Gag-Pol polyprotein

Overview
NameGag-Pol polyprotein
Smed IDSMED30031648
Length (bp)2124
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Gag-Pol polyprotein (SMED30031648) t-SNE clustered cells

Violin plots show distribution of expression levels for Gag-Pol polyprotein (SMED30031648) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Gag-Pol polyprotein (SMED30031648) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Gag-Pol polyprotein (SMED30031648) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30031648

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30031648 dd_Smed_v4_26635_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30031648 dd_Smed_v4_26635_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30031648 dd_Smed_v4_26635_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Gag-Pol polyprotein vs. Ensembl Zebrafish
Match: FO704673.1 (pep chromosome:GRCz11:12:14545475:14551077:-1 gene:ENSDARG00000112601.1 transcript:ENSDART00000188717.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:FO704673.1)

HSP 1 Score: 77.411 bits (189), Expect = 4.177e-14
Identity = 80/317 (25.24%), Postives = 135/317 (42.59%), Query Frame = 1 
Query: 1096 TVSLPRREFLLAKM*ETVKDLLKRCVTCAK----RKID*GKTKEILIPRESSEFLEHIFMHVAVMERXXXXXXXXXXXXDRLSK---LVSLTATSNQDEKTIWKTLMKNWIYKYGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNGLVERAIITMRDLLVTTMKGGCAEKQ--WHELLPRIEFSINATYQSATKYYPFEIVYGRKITLNANSGLQNQVQDISKKTQ-----MNS-----KQAVKKMREFKDD-KRIK*NFEVGEEILVCQEPHR----RNKYIIQYDGPYKILRFISEHLIELQYPT 1974
            T++L R  F   KM + +   +K C  CA+    R++  G  + + IP        H+ +              ++VIIDR SK   L+ L       E  +   L ++    YG P+ I++DRG  F +K  K    QL I     + Y  + NG VER    +   +   ++  C+ +Q  W   LP  E++ N+   S+T   PF+ + G +  +   SG  + V  +    Q      NS     ++A++  R   D  +R   N++ G+ + +     R      K   +Y GP+KIL+ I+     L+ P 
Sbjct: 1000 TLNLVRNAFWWPKMNQDITTFVKSCAVCAQSKTPRELPSGLLQPLPIPHRP---WSHLSIDFVTDLPNSNNYTTILVIIDRFSKACRLIPLKGLPTAMETAL--ELFQHVFRVYGIPEDIVSDRGPQFTSKVWKAFCKQLDINVSLTSGYHPESNGQVER----LNQEIGRYLRTYCSREQDKWSNFLPWAEYAQNSLTHSSTGLTPFQCILGYQPPMFPWSGEPSMVPSVDDWVQRSEEVWNSAHVRLQRAIRTQRINADQRRRPNPNYQPGQRVWLSTRDLRLRLPSRKLSPRYVGPFKILKRINNVTYRLELPA 1307

HSP 2 Score: 66.2402 bits (160), Expect = 1.758e-13
Identity = 51/153 (33.33%), Postives = 79/153 (51.63%), Query Frame = 1 
Query:  175 PQ*AKQLRPFLGLANYYKRFFKDYXXXXXXXXXXXSGCDKTIKLSENCE*SFNQLKDALTQTPIIDFPRIDWKFILDTDASFEAIGAV*SQIKEDGSDI-VIAYS*RHLTAITET----RKKLFALHEYVLCFRQYFYGK--EFVTRTDHKAL 612
            P+  K+L+ FLG AN+Y+RF ++Y+ ++APL +   G    +K +     SF +LK + T  PI+  P  +  F+++ DAS   IGAV SQ   +   +   AY  R LTA         K+L ++   +  +R +  G    F   TDHK L
Sbjct:  727 PKTVKELQRFLGFANFYRRFIRNYSLISAPLTSLLKGKPSKLKWNPETVKSFEKLKTSFTTAPILKHPNPELPFVVEVDASDYGIGAVLSQRHGNPGKLHPCAYFSRKLTAAERNYDVGNKELLSMKAALEEWRHWLEGAVHPFQIITDHKNL 879

HSP 3 Score: 30.0314 bits (66), Expect = 1.758e-13
Identity = 12/26 (46.15%), Postives = 19/26 (73.08%), Query Frame = 2 
Query:   86 SKLKFLGYIISEDKIQSNSEKIKSIT 163
            SK  FLGYIIS   ++ N+ K++++T
Sbjct:  697 SKTSFLGYIISHHGVEMNNTKVQAVT 722
[Close]
BLAST of Gag-Pol polyprotein vs. Ensembl Zebrafish
Match: CABZ01030006.1 (pep scaffold:GRCz11:KN150258.1:33006:36502:-1 gene:ENSDARG00000114113.1 transcript:ENSDART00000182402.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CABZ01030006.1)

HSP 1 Score: 69.3218 bits (168), Expect = 3.027e-12
Identity = 71/287 (24.74%), Postives = 121/287 (42.16%), Query Frame = 1 
Query: 1147 VKDLLKRCVTCAKRKID*----GKTKEILIPRESSEFLEHIFMHVAVMERXXXXXXXXXXXXDRLSKLVSLTATSNQDEKTIWKTLMKNWIYKYGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNGLVERAIITMRDLLVTTMKGGCAEKQWHELLPRIEFSINATYQSATKYYPFEIVYGRKI-----------TLNANSGLQNQVQDI---SKKTQMNSKQAVKKMREFKDDKRIK*NFEVGEEILVCQEP------HRRNKYIIQYDGPYKILR 1935
            +K  +K C  C   K D     GK +++   R +  +   I   +  M ++    +Y++V +D  SK V L    +   +TI   L +  + ++G P  IL+DRG  F +    E  G+  I  +  T+Y  Q N + ER   T++ ++   ++     K W   LP + F++N+  Q +    P E+  GRKI            L+      N V  I    ++ + N  +A K+     D  R    F   E + V   P      H   K   ++ GPY+I++
Sbjct:    5 IKKYVKNCAKCQVTKWDNRKPAGKLQQVTTSRPNEMWRVDI---MGPMPKSGKQNEYLLVFVDYFSKWVELFPMRHATAQTIATILRQEMLTRWGVPDFILSDRGAQFVSSLFTELCGKWNITPKLTTAYHPQTN-MTERVNRTLKSMIAGFVEDN--HKTWDTYLPELRFALNSAIQESIGMTPAELHLGRKIHSPMDKLLHRRDLSPTKPAYNMVHKIIQLQRQAKENYTKAQKRQLRSYDKNRRDVFFRERERVWVRNFPISSAQHHFSAKLAPKWKGPYRIIQ 285
[Close]
BLAST of Gag-Pol polyprotein vs. Ensembl Zebrafish
Match: CR354395.2 (pep chromosome:GRCz11:12:13553730:13556536:-1 gene:ENSDARG00000101547.2 transcript:ENSDART00000166396.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR354395.2)

HSP 1 Score: 65.4698 bits (158), Expect = 1.776e-10
Identity = 51/153 (33.33%), Postives = 78/153 (50.98%), Query Frame = 1 
Query:  175 PQ*AKQLRPFLGLANYYKRFFKDYXXXXXXXXXXXSGCDKTIKLSENCE*SFNQLKDALTQTPIIDFPRIDWKFILDTDASFEAIGAV*SQIKEDGSDI-VIAYS*RHLTAITET----RKKLFALHEYVLCFRQYFYG--KEFVTRTDHKAL 612
            PQ  K+L+ FLG AN+Y+RF + ++ +AAPL A        +  S     +F+ LK   T  PI+  P  D  FI++ DAS   +GAV SQ +   S +   A+  R LT+         ++L A+   +  +R +  G  ++F   TDHK L
Sbjct:  398 PQNLKELQRFLGFANFYRRFIRGFSSIAAPLTAMTKRNSHKLSWSSEARQAFSDLKTQFTTAPILRHPNPDLPFIVEVDASNTGVGAVLSQRQGQPSKMYPCAFFSRKLTSAERNYDVGNRELLAMKLALEEWRHWLEGASQQFTILTDHKNL 550
[Close]
BLAST of Gag-Pol polyprotein vs. Ensembl Zebrafish
Match: CR533578.3 (pep chromosome:GRCz11:16:23466094:23471387:-1 gene:ENSDARG00000115891.1 transcript:ENSDART00000180899.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR533578.3)

HSP 1 Score: 65.4698 bits (158), Expect = 1.912e-10
Identity = 51/153 (33.33%), Postives = 78/153 (50.98%), Query Frame = 1 
Query:  175 PQ*AKQLRPFLGLANYYKRFFKDYXXXXXXXXXXXSGCDKTIKLSENCE*SFNQLKDALTQTPIIDFPRIDWKFILDTDASFEAIGAV*SQIKEDGSDIV-IAYS*RHLTAITET----RKKLFALHEYVLCFRQYFYG--KEFVTRTDHKAL 612
            PQ  K+L+ FLG AN+Y+RF + ++ +AAPL A        +  S     +F+ LK   T  PI+  P  D  FI++ DAS   +GAV SQ +   S +   A+  R LT+         ++L A+   +  +R +  G  ++F   TDHK L
Sbjct:  740 PQNLKELQRFLGFANFYRRFIRGFSSIAAPLTAMTKRNSHKLSWSSEARQAFSDLKTQFTTAPILRHPNPDLPFIVEVDASNTGVGAVLSQRQGQPSKMYPCAFFSRKLTSAERNYDVGNRELLAMKLALEEWRHWLEGASQQFTILTDHKNL 892
[Close]
BLAST of Gag-Pol polyprotein vs. Ensembl Zebrafish
Match: BX571665.1 (pep chromosome:GRCz11:17:35431724:35437222:1 gene:ENSDARG00000111789.1 transcript:ENSDART00000190293.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX571665.1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.927e-10
Identity = 51/153 (33.33%), Postives = 78/153 (50.98%), Query Frame = 1 
Query:  175 PQ*AKQLRPFLGLANYYKRFFKDYXXXXXXXXXXXSGCDKTIKLSENCE*SFNQLKDALTQTPIIDFPRIDWKFILDTDASFEAIGAV*SQIKEDGSDIV-IAYS*RHLTAITET----RKKLFALHEYVLCFRQYFYG--KEFVTRTDHKAL 612
            PQ  K+L+ FLG AN+Y+RF + ++ +AAPL A        +  S     +F+ LK   T  PI+  P  D  FI++ DAS   +GAV SQ +   S +   A+  R LT+         ++L A+   +  +R +  G  ++F   TDHK L
Sbjct:  733 PQNLKELQRFLGFANFYRRFIRGFSSIAAPLTAMTKRNSHKLSWSSEARQAFSDLKTQFTTAPILRHPNPDLPFIVEVDASNTGVGAVLSQRQGQPSKMYPCAFFSRKLTSAERNYDVGNRELLAMKLALEEWRHWLEGASQQFTILTDHKNL 885
[Close]
BLAST of Gag-Pol polyprotein vs. Ensembl Xenopus
Match: anxa6 (annexin A6 [Source:Xenbase;Acc:XB-GENE-989741])

HSP 1 Score: 80.4925 bits (197), Expect = 5.038e-20
Identity = 54/151 (35.76%), Postives = 78/151 (51.66%), Query Frame = 1 
Query:  175 PQ*AKQLRPFLGLANYYKRFFKDYXXXXXXXXXXXS-GCDKTIKLSENCE*SFNQLKDALTQTPIIDFPRIDWKFILDTDASFEAIGAV*SQIKEDGSDIVIAYS*RHL----TAITETRKKLFALHEYVLCFRQYFYGKEFVTRTDHKAL 612
            P+  KQ+  FLG + YY++F  +Y+ +A PL    S    +TI  +  CE + N LK AL  +P++  P    +FIL TDAS   +GAV SQ+   G +  +AY  R L     A     K+  A+   +   + Y YG+EF   TDH  L
Sbjct:  560 PKTQKQVLAFLGTSGYYRKFIPNYSTVAKPLTDLTSRQRSRTIVWTPECESAMNALKQALASSPVLAAPDFSRRFILQTDASNFGLGAVLSQVNTYGEEHPVAYLSRKLLPREAAYATIEKECLAIVWALQKLQPYLYGREFTVVTDHNPL 710

HSP 2 Score: 38.5058 bits (88), Expect = 5.038e-20
Identity = 15/28 (53.57%), Postives = 21/28 (75.00%), Query Frame = 3 
Query:  654 WVASLSEYNFQLKYRKSEEHANADGLSK 737
            W   L +YNF +++RK +EH NADGLS+
Sbjct:  726 WSLLLQQYNFTIQHRKGKEHHNADGLSR 753

