SMED30031552

Overview
NameSMED30031552
Smed IDSMED30031552
Length (bp)3813
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30031552 (SMED30031552) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30031552 (SMED30031552) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30031552 (SMED30031552) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30031552 (SMED30031552) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30031552

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 63

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30031552h1SMcG0008966 dd_Smed_v4_1574_6_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30031552h1SMcG0008966 dd_Smed_v4_1574_6_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30031552h1SMcG0008966 dd_Smed_v4_1574_6_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30031552h1SMcG0008966 dd_Smed_v4_1574_6_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30031552h1SMcG0008966 dd_Smed_v4_1574_6_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
muscle cellSMED30031552h1SMcG0008966 dd_Smed_v4_1574_6_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30031552h1SMcG0008966 dd_Smed_v4_1574_6_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031552h1SMcG0008966 dd_Smed_v4_1574_6_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30031552h1SMcG0008966 dd_Smed_v4_1574_6_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30031552h1SMcG0008966 dd_Smed_v4_1574_6_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30031552h1SMcG0000098 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30031552h1SMcG0000098 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30031552h1SMcG0000098 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031552h1SMcG0000098 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30031552h1SMcG0000090 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30031552h1SMcG0000090 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30031552h1SMcG0000090 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031552h1SMcG0000090 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30031552h1SMcG0009086 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30031552h1SMcG0009086 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30031552h1SMcG0009086 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031552h1SMcG0009086 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30031552h1SMcG0008990 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30031552h1SMcG0008990 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30031552h1SMcG0008990 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031552h1SMcG0008990 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30031552h1SMcG0008966 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30031552h1SMcG0008966 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30031552h1SMcG0008966 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031552h1SMcG0008966 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30031552h1SMcG0009107 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30031552h1SMcG0009107 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30031552h1SMcG0009107 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031552h1SMcG0009107 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30031552h1SMcG0009109 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30031552h1SMcG0009109 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30031552h1SMcG0009109 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031552h1SMcG0009109 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30031552h1SMcG0009106 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30031552h1SMcG0009106 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30031552h1SMcG0009106 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031552h1SMcG0009106 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30031552h1SMcG0008970 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30031552h1SMcG0008970 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30031552h1SMcG0008970 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031552h1SMcG0008970 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30031552h1SMcG0009120 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30031552h1SMcG0009120 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30031552h1SMcG0009120 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031552h1SMcG0009120 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30031552h1SMcG0009147 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30031552h1SMcG0009147 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30031552h1SMcG0009147 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031552h1SMcG0009147 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30031552h1SMcG0009192 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30031552h1SMcG0009192 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30031552h1SMcG0009192 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031552h1SMcG0009192 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30031552h1SMcG0009184 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30031552h1SMcG0009184 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30031552h1SMcG0009184 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031552h1SMcG0009184 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30031552h1SMcG0008966 dd_Smed_v4_1574_6_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30031552 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000049:5354..8557 +14878
v31.004077:22830..26031 +11599
Homology
[Close]
BLAST of SMED30031552 vs. UniProt/SwissProt
Match: sp|Q8JHV9|BIR7A_XENLA (Baculoviral IAP repeat-containing protein 7-A OS=Xenopus laevis OX=8355 GN=birc7-a PE=1 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 1.563e-6
Identity = 33/113 (29.20%), Postives = 55/113 (48.67%), Query Frame = -1 
Query:  118 MLLSGVKTNTMRKIISERLANNLEWFDSTQ-AVIRAIKRYQ--EKPKES---LEKSMIKKAILEQHQCVTCWQKHAEIIYKPCGHMVNSEACGLNVNLCPVCRQQINGRIKGF 438
             LL+G   +++  ++++ L    E   +   +V RA  R +  E PKES   L      + + E+  C  C  K   +++ PCGH+V    C  N+  CP+CR  I G ++ F
Sbjct:  287 FLLTGSSYSSVSDLVTDLLVAEEETHSTESVSVSRAPTRMERSEPPKESAPPLSTEEQLRRLKEERMCKVCMDKDVSMLFVPCGHLVVCTECAPNLRHCPICRAAIRGSVRAF 399
[Close]
BLAST of SMED30031552 vs. TrEMBL
Match: A0A218USP6 (Baculoviral IAP repeat-containing protein 7-B OS=Lonchura striata domestica OX=299123 GN=BIRC7-B PE=4 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 3.656e-6
Identity = 33/107 (30.84%), Postives = 58/107 (54.21%), Query Frame = -1 
Query:  118 MLLSGVKTNTMRKIISERLANNLEWFDSTQAVIRAIKRYQEKPKESLEKSMIKKAILEQHQCVTCWQKHAEIIYKPCGHMVNSEACGLNVNLCPVCRQQINGRIKGF 438
             +L+G    +  ++IS+ L +   W  S+ A  R     +++ + S E+ +  + + E+  C  C  K   +++ PCGH+V  E C LN+ LCP+CR  I GR++ F
Sbjct:  214 FVLTGTFYLSESELISDLLQSG--WGASSSAGGR-----RDESQLSTEEQL--RRLQEERTCKVCMDKDVSVVFVPCGHLVACEECALNLRLCPICRAGIQGRVRAF 311
[Close]
BLAST of SMED30031552 vs. Ensembl Sea Lamprey
Match: birc2 (baculoviral IAP repeat containing 2 [Source:ZFIN;Acc:ZDB-GENE-030825-6])

