TMEM189_B_dmain domain-containing protein

Overview
NameTMEM189_B_dmain domain-containing protein
Smed IDSMED30031558
Length (bp)1524
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of TMEM189_B_dmain domain-containing protein (SMED30031558) t-SNE clustered cells

Violin plots show distribution of expression levels for TMEM189_B_dmain domain-containing protein (SMED30031558) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of TMEM189_B_dmain domain-containing protein (SMED30031558) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for TMEM189_B_dmain domain-containing protein (SMED30031558) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30031558

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pigment cellSMED30031558h1SMcG0014850 SmedASXL_007047SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30031558h1SMcG0014850 dd_1274dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
neuronSMED30031558h1SMcG0014850 dd_1274dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
parenchymal cellSMED30031558h1SMcG0014850 dd_Smed_v4_3344_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031558h1SMcG0014850 Contig26238uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031558h1SMcG0014850 Contig26238newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30031558h1SMcG0014850 dd_Smed_v4_1274_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30031558h1SMcG0014850 dd_Smed_v4_1274_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031558h1SMcG0014850 dd_Smed_v4_1274_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30031558h1SMcG0014850 dd_Smed_v4_3344_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Human
Match: TMEM189 (transmembrane protein 189 [Source:HGNC Symbol;Acc:HGNC:16735])

HSP 1 Score: 306.99 bits (785), Expect = 2.461e-101
Identity = 146/248 (58.87%), Postives = 181/248 (72.98%), Query Frame = 1 
Query:  271 KFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQK 1014
            ++G+QHAGA+ELAA YS GKR QE    ++   L+ +NL+ L++         ++     G ++AD LSGLVHW  DTWGSVE P++G   IRPFREHHIDPT+ITRHDFIETNGDN    +     +A+K R +  E L  +  Y +  F++ L +F +FTNQ HKWSHTY+GLPRWVT LQDWH+ILPRKHHR+HHVSPHETY+CITTGWLN+PLEKI FWR LE LI+  TG KPR+DDM WAQK
Sbjct:   23 RWGAQHAGARELAALYSPGKRLQEWCSVILCFSLIAHNLVHLLLLARWEDTPLVILGVVAGALIADFLSGLVHWGADTWGSVELPIVGKAFIRPFREHHIDPTAITRHDFIETNGDNCLVTLLPLLNMAYKFRTHSPEAL--EQLYPWECFVFCLIIFGTFTNQIHKWSHTYFGLPRWVTLLQDWHVILPRKHHRIHHVSPHETYFCITTGWLNYPLEKIGFWRRLEDLIQGLTGEKPRADDMKWAQK 268
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Human
Match: TMEM189 (transmembrane protein 189 [Source:HGNC Symbol;Acc:HGNC:16735])

HSP 1 Score: 298.13 bits (762), Expect = 5.924e-98
Identity = 145/248 (58.47%), Postives = 179/248 (72.18%), Query Frame = 1 
Query:  271 KFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQK 1014
            ++G+QHAGA+ELAA YS GKR QE    ++   L+ +NL+ L++         ++     G ++AD LSGLVHW  DTWGSVE P+     IRPFREHHIDPT+ITRHDFIETNGDN    +     +A+K R +  E L  +  Y +  F++ L +F +FTNQ HKWSHTY+GLPRWVT LQDWH+ILPRKHHR+HHVSPHETY+CITTGWLN+PLEKI FWR LE LI+  TG KPR+DDM WAQK
Sbjct:   23 RWGAQHAGARELAALYSPGKRLQEWCSVILCFSLIAHNLVHLLLLARWEDTPLVILGVVAGALIADFLSGLVHWGADTWGSVELPI---AFIRPFREHHIDPTAITRHDFIETNGDNCLVTLLPLLNMAYKFRTHSPEAL--EQLYPWECFVFCLIIFGTFTNQIHKWSHTYFGLPRWVTLLQDWHVILPRKHHRIHHVSPHETYFCITTGWLNYPLEKIGFWRRLEDLIQGLTGEKPRADDMKWAQK 265
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Human
Match: TMEM189 (transmembrane protein 189 [Source:HGNC Symbol;Acc:HGNC:16735])

HSP 1 Score: 273.478 bits (698), Expect = 1.864e-89
Identity = 126/191 (65.97%), Postives = 148/191 (77.49%), Query Frame = 1 
Query:  442 AFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQK 1014
            A  G ++AD LSGLVHW  DTWGSVE P++G   IRPFREHHIDPT+ITRHDFIETNGDN    +     +A+K R +  E L  +  Y +  F++ L +F +FTNQ HKWSHTY+GLPRWVT LQDWH+ILPRKHHR+HHVSPHETY+CITTGWLN+PLEKI FWR LE LI+  TG KPR+DDM WAQK
Sbjct:    5 AIAGALIADFLSGLVHWGADTWGSVELPIVGKAFIRPFREHHIDPTAITRHDFIETNGDNCLVTLLPLLNMAYKFRTHSPEAL--EQLYPWECFVFCLIIFGTFTNQIHKWSHTYFGLPRWVTLLQDWHVILPRKHHRIHHVSPHETYFCITTGWLNYPLEKIGFWRRLEDLIQGLTGEKPRADDMKWAQK 193
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Human
Match: TMEM189-UBE2V1 (TMEM189-UBE2V1 readthrough [Source:HGNC Symbol;Acc:HGNC:33521])

HSP 1 Score: 253.447 bits (646), Expect = 2.972e-79
Identity = 125/235 (53.19%), Postives = 161/235 (68.51%), Query Frame = 1 
Query:  271 KFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATG 975
            ++G+QHAGA+ELAA YS GKR QE    ++   L+ +NL+ L++         ++     G ++AD LSGLVHW  DTWGSVE P++G   IRPFREHHIDPT+ITRHDFIETNGDN    +     +A+K R +  E L  +  Y +  F++ L +F +FTNQ HKWSHTY+GLPRWVT LQDWH+ILPRKHHR+HHVSPHETY+CITTG     ++  + +R LE L E   G
Sbjct:   23 RWGAQHAGARELAALYSPGKRLQEWCSVILCFSLIAHNLVHLLLLARWEDTPLVILGVVAGALIADFLSGLVHWGADTWGSVELPIVGKAFIRPFREHHIDPTAITRHDFIETNGDNCLVTLLPLLNMAYKFRTHSPEAL--EQLYPWECFVFCLIIFGTFTNQIHKWSHTYFGLPRWVTLLQDWHVILPRKHHRIHHVSPHETYFCITTG-----VKVPRNFRLLEELEEGQKG 250
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Celegans
Match: Y53C10A.5 (pep chromosome:WBcel235:I:11998781:12002643:1 gene:WBGene00013136.1 transcript:Y53C10A.5.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y53C10A.5)

HSP 1 Score: 265.774 bits (678), Expect = 4.961e-85
Identity = 133/278 (47.84%), Postives = 179/278 (64.39%), Query Frame = 1 
Query:  196 KEIGMPEISYQVSDDINKDNVE-----RIYKFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQK 1014
            +E+ +  +   + DD N + VE     +  ++G QHAGAK+LA+ YS  KR QE V       L     I L++++  S W S+L  A  GI+ AD  SGLVHW  DT+GSVE    G   IRPFREHH+DPT+ITRHD +E NGDN    +    ++ ++   Y  + +    + +F+ +I LL ++V+ TNQ HKWSHTY+GLP WV FLQ  HIILPR HH++HH+SPH  YYCITTGWLNWPLE I FWR +E+++ + TG +PR DD+ WA K
Sbjct:   43 EEVDIDTLPSMLEDDPNGNVVECDLGFKGPRWGPQHAGAKKLASMYSKEKRLQEKVSLFAAIFLFSIVFINLLLSWESSIWVSVLVSAVLGIMTADFASGLVHWAADTFGSVE-TWFGRSFIRPFREHHVDPTAITRHDIVEVNGDNCMLCVGPLLWILYQQMTYQRDAIT--QWATFHWYILLLGIYVALTNQIHKWSHTYFGLPTWVVFLQKAHIILPRSHHKIHHISPHACYYCITTGWLNWPLEYIGFWRKMEWVVTTVTGMQPREDDLKWATK 317
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Celegans
Match: Y53C10A.5 (pep chromosome:WBcel235:I:11998781:12002612:1 gene:WBGene00013136.1 transcript:Y53C10A.5.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y53C10A.5)

