   53 GNLIVIVVFSVGK---RSRTDLSGFLINLAVSDLIMAIFCLPFTFTMTMLETWVFSAPMCPIVLYMQTVSVTASVCTNMAIGVDRFWVVTFPLKSRFTKSRSKVVIAVIWAIALGLSSVQLFVGRVTVRSEDSVDCGEVWPQPTETWRRAYTFFILTLTYLLPLSILSVTYGIVGRKLWQRTAPGNADEARDTQQHRSKRKVIKMLVMVVLLFGLCWLPLHVFILVIDYSDVYQPENQHLIKSIYYAV-HWLAMSNSFVNPIIYSFLNDSFR 320
[Close]
BLAST of GCR009 vs. TrEMBL
Match: A0A0K0PUK8 (FMRFamide receptor 1 OS=Platynereis dumerilii OX=6359 PE=2 SV=1)

HSP 1 Score: 266.544 bits (680), Expect = 1.936e-80
Identity = 152/368 (41.30%), Postives = 226/368 (61.41%), Query Frame = 1 
Query:  220 GNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRA---SIREGGY-TECSENW--NNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVN-----QSQGYTKNLNIA-YVVCHWIAMSNSFVNPVVYSFMNDSFKSDF-----RWLVYHKCCGLCQRQSWSRK--INFNTTSARNSVSNNFSPALTIQNRKY---NDKFPSRDHSSPERSKEIQFLFNKDNNEC 1257
            GN++VIIVF  G       +E+R FL NLA +DL++++FC+PFTFT ++L +W F    CP+VL +Q ++VTAS  TNM IG+DRFWVV +P++SRIT+ RS +VI  +W+ +   SS+Q  + RA       G + T+C+E W    +  + +YT  IL++TY LPL +L++TY+IV ++LW+R+ PG AD+ RD++ ++SK KVIKML I+V+ FG+CWLPLH+F +V  ++      +   T  L +A Y   HW+AMSNSFVNP++Y F+NDSF++D      RW    K C      + +R   I F   S  +            ++RKY   N    SR +S+  R K++    N  N +C
Sbjct:   69 GNLIVIIVFIFGK---RSRSELRGFLINLACADLIMAVFCLPFTFTMVMLDNWIFSEPMCPIVLYMQTVSVTASVCTNMAIGIDRFWVVTFPLRSRITKSRSKIVIALVWLIAFGLSSIQLVIGRAVRTPYAPGLHRTDCAEVWFEPADTWRRAYTFFILILTYLLPLGILSLTYSIVAVRLWQRTAPGNADQARDSQQLRSKRKVIKMLVIVVVLFGVCWLPLHVFILVLDLHPYLLATAGPKTIQLFVALYYTAHWLAMSNSFVNPIIYGFLNDSFRNDLKRLFRRWCPCFKICPCLSSDALTRDSTIMFKECSVVDYP----------KDRKYSSSNGNGNSRRNSNDYR-KQLSVKLNGRNGKC 422
[Close]
BLAST of GCR009 vs. Ensembl Cavefish
Match: NPFFR2 (neuropeptide FF receptor 2 [Source:NCBI gene;Acc:103039748])

HSP 1 Score: 157.532 bits (397), Expect = 7.277e-43
Identity = 99/294 (33.67%), Postives = 158/294 (53.74%), Query Frame = 1 
Query:  292 FLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILF---SSVQCFVSRA-SIR----EGGYTE----CSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHM--KSKVKVIKMLSIIVICFGLCWLPL-HLFFVVTHVNQSQGYTKNLNI-AYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCC--GLCQRQSWSRKINFNTTSARN 1119
            F+ NLAVSDLL+ +FC+P T    ++  WPF  + C +   +Q ++V+AS  T + I +DRF  ++YP K ++T   + L+I  IWV +I     S V   V++  SIR    +G  T     C ENW N  ++  YT  +    Y  PL ++ + Y  +GI L+K ++P       D +H   K K +VIKML I+ + F L WLPL  L  +  +   ++   + +NI  Y   HW+A  NS VNP++Y F N++F+  F+ +   + C  G  +R+++S ++  N     N
Sbjct:   84 FILNLAVSDLLVGIFCMPTTLLDNIITGWPFGSMVCKMSGMVQGISVSASVFTLVAIAVDRFRCIVYPFKQKLTISTATLIIVIIWVLAISIMCPSGVMLQVTKEQSIRVFLGDGNRTSPFYWCRENWPNQEMRKIYTTVLFANIYLAPLSLIVIMYARIGITLFKTAVPTGGKPGNDNRHTVSKKKQRVIKMLLIVALLFILSWLPLWTLMMLTDYARLTEHQYRLINIYIYPFAHWLAFFNSSVNPIIYGFFNENFRRGFQAIFKFRLCTAGGHRRKTYSHRVQGNAVLPAN 377
[Close]
BLAST of GCR009 vs. Ensembl Cavefish
Match: npffr1l3 (neuropeptide FF receptor 1-like [Source:NCBI gene;Acc:111195007])

HSP 1 Score: 150.599 bits (379), Expect = 7.131e-40
Identity = 100/323 (30.96%), Postives = 156/323 (48.30%), Query Frame = 1 
Query:  292 FLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWV-------SSILFSSVQCFVSRASIREGGYT------ECSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMK-----SKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYV--VCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCCGLCQRQSWSR-KINFNTTSARNSV-------SNNFSPALTIQNRKYND 1176
            F+ NL++SDLL+ +FC+P T    L+  WPF  + C +   +Q  +V AS  T + I +DRF  ++YP K ++T   +   IG IW+        S L  +V+      ++  G Y        C E W +  ++  YT  +    Y +PL+++ + Y  +G KL+  ++    ++     H K      K+KVIKML ++ + F L WLPL    ++T   + +G    L   YV    HW+A +NS VNP++Y + N++FK  F  +     C  C R   SR  I      A  S        SNN S  L ++NR Y D
Sbjct:   96 FILNLSISDLLVGIFCIPTTLVDNLITGWPFSDVMCKLSGLVQGTSVCASVFTLVAIAVDRFRCIVYPFKPKLTLLVAKATIGLIWLLALVIMFPSALMLTVEQERGHFTVYNGDYNLSYPLYSCYETWPDPQMRKVYTTVLFAHIYLVPLVLIILMYGRIGAKLYTTTVLVNVEQADGTPHGKCPVSQRKIKVIKMLIVVALLFMLSWLPLWTLMLLTDYARPEGEQLELLTGYVFPFSHWLAFANSSVNPIIYGYFNENFKRGFHAVCQQTSC--CSRGRHSRLGIKRPERGAEVSAHLDKTLNSNNLS--LGVRNRVYTD 414
[Close]
BLAST of GCR009 vs. Ensembl Cavefish
Match: npffr2b (neuropeptide FF receptor 2-like [Source:NCBI gene;Acc:103031865])

