DNA polymerase epsilon subunit

Overview
NameDNA polymerase epsilon subunit
Smed IDSMED30031663
Length (bp)1808
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DNA polymerase epsilon subunit (SMED30031663) t-SNE clustered cells

Violin plots show distribution of expression levels for DNA polymerase epsilon subunit (SMED30031663) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DNA polymerase epsilon subunit (SMED30031663) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DNA polymerase epsilon subunit (SMED30031663) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30031663

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30031663h1SMcG0002348 SmedASXL_013111SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30031663h1SMcG0002348 SmedASXL_013111SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30031663h1SMcG0002348 dd_Smed_v6_16802_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
zeta neoblastSMED30031663h1SMcG0002348 dd_Smed_v4_16802_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30031663h1SMcG0002348 SMED_09864_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30031663h1SMcG0002348 SMED_09864_V2_1GPL14150PMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of DNA polymerase epsilon subunit vs. Ensembl Human
Match: POLE2 (DNA polymerase epsilon 2, accessory subunit [Source:HGNC Symbol;Acc:HGNC:9178])

HSP 1 Score: 264.233 bits (674), Expect = 2.685e-80
Identity = 179/570 (31.40%), Postives = 294/570 (51.58%), Query Frame = 3 
Query:  126 FKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKDTNSL--KLFQVIDAFHIPKLVFDCNSKKYIIDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFN-----YHN-------SLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRI---IFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYPFTGQVQN 1784
            FKL G LL  +A+ Y+TE + +    +    ++K+I+      L +N I   +++  +  C +  +     +F +I AF IP+ V++   KK++   +++       LF    + K+++ R RY I++QR  R + F       H         LK I  +L + + I + I+LGM++Q  EG++FLED TG V+++L  A++H GL  E  FVLAEG +E+++ +V+  G P  EP++ +R A   NIN+FGG P    VK+   +  L + E  D   VFLSD++LDQ  V++KL+I+F GY    P  FILCG+F S +  G    ++++ LK+ ++ +      +PD+   S  +FVPGP D   G     P               ++S  +  + R+   +FT+NPCR+ +  +EI +FRE ++ K+ R+C+  P+                    N  I N                  + + TILSQ HL PLP+++ PVYW +D AL ++P+PDLLV++D          + C  +NP +F   G F F ++YP    V++
Sbjct:   14 FKLRGLLLRGEAIKYLTEALQSISELELEDKLEKIINAVEKQPLSSNMIERSVVEAAVQECSQSVDETIEHVFNIIGAFDIPRFVYNSERKKFLPLLMTNHPA--PNLFGTPRD-KAEMFRERYTILHQRTHRHELFTPPVIGSHPDESGSKFQLKTIETLLGSTTKIGDAIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLYTEACFVLAEGWFEDQVFHVNAFGFPPTEPSSTTR-AYYGNINFFGG-PSNTSVKTSAKLKQL-EEENKDAMFVFLSDVWLDQVEVLEKLRIMFAGYSPAPPTCFILCGNF-SSAPYG---KNQVQALKDSLKTLADIICEYPDIHQSSRFVFVPGPEDPGFGSILPRPPL-------------AESITNEFRQRVPFSVFTTNPCRIQYCTQEITVFREDLVNKMCRNCVRFPSS-------------------NLAIPN------------------HFVKTILSQGHLTPLPLYVCPVYWAYDYALRVYPVPDLLVIADKYDPFTTTN-TECLCINPGSFPRSG-FSFKVFYPSNKTVED 521
[Close]
BLAST of DNA polymerase epsilon subunit vs. Ensembl Human
Match: POLE2 (DNA polymerase epsilon 2, accessory subunit [Source:HGNC Symbol;Acc:HGNC:9178])

HSP 1 Score: 259.225 bits (661), Expect = 9.247e-79
Identity = 162/516 (31.40%), Postives = 265/516 (51.36%), Query Frame = 3 
Query:  126 FKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKDTNSL--KLFQVIDAFHIPKLVFDCNSKKYIIDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFN-----YHN-------SLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRI---IFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNAL 1622
            FKL G LL  +A+ Y+TE + +    +    ++K+I+      L +N I   +++  +  C +  +     +F +I AF IP+ V++   KK++   +++       LF    + K+++ R RY I++QR  R + F       H         LK I  +L + + I + I+LGM++Q  EG++FLED TG V+++L  A++H GL  E  FVLAEG +E+++ +V+  G P  EP++ +R A   NIN+FGG P    VK+   +  L + E  D   VFLSD++LDQ  V++KL+I+F GY    P  FILCG+F S +  G    ++++ LK+ ++ +      +PD+   S  +FVPGP D   G     P               ++S  +  + R+   +FT+NPCR+ +  +EI +FRE ++ K+ R+C+  P+                    N  I N                  + + TILSQ HL PLP+++ PVYW +D AL
Sbjct:   14 FKLRGLLLRGEAIKYLTEALQSISELELEDKLEKIINAVEKQPLSSNMIERSVVEAAVQECSQSVDETIEHVFNIIGAFDIPRFVYNSERKKFLPLLMTNHPA--PNLFGTPRD-KAEMFRERYTILHQRTHRHELFTPPVIGSHPDESGSKFQLKTIETLLGSTTKIGDAIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLYTEACFVLAEGWFEDQVFHVNAFGFPPTEPSSTTR-AYYGNINFFGG-PSNTSVKTSAKLKQL-EEENKDAMFVFLSDVWLDQVEVLEKLRIMFAGYSPAPPTCFILCGNF-SSAPYG---KNQVQALKDSLKTLADIICEYPDIHQSSRFVFVPGPEDPGFGSILPRPPL-------------AESITNEFRQRVPFSVFTTNPCRIQYCTQEITVFREDLVNKMCRNCVRFPSS-------------------NLAIPN------------------HFVKTILSQGHLTPLPLYVCPVYWAYDYAL 469
[Close]
BLAST of DNA polymerase epsilon subunit vs. Ensembl Human
Match: POLE2 (DNA polymerase epsilon 2, accessory subunit [Source:HGNC Symbol;Acc:HGNC:9178])

HSP 1 Score: 241.506 bits (615), Expect = 4.948e-72
Identity = 172/568 (30.28%), Postives = 278/568 (48.94%), Query Frame = 3 
Query:  126 FKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKDTNSLKLFQVIDAFHIPKLVFDCNSKKYIIDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFN-----YHN-------SLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRI---IFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYPFTGQVQN 1784
            FKL G LL  +A+ Y+TE + +    +    ++K+I+      L +N I   +++  +  C +  +   L                          +   P    LF    + K+++ R RY I++QR  R + F       H         LK I  +L + + I + I+LGM++Q  EG++FLED TG V+++L  A++H GL  E  FVLAEG +E+++ +V+  G P  EP++ +R A   NIN+FGG P    VK+   +  L + E  D   VFLSD++LDQ  V++KL+I+F GY    P  FILCG+F S +  G    ++++ LK+ ++ +      +PD+   S  +FVPGP D   G     P               ++S  +  + R+   +FT+NPCR+ +  +EI +FRE ++ K+ R+C+  P+                    N  I N                  + + TILSQ HL PLP+++ PVYW +D AL ++P+PDLLV++D          + C  +NP +F   G F F ++YP    V++
Sbjct:   14 FKLRGLLLRGEAIKYLTEALQSISELELEDKLEKIINAVEKQPLSSNMIERSVVEAAVQECSQSVDETILLMT-----------------------NHPAP---NLFGTPRD-KAEMFRERYTILHQRTHRHELFTPPVIGSHPDESGSKFQLKTIETLLGSTTKIGDAIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLYTEACFVLAEGWFEDQVFHVNAFGFPPTEPSSTTR-AYYGNINFFGG-PSNTSVKTSAKLKQL-EEENKDAMFVFLSDVWLDQVEVLEKLRIMFAGYSPAPPTCFILCGNF-SSAPYG---KNQVQALKDSLKTLADIICEYPDIHQSSRFVFVPGPEDPGFGSILPRPPL-------------AESITNEFRQRVPFSVFTTNPCRIQYCTQEITVFREDLVNKMCRNCVRFPSS-------------------NLAIPN------------------HFVKTILSQGHLTPLPLYVCPVYWAYDYALRVYPVPDLLVIADKYDPFTTTN-TECLCINPGSFPRSG-FSFKVFYPSNKTVED 495
[Close]
BLAST of DNA polymerase epsilon subunit vs. Ensembl Celegans
Match: pole-2 (Probable DNA polymerase epsilon subunit 2 [Source:UniProtKB/Swiss-Prot;Acc:Q19196])

HSP 1 Score: 188.348 bits (477), Expect = 1.843e-52
Identity = 168/564 (29.79%), Postives = 268/564 (47.52%), Query Frame = 3 
Query:  126 FKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKD--TNSLKLFQVIDAFHIPKLVFDCNSKKYIIDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSF-NYHNSLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINL-DNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRIIFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCN--VLNPFNFSTEGDFPFNIYYPFTGQVQNCNVNV 1799
            F++H F L+ +AV    ++  + D     K  +KMI       L ++ IS E+++ T   C+     ++ KL  V DAF +    FD + +K ++ +  ++      L +DSS   S   R R+ I+ QR MR  S  N+  +   I    ++   +++V++LGML+Q     + +ED +G VE+   ++ ++H  L  E S  + EG +E  +L V+ + M  VE A  +R  +  N N+FGG        S +  + L + E  D +LVFLSD+FLD + V++ +  L QGYK   P A + CG+F S        +D + LL  G R +             +  IFVPGP+D F       PH           +F   S I S      F SNPCR+ F  +EIV+FR  +++K  RH                       SI + T+++               +      ++LSQAHL PLP HI+PV   F ++L L PLPDLL+ +D   +    ++ G +  V NP +FS   ++ F++YYP   +V+   + V
Sbjct:   18 FQIHAFELKKEAVKLCVKLFLDHDKETRKKWTNKMIELLKKQTLQSSLISEELIRDTFRQCKSKGTQDAGKLLNVFDAFSLQPYDFDADLRKMVLRKEKTS------LAADSSSF-SHAARQRFFIVKQRAMRCASLKNFKFTTCEILG--SSTKHLQSVVVLGMLTQQKADCYHIEDLSGSVEVEFKEDTKFHHALFHEHSIAIFEGTFENSVLTVNEVAMVPVESAEVTRKELSSNENWFGGDDKIAFRCSDRLRSALAKQE--DTSLVFLSDVFLDDKKVMKAVFKLLQGYKDQPPVAIVFCGNFCSRPR----QTDTIDLLDRGFRWLANQLTPLRKDYEKTQFIFVPGPDDPFVDTVLPRPHLPSL-------LFKHISPIIS----CTFASNPCRIQFASQEIVVFRSDLIKKTCRH-----------------------SINSITVES---------------IPSRYARSVLSQAHLCPLPQHITPVLPDFSHSLSLHPLPDLLITADRFETFTE-KVVGADTIVSNPGSFS-RSNYTFHVYYPCQNRVEASQIPV 515
[Close]
BLAST of DNA polymerase epsilon subunit vs. Ensembl Fly
Match: DNApol-epsilon58 (gene:FBgn0035644 transcript:FBtr0077081)