HSP 1 Score: 51.2174 bits (121), Expect = 1.254e-6
Identity = 22/65 (33.85%), Postives = 34/65 (52.31%), Query Frame = -1 
Query:  118 PKESLEKSMIKKAILEQHQCVTCWQKHAEIIYKPCGHMVNSEACGLNVNLCPVCRQQINGRIKGF 312
            P+E L      K + EQH C  C  K   +++ PCGH+V    C  ++  CP+CR  + G ++ F
Sbjct:  516 PEEEL------KRLQEQHTCKVCMDKEVSVVFIPCGHLVVCRDCASSLRKCPICRAVVRGTVRTF 574
[Close]
BLAST of SMED30031552 vs. Planmine SMEST
Match: SMESG000023758.1 (SMESG000023758.1)

HSP 1 Score: 207.608 bits (527), Expect = 9.874e-89
Identity = 96/117 (82.05%), Postives = 104/117 (88.89%), Query Frame = -1 
Query:  103 KTRTLMLLSGVKTNTMRKIISERLANNLEWFDSTQAVIRAIKRYQEKPKESLEKSMIKKAILEQHQCVTCWQKHAEIIYKPCGHMVNSEACGLNVNLCPVCRQQINGRIKGFYVADQ 453
            KTRT+MLLSGV T  MRKII ERLANNLEW+DSTQAVIRAIKRYQEKPKE+ +KSMIK+AILEQ+QCVTCWQKH EIIY PCGHMV  EACGL VN+CPVCR+ INGRIKGF  ADQ
Sbjct: 4309 KTRTVMLLSGVDTQKMRKIIKERLANNLEWYDSTQAVIRAIKRYQEKPKENRDKSMIKEAILEQYQCVTCWQKHVEIIYIPCGHMVTCEACGLKVNICPVCRKPINGRIKGFRFADQ 4425