HSP 1 Score: 149.443 bits (376), Expect = 1.284e-39
Identity = 96/295 (32.54%), Postives = 157/295 (53.22%), Query Frame = 1 
Query:  292 FLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILF---SSVQCFVSRAS-----IREGGYTE----CSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLP---GEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPL-HLFFVVTHVNQSQGYTKNLNI-AYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCCGL--CQRQSWSRKINFNTTSARN 1119
            F+ NLA+SDLL+ +FC+P T    ++  WPF  L C +   +Q ++V+AS  T +VI +DRF  ++YP K ++T   S ++I  IWV +I     S V   V++ S     + E   T     C ENW +  ++  YT  +    Y  PL ++ + Y  +GI L+K ++P       E R     + K +VIKML ++ + F L WLPL  L  +  + + S+   + +NI  Y + HW+A  NS VNP++Y F N++F+  F+ +   + C     +R+S+S ++  N     N
Sbjct:   86 FILNLAISDLLVGIFCMPTTLVDNIITGWPFGSLVCKLSGMVQGISVSASVFTLVVIAVDRFRCIVYPFKQKLTISTSTVIIVIIWVLAISIMCPSGVMLQVTKESRVSILLGESNATRPFYWCRENWPSQEMRKIYTTVLFANIYLAPLTLIVIMYARIGITLFKTAVPVPTRGGYEGRGHTVSRKKKRVIKMLLVVALLFILSWLPLWTLMMLSDYASLSEYQHRVINIYVYPLAHWLAFCNSSVNPIIYGFFNENFRRGFQIVFKLQFCSAQRGKRRSYSHRVQGNAVLPTN 380
[Close]
BLAST of GCR009 vs. Ensembl Cavefish
Match: npy2rl (neuropeptide Y receptor type 2-like [Source:NCBI gene;Acc:103047056])

HSP 1 Score: 144.436 bits (363), Expect = 1.974e-38
Identity = 94/282 (33.33%), Postives = 147/282 (52.13%), Query Frame = 1 
Query:  220 GNVVVIIVFWLGTCRPNRNNEVRQF-LTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWV-SSILFSSVQCFVSRASIREGGYTE---------CSENWNNNNLQIS-YTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDF 1029
            GN VVI V     CR      V  F + NLAV+DLL++  C+PFT  Y LL +W F  + C  +   Q LAV  S++T  VI LDR   ++Y +++R+++    L+I   WV S++L S +  F      RE G  E         C E W  ++   + Y+IS+LL+ Y LPL V++  Y    I++W + L             + + K  KML  +V+ F + WLP H F +   ++ S    ++  + Y V H +AM ++F NP++Y +MN +++S F
Sbjct:   61 GNSVVIYV----VCRFKTLRTVTNFFIANLAVADLLVNTLCLPFTLAYTLLGEWKFGQVLCFTLPYAQGLAVHVSTVTLNVIALDRHRCIVYHLETRMSKDTCFLIIAITWVISAVLASPLAIF------REYGMVELSPGESIEVCGEKWPGSSTDGTLYSISMLLLQYVLPLAVISFAY----IRIWSK-LRNHVGPAERNDRHRRRRKTTKMLVTMVVVFAVSWLPFHAFQLAIDIDSSVLVMRDFRLLYTVFHIVAMCSTFANPILYGWMNRNYRSAF 327
[Close]
BLAST of GCR009 vs. Ensembl Cavefish
Match: npy2rl (neuropeptide Y receptor type 2-like [Source:NCBI gene;Acc:103047056])

HSP 1 Score: 144.436 bits (363), Expect = 1.974e-38
Identity = 94/282 (33.33%), Postives = 147/282 (52.13%), Query Frame = 1 
Query:  220 GNVVVIIVFWLGTCRPNRNNEVRQF-LTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWV-SSILFSSVQCFVSRASIREGGYTE---------CSENWNNNNLQIS-YTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDF 1029
            GN VVI V     CR      V  F + NLAV+DLL++  C+PFT  Y LL +W F  + C  +   Q LAV  S++T  VI LDR   ++Y +++R+++    L+I   WV S++L S +  F      RE G  E         C E W  ++   + Y+IS+LL+ Y LPL V++  Y    I++W + L             + + K  KML  +V+ F + WLP H F +   ++ S    ++  + Y V H +AM ++F NP++Y +MN +++S F
Sbjct:   61 GNSVVIYV----VCRFKTLRTVTNFFIANLAVADLLVNTLCLPFTLAYTLLGEWKFGQVLCFTLPYAQGLAVHVSTVTLNVIALDRHRCIVYHLETRMSKDTCFLIIAITWVISAVLASPLAIF------REYGMVELSPGESIEVCGEKWPGSSTDGTLYSISMLLLQYVLPLAVISFAY----IRIWSK-LRNHVGPAERNDRHRRRRKTTKMLVTMVVVFAVSWLPFHAFQLAIDIDSSVLVMRDFRLLYTVFHIVAMCSTFANPILYGWMNRNYRSAF 327
[Close]
BLAST of GCR009 vs. Ensembl Sea Lamprey
Match: prlh2r (prolactin releasing hormone 2 receptor [Source:ZFIN;Acc:ZDB-GENE-120411-41])

HSP 1 Score: 144.436 bits (363), Expect = 1.643e-39
Identity = 86/275 (31.27%), Postives = 145/275 (52.73%), Query Frame = 1 
Query:  220 GNVVVIIVFWLGTCRPNRNNEVRQFLT-NLAVSDLLISLFCVPFTFTYIL-LWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSR-ASIREGGYTECSENW-NNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFR 1032
            GN +++ V     CR  + + V   L  NLA SDLL+   CVP T  Y+     W F    C  V  +Q ++V  S  T  VI +DR+ V +YP++ RIT    G ++  IWV++   ++     +      E   T C E W      +++Y  S L++TY LPL+V++++Y  + +KL  R +PG   + +       + K  ++L ++V  F +CWLPLH+F  +  V+         N+  ++CHW AMS++  NP +Y++++D F+S+ +
Sbjct:   38 GNFLLVYVI----CRVKKMHNVTNLLIGNLAFSDLLMCAMCVPLTLAYVFDARGWVFGSFMCYFVFLMQPVSVYVSVFTLTVIAVDRYIVTVYPLRRRITIPTCGYLLAAIWVTACALAAPALAHTYYCEFPEQRMTICEEFWIGTERERLAYAYSTLVLTYGLPLLVISLSYFKISVKLKNRVVPGTVTQNQAEMDRARRRKTFRLLVLVVTAFSVCWLPLHIFNFLRDVDIRLIDKYYFNLVQLLCHWFAMSSACYNPFIYAWLHDRFRSELK 308
[Close]
BLAST of GCR009 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008040.1 (pep scaffold:Pmarinus_7.0:GL477351:476361:492361:-1 gene:ENSPMAG00000007251.1 transcript:ENSPMAT00000008040.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 138.658 bits (348), Expect = 6.673e-37
Identity = 86/275 (31.27%), Postives = 146/275 (53.09%), Query Frame = 1 
Query:  217 FGNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWNN---NNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFR 1032
            FGN+VVI   W+             FL NLAV+D  ++       F Y +  DW F   +C     I + AV AS  +   I +DR+  + +P+K R+T + + LV+  IW  ++  S      SR   R G +  C  +W      + Q  Y + ++ + Y  PL+V+ VTYTIVG+ LW  ++PG++ E    + ++SK KV+KM+ ++V+ F +CW+P H++F++        + K +   Y+   W+AMS++  NP++Y  +N  F++ F+
Sbjct:   73 FGNMVVI---WIVVAHRRMRTVTNYFLVNLAVADSAMAALNTSINFVYAVHNDWYFGGAYCRFQNFIPVAAVFASIYSMTAIAVDRYMAIRHPLKPRMTAQSTRLVMAGIWALAMCLSLPLSIYSRVEQRFGRFV-CLVDWPGKMGGDSQFIYQLVVMTLVYVFPLLVMAVTYTIVGVTLWGGTIPGDSSE-HFREQLRSKRKVVKMMIVVVVTFAVCWMPYHVYFLLGRTLNRIYHRKYIQQVYLAIFWLAMSSTMYNPIIYCCLNSRFRTGFK 342
[Close]
BLAST of GCR009 vs. Ensembl Sea Lamprey
Match: qrfpr4 (pyroglutamylated RFamide peptide receptor 4 [Source:ZFIN;Acc:ZDB-GENE-091204-354])