HSP 1 Score: 188.734 bits (478), Expect = 1.650e-52
Identity = 162/587 (27.60%), Postives = 271/587 (46.17%), Query Frame = 3 
Query:  105 KENLKNYFKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKDT--NSLKLFQVIDAFHIPKLVFDCNSKKYIIDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFN--------------YHNSLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGG-----LPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDIS---DKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRIIFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYPFTGQVQNCNV 1793
            ++ + N FKL GFL+  +  +Y+ E +  FD+A+  K +  +        L    +    L++ IN   +        +F +IDAF +P+  ++   KK+ +D     +P  +QL + +  +KS  ++ RY ++ Q+ +R   F                   L+   N+L T SA++  ++LG+L+Q  EG++++ED TG V+++L  A +H G   EG FVLAEG Y   +L VD +G P  EPA +SR A     N +GG     L    G++  +  N        +  +VFLSD+ LD  +V++KL+ LF GY    P+A +L G F + +    ++    D L  L  G   + K           ++LI VP   D     +  +   +     L  G+  +         R    +NPCR+ +  ++IV+ R  +M K  R+ ++ P +  Q+E+                                     +   TI+ Q HL+P+     PV+W +D AL L+PLPDL+V+ DS  S  + Q  GC VLN  +F  +  F F +Y P T  +++  +
Sbjct:    9 RKRITNTFKLCGFLIRSENSSYLAEQLLPFDAAERDKWLTVITENLQSQKLLTPHVERAALEKAINELNRVGLDEGETVFALIDAFTVPRFRYNQRIKKFELD----TQP--RQLLT-APRMKSDYMQQRYAMLLQKTLRHDLFAPAVIQDGVGAEAQAKKFKLQFAENLLAT-SAMKEAVVLGLLTQLKEGKFYVEDPTGCVQLDLTGARFHAGFFCEGCFVLAEGNYNNGVLKVDGLGFPPAEPANSSR-AFFGTANTWGGESAKLLKYSAGLQELERTNT-------ETTIVFLSDVRLDLPVVMEKLRQLFVGYDSCPPQAIVLMGPFTASTRNHHELRHHLDALGGLAAGCEQLKKQ----------TDLILVPSSED---PTAPNILPRAPIPECLAAGLLKAWP-------RTQLATNPCRLQYCTQQIVVCRLDLMAKFCRNTLHFPEDTSQIEQ-------------------------------------HFARTIVCQGHLVPIHPIAMPVHWDYDPALWLYPLPDLIVMGDSCQSFSSSQ-HGCTVLNTGSF-VKSKFAFKVYIPATRTIEDSEI 520
[Close]
BLAST of DNA polymerase epsilon subunit vs. Ensembl Zebrafish
Match: pole2 (polymerase (DNA directed), epsilon 2 [Source:ZFIN;Acc:ZDB-GENE-020419-16])

HSP 1 Score: 166.392 bits (420), Expect = 4.175e-46
Identity = 115/389 (29.56%), Postives = 188/389 (48.33%), Query Frame = 3 
Query:  630 GRWFLEDETGYVEINLDNAE-YHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRI---IFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYPFTGQVQN 1784
            G+++LED +G V++++  A  +H GL  E  FVLAEG YE+ + +V   G P  EP++ +R A   N+N+FGG P    VK+   +  L + E  D   V +SD++LD   V++K+  +F GY    P  FI CG F S +  G      L++L + IR ++  ++        S  +FVPGP D   G     P               ++   +  + R+   +FT+NPCR+ +  +E+VI RE ++ K+ R+C  +P+             SLD                         +  + + T+LSQ HL PLP+++ PV+W +D+ L        +++       FNI  + C  +NP +F   G F F +YYP +  V++
Sbjct:    1 GKFYLEDPSGTVQLDISKAPLFHSGLYTESCFVLAEGWYEDSVFHVSAFGFPPTEPSSFTR-AYFGNVNFFGG-PSSTAVKASAKLKQL-EEENEDAMFVLVSDVWLDSVEVLEKIHTMFSGYSALPPTCFIFCGSF-SSAPYGRTQLRSLRVLLD-IRFILVFYS--------SRFVFVPGPEDPGPGAVLPRPPL-------------AEHITEEFQQRVPLSVFTTNPCRIQYCSQELVIIREDLVNKMCRNCERLPS------------SSLD-------------------------IPTHFVKTVLSQGHLTPLPLYVCPVFWAYDHTLR-LYPVPDVIVFADKYDPFNICNTDCLCINPGSFPRSG-FSFKVYYPSSRTVED 324
[Close]
BLAST of DNA polymerase epsilon subunit vs. Ensembl Xenopus
Match: pole2 (polymerase (DNA directed), epsilon 2, accessory subunit [Source:NCBI gene;Acc:595029])

HSP 1 Score: 263.462 bits (672), Expect = 9.271e-80
Identity = 181/570 (31.75%), Postives = 283/570 (49.65%), Query Frame = 3 
Query:  105 KENLKNYFKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRK--DTNSLKLFQVIDAFHIPKLVFDCNSKKYIIDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFN-----YHN-------SLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRI---IFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYP 1763
            K  + + FK+HG LL  +A  Y++EV+S+ +  +   V+D +I       L +N I    ++  +  C +  D     +F +I AF IP+ V++   KK++  ++++       LF  S+  K+++ R RY I+ QR  R + F       H         LK +  +L   + +  VI+LGM++Q  EG++FLED TG V+++L NA++H GL  E  FVLAEG YE+K+ +++  G P  EP++ +R A   N+N+FGG P    VK+   +  L + E  D   VFLSD++LDQ  V +KL  +F GY    P  FI CG+F S +  G    + ++ LK+  + +      +P +   S  +FVPGP D   G         L    + D I       +  + R+   IFT+NPCR+ +  +EIV+FRE ++ K+ R+C+  PN            ++LD                         +  + + TILSQ  L PLP  +SPVYW +D AL        LV+       F I  + C  +NP +F   G F F +YYP
Sbjct:   42 KSKVTSAFKMHGLLLRAEASKYLSEVLSSVNEVELEDVIDSIIDAVEKQPLSSNMIEKSTVETAVQECSRSCDETIEHIFNIIGAFDIPRYVYNSERKKFLPLKMTTLPS--PNLFG-SARDKAELFRERYTILQQRTHRHELFTPPVIGAHPDESRSKFQLKTVETLLGNTAKVGEVIVLGMITQLKEGKFFLEDPTGAVQLDLTNAQFHSGLYTESCFVLAEGWYEDKIFHINAFGFPPTEPSSTTR-AYYGNMNFFGG-PSSTSVKTSVKLRQL-EEENEDAMFVFLSDLWLDQVEVTEKLHTMFSGYSTAPPTCFIFCGNF-SSAPYG---KNHIRSLKDSFKILADIICEYPHIHKSSRFVFVPGPEDPGPGF-------ILPRPPIADHI------TEEFRQRVPFSIFTTNPCRIQYCTQEIVVFREDLVNKMCRNCVRFPN------------NTLD-------------------------IPNHFVKTILSQGLLTPLPPFVSPVYWAYDYALR-LYPLPDLVVIADKYDPFTITNTECLTINPGSFPRSG-FSFKVYYP 549
[Close]
BLAST of DNA polymerase epsilon subunit vs. Ensembl Xenopus
Match: pole2 (polymerase (DNA directed), epsilon 2, accessory subunit [Source:NCBI gene;Acc:595029])

HSP 1 Score: 251.521 bits (641), Expect = 4.096e-75
Identity = 179/570 (31.40%), Postives = 280/570 (49.12%), Query Frame = 3 
Query:  105 KENLKNYFKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKDTNSL--KLFQVIDAFHIPKLVFDCNSKKYIIDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFN-----YHN-------SLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRI---IFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYP 1763
            K  + + FK+HG LL  +A  Y++EV+S+ +  +   V+D +I       L +N I    ++  +  C +  +     +F +I AF IP+ V++   KK++  ++++       LF  S+  K+++ R RY I+ QR  R + F       H         LK +  +L   + +  VI+LGM++Q  EG++FLED TG V+++L N ++H GL  E  FVLAEG YE+K+ +++  G P  EP++ +R A   N+N+FGG P    VK+   +  L + E  D   VFLSD++LDQ  V +KL  +F GY    P  FI CG+F S +  G    + ++ LK    +++     F      S  +FVPGP D   G         L    + D I       +  + R+   IFT+NPCR+ +  +EIV+FRE ++ K+ R+C+  PN            ++LD                         +  + + TILSQ  L PLP  +SPVYW +D AL        LV+       F I  + C  +NP +F   G F F +YYP
Sbjct:   54 KSKVTSAFKMHGLLLRAEASKYLSEVLSSVNEVELEDVIDSIIDAVEKQPLSSNMIEKSTVETAVQECSRSCDETIEHIFNIIGAFDIPRYVYNSERKKFLPLKMTTLPS--PNLFG-SARDKAELFRERYTILQQRTHRHELFTPPVIGAHPDESRSKFQLKTVETLLGNTAKVGEVIVLGMITQLKEGKFFLEDPTGAVQLDLTNHQFHSGLYTESCFVLAEGWYEDKIFHINAFGFPPTEPSSTTR-AYYGNMNFFGG-PSSTSVKTSVKLRQL-EEENEDAMFVFLSDLWLDQVEVTEKLHTMFSGYSTAPPTCFIFCGNF-SSAPYG---KNHIRSLKANSFHLILIHICF--CVNSSRFVFVPGPEDPGPGF-------ILPRPPIADHI------TEEFRQRVPFSIFTTNPCRIQYCTQEIVVFREDLVNKMCRNCVRFPN------------NTLD-------------------------IPNHFVKTILSQGLLTPLPPFVSPVYWAYDYALR-LYPLPDLVVIADKYDPFTITNTECLTINPGSFPRSG-FSFKVYYP 559
[Close]
BLAST of DNA polymerase epsilon subunit vs. Ensembl Xenopus
Match: pole2 (polymerase (DNA directed), epsilon 2, accessory subunit [Source:NCBI gene;Acc:595029])

HSP 1 Score: 247.284 bits (630), Expect = 4.868e-74
Identity = 173/553 (31.28%), Postives = 272/553 (49.19%), Query Frame = 3 
Query:  156 KAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKDTNSL--KLFQVIDAFHIPKLVFDCNSKKYIIDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFN-----YHN-------SLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRI---IFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYP 1763
            KA  Y++EV+S+ +  +   V+D +I       L +N I    ++  +  C +  +     +F +I AF IP+ V++   KK++  ++++       LF  S+  K+++ R RY I+ QR  R + F       H         LK +  +L   + +  VI+LGM++Q  EG++FLED TG V+++L N ++H GL  E  FVLAEG YE+K+ +++  G P  EP++ +R A   N+N+FGG P    VK+   +  L + E  D   VFLSD++LDQ  V +KL  +F GY    P  FI CG+F S +  G    + ++ LK+  + +      +P +   S  +FVPGP D   G         L    + D I       +  + R+   IFT+NPCR+ +  +EIV+FRE ++ K+ R+C+  PN            ++LD                         +  + + TILSQ  L PLP  +SPVYW +D AL        LV+       F I  + C  +NP +F   G F F +YYP
Sbjct:   21 KASKYLSEVLSSVNEVELEDVIDSIIDAVEKQPLSSNMIEKSTVETAVQECSRSCDETIEHIFNIIGAFDIPRYVYNSERKKFLPLKMTTLPS--PNLFG-SARDKAELFRERYTILQQRTHRHELFTPPVIGAHPDESRSKFQLKTVETLLGNTAKVGEVIVLGMITQLKEGKFFLEDPTGAVQLDLTNHQFHSGLYTESCFVLAEGWYEDKIFHINAFGFPPTEPSSTTR-AYYGNMNFFGG-PSSTSVKTSVKLRQL-EEENEDAMFVFLSDLWLDQVEVTEKLHTMFSGYSTAPPTCFIFCGNF-SSAPYG---KNHIRSLKDSFKILADIICEYPHIHKSSRFVFVPGPEDPGPGF-------ILPRPPIADHI------TEEFRQRVPFSIFTTNPCRIQYCTQEIVVFREDLVNKMCRNCVRFPN------------NTLD-------------------------IPNHFVKTILSQGLLTPLPPFVSPVYWAYDYALR-LYPLPDLVVIADKYDPFTITNTECLTINPGSFPRSG-FSFKVYYP 511
[Close]
BLAST of DNA polymerase epsilon subunit vs. Ensembl Xenopus
Match: pole2 (polymerase (DNA directed), epsilon 2, accessory subunit [Source:NCBI gene;Acc:595029])