HSP 2 Score: 140.969 bits (354), Expect = 9.874e-89
Identity = 82/94 (87.23%), Postives = 88/94 (93.62%), Query Frame = -2 
Query:  450 ELIWEGETIKRTFEERIKTFKGHPRAAEFASQGYCQIARGIMCEGCKNQQPIPDQNVXXXXXXXXXXXXXXXXXKNLEVVITNQQVEDILDRQK 731
            ELIWE ETI RTFEERIKTFKG+PRAAEFASQGYCQ ARGIMCEGCKNQQPIPDQNVKKLKHK KC YK+EKESKN+E++ITNQQ+EDILDR K
Sbjct: 4216 ELIWEKETINRTFEERIKTFKGNPRAAEFASQGYCQTARGIMCEGCKNQQPIPDQNVKKLKHKGKCMYKQEKESKNIEIIITNQQIEDILDRPK 4309
[Close]
BLAST of SMED30031552 vs. Planmine SMEST
Match: SMESG000025043.1 (SMESG000025043.1)

HSP 1 Score: 204.142 bits (518), Expect = 2.196e-87
Identity = 94/117 (80.34%), Postives = 103/117 (88.03%), Query Frame = -1 
Query:  103 KTRTLMLLSGVKTNTMRKIISERLANNLEWFDSTQAVIRAIKRYQEKPKESLEKSMIKKAILEQHQCVTCWQKHAEIIYKPCGHMVNSEACGLNVNLCPVCRQQINGRIKGFYVADQ 453
            KTRT+MLLSGV T  MRKII ERLANN+EW+DSTQAVIRAIKRYQEKPKE+ +KSMIK+AILEQ+QCVTCWQKH EIIY PCGHMV  EACGL V +CPVCR+ INGRIKGF  ADQ
Sbjct: 1746 KTRTIMLLSGVDTQKMRKIIKERLANNVEWYDSTQAVIRAIKRYQEKPKENRDKSMIKEAILEQYQCVTCWQKHVEIIYTPCGHMVTCEACGLKVEICPVCRKPINGRIKGFRFADQ 1862

HSP 2 Score: 140.198 bits (352), Expect = 2.196e-87
Identity = 81/94 (86.17%), Postives = 88/94 (93.62%), Query Frame = -2 
Query:  450 ELIWEGETIKRTFEERIKTFKGHPRAAEFASQGYCQIARGIMCEGCKNQQPIPDQNVXXXXXXXXXXXXXXXXXKNLEVVITNQQVEDILDRQK 731
            ELIWE ETI RTFEERIKTFKG+PRAAEFASQGYCQ ARGIMCEGCKNQQP+PDQNVKKLKHK KC YK+EKESKN+E++ITNQQ+EDILDR K
Sbjct: 1653 ELIWEKETINRTFEERIKTFKGNPRAAEFASQGYCQTARGIMCEGCKNQQPVPDQNVKKLKHKGKCMYKQEKESKNIEIIITNQQIEDILDRPK 1746
[Close]
BLAST of SMED30031552 vs. Planmine SMEST
Match: SMESG000025043.1 (SMESG000025043.1)

HSP 1 Score: 204.142 bits (518), Expect = 2.233e-87
Identity = 94/117 (80.34%), Postives = 103/117 (88.03%), Query Frame = -1 
Query:  103 KTRTLMLLSGVKTNTMRKIISERLANNLEWFDSTQAVIRAIKRYQEKPKESLEKSMIKKAILEQHQCVTCWQKHAEIIYKPCGHMVNSEACGLNVNLCPVCRQQINGRIKGFYVADQ 453
            KTRT+MLLSGV T  MRKII ERLANN+EW+DSTQAVIRAIKRYQEKPKE+ +KSMIK+AILEQ+QCVTCWQKH EIIY PCGHMV  EACGL V +CPVCR+ INGRIKGF  ADQ
Sbjct: 1701 KTRTIMLLSGVDTQKMRKIIKERLANNVEWYDSTQAVIRAIKRYQEKPKENRDKSMIKEAILEQYQCVTCWQKHVEIIYTPCGHMVTCEACGLKVEICPVCRKPINGRIKGFRFADQ 1817