HSP 1 Score: 130.568 bits (327), Expect = 3.290e-34
Identity = 90/299 (30.10%), Postives = 154/299 (51.51%), Query Frame = 1 
Query:  220 GNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPM--KSRITRKRSGLVIGCIW-----VSSILFSSVQCFVSRASIREGGYTECSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEA-------DEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLF-FVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCCGLCQR 1071
            GN +VI V      R   +     F+ +LAVSDL+I++FC+P T       +W      C +V  IQ+ AVT S +T   I ++RF  +++P+  +SR T  R+  ++  +W     V++ +F +    V+   + +  +T CSE W++   + +YT ++LL+T+ LPL+ +T  YT +  +LW ++   +A        EI   K  +SK + +KM+ I+VI F +CW P H+   +V   N    +     I   +      SNS  NP+VY+ +N ++K   + L     C +C+R
Sbjct:   62 GNALVIFVV---ARRKALHTVANVFVCSLAVSDLMITVFCIPITLMQNFFSNWIAGVALCKLVPFIQVTAVTTSMLTMACIAVERFQGILHPLRSRSRYTTARAASMLCGVWLVGLCVAAPMFYAHTVQVTYDVLYDVSHTGCSEQWSHAEHRQAYTTTLLLVTFVLPLLTMTALYTRLAHELWVKTRVHDAVFNALQRSEIN--KITRSKKRAVKMMVIVVILFAVCWAPFHVVNSLVPKGNLEMSFDME-AITLAMVQLFGFSNSLWNPIVYAALNQNYK---KQLAIALSCLMCRR 351
[Close]
BLAST of GCR009 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010661.1 (pep scaffold:Pmarinus_7.0:GL479584:296:6514:-1 gene:ENSPMAG00000009652.1 transcript:ENSPMAT00000010661.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 128.257 bits (321), Expect = 4.630e-34
Identity = 82/208 (39.42%), Postives = 123/208 (59.13%), Query Frame = 1 
Query:  220 GNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSI---LFSSVQCFVSRASIREGGYTECSE-NWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKML 831
            GN VV+ V  LG        ++  FL NLA++DL ++LFC+PFTFT +LL  W F  L CP+V   Q L+V+ S  T   IG+DR++ V +P+ SR+T+ R  ++I  IW  SI   L ++     +R  + + G  +C E  W +   Q SYT+++LL TY LPL+VLT+ Y+ V ++LW R  PG  D  R+    +SK K+  ++
Sbjct:   16 GNAVVVWVLALGR---RARTDISAFLINLALADLTMALFCLPFTFTEVLLQSWLFGALLCPLVRFAQTLSVSVSIYTLTTIGIDRYYAVCHPLHSRMTKSRGKVLIALIWAVSISISLSTTTLAVATRGPMLQLGKGKCLEVGWPSRGYQQSYTVALLLGTYLLPLLVLTLAYSSVAVRLWGRRTPGNVDHNRELHQERSKRKLCHLV 220
[Close]
BLAST of GCR009 vs. Ensembl Sea Lamprey
Match: npffr1l3 (neuropeptide FF receptor 1 like 3 [Source:ZFIN;Acc:ZDB-GENE-070424-44])

HSP 1 Score: 126.716 bits (317), Expect = 8.122e-33
Identity = 82/272 (30.15%), Postives = 130/272 (47.79%), Query Frame = 1 
Query:  292 FLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYT-------------ECSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKL----------WKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPL-HLFFVVTHVNQSQGYTKNLNI-AYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFR 1032
            F+ NLAVSDLL+ +FC+P T    L+  WPF  + C +   +Q  +V+AS  T + I +DRF  ++YP K ++    +   I  IW  ++        +  A +  G Y              +C E W +  ++ +YT  +    Y  PL ++ + Y  +G +L             + P            + +VKVIKML I+ + F L WLPL  +  +V + N S      LN+  Y V HW+A  NS VNP+VY + N++F+  F+
Sbjct:   87 FILNLAVSDLLVGIFCMPITLVDNLITGWPFGVVVCKMSGLVQGTSVSASVFTLVAIAVDRFRCIVYPFKRKLRISHALFTILVIWALALSIMCPSALMLTAEVSRGHYMVYSDGSNDTHPLHQCWEAWPDPRMRKAYTTVLFANVYLAPLALILLMYARIGFRLCSLSASAAAAASAAAPSRGGGGGATGVTRRRVKVIKMLIIVAMLFMLSWLPLWTVMMLVDYANLSPWDLAFLNMYVYPVAHWLAFFNSSVNPIVYGYFNENFRRGFQ 358
[Close]
BLAST of GCR009 vs. Ensembl Nematostella
Match: EDO39719 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S9A0])

HSP 1 Score: 128.257 bits (321), Expect = 7.499e-34
Identity = 84/282 (29.79%), Postives = 147/282 (52.13%), Query Frame = 1 
Query:  220 GNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYIL-LWDWPFPY-----LFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIRE-GGYTECSENW----NNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFR 1032
            GN +V+   W         N +   + N+A++DLL  +  +P TF  I   ++WPF Y      FC VV   Q L V  S +T   I  DRF+ +  PMK   T+K    ++  IW+ +   ++   +  R  I+E  G   C E+W    +       YTI +  + Y +P +V++V Y+++  +LW R +PGE D  +  K ++S+ KV+KML  +V+CF LCWLP+    V T +    G   ++++ +++  ++  S+S V+P +Y+  + +++  F+
Sbjct:    9 GNFLVVHTVWTNQRMQTATNYL---IVNMAIADLLYMVLVIPPTFIVIYGSYEWPFRYSGRAIYFCQVVHFGQYLLVPVSVLTLATIATDRFFAITMPMKRLFTKKVYRSLMVVIWLVAAGLAAPILYAYR--IKEFSGMLYCDEDWSPAFDTTRASRVYTIFLFAVAYCVPFVVMSVMYSVICRQLWMRKIPGEQDIEKSKKALESRKKVVKMLITVVVCFVLCWLPVQ---VATFLWDYSGVEISVDLNFIL-MFLMRSHSAVSPCIYAIFSQNYRQGFK 281
[Close]
BLAST of GCR009 vs. Ensembl Nematostella
Match: EDO47535 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RM40])

HSP 1 Score: 128.257 bits (321), Expect = 1.159e-33
Identity = 88/291 (30.24%), Postives = 149/291 (51.20%), Query Frame = 1 
Query:  217 FGNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYI---LLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASI-REGGYTECSENWNNNN--------LQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPG--EADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLN------IAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDF 1029
            FGN +VI +      R          + N+A++DLL+S+  +P  F Y     + DW    + C  V     L++TAS I  + I LDR++ +I+P K     + + L+   IW+ S LF+ +   +   ++ R+ G  EC  NWN  +           +Y ++ L++ Y +PL+V+   Y ++G KLW R +PG   A+  R A+   SK KV++ML I+V+ F LCWLP H+  ++ + +    +    N      I + +CH    +NS +NP++Y  +N  F+ +F
Sbjct:    7 FGNAIVIHIV---ATRAYMKTTFNFLIVNMAIADLLVSVMVMPLQFLYYGQQWISDW-IGSVTCKWVNFSGTLSITASIIALVAISLDRYFAIIHPFKHLPIIRNTKLITSFIWIVSALFNVLYLVLFEVNLGRKSGVWECHMNWNTISRDGKEQFEFARTYFMATLILLYIVPLVVIGTLYVLIGRKLWSRKIPGVTTANVKRYAE--TSKRKVLRMLIIVVVVFALCWLPAHIMHIIIYYHSDIYFQIVQNTPIVESIVFFLCH----ANSAINPILYVVLNKKFQREF 287
[Close]
BLAST of GCR009 vs. Ensembl Nematostella
Match: EDO44418 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVN5])

