EMP24

Overview


Name	EMP24 (AHD24743.1)
Smed ID	SMED30031611
Length (bp)	1139

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of EMP24 (SMED30031611) t-SNE clustered cells

Violin plots show distribution of expression levels for EMP24 (SMED30031611) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of EMP24 (SMED30031611) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for EMP24 (SMED30031611) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30031611

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
epidermis	SMED30031611	SMESG000069470.1	dd_Smed_v4_2314_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30031611	SMESG000069470.1	dd_Smed_v4_2314_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30031611	SMESG000069470.1	dd_Smed_v4_2314_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Homology

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BLAST of EMP24 vs. Ensembl Human
Match: TMED2 (transmembrane p24 trafficking protein 2 [Source:HGNC Symbol;Acc:HGNC:16996])

HSP 1 Score: 254.988 bits (650), Expect = 7.852e-84
Identity = 108/169 (63.91%), Postives = 149/169 (88.17%), Query Frame = -1

Query:  432 GYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 938
            GY++++DA+++ECFF+R+ SG KM L+FEV EGGFLDIDV++ GPD K IY GDRES+GKY F+AH+DG YK+CFSN+MSTMTPKI+MF++D+ + P+  ++M T+AHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HR+IN+NTNSRVVLWS FEA++L++MT+GQ
Sbjct:   20 GYFVSIDAHAEECFFERVTSGTKMGLIFEVAEGGFLDIDVEITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPK-GQDMETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQ 187

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BLAST of EMP24 vs. Ensembl Human
Match: TMED2 (transmembrane p24 trafficking protein 2 [Source:HGNC Symbol;Acc:HGNC:16996])

HSP 1 Score: 204.912 bits (520), Expect = 1.068e-64
Identity = 89/148 (60.14%), Postives = 122/148 (82.43%), Query Frame = -1

Query:  516 GYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNT-------DAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHR 938
            GY++++DA+++ECFF+R+ SG KM L+FEV EGGFLDIDV++ GPD K IY GDRES+GKY F+AH+DG YK+CFSN+MSTMTPKI+MF++D+ + P+  ++M T       DAHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HR
Sbjct:   20 GYFVSIDAHAEECFFERVTSGTKMGLIFEVAEGGFLDIDVEITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPK-GQDMETEGGGDTWDAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHR 166

[Close]

BLAST of EMP24 vs. Ensembl Human
Match: TMED2 (transmembrane p24 trafficking protein 2 [Source:HGNC Symbol;Acc:HGNC:16996])

HSP 1 Score: 152.91 bits (385), Expect = 2.943e-45
Identity = 65/103 (63.11%), Postives = 92/103 (89.32%), Query Frame = -1

Query:  432 VDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 740
            +DG YK+CFSN+MSTMTPKI+MF++D+ + P+  ++M T+AHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HR+IN+NTNSRVVLWS FEA++L++MT+GQ
Sbjct:    1 MDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPK-GQDMETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQ 102

[Close]

BLAST of EMP24 vs. Ensembl Human
Match: TMED7 (transmembrane p24 trafficking protein 7 [Source:HGNC Symbol;Acc:HGNC:24253])

HSP 1 Score: 111.694 bits (278), Expect = 3.009e-28
Identity = 55/161 (34.16%), Postives = 89/161 (55.28%), Query Frame = -1

Query:  432 NSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 914
            N+ +CF++ +  G K  L F+V+ GG  D+D ++  PDGK +Y   ++    + F+A  +G YK+CFSN+ ST T K + F   V + P      N  +   ++E     +  +L +V   Q +  +RE   RS  E+ N+RV  WS  EA++LL ++IGQ
Sbjct:   44 NAKQCFYEDIAQGTKCTLEFQVITGGHYDVDCRLEDPDGKVLYKEMKKQYDSFTFTASKNGTYKFCFSNEFSTFTHKTVYFDFQVGEDPPLFPSENRVSALTQMESACVSIHEALKSVIDYQTHFRLREAQGRSRAEDLNTRVAYWSVGEALILLVVSIGQ 204

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BLAST of EMP24 vs. Ensembl Human
Match: TMED7-TICAM2 (TMED7-TICAM2 readthrough [Source:HGNC Symbol;Acc:HGNC:33945])

HSP 1 Score: 92.0485 bits (227), Expect = 2.159e-21
Identity = 46/144 (31.94%), Postives = 76/144 (52.78%), Query Frame = -1

Query:  483 NSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVV 914
            N+ +CF++ +  G K  L F+V+ GG  D+D ++  PDGK +Y   ++    + F+A  +G YK+CFSN+ ST T K + F   V + P      N  +   ++E     +  +L +V   Q +  +RE   RS  E+ N+RV 
Sbjct:   44 NAKQCFYEDIAQGTKCTLEFQVITGGHYDVDCRLEDPDGKVLYKEMKKQYDSFTFTASKNGTYKFCFSNEFSTFTHKTVYFDFQVGEDPPLFPSENRVSALTQMESACVSIHEALKSVIDYQTHFRLREAQGRSRAEDLNTRVA 187

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BLAST of EMP24 vs. Ensembl Celegans
Match: sel-9 (Suppressor/enhancer of lin-12 protein 9 [Source:UniProtKB/Swiss-Prot;Acc:O17528])

HSP 1 Score: 236.498 bits (602), Expect = 6.916e-77
Identity = 109/188 (57.98%), Postives = 143/188 (76.06%), Query Frame = -1

Query:  432 NISIFLILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVS---QKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 986
            N   +++    +     Y+I VDAN ++CFFDRL SG KM LMFEV EGGFLDIDVK+ GPD K IY G+RES+GK+ F+AH+DG+Y YCF NKMSTMTPK +MF+++++   Q+          A   KLE+MV ELS++L++VKH+QEY+EVRERVHR+INENTNSRVV+W+AFEA +L+ MT+GQ
Sbjct:    2 NSLTWILAVLFVTPAASYFIHVDANEEQCFFDRLTSGTKMGLMFEVAEGGFLDIDVKITGPDNKEIYKGERESSGKFTFAAHMDGVYTYCFGNKMSTMTPKAVMFTVEITEPHQQAPGAAANQDAADNAKLEEMVRELSSALMSVKHEQEYMEVRERVHRNINENTNSRVVMWAAFEAFVLVGMTVGQ 189

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BLAST of EMP24 vs. Ensembl Celegans
Match: F57B10.5 (pep chromosome:WBcel235:I:6562699:6563660:1 gene:WBGene00019003.1 transcript:F57B10.5.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F57B10.5)

HSP 1 Score: 90.8929 bits (224), Expect = 3.021e-21
Identity = 47/162 (29.01%), Postives = 83/162 (51.23%), Query Frame = -1

Query:  432 NSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQ-NKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 914
            N+++CF++ L      +  F+VV GG  D+D+ +  P+GK +Y   ++      F A V+G YK CFSN+ ST + KI+        +   +  +   AH   +LE+    + + L  +   Q +  +RE   R   E  N RV++WS  ++ +++ + IGQ
Sbjct:   27 NANQCFYEDLKKDVDTVFEFQVVTGGHYDVDLIIEDPNGKVLYKDTKKQYDSINFKAEVEGTYKACFSNEFSTFSHKIVYMDWQFGDQNALHAAVTQGAHAMTQLENYAVAIGDKLRTIDDYQTHHRLREATGRKRAEELNERVMIWSLGQSAVVVFIGIGQ 188

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BLAST of EMP24 vs. Ensembl Celegans
Match: Y60A3A.9 (pep chromosome:WBcel235:V:19946691:19949093:-1 gene:WBGene00013360.1 transcript:Y60A3A.9.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y60A3A.9)

HSP 1 Score: 50.447 bits (119), Expect = 4.410e-7
Identity = 41/191 (21.47%), Postives = 86/191 (45.03%), Query Frame = -1

Query:  453 ISIFLILCHLIFKIKGYYITVDANSDECFFDR-----LVSGAKMMLMFEVVEGGFLD-----IDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYC-FSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDM---VYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLL 983
            + + + L  L       Y  +     +CF +      +V+G   + +++    G+ D     + V+V  P+ K I +    + G++ F+++  G +  C +SN  +      +   +D+ Q     ++    A ++KL ++   + +L + +  +  +Q Y   RE   R  +E+TNSRV  WS  + V+L
Sbjct:    1 MRLLIALLSLATYADSLYFHIAETEKKCFIEEIPDETMVTGNYKVQLYDPNTKGYGDYPNIGMHVEVKDPEDKVILSKLYTAEGRFTFTSNTPGEHVICIYSNSTAWFNGAQLRVHLDI-QAGDHAQDYAQIAQKDKLNELQLRIRQLLDQVDQITKEQNYQRYREERFRQTSESTNSRVFYWSIAQVVVL 190

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BLAST of EMP24 vs. Ensembl Fly
Match: CHOp24 (gene:FBgn0029709 transcript:FBtr0343566)

HSP 1 Score: 195.282 bits (495), Expect = 9.715e-61
Identity = 85/171 (49.71%), Postives = 125/171 (73.10%), Query Frame = -1

Query:  432 GYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRE--NKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 938
             + ++VDA+++ECFF+ +  G K  + FEV++GGFLD+D+K+ GPD   ++  ++ES+GKY F A   G Y  CF+N+ S+MTPK++MFS+DV   P+         +    KLEDM+ ELS +L +VKH+QEY+ VR+++HRS+NENTNSRVVLWS FEA++L+ MT+GQ
Sbjct:   24 AFIVSVDAHNEECFFENVEGGTKFGVTFEVIDGGFLDVDIKISGPDNHVMHESEKESSGKYTFVAPAKGTYTVCFNNERSSMTPKLVMFSIDVGDAPQRAPGAPGEEEVGHTKLEDMIRELSGTLTSVKHEQEYMHVRDKIHRSVNENTNSRVVLWSTFEALVLVLMTVGQ 194

[Close]

BLAST of EMP24 vs. Ensembl Fly
Match: CHOp24 (gene:FBgn0029709 transcript:FBtr0070675)

HSP 1 Score: 195.282 bits (495), Expect = 9.715e-61
Identity = 85/171 (49.71%), Postives = 125/171 (73.10%), Query Frame = -1