HSP 2 Score: 140.198 bits (352), Expect = 2.233e-87
Identity = 81/94 (86.17%), Postives = 88/94 (93.62%), Query Frame = -2 
Query:  450 ELIWEGETIKRTFEERIKTFKGHPRAAEFASQGYCQIARGIMCEGCKNQQPIPDQNVXXXXXXXXXXXXXXXXXKNLEVVITNQQVEDILDRQK 731
            ELIWE ETI RTFEERIKTFKG+PRAAEFASQGYCQ ARGIMCEGCKNQQP+PDQNVKKLKHK KC YK+EKESKN+E++ITNQQ+EDILDR K
Sbjct: 1608 ELIWEKETINRTFEERIKTFKGNPRAAEFASQGYCQTARGIMCEGCKNQQPVPDQNVKKLKHKGKCMYKQEKESKNIEIIITNQQIEDILDRPK 1701
[Close]
BLAST of SMED30031552 vs. Planmine SMEST
Match: SMESG000025043.1 (SMESG000025043.1)

HSP 1 Score: 203.756 bits (517), Expect = 2.327e-87
Identity = 94/117 (80.34%), Postives = 103/117 (88.03%), Query Frame = -1 
Query:  103 KTRTLMLLSGVKTNTMRKIISERLANNLEWFDSTQAVIRAIKRYQEKPKESLEKSMIKKAILEQHQCVTCWQKHAEIIYKPCGHMVNSEACGLNVNLCPVCRQQINGRIKGFYVADQ 453
            KTRT+MLLSGV T  MRKII ERLANN+EW+DSTQAVIRAIKRYQEKPKE+ +KSMIK+AILEQ+QCVTCWQKH EIIY PCGHMV  EACGL V +CPVCR+ INGRIKGF  ADQ
Sbjct: 1752 KTRTIMLLSGVDTQKMRKIIKERLANNVEWYDSTQAVIRAIKRYQEKPKENRDKSMIKEAILEQYQCVTCWQKHVEIIYTPCGHMVTCEACGLKVEICPVCRKPINGRIKGFRFADQ 1868

HSP 2 Score: 140.198 bits (352), Expect = 2.327e-87
Identity = 81/94 (86.17%), Postives = 88/94 (93.62%), Query Frame = -2 
Query:  450 ELIWEGETIKRTFEERIKTFKGHPRAAEFASQGYCQIARGIMCEGCKNQQPIPDQNVXXXXXXXXXXXXXXXXXKNLEVVITNQQVEDILDRQK 731
            ELIWE ETI RTFEERIKTFKG+PRAAEFASQGYCQ ARGIMCEGCKNQQP+PDQNVKKLKHK KC YK+EKESKN+E++ITNQQ+EDILDR K
Sbjct: 1659 ELIWEKETINRTFEERIKTFKGNPRAAEFASQGYCQTARGIMCEGCKNQQPVPDQNVKKLKHKGKCMYKQEKESKNIEIIITNQQIEDILDRPK 1752
[Close]
BLAST of SMED30031552 vs. Planmine SMEST
Match: SMESG000025043.1 (SMESG000025043.1)

HSP 1 Score: 204.142 bits (518), Expect = 2.327e-87
Identity = 94/117 (80.34%), Postives = 103/117 (88.03%), Query Frame = -1 
Query:  103 KTRTLMLLSGVKTNTMRKIISERLANNLEWFDSTQAVIRAIKRYQEKPKESLEKSMIKKAILEQHQCVTCWQKHAEIIYKPCGHMVNSEACGLNVNLCPVCRQQINGRIKGFYVADQ 453
            KTRT+MLLSGV T  MRKII ERLANN+EW+DSTQAVIRAIKRYQEKPKE+ +KSMIK+AILEQ+QCVTCWQKH EIIY PCGHMV  EACGL V +CPVCR+ INGRIKGF  ADQ
Sbjct: 1748 KTRTIMLLSGVDTQKMRKIIKERLANNVEWYDSTQAVIRAIKRYQEKPKENRDKSMIKEAILEQYQCVTCWQKHVEIIYTPCGHMVTCEACGLKVEICPVCRKPINGRIKGFRFADQ 1864