HSP 1 Score: 126.716 bits (317), Expect = 2.817e-33
Identity = 81/276 (29.35%), Postives = 139/276 (50.36%), Query Frame = 1 
Query:  220 GNVVVIIVFWLGTCRPNRNNEVRQ-FLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENW----NNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFR 1032
            GN VVI       CR      V   F+ NLA SDLLI+   +P   T +   +W F  + C +V+ +Q ++V +S +T + I  DRF  +I+P + R++ K +  +I  IW  S+   +   + ++         +C+E+W    +       YT+ + ++ Y +P++ + V Y I+  KLW+R   GE      +K+  S+ KVIKML  +++ F   WLPLH+F  ++        T N  + Y V  ++   NS  NP +Y+  N +++  F+
Sbjct:   13 GNTVVI----FTVCRNRHMKSVTNLFIANLAASDLLITFLGMPHQITELYTTEWMFGTVLCKLVVYLQSVSVGSSVLTLLAITKDRFVAIIFPFRPRMSFKTAKTIIMLIWSLSLGIMAPLIYATKVH-----NNQCTEDWTPAFDAKTAPRDYTVVLFVMLYAVPMLTMAVLYAIIVRKLWRRRRIGEVIPSASSKNEVSRKKVIKMLITVIMLFTFSWLPLHVFSFISFFAPFCPDTSNTAL-YFVSLFLGHGNSAANPYLYALFNQNYRDGFK 278
[Close]
BLAST of GCR009 vs. Ensembl Nematostella
Match: EDO39715 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S994])

HSP 1 Score: 125.561 bits (314), Expect = 5.603e-33
Identity = 82/259 (31.66%), Postives = 137/259 (52.90%), Query Frame = 1 
Query:  292 FLTNLAVSDLLISLFCVPFTFT-YILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWN--------NNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVV---CHWIAMSNSFVNPVVYSFMNDSFKSDFR 1032
             + NLA++DL + L C+PFT   Y L + WPF    C ++  +Q L + AS  T M I +DR   ++YP   RI+  +  L+I  +W+  +L +     V        G T C+E W         N+ L  +YTI+  ++TY +PL ++ + Y  + +KL + ++   AD        +++ K+IKML  +V+C+ LC+LP H    +  +N SQ   +N  I Y++   C  I   NS  NP++Y+F+ D F++ F+
Sbjct:    6 LIANLAIADLGVGLLCIPFTVVFYELNFVWPFGAFMCKMI-CLQPLFLMASVATLMAISIDRHQSIVYPFNPRISLTQGKLIIALVWLIGMLPAFPMLGVLELVETPRGPT-CTETWPDMDFGATINSKLSFAYTIASFVLTYAVPLPLIIILYIRIALKL-REAIKDAADRPGFQAAQQTR-KIIKMLVAVVVCYALCYLPFH---GMVFLNYSQ-VVENTPIVYLIQSYCQVIMYFNSASNPILYAFLGDQFRNGFK 256
[Close]
BLAST of GCR009 vs. Ensembl Nematostella
Match: EDO49570 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGN9])

HSP 1 Score: 124.02 bits (310), Expect = 3.020e-32
Identity = 78/254 (30.71%), Postives = 137/254 (53.94%), Query Frame = 1 
Query:  292 FLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWNN--NNLQI--SYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHM-KSKVKVIKMLSIIVICFGLCWLPLHLF-FVVTHVNQS-QGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFR 1032
            F+ NLA SD+LI+   +P   T   +  W F  + C +V+ +Q ++V +S +T + + +DRF  +++P++ RI   ++  ++   W+ S+   +      +A     G   C E W    N L+   S+T+ + ++ Y  PL+ + V Y  +   LWKR+ PG A   R   H+ +S+ +V+KML  + + F +CWLPL +F F++    Q+   ++K     Y V  ++  SNS +NP +YSF N +F+  FR
Sbjct:   33 FVANLAASDILITFTGMPNMITEQYIRHWVFGPIVCKLVVYLQSVSVASSVLTLLAVTIDRFISIVFPLRGRIALSKAKYIVAVTWIISLSIMAPLIDAQKAKQFPDGMYYCIEEWQPPFNPLEAPKSFTVLLFVVMYVAPLLTMAVLYCAICRTLWKRTSPG-ARITRTETHLQRSRKRVLKMLLAVTLLFAVCWLPLWIFQFIMFFSPQTFPCFSKPF---YFVSLFLGHSNSAINPFLYSFFNANFREGFR 282
[Close]
BLAST of GCR009 vs. Ensembl Medaka
Match: ENSORLT00000042596.1 (neuropeptide FF receptor 2 [Source:NCBI gene;Acc:101169169])

HSP 1 Score: 149.443 bits (376), Expect = 1.759e-39
Identity = 89/302 (29.47%), Postives = 156/302 (51.66%), Query Frame = 1 
Query:  292 FLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILF---SSVQCFVSRASI---------REGGYTECSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKH--MKSKVKVIKMLSIIVICFGLCWLPL-HLFFVVTHVNQSQGYTKNLNI-AYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCCGL--CQRQSWSRKINFNTTSARNSVSNNFSP 1143
            F+ NLAVSDLL+ +FC+P T    ++  WPF  L C +   +Q ++V+AS  T + I +DRF  ++YP K ++T   + ++I  IWV ++     S V   V++            +   +  C E+W N  ++  YT  +    Y  PL ++ + Y  +GI L+K ++P      ++ +H   K K +VIKML ++ + F + WLPL  L  +  +   ++   + +NI  Y   HW+A  NS VNP++Y F N++F+  F+ +     C     +R+++S ++  N+    N +     P
Sbjct:  130 FILNLAVSDLLVGIFCMPTTLLDNIITGWPFGSLVCKMSGMVQGISVSASVFTLVAIAVDRFRCIVYPFKQKLTISTATMIIVIIWVLAVSIMCPSGVMLQVTKEHTVRVLLGYDNKSSPFYWCKEDWPNQEMRKIYTTVLFANIYLAPLSLIVIMYARIGITLFKTTVPTGGKPGQENRHNVSKKKQRVIKMLLVVALLFIMSWLPLWTLMMLSDYARLTEQQYRIINIYVYPFAHWLAFFNSSVNPIIYGFFNENFRRGFQAVFKFSLCAADGHRRRTYSHRLQGNSVLPANPLQTTVEP 431
[Close]
BLAST of GCR009 vs. Ensembl Medaka
Match: npffr1l2 (neuropeptide FF receptor 1 like 2 [Source:ZFIN;Acc:ZDB-GENE-060526-110])