HSP 1 Score: 265.774 bits (678), Expect = 4.961e-85
Identity = 133/278 (47.84%), Postives = 179/278 (64.39%), Query Frame = 1 
Query:  196 KEIGMPEISYQVSDDINKDNVE-----RIYKFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQK 1014
            +E+ +  +   + DD N + VE     +  ++G QHAGAK+LA+ YS  KR QE V       L     I L++++  S W S+L  A  GI+ AD  SGLVHW  DT+GSVE    G   IRPFREHH+DPT+ITRHD +E NGDN    +    ++ ++   Y  + +    + +F+ +I LL ++V+ TNQ HKWSHTY+GLP WV FLQ  HIILPR HH++HH+SPH  YYCITTGWLNWPLE I FWR +E+++ + TG +PR DD+ WA K
Sbjct:   43 EEVDIDTLPSMLEDDPNGNVVECDLGFKGPRWGPQHAGAKKLASMYSKEKRLQEKVSLFAAIFLFSIVFINLLLSWESSIWVSVLVSAVLGIMTADFASGLVHWAADTFGSVE-TWFGRSFIRPFREHHVDPTAITRHDIVEVNGDNCMLCVGPLLWILYQQMTYQRDAIT--QWATFHWYILLLGIYVALTNQIHKWSHTYFGLPTWVVFLQKAHIILPRSHHKIHHISPHACYYCITTGWLNWPLEYIGFWRKMEWVVTTVTGMQPREDDLKWATK 317
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Fly
Match: Kua (gene:FBgn0032850 transcript:FBtr0331617)

HSP 1 Score: 300.056 bits (767), Expect = 2.086e-98
Identity = 142/248 (57.26%), Postives = 173/248 (69.76%), Query Frame = 1 
Query:  271 KFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQK 1014
            ++G Q+ GA+ELA  YS GKR QE +C      LM+ NL L++ +    +       A CGII AD  SGLVHW  DTWGSV+ P+IG   +RPFREHH+DPTSITRHDFIETNGDN    IP   YLA     Y       Q ++ + A+++L S+FV+ TNQ HKWSHTY+GLPRWV  LQ  HIILPRKHHR+HHV+PHETY+CITTGWLNWPLE+I+FW   E +IE  TG KPR DD+ WA+K
Sbjct:   63 RWGPQNKGAQELALLYSPGKRAQEIICVYTCIGLMIINLALIVRHLRLERISVAFLSALCGIITADFASGLVHWAADTWGSVDIPMIGKNFLRPFREHHLDPTSITRHDFIETNGDNFMVGIPILGYLAHYF--YIRTPSEIQQHFGWIAYVFLCSIFVAMTNQIHKWSHTYWGLPRWVLLLQSCHIILPRKHHRIHHVAPHETYFCITTGWLNWPLERIRFWSTFELIIEHFTGLKPRDDDLKWAKK 308
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Fly
Match: Kua (gene:FBgn0032850 transcript:FBtr0331616)

HSP 1 Score: 300.056 bits (767), Expect = 2.086e-98
Identity = 142/248 (57.26%), Postives = 173/248 (69.76%), Query Frame = 1 
Query:  271 KFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQK 1014
            ++G Q+ GA+ELA  YS GKR QE +C      LM+ NL L++ +    +       A CGII AD  SGLVHW  DTWGSV+ P+IG   +RPFREHH+DPTSITRHDFIETNGDN    IP   YLA     Y       Q ++ + A+++L S+FV+ TNQ HKWSHTY+GLPRWV  LQ  HIILPRKHHR+HHV+PHETY+CITTGWLNWPLE+I+FW   E +IE  TG KPR DD+ WA+K
Sbjct:   63 RWGPQNKGAQELALLYSPGKRAQEIICVYTCIGLMIINLALIVRHLRLERISVAFLSALCGIITADFASGLVHWAADTWGSVDIPMIGKNFLRPFREHHLDPTSITRHDFIETNGDNFMVGIPILGYLAHYF--YIRTPSEIQQHFGWIAYVFLCSIFVAMTNQIHKWSHTYWGLPRWVLLLQSCHIILPRKHHRIHHVAPHETYFCITTGWLNWPLERIRFWSTFELIIEHFTGLKPRDDDLKWAKK 308
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Fly
Match: Kua (gene:FBgn0032850 transcript:FBtr0081336)

HSP 1 Score: 300.056 bits (767), Expect = 2.086e-98
Identity = 142/248 (57.26%), Postives = 173/248 (69.76%), Query Frame = 1 
Query:  271 KFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQK 1014
            ++G Q+ GA+ELA  YS GKR QE +C      LM+ NL L++ +    +       A CGII AD  SGLVHW  DTWGSV+ P+IG   +RPFREHH+DPTSITRHDFIETNGDN    IP   YLA     Y       Q ++ + A+++L S+FV+ TNQ HKWSHTY+GLPRWV  LQ  HIILPRKHHR+HHV+PHETY+CITTGWLNWPLE+I+FW   E +IE  TG KPR DD+ WA+K
Sbjct:   63 RWGPQNKGAQELALLYSPGKRAQEIICVYTCIGLMIINLALIVRHLRLERISVAFLSALCGIITADFASGLVHWAADTWGSVDIPMIGKNFLRPFREHHLDPTSITRHDFIETNGDNFMVGIPILGYLAHYF--YIRTPSEIQQHFGWIAYVFLCSIFVAMTNQIHKWSHTYWGLPRWVLLLQSCHIILPRKHHRIHHVAPHETYFCITTGWLNWPLERIRFWSTFELIIEHFTGLKPRDDDLKWAKK 308
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Fly
Match: Kua (gene:FBgn0032850 transcript:FBtr0303230)

HSP 1 Score: 300.056 bits (767), Expect = 2.086e-98
Identity = 142/248 (57.26%), Postives = 173/248 (69.76%), Query Frame = 1 
Query:  271 KFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQK 1014
            ++G Q+ GA+ELA  YS GKR QE +C      LM+ NL L++ +    +       A CGII AD  SGLVHW  DTWGSV+ P+IG   +RPFREHH+DPTSITRHDFIETNGDN    IP   YLA     Y       Q ++ + A+++L S+FV+ TNQ HKWSHTY+GLPRWV  LQ  HIILPRKHHR+HHV+PHETY+CITTGWLNWPLE+I+FW   E +IE  TG KPR DD+ WA+K
Sbjct:   63 RWGPQNKGAQELALLYSPGKRAQEIICVYTCIGLMIINLALIVRHLRLERISVAFLSALCGIITADFASGLVHWAADTWGSVDIPMIGKNFLRPFREHHLDPTSITRHDFIETNGDNFMVGIPILGYLAHYF--YIRTPSEIQQHFGWIAYVFLCSIFVAMTNQIHKWSHTYWGLPRWVLLLQSCHIILPRKHHRIHHVAPHETYFCITTGWLNWPLERIRFWSTFELIIEHFTGLKPRDDDLKWAKK 308
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Zebrafish
Match: si:ch211-212o1.2 (si:ch211-212o1.2 [Source:ZFIN;Acc:ZDB-GENE-041111-310])