HSP 3 Score: 70.4774 bits (171), Expect = 6.187e-12
Identity = 53/194 (27.32%), Postives = 92/194 (47.42%), Query Frame = 1 
Query: 1411 KYGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNGLVERAIITMRDLLVTTMKGGCAEKQWHELLPRIEFSINATYQSATKYYPFEIVYGRKITLNAN-------SGLQNQVQDI--------SKKTQMNS------KQAVKKMREFKDDKRIK*NFEVGEEILVCQEPHRRNKYIIQYDGPYKI 1929
            + G P  IL+D+G  F ++ L+    + G+R   ++ Y  Q NGL ER   T++ +L T ++ G  EK W   LP + F+     Q +T + PFE++YGR++    +          Q+Q   I         +  QM S        A ++ + + D K  +  F  G+++L+   P R +K    ++GPY +
Sbjct:   31 RVGFPSEILSDQGPQFTSQLLQCLWQRCGVRAIHSSPYHPQTNGLCERFNGTLKTMLRTFVESG--EKDWERYLPHLLFAYREVPQESTGFSPFELLYGRRVRGPLDLLCEYWEGAPQSQEVPIIPYVLKFRQRLEQMTSLAHDHLSAAQQRQKVWYDRKARERRFMEGDKVLLL-VPTRHDKLQAAWEGPYVV 221
[Close]
BLAST of Gag-Pol polyprotein vs. Ensembl Xenopus
Match: ENSXETT00000024810.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:KV464121.1:1437:3201:1 gene:ENSXETG00000014958.1 transcript:ENSXETT00000024810.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 70.8626 bits (172), Expect = 7.974e-17
Identity = 53/158 (33.54%), Postives = 74/158 (46.84%), Query Frame = 1 
Query:  154 VNYKRTKPQ*AKQLRPFLGLANYYKRFFKDYXXXXXXXXXXXSGC-DKTIKLSENCE*SFNQLKDALTQTPIIDFPRIDWKFILDTDASFEAIGAV*SQIKEDGSDIVIAYS*RHL----TAITETRKKLFALHEYVLCFRQYFYGKEFVTRTDHKAL 612
            VN+    P   KQ+  FLG A YY+RF  +Y+ +A PL    S    + +  +  C  + + LK AL   P++  P     FI+ TDAS   IGAV SQ+ E G +  I Y  R L     A     K+  A+   +   + Y +G  F   TDH  L
Sbjct:  105 VNWP--TPTTQKQVLAFLGTAGYYRRFIPNYSAIAKPLTDLTSKRRPRVVTWTPECATAMSALKSALVNAPVLYAPDFSRGFIVHTDASTYGIGAVLSQVDEKGGEHPIIYLSRKLLPREVAYATIEKECLAIVWALKKLQPYLFGSAFTVVTDHNPL 260

HSP 2 Score: 37.3502 bits (85), Expect = 7.974e-17
Identity = 16/43 (37.21%), Postives = 25/43 (58.14%), Query Frame = 3 
Query:  654 WVASLSEYNFQLKYRKSEEHANADGLSKI-GVVCAHNVRQHIY 779
            W  +L ++NF +++RK   H NADGLS+  G  C    R  ++
Sbjct:  276 WSLALQQFNFTIQHRKGSHHGNADGLSRRDGEDCTGQGRPTVF 318
[Close]
BLAST of Gag-Pol polyprotein vs. Ensembl Xenopus
Match: ENSXETT00000008059.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:3:106110826:106112135:-1 gene:ENSXETG00000002142.1 transcript:ENSXETT00000008059.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 79.7221 bits (195), Expect = 3.174e-15
Identity = 75/312 (24.04%), Postives = 128/312 (41.03%), Query Frame = 1 
Query: 1093 ETVSLPRREFLLAKM*ETVKDLLKRCVTCAKRKID*GKTKEILIPRE-SSEFLEHIFMHVAVMERXXXXXXXXXXXXDRLSKLVSLTATSNQDEKTIWKTLMKNWIYK-YGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNGLVERAIITMRDLLVTTMKGGCAEKQWHELLPRIEFSINATYQSATKYYPFEIVYGRKIT----------LNANSGLQNQVQDISKKTQMNSKQAVKKMREFKDDKR-IK*NFEVGEEILVCQEPHR----RNKYIIQYDGPYKILRFISEHLIELQYPTQ 1977
            +T+ L RR      + + V+D +  C  CA  K    +   +L P    S    H+ M   V          + V+IDR SK+                 L    I++ +G P  I++DRG  F +++ +     LG+  +F+++Y  Q NG  ER    +   L   +     +  W +LLP  EF+ N    S+T   PF  VYG+             + A   L   +  I   T+ N +++    + F D +R     ++VGE++ +  +  R      K   ++ GP+ I   I+   + LQ P +
Sbjct:   29 KTLELLRRLVWWPTIRKDVRDFVAACTVCATTKASHSRPCGLLHPLPVPSRPWTHLGMDFIVELPPSCGNTVIWVVIDRFSKMAHFVPLKKLPSAVELAQLFIQHIFRLHGFPVEIVSDRGSQFVSRFWRSLCKSLGVSLQFSSAYHPQTNGAAERVNQALEQFLRNHV--SLCQDDWSDLLPWAEFAHNNASHSSTGRSPFLSVYGQHPLAFPQDFLLSEVPAADDLAAHMSVIWAATKSNLEKSSLVHKTFADRRRKPSPPYKVGEKVWLSSKNIRLKVPSPKLGPKFLGPFSISEVINPVAVRLQLPPE 338
[Close]
BLAST of Gag-Pol polyprotein vs. Ensembl Xenopus
Match: npm1 (nucleophosmin (nucleolar phosphoprotein B23, numatrin) [Source:Xenbase;Acc:XB-GENE-1019571])

HSP 1 Score: 79.7221 bits (195), Expect = 8.725e-15
Identity = 75/312 (24.04%), Postives = 128/312 (41.03%), Query Frame = 1 
Query: 1093 ETVSLPRREFLLAKM*ETVKDLLKRCVTCAKRKID*GKTKEILIPRE-SSEFLEHIFMHVAVMERXXXXXXXXXXXXDRLSKLVSLTATSNQDEKTIWKTLMKNWIYK-YGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNGLVERAIITMRDLLVTTMKGGCAEKQWHELLPRIEFSINATYQSATKYYPFEIVYGRKIT----------LNANSGLQNQVQDISKKTQMNSKQAVKKMREFKDDKR-IK*NFEVGEEILVCQEPHR----RNKYIIQYDGPYKILRFISEHLIELQYPTQ 1977
            +T+ L RR      + + V+D +  C  CA  K    +   +L P    S    H+ M   V          + V+IDR SK+                 L    I++ +G P  I++DRG  F +++ +     LG+  +F+++Y  Q NG  ER    +   L   +     +  W +LLP  EF+ N    S+T   PF  VYG+             + A   L   +  I   T+ N +++    + F D +R     ++VGE++ +  +  R      K   ++ GP+ I   I+   + LQ P +
Sbjct:  286 KTLELLRRLVWWPTIRKDVRDFVAACTVCATTKASHSRPCGLLHPLPIPSRPWTHLGMDFIVELPPSCGNTVIWVVIDRFSKMAHFVPLRKLPSAVELAHLFVQHIFRLHGFPVEIVSDRGSQFVSRFWRSLCKSLGVSLQFSSAYHPQTNGAAERVNQALEQFLRNHV--SLCQDDWSDLLPWAEFAHNNASHSSTGRSPFLSVYGQHPLAFPQDFLLSEVPAADDLAAHMSVIWAATKSNLEKSSLVHKTFADRRRKPSPPYKVGEKVWLSSKNIRLKVPSPKLGPKFLGPFSISEVINPVAVRLQLPPE 595
[Close]
BLAST of Gag-Pol polyprotein vs. Ensembl Xenopus
Match: mknk2 (MAPK interacting serine/threonine kinase 2 [Source:Xenbase;Acc:XB-GENE-491527])

HSP 1 Score: 78.1814 bits (191), Expect = 9.648e-15
Identity = 74/312 (23.72%), Postives = 127/312 (40.71%), Query Frame = 1 
Query: 1093 ETVSLPRREFLLAKM*ETVKDLLKRCVTCAKRKID*GKTKEILIPRE-SSEFLEHIFMHVAVMERXXXXXXXXXXXXDRLSKLVSLTATSNQDEKTIWKTLMKNWIYK-YGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNGLVERAIITMRDLLVTTMKGGCAEKQWHELLPRIEFSINATYQSATKYYPFEIVYGRKIT----------LNANSGLQNQVQDISKKTQMNSKQAVKKMREFKDDKR-IK*NFEVGEEILVCQEPHR----RNKYIIQYDGPYKILRFISEHLIELQYPTQ 1977
            +T+ L RR      + + V+D +  C  CA  K    +   +L P    S    H+ M   V          + V+IDR SK+                 L    I++ +G P  I++DRG  F +++ +     LG+  +F+++Y  Q NG  ER    +   L   +     +  W +LLP  EF+ N    S+T   PF  VYG+             + A   L   +  I   T+ N +++    + F D +R     ++VG+++ +     R      K   ++ GP+ I   I+   + LQ P +
Sbjct:   29 KTLELLRRLVWWPTIRKDVRDFVAACTVCATTKASHSRPCGLLHPLPIPSRPWTHLGMDFIVELPPSCGNTVIWVVIDRFSKMAHFIPLRKLPSAVELAHLFVQHIFRLHGFPVEIVSDRGSQFVSRFWRSLCKSLGVSLQFSSAYHPQTNGAAERVNQALEQFLRNHVS--LCQDDWSDLLPWAEFAHNNASHSSTGRSPFLSVYGQHPLAFPQDFLLSEVPAADDLAAHMSVIWAATKSNLEKSSLVHKTFADRRRKPSPPYKVGDKVWLSSRNIRLRVPSPKLGPKFVGPFSISEVINPVAVRLQLPPE 338
[Close]
BLAST of Gag-Pol polyprotein vs. UniProt/SwissProt
Match: sp|P20825|POL2_DROME (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 4.926e-16
Identity = 53/148 (35.81%), Postives = 78/148 (52.70%), Query Frame = 1 
Query:  175 PQ*AKQLRPFLGLANYYKRFFKDYXXXXXXXXXXXSGCDK--TIKLSENCE*SFNQLKDALTQTPIIDFPRIDWKFILDTDASFEAIGAV*SQIKEDGSDIVIAYS*RHLTAITETRKKLFALHEYVLCFRQYFYGKEFVTRTDHKAL 612
            P   K++R FLGL  YY++F  +YA +A P+ +      K  T KL E  E +F +LK  + + PI+  P  + KF+L TDAS  A+GAV SQ     S I    +  H    +   K+L A+      FR Y  G++F+  +DH+ L
Sbjct:  435 PTKDKEIRAFLGLTGYYRKFIPNYADIAKPMTSCLKKRTKIDTQKL-EYIE-AFEKLKALIIRDPILQLPDFEKKFVLTTDASNLALGAVLSQNGHPISFISRTLN-DHELNYSAIEKELLAIVWATKTFRHYLLGRQFLIASDHQPL 579

HSP 2 Score: 35.039 bits (79), Expect = 4.926e-16
Identity = 15/39 (38.46%), Postives = 24/39 (61.54%), Query Frame = 3 
Query:  642 QFQTWVASLSEYNFQLKYRKSEEHANADGLSKIGVVCAH 758
            + + W   LSEY F++ Y K +E++ AD LS+I +   H
Sbjct:  591 KLERWRVRLSEYQFKIDYIKGKENSVADALSRIKIEENH 629

HSP 3 Score: 28.1054 bits (61), Expect = 4.926e-16
Identity = 12/32 (37.50%), Postives = 21/32 (65.62%), Query Frame = 2 
Query:   68 CDLITKSKLKFLGYIISEDKIQSNSEKIKSIT 163
            C+ + K +  FLG+I++ D I+ N  K+K+I 
Sbjct:  400 CEFL-KKEANFLGHIVTPDGIKPNPIKVKAIV 430
[Close]
BLAST of Gag-Pol polyprotein vs. UniProt/SwissProt
Match: sp|P04323|POL3_DROME (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 2.329e-15
Identity = 48/148 (32.43%), Postives = 73/148 (49.32%), Query Frame = 1 
Query:  169 TKPQ*AKQLRPFLGLANYYKRFFKDYXXXXXXXXXXXSGCDKTIKLSENCE*SFNQLKDALTQTPIIDFPRIDWKFILDTDASFEAIGAV*SQIKEDGSDIVIAYS*RHLTAITETRKKLFALHEYVLCFRQYFYGKEFVTRTDHKAL 612
            TKP   K+++ FLGL  YY++F  ++A +A P+        K    +   + +F +LK  +++ PI+  P    KF L TDAS  A+GAV SQ     S I    +  H    +   K+L A+      FR Y  G+ F   +DH+ L
Sbjct:  437 TKP---KEIKAFLGLTGYYRKFIPNFADIAKPMTKCLKKNMKIDTTNPEYDSAFKKLKYLISEDPILKVPDFTKKFTLTTDASDVALGAVLSQDGHPLSYISRTLN-EHEINYSTIEKELLAIVWATKTFRHYLLGRHFEISSDHQPL 580