HSP 1 Score: 236.884 bits (603), Expect = 3.620e-70
Identity = 173/558 (31.00%), Postives = 272/558 (48.75%), Query Frame = 3 
Query:  141 FLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKDTNSL--KLFQVIDAFHIPKLVFDCNSKKYIIDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFN-----YHN-------SLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRI---IFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYP 1763
            F  + +A  Y++EV+S+ +  +   V+D +I       L +N I    ++  +  C +  +     +F +I AF IP+ V++   KK++  ++++       LF  S+  K+++ R RY I+ QR  R + F       H         LK +  +L   + +  VI+LGM++Q  EG++FLED TG V+++L N ++H GL  E  FVLAEG YE+K+ +++  G P  EP++ +R A   N+N+FGG P    VK+   +  L + E  D   VFLSD++LDQ  V +KL  +F GY    P  FI CG+F S +  G    + ++ LK    +++     F      S  +FVPGP D   G         L    + D I       +  + R+   IFT+NPCR+ +  +EIV+FRE ++ K+ R+C+  PN            ++LD                         +  + + TILSQ  L PLP  +SPVYW +D AL        LV+       F I  + C  +NP +F   G F F +YYP
Sbjct:   17 FTAQLEASKYLSEVLSSVNEVELEDVIDSIIDAVEKQPLSSNMIEKSTVETAVQECSRSCDETIEHIFNIIGAFDIPRYVYNSERKKFLPLKMTTLPS--PNLFG-SARDKAELFRERYTILQQRTHRHELFTPPVIGAHPDESRSKFQLKTVETLLGNTAKVGEVIVLGMITQLKEGKFFLEDPTGAVQLDLTNHQFHSGLYTESCFVLAEGWYEDKIFHINAFGFPPTEPSSTTR-AYYGNMNFFGG-PSSTSVKTSVKLRQL-EEENEDAMFVFLSDLWLDQVEVTEKLHTMFSGYSTAPPTCFIFCGNF-SSAPYG---KNHIRSLKANSFHLILIHICF--CVNSSRFVFVPGPEDPGPGF-------ILPRPPIADHI------TEEFRQRVPFSIFTTNPCRIQYCTQEIVVFREDLVNKMCRNCVRFPN------------NTLD-------------------------IPNHFVKTILSQGLLTPLPPFVSPVYWAYDYALR-LYPLPDLVVIADKYDPFTITNTECLTINPGSFPRSG-FSFKVYYP 510
[Close]
BLAST of DNA polymerase epsilon subunit vs. Ensembl Xenopus
Match: pole2 (polymerase (DNA directed), epsilon 2, accessory subunit [Source:NCBI gene;Acc:595029])

HSP 1 Score: 236.498 bits (602), Expect = 4.545e-70
Identity = 172/553 (31.10%), Postives = 270/553 (48.82%), Query Frame = 3 
Query:  156 KAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKDTNSL--KLFQVIDAFHIPKLVFDCNSKKYIIDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFN-----YHN-------SLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRI---IFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYP 1763
            +A  Y++EV+S+ +  +   V+D +I       L +N I    ++  +  C +  +     +F +I AF IP+ V++   KK++  ++++       LF  S+  K+++ R RY I+ QR  R + F       H         LK +  +L   + +  VI+LGM++Q  EG++FLED TG V+++L N ++H GL  E  FVLAEG YE+K+ +++  G P  EP++ +R A   N+N+FGG P    VK+   +  L + E  D   VFLSD++LDQ  V +KL  +F GY    P  FI CG+F S +  G    + ++ LK    +++     F      S  +FVPGP D   G         L    + D I       +  + R+   IFT+NPCR+ +  +EIV+FRE ++ K+ R+C+  PN            ++LD                         +  + + TILSQ  L PLP  +SPVYW +D AL        LV+       F I  + C  +NP +F   G F F +YYP
Sbjct:   16 EASKYLSEVLSSVNEVELEDVIDSIIDAVEKQPLSSNMIEKSTVETAVQECSRSCDETIEHIFNIIGAFDIPRYVYNSERKKFLPLKMTTLPS--PNLFG-SARDKAELFRERYTILQQRTHRHELFTPPVIGAHPDESRSKFQLKTVETLLGNTAKVGEVIVLGMITQLKEGKFFLEDPTGAVQLDLTNHQFHSGLYTESCFVLAEGWYEDKIFHINAFGFPPTEPSSTTR-AYYGNMNFFGG-PSSTSVKTSVKLRQL-EEENEDAMFVFLSDLWLDQVEVTEKLHTMFSGYSTAPPTCFIFCGNF-SSAPYG---KNHIRSLKANSFHLILIHICF--CVNSSRFVFVPGPEDPGPGF-------ILPRPPIADHI------TEEFRQRVPFSIFTTNPCRIQYCTQEIVVFREDLVNKMCRNCVRFPN------------NTLD-------------------------IPNHFVKTILSQGLLTPLPPFVSPVYWAYDYALR-LYPLPDLVVIADKYDPFTITNTECLTINPGSFPRSG-FSFKVYYP 504
[Close]
BLAST of DNA polymerase epsilon subunit vs. Ensembl Mouse
Match: Pole2 (polymerase (DNA directed), epsilon 2 (p59 subunit) [Source:MGI Symbol;Acc:MGI:1197514])

HSP 1 Score: 251.906 bits (642), Expect = 7.545e-76
Identity = 168/571 (29.42%), Postives = 283/571 (49.56%), Query Frame = 3 
Query:  126 FKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRK--DTNSLKLFQVIDAFHIPKLVFDCNSKKYI-IDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFN------------YHNSLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRI---IFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYPFTGQVQN 1784
            FKL G LL  +A  Y+TE + +    +  + + K+I       L +N I   +++  +  C +  D     +F +I AF IP+ +++   KK++ +   + A P    LF  + + K+++   RY I++QR  R + F                 LK I  +L + S + +VI+LGM++Q  EG++FLED TG V+++L  A++H GL  E  FVLAEG +E+++ + +  G P  EP++ +R A   NIN+FGG P    VK+   +  L + E  D   V +SD++LDQ  V++K  ++F GY    P  FILCG+F S +  G    ++++ LK+ ++++      +P +   S  +FVPGP D   G     P               ++S     + R+   +FT+NPCR+ +  +EI+IFRE ++ K+ R+C+  P+             SLD                         +  + + TILSQ HL PLP+++ PV+W +D  L        L++       F +  + C  +NP +F   G F F ++YP +  V++
Sbjct:   14 FKLRGLLLRSEATKYLTEALQSVTELELEENLGKIIDAVEKQPLSSNMIERSVVEAAVQECSQSMDETIEHVFNIIGAFDIPRFIYNSERKKFLPLSMTNHAAP---TLFGTARD-KAELYLERYTILHQRTHRHELFTPPVIGSHLEESGSKFQLKTIETLLGSTSKVGDVIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLYTEACFVLAEGWFEDQVFHANAFGFPPTEPSSTTR-AYYGNINFFGG-PSNASVKTYTKLRQL-EDENKDAMFVIVSDVWLDQVQVLEKFHVMFSGYSPAPPTCFILCGNF-SSAPYG---KNQVQALKDSLKSLADIICEYPSIHQSSRFVFVPGPEDPGFGSILPRPPL-------------AESITQEFRQRVPFSVFTTNPCRIQYCTQEIIIFREDLVNKMCRNCVRFPS------------SSLD-------------------------IPTHFVKTILSQGHLAPLPLYVCPVHWAYDYTLR-VYPVPDLLVIADKYDPFTVTNTDCLCINPGSFPRSG-FAFKVFYPSSKTVED 521
[Close]
BLAST of DNA polymerase epsilon subunit vs. Ensembl Mouse
Match: Pole2 (polymerase (DNA directed), epsilon 2 (p59 subunit) [Source:MGI Symbol;Acc:MGI:1197514])

HSP 1 Score: 246.128 bits (627), Expect = 6.357e-74
Identity = 156/517 (30.17%), Postives = 260/517 (50.29%), Query Frame = 3 
Query:  126 FKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRK--DTNSLKLFQVIDAFHIPKLVFDCNSKKYI-IDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFN------------YHNSLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRI---IFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNAL 1622
            FKL G LL  +A  Y+TE + +    +  + + K+I       L +N I   +++  +  C +  D     +F +I AF IP+ +++   KK++ +   + A P    LF  + + K+++   RY I++QR  R + F                 LK I  +L + S + +VI+LGM++Q  EG++FLED TG V+++L  A++H GL  E  FVLAEG +E+++ + +  G P  EP++ +R A   NIN+FGG P    VK+   +  L + E  D   V +SD++LDQ  V++K  ++F GY    P  FILCG+F S +  G    ++++ LK+ ++++      +P +   S  +FVPGP D   G     P               ++S     + R+   +FT+NPCR+ +  +EI+IFRE ++ K+ R+C+  P+             SLD                         +  + + TILSQ HL PLP+++ PV+W +D  L
Sbjct:   14 FKLRGLLLRSEATKYLTEALQSVTELELEENLGKIIDAVEKQPLSSNMIERSVVEAAVQECSQSMDETIEHVFNIIGAFDIPRFIYNSERKKFLPLSMTNHAAP---TLFGTARD-KAELYLERYTILHQRTHRHELFTPPVIGSHLEESGSKFQLKTIETLLGSTSKVGDVIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLYTEACFVLAEGWFEDQVFHANAFGFPPTEPSSTTR-AYYGNINFFGG-PSNASVKTYTKLRQL-EDENKDAMFVIVSDVWLDQVQVLEKFHVMFSGYSPAPPTCFILCGNF-SSAPYG---KNQVQALKDSLKSLADIICEYPSIHQSSRFVFVPGPEDPGFGSILPRPPL-------------AESITQEFRQRVPFSVFTTNPCRIQYCTQEIIIFREDLVNKMCRNCVRFPS------------SSLD-------------------------IPTHFVKTILSQGHLAPLPLYVCPVHWAYDYTL 469
[Close]
BLAST of DNA polymerase epsilon subunit vs. Ensembl Mouse
Match: Gm49384 (predicted gene, 49384 [Source:MGI Symbol;Acc:MGI:6121607])