HSP 1 Score: 305.449 bits (781), Expect = 1.080e-100
Identity = 143/249 (57.43%), Postives = 180/249 (72.29%), Query Frame = 1 
Query:  271 KFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQKT 1017
            ++G QHAGA++LA  YS GKR QE +C ++   L + NL  L +NF       I+     GI+ AD  SG+VHW  DTWGSVE PVIG   IRPFREHHIDPT+ITRHDFIETNGDN    I   A++A+K   Y  + L     Y +  F++ L+VFV+ TNQ HKW+H+Y+ LPRWVT LQD H++LPRKHHR+HHVSPHETYYCITTGWLN+PL+++ FWR +E+LIE  TG +PRSDD+ WA+KT
Sbjct:   46 RWGPQHAGARQLAKLYSPGKRLQEWICVILCLSLFIVNLSFLFLNFSTVHIYRIIFGIVTGIVTADFASGMVHWGADTWGSVEIPVIGKAFIRPFREHHIDPTAITRHDFIETNGDNCMITILPLAHMAYKFLSYPPDALAES--YPWECFVFALAVFVTMTNQIHKWAHSYFDLPRWVTLLQDCHLVLPRKHHRIHHVSPHETYYCITTGWLNYPLDRVGFWRTMEWLIERLTGQRPRSDDLAWAKKT 292
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Zebrafish
Match: si:ch211-212o1.2 (si:ch211-212o1.2 [Source:ZFIN;Acc:ZDB-GENE-041111-310])

HSP 1 Score: 304.294 bits (778), Expect = 1.313e-100
Identity = 143/249 (57.43%), Postives = 180/249 (72.29%), Query Frame = 1 
Query:  271 KFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQKT 1017
            ++G QHAGA++LA  YS GKR QE +C ++   L + NL  L +NF       I+     GI+ AD  SG+VHW  DTWGSVE PVIG   IRPFREHHIDPT+ITRHDFIETNGDN    I   A++A+K   Y  + L     Y +  F++ L+VFV+ TNQ HKW+H+Y+ LPRWVT LQD H++LPRKHHR+HHVSPHETYYCITTGWLN+PL+++ FWR +E+LIE  TG +PRSDD+ WA+KT
Sbjct:   19 RWGPQHAGARQLAKLYSPGKRLQEWICVILCLSLFIVNLSFLFLNFSTVHIYRIIFGIVTGIVTADFASGMVHWGADTWGSVEIPVIGKAFIRPFREHHIDPTAITRHDFIETNGDNCMITILPLAHMAYKFLSYPPDALAES--YPWECFVFALAVFVTMTNQIHKWAHSYFDLPRWVTLLQDCHLVLPRKHHRIHHVSPHETYYCITTGWLNYPLDRVGFWRTMEWLIERLTGQRPRSDDLAWAKKT 265
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BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Zebrafish
Match: si:ch211-212o1.2 (si:ch211-212o1.2 [Source:ZFIN;Acc:ZDB-GENE-041111-310])

HSP 1 Score: 306.22 bits (783), Expect = 1.542e-100
Identity = 143/249 (57.43%), Postives = 180/249 (72.29%), Query Frame = 1 
Query:  271 KFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQKT 1017
            ++G QHAGA++LA  YS GKR QE +C ++   L + NL  L +NF       I+     GI+ AD  SG+VHW  DTWGSVE PVIG   IRPFREHHIDPT+ITRHDFIETNGDN    I   A++A+K   Y  + L     Y +  F++ L+VFV+ TNQ HKW+H+Y+ LPRWVT LQD H++LPRKHHR+HHVSPHETYYCITTGWLN+PL+++ FWR +E+LIE  TG +PRSDD+ WA+KT
Sbjct:   77 RWGPQHAGARQLAKLYSPGKRLQEWICVILCLSLFIVNLSFLFLNFSTVHIYRIIFGIVTGIVTADFASGMVHWGADTWGSVEIPVIGKAFIRPFREHHIDPTAITRHDFIETNGDNCMITILPLAHMAYKFLSYPPDALAES--YPWECFVFALAVFVTMTNQIHKWAHSYFDLPRWVTLLQDCHLVLPRKHHRIHHVSPHETYYCITTGWLNYPLDRVGFWRTMEWLIERLTGQRPRSDDLAWAKKT 323
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Zebrafish
Match: si:ch211-212o1.2 (si:ch211-212o1.2 [Source:ZFIN;Acc:ZDB-GENE-041111-310])

HSP 1 Score: 303.523 bits (776), Expect = 1.899e-100
Identity = 143/249 (57.43%), Postives = 180/249 (72.29%), Query Frame = 1 
Query:  271 KFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQKT 1017
            ++G QHAGA++LA  YS GKR QE +C ++   L + NL  L +NF       I+     GI+ AD  SG+VHW  DTWGSVE PVIG   IRPFREHHIDPT+ITRHDFIETNGDN    I   A++A+K   Y  + L     Y +  F++ L+VFV+ TNQ HKW+H+Y+ LPRWVT LQD H++LPRKHHR+HHVSPHETYYCITTGWLN+PL+++ FWR +E+LIE  TG +PRSDD+ WA+KT
Sbjct:   12 RWGPQHAGARQLAKLYSPGKRLQEWICVILCLSLFIVNLSFLFLNFSTVHIYRIIFGIVTGIVTADFASGMVHWGADTWGSVEIPVIGKAFIRPFREHHIDPTAITRHDFIETNGDNCMITILPLAHMAYKFLSYPPDALAES--YPWECFVFALAVFVTMTNQIHKWAHSYFDLPRWVTLLQDCHLVLPRKHHRIHHVSPHETYYCITTGWLNYPLDRVGFWRTMEWLIERLTGQRPRSDDLAWAKKT 258
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Zebrafish
Match: si:ch211-212o1.2 (si:ch211-212o1.2 [Source:ZFIN;Acc:ZDB-GENE-041111-310])

HSP 1 Score: 303.908 bits (777), Expect = 2.021e-100
Identity = 143/249 (57.43%), Postives = 180/249 (72.29%), Query Frame = 1 
Query:  271 KFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQKT 1017
            ++G QHAGA++LA  YS GKR QE +C ++   L + NL  L +NF       I+     GI+ AD  SG+VHW  DTWGSVE PVIG   IRPFREHHIDPT+ITRHDFIETNGDN    I   A++A+K   Y  + L     Y +  F++ L+VFV+ TNQ HKW+H+Y+ LPRWVT LQD H++LPRKHHR+HHVSPHETYYCITTGWLN+PL+++ FWR +E+LIE  TG +PRSDD+ WA+KT
Sbjct:   18 RWGPQHAGARQLAKLYSPGKRLQEWICVILCLSLFIVNLSFLFLNFSTVHIYRIIFGIVTGIVTADFASGMVHWGADTWGSVEIPVIGKAFIRPFREHHIDPTAITRHDFIETNGDNCMITILPLAHMAYKFLSYPPDALAES--YPWECFVFALAVFVTMTNQIHKWAHSYFDLPRWVTLLQDCHLVLPRKHHRIHHVSPHETYYCITTGWLNYPLDRVGFWRTMEWLIERLTGQRPRSDDLAWAKKT 264
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Xenopus
Match: cpne7 (copine VII [Source:Xenbase;Acc:XB-GENE-983652])