Query:  432 GYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRE--NKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 938
             + ++VDA+++ECFF+ +  G K  + FEV++GGFLD+D+K+ GPD   ++  ++ES+GKY F A   G Y  CF+N+ S+MTPK++MFS+DV   P+         +    KLEDM+ ELS +L +VKH+QEY+ VR+++HRS+NENTNSRVVLWS FEA++L+ MT+GQ
Sbjct:   24 AFIVSVDAHNEECFFENVEGGTKFGVTFEVIDGGFLDVDIKISGPDNHVMHESEKESSGKYTFVAPAKGTYTVCFNNERSSMTPKLVMFSIDVGDAPQRAPGAPGEEEVGHTKLEDMIRELSGTLTSVKHEQEYMHVRDKIHRSVNENTNSRVVLWSTFEALVLVLMTVGQ 194

[Close]

BLAST of EMP24 vs. Ensembl Fly
Match: CG9308 (gene:FBgn0034681 transcript:FBtr0071749)

HSP 1 Score: 122.479 bits (306), Expect = 7.582e-33
Identity = 62/179 (34.64%), Postives = 106/179 (59.22%), Query Frame = -1

Query:  450 LILCHLIFKIK-GYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPR----ENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLL 971
            ++L  ++F+   G+ +T+DA+   CF+D      K+ + FEV+EGGF D+ V++ GPD   ++   +++ G + F+A  +G Y+ CF NKMSTMTPKI+MF   V++        +K ++    Q  ++ M+ +LS  L  VK +QEY+  R R H  +++    RV+ WS F  ++L+
Sbjct:    5 IVLVLVLFQAACGFIVTLDAHETMCFYDHANVSDKVTVSFEVMEGGFKDVGVEIAGPDDDRLHHSKQDTMGSFTFTAMKEGRYQLCFDNKMSTMTPKILMFQFHVARAIEFYMDSSKRVDDVIEQATVQSMINQLSAKLGAVKMEQEYMHFRYRGHLEVSDMVELRVLAWSIFGPMMLI 183

[Close]

BLAST of EMP24 vs. Ensembl Fly
Match: p24-1 (gene:FBgn0030341 transcript:FBtr0073604)

HSP 1 Score: 80.4925 bits (197), Expect = 2.807e-17
Identity = 47/182 (25.82%), Postives = 87/182 (47.80%), Query Frame = -1

Query:  432 ILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQ---NKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 968
            ++ H I  ++  +   D N+ +CF++ +   +     F+V  GG LD+DV +  P GK IY+ ++ +   + F A   G+Y  CF N+ S  + KI+     V ++P        D H     ++E     +   L ++   Q +  +RE   R   E+ N RV++WS+ E   ++ + + Q
Sbjct:   12 LMMHCISAVEFTFDLAD-NAVDCFYEEIKKNSSAYFEFQVSAGGQLDVDVTLKDPQGKVIYSLEKATFDSHQFVAETTGVYTACFGNQFSAFSHKIVYVDFQVGEEP---ALPGVDEHATVLTQMETSSQAIHKGLNDILDAQTHHRLREAQGRKRAEDLNQRVMVWSSLETAAVIVIGLVQ 189

[Close]

BLAST of EMP24 vs. Ensembl Fly
Match: p24-1 (gene:FBgn0030341 transcript:FBtr0334455)

HSP 1 Score: 80.4925 bits (197), Expect = 2.807e-17
Identity = 47/182 (25.82%), Postives = 87/182 (47.80%), Query Frame = -1

Query:  432 ILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQ---NKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 968
            ++ H I  ++  +   D N+ +CF++ +   +     F+V  GG LD+DV +  P GK IY+ ++ +   + F A   G+Y  CF N+ S  + KI+     V ++P        D H     ++E     +   L ++   Q +  +RE   R   E+ N RV++WS+ E   ++ + + Q
Sbjct:   12 LMMHCISAVEFTFDLAD-NAVDCFYEEIKKNSSAYFEFQVSAGGQLDVDVTLKDPQGKVIYSLEKATFDSHQFVAETTGVYTACFGNQFSAFSHKIVYVDFQVGEEP---ALPGVDEHATVLTQMETSSQAIHKGLNDILDAQTHHRLREAQGRKRAEDLNQRVMVWSSLETAAVIVIGLVQ 189

[Close]

BLAST of EMP24 vs. Ensembl Zebrafish
Match: tmed2 (transmembrane p24 trafficking protein 2 [Source:ZFIN;Acc:ZDB-GENE-030131-269])

HSP 1 Score: 263.462 bits (672), Expect = 2.349e-87
Identity = 112/182 (61.54%), Postives = 157/182 (86.26%), Query Frame = -1

Query:  432 IFLILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 977
            + ++LC L +   GY++++DA+++ECF++R+ SG KM LMFEV EGGFLDIDVK+ GPDGK IY GDRES+GKY+ +AH+DG YK+CFSN+MSTMTPKI+MF++D+ + P+  ++M T+AHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HR+IN+NTNSRVVLWS FEA++L++MT+GQ
Sbjct:    7 LIVLLCALSYTASGYFVSIDAHAEECFYERVNSGTKMSLMFEVAEGGFLDIDVKITGPDGKQIYKGDRESSGKYSVAAHMDGTYKFCFSNEMSTMTPKIVMFTIDIGEAPK-GQDMETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQ 187

[Close]

BLAST of EMP24 vs. Ensembl Zebrafish
Match: tmed2 (transmembrane p24 trafficking protein 2 [Source:ZFIN;Acc:ZDB-GENE-030131-269])

HSP 1 Score: 257.684 bits (657), Expect = 6.525e-85
Identity = 113/189 (59.79%), Postives = 157/189 (83.07%), Query Frame = -1

Query:  432 IFLILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNT-------DAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 977
            + ++LC L +   GY++++DA+++ECF++R+ SG KM LMFEV EGGFLDIDVK+ GPDGK IY GDRES+GKY+ +AH+DG YK+CFSN+MSTMTPKI+MF++D+ + P+  ++M T       DAHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HR+IN+NTNSRVVLWS FEA++L++MT+GQ
Sbjct:    7 LIVLLCALSYTASGYFVSIDAHAEECFYERVNSGTKMSLMFEVAEGGFLDIDVKITGPDGKQIYKGDRESSGKYSVAAHMDGTYKFCFSNEMSTMTPKIVMFTIDIGEAPK-GQDMETEGGGDSWDAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQ 194

[Close]

BLAST of EMP24 vs. Ensembl Zebrafish
Match: tmed7 (transmembrane p24 trafficking protein 7 [Source:ZFIN;Acc:ZDB-GENE-040426-2570])

HSP 1 Score: 105.531 bits (262), Expect = 2.782e-26
Identity = 54/161 (33.54%), Postives = 85/161 (52.80%), Query Frame = -1

Query:  432 NSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 914
            N+ +CF++ +  G K  L F+VV GG  D+D ++  P+G  +Y   ++    + FSA  +G YK+CFSN+ ST T K + F   V   P      N      ++E     +  +L +V   Q +  +RE   RS  E+ NSRV  WS  EA++LL +++ Q
Sbjct:   36 NAKQCFYEDITIGTKCTLEFQVVTGGHYDVDCRLEDPEGTVLYKEMKKQYDSFTFSAARNGTYKFCFSNEFSTFTHKTVYFDFQVGDDPPLFPNENRVTALTQMESACVSIHEALKSVMDYQTHFRLREAQGRSRAEDLNSRVAFWSVGEALILLVVSVSQ 196

[Close]

Query:  432 NSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 914
            N+ +CF++ +  G K  L F+VV GG  D+D ++  P+G  +Y   ++    + FSA  +G YK+CFSN+ ST T K + F   V   P      N      ++E     +  +L +V   Q +  +RE   RS  E+ NSRV  WS  EA++LL +++ Q
Sbjct:   36 NAKQCFYEDITIGTKCTLEFQVVTGGHYDVDCRLEDPEGTVLYKEMKKQYDSFTFSAARNGTYKFCFSNEFSTFTHKTVYFDFQVGDDPPLFPNENRVTALTQMESACVSIHEALKSVMDYQTHFRLREAQGRSRAEDLNSRVAFWSVGEALILLVVSVSQ 196

[Close]

Query:  432 NSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 914
            N+ +CF++ +  G K  L F+VV GG  D+D ++  P+G  +Y   ++    + FSA  +G YK+CFSN+ ST T K + F   V   P      N      ++E     +  +L +V   Q +  +RE   RS  E+ NSRV  WS  EA++LL +++ Q
Sbjct:   36 NAKQCFYEDITIGTKCTLEFQVVTGGHYDVDCRLEDPEGTVLYKEMKKQYDSFTFSAARNGTYKFCFSNEFSTFTHKTVYFDFQVGDDPPLFPNENRVTALTQMESACVSIHEALKSVMDYQTHFRLREAQGRSRAEDLNSRVAFWSVGEALILLVVSVSQ 196

[Close]

BLAST of EMP24 vs. Ensembl Xenopus
Match: tmed2 (transmembrane p24 trafficking protein 2 [Source:NCBI gene;Acc:734103])

HSP 1 Score: 254.988 bits (650), Expect = 8.562e-84
Identity = 108/179 (60.34%), Postives = 152/179 (84.92%), Query Frame = -1

Query:  432 ILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 968
            +LC       GY++++DA+++ECFF+++ SG KM L+FEV EGGFLDIDV++ GPD K I+ GDRES+GKY F+AH+DG YK+CFSN+MSTMTPKI+MF++D+ + P+  ++M T+AHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HR+IN+NTNSRVVLWS FEA++L++MT+GQ
Sbjct:   10 LLCFFSATASGYFVSIDAHAEECFFEQVTSGTKMGLIFEVAEGGFLDIDVEITGPDNKGIHKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPK-GQDMETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQ 187

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BLAST of EMP24 vs. Ensembl Xenopus
Match: tmed2 (transmembrane p24 trafficking protein 2 [Source:NCBI gene;Acc:734103])

HSP 1 Score: 249.21 bits (635), Expect = 1.670e-81
Identity = 109/186 (58.60%), Postives = 152/186 (81.72%), Query Frame = -1