HSP 2 Score: 34.2686 bits (77), Expect = 2.329e-15
Identity = 14/31 (45.16%), Postives = 21/31 (67.74%), Query Frame = 3 
Query:  654 WVASLSEYNFQLKYRKSEEHANADGLSKIGV 746
            W   LSE++F +KY K +E+  AD LS+I +
Sbjct:  596 WRVKLSEFDFDIKYIKGKENCVADALSRIKL 626

HSP 3 Score: 30.8018 bits (68), Expect = 2.329e-15
Identity = 13/33 (39.39%), Postives = 23/33 (69.70%), Query Frame = 2 
Query:   68 CDLITKSKLKFLGYIISEDKIQSNSEKIKSITK 166
            C+ + K +  FLG++++ D I+ N EKI++I K
Sbjct:  401 CEFL-KQETTFLGHVLTPDGIKPNPEKIEAIQK 432
[Close]
BLAST of Gag-Pol polyprotein vs. UniProt/SwissProt
Match: sp|P10394|POL4_DROME (Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaster OX=7227 GN=POL PE=4 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 1.402e-14
Identity = 49/152 (32.24%), Postives = 79/152 (51.97%), Query Frame = 1 
Query:  175 PQ*AKQLRPFLGLANYYKRFFKDYXXXXXXXXXXXSGCDKTIKL--SENCE*SFNQLKDALTQTPIIDFPRIDWKFILDTDASFEAIGAV*SQIKEDGSDIVIAYS*RHLTA----ITETRKKLFALHEYVLCFRQYFYGKEFVTRTDHKAL 612
            P  A   R F+   NYY+RF K++A  +  +      C K +    ++ C+ +F  LK  L    ++ +P    +F + TDAS +A GAV +Q   +G  + +AY+ R  T      + T ++L A+H  ++ FR Y YGK F  +TDH+ L
Sbjct:  544 PHDADSARRFVAFCNYYRRFIKNFADYSRHITRL---CKKNVPFEWTDECQKAFIHLKSQLINPTLLQYPDFSKEFCITTDASKQACGAVLTQ-NHNGHQLPVAYASRAFTKGESNKSTTEQELAAIHWAIIHFRPYIYGKHFTVKTDHRPL 691

HSP 2 Score: 72.4034 bits (176), Expect = 1.170e-11
Identity = 73/301 (24.25%), Postives = 142/301 (47.18%), Query Frame = 1 
Query: 1093 ETVSLPRREFLLAKM*ETVKDLLKRCVTCAKRKID*GKTKEILIPRESSEFLEHIFMHVAV-----MERXXXXXXXXXXXXDRLSKLVSLTATSNQDEKTIWKTLMKNWIYKYGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNGLVERAIITMRDLLVTTMKGGCAEKQWHELLPRIEFSINATYQSATKYYPFEIVYGRKITLNANSGLQNQVQ------DISKKTQMNSKQAVKKMREFKD----------DKRIK-*NFEVGEEILVCQEPHRRNKYIIQYDGPYKI 1929
            +T++  +R +    M + +K+ +++C  C K K     TK    P   +E  EH F  V V     + ++    +Y + +I  L+K +     +N+  KT+ K + +++I KYG  +T +TD G  ++N  + +    L I+   +T++ HQ  G+VER+  T+ + + + +     +  W   L    +  N T      Y P+E+V+GR   L  +    + ++      D +K+++   + A  + R+  +          D ++K    EVG+++L+  E    +K   +Y GPYKI
Sbjct:  914 KTLAKVKRHYYWKNMSKYIKEYVRKCQKCQKAK----TTKHTKTPMTITETPEHAFDRVVVDTIGPLPKSENGNEYAVTLICDLTKYLVAIPIANKSAKTVAKAIFESFILKYGPMKTFITDMGTEYKNSIITDLCKYLKIKNITSTAHHHQTVGVVERSHRTLNEYIRSYI--STDKTDWDVWLQYFVYCFNTTQSMVHNYCPYELVFGRTSNLPKHFNKLHSIEPIYNIDDYAKESKYRLEVAYARARKLLEAHKEKNKENYDLKVKDIELEVGDKVLLRNE--VGHKLDFKYTGPYKI 1206
[Close]
BLAST of Gag-Pol polyprotein vs. UniProt/SwissProt
Match: sp|Q8I7P9|POL5_DROME (Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 2.245e-13
Identity = 61/161 (37.89%), Postives = 78/161 (48.45%), Query Frame = 1 
Query:  175 PQ*AKQLRPFLGLANYYKRFFKDYXXXXXXXXXXXSGCDKTIK----------LSENCE*SFNQLKDALTQTPIIDFPRIDWKFILDTDASFEAIGAV*SQIKEDGSDIVIAYS*RHLTAITET----RKKLFALHEYVLCFRQYFYGKEFV-TRTDHKAL 612
            P   K+L+ FLG+ +YY++F +DYAK+A PL     G    IK          L E    SFN LK  L  + I+ FP     F L TDAS  AIGAV SQ  + G D  IAY  R L    E      K++ A+   +   R Y YG   +   TDH+ L
Sbjct:  352 PTSVKELKRFLGMTSYYRKFIQDYAKVAKPLTNLTRGLYANIKSSQSSKVPITLDETALQSFNDLKSILCSSEILAFPCFTKPFHLTTDASNWAIGAVLSQ-DDQGRDRPIAYISRSLNKTEENYATIEKEMLAIIWSLDNLRAYLYGAGTIKVYTDHQPL 511

HSP 2 Score: 30.4166 bits (67), Expect = 2.245e-13
Identity = 12/38 (31.58%), Postives = 23/38 (60.53%), Query Frame = 3 
Query:  627 KSISPQFQTWVASLSEYNFQLKYRKSEEHANADGLSKI 740
            ++ + + + W A + EYN +L Y+  + +  AD LS+I
Sbjct:  518 RNFNAKLKRWKARIEEYNCELIYKPGKSNVVADALSRI 555
[Close]
BLAST of Gag-Pol polyprotein vs. UniProt/SwissProt
Match: sp|Q87040|POL_SFVCP (Pro-Pol polyprotein OS=Simian foamy virus (isolate chimpanzee) OX=298339 GN=pol PE=3 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 9.161e-10
Identity = 56/221 (25.34%), Postives = 102/221 (46.15%), Query Frame = 1 
Query: 1150 KDLLK---RCVTCAKRKID*GKTKEILIPRESSEFLEHIFMHVAVMERXXXXXXXXXXXXDRLSKLVSLTATSNQDEKTIWKTLMKNWIYKYGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNGLVERAIITMRDLLVTTMKGGCAEKQWHELLPRIEFSINATYQSATKYYPFEIVYGRKITLNANSGLQNQ-VQDISKKTQMNSKQAVK 1800
            KD++K   RC  C         +  IL P    +  +  F+             YV+VI+D ++    L  T         K+L  N +     P+ I +D+G  F +    E   + GI  EF+T Y  Q +G VER    ++ LL   + G     +W++LLP ++ ++N TY    KY P ++++G    +++N+   NQ   D++++ +++  Q ++
Sbjct:  838 KDVVKQLGRCKQCLITNASNKTSGPILRPDRPQKPFDKFFIDYIGPLPPSQGYLYVLVIVDGMTGFTWLYPTKAPSTSATVKSL--NVLTSIAIPKVIHSDQGAAFTSSTFAEWAKERGIHLEFSTPYHPQSSGKVERKNSDIKRLLTKLLVG--RPTKWYDLLPVVQLALNNTYSPVLKYTPHQLLFG----IDSNTPFANQDTLDLTREEELSLLQEIR 1050
[Close]
BLAST of Gag-Pol polyprotein vs. TrEMBL
Match: A0A3B3DB17 (Uncharacterized protein OS=Oryzias melastigma OX=30732 PE=4 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 1.084e-40
Identity = 64/163 (39.26%), Postives = 100/163 (61.35%), Query Frame = 1 
Query:  175 PQ*AKQLRPFLGLANYYKRFFKDYXXXXXXXXXXXSGCDKTIKLSENCE*SFNQLKDALTQTPIIDFPRIDWKFILDTDASFEAIGAV*SQIKEDGSDIVIAYS*RHLT----AITETRKKLFALHEYVLCFRQYFYGKEFVTRTDHKAL*TRQKNQ*VHNFR 651
            P+   +++ FLGLA+YY+RF + +A +A PLH  A    K  + ++ C+ +F+QLK +LT  P++ +P    +FILDTDAS   IGAV SQ +E G + V+AY+ R L+        T+K+L ++  +   F+ Y  GKEF+ RTDH +L      + +HNF+
Sbjct:  533 PKNVTEVQSFLGLASYYRRFVRGFADIAKPLHQLAEK-GKRFQWNDACQKAFDQLKISLTTAPVLAYPDPKKQFILDTDASDLGIGAVLSQ-EEGGLEKVVAYASRALSKQERQYATTKKELLSMVTFTRHFKHYLLGKEFILRTDHNSL------RWLHNFQ 687

HSP 2 Score: 84.3445 bits (207), Expect = 1.084e-40
Identity = 71/302 (23.51%), Postives = 142/302 (47.02%), Query Frame = 1 
Query: 1141 ETVKDLLKRCVTCAKRKID*GKTKEILIPRES---SEFLEHIFMHV-AVMERXXXXXXXXXXXXDRLSKLVSLTATSNQDEKTIWKTLMKNWIYKYGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNGLVERAIITMRDLLVTTMKGGCAEKQWHELLPRIEFSINATYQSATKYYPFEIVYGRKITLNAN------------------SGLQNQVQDISKKTQMNSKQAVKKMREFKDDKRIK*NFEV---GEEILVCQEPHRRN---KYIIQYDGPYKILRFISEHLIEL 1962
            + VK  ++ CV C  RK      K+   P ++   S   E + + +   +  T    KY++VI D  SK        NQ+ +++ K L + W+ ++G P++I +D+GRNFE+   KE    L I +   + Y  Q +G++ER   T+  +L   ++    +  W  LLP +  +  ++  ++T + P+++++G+++ L  +                  S LQ  +  +    + +  +A ++ +E  D    + NF+    GE + V  +  +R    K   ++ GP+++L  ++E L  L
Sbjct:  872 QDVKAWVRECVDCGSRK---AHGKQPCAPMQTFAPSRPFERVALDILGPLPETPNRNKYILVIGDYFSKWTEAFPLQNQEAQSVAKVLTEEWVCRFGAPRSIHSDQGRNFESTLFKELCNLLNIHKSRTSPYHPQSDGMIERFNRTLLSMLAMFVEDN--QLNWDTLLPYVMLAYRSSVHASTSFTPYKVLFGQEVVLPVDIMLNVGEHETFSSVDQYVSRLQETLSSVVDAVKRHQTRASEQQKESYD---FRVNFQYYSEGELVWVHNKARKRGVCAKLQKRFKGPFRVLERLTEVLYRL 1165

HSP 3 Score: 33.4982 bits (75), Expect = 1.084e-40
Identity = 12/40 (30.00%), Postives = 22/40 (55.00%), Query Frame = 3 
Query:  642 QFQTWVASLSEYNFQLKYRKSEEHANADGLSKIGVVCAHN 761
            Q   W   L+ + +++ +R  + H+NAD LS++     HN
Sbjct:  692 QLARWTEQLANFEYKIVHRPGKLHSNADALSRLPGCVGHN 731

HSP 4 Score: 27.335 bits (59), Expect = 1.084e-40
Identity = 30/108 (27.78%), Postives = 50/108 (46.30%), Query Frame = 2 
Query:  839 MIEIMEEQKKDKLFKEAMEILRNEIGV*NESFQNSFLFKYKD---QLKKSDDMLV-IEMD*IDL-----VVIPKSYQSKLVIKIH--EDLCHIGIKKLFHYLEGNFFW 1129
            M E++E Q+KD   ++ +           +S +   L KY     QLK     LV I  D  D      VV+PKS   +++ ++H      H+G++KL   ++  F+W
Sbjct:  763 MDEMVEAQRKDTELRQLINCKEEAACSLPDSPE---LQKYAPVWHQLKIQKSRLVRIPPDNSDAAACVQVVLPKSMVPQVLRQLHNVSTGGHLGVQKLQAKVKDRFYW 867
[Close]
BLAST of Gag-Pol polyprotein vs. TrEMBL
Match: A0A4Y2GDB0 (Transposon Ty3-I Gag-Pol polyprotein OS=Araneus ventricosus OX=182803 GN=TY3B-I_628 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 9.271e-28
Identity = 67/151 (44.37%), Postives = 88/151 (58.28%), Query Frame = 1 
Query:  172 KPQ*AKQLRPFLGLANYYKRFFKDYXXXXXXXXXXXSGCDKTIKLSENCE*SFNQLKDALTQTPIIDFPRIDWKFILDTDASFEAIGAV*SQIKEDGSDIVIAYS*RHLTAITE----TRKKLFALHEYVLCFRQYFYGKEFVTRTDHKAL 612
            +P+    LR FLGL  YY+RF K+++ +A PLH            +E CE SFN LK ALT +PI+ +PR D  FILDTDAS E IGAV SQ      + VIAY  + L         TRK+L A+ + +  F  Y YG++F+ RTDH +L
Sbjct:  826 RPETVHDLRSFLGLCTYYRRFVKNFSTIAKPLHKLTEA-KSNFNWTEECEKSFNSLKQALTSSPILTYPRTDKDFILDTDASNEGIGAVLSQ-NIGNEEHVIAYFSKSLGKPERNYCVTRKELLAIVKSIEHFHHYLYGRKFLLRTDHASL 974