HSP 1 Score: 316.62 bits (810), Expect = 7.746e-105
Identity = 152/248 (61.29%), Postives = 180/248 (72.58%), Query Frame = 1 
Query:  271 KFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQK 1014
            ++G QHAGA+ELA  YS GKRFQE +C ++  IL+ +N + LI  F      SI    F GII AD  SGLVHW  DTWGSV+ P++G   IRPFREHHIDPT+ITRHDFIETNGDN  A +   A +A+K   Y  +L+     Y    FI+ L++FV+ TNQ HKWSHTY+GLP WV FLQDWHIILPRKHHR+HHVSPHETY+CITTGWLN+PLE I FWR LE  I+  TG KPRSDD+ WAQK
Sbjct:   42 RWGPQHAGARELAELYSSGKRFQEWICVILCFILLSFNFVCLIRYFSFEHTASIFVSIFAGIITADFASGLVHWGADTWGSVDLPIVGKAFIRPFREHHIDPTAITRHDFIETNGDNCMATLIPLACMAYKFLFYTPDLIY--KTYQLECFIFALAMFVTLTNQIHKWSHTYFGLPVWVVFLQDWHIILPRKHHRIHHVSPHETYFCITTGWLNYPLEMIGFWRRLEDFIQGVTGEKPRSDDLKWAQK 287
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Mouse
Match: Tmem189 (transmembrane protein 189 [Source:MGI Symbol;Acc:MGI:2142624])

HSP 1 Score: 303.523 bits (776), Expect = 3.354e-100
Identity = 144/259 (55.60%), Postives = 184/259 (71.04%), Query Frame = 1 
Query:  238 DINKDNVERIYKFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQK 1014
            ++++D      ++G+QHAGA+ELAA YS GKRFQE    ++   L+ +NL+ L++         ++     G ++AD LSGLVHW  DTWGSV+ P++G   IRPFREHHIDPT+ITRHDFIETNGDN    +     +A+K R    E L  +  Y +  F++ L++F +FTNQ HKWSHTY GLP WVT LQDWH+ILPRKHHR+HHV+PHETY+CITTGWLN+PLE I FWR LE LI+  TG KPR+DDM WAQK
Sbjct:   13 ELDEDETAEGRRWGAQHAGARELAALYSPGKRFQEWCSVILCFSLIAHNLVHLLLLARWEHTPLVILGVVAGALVADFLSGLVHWGADTWGSVDLPIVGKAFIRPFREHHIDPTAITRHDFIETNGDNCLVTLLPLLNMAYKFRTQSPETL--EQLYPWECFVFCLTIFGTFTNQIHKWSHTYLGLPYWVTVLQDWHVILPRKHHRIHHVAPHETYFCITTGWLNYPLEVIGFWRRLEDLIQGLTGEKPRADDMKWAQK 269
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Mouse
Match: Gm20431 (predicted gene 20431 [Source:MGI Symbol;Acc:MGI:5141896])

HSP 1 Score: 253.062 bits (645), Expect = 3.745e-79
Identity = 124/246 (50.41%), Postives = 165/246 (67.07%), Query Frame = 1 
Query:  238 DINKDNVERIYKFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATG 975
            ++++D      ++G+QHAGA+ELAA YS GKRFQE    ++   L+ +NL+ L++         ++     G ++AD LSGLVHW  DTWGSV+ P++G   IRPFREHHIDPT+ITRHDFIETNGDN    +     +A+K R    E L  +  Y +  F++ L++F +FTNQ HKWSHTY GLP WVT LQDWH+ILPRKHHR+HHV+PHETY+CITTG     ++  + +R LE L E   G
Sbjct:   13 ELDEDETAEGRRWGAQHAGARELAALYSPGKRFQEWCSVILCFSLIAHNLVHLLLLARWEHTPLVILGVVAGALVADFLSGLVHWGADTWGSVDLPIVGKAFIRPFREHHIDPTAITRHDFIETNGDNCLVTLLPLLNMAYKFRTQSPETL--EQLYPWECFVFCLTIFGTFTNQIHKWSHTYLGLPYWVTVLQDWHVILPRKHHRIHHVAPHETYFCITTG-----VKVPRNFRLLEELEEGQKG 251
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. UniProt/SwissProt
Match: sp|A6QLM0|TM189_BOVIN (Plasmanylethanolamine desaturase OS=Bos taurus OX=9913 GN=TMEM189 PE=2 SV=1)

HSP 1 Score: 310.071 bits (793), Expect = 6.432e-102
Identity = 146/259 (56.37%), Postives = 185/259 (71.43%), Query Frame = 1 
Query:  238 DINKDNVERIYKFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQK 1014
            ++  D      ++G+QHAGA+ELAA YS GKRFQE  C V+   L+ +N+  L++         ++     G +LAD LSGLVHW  DTWGSVE P++G   IRPFREHHIDPT+ITRHDFIETNGDN    +     +A+K R    E+L  +  Y +  F++ L +F +FTNQ HKWSHTY+GLP WV FLQDWH+ILPRKHHR+HHVSPHETY+CITTGWLN+PLE++ FWR LE +I++ TG KPR+DDM WAQK
Sbjct:   13 ELEDDEAASCRRWGAQHAGARELAALYSPGKRFQEWCCVVLCFSLIAHNMAHLLLLARWEHTPLVMLGMVAGALLADFLSGLVHWGADTWGSVELPIVGKAFIRPFREHHIDPTAITRHDFIETNGDNCLLTLLPLLNMAYKFRTQSPEVL--EQLYPWECFVFCLIIFGTFTNQIHKWSHTYFGLPCWVVFLQDWHVILPRKHHRIHHVSPHETYFCITTGWLNYPLERMGFWRRLEDIIQALTGEKPRADDMKWAQK 269
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. UniProt/SwissProt
Match: sp|A5PLL7|TM189_HUMAN (Plasmanylethanolamine desaturase OS=Homo sapiens OX=9606 GN=TMEM189 PE=1 SV=3)

HSP 1 Score: 306.99 bits (785), Expect = 1.182e-100
Identity = 146/248 (58.87%), Postives = 181/248 (72.98%), Query Frame = 1 
Query:  271 KFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQK 1014
            ++G+QHAGA+ELAA YS GKR QE    ++   L+ +NL+ L++         ++     G ++AD LSGLVHW  DTWGSVE P++G   IRPFREHHIDPT+ITRHDFIETNGDN    +     +A+K R +  E L  +  Y +  F++ L +F +FTNQ HKWSHTY+GLPRWVT LQDWH+ILPRKHHR+HHVSPHETY+CITTGWLN+PLEKI FWR LE LI+  TG KPR+DDM WAQK
Sbjct:   23 RWGAQHAGARELAALYSPGKRLQEWCSVILCFSLIAHNLVHLLLLARWEDTPLVILGVVAGALIADFLSGLVHWGADTWGSVELPIVGKAFIRPFREHHIDPTAITRHDFIETNGDNCLVTLLPLLNMAYKFRTHSPEAL--EQLYPWECFVFCLIIFGTFTNQIHKWSHTYFGLPRWVTLLQDWHVILPRKHHRIHHVSPHETYFCITTGWLNYPLEKIGFWRRLEDLIQGLTGEKPRADDMKWAQK 268
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. UniProt/SwissProt
Match: sp|Q99LQ7|TM189_MOUSE (Plasmanylethanolamine desaturase OS=Mus musculus OX=10090 GN=Tmem189 PE=1 SV=1)