Query:  432 ILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNT-------DAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 968
            +LC       GY++++DA+++ECFF+++ SG KM L+FEV EGGFLDIDV++ GPD K I+ GDRES+GKY F+AH+DG YK+CFSN+MSTMTPKI+MF++D+ + P+  ++M T       DAHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HR+IN+NTNSRVVLWS FEA++L++MT+GQ
Sbjct:   10 LLCFFSATASGYFVSIDAHAEECFFEQVTSGTKMGLIFEVAEGGFLDIDVEITGPDNKGIHKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPK-GQDMETEGIGESWDAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQ 194

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BLAST of EMP24 vs. Ensembl Xenopus
Match: serinc2 (serine incorporator 2 [Source:Xenbase;Acc:XB-GENE-968901])

HSP 1 Score: 110.153 bits (274), Expect = 9.795e-28
Identity = 55/161 (34.16%), Postives = 86/161 (53.42%), Query Frame = -1

Query:  432 NSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 914
            N+ +CF++ +  G K  L F+V+ GG  D+D ++  PDG  +Y   ++    + F+A  +G YK+CFSN+ ST T K + F   V   P      N      ++E     +  +L +V   Q +  +RE   RS  E+ NSRV  WS  EA++LL ++IGQ
Sbjct:   45 NAKQCFYEDITQGTKCTLEFQVITGGHYDVDCRLEDPDGIVLYKEMKKQYDSFTFTATRNGTYKFCFSNEFSTFTHKTVYFDFQVGDDPPLFPNENRATALTQMESSCVSIHEALKSVIDYQTHFRLREAQGRSRAEDLNSRVAYWSIGEAIILLVVSIGQ 205

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BLAST of EMP24 vs. Ensembl Xenopus
Match: serinc2 (serine incorporator 2 [Source:Xenbase;Acc:XB-GENE-968901])

HSP 1 Score: 109.383 bits (272), Expect = 1.740e-27
Identity = 55/161 (34.16%), Postives = 86/161 (53.42%), Query Frame = -1

Query:  432 NSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 914
            N+ +CF++ +  G K  L F+V+ GG  D+D ++  PDG  +Y   ++    + F+A  +G YK+CFSN+ ST T K + F   V   P      N      ++E     +  +L +V   Q +  +RE   RS  E+ NSRV  WS  EA++LL ++IGQ
Sbjct:   39 NAKQCFYEDITQGTKCTLEFQVITGGHYDVDCRLEDPDGIVLYKEMKKQYDSFTFTATRNGTYKFCFSNEFSTFTHKTVYFDFQVGDDPPLFPNENRATALTQMESSCVSIHEALKSVIDYQTHFRLREAQGRSRAEDLNSRVAYWSIGEAIILLVVSIGQ 199

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BLAST of EMP24 vs. Ensembl Xenopus
Match: tmed5 (transmembrane p24 trafficking protein 5 [Source:NCBI gene;Acc:549314])

HSP 1 Score: 82.8037 bits (203), Expect = 9.275e-18
Identity = 53/202 (26.24%), Postives = 97/202 (48.02%), Query Frame = -1

Query:  432 IFLILCHLIFK---------IKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEM--------NTDAHQNKLEDMVYELSN---SLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 977
            +FL+L H   +            Y  T+ A   ECFF  +   A + + ++V++G  LD+D  +  P+G+ + + +R+S+G ++    +DG Y++CF N  S M+ K+I F + +     E  E+         TD    KLED++  +++    L      Q  L+  E   R++ E+   RV  WS F   +++ ++  Q
Sbjct:    7 LFLMLTHYTVRHCMGFSQSVDSDYTFTLPAGQKECFFQPMKRDATLEIEYQVLDGAGLDVDFSLTSPNGELLLSEERQSDGVHSVET-IDGDYQFCFDNSFSRMSEKVIFFELILDHLNDEGNELEDWKSYITGTDLLDMKLEDILETINSVKGRLTKSAQIQTLLKAFEARDRNLQESNFERVTFWSVFNLTVMVVVSALQ 207

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BLAST of EMP24 vs. Ensembl Mouse
Match: Tmed2 (transmembrane p24 trafficking protein 2 [Source:MGI Symbol;Acc:MGI:1929269])

HSP 1 Score: 254.988 bits (650), Expect = 6.208e-84
Identity = 108/169 (63.91%), Postives = 149/169 (88.17%), Query Frame = -1

Query:  432 GYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 938
            GY++++DA+++ECFF+R+ SG KM L+FEV EGGFLDIDV++ GPD K IY GDRES+GKY F+AH+DG YK+CFSN+MSTMTPKI+MF++D+ + P+  ++M T+AHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HR+IN+NTNSRVVLWS FEA++L++MT+GQ
Sbjct:   20 GYFVSIDAHAEECFFERVTSGTKMGLIFEVAEGGFLDIDVEITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPK-GQDMETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQ 187

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BLAST of EMP24 vs. Ensembl Mouse
Match: Tmed2 (transmembrane p24 trafficking protein 2 [Source:MGI Symbol;Acc:MGI:1929269])

HSP 1 Score: 193.356 bits (490), Expect = 3.419e-60
Identity = 82/129 (63.57%), Postives = 113/129 (87.60%), Query Frame = -1

Query:  432 KVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 818
            ++ GPD K IY GDRES+GKY F+AH+DG YK+CFSN+MSTMTPKI+MF++D+ + P+  ++M T+AHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HR+IN+NTNSRVVLWS FEA++L++MT+GQ
Sbjct:   35 RITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPK-GQDMETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQ 162

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BLAST of EMP24 vs. Ensembl Mouse
Match: Tmed2 (transmembrane p24 trafficking protein 2 [Source:MGI Symbol;Acc:MGI:1929269])

HSP 1 Score: 127.102 bits (318), Expect = 1.681e-35
Identity = 58/99 (58.59%), Postives = 82/99 (82.83%), Query Frame = -1

Query:  432 KMSTMTPKIIMFSMDVSQKPRENKEMNT-------DAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 707
            +MSTMTPKI+MF++D+ + P+  ++M T       DAHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HR+IN+NTNSRVVLWS FEA++L++MT+GQ
Sbjct:    1 RMSTMTPKIVMFTIDIGEAPK-GQDMETEGGGDSWDAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQ 98

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BLAST of EMP24 vs. Ensembl Mouse
Match: Tmed7 (transmembrane p24 trafficking protein 7 [Source:MGI Symbol;Acc:MGI:1913926])

HSP 1 Score: 111.309 bits (277), Expect = 3.282e-28
Identity = 54/161 (33.54%), Postives = 89/161 (55.28%), Query Frame = -1

Query:  432 NSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 914
            N+ +CF++ +  G K  L F+V+ GG  D+D ++  PDGK +Y   ++    + F+A  +G YK+CFSN+ ST T K + F   V + P      N  +   ++E     +  +L +V   Q +  +RE   RS  E+ N+RV  WS  EA++LL +++GQ
Sbjct:   44 NAKQCFYEDITQGTKCTLEFQVITGGHYDVDCRLEDPDGKVLYKEMKKQYDSFTFTASRNGTYKFCFSNEFSTFTHKTVYFDFQVGEDPPLFPSENRVSALTQMESACVSIHEALKSVIDYQTHFRLREAQGRSRAEDLNTRVAYWSVGEALILLVVSVGQ 204

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BLAST of EMP24 vs. Ensembl Mouse
Match: Tmed7 (transmembrane p24 trafficking protein 7 [Source:MGI Symbol;Acc:MGI:1913926])

HSP 1 Score: 92.0485 bits (227), Expect = 1.467e-21
Identity = 46/144 (31.94%), Postives = 76/144 (52.78%), Query Frame = -1

Query:  483 NSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVV 914
            N+ +CF++ +  G K  L F+V+ GG  D+D ++  PDGK +Y   ++    + F+A  +G YK+CFSN+ ST T K + F   V + P      N  +   ++E     +  +L +V   Q +  +RE   RS  E+ N+RV 
Sbjct:   44 NAKQCFYEDITQGTKCTLEFQVITGGHYDVDCRLEDPDGKVLYKEMKKQYDSFTFTASRNGTYKFCFSNEFSTFTHKTVYFDFQVGEDPPLFPSENRVSALTQMESACVSIHEALKSVIDYQTHFRLREAQGRSRAEDLNTRVA 187

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BLAST of EMP24 vs. UniProt/SwissProt
Match: sp|P49020|TMED2_CRIGR (Transmembrane emp24 domain-containing protein 2 (Fragment) OS=Cricetulus griseus OX=10029 GN=TMED2 PE=1 SV=1)

HSP 1 Score: 254.988 bits (650), Expect = 3.402e-83
Identity = 108/169 (63.91%), Postives = 149/169 (88.17%), Query Frame = -1

Query:  432 GYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 938
            GY++++DA+++ECFF+R+ SG KM L+FEV EGGFLDIDV++ GPD K IY GDRES+GKY F+AH+DG YK+CFSN+MSTMTPKI+MF++D+ + P+  ++M T+AHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HR+IN+NTNSRVVLWS FEA++L++MT+GQ
Sbjct:   15 GYFVSIDAHAEECFFERVTSGTKMGLIFEVAEGGFLDIDVEITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPK-GQDMETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQ 182

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BLAST of EMP24 vs. UniProt/SwissProt
Match: sp|Q63524|TMED2_RAT (Transmembrane emp24 domain-containing protein 2 OS=Rattus norvegicus OX=10116 GN=Tmed2 PE=1 SV=1)

HSP 1 Score: 254.988 bits (650), Expect = 3.771e-83
Identity = 108/169 (63.91%), Postives = 149/169 (88.17%), Query Frame = -1

Query:  432 GYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 938
            GY++++DA+++ECFF+R+ SG KM L+FEV EGGFLDIDV++ GPD K IY GDRES+GKY F+AH+DG YK+CFSN+MSTMTPKI+MF++D+ + P+  ++M T+AHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HR+IN+NTNSRVVLWS FEA++L++MT+GQ
Sbjct:   20 GYFVSIDAHAEECFFERVTSGTKMGLIFEVAEGGFLDIDVEITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPK-GQDMETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQ 187