HSP 2 Score: 140.198 bits (352), Expect = 2.327e-87
Identity = 81/94 (86.17%), Postives = 88/94 (93.62%), Query Frame = -2 
Query:  450 ELIWEGETIKRTFEERIKTFKGHPRAAEFASQGYCQIARGIMCEGCKNQQPIPDQNVXXXXXXXXXXXXXXXXXKNLEVVITNQQVEDILDRQK 731
            ELIWE ETI RTFEERIKTFKG+PRAAEFASQGYCQ ARGIMCEGCKNQQP+PDQNVKKLKHK KC YK+EKESKN+E++ITNQQ+EDILDR K
Sbjct: 1655 ELIWEKETINRTFEERIKTFKGNPRAAEFASQGYCQTARGIMCEGCKNQQPVPDQNVKKLKHKGKCMYKQEKESKNIEIIITNQQIEDILDRPK 1748
The following BLAST results are available for this feature:
BLAST of SMED30031552 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031552 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031552 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031552 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031552 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031552 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031552 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|Q8JHV9|BIR7A_XENLA1.563e-629.20Baculoviral IAP repeat-containing protein 7-A OS=X... [more]
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BLAST of SMED30031552 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
A0A218USP63.656e-630.84Baculoviral IAP repeat-containing protein 7-B OS=L... [more]
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BLAST of SMED30031552 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031552 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
birc21.254e-633.85baculoviral IAP repeat containing 2 [Source:ZFIN;A... [more]
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BLAST of SMED30031552 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031552 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031552 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031552 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000023758.19.874e-8982.05SMESG000023758.1[more]
SMESG000025043.12.196e-8780.34SMESG000025043.1[more]
SMESG000025043.12.233e-8780.34SMESG000025043.1[more]
SMESG000025043.12.327e-8780.34SMESG000025043.1[more]
SMESG000025043.12.327e-8780.34SMESG000025043.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031552 ID=SMED30031552|Name=SMED30031552|organism=Schmidtea mediterranea sexual|type=transcript|length=3813bp
CCGTATTTCGAATTTTAAGGATTTTTAGTTTTATAATTTTTTTTATCTTT