HSP 2 Score: 40.0466 bits (92), Expect = 9.271e-28
Identity = 20/59 (33.90%), Postives = 31/59 (52.54%), Query Frame = 3 
Query:  642 QFQTWVASLSEYNFQLKYRKSEEHANADGLSKIGVVCAHNVRQHIYNINKKNQK*DISI 818
            Q   W+  L EY+F++++RK   H NAD LS+    C  + +Q      K   + DIS+
Sbjct:  986 QIARWIQRLQEYDFEIQHRKGTSHGNADALSR--RPCKESCKQCTNAEKKFGMERDISV 1042

HSP 3 Score: 30.0314 bits (66), Expect = 9.271e-28
Identity = 16/53 (30.19%), Postives = 31/53 (58.49%), Query Frame = 2 
Query:    2 FNDLIK*FQQICC*LNAFNFADCDLITKSKLKFLGYIISEDKIQSNSEKIKSI 160
             N+L K FQ++       N   C    K ++ +LG++IS + ++++ EKIK++
Sbjct:  770 LNNLRKVFQRLQKANLKLNLKKCRFFQK-EVTYLGHVISAEGVKTDPEKIKAV 821
[Close]
BLAST of Gag-Pol polyprotein vs. TrEMBL
Match: Q94BM2 (Gag-pol polyprotein OS=Hordeum vulgare OX=4513 PE=4 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.196e-27
Identity = 58/200 (29.00%), Postives = 90/200 (45.00%), Query Frame = 1 
Query: 1120 FLLAKM*ETVKDLLKRCVTCAKRKI---D*GKTKEILIPRESSEFLEHIFMHVAVMERXXXXXXXXXXXXDRLSKLVSLTATSNQDEKT-IWKTLMKNWIYKYGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNGLVERAIITMRDLLVTTMKGGCAEKQWHELLPRIEFSINATYQSATKYYPFEIVYG 1707
            F   +M   V+  + RC TC K K      G    + +P    E +   F  V  + RT   +  + V++DR SK+         D+   +     +  I  +G P TI++DR   F + + +    +LG +  F+T+   Q +G  E    ++  +L   +K     K W E LP IEF+ N +  S TK  PFEIVYG
Sbjct: 1281 FFWPRMRRDVERFVARCTTCQKAKSRLNPHGLYMPLPVPSVPWEDISMDF--VLGLPRTKKGRDSIFVVVDRFSKMAHFIPCHKSDDAANVADLFFREIIRLHGVPNTIVSDRDAKFLSHFWRCLWAKLGTKLLFSTTCHPQTDGQTEVVNRSLSTMLRAVLKNNI--KLWEECLPHIEFAYNRSLHSTTKMCPFEIVYG 1476

HSP 2 Score: 63.929 bits (154), Expect = 1.196e-27
Identity = 56/175 (32.00%), Postives = 89/175 (50.86%), Query Frame = 1 
Query:  172 KPQ*AKQLRPFLGLANYYKRFFKDYXXXXXXXXXXXSGCDKTIKLSENCE*SFNQLKDALTQTPIIDFPRIDWKFILDTDASFEAIGAV*SQIKEDGSDIVIAYS*RHLTA----ITETRKKLFALHEYVLCFRQYFYGKEFVTRTDHKAL*TRQKNQ*VHNFRHG-WLVYRNTI 681
            +P+   Q+R FLG A +Y+RF +D++ +AAPL+      D         E +F  LKD LT  P++  P  +  F L+ DAS   +G V   + +DG    +AY    L+      +   K+L+AL   +  ++ Y + KEFV  +DH++L    K+Q   N RH  W+ +  T 
Sbjct: 1000 QPKTVTQVRSFLGXAGFYRRFVRDFSTIAAPLNELTKK-DVPYSWGTAQEEAFTVLKDKLTHAPLLQLPDFNKTFELECDASGIGLGGV---LLQDGKP--VAYFSEKLSGPSLNYSTYDKELYALVRTLETWQHYLWPKEFVIHSDHESL-KHIKSQAKLNRRHAKWVEFIETF 1167

HSP 3 Score: 27.7202 bits (60), Expect = 1.196e-27
Identity = 11/35 (31.43%), Postives = 20/35 (57.14%), Query Frame = 2 
Query:   56 NFADCDLITKSKLKFLGYIISEDKIQSNSEKIKSI 160
            N   C   T  ++ FLGY+++   I+ +  KI++I
Sbjct:  962 NLGKCTFCT-DRVSFLGYVVTPQGIEVDKAKIEAI 995

HSP 4 Score: 26.5646 bits (57), Expect = 1.196e-27
Identity = 32/133 (24.06%), Postives = 55/133 (41.35%), Query Frame = 2 
Query:  776 IQHKQEKPKV-------RYINLIQ---KTQTMIEIMEEQKKDKLFKEAMEILRNEIGV*NESFQNSFLFKYKDQLKKSDDMLVIEMD*IDLVVIPKSYQSKLVIKIHEDLCHIGIKKLFHYLEGNFFWQKCKK 1144
            I+HK+ K  V       RY  L Q   K   +  I ++   D  FK+ +E  R           N F+F+         + L I    I L+++ +++   L       + H G+KK+   L  +FFW + ++
Sbjct: 1171 IKHKKGKDNVIADALSRRYTMLSQLDFKIFGLETIKDQYVHDADFKDVLENCREGRTWNKFIINNGFVFRA--------NKLCIPASSIRLLLLQEAHGGGL-------MGHFGVKKMEDVLATHFFWPRMRR 1288
[Close]
BLAST of Gag-Pol polyprotein vs. TrEMBL
Match: A0A4Y2GA36 (Transposon Ty3-I Gag-Pol polyprotein OS=Araneus ventricosus OX=182803 GN=TY3B-I_431 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 1.361e-27
Identity = 67/151 (44.37%), Postives = 88/151 (58.28%), Query Frame = 1 
Query:  172 KPQ*AKQLRPFLGLANYYKRFFKDYXXXXXXXXXXXSGCDKTIKLSENCE*SFNQLKDALTQTPIIDFPRIDWKFILDTDASFEAIGAV*SQIKEDGSDIVIAYS*RHLTAITE----TRKKLFALHEYVLCFRQYFYGKEFVTRTDHKAL 612
            +P+    LR FLGL  YY+RF K+++ +A PLH            +E CE SFN LK ALT +PI+ +PR D  FILDTDAS E IGAV SQ      + VIAY  + L         TRK+L A+ + +  F  Y YG++F+ RTDH +L
Sbjct:  819 RPETVHDLRSFLGLCTYYRRFVKNFSTIAKPLHKLTEA-KSNFNWTEECEKSFNSLKQALTSSPILTYPRTDKDFILDTDASNEGIGAVLSQ-NIGNEEHVIAYFSKSLGKPERNYCVTRKELLAIVKSIEHFHHYLYGRKFLLRTDHASL 967

HSP 2 Score: 39.2762 bits (90), Expect = 1.361e-27
Identity = 20/59 (33.90%), Postives = 31/59 (52.54%), Query Frame = 3 
Query:  642 QFQTWVASLSEYNFQLKYRKSEEHANADGLSKIGVVCAHNVRQHIYNINKKNQK*DISI 818
            Q   W+  L EY+F++++RK   H NAD LS+    C  + +Q      K   + DIS+
Sbjct:  979 QIARWIQRLQEYDFEIQHRKGTSHGNADALSR--RPCKESCKQCTNAEKKFGMERDISV 1035

HSP 3 Score: 30.0314 bits (66), Expect = 1.361e-27
Identity = 16/53 (30.19%), Postives = 31/53 (58.49%), Query Frame = 2 
Query:    2 FNDLIK*FQQICC*LNAFNFADCDLITKSKLKFLGYIISEDKIQSNSEKIKSI 160
             N+L K FQ++       N   C    K ++ +LG++IS + ++++ EKIK++
Sbjct:  763 LNNLRKVFQRLQKANLKLNLKKCRFFQK-EVTYLGHVISAEGVKTDPEKIKAV 814
[Close]
BLAST of Gag-Pol polyprotein vs. TrEMBL
Match: A0A4Y2GE16 (Transposon Ty3-I Gag-Pol polyprotein OS=Araneus ventricosus OX=182803 GN=TY3B-I_1495 PE=4 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 2.500e-27
Identity = 67/151 (44.37%), Postives = 88/151 (58.28%), Query Frame = 1 
Query:  172 KPQ*AKQLRPFLGLANYYKRFFKDYXXXXXXXXXXXSGCDKTIKLSENCE*SFNQLKDALTQTPIIDFPRIDWKFILDTDASFEAIGAV*SQIKEDGSDIVIAYS*RHLTAITE----TRKKLFALHEYVLCFRQYFYGKEFVTRTDHKAL 612
            +P+    LR FLGL  YY+RF K+++ +A PLH            +E CE SFN LK ALT +PI+ +PR D  FILDTDAS E IGAV SQ      + VIAY  + L         TRK+L A+ + +  F  Y YG++F+ RTDH +L
Sbjct:  488 RPETVHDLRSFLGLCTYYRRFVKNFSTIAKPLHKLTEA-KSNFNWTEECEKSFNSLKQALTSSPILTYPRTDKDFILDTDASNEGIGAVLSQ-NIGNEERVIAYFSKSLGKPERNYCVTRKELLAIVKSIEHFHHYLYGRKFLLRTDHASL 636

HSP 2 Score: 38.1206 bits (87), Expect = 2.500e-27
Identity = 14/32 (43.75%), Postives = 21/32 (65.62%), Query Frame = 3 
Query:  642 QFQTWVASLSEYNFQLKYRKSEEHANADGLSK 737
            Q   W+  L EY+F++++RK   H NAD LS+
Sbjct:  648 QIARWIQRLQEYDFEIQHRKGTSHGNADALSR 679

HSP 3 Score: 31.187 bits (69), Expect = 2.500e-27
Identity = 16/53 (30.19%), Postives = 31/53 (58.49%), Query Frame = 2 
Query:    2 FNDLIK*FQQICC*LNAFNFADCDLITKSKLKFLGYIISEDKIQSNSEKIKSI 160
             N+L K FQ++       N   C    K ++ +LG++IS + ++++ EKIK++
Sbjct:  432 LNNLRKVFQRLQKATLKLNLKKCRFFQK-EVTYLGHVISAEGVKTDPEKIKAV 483
[Close]
BLAST of Gag-Pol polyprotein vs. Ensembl Cavefish
Match: ENSAMXT00000052614.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001686.1:18497:23810:-1 gene:ENSAMXG00000038033.1 transcript:ENSAMXT00000052614.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 103.605 bits (257), Expect = 2.030e-27
Identity = 61/151 (40.40%), Postives = 95/151 (62.91%), Query Frame = 1 
Query:  172 KPQ*AKQLRPFLGLANYYKRFFKDYXXXXXXXXXXXSGCDKTIKLSENCE*SFNQLKDALTQTPIIDFPRIDWKFILDTDASFEAIGAV*SQIKEDGSDIVIAYS*RHLTAITE----TRKKLFALHEYVLCFRQYFYGKEFVTRTDHKAL 612
            +P    ++R F+GLA YY+RF +D+A +A PLH       +  + +  C+ +F +LK +LT TP++ +PR     ILDTDAS   IGAV SQ+ +DG++ V+AY  R L++  +    TR++L A+ E+   FRQY  G+ F+ R+DH +L
Sbjct:  126 QPTCVSEVRQFVGLAAYYRRFVQDFATIAKPLHELTKKHVR-FQWTPECQAAFEELKSSLTSTPVLGYPRDHGNLILDTDASNFGIGAVLSQV-QDGAERVLAYGSRRLSSTEQNYCTTRRELLAVVEFTRHFRQYLLGRPFIVRSDHSSL 274

HSP 2 Score: 35.039 bits (79), Expect = 2.030e-27
Identity = 15/43 (34.88%), Postives = 23/43 (53.49%), Query Frame = 3 
Query:  642 QFQTWVASLSEYNFQLKYRKSEEHANADGLSK--IGVVCAHNV 764
            Q   W+  L+EY+FQ+ +R    H NAD +S+      C  N+
Sbjct:  286 QLARWLEKLAEYDFQVVHRPGHHHQNADVMSRRPCRTTCPCNM 328