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BLAST of EMP24 vs. UniProt/SwissProt
Match: sp|Q15363|TMED2_HUMAN (Transmembrane emp24 domain-containing protein 2 OS=Homo sapiens OX=9606 GN=TMED2 PE=1 SV=1)

HSP 1 Score: 254.988 bits (650), Expect = 3.771e-83
Identity = 108/169 (63.91%), Postives = 149/169 (88.17%), Query Frame = -1

Query:  432 GYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 938
            GY++++DA+++ECFF+R+ SG KM L+FEV EGGFLDIDV++ GPD K IY GDRES+GKY F+AH+DG YK+CFSN+MSTMTPKI+MF++D+ + P+  ++M T+AHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HR+IN+NTNSRVVLWS FEA++L++MT+GQ
Sbjct:   20 GYFVSIDAHAEECFFERVTSGTKMGLIFEVAEGGFLDIDVEITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPK-GQDMETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQ 187

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BLAST of EMP24 vs. UniProt/SwissProt
Match: sp|Q9R0Q3|TMED2_MOUSE (Transmembrane emp24 domain-containing protein 2 OS=Mus musculus OX=10090 GN=Tmed2 PE=1 SV=1)

HSP 1 Score: 254.988 bits (650), Expect = 4.343e-83
Identity = 108/169 (63.91%), Postives = 149/169 (88.17%), Query Frame = -1

Query:  432 GYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 938
            GY++++DA+++ECFF+R+ SG KM L+FEV EGGFLDIDV++ GPD K IY GDRES+GKY F+AH+DG YK+CFSN+MSTMTPKI+MF++D+ + P+  ++M T+AHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HR+IN+NTNSRVVLWS FEA++L++MT+GQ
Sbjct:   20 GYFVSIDAHAEECFFERVTSGTKMGLIFEVAEGGFLDIDVEITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPK-GQDMETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQ 187

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BLAST of EMP24 vs. UniProt/SwissProt
Match: sp|O17528|TMED2_CAEEL (Suppressor/enhancer of lin-12 protein 9 OS=Caenorhabditis elegans OX=6239 GN=sel-9 PE=1 SV=1)

HSP 1 Score: 236.498 bits (602), Expect = 8.797e-76
Identity = 109/188 (57.98%), Postives = 143/188 (76.06%), Query Frame = -1

Query:  432 NISIFLILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVS---QKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 986
            N   +++    +     Y+I VDAN ++CFFDRL SG KM LMFEV EGGFLDIDVK+ GPD K IY G+RES+GK+ F+AH+DG+Y YCF NKMSTMTPK +MF+++++   Q+          A   KLE+MV ELS++L++VKH+QEY+EVRERVHR+INENTNSRVV+W+AFEA +L+ MT+GQ
Sbjct:    2 NSLTWILAVLFVTPAASYFIHVDANEEQCFFDRLTSGTKMGLMFEVAEGGFLDIDVKITGPDNKEIYKGERESSGKFTFAAHMDGVYTYCFGNKMSTMTPKAVMFTVEITEPHQQAPGAAANQDAADNAKLEEMVRELSSALMSVKHEQEYMEVRERVHRNINENTNSRVVMWAAFEAFVLVGMTVGQ 189

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BLAST of EMP24 vs. TrEMBL
Match: V9XRM7 (EMP24 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 389.808 bits (1000), Expect = 9.290e-134
Identity = 187/187 (100.00%), Postives = 187/187 (100.00%), Query Frame = -1

Query:  432 MRNISIFLILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 992
            MRNISIFLILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ
Sbjct:    1 MRNISIFLILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 187

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BLAST of EMP24 vs. TrEMBL
Match: A0A1I8HUD8 (GOLD domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig002731g1 PE=3 SV=1)

HSP 1 Score: 278.87 bits (712), Expect = 5.022e-90
Identity = 121/179 (67.60%), Postives = 154/179 (86.03%), Query Frame = -1

Query:  432 ILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 968
            +   L      Y+IT+DAN ++CFFDR+VSG KM LMFEV EGGFLDIDVK++GPD K +Y+GDRESNGKY F+AH+DGIYKYCFSNKMSTMTPKI++FSMD+ +KP+++K+M+ D H NKLE+M+ +LS SL  VKH+QEY+EVRER+HRSIN+NTNSRVVLWS FEA++L++MT+GQ
Sbjct:    8 LFSALFVSCNAYFITIDANEEQCFFDRVVSGTKMTLMFEVAEGGFLDIDVKLYGPDNKVVYSGDRESNGKYTFAAHMDGIYKYCFSNKMSTMTPKIVLFSMDIGEKPKDSKDMSADEHHNKLEEMINQLSTSLTVVKHEQEYMEVRERIHRSINDNTNSRVVLWSFFEALVLIAMTVGQ 186

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BLAST of EMP24 vs. TrEMBL
Match: C3XVK2 (GOLD domain-containing protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_127851 PE=3 SV=1)

HSP 1 Score: 271.937 bits (694), Expect = 2.902e-87
Identity = 118/188 (62.77%), Postives = 158/188 (84.04%), Query Frame = -1

Query:  432 NMRNISIFLILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 995
             +RN+ +  +L   +  I  Y+++VDA+++ECFFD++ SG KM LMFEV EGGFLDIDVK+ GPD   IY+G+RESNGKY F+AH+DG+YKYCFSNKMSTMTPKI+MFSMD+ + P+  ++M T+AHQNKLE+M+ EL+ +L  VKH+QEY+EVRER+HR+IN+NTNSRVVLWS FEA++L++MT+GQ
Sbjct:    3 TLRNLWLLAVLAACLDSISAYFVSVDAHAEECFFDKVTSGTKMGLMFEVAEGGFLDIDVKITGPDSTVIYSGERESNGKYTFAAHMDGVYKYCFSNKMSTMTPKIVMFSMDIGEAPK-GQDMETEAHQNKLEEMINELAVALTGVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQ 189

[Close]

BLAST of EMP24 vs. TrEMBL
Match: A0A2C9JWM8 (GOLD domain-containing protein OS=Biomphalaria glabrata OX=6526 GN=106070911 PE=3 SV=1)

HSP 1 Score: 267.314 bits (682), Expect = 2.111e-85
Identity = 119/186 (63.98%), Postives = 157/186 (84.41%), Query Frame = -1

Query:  432 NISIFLI-LCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 986
            NIS F++ L  LI   + Y+I++DA+++ECFFD++ SG KM LMFEV EGGFLDID+K++GPDGK I++GDRESNGKY F+AH+DG YKYCFSN MSTMTPKI++FS+D+ +KP++      DA+QNKL +M+ ELS +L  VKH+QEY+EVRER+HRSIN+NTNSRVVLWS FEA++L++MT+GQ
Sbjct:    5 NISTFIVTLLSLIQSSEQYFISIDAHAEECFFDKVTSGTKMSLMFEVAEGGFLDIDIKIYGPDGKVIHSGDRESNGKYTFAAHMDGNYKYCFSNAMSTMTPKIVVFSIDIGEKPKDAAGEEGDANQNKLTEMINELSTALTGVKHEQEYMEVRERIHRSINDNTNSRVVLWSFFEALVLVAMTLGQ 190

[Close]

BLAST of EMP24 vs. TrEMBL
Match: X1ZJR4 (GOLD domain-containing protein OS=Capitella teleta OX=283909 PE=3 SV=1)

HSP 1 Score: 266.159 bits (679), Expect = 5.874e-85
Identity = 119/182 (65.38%), Postives = 154/182 (84.62%), Query Frame = -1

Query:  432 LILCHLIFKIK-GYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTD-AHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 971
            ++ C ++FK    Y+I +DAN++ECFFD++ SG KM LMFEV EGGFLDIDVK+ GPDGK IY G+RESNGKY F+AH+DG+YKYCFSN+MSTMTPKI+MFSMD+  KP+ + +  ++ AH NKLE+M+ ELS SL  VKH+QEY+EVRER+HR+IN+NTNSRVVLWS FEA++L++MT+GQ
Sbjct:   10 VLACLMLFKASDAYFINIDANAEECFFDKVTSGTKMSLMFEVAEGGFLDIDVKIVGPDGKNIYYGERESNGKYTFAAHMDGVYKYCFSNQMSTMTPKIVMFSMDIGDKPKVDMDKESEAAHHNKLEEMINELSTSLTGVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLIAMTLGQ 191

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BLAST of EMP24 vs. Ensembl Cavefish
Match: tmed2 (transmembrane emp24 domain-containing protein 2 [Source:NCBI gene;Acc:111194998])

HSP 1 Score: 255.373 bits (651), Expect = 4.057e-84
Identity = 110/169 (65.09%), Postives = 150/169 (88.76%), Query Frame = -1

Query:  432 GYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 938
            GY+++VDA+++ECF++R+ SG KM LMFEV EGGFLDIDV++ GPDGK IY GDRES+GKY+ +AH+DG YK+CFSNKMSTMTPKI+MF++D+ + P+  ++M T+AHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HR+IN+NTNSRVVLWS FEA++L++MT+GQ
Sbjct:   21 GYFVSVDAHAEECFYERVNSGTKMGLMFEVAEGGFLDIDVEITGPDGKQIYKGDRESSGKYSVAAHMDGTYKFCFSNKMSTMTPKIVMFTIDIGEAPK-GQDMETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQ 188

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BLAST of EMP24 vs. Ensembl Cavefish
Match: TMED2 (transmembrane emp24 domain-containing protein 2 [Source:NCBI gene;Acc:103022411])

HSP 1 Score: 251.521 bits (641), Expect = 1.552e-82
Identity = 112/189 (59.26%), Postives = 154/189 (81.48%), Query Frame = -1

Query:  432 IFLILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNT-------DAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 977
            + +++  L     GY+++VDA+++ECF++R+ SG KM LMFEV EGGFLDIDV++ GPDGK IY GDRES+GKY+ +AH+DG YK+CFSNKMSTMTPKI+MF++D+ + P+ +  M T       DAHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HR+IN+NTNSRVVLWS FEA++L++MT+GQ
Sbjct:    7 LMVLMAALSATASGYFVSVDAHAEECFYERVNSGTKMGLMFEVAEGGFLDIDVEITGPDGKQIYKGDRESSGKYSVAAHMDGTYKFCFSNKMSTMTPKIVMFTIDIGEAPKGDG-METEVGGGTWDAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQ 194