AACGTTTTATTTACTATGTACATTTTTACACATTGTTTTCATCCTCTTTT
TACTGATCAGCGACATAAAATCCCTTGATCCGTCCGTTGATCTGTTGTCT
GCATACTGGACATAAATTCACGTTTAATCCACATGCTTCGGAGTTGACCA
TATGTCCACAGGGTTTATAAATTATTTCGGCATGTTTTTGCCAGCATGTT
ACACATTGATGCTGTTCTAATATGGCTTTTTTTATCATTGACTTTTCCAG
GCTTTCTTTTGGTTTCTCTTGGTAACGTTTGATGGCTCGTATTACTGCTT
GCGTTGAGTCAAACCATTCTAGGTTGTTAGCTAATCGTTCACTGATAATT
TTTCTCATTGTGTTGGTTTTTACTCCGGATAGTAACATTAAGGTCCTGGT
TTTTGTCGATCTAATATATCTTCTACTTGTTGATTTGTGATTACTACTTC
TAAGTTTTTTGATTCCTTTTCCTCTTTATACTTACATTTTTCTTTATGTT
TCAATTTTTTAACGTTTTGATCTGGTATTGGCTGCTGGTTTTTGCATCCC
TCGCACATTATTCCTCGTGCTATTTGGCAATATCCTTGGCTTGCAAATTC
CGCTGCTCTTGGATGTCCTTTGAAAGTTTTTATCCTTTCTTCGAAGGTTC
TCTTTATAGTCTCGCCTTCCCATATTAATTCTTGCCCCCAATGGTCACCC
GTTTCTAGGCAACGTTGGAAATGTAACCATTGGTTTGAGGTATCCAAAGG
TGAGCTTTACGAACGAACTGCTCTGGCCCAACCTCAAAGCGGGTCCAACG
CACTACCGATAGAAGGTTTGTGTGCGCTTTACTTATTCTCGTCCCATCTG
CATGTCTCAGTTGGTCTTGTCGCGTAAGATAACTTCCCTTTGATTTGTAT
TTACGTTATTCATCTCTCAACGTTAAAAATGATCAACAATTTAATCAACA
CAAATTTATTTTTAAAATTAACGACAAAAACGTCCTAATCGGCTGGTTGA
GCGACTCGACGTTTGGCGCGCTCATTTCAACCAGGTCGGTGTTGAGGCGG
TTTACTTGCAGGTTTGGAGTTTGATGTATATTCTTTGCAGGCCGATTGGG
CTGGAGCAAAAACCAACGGGTAAGTCACAGCATTGCCTTCCTGGCATGCA
GACAAAGCCACTTCTAGCCCGCGGATTTGGGTCTTCTGATTTTGGTTTAA
TTGCTTTTCTTCGGCCCAACCTCGTTGAGCTGCCGTCATCTTTTGGTATA
GAACCTTAACTTTCTTTGGCTCCACCCAACAAGATTTGACTCGCTGAATT
TCCGTCGTCAGTTGCACAATCTGCTGGTGCAATTCTAGATTTAGTTTTTG
AGCTTTGGTCAATTCTTCTTGTAGAATTTTGTGGGATTCCATGTCTAGAA
CGTGATAAACCATTAAACTCTCCTTCACTATATTAATTCCTAGTTTTATC
TACAAACAACACAATGCTTAATCTCGTGGTGGTCCTTATAAGTAACAAAT
TCAATTAAGGAATAACAAAACATTAACAAGAAAATCCAATTTTTATTTTC
AACTAATTAAATTTCCCATATCTTCCGTTTCCGGATCGTCTCGGTATCTC
ATGACCATAAGAGTGTTCCTGGTCCTGGTGGGGGACGTTTGTCTGCGTCC
ATTATCTCACTGGACAACATTCCTCGCGGGATCACCATGTTCATGGTTTC
TGTTTCTCCCGCCAATCTCGGGTCGTCAAGGTTGTAGGAGACATTGTACG
ATGGCCCGTACCCATCCGTACTTGGAATAGCACCAATTGCTATGGCCCGA
TTTACTTCGGCTAGATATCGTCTGGCCCGGTCCACCTGTCGGTCGGTTTG
GCGTTGGTGTTCCTCCAAACGCGCCAACAGAAATTCCGCTTGCCCTCGAA
TTTCTCGGGCTGGATTACGGCCCGGCTCTATTTTGATCGCCACAAAAGGG
AGTTCTATGTCCGCGTCGTCTCTATTTGTGCCCGCGCGGGATTCTTTCAC
TGACGAGACTGAGAATCCTGCGTCCCATAGGGCCTGTCTGCTTTTGGCGA
CTGATCGTGCGGGAAACCGCACAGTGACGCGGGACGCGTCTATCCAAGAA
CGATCTGCAAAAGGTTAATCATCAGATTATTGAATAATTCATCGATATAT
ATCTTTTATTATGAACTTTTATTACAAATTTAATTTATGTGTGACAATTA
CATGTACTAATAATATATTTATGGGATGGATGATCAGCTAAGAATGATTA