HSP 1 Score: 47.3654 bits (111), Expect = 2.755e-6
Identity = 27/80 (33.75%), Postives = 43/80 (53.75%), Query Frame = 3 
Query: 1545 TILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYPFTGQVQN 1784
            TILSQ HL PLP+++ PV+W +D  L        L++       F +  + C  +NP +F   G F F ++YP +  V++
Sbjct:    4 TILSQGHLAPLPLYVCPVHWAYDYTLR-VYPVPDLLVIADKYDPFTVTNTDCLCINPGSFPRSG-FAFKVFYPSSKTVED 81
[Close]
BLAST of DNA polymerase epsilon subunit vs. UniProt/SwissProt
Match: sp|Q5ZKQ6|DPOE2_CHICK (DNA polymerase epsilon subunit 2 OS=Gallus gallus OX=9031 GN=POLE2 PE=2 SV=1)

HSP 1 Score: 268.855 bits (686), Expect = 2.002e-81
Identity = 173/574 (30.14%), Postives = 282/574 (49.13%), Query Frame = 3 
Query:  105 KENLKNYFKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRK--DTNSLKLFQVIDAFHIPKLVFDCNSKKYIIDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFNYHN------------SLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRIIFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYPFTGQVQN 1784
            +  L + F++ G LL  +AV Y+TE + + +  +   V+DK+I       L  N I +  ++  +  C +  D     +F +I AF IP+ +++ + K+++   +S        LF  + + K+++ R RY I+ QR  R + F                 LK +  +L   + +R VI+LGM++Q  EG++FLED TG V+++L  A++H GL  E  FVLAEG YE+++ +V+  G P  EP+A +R A   NIN+FGG P    VK+   +  L + E  D   VFLSD++LDQ  V++KL  +F GY    P  F  CG+F S +  G    ++++ LK  ++ +      +P +   S  +FVPGP D   G     P  +           N       L    +FT+NPCR+ +  +EI+IFRE ++ K+ R+C+  P+             S+D                         +  + + TILSQ HL PLP+++SPVYW +D AL        +++       F +  + C  +NP +F   G F F ++YP    V++
Sbjct:    7 RRRLGSAFRVRGLLLRAEAVKYLTETLESLNEEEFEDVIDKIIDAVEKQPLSTNMIELSTVEAAVQECSQALDETIKNVFNIIGAFDIPRYIYNPDRKRFV--PLSMTNLPVPNLFGTARD-KAELFRERYSILQQRTHRHELFTPAAVVIHPDDNRSKFQLKTVETLLGNTAKVREVIVLGMITQLKEGKYFLEDPTGVVQLDLSKAQFHSGLYTESCFVLAEGWYEDEVFHVNAFGFPPTEPSATTR-AFYGNINFFGG-PSSASVKASAKLKQL-EDENEDAMFVFLSDVWLDQAEVLEKLHTMFSGYSSAPPTCFFFCGNF-SSAPYG---KNQIQSLKGSLKALADIICEYPSIHKSSRFVFVPGPEDPGPGSVLPRPPLAE----------NITQEFRQLVPFSVFTTNPCRVQYCTQEIIIFREDLVNKMCRNCVRFPS------------SSMD-------------------------IPNHFVKTILSQGHLTPLPLYVSPVYWAYDYALR-VYPVPDMLVIADKYDPFTVTNTDCLCINPGSFPRSG-FSFKVFYPSNKTVED 521
[Close]
BLAST of DNA polymerase epsilon subunit vs. UniProt/SwissProt
Match: sp|P56282|DPOE2_HUMAN (DNA polymerase epsilon subunit 2 OS=Homo sapiens OX=9606 GN=POLE2 PE=1 SV=2)

HSP 1 Score: 264.233 bits (674), Expect = 1.289e-79
Identity = 179/570 (31.40%), Postives = 294/570 (51.58%), Query Frame = 3 
Query:  126 FKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKDTNSL--KLFQVIDAFHIPKLVFDCNSKKYIIDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFN-----YHN-------SLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRI---IFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYPFTGQVQN 1784
            FKL G LL  +A+ Y+TE + +    +    ++K+I+      L +N I   +++  +  C +  +     +F +I AF IP+ V++   KK++   +++       LF    + K+++ R RY I++QR  R + F       H         LK I  +L + + I + I+LGM++Q  EG++FLED TG V+++L  A++H GL  E  FVLAEG +E+++ +V+  G P  EP++ +R A   NIN+FGG P    VK+   +  L + E  D   VFLSD++LDQ  V++KL+I+F GY    P  FILCG+F S +  G    ++++ LK+ ++ +      +PD+   S  +FVPGP D   G     P               ++S  +  + R+   +FT+NPCR+ +  +EI +FRE ++ K+ R+C+  P+                    N  I N                  + + TILSQ HL PLP+++ PVYW +D AL ++P+PDLLV++D          + C  +NP +F   G F F ++YP    V++
Sbjct:   14 FKLRGLLLRGEAIKYLTEALQSISELELEDKLEKIINAVEKQPLSSNMIERSVVEAAVQECSQSVDETIEHVFNIIGAFDIPRFVYNSERKKFLPLLMTNHPA--PNLFGTPRD-KAEMFRERYTILHQRTHRHELFTPPVIGSHPDESGSKFQLKTIETLLGSTTKIGDAIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLYTEACFVLAEGWFEDQVFHVNAFGFPPTEPSSTTR-AYYGNINFFGG-PSNTSVKTSAKLKQL-EEENKDAMFVFLSDVWLDQVEVLEKLRIMFAGYSPAPPTCFILCGNF-SSAPYG---KNQVQALKDSLKTLADIICEYPDIHQSSRFVFVPGPEDPGFGSILPRPPL-------------AESITNEFRQRVPFSVFTTNPCRIQYCTQEITVFREDLVNKMCRNCVRFPSS-------------------NLAIPN------------------HFVKTILSQGHLTPLPLYVCPVYWAYDYALRVYPVPDLLVIADKYDPFTTTN-TECLCINPGSFPRSG-FSFKVFYPSNKTVED 521
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BLAST of DNA polymerase epsilon subunit vs. UniProt/SwissProt
Match: sp|A7YWS7|DPOE2_BOVIN (DNA polymerase epsilon subunit 2 OS=Bos taurus OX=9913 GN=POLE2 PE=2 SV=1)

HSP 1 Score: 260.766 bits (665), Expect = 2.434e-78
Identity = 170/563 (30.20%), Postives = 286/563 (50.80%), Query Frame = 3 
Query:  126 FKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKDTNSL--KLFQVIDAFHIPKLVFDCNSKKYIIDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFN-----YHN-------SLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRI---IFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYP 1763
            FKL G +L  +AV Y+TE + + +  +    ++K+I       L +N I   +++  +  C +  +     +F +I AF IP+ V++   KK++   +++       LF  + + K+++ R RY I++QR  R + F       H         LK I  +L + S I +VI+LGM++Q  EG++FLED TG ++++L  A++H GL  E  FVLAEG +E+++ +V+  G P  EP++ +R A   N N+FGG P    VK+   +  L + E  D   VF+SD++LDQ  V++KL I+F GY    P  FILCG+F S +  G    ++++ LK+ ++ +      +P++   S  +FVPGP D   G     P               ++S  +  + R+   +FT+NPCR+ +  +EI++FRE ++ K+ R+C+  P                          +S LD           +  + + T+LSQ HL PLP+++ PVYW +D AL        L++       F +  + C  +NP +F   G F F ++YP
Sbjct:   14 FKLRGLMLRGEAVKYLTEALQSINEIELEDALEKIIDAVEKQPLSSNMIERSVVEAAVQECSQSVDETIDHIFNIIGAFDIPRFVYNSERKKFLPLLMTNHPA--PNLFGTARD-KAELFRERYTILHQRTHRHELFTPPVIGSHPDESGSKFQLKTIETLLGSTSKIGDVIVLGMITQLKEGKFFLEDPTGTIQLDLSKAQFHSGLYTESCFVLAEGWFEDQVFHVNAFGFPPTEPSSTTR-AYYGNTNFFGG-PSNTSVKTSAKLKQL-EDENKDAMFVFISDVWLDQVEVLEKLHIMFSGYSPAPPTCFILCGNF-SSAPYG---KNQVQALKDSLKTLADIICGYPNIHQSSRFVFVPGPEDPGFGSILPRPPF-------------AESITNEFRQRVPFSVFTTNPCRIQYCTQEIIVFREDLVNKMCRNCVRFP--------------------------SSNLD-----------IPNHFVKTVLSQGHLTPLPLYVCPVYWAYDYALR-VYPVPDLLVIADKYDPFTLTNTECLCINPGSFPRSG-FSFKVFYP 514
[Close]
BLAST of DNA polymerase epsilon subunit vs. UniProt/SwissProt
Match: sp|O54956|DPOE2_MOUSE (DNA polymerase epsilon subunit 2 OS=Mus musculus OX=10090 GN=Pole2 PE=2 SV=2)

HSP 1 Score: 251.906 bits (642), Expect = 5.278e-75
Identity = 168/571 (29.42%), Postives = 283/571 (49.56%), Query Frame = 3 
Query:  126 FKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRK--DTNSLKLFQVIDAFHIPKLVFDCNSKKYI-IDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFN------------YHNSLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRI---IFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYPFTGQVQN 1784
            FKL G LL  +A  Y+TE + +    +  + + K+I       L +N I   +++  +  C +  D     +F +I AF IP+ +++   KK++ +   + A P    LF  + + K+++   RY I++QR  R + F                 LK I  +L + S + +VI+LGM++Q  EG++FLED TG V+++L  A++H GL  E  FVLAEG +E+++ + +  G P  EP++ +R A   NIN+FGG P    VK+   +  L + E  D   V +SD++LDQ  V++K  ++F GY    P  FILCG+F S +  G    ++++ LK+ ++++      +P +   S  +FVPGP D   G     P               ++S     + R+   +FT+NPCR+ +  +EI+IFRE ++ K+ R+C+  P+             SLD                         +  + + TILSQ HL PLP+++ PV+W +D  L        L++       F +  + C  +NP +F   G F F ++YP +  V++
Sbjct:   14 FKLRGLLLRSEATKYLTEALQSVTELELEENLGKIIDAVEKQPLSSNMIERSVVEAAVQECSQSMDETIEHVFNIIGAFDIPRFIYNSERKKFLPLSMTNHAAP---TLFGTARD-KAELYLERYTILHQRTHRHELFTPPVIGSHLEESGSKFQLKTIETLLGSTSKVGDVIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLYTEACFVLAEGWFEDQVFHANAFGFPPTEPSSTTR-AYYGNINFFGG-PSNASVKTYTKLRQL-EDENKDAMFVIVSDVWLDQVQVLEKFHVMFSGYSPAPPTCFILCGNF-SSAPYG---KNQVQALKDSLKSLADIICEYPSIHQSSRFVFVPGPEDPGFGSILPRPPL-------------AESITQEFRQRVPFSVFTTNPCRIQYCTQEIIIFREDLVNKMCRNCVRFPS------------SSLD-------------------------IPTHFVKTILSQGHLAPLPLYVCPVHWAYDYTLR-VYPVPDLLVIADKYDPFTVTNTDCLCINPGSFPRSG-FAFKVFYPSSKTVED 521
[Close]
BLAST of DNA polymerase epsilon subunit vs. UniProt/SwissProt
Match: sp|Q9VRQ7|DPOE2_DROME (DNA polymerase epsilon subunit 2 OS=Drosophila melanogaster OX=7227 GN=DNApol-epsilon58 PE=1 SV=1)