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Query:  432 IFLILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNT-------DAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 977
            + +++  L     GY+++VDA+++ECF++R+ SG KM LMFEV EGGFLDIDV++ GPDGK IY GDRES+GKY+ +AH+DG YK+CFSNKMSTMTPKI+MF++D+ + P+ +  M T       DAHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HR+IN+NTNSRVVLWS FEA++L++MT+GQ
Sbjct:    7 LMVLMAALSATASGYFVSVDAHAEECFYERVNSGTKMGLMFEVAEGGFLDIDVEITGPDGKQIYKGDRESSGKYSVAAHMDGTYKFCFSNKMSTMTPKIVMFTIDIGEAPKGDG-METEVGGGTWDAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQ 194

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BLAST of EMP24 vs. Ensembl Cavefish
Match: TMED2 (transmembrane emp24 domain-containing protein 2 [Source:NCBI gene;Acc:103022411])

HSP 1 Score: 249.595 bits (636), Expect = 7.105e-82
Identity = 112/190 (58.95%), Postives = 154/190 (81.05%), Query Frame = -1

Query:  432 IFLILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPR----ENKEMNTD----AHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 977
            + +++  L     GY+++VDA+++ECF++R+ SG KM LMFEV EGGFLDIDV++ GPDGK IY GDRES+GKY+ +AH+DG YK+CFSNKMSTMTPKI+MF++D+ + P+    E + M  D    AHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HR+IN+NTNSRVVLWS FEA++L++MT+GQ
Sbjct:    7 LMVLMAALSATASGYFVSVDAHAEECFYERVNSGTKMGLMFEVAEGGFLDIDVEITGPDGKQIYKGDRESSGKYSVAAHMDGTYKFCFSNKMSTMTPKIVMFTIDIGEAPKGDGMETEGMIGDCSVTAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQ 196

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BLAST of EMP24 vs. Ensembl Cavefish
Match: tmed7 (transmembrane p24 trafficking protein 7 [Source:ZFIN;Acc:ZDB-GENE-040426-2570])

HSP 1 Score: 108.227 bits (269), Expect = 2.928e-27
Identity = 59/190 (31.05%), Postives = 98/190 (51.58%), Query Frame = -1

Query:  432 RGNMRNISIFLILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 1001
            RG    + + ++LC  ++  +  +   D N+ +CF++ +  G K  L F+VV GG  D+D ++  PDG  +Y   ++    + F+A  +G YK+CFSN+ ST T K + F   V + P      N      ++E     +  +L +V   Q +  +RE   RS  E+ N+RV  WS  EA +LL ++I Q
Sbjct:   12 RGWTGRLLLQVLLCGWVWGSELTFELPD-NAKQCFYEDITIGTKCTLEFQVVTGGHYDVDCRLEDPDGTVLYKEMKKQYDSFTFTAAKNGTYKFCFSNEFSTFTHKTVYFDFQVGEDPPLFPNENRVTALTQMESACVSIHEALKSVIDYQTHFRLREAQGRSRAEDLNTRVAFWSIGEAFILLVVSISQ 200

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BLAST of EMP24 vs. Ensembl Sea Lamprey
Match: tmed2 (transmembrane p24 trafficking protein 2 [Source:ZFIN;Acc:ZDB-GENE-030131-269])

HSP 1 Score: 197.593 bits (501), Expect = 4.195e-63
Identity = 82/130 (63.08%), Postives = 115/130 (88.46%), Query Frame = -1

Query:  432 VKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 821
            V++ GPDG+ IY GDRESNGKY F+AH+DG+YK+CF NKMSTMTPK++MF++D+ + P+  ++M T+AHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HRS+N+NTNSRVVLW+ FEA++L++MT+GQ
Sbjct:    1 VQITGPDGREIYRGDRESNGKYTFAAHMDGVYKFCFGNKMSTMTPKMVMFTIDMGETPK-GQDMETEAHQNKLEEMINELATAMTAVKHEQEYMEVRERIHRSVNDNTNSRVVLWAFFEALVLVAMTLGQ 129

[Close]

BLAST of EMP24 vs. Ensembl Sea Lamprey
Match: tmed7 (transmembrane p24 trafficking protein 7 [Source:ZFIN;Acc:ZDB-GENE-040426-2570])

HSP 1 Score: 90.5077 bits (223), Expect = 1.845e-21
Identity = 50/168 (29.76%), Postives = 82/168 (48.81%), Query Frame = -1

Query:  432 YITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVS-QKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 932
            ++ + A    C F  +  G      ++V+ GG  D+D +V GP G+ +Y   R+    + ++A   GIY  CFSN+ ST + K + F   +  +KP      N      ++E     +  +L +V   Q +  + E   R   E+ NSRV LWS  E ++LLS+ +GQ
Sbjct:   31 HLGLPAQQCRCLFMPIKVGRTCSKEWQVITGGNYDVDCRVEGPSGEILYKEMRKQYDSHVWTAEKKGIYAICFSNEFSTFSHKTVYFDWQIGDEKPVGKASTNGAVSLTQMESACVTIHEALKSVISYQTHHRLEEAQDRYQAEHLNSRVTLWSVGETLMLLSVVVGQ 198

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BLAST of EMP24 vs. Ensembl Sea Lamprey
Match: tmed3 (transmembrane p24 trafficking protein 3 [Source:ZFIN;Acc:ZDB-GENE-030131-8335])

HSP 1 Score: 90.5077 bits (223), Expect = 2.248e-21
Identity = 50/162 (30.86%), Postives = 84/162 (51.85%), Query Frame = -1

Query:  432 NSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQK-PRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 914
            N+ +CF++ +  G K+ L F+V+ GG  D+D +V GP    +Y  +R+    YA++    G Y+ CFSN+ ST T K + F + V ++ P    E        ++E     +   L +V + Q    ++E   R   E  NSRV +WS  E ++L+ + +GQ
Sbjct:   52 NAKQCFYEDIDQGVKVTLDFQVITGGNYDVDCRVKGPHDSVLYNENRKQYDSYAWTTEQRGTYEICFSNEFSTFTHKTVYFDLQVGEEAPLFRDEGTRVVALTQMESACVTIHEGLKSVDNYQTQHRLQETQDRHQAEVLNSRVNMWSIGETIILVVVGVGQ 213

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BLAST of EMP24 vs. Ensembl Sea Lamprey
Match: tmed5 (transmembrane p24 trafficking protein 5 [Source:ZFIN;Acc:ZDB-GENE-040426-1302])

HSP 1 Score: 76.6406 bits (187), Expect = 1.543e-16
Identity = 48/173 (27.75%), Postives = 85/173 (49.13%), Query Frame = -1

Query:  450 YYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFS--MDVSQKPRENKEMNTD-AHQNKLEDMVYE-LSNSLLNVK-------HQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLL 935
            +  ++ A   ECF+  L +GA M + ++V++G  +D+D  +  P G+ ++   R+S G  +  A  DG Y +CF N  S ++ K++ F   +D  +  +++K    D    +++ DM  E L  ++ N+K         Q  L V E   R + E     V LWS    V+L+
Sbjct:   30 FTFSLPAGRTECFYQALTAGASMEVEYQVIDGAGMDVDFSISSPTGQVLFAEYRKSEGLQSVEAKQDGDYMFCFDNSFSHVSEKVVFFEVILDNMEVEKDDKPAWKDFVEVDEIIDMKLEDLQETMDNIKTRLGKSIQMQSVLRVYEARDRKLQETNFEMVNLWSGISMVVLV 202

[Close]

BLAST of EMP24 vs. Ensembl Sea Lamprey
Match: tmed5 (transmembrane p24 trafficking protein 5 [Source:ZFIN;Acc:ZDB-GENE-040426-1302])

HSP 1 Score: 75.485 bits (184), Expect = 4.527e-16
Identity = 49/168 (29.17%), Postives = 85/168 (50.60%), Query Frame = -1

Query:  432 ECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQ-KPRENKEMNTDAHQN------KLEDMVYELSN--SLLNVKHQ-QEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 905
            ECF+  +  G  + + ++V++GG LD+D  V  P G  +    R+ +G ++     +G Y+ CF N  S ++ K+I F + +   +P E +    DA         KLED+   L++  + L+  HQ Q  L   E   R++ E+  +RV LWSA   + L+ ++  Q
Sbjct:   49 ECFYQPVKPGTVVEIEYQVIDGGELDVDFTVTAPSGTVLLAEHRQGDGVHSVDVKEEGDYEICFDNSFSHISEKLIFFEILLDHMQPEEERPEWMDAISADHLIGMKLEDLQESLNSVKARLSRSHQIQTVLRAFEARDRNLQESNLARVNLWSAASVLALMGVSALQ 216

[Close]

BLAST of EMP24 vs. Ensembl Yeast
Match: EMP24 (Component of the p24 complex; role in misfolded protein quality control; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles [Source:SGD;Acc:S000003168])

HSP 1 Score: 80.8777 bits (198), Expect = 1.914e-18
Identity = 53/193 (27.46%), Postives = 94/193 (48.70%), Query Frame = -1

Query:  432 FLILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVE-----GGFLDIDVKVFGPDGKAIYTGDRE-SNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSM------DVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 974
            F+I C L F    + + + A    CFF+ L  G ++ + F+  +        L  D  ++GP+   +    R+ S+G+   SA   G ++YCF N+ + +  K + F++      D+            D + N L+  V +LS     VK +Q Y+ +RER HR+  E+TN RV  WS F+  ++++ ++ Q
Sbjct:    8 FVIACFLFFSASAHNVLLPAYGRRCFFEDLSKGDELSISFQFGDRNPQSSSQLTGDFIIYGPERHEVLKTVRDTSHGEITLSAPYKGHFQYCFLNENTGIETKDVTFNIHGVVYVDLD-----------DPNTNTLDSAVRKLSKLTREVKDEQSYIVIRERTHRNTAESTNDRVKWWSIFQLGVVIANSLFQ 189

[Close]

BLAST of EMP24 vs. Ensembl Yeast
Match: ERP4 (Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; ERP4 has a paralog, ERP2, that arose from the whole genome duplication [Source:SGD;Acc:S000005542])