TTGGGTTCTCGGACGCGTATTCATACGAATGCTTTCCTCGAAGTAAGCTA
TTTTCTACCCCTCGTATTTTTAGAAAATATACATTTATCCTACTTGCTCG
ACTCAGACGGTTATTCATAGTTATCTCAAGGGTGTTTCGGAATTCATAAG
GACTTGCCCTTGTCTGTGAGTTGTTACTCGCTATCAGAAACAAAACATTC
GTCGTCGGAATTATTTGGACTCTCGCGGAAATCTTAATCGTCCATAATGG
GCTACGATGTTACCAGAATTGTAGTCGATAATCCCTAATTTTCGCCGTAG
ACTAATCTCTCAACGAGTACGCTGTTCCACTTCTGAATTGCTGGGCATCG
ATTAGGTAGACGAGTCAAAGGGTGGACATTTTAGAAAGATTGCCTCATGA
AAATAACTCCATTTAACAGTGTCAAAAAACATTAACTCCATAACACGGTT
AATGATAATTTTATTTAATATGTCAACCAAATCCTTGCGATCCTACGGAA
TGTTTACTGGACAGACATTACGTTTACTGGCGGCGAATTTTCTCTGGAAA
TCAAATGAGATCTAATCGTTTCTGTCCTCTGTGATGGCACTCATATAAAG
GGGTAAATTAAATGGATCAAATGATATGAGTAAAGCGCTTACCGTCTTCT
GGCGAAGATCGCCTCTTTTCCATTGTGAAGCCAGGTTCTCCTCGAAAACC
GCTAGTTCCTCCATTTCTCTGCCAATTATCTCGAGCGCTCATAGTAGTTT
GCTGCAATCGCGTATCAGATAAAATCGTTACCGTATTCATATTTATACTA
CACTCTAGGGACGTGCCACGTAGTCATTCTTTTCACTTTGGTAAATTCCG
TGTACGTGTAAATATTTTCGCCCTACACAATTTACGGCCACATCTATTCA
TATATTAATTGATCTTCGTGTTGTTTTCCTACTCAGATCGTCACATTTCT
ATTGATTTTCTTTCCTCTCCCCAATGATTGTTTGCATGAATATTATCGAC
ATTCCACTAGTGGTGTGTTGGGGAATTTCTGTCAAGTGTGGTTTGTTATT
TAATCTATGGGGAATTATATCAATTCATTTATTCTAATTTGAATGTTCAT
GTATTGTCAAGCGAAGATTAGCTCTCTGCTATCTCAAACTAACGAAAATA
TTATTCCAGTTTTCTTGTCGACAATTAAGTCAGCTACAAATGGCTGGTAT
AAAAGATTCGTTTCCAAAGGATTGGTCGATCTCGTTAAGGTGTTATACGA
ATACCGGAACTATTATTCAGTGGATTCAGTTACTGTTCCGATGAAGACGT
TACTGCGAAGATCACTTTCGAGAAATCCTAAAGTTCATCCGCTTATTCCA
TGATCCTCGTCGACTCGCAAATCATTTGTGATGTTTTTCGTGAATTAATT
GATTAAATGCTCTCAACAATTCTGTTGTGCAAGTCTTTTTCGTTGTATTT
TTCCCAATTTTGGTAGTTATTATGATTGTTTTTTGTTTATATTTTAATTA
AGGACTATTGATT
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protein sequence of SMED30031552-orf-1

>SMED30031552-orf-1 ID=SMED30031552-orf-1|Name=SMED30031552-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=129bp
MVYHVLDMESHKILQEELTKAQKLNLELHQQIVQLTTEIQRVKSCWVEPK
KVKVLYQKMTAAQRGWAEEKQLNQNQKTQIRGLEVALSACQEGNAVTYPL
VFAPAQSACKEYTSNSKPASKPPQHRPG*
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protein sequence of SMED30031552-orf-2

>SMED30031552-orf-2 ID=SMED30031552-orf-2|Name=SMED30031552-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=113bp
MLLSGVKTNTMRKIISERLANNLEWFDSTQAVIRAIKRYQEKPKESLEKS
MIKKAILEQHQCVTCWQKHAEIIYKPCGHMVNSEACGLNVNLCPVCRQQI
NGRIKGFYVADQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR013083Znf_RING/FYVE/PHD