HSP 1 Score: 149.443 bits (376), Expect = 1.779e-39
Identity = 104/368 (28.26%), Postives = 187/368 (50.82%), Query Frame = 1 
Query:  220 GNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILF---SSVQCFVSRAS----IREGGYTE------CSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSL----PGEADEIRDAKHMKS--KVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYV--VCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHK--CCGLC--------QRQSWSRKINFNTTSARNSVSNNFSPALTIQNRKYNDKFPSRDHSSPERSKEIQF 1230
            GN++V ++     C     N    F+ NLA+SDLL+ +FC+P T    L+  WPF    C +   +Q ++V+AS  T + I ++RF  ++YP++ ++T   + + I  IWV +++    ++V   V +      +    + +      C EN+ N  ++  YT  +    Y LPL V+T+ Y  +G+KL    +    P  A+  R  + M S  K+KV+KML ++ + F + WLPL    ++T          +L  +Y+    HW+A SNS VNP+VY + N +FK  F+ +   +  CC L          R+  S++  F  + +R+  +N+ +  L ++NR +ND   ++ +  PE ++ ++ 
Sbjct:   67 GNILVCLIVLENRCMRTVTN---LFILNLAISDLLVGIFCIPTTLVDNLITGWPFSNTVCKMSGFVQGVSVSASVFTLVAIAVERFRCIVYPLQPKLTLLVAKVAIVSIWVLAVVILCPAAVALTVEKVPFHYMVHNNDFNQTLPLYTCYENFANPQMRKIYTAVLFAHIYLLPLAVITLMYISIGVKLCSSVVANREPQLANIRRHGQQMVSQKKIKVVKMLVVVTLLFMVSWLPLWTLMMMTDYGGLDRDQLDLLTSYIFPFAHWLAFSNSSVNPIVYGYYNKNFKRGFQSVCKSRPFCCNLQLKRRMARWGRKGQSQQAPFGGSDSRDGTNNHNNFVLGLRNRVHND---NKLNDMPEGNRGMKM 428
[Close]
BLAST of GCR009 vs. Ensembl Medaka
Match: npffr1l2 (neuropeptide FF receptor 1 like 2 [Source:ZFIN;Acc:ZDB-GENE-060526-110])

HSP 1 Score: 149.443 bits (376), Expect = 1.961e-39
Identity = 104/368 (28.26%), Postives = 187/368 (50.82%), Query Frame = 1 
Query:  220 GNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILF---SSVQCFVSRAS----IREGGYTE------CSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSL----PGEADEIRDAKHMKS--KVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYV--VCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHK--CCGLC--------QRQSWSRKINFNTTSARNSVSNNFSPALTIQNRKYNDKFPSRDHSSPERSKEIQF 1230
            GN++V ++     C     N    F+ NLA+SDLL+ +FC+P T    L+  WPF    C +   +Q ++V+AS  T + I ++RF  ++YP++ ++T   + + I  IWV +++    ++V   V +      +    + +      C EN+ N  ++  YT  +    Y LPL V+T+ Y  +G+KL    +    P  A+  R  + M S  K+KV+KML ++ + F + WLPL    ++T          +L  +Y+    HW+A SNS VNP+VY + N +FK  F+ +   +  CC L          R+  S++  F  + +R+  +N+ +  L ++NR +ND   ++ +  PE ++ ++ 
Sbjct:   63 GNILVCLIVLENRCMRTVTN---LFILNLAISDLLVGIFCIPTTLVDNLITGWPFSNTVCKMSGFVQGVSVSASVFTLVAIAVERFRCIVYPLQPKLTLLVAKVAIVSIWVLAVVILCPAAVALTVEKVPFHYMVHNNDFNQTLPLYTCYENFANPQMRKIYTAVLFAHIYLLPLAVITLMYISIGVKLCSSVVANREPQLANIRRHGQQMVSQKKIKVVKMLVVVTLLFMVSWLPLWTLMMMTDYGGLDRDQLDLLTSYIFPFAHWLAFSNSSVNPIVYGYYNKNFKRGFQSVCKSRPFCCNLQLKRRMARWGRKGQSQQAPFGGSDSRDGTNNHNNFVLGLRNRVHND---NKLNDMPEGNRGMKM 424
[Close]
BLAST of GCR009 vs. Ensembl Medaka
Match: prlhr2b (prolactin-releasing peptide receptor-like [Source:NCBI gene;Acc:101164576])

HSP 1 Score: 144.821 bits (364), Expect = 1.316e-38
Identity = 87/269 (32.34%), Postives = 141/269 (52.42%), Query Frame = 1 
Query:  259 CRPNRNNEVRQF-LTNLAVSDLLISLFCVPFTFTYIL-LWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSI-LFSSVQCFVSRASIREGGYTECSENW-NNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKC 1053
            CR  + + V  F + NLA SD+L+ + CVPFT  Y      W F    C +V  IQ + V  S  T   I +DR++  ++P+K R T      V+  IW+ S  L +           +E G+T C E W      + +Y  S LLITY LPL  + V+Y  + +KL K   PG   + +   H   K K+ ++++++V  F +CWLP+H+F V+  ++      +   +  ++CH  AMS+S  NP +Y++++D F+S+ R ++  KC
Sbjct:   80 CRTRKMHNVTNFFIGNLAFSDMLMCVTCVPFTLAYAFNPHGWVFGRSMCYLVFLIQPVTVYVSVFTLTAIAVDRYYATVHPLKKRTTVATCIYVLSGIWLLSCGLVAPAVSHTYHVEFKEEGFTICEEFWLGQEKERRAYAYSTLLITYVLPLSAVFVSYLCITVKLKKCVAPGNKTQDQVGIHQARKRKIFRLVALLVSAFAVCWLPIHVFNVLRDIDIHLINKRYFLLIQLLCHLCAMSSSCCNPFLYAWLHDRFRSELRKML--KC 346
[Close]
BLAST of GCR009 vs. Ensembl Medaka
Match: tacr3 (tachykinin receptor 3 [Source:NCBI gene;Acc:101159167])

HSP 1 Score: 144.821 bits (364), Expect = 2.772e-38
Identity = 104/364 (28.57%), Postives = 179/364 (49.18%), Query Frame = 1 
Query:  217 FGNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWNN-NNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMND----SFKSDFRWLVYHKCCGLCQRQSWSRKIN-------FNTTSARNSVSNNFSPALTIQNRKYNDKFPSRDHSSPERSKEIQFLFNKDNNECVNHNS 1272
            FGNV+VI   W+             FL NLA SD  ++ F     F Y    +W F   +C       + +V AS  +   I +DR+  +I+P+K R++ K +  +I CIW  +I+ +   C+ S   +R    T C   W    +    Y I + ++ Y LPL+V+ +TYTIVG+ LW   +PG+  +    + +++K KV+KM+ I+V+ F LCWLP H++F+VT +N+     K +   Y+   W+AMS++  NP++Y  +N      FK  FRW  + K     + +  S +++       +  T    ++   + PA   +          R HS P R +   ++ ++  +  +++NS
Sbjct:   41 FGNVIVI---WIILAHKRMRTVTNYFLLNLAFSDASMAAFNTLINFIYAAHGEWYFGEAYCKFHNFFPVTSVFASIYSMTAIAVDRYMAIIHPLKPRLSAKATTGIIVCIWSLAIVLAFPLCYFSTTQVRPRR-TICYVAWPRMADDSFMYHIIVAVLIYVLPLVVMGITYTIVGLTLWGGEIPGDTSDNYHGQ-LQAKRKVVKMMIIVVVTFALCWLPYHIYFIVTGINKRLSKWKYIQQVYLSVMWLAMSSTMYNPIIYCCLNSRFRAGFKRAFRWCPFIKVSSYDELELRSTRLHPTRQSSMYTLTRMDTTMVVVYDPA---EGDGGAGGGTGRKHSLPARKRS--YITSRQTDITISNNS 394
[Close]
BLAST of GCR009 vs. Planmine SMEST
Match: SMESG000035936.1 (SMESG000035936.1)