HSP 1 Score: 55.0694 bits (131), Expect = 2.402e-9
Identity = 39/167 (23.35%), Postives = 80/167 (47.90%), Query Frame = -1

Query:  432 ITVDANSDECFFDRLVSGAKMMLM-FEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 929
            I++ A + EC +  L S   ++++ ++V+ GG  +ID  +  PDG  I T  ++ +  +   +   G Y +C SN   T +PK +  +++  ++   + E   D   N   + + E+  +L  +    +YL  RE  +     +T SR+   S     +++ ++I Q
Sbjct:   29 ISLPAFTKECLYYDLSSDKDVLVVSYQVLTGGNFEIDFDITAPDGSVIVTERQKKHSDFLLKSFGIGKYTFCLSNNYGT-SPKKVEITLEKEKEIVSSHESKEDIIAN---NAIEEIDRNLNKITKTMDYLRAREWRNMYTVSSTESRLTWLSLLIMGVMVGISIVQ 191

[Close]

BLAST of EMP24 vs. Ensembl Yeast
Match: ERP2 (Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; role in misfolded protein quality control; forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP2 has a paralog, ERP4, that arose from the whole genome duplication [Source:SGD;Acc:S000000005])

HSP 1 Score: 51.9878 bits (123), Expect = 2.285e-8
Identity = 37/154 (24.03%), Postives = 70/154 (45.45%), Query Frame = -1

Query:  477 ITVDANSDEC-FFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKL--EDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLW 929
            I++ A S EC ++D +     + + ++V+ GG  +ID  +  PDG  I +  ++    +   +   G Y +CFSN   T   K+    + + ++     E   D + + +   + V E+  +L  +     YL  RE  + S   +T SR+  W
Sbjct:   34 ISLPAFSKECLYYDMVTEDDSLAVGYQVLTGGNFEIDFDITAPDGSVITSEKQKKYSDFLLKSFGVGKYTFCFSNNYGTALKKV---EITLEKEKTLTDEHEADVNNDDIIANNAVEEIDRNLNKITKTLNYLRAREWRNMSTVNSTESRLT-W 183

[Close]

BLAST of EMP24 vs. Ensembl Yeast
Match: ERP6 (Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication [Source:SGD;Acc:S000002970])

HSP 1 Score: 47.3654 bits (111), Expect = 8.622e-7
Identity = 45/201 (22.39%), Postives = 85/201 (42.29%), Query Frame = -1

Query:  432 IFLILCHLIFKIKGYYITVDANSDECFF-----DRLVSGAKMMLMFEVVEGGFLD------------IDVK-VFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKI-IMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 977
            IFL    L F++  +Y        +CF      D L+ G+  +   EV +    D            IDV+ VF  + + ++     S G ++F A   G YK C  ++++    K      ++         +M        L   V  L++ +++++ +Q+ +  RE   R I+E+ NSR + W+  +  LL+ + + Q
Sbjct:    6 IFLAFVLLPFRVSAFYFYGYGGDRKCFLKELSKDTLLKGSYNL---EVYDDKLADYALPSYNDYGIVIDVEEVFDNNHRVVHQQGSPS-GDFSFLALESGEYKICLQSRVNNWVGKTKTKLEIEFEVGFEAMLDMQRKETLESLHGKVSILNSKIVDIRREQQLMREREESFRDISESVNSRAMWWTVTQVTLLIIICVWQ 202

[Close]

BLAST of EMP24 vs. Ensembl Nematostella
Match: EDO31547 (Transmembrane emp24 domain-containing protein [Source:UniProtKB/Swiss-Prot;Acc:A7SXK3])

HSP 1 Score: 236.113 bits (601), Expect = 4.107e-77
Identity = 102/168 (60.71%), Postives = 139/168 (82.74%), Query Frame = -1

Query:  432 YYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 935
            Y+IT+DA+ +ECF D++ SG KM L+FEV EGGFLDIDVK+ GPD K IY+G+RE++GKY F+AH+DG Y YCFSNKMSTMTPK++ FSMD+ + P++  + +   H +KL +MV +LS ++  VKH+QEY+EVRER+HRSIN+NTNSRVV WS FE+++L++MT+GQ
Sbjct:   23 YFITIDAHGEECFHDKVTSGTKMGLIFEVAEGGFLDIDVKIIGPDQKVIYSGERETSGKYTFAAHMDGTYNYCFSNKMSTMTPKVLKFSMDIGEAPKDTSKEDNAGH-DKLSEMVSQLSEAMTGVKHEQEYMEVRERIHRSINDNTNSRVVWWSFFESLVLVAMTLGQ 189

[Close]

BLAST of EMP24 vs. Ensembl Nematostella
Match: EDO42051 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2I7])

HSP 1 Score: 87.4261 bits (215), Expect = 3.134e-20
Identity = 49/165 (29.70%), Postives = 86/165 (52.12%), Query Frame = -1

Query:  432 VDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEM-NTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 923
            ++ N+ +CF++ L   AK  L F+V+ GG  D+DV ++  + K +Y+  ++    + F+    GIYK+CFSN+ ST++ K + F     ++ +  K M N      ++E     +  +L      Q +  +RE   R + E  N RV  WS  E+VL++ + I Q
Sbjct:   30 LEDNARQCFYEELKKDAKATLEFQVITGGNYDVDVILYDINEKELYSVQKKQYDSHTFTVEQPGIYKFCFSNEFSTISHKTVYFDFQSGEETQLTKTMGNHHTALTQMETACVTIHENLKIAIDYQTHHRLRESQGRDMAEYLNERVQWWSVGESVLIVIVGICQ 194

[Close]

BLAST of EMP24 vs. Ensembl Nematostella
Match: EDO35763 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SKI6])

HSP 1 Score: 65.4698 bits (158), Expect = 1.873e-12
Identity = 47/177 (26.55%), Postives = 83/177 (46.89%), Query Frame = -1

Query:  432 GYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGK-AIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVK-------HQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 938
            G+   VD    +CF++ +     + + ++V+EGG LDI   +  P GK AI  G R ++  ++     +G Y++CF N  ST+  K I   + V  +    K +     +N  ED+      +L NV+       H Q ++         + ++ +SRV  WS  ++V L+ + I Q
Sbjct:   34 GFTFRVDPGKRDCFYETIKRNVTLDVEYQVIEGGDLDIGFTLQAPSGKIAIEDGHR-TDEAHSLVTEEEGEYEFCFDNTFSTVASKAIFADLGVDFEDDIQKMIGI-KDENLEEDLHDRFVRTLENVRVDLDKAEHIQNFISSAAARDFRLAKSKDSRVFWWSLIQSVALIGVAIVQ 208

[Close]

BLAST of EMP24 vs. Ensembl Nematostella
Match: EDO49537 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGJ6])

HSP 1 Score: 60.4622 bits (145), Expect = 6.882e-11
Identity = 46/186 (24.73%), Postives = 80/186 (43.01%), Query Frame = -1

Query:  450 LILC----HLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMS-TMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLN-------VKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLL 971
            LI C    HL F + GY         ECF+  ++   +  L F  + G    +DV +  P G  IY  D++    + F+  ++G Y+ CFSN    T+   ++ F   ++    E   + T          + +L  SL         ++  Q Y  +RE   R   E+ NS+V  WS  ++++++
Sbjct:   17 LIYCSFARHLQFHLSGY---------ECFYQDILEKTECSLEFAPITGNDPKVDVTLEDPSGNVIYRVDKKDYDLHKFTTTMNGTYQVCFSNVYDFTIGENLVYFDF-IAGVEDEFGTIGTAGETGPASKALTQLETSLSTIHENMNLIERYQNYYRLREANGRIAAESLNSKVQWWSIGQSIIVV 192

[Close]

BLAST of EMP24 vs. Ensembl Nematostella
Match: EDO35953 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SK51])

HSP 1 Score: 53.5286 bits (127), Expect = 2.250e-8
Identity = 44/204 (21.57%), Postives = 88/204 (43.14%), Query Frame = -1

Query:  432 MRNISIFLILCHLIFKIKGYYITVDANSDECFFDR-----LVSGAKMMLMFEVVEGGFLD------IDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCF---SNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDM---VYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 992
            MR +     + +++  ++  Y  +     +CF +      +V G     +++     FL       + V+V  P+GK I +    S G++ F++H  G +  C    S + S      +   +D+      N      A ++KL ++   + +L + +  +  +Q Y   RE   R  +E+TN RV+ W+  + V+LL+    Q
Sbjct:    1 MRLVVSLAFIGYMLGNVQALYFHMGETEKKCFIEEIPDETMVIGKYKTQLYDENIKDFLPSSPGIGMHVEVKDPNGKIIMSKYYSSEGRFTFTSHTPGEHVICLHSNSTRWSLWAGGRLRIHLDIQVGEHANDYAQIAA-KDKLTELQLRIRQLLDQVEQISKEQNYQRFREERFRMTSESTNQRVLWWALAQTVILLATGFWQ 203

[Close]

BLAST of EMP24 vs. Ensembl Medaka
Match: tmed2 (transmembrane emp24 domain-containing protein 2 [Source:NCBI gene;Acc:101161906])

HSP 1 Score: 258.455 bits (659), Expect = 2.143e-85
Identity = 111/182 (60.99%), Postives = 154/182 (84.62%), Query Frame = -1

Query:  432 IFLILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 977
            + ++L  L     GY++++DA+++ECF++R+ SG KM LMFEV EGGFLDIDV++ GPDGK IY GDRES+GKY+ +AH+DG YK+CFSNKMSTMTPKI+MF++D+ + P+  + M T+AHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HR+IN+NTNSRVVLWS FEA++L++MT+GQ
Sbjct:    7 LVVLLAVLTATASGYFVSIDAHAEECFYERVNSGTKMGLMFEVAEGGFLDIDVEIMGPDGKQIYKGDRESSGKYSVAAHMDGTYKFCFSNKMSTMTPKIVMFTIDIGEAPK-GQNMETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQ 187

[Close]

BLAST of EMP24 vs. Ensembl Medaka
Match: tmed2 (transmembrane emp24 domain-containing protein 2 [Source:NCBI gene;Acc:101161906])