HSP 1 Score: 303.523 bits (776), Expect = 2.346e-99
Identity = 144/259 (55.60%), Postives = 184/259 (71.04%), Query Frame = 1 
Query:  238 DINKDNVERIYKFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQK 1014
            ++++D      ++G+QHAGA+ELAA YS GKRFQE    ++   L+ +NL+ L++         ++     G ++AD LSGLVHW  DTWGSV+ P++G   IRPFREHHIDPT+ITRHDFIETNGDN    +     +A+K R    E L  +  Y +  F++ L++F +FTNQ HKWSHTY GLP WVT LQDWH+ILPRKHHR+HHV+PHETY+CITTGWLN+PLE I FWR LE LI+  TG KPR+DDM WAQK
Sbjct:   13 ELDEDETAEGRRWGAQHAGARELAALYSPGKRFQEWCSVILCFSLIAHNLVHLLLLARWEHTPLVILGVVAGALVADFLSGLVHWGADTWGSVDLPIVGKAFIRPFREHHIDPTAITRHDFIETNGDNCLVTLLPLLNMAYKFRTQSPETL--EQLYPWECFVFCLTIFGTFTNQIHKWSHTYLGLPYWVTVLQDWHVILPRKHHRIHHVAPHETYFCITTGWLNYPLEVIGFWRRLEDLIQGLTGEKPRADDMKWAQK 269
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9AE87|CARF_MYXXA (Plasmanylethanolamine desaturase OS=Myxococcus xanthus OX=34 GN=carF PE=1 SV=1)

HSP 1 Score: 176.022 bits (445), Expect = 5.229e-50
Identity = 102/236 (43.22%), Postives = 128/236 (54.24%), Query Frame = 1 
Query:  295 AKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMN 1002
            A+ LA  YS   R  E    V +  L V  +  L        W  +L     G + AD +SG VHW  DTWGS E PV+G  LIRPFREHH+D  +ITRHDF+ETNG+N    +P  A +A  L       +    +    +F+  +  +V  TNQFHKWSH     P  V FLQ  H+ILP  HHR+HH  P+  YYCIT GW+N PL  + F+   E LI  ATG  PR DD+ 
Sbjct:   15 AQVLAQGYSPAIRAMEIAAIVSFVSLEVALVYRLWGTPYAGTWL-LLSAVLLGYLAADFVSGFVHWMGDTWGSTEMPVLGKALIRPFREHHVDEKAITRHDFVETNGNNCLISLP-VAIIALCLP------MSGPGWVFCASFLGAMIFWVMATNQFHKWSH-MDSPPALVGFLQRVHLILPPDHHRIHHTKPYNKYYCITVGWMNKPLTMVHFFPTAERLITWATGLLPRQDDIG 241
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9SZ42|FAD4_ARATH (Fatty acid desaturase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4 PE=1 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 3.295e-22
Identity = 74/230 (32.17%), Postives = 108/230 (46.96%), Query Frame = 1 
Query:  358 IWAILMVYNLILLIINFVPSQWKSILCY-----AFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSH-TYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRS---DDMNWAQKTH 1020
            +W       L + +   V   + S LC       + G ILADL SG+ HW  D +G    PV+G   I  F+ HH  P +ITR  F   N   LA VI FT             L +A N   F+ F+   +  + F+ QFH W+H T   LP  V  LQD  +++ R+ H  HH +P+   YCI +G  N  L++ + +  LE +     G +PRS    + +W ++T 
Sbjct:  100 LWVAAGCTTLFVSLAKSVIGGFDSHLCLEPALAGYAGYILADLGSGVYHWAIDNYGDESTPVVG-TQIEAFQGHHKWPWTITRRQF-ANNLHALAQVITFTVL----------PLDLAFNDPVFHGFVCTFAFCILFSQQFHAWAHGTKSKLPPLVVALQDMGLLVSRRQHAEHHRAPYNNNYCIVSGAWNNVLDESKVFEALEMVFYFQLGVRPRSWSEPNSDWIEETE 317
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. TrEMBL
Match: K1P739 (TMEM189_B_dmain domain-containing protein OS=Crassostrea gigas OX=29159 GN=CGI_10008502 PE=4 SV=1)

HSP 1 Score: 342.813 bits (878), Expect = 4.497e-112
Identity = 160/270 (59.26%), Postives = 198/270 (73.33%), Query Frame = 1 
Query:  205 GMPEISYQVSDDINKDNVERIYKFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQK 1014
             MPE     + D +     R   +G QHAGAKELA+ YS GKR QET+C ++   LM +N + L+++F    W +IL  +  GI++AD  SGLVHW  DTWGS+E PVIG   IRPFREHHIDPTSITRHDFIETNGDN A  +PF A++A+K      E +     Y++  F++LL++FVS TNQFHKWSHTY+GLP+W+TFLQD HIILP+KHHR+HHV+PHETY+CITTGWLN+PLEKI FW FLE +IE  TG+KPR DD  WAQK
Sbjct:   14 SMPEDDPNSNSDFSSAAKPR---WGPQHAGAKELASHYSSGKRLQETICLLVCITLMAFNFMQLVMHFKSENWYTILVCSLFGILVADFFSGLVHWGADTWGSIEIPVIGKAFIRPFREHHIDPTSITRHDFIETNGDNFAVTVPFLAHMAYKFVSLSPEEI--NKTYNWECFVFLLAIFVSLTNQFHKWSHTYFGLPKWITFLQDMHIILPKKHHRIHHVAPHETYFCITTGWLNYPLEKIGFWTFLEDVIERTTGNKPRDDDFKWAQK 278
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. TrEMBL
Match: A0A1S3H995 (transmembrane protein 189 OS=Lingula unguis OX=7574 GN=LOC106153330 PE=4 SV=1)

HSP 1 Score: 342.043 bits (876), Expect = 1.032e-111
Identity = 168/265 (63.40%), Postives = 194/265 (73.21%), Query Frame = 1 
Query:  232 SDDINKDNV----ERIYKFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQK 1014
             +D N +NV    E I ++G QH GA+ELA  YS  KR QETVC V+   LMV NL  LI+NF    W+ IL  A  G I AD  SGLVHW  DTWGSVE P+IG  LIRPFREHHIDPTSITRHDFIETNGDN A +IPF  ++A+K   Y  + +  Q  Y +  ++YLL+ +VS TNQ HKWSHTY+GLPR++T  QD HIILPRKHHR+HHVSPHETY+CITTGWLN+PLEKI FWR  E LIE  TGSKPRSDDM WAQK
Sbjct:   19 EEDPNYNNVRETTETIPRWGPQHKGAQELAGLYSSSKRTQETVCVVVCLGLMVLNLYHLIVNFQADNWQYILVAAVLGSITADFFSGLVHWGADTWGSVELPIIGKTLIRPFREHHIDPTSITRHDFIETNGDNFAVIIPFLGHMAFKFATYSPDQV--QATYIWECYVYLLAFYVSITNQIHKWSHTYFGLPRYITIFQDLHIILPRKHHRIHHVSPHETYFCITTGWLNYPLEKIGFWRRAEDLIEKLTGSKPRSDDMKWAQK 281
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. TrEMBL
Match: A0A210PT33 (Transmembrane protein 189 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT13546 PE=4 SV=1)