HSP 1 Score: 874.774 bits (2259), Expect = 0.000e+0
Identity = 442/442 (100.00%), Postives = 442/442 (100.00%), Query Frame = 1 
Query:   88 METTVANRFPNNSIKNGSDQIPWTFNEKVMXXXXXXXXXXXXXFGNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCCGLCQRQSWSRKINFNTTSARNSVSNNFSPALTIQNRKYNDKFPSRDHSSPERSKEIQFLFNKDNNECVNHNSIDLPREFQVKSKPLSTNMNKIKILILDAEVEKNRIFTNQNYQIITQL 1413
            METTVANRFPNNSIKNGSDQIPWTFNEKVMIIISYSTSISLSLFGNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCCGLCQRQSWSRKINFNTTSARNSVSNNFSPALTIQNRKYNDKFPSRDHSSPERSKEIQFLFNKDNNECVNHNSIDLPREFQVKSKPLSTNMNKIKILILDAEVEKNRIFTNQNYQIITQL
Sbjct:    1 METTVANRFPNNSIKNGSDQIPWTFNEKVMIIISYSTSISLSLFGNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDFRWLVYHKCCGLCQRQSWSRKINFNTTSARNSVSNNFSPALTIQNRKYNDKFPSRDHSSPERSKEIQFLFNKDNNECVNHNSIDLPREFQVKSKPLSTNMNKIKILILDAEVEKNRIFTNQNYQIITQL 442
[Close]
BLAST of GCR009 vs. Planmine SMEST
Match: SMESG000035936.1 (SMESG000035936.1)

HSP 1 Score: 866.3 bits (2237), Expect = 0.000e+0
Identity = 442/453 (97.57%), Postives = 442/453 (97.57%), Query Frame = 1 
Query:   88 METTVANRFPNNSIKNGSDQIPWTFNEKVMXXXXXXXXXXXXXFGNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFK-----------SDFRWLVYHKCCGLCQRQSWSRKINFNTTSARNSVSNNFSPALTIQNRKYNDKFPSRDHSSPERSKEIQFLFNKDNNECVNHNSIDLPREFQVKSKPLSTNMNKIKILILDAEVEKNRIFTNQNYQIITQL 1413
            METTVANRFPNNSIKNGSDQIPWTFNEKVMIIISYSTSISLSLFGNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFK           SDFRWLVYHKCCGLCQRQSWSRKINFNTTSARNSVSNNFSPALTIQNRKYNDKFPSRDHSSPERSKEIQFLFNKDNNECVNHNSIDLPREFQVKSKPLSTNMNKIKILILDAEVEKNRIFTNQNYQIITQL
Sbjct:    1 METTVANRFPNNSIKNGSDQIPWTFNEKVMIIISYSTSISLSLFGNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKELVYNFEHRIRSDFRWLVYHKCCGLCQRQSWSRKINFNTTSARNSVSNNFSPALTIQNRKYNDKFPSRDHSSPERSKEIQFLFNKDNNECVNHNSIDLPREFQVKSKPLSTNMNKIKILILDAEVEKNRIFTNQNYQIITQL 453
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BLAST of GCR009 vs. Planmine SMEST
Match: SMESG000016672.1 (SMESG000016672.1)

HSP 1 Score: 145.976 bits (367), Expect = 7.921e-40
Identity = 90/262 (34.35%), Postives = 147/262 (56.11%), Query Frame = 1 
Query:  247 WLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTF-TYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMNDSFKSDF 1029
            W+     NRN     FL NL+++D+L+ L C P T  +  LL+ WPF  L C VV  IQ ++V  S +T++VI  DRF VV +PMK+R++ + S  +IG  W+ SI+ S     V+  S        C ENW N +  + YT  +L+  YFLP+  +T TY  + +KLW R  PG +   R+ +   SK +++KM+ I+   + L  LP H+ F++ + N+    +K++    +  H++++S +  N  +Y ++N +FK  F
Sbjct:   40 WILIKDINRN-ATNIFLFNLSLADILMCLICSPVTLVSDGLLFYWPFGSLLCKVVPFIQGISVFLSGLTHVVISSDRFVVVFFPMKTRLSNECSWGLIGLTWIISIILSLPLVIVNEISTINQNRDLCEENWKNKDHGLIYTFVVLICQYFLPVAFITSTYIAIAVKLWIRKPPGVSFPGRNEQVNNSKKRMVKMVIIVASFYSLSQLPRHVLFLIGYSNELFFSSKHVKELLLFSHFLSISATTYNIFIYLWLNRNFKESF 300
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BLAST of GCR009 vs. Planmine SMEST
Match: SMESG000017198.1 (SMESG000017198.1)

HSP 1 Score: 147.132 bits (370), Expect = 1.191e-39
Identity = 93/274 (33.94%), Postives = 153/274 (55.84%), Query Frame = 1 
Query:  220 GNVVVIIVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTF-TYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSR-ITRKRSGLVIGCIWVSSILFSSVQCFVSR-ASIREGGYTECSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVV----CHWIAMSNSFVNPVVYSFMNDSFK 1020
            GN+ V    ++ +CR  R +    FL NL+++DL ++LFCVP TF +  +   W F    C V   +Q L+V  SS+T+  I +DR++VV +P++ +    K +  V+ CIW+ SI  S    FV+   +I E  +  CSE W   N ++ Y++ ++++ YFLP+IV+ +TY+ + I+LW    PGE+   RD     SK K+IKM  I+ I + L  LP H+ ++ +  + S       +   V      H ++MS  F NP++Y ++N SF+
Sbjct:   39 GNLAVC---YIISCR-LRQSATSIFLLNLSLADLKMALFCVPVTFFSDCIFMRWIFGRAMCHVFPFVQALSVFLSSVTHTAISVDRYFVVFHPLRRQNFPIKGAVFVVSCIWIFSISISFPLIFVTEYKTINESDF--CSEKWPTENNEMLYSLFVMVLQYFLPIIVIIMTYSAIAIRLWFYQTPGESIRSRDEVIATSKKKMIKMFIIVAIFYSLSQLPRHIIYLTSKFSVSTSQNPFWDSTTVTILNFAHLLSMSACFYNPILYVWLNSSFR 306
[Close]
BLAST of GCR009 vs. Planmine SMEST
Match: SMESG000023192.1 (SMESG000023192.1)