HSP 1 Score: 253.832 bits (647), Expect = 1.592e-83
Identity = 112/187 (59.89%), Postives = 155/187 (82.89%), Query Frame = -1

Query:  432 IFLILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPR----ENKEMNT-DAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 977
            + ++L  L     GY++++DA+++ECF++R+ SG KM LMFEV EGGFLDIDV++ GPDGK IY GDRES+GKY+ +AH+DG YK+CFSNKMSTMTPKI+MF++D+ + P+    E +  N+ DAHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HR+IN+NTNSRVVLWS FEA++L++MT+GQ
Sbjct:    7 LVVLLAVLTATASGYFVSIDAHAEECFYERVNSGTKMGLMFEVAEGGFLDIDVEIMGPDGKQIYKGDRESSGKYSVAAHMDGTYKFCFSNKMSTMTPKIVMFTIDIGEAPKGQNMETEGANSWDAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQ 193

[Close]

BLAST of EMP24 vs. Ensembl Medaka
Match: tmed2 (transmembrane emp24 domain-containing protein 2 [Source:NCBI gene;Acc:101161906])

HSP 1 Score: 215.698 bits (548), Expect = 7.860e-69
Identity = 91/155 (58.71%), Postives = 128/155 (82.58%), Query Frame = -1

Query:  513 IFLILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRS 977
            + ++L  L     GY++++DA+++ECF++R+ SG KM LMFEV EGGFLDIDV++ GPDGK IY GDRES+GKY+ +AH+DG YK+CFSNKMSTMTPKI+MF++D+ + P+  + M T+AHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HR+
Sbjct:    7 LVVLLAVLTATASGYFVSIDAHAEECFYERVNSGTKMGLMFEVAEGGFLDIDVEIMGPDGKQIYKGDRESSGKYSVAAHMDGTYKFCFSNKMSTMTPKIVMFTIDIGEAPK-GQNMETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRA 160

[Close]

Query:  513 IFLILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRS 977
            + ++L  L     GY++++DA+++ECF++R+ SG KM LMFEV EGGFLDIDV++ GPDGK IY GDRES+GKY+ +AH+DG YK+CFSNKMSTMTPKI+MF++D+ + P+  + M T+AHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HR+
Sbjct:    7 LVVLLAVLTATASGYFVSIDAHAEECFYERVNSGTKMGLMFEVAEGGFLDIDVEIMGPDGKQIYKGDRESSGKYSVAAHMDGTYKFCFSNKMSTMTPKIVMFTIDIGEAPK-GQNMETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRA 160

[Close]

Query:  513 IFLILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRS 977
            + ++L  L     GY++++DA+++ECF++R+ SG KM LMFEV EGGFLDIDV++ GPDGK IY GDRES+GKY+ +AH+DG YK+CFSNKMSTMTPKI+MF++D+ + P+  + M T+AHQNKLE+M+ EL+ ++  VKH+QEY+EVRER+HR+
Sbjct:    7 LVVLLAVLTATASGYFVSIDAHAEECFYERVNSGTKMGLMFEVAEGGFLDIDVEIMGPDGKQIYKGDRESSGKYSVAAHMDGTYKFCFSNKMSTMTPKIVMFTIDIGEAPK-GQNMETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRA 160

[Close]

BLAST of EMP24 vs. Planmine SMEST
Match: SMESG000069470.1 (SMESG000069470.1)

HSP 1 Score: 389.808 bits (1000), Expect = 2.584e-137
Identity = 187/187 (100.00%), Postives = 187/187 (100.00%), Query Frame = -1

Query:  432 MRNISIFLILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 992
            MRNISIFLILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ
Sbjct:    1 MRNISIFLILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 187

[Close]

BLAST of EMP24 vs. Planmine SMEST
Match: SMESG000069470.1 (SMESG000069470.1)

HSP 1 Score: 300.442 bits (768), Expect = 1.018e-102
Identity = 143/143 (100.00%), Postives = 143/143 (100.00%), Query Frame = -1

Query:  432 MFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 860
            MFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ
Sbjct:    1 MFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 143

[Close]

BLAST of EMP24 vs. Planmine SMEST
Match: SMESG000026239.1 (SMESG000026239.1)

HSP 1 Score: 193.356 bits (490), Expect = 3.540e-60
Identity = 91/182 (50.00%), Postives = 128/182 (70.33%), Query Frame = -1

Query:  432 IFLILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 977
            IF +L  L+     ++I+V  N +ECFFDR+     M+L++EV EGG LDID KV  P G  +Y+  ++S+ K+ F   VDGIYKYCFSN MS++T KI+MFS+D     +++   + D+H +KLE+MV EL  +L  VKH+QEY+E RE++HR INENTNSRV+LW+ FEA +L+ M+ GQ
Sbjct:    4 IFCVLFILLHSALSFFISVSPNVEECFFDRIKPDTDMLLLYEVAEGGSLDIDAKVLDPSGSILYSAVKKSDDKFTFRTKVDGIYKYCFSNTMSSVTNKILMFSLDTISPAKKD---SPDSH-SKLEEMVSELDKALTTVKHEQEYMETREKIHRKINENTNSRVLLWAIFEACVLIIMSAGQ 181

[Close]

BLAST of EMP24 vs. Planmine SMEST
Match: SMESG000025949.1 (SMESG000025949.1)

HSP 1 Score: 158.303 bits (399), Expect = 1.237e-46
Identity = 70/169 (41.42%), Postives = 113/169 (66.86%), Query Frame = -1

Query:  432 GYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 938
             +YI ++    ECFFD+  +  +++LMFEV+EGG++DI++++FGPD K IY GDRESNGKY F+ +  G Y+YCFSN M+    KI++F+MD+     + KE      Q+ + +++ E+S  + +VKH+ ++L+ R +VH  INE TN  ++LW   E   L+ +++GQ
Sbjct:   20 SHYIIIEPKETECFFDKANTNDRIVLMFEVIEGGYMDINIQLFGPDKKVIYQGDRESNGKYTFATNQPGKYRYCFSNSMNDKDRKIVLFNMDIITPKVDQKE-----PQDSMINLISEMSVRIFDVKHEVQFLKARHQVHYEINEKTNKYILLWVIIEISCLICLSLGQ 183

[Close]

BLAST of EMP24 vs. Planmine SMEST
Match: SMESG000078201.1 (SMESG000078201.1)

HSP 1 Score: 106.686 bits (265), Expect = 5.336e-27
Identity = 60/186 (32.26%), Postives = 98/186 (52.69%), Query Frame = -1

Query:  432 FLILCHLIF--KIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVEGGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTMTPKIIMFSMDVSQKPRENKEMNTDAHQNK---LEDMVYELSNSLLNVKHQQEYLEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQ 974
            F +  H+I    +  + I ++ NS+EC FD      K+   +EV+ GGFLDI+ K+  P        D+ S G Y       G+Y +C  NK  +   K + F++    +P    E N  + ++K   LE++V++LS  + +VK + EY   RE++H   N + N   +LW  FE VL+++M+IGQ
Sbjct:    6 FALFVHIIIINNLSSFEINLNPNSEECLFDYFNKSDKVEFSYEVINGGFLDIESKLLSPMKVEFMRRDKGSYGVYDLVIEQSGLYYFCLINKEGSQVEKTVRFNL--FSEPHYRWERNITSRESKNSDLEEIVHDLSFLMHSVKAESEYSLTREQIHDQANRSANRHFLLWGLFEMVLMVAMSIGQ 189

The following BLAST results are available for this feature:

BLAST of EMP24 vs. Ensembl Human
BLAST of EMP24 vs. Ensembl Celegans
BLAST of EMP24 vs. Ensembl Fly
BLAST of EMP24 vs. Ensembl Zebrafish
BLAST of EMP24 vs. Ensembl Xenopus
BLAST of EMP24 vs. Ensembl Mouse
BLAST of EMP24 vs. UniProt/SwissProt
BLAST of EMP24 vs. TrEMBL
BLAST of EMP24 vs. Ensembl Cavefish
BLAST of EMP24 vs. Ensembl Sea Lamprey
BLAST of EMP24 vs. Ensembl Yeast
BLAST of EMP24 vs. Ensembl Nematostella
BLAST of EMP24 vs. Ensembl Medaka
BLAST of EMP24 vs. Planmine SMEST

BLAST of EMP24 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
TMED2	7.852e-84	63.91	transmembrane p24 trafficking protein 2 [Source:HG...	[more]
TMED2	1.068e-64	60.14	transmembrane p24 trafficking protein 2 [Source:HG...	[more]
TMED2	2.943e-45	63.11	transmembrane p24 trafficking protein 2 [Source:HG...	[more]
TMED7	3.009e-28	34.16	transmembrane p24 trafficking protein 7 [Source:HG...	[more]
TMED7-TICAM2	2.159e-21	31.94	TMED7-TICAM2 readthrough [Source:HGNC Symbol;Acc:H...	[more]

BLAST of EMP24 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3


Match Name	E-value	Identity	Description
sel-9	6.916e-77	57.98	Suppressor/enhancer of lin-12 protein 9 [Source:U...	[more]
F57B10.5	3.021e-21	29.01	pep chromosome:WBcel235:I:6562699:6563660:1 gene:W...	[more]
Y60A3A.9	4.410e-7	21.47	pep chromosome:WBcel235:V:19946691:19949093:-1 gen...	[more]

BLAST of EMP24 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
CHOp24	9.715e-61	49.71	gene:FBgn0029709 transcript:FBtr0343566	[more]
CHOp24	9.715e-61	49.71	gene:FBgn0029709 transcript:FBtr0070675	[more]
CG9308	7.582e-33	34.64	gene:FBgn0034681 transcript:FBtr0071749	[more]
p24-1	2.807e-17	25.82	gene:FBgn0030341 transcript:FBtr0073604	[more]
p24-1	2.807e-17	25.82	gene:FBgn0030341 transcript:FBtr0334455	[more]

BLAST of EMP24 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
tmed2	2.349e-87	61.54	transmembrane p24 trafficking protein 2 [Source:ZF...	[more]
tmed2	6.525e-85	59.79	transmembrane p24 trafficking protein 2 [Source:ZF...	[more]
tmed7	2.782e-26	33.54	transmembrane p24 trafficking protein 7 [Source:ZF...	[more]
tmed7	2.782e-26	33.54	transmembrane p24 trafficking protein 7 [Source:ZF...	[more]
tmed7	2.782e-26	33.54	transmembrane p24 trafficking protein 7 [Source:ZF...	[more]