HSP 3 Score: 24.2534 bits (51), Expect = 2.030e-27
Identity = 7/28 (25.00%), Postives = 19/28 (67.86%), Query Frame = 2 
Query:   83 KSKLKFLGYIISEDKIQSNSEKIKSITK 166
            + ++ +LG+I+S   I ++ EK++ + +
Sbjct:   96 RRQVSYLGHIVSAQGIATDPEKVRKVQQ 123

HSP 4 Score: 80.1073 bits (196), Expect = 4.888e-15
Identity = 65/242 (26.86%), Postives = 116/242 (47.93%), Query Frame = 1 
Query: 1321 DRLSKLVSLTATSNQDEKTIWKTLMKNWIYKYGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNGLVERAIITMRDLLVTTMKGGCAEK---QWHELLPRIEFSINATYQSATKYYPFEIVYGRKIT--LNANSGLQNQVQDISKKT----QMNSK-------------QAVKKMREFKDDKRIK*NFEVGEEI--LVCQEPHRRN---KYIIQYDGPYKILRFISEHLIELQ 1965
            D  +K V   A  N    T+ + L   W+ +YG PQT+ +D+G NFE++  ++    LG+ +   T ++ Q +G VER   T++ +L TT     AE+    W  + P    +  AT  S+T   P  +++GR++T  ++  +GL     D         Q+ ++             Q+V++ ++  D   +K  +EVGE +  LV      RN   K++  Y+GPY +L  + + +  +Q
Sbjct:  575 DYFTKWVEAYALPNDQAVTVAEVLTSEWVCRYGAPQTLHSDQGSNFESEVFQKMCELLGVEKTRTTPFRPQSDGQVERFNATLQKILATT-----AERCHWDWDLMTPFAVMAYRATKHSSTGLTPNMMLFGRELTEPIDLVAGLPPDHDDAKTPPEYVIQLRNRLELAHNIAREVLGQSVERAKKQYDKNVLKNRYEVGEAVWHLVKGTKRVRNKVRKFLPSYEGPYFVLGQLDDLVYRIQ 811
[Close]
BLAST of Gag-Pol polyprotein vs. Ensembl Cavefish
Match: ENSAMXT00000048272.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000106.1:219951:223307:1 gene:ENSAMXG00000032559.1 transcript:ENSAMXT00000048272.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 103.605 bits (257), Expect = 2.246e-27
Identity = 61/151 (40.40%), Postives = 95/151 (62.91%), Query Frame = 1 
Query:  172 KPQ*AKQLRPFLGLANYYKRFFKDYXXXXXXXXXXXSGCDKTIKLSENCE*SFNQLKDALTQTPIIDFPRIDWKFILDTDASFEAIGAV*SQIKEDGSDIVIAYS*RHLTAITE----TRKKLFALHEYVLCFRQYFYGKEFVTRTDHKAL 612
            +P    ++R F+GLA YY+RF +D+A +A PLH       +  + +  C+ +F +LK +LT TP++ +PR     ILDTDAS   IGAV SQ+ +DG++ V+AY  R L++  +    TR++L A+ E+   FRQY  G+ F+ R+DH +L
Sbjct:  301 QPTCVSEVRQFVGLAAYYRRFVQDFATIAKPLHELTKKHVR-FQWTPECQTAFEELKSSLTSTPVLGYPRDHGNLILDTDASNFGIGAVLSQV-QDGAERVLAYGSRRLSSTEQNYCTTRRELLAVVEFTRHFRQYLLGRPFIVRSDHSSL 449

HSP 2 Score: 35.039 bits (79), Expect = 2.246e-27
Identity = 15/43 (34.88%), Postives = 23/43 (53.49%), Query Frame = 3 
Query:  642 QFQTWVASLSEYNFQLKYRKSEEHANADGLSK--IGVVCAHNV 764
            Q   W+  L+EY+FQ+ +R    H NAD +S+      C  N+
Sbjct:  461 QLARWLEKLAEYDFQVVHRPGHHHQNADVMSRRPCRTTCPCNM 503

HSP 3 Score: 24.2534 bits (51), Expect = 2.246e-27
Identity = 7/28 (25.00%), Postives = 19/28 (67.86%), Query Frame = 2 
Query:   83 KSKLKFLGYIISEDKIQSNSEKIKSITK 166
            + ++ +LG+I+S   I ++ EK++ + +
Sbjct:  271 RREVSYLGHIVSAQGIATDPEKVRKVQQ 298

HSP 4 Score: 75.0998 bits (183), Expect = 1.685e-13
Identity = 63/244 (25.82%), Postives = 112/244 (45.90%), Query Frame = 1 
Query: 1321 DRLSKLVSLTATSNQDEKTIWKTLMKNWIYKYGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNGLVERAIITMRDLLVTTMKGGCAEK---QWHELLPRIEFSINATYQSATKYYPFEIVYGRKIT--LNANSGLQNQVQDISKKT----QMNSK-------------QAVKKMREFKDDKRIK*NFEVGEEIL-------VCQEPHRRNKYIIQYDGPYKILRFISEHLIELQ 1965
            D  +K V   A  N    T+ + L   W+ +YG PQT+ +D+G NFE++  ++    LG+ +   T ++ Q +G VER   T++ +L TT     AE+    W  + P    +  AT  S+T   P  +++GR++T  ++  +GL     D         Q+  +             Q+V++ ++  D   +K  +EVGE +         C E  +    II  +GPY +L  + + +  +Q
Sbjct:  750 DYFTKWVEAYALPNDQAVTVAEVLTSEWVCRYGAPQTLHSDQGSNFESEVFQKMCELLGVEKTRTTPFRPQSDGQVERFNATLQKILATT-----AERCHWDWDLMTPFAVMAYRATKHSSTGLTPNMMLFGRELTEPIDLVAGLPPDHDDAKTPPEYVIQLRDRLELAHNIAREVLGQSVERAKKQYDKNVLKNRYEVGEAVWHLVKGNQACAEQSQEVPAII--EGPYFVLGQLDDLVYRIQ 986
[Close]
BLAST of Gag-Pol polyprotein vs. Ensembl Cavefish
Match: ENSAMXT00000030446.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000181.1:474500:475675:-1 gene:ENSAMXG00000036877.1 transcript:ENSAMXT00000030446.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 97.4413 bits (241), Expect = 6.336e-25
Identity = 64/150 (42.67%), Postives = 91/150 (60.67%), Query Frame = 1 
Query:  175 PQ*AKQLRPFLGLANYYKRFFKDYXXXXXXXXXXXSGCDKTIKLSENCE*SFNQLKDALTQTPIIDFPRIDWKFILDTDASFEAIGAV*SQIKEDGSDIVIAYS*RHLTAITE----TRKKLFALHEYVLCFRQYFYGKEFVTRTDHKAL 612
            P+ AK +R F+GLA+YY+RF + +A +AAPLH        T + S+  E +F +LK  L   P++ +P +   FI+DTDAS   +GAV SQ+ +DGS+ VIAY  R L         TR++L A+ E +  FR Y YG  F+ RTDH +L
Sbjct:   88 PRNAKMVRSFVGLASYYRRFIRGFADVAAPLHNLTRP-GVTFRWSDEAERAFGELKRRLCNAPVLAYPNMSESFIVDTDASDRGLGAVLSQV-QDGSERVIAYYSRRLDKAERNYCVTRRELLAVVEGLKHFRPYVYGVPFLLRTDHASL 235

HSP 2 Score: 37.3502 bits (85), Expect = 6.336e-25
Identity = 15/45 (33.33%), Postives = 25/45 (55.56%), Query Frame = 3 
Query:  642 QFQTWVASLSEYNFQLKYRKSEEHANADGLSK---IGVVCAHNVR 767
            Q   W++ L E++F++ +R    H NAD LS+   + + C H  R
Sbjct:  247 QLARWISRLQEFSFEVVHRPGRSHGNADALSRRPCVALDCKHCAR 291
[Close]
BLAST of Gag-Pol polyprotein vs. Ensembl Cavefish
Match: ENSAMXT00000058127.1 (pep primary_assembly:Astyanax_mexicanus-2.0:16:35454045:35455481:1 gene:ENSAMXG00000038477.1 transcript:ENSAMXT00000058127.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 108.612 bits (270), Expect = 1.025e-24
Identity = 87/311 (27.97%), Postives = 150/311 (48.23%), Query Frame = 1 
Query: 1111 RREFLLAKM*ETVKDLLKRCVTCAKRKID*GKTKEILIPRESSEFLEHIFMHV-AVMERXXXXXXXXXXXXDRLSKLVSLTATSNQDEKTIWKTLMKNWIYKYGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNGLVE---RAIITMRDLLVTTMKGGCAEKQWHELLPRIEFSINATYQSATKYYPFEIVYGRKITLNA----NSGLQNQVQD----ISKKTQMNSK--QAVKKMREFKDDKRIK*NFEV---------GEEILVCQEPHRRN---KYIIQYDGPYKILRFISEHLIELQ 1965
            R  F      E V+   + C  CA RK      +  L+P  +S   E I + +   +  T    +Y++V+ D  SK     +  NQ+ KT+ K L + WI +YG P++I TD+GRNFE+    E    L + +   + Y+ Q +GL+E   R +++M  L V        ++ W  LLP +  +  ++  ++T + P+ +++G +I L      N+G+Q + Q     +S+   + S   +AVKK  + +  +  K NF+V         GE + V  E  +R    K   +Y GPY++L  +S+ L  +Q
Sbjct:   28 RTRFYWPGWVEDVERWCRECTDCASRKTSGPAPRAPLLPSVTSRPYERIALDILGPLPETPQKNRYILVVGDYFSKWTEAFSLPNQEAKTVAKVLTEEWICRYGAPRSIHTDQGRNFESHLFSELCRLLNMHKSRTSPYRPQSDGLIERFNRTLLSMLSLFVDA-----NQQDWDALLPFVMMAYRSSVHASTGFTPYRVLFGHEIVLPVDVLLNTGVQEKFQTTNEYVSRMEGILSTVCEAVKK-HQIRASEGQKQNFDVKVNFQYYSEGELVWVKNEARKRGVCPKLQRRYRGPYRVLEKLSDVLYRIQ 332
[Close]
BLAST of Gag-Pol polyprotein vs. Ensembl Cavefish
Match: ENSAMXT00000031920.1 (pep primary_assembly:Astyanax_mexicanus-2.0:12:11094903:11101818:1 gene:ENSAMXG00000039354.1 transcript:ENSAMXT00000031920.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 105.531 bits (262), Expect = 2.950e-24
Identity = 83/294 (28.23%), Postives = 145/294 (49.32%), Query Frame = 1 
Query: 1162 KRCVTCAKRKID*GKTKEILIPRESSEFLEHIFMHV-AVMERXXXXXXXXXXXXDRLSKLVSLTATSNQDEKTIWKTLMKNWIYKYGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNGLVE---RAIITMRDLLVTTMKGGCAEKQWHELLPRIEFSINATYQSATKYYPFEIVYGRKITLNA----NSGLQNQVQD----ISKKTQMNSK--QAVKKMREFKDDKRIK*NFEV---------GEEILVCQEPHRRN---KYIIQYDGPYKILRFISEHLIELQ 1965
            + C  CA RK      +  L+P  +S   E I + +   +  T    +Y++V+ D  SK     +  NQ+ KT+ K L + WI +YG P++I TD+GRNFE+    E    L + +   + Y+ Q +GL+E   R +++M  L V        ++ W  LLP +  +  ++  ++T + P+ +++G +I L      N+G+Q + Q     +S+   + S   +AVKK  + +  +  K NF+V         GE + V  E  +R    K   +Y GPY++L  +S+ L  +Q
Sbjct:    9 RECTDCASRKTSGPAPRAPLLPSVTSRPYERIALDILGPLPETPQKNRYILVVGDYFSKWTEAFSLPNQEAKTVAKVLTEEWICRYGAPRSIHTDQGRNFESHLFSELCRLLNMHKSRTSPYRPQSDGLIERFNRTLLSMLSLFVDA-----NQQDWDALLPFVMMAYRSSVHASTGFTPYRVLFGHEIVLPVDVLLNTGVQEKFQTTNEYVSRMEGILSTVCEAVKK-HQIRASEGQKQNFDVKVNFQYYSEGELVWVKNEARKRGVCPKLQRRYRGPYRVLEKLSDVLYRIQ 296
[Close]
BLAST of Gag-Pol polyprotein vs. Ensembl Medaka
Match: ENSORLT00000040305.1 (pep primary_assembly:ASM223467v1:1:25459511:25466649:-1 gene:ENSORLG00000028409.1 transcript:ENSORLT00000040305.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 96.2857 bits (238), Expect = 3.945e-24
Identity = 60/150 (40.00%), Postives = 90/150 (60.00%), Query Frame = 1 
Query:  175 PQ*AKQLRPFLGLANYYKRFFKDYXXXXXXXXXXXSGCDKTIKLSENCE*SFNQLKDALTQTPIIDFPRIDWKFILDTDASFEAIGAV*SQIKEDGSDIVIAYS*RHLTAITE----TRKKLFALHEYVLCFRQYFYGKEFVTRTDHKAL 612
            P+   ++R F+GLA+YY+RF  D+A +A PLH       +    +  C+ +F +LK+ LT  PI+ +P    + +LDTDAS   +GAV SQ+ + G + V+AY  R LT   +    TR++L A+ E+   FRQY  G+ FV RTDH +L
Sbjct: 1056 PKNISEVRQFVGLASYYRRFVADFATIARPLHELTKKYAR-FDWTTECQEAFEELKERLTSAPILGYPLDSGELLLDTDASDWGVGAVLSQV-QGGEERVLAYGSRRLTTTEQNYCTTRRELLAVVEFTSHFRQYLLGRSFVLRTDHSSL 1203