HSP 1 Score: 143.28 bits (360), Expect = 3.470e-38
Identity = 92/323 (28.48%), Postives = 168/323 (52.01%), Query Frame = 1 
Query:  238 IVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFPYLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVIGCIWVSSILFSSVQCFVSRASIREGGYTECSENWNNNNLQISYTISILLITYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSIIVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNPVVYSFMND----SFKSDFRWLVYHKCCGLCQRQSWS-RKINFNTTSAR--------NSVSNNFSPALTIQNRK 1167
            I+ W+   + +  N    FL NL+ +++L+++  +     Y +   W F    C +   I   +VT + +T + +  DRF V++YP+++R++ K   L+I  IW+ +I    +    ++  + E    +  +    +  ++ Y IS+L++ Y LPL+VLT+TY  + I+LWK +  GE +     + MK+K K++KML  I+I F +CWLP   FF++  +N+    + N+ I + + + +AMS SF NP++Y  M+      FKS FRW         C   +W   + +  T + R        N +++N +  L ++N K
Sbjct:   46 IMVWIILSQKSMRNSTNYFLVNLSATNVLMAVTHIFPNVYYSINNKWIFGLTLCKLSQFITAFSVTVTVLTFIGMAADRFTVIMYPLRTRLSSKVILLIIVVIWLIAIASGLIGAIKAKIVLDENR--KICDFLTEDEWKLKYDISLLILQYILPLVVLTITYIPISIRLWKSTTVGELNR-SQRECMKTKRKIVKMLIAIIIIFAVCWLPYQCFFIIIDLNKHLNASPNVLIVFFIIYALAMSYSFTNPLIYCIMSKKFRMGFKSFFRW---------CPFVTWPLAQTDLKTQTNRSCDYFKGNNDLTSNGTERLLVENEK 356
The following BLAST results are available for this feature:
BLAST of GCR009 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TACR32.213e-4429.13tachykinin receptor 3 [Source:HGNC Symbol;Acc:HGNC... [more]
TACR24.502e-4329.08tachykinin receptor 2 [Source:HGNC Symbol;Acc:HGNC... [more]
TACR21.531e-4229.84tachykinin receptor 2 [Source:HGNC Symbol;Acc:HGNC... [more]
NPY2R7.064e-3835.57neuropeptide Y receptor Y2 [Source:HGNC Symbol;Acc... [more]
NPY2R7.064e-3835.57neuropeptide Y receptor Y2 [Source:HGNC Symbol;Acc... [more]
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BLAST of GCR009 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
npr-221.101e-3632.30Neuropeptide receptor 22 [Source:UniProtKB/Swiss-... [more]
npr-221.118e-3632.30Neuropeptide receptor 22 [Source:UniProtKB/Swiss-... [more]
tkr-31.294e-3126.10TachyKinin Receptor family [Source:UniProtKB/TrEM... [more]
tkr-31.781e-3125.41TachyKinin Receptor family [Source:UniProtKB/TrEM... [more]
tkr-28.807e-2728.72TachyKinin Receptor family [Source:UniProtKB/TrEM... [more]
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BLAST of GCR009 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TkR99D9.598e-4730.40gene:FBgn0004622 transcript:FBtr0085507[more]
TkR99D2.364e-4631.63gene:FBgn0004622 transcript:FBtr0301656[more]
TkR99D2.597e-4630.40gene:FBgn0004622 transcript:FBtr0330298[more]
Lkr2.641e-4431.64gene:FBgn0035610 transcript:FBtr0077149[more]
TkR86C2.242e-4327.54gene:FBgn0004841 transcript:FBtr0082315[more]
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BLAST of GCR009 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
npffr1l21.704e-4233.43neuropeptide FF receptor 1 like 2 [Source:ZFIN;Acc... [more]
CU915819.21.812e-4231.53neuropeptide FF receptor 2a [Source:NCBI gene;Acc:... [more]
AL929374.11.812e-4231.53neuropeptide FF receptor 2a [Source:NCBI gene;Acc:... [more]
NPFFR21.812e-4231.53neuropeptide FF receptor 2a [Source:NCBI gene;Acc:... [more]
NPFFR21.812e-4231.53neuropeptide FF receptor 2a [Source:NCBI gene;Acc:... [more]
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BLAST of GCR009 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
NPY2R5.425e-4034.97neuropeptide Y receptor Y2 [Source:NCBI gene;Acc:1... [more]
errfi11.261e-3929.35ERBB receptor feedback inhibitor 1 [Source:Xenbase... [more]
ENSXETT00000044281.14.049e-3930.80pep primary_assembly:Xenopus_tropicalis_v9.1:3:115... [more]
taf1b7.509e-3931.25TATA-box binding protein associated factor, RNA po... [more]
clstn21.880e-3630.30calsyntenin 2 [Source:Xenbase;Acc:XB-GENE-958942][more]
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BLAST of GCR009 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tacr22.885e-4630.14tachykinin receptor 2 [Source:MGI Symbol;Acc:MGI:9... [more]
Tacr37.166e-3932.97tachykinin receptor 3 [Source:MGI Symbol;Acc:MGI:8... [more]
Npy2r1.069e-3735.04neuropeptide Y receptor Y2 [Source:MGI Symbol;Acc:... [more]
Npy2r1.069e-3735.04neuropeptide Y receptor Y2 [Source:MGI Symbol;Acc:... [more]
Npy2r1.069e-3735.04neuropeptide Y receptor Y2 [Source:MGI Symbol;Acc:... [more]
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BLAST of GCR009 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P30975|TLR2_DROME9.621e-4630.40Tachykinin-like peptides receptor 99D OS=Drosophil... [more]
sp|P30549|NK2R_MOUSE2.018e-4530.14Substance-K receptor OS=Mus musculus OX=10090 GN=T... [more]
sp|P16610|NK2R_RAT3.019e-4529.41Substance-K receptor OS=Rattus norvegicus OX=10116... [more]
sp|P51144|NK2R_MESAU3.020e-4529.41Substance-K receptor OS=Mesocricetus auratus OX=10... [more]
sp|P79218|NK2R_RABIT3.280e-4530.14Substance-K receptor OS=Oryctolagus cuniculus OX=9... [more]
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BLAST of GCR009 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A193KUF70.000e+0100.00GCR009 OS=Schmidtea mediterranea OX=79327 GN=gcr00... [more]
A0A210PF756.819e-8342.74Neuropeptide FF receptor 2 OS=Mizuhopecten yessoen... [more]
V4BK897.573e-8344.95G_PROTEIN_RECEP_F1_2 domain-containing protein (Fr... [more]
R7TCU38.420e-8350.74G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A0K0PUK81.936e-8041.30FMRFamide receptor 1 OS=Platynereis dumerilii OX=6... [more]
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BLAST of GCR009 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
NPFFR27.277e-4333.67neuropeptide FF receptor 2 [Source:NCBI gene;Acc:1... [more]
npffr1l37.131e-4030.96neuropeptide FF receptor 1-like [Source:NCBI gene;... [more]
npffr2b1.284e-3932.54neuropeptide FF receptor 2-like [Source:NCBI gene;... [more]
npy2rl1.974e-3833.33neuropeptide Y receptor type 2-like [Source:NCBI g... [more]
npy2rl1.974e-3833.33neuropeptide Y receptor type 2-like [Source:NCBI g... [more]
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BLAST of GCR009 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
prlh2r1.643e-3931.27prolactin releasing hormone 2 receptor [Source:ZFI... [more]
ENSPMAT00000008040.16.673e-3731.27pep scaffold:Pmarinus_7.0:GL477351:476361:492361:-... [more]
qrfpr43.290e-3430.10pyroglutamylated RFamide peptide receptor 4 [Sourc... [more]
ENSPMAT00000010661.14.630e-3439.42pep scaffold:Pmarinus_7.0:GL479584:296:6514:-1 gen... [more]
npffr1l38.122e-3330.15neuropeptide FF receptor 1 like 3 [Source:ZFIN;Acc... [more]
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BLAST of GCR009 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR009 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO397197.499e-3429.79Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO475351.159e-3330.24Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO444182.817e-3329.35Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO397155.603e-3331.66Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO495703.020e-3230.71Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of GCR009 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000042596.11.759e-3929.47neuropeptide FF receptor 2 [Source:NCBI gene;Acc:1... [more]
npffr1l21.779e-3928.26neuropeptide FF receptor 1 like 2 [Source:ZFIN;Acc... [more]
npffr1l21.961e-3928.26neuropeptide FF receptor 1 like 2 [Source:ZFIN;Acc... [more]
prlhr2b1.316e-3832.34prolactin-releasing peptide receptor-like [Source:... [more]
tacr32.772e-3828.57tachykinin receptor 3 [Source:NCBI gene;Acc:101159... [more]
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BLAST of GCR009 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000035936.10.000e+0100.00SMESG000035936.1[more]
SMESG000035936.10.000e+097.57SMESG000035936.1[more]
SMESG000016672.17.921e-4034.35SMESG000016672.1[more]
SMESG000017198.11.191e-3933.94SMESG000017198.1[more]
SMESG000023192.13.470e-3828.48SMESG000023192.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031677 ID=SMED30031677|Name=GCR009|organism=Schmidtea mediterranea sexual|type=transcript|length=1434bp
ATATTTTACAAGATTTATAATGGATTCGGGACCAAAATCTGAAATTAAAA
ATGTGTTTTACATGGCTATTTCATATCAAATTGAATAATGGAAACAACAG
TGGCAAACAGATTTCCAAACAATTCTATTAAAAATGGTTCAGATCAAATT
CCGTGGACTTTCAATGAAAAGGTGATGATAATTATTTCTTATTCAACTTC
TATCAGTTTATCTCTTTTTGGAAATGTCGTCGTGATTATTGTTTTTTGGC
TTGGAACATGTCGACCCAATCGCAATAATGAAGTTCGACAGTTCTTGACA
AATCTTGCTGTGTCAGATTTATTAATAAGCTTATTTTGCGTTCCATTCAC
CTTTACTTATATATTATTATGGGACTGGCCATTTCCATATTTATTCTGTC
CAGTTGTTCTTTCTATCCAATTACTAGCCGTAACCGCTTCTTCAATAACG
AACATGGTGATTGGTTTAGATAGATTTTGGGTCGTCATTTATCCAATGAA
ATCCCGTATTACCAGGAAAAGATCTGGATTGGTAATTGGATGTATTTGGG
TGAGCTCAATCCTGTTTTCTTCGGTTCAATGTTTCGTAAGTCGAGCATCC
ATTAGAGAAGGTGGATACACCGAATGCTCAGAAAATTGGAATAATAATAA
TCTACAAATTTCATATACGATCTCAATACTTTTAATAACATATTTTCTAC
CATTAATCGTTCTCACTGTGACATATACAATTGTTGGGATAAAACTTTGG
AAGCGATCCTTACCAGGAGAAGCTGATGAAATACGGGACGCAAAACATAT
GAAATCAAAAGTCAAAGTAATAAAAATGTTATCAATAATTGTCATATGCT
TTGGATTGTGTTGGCTTCCTCTGCATTTATTCTTCGTTGTCACCCATGTG
AACCAGTCTCAGGGATACACAAAGAATCTCAATATCGCGTATGTCGTTTG
CCATTGGATCGCAATGTCGAACAGCTTCGTCAACCCGGTAGTCTACAGCT
TCATGAATGATTCATTTAAATCTGATTTTCGATGGCTTGTCTATCACAAA
TGTTGTGGACTTTGTCAACGACAATCCTGGTCAAGAAAGATCAACTTCAA
CACAACGAGTGCTCGAAATAGTGTTTCTAATAACTTTTCTCCAGCACTAA
CGATCCAAAACAGAAAATACAACGACAAATTCCCGTCTCGAGATCACTCA
TCACCAGAAAGGTCCAAGGAGATTCAATTCCTGTTCAACAAAGACAACAA
CGAATGTGTGAACCATAATTCAATAGACTTACCGAGAGAGTTCCAAGTTA
AATCAAAACCCTTGTCAACAAATATGAACAAAATAAAAATTCTCATTCTT
GATGCCGAAGTTGAGAAGAATCGAATATTTACCAACCAAAACTACCAAAT
AATTACTCAACTCTAAATGTCAGTGTCATTTGTG
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protein sequence of SMED30031677-orf-1