BLAST of EMP24 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
tmed2	8.562e-84	60.34	transmembrane p24 trafficking protein 2 [Source:NC...	[more]
tmed2	1.670e-81	58.60	transmembrane p24 trafficking protein 2 [Source:NC...	[more]
serinc2	9.795e-28	34.16	serine incorporator 2 [Source:Xenbase;Acc:XB-GENE-...	[more]
serinc2	1.740e-27	34.16	serine incorporator 2 [Source:Xenbase;Acc:XB-GENE-...	[more]
tmed5	9.275e-18	26.24	transmembrane p24 trafficking protein 5 [Source:NC...	[more]

BLAST of EMP24 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Tmed2	6.208e-84	63.91	transmembrane p24 trafficking protein 2 [Source:MG...	[more]
Tmed2	3.419e-60	63.57	transmembrane p24 trafficking protein 2 [Source:MG...	[more]
Tmed2	1.681e-35	58.59	transmembrane p24 trafficking protein 2 [Source:MG...	[more]
Tmed7	3.282e-28	33.54	transmembrane p24 trafficking protein 7 [Source:MG...	[more]
Tmed7	1.467e-21	31.94	transmembrane p24 trafficking protein 7 [Source:MG...	[more]

BLAST of EMP24 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P49020\|TMED2_CRIGR	3.402e-83	63.91	Transmembrane emp24 domain-containing protein 2 (F...	[more]
sp\|Q63524\|TMED2_RAT	3.771e-83	63.91	Transmembrane emp24 domain-containing protein 2 OS...	[more]
sp\|Q15363\|TMED2_HUMAN	3.771e-83	63.91	Transmembrane emp24 domain-containing protein 2 OS...	[more]
sp\|Q9R0Q3\|TMED2_MOUSE	4.343e-83	63.91	Transmembrane emp24 domain-containing protein 2 OS...	[more]
sp\|O17528\|TMED2_CAEEL	8.797e-76	57.98	Suppressor/enhancer of lin-12 protein 9 OS=Caenorh...	[more]

BLAST of EMP24 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
V9XRM7	9.290e-134	100.00	EMP24 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1	[more]
A0A1I8HUD8	5.022e-90	67.60	GOLD domain-containing protein OS=Macrostomum lign...	[more]
C3XVK2	2.902e-87	62.77	GOLD domain-containing protein OS=Branchiostoma fl...	[more]
A0A2C9JWM8	2.111e-85	63.98	GOLD domain-containing protein OS=Biomphalaria gla...	[more]
X1ZJR4	5.874e-85	65.38	GOLD domain-containing protein OS=Capitella teleta...	[more]

BLAST of EMP24 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
tmed2	4.057e-84	65.09	transmembrane emp24 domain-containing protein 2 [S...	[more]
TMED2	1.552e-82	59.26	transmembrane emp24 domain-containing protein 2 [S...	[more]
TMED2	1.552e-82	59.26	transmembrane emp24 domain-containing protein 2 [S...	[more]
TMED2	7.105e-82	58.95	transmembrane emp24 domain-containing protein 2 [S...	[more]
tmed7	2.928e-27	31.05	transmembrane p24 trafficking protein 7 [Source:ZF...	[more]

BLAST of EMP24 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
tmed2	4.195e-63	63.08	transmembrane p24 trafficking protein 2 [Source:ZF...	[more]
tmed7	1.845e-21	29.76	transmembrane p24 trafficking protein 7 [Source:ZF...	[more]
tmed3	2.248e-21	30.86	transmembrane p24 trafficking protein 3 [Source:ZF...	[more]
tmed5	1.543e-16	27.75	transmembrane p24 trafficking protein 5 [Source:ZF...	[more]
tmed5	4.527e-16	29.17	transmembrane p24 trafficking protein 5 [Source:ZF...	[more]

BLAST of EMP24 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 4


Match Name	E-value	Identity	Description
EMP24	1.914e-18	27.46	Component of the p24 complex; role in misfolded pr...	[more]
ERP4	2.402e-9	23.35	Member of the p24 family involved in ER to Golgi t...	[more]
ERP2	2.285e-8	24.03	Member of the p24 family involved in ER to Golgi t...	[more]
ERP6	8.622e-7	22.39	Member of the p24 family involved in ER to Golgi t...	[more]

BLAST of EMP24 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO31547	4.107e-77	60.71	Transmembrane emp24 domain-containing protein [So...	[more]
EDO42051	3.134e-20	29.70	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO35763	1.873e-12	26.55	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO49537	6.882e-11	24.73	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO35953	2.250e-8	21.57	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of EMP24 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
tmed2	2.143e-85	60.99	transmembrane emp24 domain-containing protein 2 [S...	[more]
tmed2	1.592e-83	59.89	transmembrane emp24 domain-containing protein 2 [S...	[more]
tmed2	7.860e-69	58.71	transmembrane emp24 domain-containing protein 2 [S...	[more]
tmed2	7.860e-69	58.71	transmembrane emp24 domain-containing protein 2 [S...	[more]
tmed2	7.860e-69	58.71	transmembrane emp24 domain-containing protein 2 [S...	[more]

BLAST of EMP24 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000069470.1	2.584e-137	100.00	SMESG000069470.1	[more]
SMESG000069470.1	1.018e-102	100.00	SMESG000069470.1	[more]
SMESG000026239.1	3.540e-60	50.00	SMESG000026239.1	[more]
SMESG000025949.1	1.237e-46	41.42	SMESG000025949.1	[more]
SMESG000078201.1	5.336e-27	32.26	SMESG000078201.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Cross References

External references for this transcript


Database	Accession
SmedGD GBrowse	SMED30031611
GenBank	AHD24743.1

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30031611 ID=SMED30031611|Name=EMP24|organism=Schmidtea mediterranea sexual|type=transcript|length=1139bp
TTTTTTGAAAAAATAATAAAATACACTTTCATCGATTATTTTCATCAAAT
CTAAAAAAACAATGAAAATAAAGATTGACAAGATAGAATCAAAATACTTG
GTAATATTAAAAAATGAATATTAATAATCTTAGTATAAACAAAAAGCAAC
GCAGCATAAAACATTTTCAAGTTTCAATATTAAAATACAAATCACAACAC
ATCTTAAATCCGAGTTCCCAAACCGATACAGAAATATTAGGTGGAATTTC
AACATAATAAATTAAAAACAAAAATTACACAATTTCATACAATAAATCCA
ATTTTAATGAAATTAATTTAAATTGTTATTATTACTAATGATTTGAGACA
TCAGATATGAAAAAAGACAATGTATTGTAACAATAATTACACAACTCGGC
GAACTTCAAAAAACCTTCTTAAATAATAAACTTGGCCAATTGTCATAGAT
AATAACAAAACTGCTTCAAAAGCAGACCATAAGACTACTCTTGAATTTGT
GTTTTCATTTATTGATCGATGAACCCTTTCTCTGACTTCAAGATATTCTT
GCTGATGTTTTACATTTAAAAGGGAATTACTCAATTCATATACCATATCT
TCTAATTTATTTTGATGAGCATCTGTATTCATTTCTTTATTTTCTCTTGG
CTTTTGACTTACATCCATAGAAAACATTATTATTTTAGGTGTCATTGTGC
TCATTTTATTACTAAAGCAATATTTATAAATCCCGTCTACATGAGCTGAG
AATGCGTATTTACCATTGGATTCTCTATCTCCTGTGTAAATTGCCTTACC
ATCCGGTCCAAATACTTTAACGTCGATGTCTAAAAATCCACCTTCAACAA
CTTCAAACATTAACATCATTTTGGCACCAGAAACTAATCTGTCAAAAAAA
CATTCATCTGAATTTGCATCTACGGTGATATAGTAGCCTTTAATTTTAAA
AATTAAGTGGCATAATATTAGGAAAATAGAAATATTTCGCATATTGCCAC
GACAAATTTTAATTATTACCACTGGTGTAAAGAAACAAATAGTTAAATTA
AAACACAAAGTAACTAATTATGACTTGGATGAAACATTAAGTCACAATAT
TTAGCAAATTGTATTTAGTAATTTGTAAAAATTAAAATG

protein sequence of SMED30031611-orf-1

>SMED30031611-orf-1 ID=SMED30031611-orf-1|Name=SMED30031611-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=202bp
MRNISIFLILCHLIFKIKGYYITVDANSDECFFDRLVSGAKMMLMFEVVE
GGFLDIDVKVFGPDGKAIYTGDRESNGKYAFSAHVDGIYKYCFSNKMSTM
TPKIIMFSMDVSQKPRENKEMNTDAHQNKLEDMVYELSNSLLNVKHQQEY
LEVRERVHRSINENTNSRVVLWSAFEAVLLLSMTIGQVYYLRRFFEVRRV
V*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0002032	epidermal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR015720	TMP21-related
IPR036598	GOLD_dom_sf
IPR009038	GOLD_dom

Vocabulary: **biological process**
Term	Definition
GO:0006810	transport

Vocabulary: **cellular component**
Term	Definition
GO:0016021	integral component of membrane

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009038	GOLD domain	SMART	SM01190	EMP24_GP25L_2	coord: 19..196 e-value: 1.1E-66 score: 237.6
IPR009038	GOLD domain	PFAM	PF01105	EMP24_GP25L	coord: 20..196 e-value: 4.3E-46 score: 157.2
IPR009038	GOLD domain	PROSITE	PS50866	GOLD	coord: 29..111 score: 20.397
IPR015720	TMP21-related	PANTHER	PTHR22811	TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN	coord: 8..196
None	No IPR available	PANTHER	PTHR22811:SF31	TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN 2	coord: 8..196
None	No IPR available	SIGNALP_EUK	SignalP-noTM	SignalP-noTM	coord: 1..19 score: 0.802
None	No IPR available	TMHMM	TMhelix		coord: 169..191
IPR036598	GOLD domain superfamily	SUPERFAMILY	SSF101576	Supernatant protein factor (SPF), C-terminal domain	coord: 28..119