HSP 1 Score: 339.732 bits (870), Expect = 7.671e-111
Identity = 157/273 (57.51%), Postives = 201/273 (73.63%), Query Frame = 1 
Query:  217 ISYQVSDDINKDNVERIY-------KFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQK 1014
            I Y ++++    N + +        ++G QHAGA+ELA++Y+ GKR QET+C  +   L+ +N I+L  NF   +W  ++  A  GI+ AD +SGLVHW  D+WGSVE P+IG   IRPFREHHIDPTSITRHDFIETNGDN +  IPF AY A+K   Y  E +  Q  Y+F  +++LL++FVS TNQ HKWSHTY+GLPRW+T LQD HI+LPRKHHR+HHV+PHETY+CITTGWLN PLEKI+FW   E+LIE+ TG KPRSDD+NWA+K
Sbjct:   10 IKYSMTEEDPNSNAQIVIAEEVSKPRWGPQHAGAQELASQYTKGKRIQETICIGVSTTLIAHNFIMLCWNFHLDKWYFVIASALLGILSADFMSGLVHWTADSWGSVELPIIGKAFIRPFREHHIDPTSITRHDFIETNGDNFSVTIPFMAYSAYKFLTYSPEQI--QEVYNFECYLFLLTIFVSVTNQCHKWSHTYFGLPRWITLLQDLHIVLPRKHHRIHHVAPHETYFCITTGWLNLPLEKIRFWSTFEYLIEAVTGCKPRSDDLNWAKK 280
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. TrEMBL
Match: V5H9R1 (Putative ubiquitin-conjugating enzyme protein (Fragment) OS=Ixodes ricinus OX=34613 PE=2 SV=1)

HSP 1 Score: 336.65 bits (862), Expect = 1.979e-109
Identity = 160/276 (57.97%), Postives = 200/276 (72.46%), Query Frame = 1 
Query:  199 EIGMPEISYQVSDDINKDNV----ERIYKFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQK 1014
            E  MPE     + D  + N     E   ++G  HAGA+ELA+ Y+ GKR QETVC  +   LMVYNL  L+  F  S+W SI+  A CG++ AD LSGLVHW  DTWGSVE P+IG   IRPFREHHIDPTSITRHDFIETNGDN    IP  A +AW    +  + ++A+  Y +  F++LL++FV+FTNQ HKWSHTY+GLPRWVT LQ WH++LPR+HHR+HHV+PHETY+CITTGWLN+PLEK++FW  LE +I + TG +PRSDD+ WAQK
Sbjct:   18 ENSMPEDDPNSNSDTYQVNEQFQGELKPRWGPYHAGARELASLYTRGKRTQETVCIGVCVTLMVYNLCQLLYYFQASRWSSIVAVALCGVVTADFLSGLVHWAADTWGSVELPIIGKAFIRPFREHHIDPTSITRHDFIETNGDNFMVPIPVLAAMAWGFASWRPQDILAR--YHWNCFLFLLALFVAFTNQIHKWSHTYFGLPRWVTLLQAWHVVLPRQHHRIHHVAPHETYFCITTGWLNYPLEKLRFWAALEGVISALTGCRPRSDDLRWAQK 291
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. TrEMBL
Match: A0A224ZBF3 (Ubiquitin conjugating enzyme OS=Rhipicephalus zambeziensis OX=60191 PE=4 SV=1)

HSP 1 Score: 333.954 bits (855), Expect = 2.086e-108
Identity = 157/276 (56.88%), Postives = 196/276 (71.01%), Query Frame = 1 
Query:  199 EIGMPEISYQVSDDI----NKDNVERIYKFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQK 1014
            E  MPE     + D     N  + +   ++G  HAGA+ELA  Y+ GKR QETVC  +   LM YNL  L++ F  S+W +I+  A CG++ AD LSGLVHW  DTWGSVE PVIG   IRPFREHHIDPTSITRHDFIETNGDN    IP  A +AW       + ++A   Y +  F++LL++FV+FTNQ HKWSHTY+GLPRWVT LQ+WH++LPR+HHR+HHV+PHETY+CITTGWLN+PLEK+ FW  LE +I + TG +PRSDD+ WA K
Sbjct:   19 ENSMPEDDPNSNSDTYQPSNAVDGQLKPRWGPHHAGARELAGLYTRGKRTQETVCIAVCLTLMGYNLFQLMLYFQASRWSTIIAAALCGVVTADFLSGLVHWAADTWGSVELPVIGKAFIRPFREHHIDPTSITRHDFIETNGDNFMVPIPVLASMAWGFASKPPQAILAT--YHWNCFLFLLALFVAFTNQIHKWSHTYFGLPRWVTLLQEWHVVLPRQHHRIHHVAPHETYFCITTGWLNYPLEKLHFWPALEAVISALTGCRPRSDDLQWALK 292
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Cavefish
Match: si:ch211-212o1.2 (transmembrane protein 189 [Source:NCBI gene;Acc:103038667])

HSP 1 Score: 315.464 bits (807), Expect = 8.567e-105
Identity = 149/249 (59.84%), Postives = 182/249 (73.09%), Query Frame = 1 
Query:  271 KFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQKT 1017
            ++G QHAGA++LA  YS GKR QE +C ++   L   N ILL+INF       I+     GI+ AD  SG+VHW  DTWGSV+ PVIG   IRPFREHHIDPT+ITRHDFIETNGDN    I   A++A+KL  Y  + L     Y +  F++ L+VFV+ TNQ HKWSH+Y+GLPRWVT LQD H++LPRKHHR+HHVSPHETYYCITTGWLN+PL++I FWR +E+LIE  TG KPR DDM WA+KT
Sbjct:   36 RWGPQHAGARQLAKLYSPGKRLQEWICVILCLSLFAVNFILLLINFSTVHIYRIIFGIVTGIVTADFASGMVHWGADTWGSVDIPVIGKAFIRPFREHHIDPTAITRHDFIETNGDNCMITILPLAHMAYKLMSYTPDALAES--YPWECFVFALAVFVTMTNQIHKWSHSYFGLPRWVTLLQDCHLVLPRKHHRIHHVSPHETYYCITTGWLNYPLDRIGFWRRMEWLIERVTGQKPRCDDMAWAKKT 282
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Cavefish
Match: tmem189 (transmembrane protein 189-like [Source:NCBI gene;Acc:103028470])

HSP 1 Score: 295.434 bits (755), Expect = 5.477e-97
Identity = 147/251 (58.57%), Postives = 178/251 (70.92%), Query Frame = 1 
Query:  271 KFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNY--YSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQKT 1017
            ++G QHAGA+ELA  YS GKR QE +  V+   LM +N   L+ +F       I+     GI+ AD  SGLVHW  DTWGSV+ P+ G   IRPFREHHIDPT+IT+HDFIETNGDN    I   A +A+     +  L   + Y  Y  Y ++Y L++FV+ TNQ HKWSHTY+GLPRWVT LQD HIILPRKHHR+HHVSPHETY+CITTGWLN+PLEK+ FWR LE +I+S TG KPRSDD+ WAQKT
Sbjct:   28 RWGPQHAGAQELAQLYSPGKRCQEWISVVLCFALMAFNFFHLLAHFHLGHVWYIVLGIVAGILTADFASGLVHWGADTWGSVDLPIFGKAFIRPFREHHIDPTAITQHDFIETNGDNCMLTIVPLANMAFN----FLTLTPGEIYHTYPLYCYLYALAIFVTLTNQIHKWSHTYFGLPRWVTLLQDCHIILPRKHHRVHHVSPHETYFCITTGWLNYPLEKMGFWRNLEDVIQSLTGEKPRSDDLKWAQKT 274
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Nematostella
Match: EDO41601 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3V1])

HSP 1 Score: 241.891 bits (616), Expect = 6.495e-78
Identity = 110/188 (58.51%), Postives = 138/188 (73.40%), Query Frame = 1 
Query:  451 GIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQK 1014
            G+++AD +SGLVHW  DTWGSV+ P+ G   IRPFREHH+DPT+ITRHD IETNGDN    +P  + +  K   +  +     ++Y+   F++ L  FV  TNQ HKWSHTY+GLP WV  LQ WHIILP+ HHR+HHV+PHETY+CITTGWL++PLE I+FW  LE+LIE  TG KPR+DDM WA K
Sbjct:    4 GMVVADFMSGLVHWAADTWGSVDIPIFGKAFIRPFREHHVDPTAITRHDLIETNGDNCLLTLPVLSVVTAKY--FICDQNTISSWYALDCFLFSLGFFVMLTNQIHKWSHTYFGLPWWVEKLQSWHIILPKLHHRIHHVAPHETYFCITTGWLDYPLELIRFWPALEYLIEKITGHKPRADDMTWASK 189
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Nematostella
Match: EDO41602 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3V2])