HSP 2 Score: 35.8094 bits (81), Expect = 3.945e-24
Identity = 16/40 (40.00%), Postives = 23/40 (57.50%), Query Frame = 3 
Query:  618 TTKKSISPQFQTWVASLSEYNFQLKYRKSEEHANADGLSK 737
            T  K    Q   W+  L+EY+FQ+ +R  + H NAD LS+
Sbjct: 1207 TRLKEPEGQLARWLEKLAEYDFQVLHRPGKVHQNADALSR 1246

HSP 3 Score: 72.0182 bits (175), Expect = 1.579e-12
Identity = 59/243 (24.28%), Postives = 113/243 (46.50%), Query Frame = 1 
Query: 1321 DRLSKLVSLTATSNQDEKTIWKTLMKNWIYKYGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNGLVERAIITMRDLLVTTMKGGCAEK---QWHELLPRIEFSINATYQSATKYYPFEIVYGRKIT--LNANSGLQNQ----------VQDISKKTQMNSK-------QAVKKMREFKDDKRIK*NFEVGEEILVCQEPHRR-----NKYIIQYDGPYKILRFISEHLIELQY 1968
            D  SK V      N+   T+ + ++  W+ +YG P  + +D+G NFE+   +     LGI +   T ++ Q +G VER   T++  L  T     AE+    W  ++P       AT  S+T   P  +++GR+IT  ++   GL  +          VQ + ++ +++ +       +AV++ +   D    +  +++G+ +    +  +R      K++  Y+GPY    F+ +HL +L Y
Sbjct: 1507 DYFSKWVEAYPVPNEQATTVAEKIVSEWVCRYGAPYELHSDQGANFESAVFQGMCELLGINKTRTTPFRPQSDGQVERFNATLQKTLAAT-----AERCHWDWDIMIPYALMPYRATKHSSTGLTPNMMLFGREITEPMDLVVGLPPENLTVDTAPEYVQRLRQRLELSHQLARSVLGRAVERAKRQYDKNICQVQYKIGDAVWYLLKGTKRVKNKVRKFLPSYEGPY----FVVDHLDDLVY 1740
[Close]
BLAST of Gag-Pol polyprotein vs. Ensembl Medaka
Match: ENSORLT00000036827.1 (pep primary_assembly:ASM223467v1:16:28613823:28617539:1 gene:ENSORLG00000023550.1 transcript:ENSORLT00000036827.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 84.7297 bits (208), Expect = 1.019e-20
Identity = 61/147 (41.50%), Postives = 86/147 (58.50%), Query Frame = 1 
Query:  187 KQLRPFLGLANYYKRFFKDYXXXXXXX-XXXXSGCDKTIKLSENCE*SFNQLKDALTQTPIIDFPRIDWKFILDTDASFEAIGAV*SQIKEDGSDIVIAYS*RHLTAITE----TRKKLFALHEYVLCFRQYFYGKEFVTRTDHKAL 612
            + L+ FLGLA+YY+RF + ++ +AAPL H     CD     ++ CE +F+ LK ALT +PI+  P     FILDTDAS   +GAV SQ+   G + V+AY  + L+        TR++L A+ + +  FR Y  G  F  RTDH AL
Sbjct:  501 RDLKSFLGLASYYRRFVRGFSCIAAPLFHLQRKDCD--FVWTQECEQAFSSLKKALTNSPILTPPDPKLPFILDTDASDVGMGAVLSQMGSAG-ERVVAYFSKTLSKAERRYCVTRRELLAVVKAIGHFRYYLCGLPFTVRTDHSAL 644

HSP 2 Score: 33.4982 bits (75), Expect = 1.019e-20
Identity = 11/32 (34.38%), Postives = 20/32 (62.50%), Query Frame = 3 
Query:  642 QFQTWVASLSEYNFQLKYRKSEEHANADGLSK 737
            Q   W+  L+ ++F +++R    HANAD +S+
Sbjct:  656 QIARWLEELASFSFTVEHRPGSRHANADAMSR 687

HSP 3 Score: 21.9422 bits (45), Expect = 1.019e-20
Identity = 8/26 (30.77%), Postives = 18/26 (69.23%), Query Frame = 2 
Query:   83 KSKLKFLGYIISEDKIQSNSEKIKSI 160
            + +L+FLG+ I  + I +  EK++++
Sbjct:  466 RRELEFLGHKIGGEGISTLEEKVQAV 491

HSP 4 Score: 64.3142 bits (155), Expect = 3.823e-10
Identity = 72/312 (23.08%), Postives = 140/312 (44.87%), Query Frame = 1 
Query: 1111 RREFLLAKM*ETVKDLLKRCVTCAKRKID*GKTKEILIPRESSEFLEHIFMHV-AVMERXXXXXXXXXXXXDRLSKLVSLTATSNQDEKTIWKTLMKNWIYKYGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNGLVERAIITM-RDLLVTTMKGGCAEKQWHELLPRIEFSINATYQSATKYYPFEIVYGRKI-------------TLNANSGLQ--NQVQDISKKTQMNSKQAVKK--MREFKD-DKRIK-*NFEVGEEILVCQEPHRRN----KYIIQYDGPYKILRFISEHLIELQYP 1971
            R+ F   ++   V+D  +RC  C   K    +++  L    +   +E + + +     RT    ++V+V +D  +K     A  +Q+  T+   L++    ++G  + I +D+GRNFE+        +LG+R+   T    Q +GLVER   T+ + L + T      +  W E LP +  +  +  Q +T   P  ++ GR++              L A  G +   ++QD        ++  ++K  +R+ ++ D R K  +F+ G+ + V   P R+     K   Q+ GP ++L  + E +  ++ P
Sbjct:  836 RQGFYWGQLRRDVEDFCRRCDICTAHKGPPDRSRAELQQLAAGAPMERVAVDIMGPFPRTNRGNRFVLVAMDYFTKWPEAYAIPDQEAVTVADALVEGMFSRFGAAEVIHSDQGRNFESAVFSAMCERLGMRKTRTTPLHPQSDGLVERFNRTLVKQLAILT---SAHQSDWDEHLPLVLMAYRSAVQDSTLCTPALLMLGRELRTPAEMSFGKPPDALGAPPGPEYARKLQDRMDTAHAFARNQLEKAGIRQKRNYDLRAKGKDFKAGDLVWV-YNPKRKKGRCPKLDCQWVGPCEVLEKLGEVVYRVELP 1143
[Close]
BLAST of Gag-Pol polyprotein vs. Ensembl Medaka
Match: ENSORLT00000045141.1 (pep primary_assembly:ASM223467v1:23:22495254:22500491:-1 gene:ENSORLG00000029628.1 transcript:ENSORLT00000045141.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 70.0922 bits (170), Expect = 1.513e-19
Identity = 55/162 (33.95%), Postives = 81/162 (50.00%), Query Frame = 1 
Query:  175 PQ*AKQLRPFLGLANYYKRFFKDYXXXXXXXXXXXSGCDKTIKLSEN--------CE*SFNQLKDALTQTPIIDFPRIDWKFILDTDASFEAIGAV*SQIKEDGSDIVIAYS*RHLTAITETRKKLFALHEYVLC--------FRQYFYGKEFVTRTDHKAL 612
            P+  K++R  +G  +YY+RF  ++A +A PLHA      K  K++EN        C+ + ++LK  LT  P++ +P     FIL TD S   +GAV SQ K+DG + V+AY+ R L    +  K   A    +L         FR Y    +F   TDH  L
Sbjct:  943 PRSVKEVRQVVGFMSYYRRFVPNFAHMAKPLHALLG---KRGKVNENQPFVWTADCQTALDELKQCLTSPPVLAYPDFQTPFILTTDGSSHGLGAVLSQ-KQDGVERVVAYASRGLRGSEKNDKYYSAFKLELLALKWAITEKFRDYLMFSKFTVVTDHNPL 1100

HSP 2 Score: 39.2762 bits (90), Expect = 1.513e-19
Identity = 16/31 (51.61%), Postives = 23/31 (74.19%), Query Frame = 3 
Query:  648 QTWVASLSEYNFQLKYRKSEEHANADGLSKI 740
            Q WVA L+EYNF++ Y+   ++ NAD LS+I
Sbjct: 1113 QRWVAQLAEYNFEVCYKPGRQNINADVLSRI 1143

HSP 3 Score: 26.5646 bits (57), Expect = 1.513e-19
Identity = 10/30 (33.33%), Postives = 21/30 (70.00%), Query Frame = 2 
Query:   74 LITKSKLKFLGYIISEDKIQSNSEKIKSIT 163
             + + ++KFLG++IS   I+ + EK+ ++T
Sbjct:  909 FLLRPEVKFLGHLISAQGIKVDMEKVSALT 938

HSP 4 Score: 91.6633 bits (226), Expect = 1.535e-18
Identity = 84/301 (27.91%), Postives = 131/301 (43.52%), Query Frame = 1 
Query: 1096 TVSLPRREFLLAKM*ETVKDLLKRCVTCAKRKID*GKTKEILIPRESSEFLEHIFMHVAVMERXXXXXXXXXXXXDRLSKLVSLTATSNQDEKTIWKTLMKNWIYKYGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNGLVERAIITMRDLLVTTMKGGCAEKQWHELLPRIEFSINATYQSATKYYPFEIVYGR--KITLNANSGLQNQVQDISKKTQMNSKQAVKKMREFKDDKRIK*NFEVGEEILVCQEPHRRNKYIIQYDGPYKILRFISEHLIELQYPTQRQ*IE 1992
            T+SL RR +      + V+  +++C  CA  K    K +  +     +  LE + M   ++ER+    + V+V+ D  ++      T NQ   T  K L+K+W   YG P  + +D+GR+FE   +KE     GI +   + Y  Q N   ER   TM D+L T       ++ W   LP +  + N    S+T Y PF +++GR  ++ L+   G     +D+++    N    VK   E     R+K   EV    L  Q   RR K I        +LR     LI    P  R  I+
Sbjct: 1297 TLSLLRRSYYWPSTGQDVQSWVQQCKRCALAKDVFPKARAPMTCSNVTAPLEVVAMDYTLLERSVGGYENVLVLTDMFTRFTMAVPTKNQTADTTAKALVKHWFAYYGCPARLHSDQGRSFEASVIKELCKIYGIAKSRTSPYHPQGNAQCERFNRTMHDMLRTLPPE--KKRDWKAYLPELSMAYNNRVHSSTGYSPFYLMFGRDARMPLDLLGG-----KDLAEVDIDNLDDWVKAHHE-----RLKLAVEVAG--LSAQGASRRQKRIYDRSSCSALLRSGDRVLIRNHKPRGRNKIQ 1583
[Close]
BLAST of Gag-Pol polyprotein vs. Ensembl Medaka
Match: ENSORLT00000037716.1 (pep primary_assembly:ASM223467v1:20:9739400:9742664:-1 gene:ENSORLG00000027308.1 transcript:ENSORLT00000037716.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 73.9442 bits (180), Expect = 1.935e-19
Identity = 57/161 (35.40%), Postives = 85/161 (52.80%), Query Frame = 1 
Query:  175 PQ*AKQLRPFLGLANYYKRFFKDYXXXXXXXXXXXSGCDKT-------IKLSENCE*SFNQLKDALTQTPIIDFPRIDWKFILDTDASFEAIGAV*SQIKEDGSDIVIAYS*RHLTAITET-------RKKLFALHEYVL-CFRQYFYGKEFVTRTDHKAL 612
            P   K +R FLGLA YY+RF   +A +A PL++   G   T       I  + +C+ SF+ LK+ALTQ PI+ +   +  F++ TDAS   +GAV +Q++E G + VIAY+ R L             + +L AL   +   F+ Y  G +F   TD+  L
Sbjct:  343 PSTIKGVRAFLGLAGYYRRFVAGFANIARPLNSLLVGIPATKRSGTQRIVWTPDCKASFDALKEALTQAPILAYADFNKPFVVYTDASHHGLGAVLAQVQE-GRERVIAYASRSLHPSERNDANYSSFKLELLALKWAITEKFKDYLMGAKFTVFTDNNPL 502