>SMED30031677-orf-1 ID=SMED30031677-orf-1|Name=SMED30031677-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=443bp
METTVANRFPNNSIKNGSDQIPWTFNEKVMIIISYSTSISLSLFGNVVVI
IVFWLGTCRPNRNNEVRQFLTNLAVSDLLISLFCVPFTFTYILLWDWPFP
YLFCPVVLSIQLLAVTASSITNMVIGLDRFWVVIYPMKSRITRKRSGLVI
GCIWVSSILFSSVQCFVSRASIREGGYTECSENWNNNNLQISYTISILLI
TYFLPLIVLTVTYTIVGIKLWKRSLPGEADEIRDAKHMKSKVKVIKMLSI
IVICFGLCWLPLHLFFVVTHVNQSQGYTKNLNIAYVVCHWIAMSNSFVNP
VVYSFMNDSFKSDFRWLVYHKCCGLCQRQSWSRKINFNTTSARNSVSNNF
SPALTIQNRKYNDKFPSRDHSSPERSKEIQFLFNKDNNECVNHNSIDLPR
EFQVKSKPLSTNMNKIKILILDAEVEKNRIFTNQNYQIITQL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000140anterior region of the whole animal
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR000611NPY_rcpt
IPR017452GPCR_Rhodpsn_7TM
IPR000276GPCR_Rhodpsn
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
GO:0007165signal transduction
GO:0007218neuropeptide signaling pathway
Vocabulary: molecular function
TermDefinition
GO:0004983neuropeptide Y receptor activity
GO:0004930G-protein coupled receptor activity
GO:0004871signal transducer activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000276G protein-coupled receptor, rhodopsin-likePRINTSPR00237GPCRRHODOPSNcoord: 194..217
score: 22.13
coord: 244..268
score: 31.99
coord: 66..87
score: 34.96
coord: 285..311
score: 30.14
coord: 145..166
score: 22.18
coord: 30..54
score: 23.35
coord: 111..133
score: 24.44
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 45..303
e-value: 3.2E-47
score: 161.1
IPR000276G protein-coupled receptor, rhodopsin-likePROSITEPS00237G_PROTEIN_RECEP_F1_1coord: 117..133
IPR000611Neuropeptide Y receptor familyPRINTSPR01012NRPEPTIDEYRcoord: 111..126
score: 31.53
coord: 301..314
score: 35.39
coord: 88..100
score: 40.56
coord: 290..299
score: 37.27
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 3..358
e-value: 5.6E-85
score: 286.9
NoneNo IPR availablePANTHERPTHR24235FAMILY NOT NAMEDcoord: 7..349
NoneNo IPR availableCDDcd153917tmA_NPR-like_invertebratecoord: 45..313
e-value: 3.48274E-96
score: 289.03
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 21..333
NoneNo IPR availableTMHMMTMhelixcoord: 73..95
NoneNo IPR availableTMHMMTMhelixcoord: 246..268
NoneNo IPR availableTMHMMTMhelixcoord: 283..305
NoneNo IPR availableTMHMMTMhelixcoord: 191..213
NoneNo IPR availableTMHMMTMhelixcoord: 31..53
NoneNo IPR availableTMHMMTMhelixcoord: 148..167
NoneNo IPR availableTMHMMTMhelixcoord: 105..127
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 45..303
score: 41.455