HSP 1 Score: 238.81 bits (608), Expect = 8.623e-77
Identity = 109/187 (58.29%), Postives = 137/187 (73.26%), Query Frame = 1 
Query:  454 IILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQK 1014
            +++AD +SGLVHW  DTWGSV+ P+ G   IRPFREHH+DPT+ITRHD IETNGDN    +P  + +  K   +  +     ++Y+   F++ L  FV  TNQ HKWSHTY+GLP WV  LQ WHIILP+ HHR+HHV+PHETY+CITTGWL++PLE I+FW  LE+LIE  TG KPR+DDM WA K
Sbjct:    1 MVVADFMSGLVHWAADTWGSVDIPIFGKAFIRPFREHHVDPTAITRHDLIETNGDNCLLTLPVLSVVTAKY--FICDQNTISSWYALDCFLFSLGFFVMLTNQIHKWSHTYFGLPWWVEKLQSWHIILPKLHHRIHHVAPHETYFCITTGWLDYPLELIRFWPALEYLIEKITGHKPRADDMTWASK 185
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Medaka
Match: si:ch211-212o1.2 (transmembrane protein 189 [Source:NCBI gene;Acc:101156405])

HSP 1 Score: 305.064 bits (780), Expect = 1.058e-100
Identity = 143/249 (57.43%), Postives = 180/249 (72.29%), Query Frame = 1 
Query:  271 KFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQKT 1017
            ++G QHAGA++LA  YS GKR QE VC ++  +L + N   L+++F       I+     GI+ AD  SG+VHW  DTWGSV+ PVIG   IRPFREHHIDPT+ITRHDFIETNGDN    I   A++A+K      E L     + +  +++ L+VFV+ TNQ HKWSH+Y+GLPRWVT LQDWH++LPRKHHR+HHVSPHETYYCITTGW N+PL+++ FWR +E LIE  TG KPRSDDM WA+KT
Sbjct:   36 RWGPQHAGARQLARLYSPGKRLQEWVCVILCLLLFIVNFSFLLLHFSTVHIYKIVLSIVLGIVTADFASGVVHWGADTWGSVDIPVIGRAFIRPFREHHIDPTAITRHDFIETNGDNCMIPILPLAHMAYKFLTCTPETLAVS--FPWNCYVFALAVFVTLTNQIHKWSHSYFGLPRWVTLLQDWHLVLPRKHHRIHHVSPHETYYCITTGWCNYPLDQLGFWRRMERLIERVTGQKPRSDDMAWAKKT 282
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Medaka
Match: tmem189 (transmembrane protein 189 [Source:ZFIN;Acc:ZDB-GENE-041010-150])

HSP 1 Score: 298.13 bits (762), Expect = 4.678e-98
Identity = 146/251 (58.17%), Postives = 180/251 (71.71%), Query Frame = 1 
Query:  271 KFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNY--YSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQKT 1017
            ++G QHAGA+ELA  YS GKR QE +  V+   LM +N I L+ NF      SIL     GI+ AD  SG+VHW  DTWGSV+ P+ G   IRPFREHHIDPT+ITRHDFIETNGDN    +   A +A+     +  L  A+ Y  Y +Y +++ L++FV+ TNQ HKWSHTY+GLPRWV FLQD  IILPRKHHR+HHVSPHETY+CITTGWLN+PLEK+ FWR+LE LI+  TG KPR+DD+ WA+K 
Sbjct:   28 RWGPQHAGAQELANLYSPGKRLQEWISVVLCFSLMAFNFIHLLANFHLGHSWSILLAIVAGILTADFASGMVHWGADTWGSVDLPIFGKAFIRPFREHHIDPTAITRHDFIETNGDNCMLTLLPLANMAFN----FLTLSPAEIYHNYPWYCYLFALAIFVTLTNQIHKWSHTYFGLPRWVVFLQDCQIILPRKHHRIHHVSPHETYFCITTGWLNYPLEKLGFWRYLEDLIQGVTGEKPRADDLKWAKKV 274
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Planmine SMEST
Match: SMESG000012315.1 (SMESG000012315.1)

HSP 1 Score: 585.874 bits (1509), Expect = 0.000e+0
Identity = 280/281 (99.64%), Postives = 281/281 (100.00%), Query Frame = 1 
Query:  178 MSLLEGKEIGMPEISYQVSDDINKDNVERIYKFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQKTH 1020
            MSLLEGKEIGMPEISYQVSDDINKDNVERIYKFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLI+SATGSKPRSDDMNWAQKTH
Sbjct:    1 MSLLEGKEIGMPEISYQVSDDINKDNVERIYKFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIKSATGSKPRSDDMNWAQKTH 281
[Close]
BLAST of TMEM189_B_dmain domain-containing protein vs. Planmine SMEST
Match: SMESG000012315.1 (SMESG000012315.1)