HSP 1 Score: 188.734 bits (478), Expect = 1.653e-51
Identity = 162/587 (27.60%), Postives = 271/587 (46.17%), Query Frame = 3 
Query:  105 KENLKNYFKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKDT--NSLKLFQVIDAFHIPKLVFDCNSKKYIIDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFN--------------YHNSLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGG-----LPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDIS---DKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRIIFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYPFTGQVQNCNV 1793
            ++ + N FKL GFL+  +  +Y+ E +  FD+A+  K +  +        L    +    L++ IN   +        +F +IDAF +P+  ++   KK+ +D     +P  +QL + +  +KS  ++ RY ++ Q+ +R   F                   L+   N+L T SA++  ++LG+L+Q  EG++++ED TG V+++L  A +H G   EG FVLAEG Y   +L VD +G P  EPA +SR A     N +GG     L    G++  +  N        +  +VFLSD+ LD  +V++KL+ LF GY    P+A +L G F + +    ++    D L  L  G   + K           ++LI VP   D     +  +   +     L  G+  +         R    +NPCR+ +  ++IV+ R  +M K  R+ ++ P +  Q+E+                                     +   TI+ Q HL+P+     PV+W +D AL L+PLPDL+V+ DS  S  + Q  GC VLN  +F  +  F F +Y P T  +++  +
Sbjct:    9 RKRITNTFKLCGFLIRSENSSYLAEQLLPFDAAERDKWLTVITENLQSQKLLTPHVERAALEKAINELNRVGLDEGETVFALIDAFTVPRFRYNQRIKKFELD----TQP--RQLLT-APRMKSDYMQQRYAMLLQKTLRHDLFAPAVIQDGVGAEAQAKKFKLQFAENLLAT-SAMKEAVVLGLLTQLKEGKFYVEDPTGCVQLDLTGARFHAGFFCEGCFVLAEGNYNNGVLKVDGLGFPPAEPANSSR-AFFGTANTWGGESAKLLKYSAGLQELERTNT-------ETTIVFLSDVRLDLPVVMEKLRQLFVGYDSCPPQAIVLMGPFTASTRNHHELRHHLDALGGLAAGCEQLKKQ----------TDLILVPSSED---PTAPNILPRAPIPECLAAGLLKAWP-------RTQLATNPCRLQYCTQQIVVCRLDLMAKFCRNTLHFPEDTSQIEQ-------------------------------------HFARTIVCQGHLVPIHPIAMPVHWDYDPALWLYPLPDLIVMGDSCQSFSSSQ-HGCTVLNTGSF-VKSKFAFKVYIPATRTIEDSEI 520
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BLAST of DNA polymerase epsilon subunit vs. TrEMBL
Match: A0A2C9JIZ0 (DNA polymerase epsilon subunit OS=Biomphalaria glabrata OX=6526 GN=106056840 PE=3 SV=1)

HSP 1 Score: 292.352 bits (747), Expect = 1.550e-87
Identity = 194/580 (33.45%), Postives = 305/580 (52.59%), Query Frame = 3 
Query:  105 KENLKNYFKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKDTNSLK--LFQVIDAFHIPKLVFDCNSKKYIIDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFNYHN-----------SLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRI---IFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYPFTGQVQNCNVN 1796
            K+++ + FK+HG  L  +A +Y+ +V+   DS++  + +DK+I       L +  +  E+ +  +  C  +    K  +F VIDAF IP+  +    KK+I +      P   +L  ++S+ K ++ R RY +++QR +R   F   +            LKP+  ++ + + + NVI+LGML Q  EG+WFLED TG V+++L NAE+  GL  E SFVLAEG YE+ + YVD  G+P  E A  +R +   NIN+FGG P    VKS   +   +++E  D   V LSD++LD   V+ KL ILF+GY  F P AFI CG++LS       +  + K+L +  R++    + + ++   S LIFVPGPND         P            I NS +  ++ + ++   IF+SNPCR+ +  +EI+IFRE I+ KL R+C+  P + +                                      +  +   +I+SQAHL PLP+H+SPVYW +D++L L+PLPD++V +D         ++   V+NP +F    DF F +YYP + QVQ+  V 
Sbjct:    6 KKSIISAFKMHGLTLRSEASSYLQDVLEPVDSSEREQWLDKIIDIVQKQSLSSAMVGKEVCEAAVQECNAEQEDDKESIFNVIDAFSIPRFKYSKERKKFIPESTLLKTP---KLHGEASD-KGEMFRDRYTVLHQRTLRHSLFTPPSLGSVNTDSNKFQLKPVEYLVGSTTKVGNVIVLGMLVQLKEGKWFLEDPTGCVQLDLSNAEFQPGLFTENSFVLAEGHYEDGVFYVDAFGLPPPEKADTTR-SYFGNINFFGG-PSAIQVKSSNRLAQ-IESENTDAMFVLLSDVWLDNGRVMDKLNILFKGYSEFPPTAFIFCGNYLS----SPKVLSQAKILTDCFRDLGNMLSQYQNIIDSSKLIFVPGPNDPGHSTILPRPT-----------IPNSIT--EAFRKKVPSAIFSSNPCRIQYCTQEIIIFREDIVTKLCRNCVRFPKDGN--------------------------------------IPMHFAKSIISQAHLCPLPLHVSPVYWAYDSSLRLYPLPDVIVTADKFDPFSTTAVA-TTVINPGSF-LRTDFGFKVYYPASKQVQDSQVT 521
[Close]
BLAST of DNA polymerase epsilon subunit vs. TrEMBL
Match: A0A210PQ05 (DNA polymerase epsilon subunit OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT09913 PE=3 SV=1)

HSP 1 Score: 282.722 bits (722), Expect = 2.422e-84
Identity = 191/584 (32.71%), Postives = 295/584 (50.51%), Query Frame = 3 
Query:  105 KENLKNYFKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKDTN--SLKLFQVIDAFHIPKLVFDCNSKKYIIDE-ISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSF-------NYHNS----LKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLP---VKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRI---IFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYPFTGQVQNCNVN 1796
            + ++ + FK+HGF L  ++  Y+ EV+ + +  +  + +DK+I T     L ++ I+    +  +  C  + N     +  VIDAF +P+L +  + KK+I ++ +  A P      SD    K+ + R RY I+YQR  R   F       N   S    LKPI  +L + + +  +I+LGML+Q  EG+W+LED +G V +NL  A +H GL  E  FVLAEG YE+ + +++  G P  EPA  +R +   N+N+FGGL    VKG V+  KHI    + E  D   VFLSD++LDQ  V++ L++LF GY  F P  F++CG+FLS        +   K+LKE   ++    + FP++   S  IFVPGP D         P                 S I+ +K +I   +FTSNPCR+ +  ++IV+FRE I+ K+ R+C+  P + D                                      +  +   T+++Q HL PLP+H+ PVYW  D  L L+PLPDL+V +D         +  C V+NP +F    DF F +Y P + QV++  + 
Sbjct:    6 RSHVLSAFKMHGFTLRSESSKYLVEVLGSVEEDQREECLDKIIETIQKQPLSSSLITKATCESAVQECNAEHNDEGENVLCVIDAFSVPRLSYVADRKKFIHNKTLQLASPSLHGTASD----KASLFRDRYTILYQRTQRHDLFTTPAIGSNEEKSKKFKLKPIEFLLGSNTKLGEIIVLGMLTQLKEGKWYLEDPSGAVALNLSKANFHSGLFTENCFVLAEGWYEDSVFHINAFGFPPPEPAKTTR-SYFGNVNFFGGLSNTSVKGSVR-LKHI----EEENPDAMFVFLSDVWLDQVKVLEMLRVLFTGYSEFPPVCFVMCGNFLSAP----QGAQHAKVLKEAFSSLTDLISEFPNILENSRFIFVPGPQDPGPAAILPRPAI-------------PSSIIEDVKRKIPSAVFTSNPCRIQYCTQQIVVFREDIVTKMCRNCVKFPEDGD--------------------------------------VPTHFAKTLIAQGHLCPLPLHVCPVYWSHDAGLRLYPLPDLVVCADKFDPFHKTSVD-CTVMNPGSFP-RTDFSFKVYLPASRQVEDSKIG 522
[Close]
BLAST of DNA polymerase epsilon subunit vs. TrEMBL
Match: V4A559 (DNA polymerase epsilon subunit OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_218232 PE=3 SV=1)

HSP 1 Score: 282.337 bits (721), Expect = 3.222e-84
Identity = 182/579 (31.43%), Postives = 297/579 (51.30%), Query Frame = 3 
Query:  114 LKNYFKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKDTN--SLKLFQVIDAFHIPKLVFDCNSKKYIIDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSF------------NYHNSLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRI---IFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYPFTGQVQNCNVNV 1799
            + + FK+HG  L  ++  Y+ +V+   D       +D +I       L ++ I   + +  I+ C ++ N     +F +IDAF++P+  +  + KK+I +     K     +    +E K+ + R RY +++QR +R   F            N    LKPI  +L + S + ++I+LGML+Q  EG+W++ED TG+V+I++ NA +HKGL  E SFVLAEG YE+ + +++  G P  E A +SR +   N+N+FGG P    VK+   +   ++ +  D   V LSD++LD   V++KLK LF GY  F P  FI CG+F S        +  +K LKE   N+ +  + +P L  V   +FVPGP D           P  ++      I N  +  + ++ +I   IFT+NPCR+ +  +EIVIFRE I+ K+ R+C+  P++ D                                      +  +   +I+SQ HL PLP+H+SP+YW +D +L ++PLPD+LV +D        Q+  C V+NP +FS   ++ F +YYP + +VQ+  +  
Sbjct:    9 ITSAFKMHGLTLRSESSKYLVDVLLPIDEGDREGWLDHVIEIIQKQPLSSSMIDKSVCEAAIHECNQEQNEEGENVFCIIDAFNVPRFTYHPDRKKFIPNSSLGIK---APMLHGDAEDKATLFRERYTVLHQRTLRHDLFTPPVLGAAPDEKNTKFQLKPIEYLLGSTSKLGDIIVLGMLTQMKEGKWYIEDPTGFVQIDISNANFHKGLFTENSFVLAEGWYEDDLFHINAFGFPPTETAKSSR-SYFGNMNFFGG-PTTTSVKASSKLKQ-IEEDNQDAMFVILSDVWLDNVKVMEKLKELFAGYSEFPPTCFIFCGNFTSAP----HGTGHIKTLKESFHNLAEMLSEYPHLLEVCKFVFVPGPQD-----------PGPATILPRPPIPNCIT--EEIRRKIPSAIFTTNPCRLQYCTQEIVIFREDIVTKMCRNCVKFPSDGD--------------------------------------VPTHFAKSIISQGHLCPLPLHVSPIYWAYDRSLRIYPLPDVLVCADKCDPFTTTQVD-CTVMNPGSFS-RTNYGFKVYYPASREVQDSQIQT 524
[Close]
BLAST of DNA polymerase epsilon subunit vs. TrEMBL
Match: A0A4Z2D706 (DNA polymerase epsilon subunit 2 OS=Schistosoma japonicum OX=6182 GN=EWB00_004215 PE=4 SV=1)