HSP 2 Score: 35.4242 bits (80), Expect = 1.935e-19
Identity = 13/30 (43.33%), Postives = 21/30 (70.00%), Query Frame = 3 
Query:  648 QTWVASLSEYNFQLKYRKSEEHANADGLSK 737
            Q WVA L+ +++ +KYR  + + NAD LS+
Sbjct:  515 QRWVAQLASFDYDIKYRSGKNNTNADALSR 544

HSP 3 Score: 26.5646 bits (57), Expect = 1.935e-19
Identity = 8/26 (30.77%), Postives = 19/26 (73.08%), Query Frame = 2 
Query:   83 KSKLKFLGYIISEDKIQSNSEKIKSI 160
            + ++KFLG+I+    ++ + EK+K++
Sbjct:  312 QQEVKFLGHIVDRSGVRPDPEKVKAV 337
[Close]
BLAST of Gag-Pol polyprotein vs. Ensembl Medaka
Match: ENSORLT00000038517.1 (pep primary_assembly:ASM223467v1:24:17777464:17778898:-1 gene:ENSORLG00000028198.1 transcript:ENSORLT00000038517.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 88.1965 bits (217), Expect = 4.048e-18
Identity = 65/293 (22.18%), Postives = 135/293 (46.08%), Query Frame = 1 
Query: 1135 M*ETVKDLLKRCVTCAKRKID*GKTKEILIPRESSEFLEHIFMHVAVMERXXXXXXXXXXXXDRLSKLVSLTATSNQDEKTIWKTLMKNWIYKYGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNGLVERAIITMRDLLVTTM--KGGCAEKQWHELLPRIEFSINATYQSATKYYPFEIVYGRKITLNAN--------------------SGLQNQVQDISKKTQMNSKQAVKKMREFKDDKRIK*NFEVGEEILVCQEPHRRNKYIIQYDGPYKILRFISE 1947
            M + ++   ++C  C  R+    K K  +        L+ +  H+  +  T    +YV+V+ D  +K V++ A  NQ  +T+ + L ++++  +G P+ + +D+GR FE + ++     LGI +    +Y  + +G+VER   T+ D L   +   GG    +W + L  + F+ N +  ++T++ PF +++GR+  + A                     S L  Q++      ++N+ +A +K + + D+      F  G  + +      R K    + GPY++ R ++ 
Sbjct:    1 MLKDIRQWCEQCRACQTRRSPVPKAKAPMGGSPVCRPLQRVAAHILELPLTSRGHRYVLVVEDYFTKFVNVYALPNQTAETVARCLFEDYVLVHGVPEVLHSDQGRQFEAEVIQNLCRLLGIAKTRTAAYNPKSDGMVERHNRTLIDQLAKMLLSHGG----EWDDHLKSVAFAYNTSKHTSTRFTPFYLMHGREARIPAEVLIPSGVGGIGSAATLPLYASSLVEQLEIAFSAARVNAAEAQEKQKLYHDENSHHKGFTEGALVWLNNPTEGRTKLAPHWKGPYRVDRVLAS 289
[Close]
BLAST of Gag-Pol polyprotein vs. Planmine SMEST
Match: SMESG000015193.1 (SMESG000015193.1)

HSP 1 Score: 315.464 bits (807), Expect = 6.550e-102
Identity = 188/320 (58.75%), Postives = 217/320 (67.81%), Query Frame = 1 
Query: 1114 REFLLAKM*ETVKDLLKRCVTCAKRKID*GKTKEILIPRESSEFLEHIFMHVAVMERXXXXXXXXXXXXDRLSKLVSLTATSNQDEKTIWKTLMKNWIYKYGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNGLVERAIITMRDLLVTTMKGGCAEKQWHELLPRIEFSINATYQSATKYYPFEIVYGRKITLNANSGLQNQVQDISKKTQMNSKQAVKKMREFKDDKRIK*NFEVGEEILVCQEPHRRNKYIIQYDGPYKILRFISEHLIELQYPT--QRQ*IECLKRWHQS*KVKSNDSTFKVKDQMF 2067
            + F   KM ETVKD+++RCV CAKRKID GKTKEILI RESSE LE I M VAVMERT T K YVIVII R SKL                TLM NWIYKYGKPQ+ILTDRG NFE+KYLKEKLGQLGIRQEFA+ YQHQ NGLVER I TMRDL V             ++L  +  +  +            I Y R +     S  QNQ+QDIS+KTQMNSKQ+V+KM E +DDKRIK NF+VGEE+LV +EPHRRNK  IQYDGPYKILRFISEH +E QYP   + + IE LK+WH   + +S+D T  VKDQ+ 
Sbjct:   39 KNFFWPKMQETVKDIVQRCVKCAKRKIDQGKTKEILILRESSECLEQIVMDVAVMERTSTEKMYVIVIIHRFSKL----------------TLMNNWIYKYGKPQSILTDRGGNFESKYLKEKLGQLGIRQEFASPYQHQSNGLVERTIRTMRDLHVA-----------RDVLKTVARTAASNL----------IQYKRNL-----SKFQNQIQDISEKTQMNSKQSVQKMSELEDDKRIKRNFDVGEEVLVRREPHRRNKNDIQYDGPYKILRFISEHQVEFQYPNTMRHRRIEWLKQWHHFQEGESDDITLNVKDQIL 316

HSP 2 Score: 84.7297 bits (208), Expect = 1.385e-17
Identity = 39/51 (76.47%), Postives = 44/51 (86.27%), Query Frame = 2 
Query: 1001 MD*IDLVVIPKSYQSKLVIKIHEDLCHIGIKKLFHYLEGNFFWQKCKKLLK 1153
            MD ID+VVIPKSYQSKLVIK HE LCH+GIKKLFHYLE NFFW K ++ +K
Sbjct:    1 MDKIDIVVIPKSYQSKLVIKTHEGLCHVGIKKLFHYLEKNFFWPKMQETVK 51
[Close]
BLAST of Gag-Pol polyprotein vs. Planmine SMEST
Match: SMESG000045039.1 (SMESG000045039.1)

HSP 1 Score: 296.204 bits (757), Expect = 3.064e-96
Identity = 157/198 (79.29%), Postives = 172/198 (86.87%), Query Frame = 1 
Query: 1276 MERXXXXXXXXXXXXDRLSKLVSLTATSNQDEKTIWKTLMKNWIYKYGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNGLVERAIITMRDLLVTTMKGGCAEKQWHELLPRIEFSINATYQSATKYYPFEIVYGRKITLNANSGLQNQVQDISKKTQMNSKQAVKKMREFKDDKRIK*NFEVGEEILV 1869
            MER  T KKYVIVII R SKLVSLTAT NQDEKTIWK LM NWIYKY KPQ+IL DRGRNFE+KYLKEKL QLGIRQE+AT YQHQ NG+VE A  TMRDLLVTTMK GCA+KQWHELLP+ EFSIN TYQSATKY PFEIV+GRKITL  NS LQNQVQD+S+KTQMNSKQAVKK+REF+DDK IK NFE+ +++LV
Sbjct:    1 MERISTDKKYVIVIIGRFSKLVSLTATPNQDEKTIWKRLMNNWIYKYCKPQSILMDRGRNFESKYLKEKLRQLGIRQEYATPYQHQSNGIVEIANRTMRDLLVTTMKIGCADKQWHELLPQTEFSINPTYQSATKYSPFEIVHGRKITLYVNSRLQNQVQDVSEKTQMNSKQAVKKIREFEDDKGIKKNFEMRDDVLV 198
[Close]
BLAST of Gag-Pol polyprotein vs. Planmine SMEST
Match: SMESG000073986.1 (SMESG000073986.1)

HSP 1 Score: 206.453 bits (524), Expect = 1.888e-84
Identity = 115/244 (47.13%), Postives = 160/244 (65.57%), Query Frame = 1 
Query: 1159 LKRCVTCAKRKID*GKTKEILIPRESSEFLEHIFMHVAVMERXXXXXXXXXXXXDRLSKLVSLTATSNQDEKTIWKTLMKNWIYKYGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNGLVERAIITMRDLLVTTMKGGCAEKQWHELLPRIEFSINATYQSATKYYPFEIVYGRKITLNANSG-LQNQVQDISKKTQMNSKQAVKKMREFKDDKRIK*NFEVGEEILVCQEPHR 1887
            +K+C   A RKID G+ KEIL+PR    FLE I + +A ME T   K  +IVII+R SKLVSL+A S QDE TI   ++ NWIY++G+P++ILTDRGR FE     + + + GI+QEF++ YQHQ NGL ER I T+RD+L T++     +  W  LLP+IEFS+NAT + +TK+ PFEI+Y RKI L +  G +Q   ++I  +T+ N  +A   M+    D +    F VG+++LV  EPHR
Sbjct:  331 IKKCKIYASRKIDQGRAKEILLPRTRKRFLEQIVVDIAYME-TKESKNCMIVIINRFSKLVSLSAASTQDEATILNVILNNWIYRFGRPESILTDRGRIFEGSMFHDWMEKFGIKQEFSSPYQHQSNGLAERIIRTVRDMLATSLAKIKTKNNWCILLPKIEFSLNATIKISTKFSPFEIIYWRKINLYSGVGHIQKFREEIEDETKTNLVKAATTMKNRDLDNQGTRVFMVGKKVLVRLEPHR 573

HSP 2 Score: 82.0333 bits (201), Expect = 1.888e-84
Identity = 34/51 (66.67%), Postives = 45/51 (88.24%), Query Frame = 3 
Query:  609 FMNTTKKSISPQFQTWVASLSEYNFQLKYRKSEEHANADGLSKI-GVVCAH 758
            FMNTTKK I+PQFQTW+A+LSEY+F L+YRK+EEH NADG+S++   +C+H
Sbjct:  181 FMNTTKKPINPQFQTWMANLSEYDFALQYRKAEEHGNADGMSRLNNTICSH 231

HSP 3 Score: 51.9878 bits (123), Expect = 1.888e-84
Identity = 36/107 (33.64%), Postives = 60/107 (56.07%), Query Frame = 2 
Query:  746 SLCAQCQTAYIQHKQEKPKVRYINLIQKTQTMIEIMEE-QKKDKLFKEA-MEILRNEIGV*NESFQNSFLFKYKDQLKKSDDMLVIEMD*IDLVVIPKSYQSKLVIK 1060
            ++C+ CQ      K+ K + RYIN +Q +  +I+I+++ Q KD++     + +  NE  +  E+  +S +FKY   L+  DD+L+I  D    VV+P SY   L IK
Sbjct:  227 TICSHCQMERKDSKRAKCRTRYINSLQGSSNIIKIIKQKQNKDRVTSVIILHLNGNEAHISYETISSS-IFKYSKILQIQDDVLMINTDGKLAVVVPDSYAKSLCIK 332

HSP 4 Score: 35.8094 bits (81), Expect = 1.888e-84
Identity = 14/19 (73.68%), Postives = 16/19 (84.21%), Query Frame = 1 
Query:  556 FRQYFYGKEFVTRTDHKAL 612
            FRQY YG+ FV +TDHKAL
Sbjct:  161 FRQYKYGRRFVAKTDHKAL 179
[Close]
BLAST of Gag-Pol polyprotein vs. Planmine SMEST
Match: SMESG000080594.1 (SMESG000080594.1)

HSP 1 Score: 262.307 bits (669), Expect = 2.493e-82
Identity = 147/219 (67.12%), Postives = 164/219 (74.89%), Query Frame = 1 
Query: 1246 LEHIFMHVAVMERXXXXXXXXXXXXDRLSKLVSLTATSNQDEKTIWKTLMKNWIYKYGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNGLVERAIITMRDLLVTTMKGGCAEKQWHELLPRIEFSINATYQSATKYYPFEIVYGRKITLNANSGLQNQVQDISKKTQMNSKQAVKKMREFKDDKRIK*NFEVGEEILVCQEPHRRNKYI 1902
            L+ I ++VAVMERT T KKYV+VIIDR S+LVS+TAT NQ EKTIWKTLM NWIYKYGKPQ+ILT                           YQHQ NGLVERAI TMR+L+V TMK GCAEKQWHELL RIEFSI ATYQSATKY  FEIVYGRK+TL+ANS LQNQV+DIS+KTQMNSKQ VK M+EFKD+  IK  FEVGEE+LV   PHR +K I
Sbjct:   45 LKQIVINVAVMERTSTDKKYVMVIIDRFSELVSITATPNQYEKTIWKTLMNNWIYKYGKPQSILTP--------------------------YQHQSNGLVERAIRTMRNLVVITMKVGCAEKQWHELLTRIEFSIKATYQSATKYLLFEIVYGRKLTLHANSRLQNQVRDISEKTQMNSKQTVKNMKEFKDNNSIKRKFEVGEEVLVLLVPHRISKII 237
[Close]
BLAST of Gag-Pol polyprotein vs. Planmine SMEST
Match: SMESG000018547.1 (SMESG000018547.1)