HSP 1 Score: 564.688 bits (1454), Expect = 0.000e+0
Identity = 270/271 (99.63%), Postives = 271/271 (100.00%), Query Frame = 1 
Query:  208 MPEISYQVSDDINKDNVERIYKFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIESATGSKPRSDDMNWAQKTH 1020
            MPEISYQVSDDINKDNVERIYKFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLI+SATGSKPRSDDMNWAQKTH
Sbjct:    1 MPEISYQVSDDINKDNVERIYKFGSQHAGAKELAAKYSFGKRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLSGLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLAAVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWSHTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEKIQFWRFLEFLIKSATGSKPRSDDMNWAQKTH 271
The following BLAST results are available for this feature:
BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
TMEM1892.461e-10158.87transmembrane protein 189 [Source:HGNC Symbol;Acc:... [more]
TMEM1895.924e-9858.47transmembrane protein 189 [Source:HGNC Symbol;Acc:... [more]
TMEM1891.864e-8965.97transmembrane protein 189 [Source:HGNC Symbol;Acc:... [more]
TMEM189-UBE2V12.972e-7953.19TMEM189-UBE2V1 readthrough [Source:HGNC Symbol;Acc... [more]
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BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Y53C10A.54.961e-8547.84pep chromosome:WBcel235:I:11998781:12002643:1 gene... [more]
Y53C10A.54.961e-8547.84pep chromosome:WBcel235:I:11998781:12002612:1 gene... [more]
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BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Kua2.086e-9857.26gene:FBgn0032850 transcript:FBtr0331617[more]
Kua2.086e-9857.26gene:FBgn0032850 transcript:FBtr0331616[more]
Kua2.086e-9857.26gene:FBgn0032850 transcript:FBtr0081336[more]
Kua2.086e-9857.26gene:FBgn0032850 transcript:FBtr0303230[more]
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BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
si:ch211-212o1.21.080e-10057.43si:ch211-212o1.2 [Source:ZFIN;Acc:ZDB-GENE-041111-... [more]
si:ch211-212o1.21.313e-10057.43si:ch211-212o1.2 [Source:ZFIN;Acc:ZDB-GENE-041111-... [more]
si:ch211-212o1.21.542e-10057.43si:ch211-212o1.2 [Source:ZFIN;Acc:ZDB-GENE-041111-... [more]
si:ch211-212o1.21.899e-10057.43si:ch211-212o1.2 [Source:ZFIN;Acc:ZDB-GENE-041111-... [more]
si:ch211-212o1.22.021e-10057.43si:ch211-212o1.2 [Source:ZFIN;Acc:ZDB-GENE-041111-... [more]
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BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
cpne77.746e-10561.29copine VII [Source:Xenbase;Acc:XB-GENE-983652][more]
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BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Tmem1893.354e-10055.60transmembrane protein 189 [Source:MGI Symbol;Acc:M... [more]
Gm204313.745e-7950.41predicted gene 20431 [Source:MGI Symbol;Acc:MGI:51... [more]
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BLAST of TMEM189_B_dmain domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|A6QLM0|TM189_BOVIN6.432e-10256.37Plasmanylethanolamine desaturase OS=Bos taurus OX=... [more]
sp|A5PLL7|TM189_HUMAN1.182e-10058.87Plasmanylethanolamine desaturase OS=Homo sapiens O... [more]
sp|Q99LQ7|TM189_MOUSE2.346e-9955.60Plasmanylethanolamine desaturase OS=Mus musculus O... [more]
sp|Q9AE87|CARF_MYXXA5.229e-5043.22Plasmanylethanolamine desaturase OS=Myxococcus xan... [more]
sp|Q9SZ42|FAD4_ARATH3.295e-2232.17Fatty acid desaturase 4, chloroplastic OS=Arabidop... [more]
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BLAST of TMEM189_B_dmain domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
K1P7394.497e-11259.26TMEM189_B_dmain domain-containing protein OS=Crass... [more]
A0A1S3H9951.032e-11163.40transmembrane protein 189 OS=Lingula unguis OX=757... [more]
A0A210PT337.671e-11157.51Transmembrane protein 189 OS=Mizuhopecten yessoens... [more]
V5H9R11.979e-10957.97Putative ubiquitin-conjugating enzyme protein (Fra... [more]
A0A224ZBF32.086e-10856.88Ubiquitin conjugating enzyme OS=Rhipicephalus zamb... [more]
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BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
si:ch211-212o1.28.567e-10559.84transmembrane protein 189 [Source:NCBI gene;Acc:10... [more]
tmem1895.477e-9758.57transmembrane protein 189-like [Source:NCBI gene;A... [more]
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BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO416016.495e-7858.51Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO416028.623e-7758.29Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of TMEM189_B_dmain domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
si:ch211-212o1.21.058e-10057.43transmembrane protein 189 [Source:NCBI gene;Acc:10... [more]
tmem1894.678e-9858.17transmembrane protein 189 [Source:ZFIN;Acc:ZDB-GEN... [more]
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BLAST of TMEM189_B_dmain domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000012315.10.000e+099.64SMESG000012315.1[more]
SMESG000012315.10.000e+099.63SMESG000012315.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031558 ID=SMED30031558|Name=TMEM189_B_dmain domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1524bp
TGGTTATTTAACATTGTCTATTAAAGTTTTAATTTAAGTTAATCCAATCA
ACCGAATTATTATTTTTTATAGAATTTTCTAAGTTTTCTTGTTTGCTAGT
CCTTGATTTTATAATTATTTTATTGCTAAATAAAATCCAAAGATATTTAA
CTTCGTGTATTATTACAGAAGAAAATTATGTCATTATTAGAAGGTAAAGA
AATAGGAATGCCTGAGATCAGTTATCAAGTTTCAGATGATATTAATAAGG
ACAATGTAGAAAGAATTTATAAATTCGGATCACAGCACGCTGGTGCTAAA
GAGCTAGCTGCTAAATATAGTTTTGGCAAACGATTCCAGGAAACGGTTTG
TTGTGTAATCTGGGCAATTTTAATGGTTTACAATCTTATTTTACTTATAA
TTAATTTTGTGCCAAGCCAGTGGAAATCAATATTATGTTATGCATTTTGT
GGTATTATTTTGGCAGATTTATTATCAGGATTAGTTCATTGGTTTTGTGA
CACTTGGGGTTCGGTGGAATTTCCAGTTATCGGTCCGGGTTTAATTCGTC
CATTCCGTGAACACCATATTGATCCAACATCAATTACAAGGCATGATTTT
ATTGAAACAAACGGGGATAATTTGGCTGCAGTGATTCCATTTACTGCTTA
TTTGGCTTGGAAATTGAGAAAATACTATTCAGAGCTTCTAGTCGCACAGA
ATTATTATTCATTCTATGCCTTCATATATTTGTTGTCGGTGTTTGTTAGC
TTCACGAATCAGTTCCACAAATGGTCTCATACATATTATGGGCTCCCTCG
ATGGGTGACATTTTTGCAGGATTGGCACATAATATTGCCGCGAAAGCACC
ATCGGCTCCATCACGTATCTCCTCACGAAACATACTATTGCATCACTACC
GGTTGGCTGAATTGGCCTTTAGAAAAGATCCAATTCTGGCGATTTCTTGA
GTTCCTCATAGAGTCAGCAACTGGATCAAAACCCAGATCAGATGACATGA
ATTGGGCACAGAAAACTCATTAAATTGATTATAAAATTTCATCGAATTAT
TATTATTTTTTAAATTATCTATTTACGTTTTGGCGGTTTCTTGAATCGTT
GCAAATATATGGAATATTATTAATAGATGATGCTATTTTGTTTATATTTG
TGTGAGTGCGCGTGTGCGAGTTTTATACTGTGCTTTGGTGATCGATATAT
TCTGCGATTTGTTTGCACGCTGGCAAATTGTTTCGGTGCTGCTAATACTG
CCATTGTCATATGGTGATTCTCCAGCATCTCATTTATTCACCATGGAGGA
TCAGTGAGGATCAGTGAGATTTATGCACAGTTTAATAAATTTGAGTTCTT
TCATTGATTTTTGTATTAATTGATATTATTGATTTGTTTATGTAGGTGCT
CGTCATTCGATTGCGCTATTATGACAGGGAACAGTCAACATCAACTGCTT
CCATGTGCTTTAATTTTAGTTGCGTTTACCATGACTACTAGATAAAAGTT
CTAATTATTAAGATAAATTTAAAC
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protein sequence of SMED30031558-orf-1

>SMED30031558-orf-1 ID=SMED30031558-orf-1|Name=SMED30031558-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=282bp
MSLLEGKEIGMPEISYQVSDDINKDNVERIYKFGSQHAGAKELAAKYSFG
KRFQETVCCVIWAILMVYNLILLIINFVPSQWKSILCYAFCGIILADLLS
GLVHWFCDTWGSVEFPVIGPGLIRPFREHHIDPTSITRHDFIETNGDNLA
AVIPFTAYLAWKLRKYYSELLVAQNYYSFYAFIYLLSVFVSFTNQFHKWS
HTYYGLPRWVTFLQDWHIILPRKHHRLHHVSPHETYYCITTGWLNWPLEK
IQFWRFLEFLIESATGSKPRSDDMNWAQKTH*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0002075pigment cell
Vocabulary: INTERPRO
TermDefinition
IPR019547TMEM189_B_dmain
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
GO:0005783endoplasmic reticulum
GO:0005789endoplasmic reticulum membrane
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: biological process
TermDefinition
GO:0016567protein ubiquitination
Vocabulary: molecular function
TermDefinition
GO:0031625ubiquitin protein ligase binding
GO:0061630ubiquitin protein ligase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019547B domain of TMEM189, localisation domainPFAMPF10520TMEM189_B_dmaincoord: 93..272
e-value: 2.2E-73
score: 246.1
NoneNo IPR availableTMHMMTMhelixcoord: 85..107
NoneNo IPR availableTMHMMTMhelixcoord: 56..78
NoneNo IPR availableTMHMMTMhelixcoord: 170..192