HSP 1 Score: 278.87 bits (712), Expect = 1.100e-81
Identity = 196/619 (31.66%), Postives = 304/619 (49.11%), Query Frame = 3 
Query:  105 KENLKNYFKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKD-TNSLKLFQVIDAFHIPKLVFDCNSKKYI--IDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMR----------DKSFNYHNSLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLP-VKGGVKSCKHINNLVQTELG-DKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFA-MFPDLALVSN---------LIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRIIFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPN--------------EKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSY----FLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALE-------XXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNF-----STEGDFP----FNIYYPFTGQVQN 1784
            K  +   FKL+G  L P A  Y+  ++   + +K  KV+ K++ T +   + NN+IS E+ +  +  CR + +  + +  VIDAF +P   F    KK+I  +   SS     + +F  S+  K+ +   RY++IYQR++R          D S N  + L+ I  +L +GS I + ++LG++SQ HEG+W+ ED +G VE+NL  A +H+GL+ EGS  L EG Y++K+LYV  IG+P  E    SR A      + GGLP         K    L  T+ G D  LV L +  LD+   + +L  LF GY    P AFIL G+FLS  ++G    ++  +L++ +R ++  F   FP+ +   N         LI +PGP+D       +LP P L    + D I +   +  S    +   SNPCR+  Y +EIVIFR +  + L RHC+++P               E   L + S++ D   D   ++T+   +   +++ KKN      +L + L   + SQAHLLPL  HI+PVYW  D AL        +  +    +   + S    +    C  +NP  F     S E  F     F +YYP T  V++
Sbjct:    5 KSEVDKLFKLNGLFLAPDASQYILSIVQRVEVSKRQKVLRKLVETILKQKVDNNKISKELCEAAVKECRAEHSTDITVLYVIDAFAVPYFRFSRPLKKFIPFVPADSSEN---RLIFPVSACAKAFLYAHRYEVIYQRMLRHQVFSQTTSLDGSHNTSHRLRQIEYLLASGSRIDSAVVLGLISQVHEGKWYAEDPSGVVELNLQQAIFHEGLLPEGSIALIEGFYDDKILYVTGIGLPPCESPEVSRRAFSVANPFGGGLPDAPAAPLDTKMHKLLTTTQAGSDAMLVILGETHLDKLDTLDQLNTLFAGYSACAPVAFILSGNFLSADSVGKTCPERRIILRKLLRQLLTIFKNNFPESSESQNASQLNQSPQLILIPGPDDPVYAPQHILPRPGLGIDLISDDIKDRSPS--SCPPWLRLASNPCRLRLYTREIVIFRANYSRLLVRHCIHLPTLNALDSCAETDEKIEDSTLRKDSEMTDMDSDGTTSSTVSTPQTKQQISSKKNGKTTVEYLGQCLARCLASQAHLLPLLGHIAPVYWYHDQALSLNPLPNLVVAIEPDSLADLNASEVGLVTTGNCRFINPGRFGQVKYSNESGFKKLYNFKVYYPRTEIVED 618
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BLAST of DNA polymerase epsilon subunit vs. TrEMBL
Match: A0A1S3HPG0 (DNA polymerase epsilon subunit OS=Lingula unguis OX=7574 GN=LOC106157009 PE=3 SV=1)

HSP 1 Score: 275.018 bits (702), Expect = 2.484e-81
Identity = 179/583 (30.70%), Postives = 295/583 (50.60%), Query Frame = 3 
Query:  105 KENLKNYFKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINAC--RKDTNSLKLFQVIDAFHIPKLVFDCNSKKYIIDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFN---------YHNS---LKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRI---IFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYPFTGQVQNCNVNVD 1802
            K  + + FK+HG  L   A  Y+ EV++    A+    +DK++       L +  +S ++ +  +  C   ++ +S ++F VIDAF +P+  +    KK++++      P    L SD+ E K  + R RY +++QR  R   F           H+    L+P+  +L + + + ++I+LGML+Q  EG+WFLED TG V+++L  A +H GL  E  FVLAEG Y++ + +V+  G P  EP+  +R A   NIN+FGG P    +K    +  + Q E  D   V +SD++LD   V+++L+++F GY    P  FI CG+F S +      +  +K L+E  R++      FP L   S  +FVPGP D           P  ++      + N  +  +  + R+   IF SNPCR+ +  +EIV+FRE I+ K+ R+C+  P + D                                      +  +   TI+SQ HL PLP+H+ PVYW +D+AL ++PLPD+++ +D        Q+  C V+NP +F    DF F +Y+P + QV+   +++D
Sbjct:    6 KSQISSAFKMHGLTLRSDATKYLIEVLTPISEAERENGLDKILEILQKQLLSSTMVSRDVCEAAVQECNQEQEEDSDRVFTVIDAFSVPQYSYSYERKKFLLNSNLGLPP--PCLHSDA-EAKCSLYRDRYTLLHQRTSRHDLFTPPALGAQQEQHSKKFKLRPVEFLLGSTAKLGDIIVLGMLTQLKEGKWFLEDTTGAVQLDLTKANFHTGLFTENCFVLAEGWYDDSIFHVNAFGFPPPEPSKTTR-AYFGNINFFGG-PSATSLKMSPKLQQIEQ-ENQDAMFVIVSDVWLDSVKVLERLRLMFVGYAEMPPTCFIFCGNFTSSTG-----TKNIKTLQESFRSLADILKEFPSLVENSRFLFVPGPQD-----------PGPANILPRPALPNVVT--EEFRRRVPSAIFASNPCRLQYCTQEIVVFREDIVTKMCRNCVKFPADGD--------------------------------------IPTHFAKTIISQGHLCPLPLHVCPVYWSYDSALRVYPLPDVVITADKYDPFMVSQVD-CTVINPGSFP-RSDFSFKVYFPASRQVEESRIDID 524
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BLAST of DNA polymerase epsilon subunit vs. Ensembl Cavefish
Match: pole2 (polymerase (DNA directed), epsilon 2 [Source:ZFIN;Acc:ZDB-GENE-020419-16])

HSP 1 Score: 241.891 bits (616), Expect = 5.762e-72
Identity = 164/578 (28.37%), Postives = 282/578 (48.79%), Query Frame = 3 
Query:  105 KENLKNYFKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKDTNSL--KLFQVIDAFHIPKLVFDCNSKKYI-IDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFNY------------HNSLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRI---IFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYPFTGQVQN 1784
            K  +   FK+ G +L P+A  Y+ EV+ + +  +   V+++++       L ++ I + + +  +  C +  +     +F +I AF IP+ ++    KK++ I+  +   P       D    K+++ R RY I+ QR  R + F                 LK +  +L + + +  VI+LGM++Q  EG++FLED TG V++NL  +++H GL  E  FVLAEG YE+ + +V+  G P +EP++ +R A   N+N+FGG P    VK+   +  L + E  D   V  SD++LD   V++K++ +F GY    P  FI CG+F S +  G    +++K +KE ++ +       P++   S  +FVPGP D         P               +++  +  + R+   +FT+NPCR+ +  +EIV+ RE ++ K+ R+C+ +P+                    N  I N                  + + TILSQ HL PLP+++SPV+W +D AL        +++       F+I  S C  +NP +F   G F F +YYP    V++
Sbjct:    7 KTKVSAGFKMRGLMLRPEASRYLVEVLESVNELELDDVIERILDAVEKQPLSSSMIELSVAETAVQDCSQSCDETIDNVFNIIGAFDIPRYIYSTERKKFVPINMTNHPTPSLCGQARD----KAELFRERYTILQQRTHRHELFTAPVIGSAPDEGRNKFQLKTVEALLGSTAKLGEVIVLGMVTQLKEGKFFLEDLTGSVQLNL--SKFHSGLYTESCFVLAEGWYEDSVFHVNAFGFPPIEPSSTTR-AYYGNVNFFGG-PSTTSVKASAKLKQL-EEENEDAMFVVASDVWLDSVEVMEKIQTMFSGYSAAPPTCFIFCGNF-SSAPYG---KNQVKSMKESLKALADLICEHPNIHNSSRFVFVPGPEDPGPSTILPRPPL-------------AENITEEFRQRVPFSVFTTNPCRIQYCSQEIVVIREDLVNKMCRNCVRLPSG-------------------NLDIPN------------------HFVKTILSQGHLTPLPLYVSPVFWAYDYALR-VYPVPDVIIFADKYDPFSISSSDCLSINPGSFPKSG-FSFKVYYPSNRTVED 519
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BLAST of DNA polymerase epsilon subunit vs. Ensembl Sea Lamprey
Match: pole2 (polymerase (DNA directed), epsilon 2 [Source:ZFIN;Acc:ZDB-GENE-020419-16])

HSP 1 Score: 227.639 bits (579), Expect = 1.786e-67
Identity = 160/567 (28.22%), Postives = 265/567 (46.74%), Query Frame = 3 
Query:  126 FKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKDT--NSLKLFQVIDAFHIPKLVFDCNSKKYIIDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFNY----------HNSLKPITNVLTTGSAIR--NVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRIIFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYPFTGQVQN 1784
            FK+HG +L  KA  +++  ++   S +   ++++++       L ++ + + +++  +  CR  +   S  +  +I AF IP+ +++   KK I   +++  P        S   K+ V + RY I+ QR  R   F             N  +  T     GS  R   VI+LGML+Q  EG+++LED TG V+++L   ++H GL  E  FVLAEG YE+++ +V+ +G P  E A  SR    D +N FGG P    VK+   + N ++    D   VF SD++LD+  V+ +L+++F GY    P  FI CG+F S +  G    +   +L + ++ +      FP +   S  +FVPGP D   G         L  S L D I    +         IF +NPCR+ +  +E+VIFRE ++ K+ R+C+  PN                                         +  + + T++SQ HL  LP+ +SPV+W FD  L        L++       F+I  + C  +NP +F    +F F +YYP +  V++
Sbjct:   15 FKMHGLMLRRKARKFLSNALAGLGSVELENMLEQVLDAVQRQPLASSMVELAVVEAAVQECRSSSPEESENVLNIIGAFDIPRFIYNHERKKLIPLAMTNHPP---PCLIASPRDKAAVFQERYAILQQRTHRHDLFTPPVVGSVTEEGRNKFQLKTVEFLQGSTARLGEVIVLGMLTQLKEGKYYLEDPTGTVQLDLKPHQFHSGLYTESCFVLAEGYYEDEVFHVNAVGFPPTEAAKISRTYFGD-VNLFGG-PSTTSVKASAALAN-IEERNTDAMFVFASDVWLDRSDVLDRLRLMFTGYSGAPPTCFIFCGNF-SSAPYG---RNHTSVLIDSLKCLADIICEFPSILESSRFVFVPGPEDPGPG-------AILPRSPLADHI---TAEFRRRVPSAIFATNPCRLQYCTQEVVIFREDLVNKMCRNCVRFPNPGTD-------------------------------------IPTHFVKTLVSQGHLSSLPLFVSPVFWGFDYTLS-LYPLPDLLVCADKYDPFSITNTECMCINPGSFP-RSEFSFKVYYPSSRTVED 522
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BLAST of DNA polymerase epsilon subunit vs. Ensembl Yeast
Match: DPB2 (Second largest subunit of DNA polymerase II (DNA polymerase epsilon); required for maintenance of fidelity of chromosomal replication; essential motif in C-terminus is required for formation of the four-subunit Pol epsilon; expression peaks at the G1/S phase boundary; Cdc28p substrate [Source:SGD;Acc:S000006379])