HSP 1 Score: 240.35 bits (612), Expect = 6.092e-73
Identity = 137/301 (45.51%), Postives = 196/301 (65.12%), Query Frame = 1 
Query: 1120 FLLAKM*ETVKDLLKRCVTCAKRKID*GKTKEILIPRESSEFLEHIFMHVAVMERXXXXXXXXXXXXDRLSKLVSLTATSNQDEKTIWKTLMKNWIYKYGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNGLVERAIITMRDLLVTTMKGGCAEKQWHELLPRIEFSINATYQSATKYYPFEIVYGRKITLNANSGLQNQVQ-DISKKTQMNSKQAVKKMREFKDDKRIK*NFEVGEEILVCQEPHRRNKYIIQYDGPYKILRFISEHLIELQYPT---QRQ*IECLKRWH 2010
            F    + +++++ L++C  CAKRKID  + KEI + R SSEFL+ I   +A M ++   K  V++I DR SKLVSLT  S QD++TI++ +M N IY++GKP +ILT++G+ FE+ + KE L +LGI+QE ++ YQHQ NGLVER I TM DL+ TT+ G C EK W ELL +IEF INAT QS+T    FE ++G +I L+     + + Q +I+   + N+ +A  +M+     KR    FEVGE+ LV +EP  R K  +QY+  YKI++FIS H +E Q  T   QR+ IE LK+W 
Sbjct:   43 FFWPSIQDSIQECLRKCAECAKRKIDQKEIKEIFLQRGSSEFLKQIV--IAYMNQSVEKKYVVVII-DRFSKLVSLTVASKQDDQTIFRIIMNNLIYRFGKPISILTEKGKCFESLFFKESLSKLGIKQELSSPYQHQSNGLVERVIRTMTDLITTTLAGECNEKHWVELLTKIEFMINATQQSSTGLSQFENIFGTQINLHFTLQPKPESQENINMGVKFNAGKAAVRMKNMDGSKRGSRLFEVGEDFLVIKEPQNRKKDELQYEDQYKIIKFISPHQVEFQIGTTVKQRR-IEWLKKWQ 339
The following BLAST results are available for this feature:
BLAST of Gag-Pol polyprotein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Gag-Pol polyprotein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Gag-Pol polyprotein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Gag-Pol polyprotein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
FO704673.14.177e-1425.24pep chromosome:GRCz11:12:14545475:14551077:-1 gene... [more]
CABZ01030006.13.027e-1224.74pep scaffold:GRCz11:KN150258.1:33006:36502:-1 gene... [more]
CR354395.21.776e-1033.33pep chromosome:GRCz11:12:13553730:13556536:-1 gene... [more]
CR533578.31.912e-1033.33pep chromosome:GRCz11:16:23466094:23471387:-1 gene... [more]
BX571665.11.927e-1033.33pep chromosome:GRCz11:17:35431724:35437222:1 gene:... [more]
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BLAST of Gag-Pol polyprotein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
anxa65.038e-2035.76annexin A6 [Source:Xenbase;Acc:XB-GENE-989741][more]
ENSXETT00000024810.17.974e-1733.54pep primary_assembly:Xenopus_tropicalis_v9.1:KV464... [more]
ENSXETT00000008059.13.174e-1524.04pep primary_assembly:Xenopus_tropicalis_v9.1:3:106... [more]
npm18.725e-1524.04nucleophosmin (nucleolar phosphoprotein B23, numat... [more]
mknk29.648e-1523.72MAPK interacting serine/threonine kinase 2 [Source... [more]
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BLAST of Gag-Pol polyprotein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Gag-Pol polyprotein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P20825|POL2_DROME4.926e-1635.81Retrovirus-related Pol polyprotein from transposon... [more]
sp|P04323|POL3_DROME2.329e-1532.43Retrovirus-related Pol polyprotein from transposon... [more]
sp|P10394|POL4_DROME1.402e-1432.24Retrovirus-related Pol polyprotein from transposon... [more]
sp|Q8I7P9|POL5_DROME2.245e-1337.89Retrovirus-related Pol polyprotein from transposon... [more]
sp|Q87040|POL_SFVCP9.161e-1025.34Pro-Pol polyprotein OS=Simian foamy virus (isolate... [more]
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BLAST of Gag-Pol polyprotein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3B3DB171.084e-4039.26Uncharacterized protein OS=Oryzias melastigma OX=3... [more]
A0A4Y2GDB09.271e-2844.37Transposon Ty3-I Gag-Pol polyprotein OS=Araneus ve... [more]
Q94BM21.196e-2729.00Gag-pol polyprotein OS=Hordeum vulgare OX=4513 PE=... [more]
A0A4Y2GA361.361e-2744.37Transposon Ty3-I Gag-Pol polyprotein OS=Araneus ve... [more]
A0A4Y2GE162.500e-2744.37Transposon Ty3-I Gag-Pol polyprotein OS=Araneus ve... [more]
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BLAST of Gag-Pol polyprotein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000052614.12.030e-2740.40pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000048272.12.246e-2740.40pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000030446.16.336e-2542.67pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000058127.11.025e-2427.97pep primary_assembly:Astyanax_mexicanus-2.0:16:354... [more]
ENSAMXT00000031920.12.950e-2428.23pep primary_assembly:Astyanax_mexicanus-2.0:12:110... [more]
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BLAST of Gag-Pol polyprotein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Gag-Pol polyprotein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Gag-Pol polyprotein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Gag-Pol polyprotein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000040305.13.945e-2440.00pep primary_assembly:ASM223467v1:1:25459511:254666... [more]
ENSORLT00000036827.11.019e-2041.50pep primary_assembly:ASM223467v1:16:28613823:28617... [more]
ENSORLT00000045141.11.513e-1933.95pep primary_assembly:ASM223467v1:23:22495254:22500... [more]
ENSORLT00000037716.11.935e-1935.40pep primary_assembly:ASM223467v1:20:9739400:974266... [more]
ENSORLT00000038517.14.048e-1822.18pep primary_assembly:ASM223467v1:24:17777464:17778... [more]
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BLAST of Gag-Pol polyprotein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000015193.16.550e-10258.75SMESG000015193.1[more]
SMESG000045039.13.064e-9679.29SMESG000045039.1[more]
SMESG000073986.11.888e-8447.13SMESG000073986.1[more]
SMESG000080594.12.493e-8267.12SMESG000080594.1[more]
SMESG000018547.16.092e-7345.51SMESG000018547.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031648 ID=SMED30031648|Name=Gag-Pol polyprotein|organism=Schmidtea mediterranea sexual|type=transcript|length=2124bp
ATTTAATGATTTAATTAAATAATTTCAACAAATCTGTTGCTGATTGAACG
CTTTTAATTTTGCCGATTGTGATTTAATCACCAAATCCAAATTAAAATTT
CTTGGCTATATTATCAGTGAAGACAAAATACAGTCAAATTCCGAGAAGAT
CAAGTCAATTACAAAAGGACAAAACCGCAGTGAGCAAAACAACTTCGACC
ATTTCTGGGTCTGGCGAATTACTATAAACGATTTTTCAAAGATTATGCAA
AATTAGCAGCACCTTTGCATGCAGCAGCTTCAGGCTGTGATAAAACAATC
AAATTGTCAGAAAATTGTGAATAAAGCTTTAATCAACTTAAAGATGCTCT
AACACAAACTCCTATTATTGATTTCCCTCGAATAGATTGGAAATTTATTT
TAGATACTGATGCTAGTTTTGAAGCTATTGGAGCAGTTTAGAGCCAAATA
AAAGAAGATGGTTCAGATATAGTTATTGCATACAGTTAAAGACATCTGAC
CGCCATAACCGAAACTAGGAAAAAGTTGTTTGCGCTGCATGAATATGTTT
TATGTTTTAGACAGTATTTTTACGGAAAAGAATTTGTGACCCGAACAGAT
CATAAGGCTTTATGAACACGACAAAAAAATCAATAAGTCCACAATTTCAG
ACATGGGTGGCTAGTCTATCGGAATACAATTTTCAATTGAAATACCGGAA
AAGTGAAGAGCATGCAAATGCAGATGGATTATCAAAGATCGGGGTAGTTT
GTGCGCACAATGTCAGACAGCATATATACAACATAAACAAGAAAAACCAA
AAGTAAGATATATCAATTTGATTCAAAAAACACAGACAATGATAGAGATT
ATGGAGGAACAGAAGAAAGATAAATTGTTCAAGGAAGCAATGGAAATTCT
TAGGAATGAGATAGGCGTTTAAAATGAATCGTTTCAAAATAGTTTTTTAT
TTAAATATAAAGATCAGTTAAAGAAATCAGATGATATGCTTGTAATAGAA
ATGGATTAAATAGATCTAGTGGTAATCCCAAAAAGCTATCAATCTAAATT
AGTGATTAAAATACACGAAGATTTATGTCATATTGGAATTAAGAAACTGT
TTCACTACCTAGAAGGGAATTTCTTTTGGCAAAAATGTAAGAAACTGTTA
AAGATCTATTAAAACGATGTGTAACGTGTGCAAAAAGAAAAATAGACTAA
GGAAAGACAAAAGAAATTTTAATTCCACGCGAAAGTTCTGAATTTCTAGA
ACATATTTTTATGCATGTTGCTGTCATGGAGAGAACTTAAACCATAAAAA
AGTATGTTATTGTTATTATTGATAGGCTTAGCAAATTAGTAAGTCTAACG
GCTACATCAAACCAAGATGAAAAAACTATTTGGAAGACGCTAATGAAAAA
TTGGATTTACAAATACGGTAAACCTCAAACTATATTGACGGACAGAGGGC
GAAATTTTGAAAACAAATATTTAAAAGAAAAATTAGGACAGCTGGGAATA
AGACAAGAATTCGCTACGTCATATCAACATCAGTTGAATGGCTTAGTAGA
AAGAGCTATTATAACAATGAGAGATTTACTTGTGACAACGATGAAAGGAG
GCTGTGCTGAAAAACAGTGGCACGAACTTCTGCCTCGAATTGAATTCAGT
ATAAACGCAACTTATCAAAGTGCTACGAAATATTACCCATTTGAGATAGT
ATATGGTCGAAAAATAACATTAAATGCTAATTCTGGACTCCAAAATCAAG
TACAAGATATTTCAAAGAAAACTCAAATGAATTCCAAACAAGCTGTTAAA
AAGATGAGAGAATTCAAGGACGACAAAAGAATTAAATGAAATTTTGAAGT
GGGGGAGGAGATACTAGTATGCCAAGAACCACATAGAAGAAACAAATATA
TTATTCAATATGATGGTCCATATAAAATTCTCAGATTTATTTCAGAACAT
CTAATCGAATTGCAGTATCCAACGCAGCGCCAATGAATCGAATGTTTAAA
GCGATGGCATCAATCTTAAAAAGTGAAGAGTAATGATAGTACATTTAAGG
TCAAAGACCAAATGTTTAGCATTTTACTGTAAATTGCGTAACATAATACA
ATGTGTTAGTGATTGCGCAATATA
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protein sequence of SMED30031648-orf-1

>SMED30031648-orf-1 ID=SMED30031648-orf-1|Name=SMED30031648-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=149bp
MKNWIYKYGKPQTILTDRGRNFENKYLKEKLGQLGIRQEFATSYQHQLNG
LVERAIITMRDLLVTTMKGGCAEKQWHELLPRIEFSINATYQSATKYYPF
EIVYGRKITLNANSGLQNQVQDISKKTQMNSKQAVKKMREFKDDKRIK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR036397RNaseH_sf
IPR001584Integrase_cat-core
IPR012337RNaseH-like_sf
Vocabulary: biological process
TermDefinition
GO:0015074DNA integration
GO:0006508proteolysis
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
GO:0004190aspartic-type endopeptidase activity
GO:0008270zinc ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001584Integrase, catalytic corePFAMPF00665rvecoord: 3..61
e-value: 3.5E-8
score: 33.7
IPR001584Integrase, catalytic corePROSITEPS50994INTEGRASEcoord: 1..107
score: 15.597
IPR036397Ribonuclease H superfamilyGENE3DG3DSA:3.30.420.10coord: 1..145
e-value: 5.2E-32
score: 112.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 124..148
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 133..148
NoneNo IPR availablePANTHERPTHR24559:SF292coord: 2..139
NoneNo IPR availablePANTHERPTHR24559FAMILY NOT NAMEDcoord: 2..139
IPR012337Ribonuclease H-like superfamilySUPERFAMILYSSF53098Ribonuclease H-likecoord: 2..110