HSP 1 Score: 111.309 bits (277), Expect = 4.705e-26
Identity = 124/463 (26.78%), Postives = 208/463 (44.92%), Query Frame = 3 
Query:  459 EIKSKVLRSRYQIIYQRLMRDKSFNYHN---------------------------SLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKG-LIIEGSFVLAEGKYEE--KMLYVDTIGMPIVEPAAASRLAICDNINYFG--GLPVKGGV----KSCKHINNLVQTELGDKALVFL-SDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKL---LKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRIIFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIP-NEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNF 1724
            E K ++  +RY +   R+MR+++F   +                           S+ PI N+L  G   +N ++LG+L++  +G W LED +G VEI++      +G   + G  VL EG Y       +V ++ +P  E    + L    N++  G  G+     +    K  K   +L++ EL D   V L +++FLD   ++  L  + Q      P   I  G F SV       S  +      K     +    + F +L   + +IF+PGPNDL+ G    L          +   F  +  I+ +   ++++SNP R+ +  +EIVIFR+ +  +  RH +  P NE + +   +    S D  I    ID    +P+   +K      + L+ TIL Q HL P    + P+ W  D+ L L P+P  +VL D+ S+ F++  +GC V+NP +F
Sbjct:  212 EDKVQMFLTRYYLTNDRVMRNENFQNSDMFNPLSSMVSLQNELSNTNRQQQSSSMSITPIKNLL--GRDAQNFLLLGLLNKNFKGNWSLEDPSGSVEIDISQTIPTQGHYYVPGCMVLVEGIYYSVGNKFHVTSMTLPPGERREIT-LETIGNLDLLGIHGISNNNFIARLDKDLKIRLHLLEKELTDHKFVILGANLFLDDLKIMTALSKILQKLNDDPPTLLIWQGSFTSVPVFASMSSRNISSSTQFKNNFDALATLLSRFDNLTENTTMIFIPGPNDLW-GSMVSLGASGTLPQDPIPSAFTKK--INKVCKNVVWSSNPTRIAYLSQEIVIFRDDLSGRFKRHRLEFPFNESEDVYTENDNMMSKDTDI--VPIDELVKEPDQLPQKVQE--TRKLVKTILDQGHLSPFLDSLRPISWDLDHTLTLCPIPSTMVLCDTTSAQFDLTYNGCKVINPGSF 664
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BLAST of DNA polymerase epsilon subunit vs. Ensembl Nematostella
Match: EDO40154 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7Y2])

HSP 1 Score: 264.233 bits (674), Expect = 3.589e-81
Identity = 181/581 (31.15%), Postives = 292/581 (50.26%), Query Frame = 3 
Query:  105 KENLKNYFKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKDT--NSLKLFQVIDAFHIPKLVFDCNSKKYIIDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFNYHNSL--------------KPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQG-IXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRIIFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYPFTGQVQNCNVN 1796
            ++ + + F+L+G  L+  A  Y+ +V+      +    ++K++ T     L +  +  E+++  +  C +D   +S K F +IDAF +P   F+   KK+      SA          ++E+KS++ + RY +I QR +R + F+    L              K +  +L +  ++ NVI+LGML+Q  EG+++LED TG VE+++   ++H GL  E  FVLAEG Y++++ +V  IG P  EP+  +R A   NIN+FGG P     K+   +  + + E  D   V +SD+ LDQ  V++KL+ L  GY    P  FILCG+F S    G    D+ K+LKE ++      A FP L   S  +F+PGP D   G I    P P      +   I N+            F SNPCR+ +  +E+VIFRE +  K+ R+ +++P         S +KD                            +   L+ T+L+QAHL PLP+H+ PVYW +D +L ++P+PDLL+L+D         L  C   NP +F    DF F +Y+P + +V++C ++
Sbjct:    6 RKKVLSTFRLNGLSLQSDATKYLRDVLVPLSEPEIEDWLEKIVETVQKQPLSSTLVDREVVETAVQECSRDPSEDSDKAFCIIDAFDVPTFAFNSERKKFFPVSGRSA-------LHPNAEVKSELYKERYTVIQQRTIRHELFSNVGDLEAVKQNQTSDKFDLKTVEYLLGSTGSLGNVIVLGMLTQIKEGKFYLEDPTGAVELDITEVKFHTGLYTENCFVLAEGNYDDEIFHVTAIGFPPAEPSKVTR-AQFGNINFFGG-PSTSCAKASSKLKEM-ERENKDAMFVIMSDVHLDQPRVMEKLRTLLTGYSTMPPSLFILCGNFNS-QPYG---PDQYKILKESLQVFASMVAEFPSLVEQSRFLFIPGPQDPGPGNILPRPPIPESLRQVVTTKIPNA-----------TFASNPCRIQYCTQEVVIFREDLTNKMCRNSLHLP---------SDMKD----------------------------IPNQLLKTLLAQAHLCPLPLHVRPVYWGYDASLRVYPIPDLLILADKYDPYTCSALD-CQSTNPGSFP-RSDFMFKVYWPSSKEVEDCKIS 522
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BLAST of DNA polymerase epsilon subunit vs. Ensembl Medaka
Match: pole2 (DNA polymerase epsilon 2, accessory subunit [Source:NCBI gene;Acc:101173901])

HSP 1 Score: 256.914 bits (655), Expect = 6.815e-78
Identity = 170/578 (29.41%), Postives = 286/578 (49.48%), Query Frame = 3 
Query:  105 KENLKNYFKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKDTNSL--KLFQVIDAFHIPKLVFDCNSKKYI-IDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFNYHN------------SLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRI---IFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYPFTGQVQN 1784
            K  +   FK+ G +L P+A  Y+ EV+ + + ++   V++K++ +     L +N I + +++  +  C +  +     +F +I AF +P+ ++    KK++ I+      P        S++ K+++ R RY I+ QR  R + F                 LK I  +L + S +  VI+LGM++Q  EG+++LED +G V+++L  A++H GL  E  FVLAEG YE+ +L+V+  G P  EP++A+R +   NIN+FGG P    VK+   +  L + E  D   V +SD++LD   V++KL I+F GY    P  FILCG+F S +  G     + + LKE ++ +      +P +   S  +FVPGP D         P                Q+  +   +R+   +FT+NPCR+ +  +EI++ RE ++ K+ R+C+ +PN                          S LD           +  + + TILSQ HL PLP+++SPV+W +D +L        +V+       F I  + C  +NP +F   G F F +YYP    V++
Sbjct:    8 KAKVTAGFKMRGLILRPEASRYLAEVLESVNVSELEDVIEKVLDSVEKQPLSSNMIELAVVESAVQDCTQSCDETIDNIFNIIGAFDVPRYIYSVERKKFLPINMTGHPAP----SLCGSAKDKAELFRERYTILQQRTHRHELFTPPAIGAAVEEGQNKFQLKTIEALLGSTSKLGEVIVLGMITQLKEGKFYLEDPSGTVQLDLSKAQFHNGLYTESCFVLAEGWYEDSVLHVNGFGFPPTEPSSATR-SYYGNINFFGG-PSSTSVKASSKLKQL-EEENEDAMFVIVSDVWLDSVEVMEKLNIMFSGYAAMPPTCFILCGNF-SSAPYG---KTQNRSLKESLKALADMICTYPSIHSSSRFVFVPGPEDPGPANILPRPPLV-------------QNITEEFSERVPFSVFTTNPCRIQYCSQEILVIREDLVNKMCRNCIRLPN--------------------------SNLD-----------IPNHFVKTILSQGHLTPLPLYVSPVFWAYDYSLR-VYPVPDVVVFADKYDPFTITNTDCLSVNPGSFPKSG-FTFKVYYPSNKTVED 522
[Close]
BLAST of DNA polymerase epsilon subunit vs. Ensembl Medaka
Match: pole2 (DNA polymerase epsilon 2, accessory subunit [Source:NCBI gene;Acc:101173901])

HSP 1 Score: 239.58 bits (610), Expect = 2.514e-71
Identity = 170/578 (29.41%), Postives = 281/578 (48.62%), Query Frame = 3 
Query:  105 KENLKNYFKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKDTNSL--KLFQVIDAFHIPKLVFDCNSKKYI-IDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFNYHN------------SLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRI---IFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYPFTGQVQN 1784
            K  +   FK+ G +L P+A  Y+ EV+ + + ++   V++K++ +     L +N I + +++  +  C +  +     +F +I AF +P+ ++    KK++ I+      P        S++ K+++ R RY I+ QR  R + F                 LK I  +L + S +  VI+LGM++Q  EG+++LED +G V+++L  +++H GL  E  FVLAEG YE+ +L+V+  G P  EP++A+R +   NIN+FGG P    VK+   +  L + E  D   V +SD++LD   V++KL I+F GY    P  FILCG+F S +  G   +  LK    G  N   S          S  +FVPGP D         P                Q+  +   +R+   +FT+NPCR+ +  +EI++ RE ++ K+ R+C+ +P                          NS LD           +  + + TILSQ HL PLP+++SPV+W +D +L        +V+       F I  + C  +NP +F   G F F +YYP    V++
Sbjct:    8 KAKVTAGFKMRGLILRPEASRYLAEVLESVNVSELEDVIEKVLDSVEKQPLSSNMIELAVVESAVQDCTQSCDETIDNIFNIIGAFDVPRYIYSVERKKFLPINMTGHPAP----SLCGSAKDKAELFRERYTILQQRTHRHELFTPPAIGAAVEEGQNKFQLKTIEALLGSTSKLGEVIVLGMITQLKEGKFYLEDPSGTVQLDL--SKFHNGLYTESCFVLAEGWYEDSVLHVNGFGFPPTEPSSATR-SYYGNINFFGG-PSSTSVKASSKLKQL-EEENEDAMFVIVSDVWLDSVEVMEKLNIMFSGYAAMPPTCFILCGNF-SSAPYGKTQNRSLK----GDLNADSS------KPCSSRFVFVPGPEDPGPANILPRPPLV-------------QNITEEFSERVPFSVFTTNPCRIQYCSQEILVIREDLVNKMCRNCIRLP--------------------------NSNLD-----------IPNHFVKTILSQGHLTPLPLYVSPVFWAYDYSLR-VYPVPDVVVFADKYDPFTITNTDCLSVNPGSFPKSG-FTFKVYYPSNKTVED 513
[Close]
BLAST of DNA polymerase epsilon subunit vs. Planmine SMEST
Match: SMESG000037681.1 (SMESG000037681.1)

HSP 1 Score: 1076.23 bits (2782), Expect = 0.000e+0
Identity = 564/570 (98.95%), Postives = 567/570 (99.47%), Query Frame = 3 
Query:   96 MNPKENLKNYFKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKDTNSLKLFQVIDAFHIPKLVFDCNSKKYIIDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFNYHNSLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPNDLFQGIXXXXXXXXXXXXXXXDGIFNSQSNIDSLKDRIIFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALEXXXXXXXXXXXXXXXXXXNIQLSGCNVLNPFNFSTEGDFPFNIYYPFTGQVQNCNVNVDM 1805
            MNPKENLKNYFKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKDTNSLKLFQVIDA+H PKLVFDCNSKKYIIDEISSAKPFFQQLFSDSSEIK KVLRSRYQIIYQRLMRDKSFNYHNSLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLS STIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGP+DLFQGISSVLPHPSLSSSYLLDGIFNSQSNIDSLKDRIIFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNAL+LFPLPDLLVLSDSDSSSFNIQLSGCNVLNPFNFSTEGDFPFNIYYPFTGQVQNCNVNVDM
Sbjct:    1 MNPKENLKNYFKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFMDLGLPNNRISVEILKQTINACRKDTNSLKLFQVIDAYHTPKLVFDCNSKKYIIDEISSAKPFFQQLFSDSSEIKFKVLRSRYQIIYQRLMRDKSFNYHNSLKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYHKGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGGLPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGYKIFKPEAFILCGDFLSASTIGMDISDKLKLLKEGIRNVVKSFAMFPDLALVSNLIFVPGPSDLFQGISSVLPHPSLSSSYLLDGIFNSQSNIDSLKDRIIFTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSLDDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISPVYWQFDNALQLFPLPDLLVLSDSDSSSFNIQLSGCNVLNPFNFSTEGDFPFNIYYPFTGQVQNCNVNVDM 570
The following BLAST results are available for this feature:
BLAST of DNA polymerase epsilon subunit vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
POLE22.685e-8031.40DNA polymerase epsilon 2, accessory subunit [Sourc... [more]
POLE29.247e-7931.40DNA polymerase epsilon 2, accessory subunit [Sourc... [more]
POLE24.948e-7230.28DNA polymerase epsilon 2, accessory subunit [Sourc... [more]
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BLAST of DNA polymerase epsilon subunit vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pole-21.843e-5229.79Probable DNA polymerase epsilon subunit 2 [Source... [more]
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BLAST of DNA polymerase epsilon subunit vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
DNApol-epsilon581.650e-5227.60gene:FBgn0035644 transcript:FBtr0077081[more]
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BLAST of DNA polymerase epsilon subunit vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pole24.175e-4629.56polymerase (DNA directed), epsilon 2 [Source:ZFIN;... [more]
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BLAST of DNA polymerase epsilon subunit vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pole29.271e-8031.75polymerase (DNA directed), epsilon 2, accessory su... [more]
pole24.096e-7531.40polymerase (DNA directed), epsilon 2, accessory su... [more]
pole24.868e-7431.28polymerase (DNA directed), epsilon 2, accessory su... [more]
pole23.620e-7031.00polymerase (DNA directed), epsilon 2, accessory su... [more]
pole24.545e-7031.10polymerase (DNA directed), epsilon 2, accessory su... [more]
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BLAST of DNA polymerase epsilon subunit vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Pole27.545e-7629.42polymerase (DNA directed), epsilon 2 (p59 subunit)... [more]
Pole26.357e-7430.17polymerase (DNA directed), epsilon 2 (p59 subunit)... [more]
Gm493842.755e-633.75predicted gene, 49384 [Source:MGI Symbol;Acc:MGI:6... [more]
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BLAST of DNA polymerase epsilon subunit vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q5ZKQ6|DPOE2_CHICK2.002e-8130.14DNA polymerase epsilon subunit 2 OS=Gallus gallus ... [more]
sp|P56282|DPOE2_HUMAN1.289e-7931.40DNA polymerase epsilon subunit 2 OS=Homo sapiens O... [more]
sp|A7YWS7|DPOE2_BOVIN2.434e-7830.20DNA polymerase epsilon subunit 2 OS=Bos taurus OX=... [more]
sp|O54956|DPOE2_MOUSE5.278e-7529.42DNA polymerase epsilon subunit 2 OS=Mus musculus O... [more]
sp|Q9VRQ7|DPOE2_DROME1.653e-5127.60DNA polymerase epsilon subunit 2 OS=Drosophila mel... [more]
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BLAST of DNA polymerase epsilon subunit vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2C9JIZ01.550e-8733.45DNA polymerase epsilon subunit OS=Biomphalaria gla... [more]
A0A210PQ052.422e-8432.71DNA polymerase epsilon subunit OS=Mizuhopecten yes... [more]
V4A5593.222e-8431.43DNA polymerase epsilon subunit OS=Lottia gigantea ... [more]
A0A4Z2D7061.100e-8131.66DNA polymerase epsilon subunit 2 OS=Schistosoma ja... [more]
A0A1S3HPG02.484e-8130.70DNA polymerase epsilon subunit OS=Lingula unguis O... [more]
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BLAST of DNA polymerase epsilon subunit vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pole25.762e-7228.37polymerase (DNA directed), epsilon 2 [Source:ZFIN;... [more]
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BLAST of DNA polymerase epsilon subunit vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pole21.786e-6728.22polymerase (DNA directed), epsilon 2 [Source:ZFIN;... [more]
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BLAST of DNA polymerase epsilon subunit vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
DPB24.705e-2626.78Second largest subunit of DNA polymerase II (DNA p... [more]
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BLAST of DNA polymerase epsilon subunit vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO401543.589e-8131.15Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of DNA polymerase epsilon subunit vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
pole26.815e-7829.41DNA polymerase epsilon 2, accessory subunit [Sourc... [more]
pole22.514e-7129.41DNA polymerase epsilon 2, accessory subunit [Sourc... [more]
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BLAST of DNA polymerase epsilon subunit vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000037681.10.000e+098.95SMESG000037681.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031663 ID=SMED30031663|Name=DNA polymerase epsilon subunit|organism=Schmidtea mediterranea sexual|type=transcript|length=1808bp
TTTTGTTTTAAAATGTTTGTTTTAAATTTTTAATTTGATATTTAAATTCA
ATCTTTTTAAAATTACAGAATTTTTGATTTTATTATTTTTGTGACATGAA
TCCCAAAGAAAATTTGAAAAATTATTTTAAGTTACACGGATTCCTTCTGG
AACCAAAAGCGGTTACTTACGTAACTGAGGTTATTTCTAATTTTGACTCA
GCTAAATGTACTAAAGTTGTTGACAAAATGATTTCAACATTTATGGATCT
CGGCTTGCCTAACAATAGAATCTCGGTAGAAATTCTTAAACAAACTATTA
ATGCATGTCGAAAAGATACAAATTCATTGAAACTTTTCCAAGTAATCGAT
GCATTTCATATTCCAAAATTAGTGTTTGATTGCAACTCTAAAAAGTATAT
TATTGATGAAATTTCCAGCGCGAAACCATTTTTCCAACAATTATTCTCAG
ATAGTTCCGAAATAAAATCTAAAGTTTTGCGCAGTAGATATCAAATAATT
TATCAGCGACTAATGCGAGACAAATCATTTAATTACCACAATTCATTAAA
ACCAATTACAAATGTTTTAACCACTGGATCAGCAATAAGAAACGTAATCA
TATTGGGAATGTTATCCCAATTTCACGAAGGCCGTTGGTTTCTTGAGGAT
GAAACGGGATATGTAGAAATTAATTTAGATAATGCTGAATATCACAAAGG
ATTGATAATTGAGGGTTCTTTTGTTCTGGCAGAGGGTAAATATGAAGAAA
AAATGTTATATGTTGATACAATCGGAATGCCGATAGTTGAGCCTGCAGCG
GCAAGTCGTTTAGCCATTTGTGATAACATCAATTATTTTGGAGGGTTGCC
TGTAAAGGGTGGTGTCAAATCGTGCAAGCACATCAATAATTTGGTACAAA
CTGAATTAGGCGACAAAGCTCTCGTTTTTCTATCGGACATTTTTCTCGAC
CAAGAAATCGTTATACAAAAACTAAAAATCCTCTTTCAAGGATATAAAAT
CTTTAAACCGGAAGCATTTATTCTGTGTGGCGATTTCCTCTCGGTCTCAA
CAATCGGTATGGATATATCAGATAAACTAAAACTCCTGAAAGAGGGAATT
AGAAACGTGGTGAAAAGTTTTGCCATGTTTCCCGATTTGGCACTCGTCAG
CAATTTGATATTTGTTCCTGGACCGAATGATTTGTTCCAGGGCATTTCCA
GTGTTTTGCCGCATCCGAGTCTGTCTTCGTCTTATTTATTGGATGGGATT
TTCAATTCACAATCAAACATCGATAGCCTTAAAGATCGAATCATTTTCAC
CTCTAATCCCTGTCGGATGACTTTCTACGATAAGGAAATTGTGATATTTC
GAGAATCGATCATGCAAAAGCTGTATCGACATTGCATGTATATCCCGAAT
GAGAAAGATCAGTTAGAGGAAATCTCTCAATTAAAGGATTCTCTCGATGA
CTCGATAAGAAACACAACAATCGATAACAGTGAACTTGATCCTGAAGTTG
CTGGCAAGAAGAATAGCTATTTCCTTGAAAAGAATCTGATTTGGACGATA
TTATCTCAGGCGCATTTATTGCCTTTGCCAATACATATCAGTCCGGTTTA
TTGGCAGTTTGACAATGCTCTAGAATTGTTTCCCCTTCCAGATTTACTAG
TGCTCTCAGACTCGGATTCGTCATCATTCAATATCCAATTGAGTGGTTGC
AATGTTCTGAATCCATTCAATTTCTCGACTGAAGGCGATTTCCCGTTTAA
TATCTATTATCCTTTCACGGGCCAAGTGCAAAACTGCAATGTCAATGTTG
ATATGTAG
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protein sequence of SMED30031663-orf-1

>SMED30031663-orf-1 ID=SMED30031663-orf-1|Name=SMED30031663-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=571bp
MNPKENLKNYFKLHGFLLEPKAVTYVTEVISNFDSAKCTKVVDKMISTFM
DLGLPNNRISVEILKQTINACRKDTNSLKLFQVIDAFHIPKLVFDCNSKK
YIIDEISSAKPFFQQLFSDSSEIKSKVLRSRYQIIYQRLMRDKSFNYHNS
LKPITNVLTTGSAIRNVIILGMLSQFHEGRWFLEDETGYVEINLDNAEYH
KGLIIEGSFVLAEGKYEEKMLYVDTIGMPIVEPAAASRLAICDNINYFGG
LPVKGGVKSCKHINNLVQTELGDKALVFLSDIFLDQEIVIQKLKILFQGY
KIFKPEAFILCGDFLSVSTIGMDISDKLKLLKEGIRNVVKSFAMFPDLAL
VSNLIFVPGPNDLFQGISSVLPHPSLSSSYLLDGIFNSQSNIDSLKDRII
FTSNPCRMTFYDKEIVIFRESIMQKLYRHCMYIPNEKDQLEEISQLKDSL
DDSIRNTTIDNSELDPEVAGKKNSYFLEKNLIWTILSQAHLLPLPIHISP
VYWQFDNALELFPLPDLLVLSDSDSSSFNIQLSGCNVLNPFNFSTEGDFP
FNIYYPFTGQVQNCNVNVDM*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0008622epsilon DNA polymerase complex
GO:0005634nucleus
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000420parapharyngeal region
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR016266POLE2
IPR007185DNA_pol_a/d/e_bsu
IPR024639DNA_pol_e_bsu_N
Vocabulary: biological process
TermDefinition
GO:0006261DNA-dependent DNA replication
GO:0006260DNA replication
GO:0071897DNA biosynthetic process
Vocabulary: molecular function
TermDefinition
GO:0003887DNA-directed DNA polymerase activity
GO:0003677DNA binding
GO:0016740transferase activity
GO:0016779nucleotidyltransferase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016266DNA polymerase epsilon, subunit BPIRSFPIRSF000799DNA_pol_epsilon_2coord: 2..568
e-value: 2.4E-105
score: 350.4
IPR016266DNA polymerase epsilon, subunit BPANTHERPTHR12708DNA POLYMERASE EPSILON SUBUNIT Bcoord: 5..562
IPR007185DNA polymerase alpha/delta/epsilon, subunit BPFAMPF04042DNA_pol_E_Bcoord: 277..528
e-value: 7.3E-31
score: 107.2
NoneNo IPR availableGENE3DG3DSA:1.10.8.60coord: 2..72
e-value: 4.5E-5
score: 25.1
IPR024639DNA polymerase epsilon subunit B, N-terminalPFAMPF12213Dpoe2NTcoord: 6..71
e-value: 1.6E-9
score: 37.9