Chromosome 19 open reading frame 10

Overview
NameChromosome 19 open reading frame 10
Smed IDSMED30031546
Length (bp)877
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Chromosome 19 open reading frame 10 (SMED30031546) t-SNE clustered cells

Violin plots show distribution of expression levels for Chromosome 19 open reading frame 10 (SMED30031546) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Chromosome 19 open reading frame 10 (SMED30031546) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Chromosome 19 open reading frame 10 (SMED30031546) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30031546

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
posterior sideSMED30031546SMESG000021061.1 dd_Smed_v4_5539_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30031546SMESG000021061.1 dd_Smed_v4_5539_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30031546SMESG000021061.1 OX_Smed_1.0.00817ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Chromosome 19 open reading frame 10 vs. Ensembl Human
Match: MYDGF (myeloid derived growth factor [Source:HGNC Symbol;Acc:HGNC:16948])

HSP 1 Score: 71.633 bits (174), Expect = 1.300e-14
Identity = 42/135 (31.11%), Postives = 74/135 (54.81%), Query Frame = 2 
Query:  164 DSASIDFQVTPGGIEHSAVH---TRDGAKCTFTYICQGGTNENWILAITKVKEQNTYGCYIQRKGGRSHSYIFFQKFSLSVTG-NVEF---HNGQAFKSD-DTKLAPAEYKVDKSSKSISQVDGKFGSQLNRVII 544
            +  ++ F V PGG+ HS  H     D   C FTY  QGGTNE W +++   ++   + C I R  G+  SY++F +F   V G  +E+   ++  AF+ + D  L   E++V K+  +++   G F ++L++++I
Sbjct:   34 EPTTVAFDVRPGGVVHSFSHNVGPGDKYTCMFTYASQGGTNEQWQMSLGTSEDHQHFTCTIWRPQGK--SYLYFTQFKAEVRGAEIEYAMAYSKAAFERESDVPLKTEEFEVTKT--AVAHRPGAFKAELSKLVI 164
[Close]
BLAST of Chromosome 19 open reading frame 10 vs. Ensembl Human
Match: MYDGF (myeloid derived growth factor [Source:HGNC Symbol;Acc:HGNC:16948])

HSP 1 Score: 62.3882 bits (150), Expect = 4.162e-11
Identity = 38/116 (32.76%), Postives = 62/116 (53.45%), Query Frame = 2 
Query:  164 DSASIDFQVTPGGIEHSAVH---TRDGAKCTFTYICQGGTNENWILAITKVKEQNTYGCYIQRKGGRSHSYIFFQKFSLSVTG-NVEF---HNGQAFKSD-DTKLAPAEYKVDKSS 487
            +  ++ F V PGG+ HS  H     D   C FTY  QGGTNE W +++   ++   + C I R  G+  SY++F +F   V G  +E+   ++  AF+ + D  L   E++V K++
Sbjct:   34 EPTTVAFDVRPGGVVHSFSHNVGPGDKYTCMFTYASQGGTNEQWQMSLGTSEDHQHFTCTIWRPQGK--SYLYFTQFKAEVRGAEIEYAMAYSKAAFERESDVPLKTEEFEVTKTA 147
[Close]
BLAST of Chromosome 19 open reading frame 10 vs. Ensembl Zebrafish
Match: mydgf (myeloid-derived growth factor [Source:ZFIN;Acc:ZDB-GENE-040718-183])

HSP 1 Score: 82.8037 bits (203), Expect = 5.411e-19
Identity = 45/135 (33.33%), Postives = 75/135 (55.56%), Query Frame = 2 
Query:  155 SSEDSASIDFQVTPGGIEHSAVHTRDGAKCTFTYICQGGTNENWILAITKVKEQNTYGCYIQRKGGRSHSYIFFQKFSLSVTG-NVEFHNGQAFKS----DDTKLAPAEYKVDKSSKSISQVDGKFGSQLNRVII 544
            S+E + ++DF V PGG+  +        KCTFTY CQGGTNE W +++    ++  + C + R  G+  SY+FF +F   + G  +E+    +  +     D  L   EY V +S  +++Q DGKF S+L+++ +
Sbjct:   25 SAERTKTLDFDVKPGGVVQTFSAKLKKYKCTFTYACQGGTNEQWQMSVGLSDDEQMFSCSVWRPQGK--SYLFFTQFKAEIKGAKIEYATAYSQTAVGGQRDVALKEEEYIVSES--AVTQRDGKFHSELSKLTV 155
[Close]
BLAST of Chromosome 19 open reading frame 10 vs. Ensembl Xenopus
Match: yes1 (YES proto-oncogene 1, Src family tyrosine kinase [Source:Xenbase;Acc:XB-GENE-495082])

HSP 1 Score: 77.7962 bits (190), Expect = 5.813e-17
Identity = 49/133 (36.84%), Postives = 69/133 (51.88%), Query Frame = 2 
Query:  161 EDSASIDFQVTPGGIEHSAVHTRDGAKCTFTYICQGGTNENWILAITKVKEQNTYGCYIQRKGGRSHSYIFFQKFSLSVT-GNVEFHNG--QAFK--SDDTKLAPAEYKVDKSSKSISQVDGKFGSQLNRVII 544
            E S++ +F V PGG++HS         CTFTY  QGGTNE W +++    +   + C I R  G+  SY+FF  F   VT G +EF     QA    S D KL  +EY  D +   +S   G F S L ++++
Sbjct:   21 EKSSTEEFDVRPGGLQHSFTSKLGDYACTFTYAAQGGTNEKWHMSVGLSDDNQHFSCSIWRPQGK--SYLFFTGFKAEVTGGKIEFSEAYSQASSDGSSDVKLKSSEY--DVTDNVVSHRPGSFSSSLCKLVL 149
[Close]
BLAST of Chromosome 19 open reading frame 10 vs. Ensembl Mouse
Match: Mydgf (myeloid derived growth factor [Source:MGI Symbol;Acc:MGI:2156020])

HSP 1 Score: 70.4774 bits (171), Expect = 2.314e-14
Identity = 42/135 (31.11%), Postives = 75/135 (55.56%), Query Frame = 2 
Query:  164 DSASIDFQVTPGGIEHS---AVHTRDGAKCTFTYICQGGTNENWILAITKVKEQNTYGCYIQRKGGRSHSYIFFQKFSLSVTG-NVEF---HNGQAFKSD-DTKLAPAEYKVDKSSKSISQVDGKFGSQLNRVII 544
            +  ++ F V PGG+ HS    V   +   CTFTY  QGGTNE W +++   ++   + C I R  G+  SY++F +F   + G  +E+   ++  AF+ + D  L   E++V K+  ++S   G F ++L++++I
Sbjct:   27 EPTTVPFDVRPGGVVHSFSQDVGPGNKFTCTFTYASQGGTNEQWQMSLGTSEDSQHFTCTIWRPQGK--SYLYFTQFKAELRGAEIEYAMAYSKAAFERESDVPLKSEEFEVTKT--AVSHRPGAFKAELSKLVI 157
[Close]
BLAST of Chromosome 19 open reading frame 10 vs. UniProt/SwissProt
Match: sp|Q969H8|MYDGF_HUMAN (Myeloid-derived growth factor OS=Homo sapiens OX=9606 GN=MYDGF PE=1 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 6.244e-14
Identity = 42/135 (31.11%), Postives = 74/135 (54.81%), Query Frame = 2 
Query:  164 DSASIDFQVTPGGIEHSAVH---TRDGAKCTFTYICQGGTNENWILAITKVKEQNTYGCYIQRKGGRSHSYIFFQKFSLSVTG-NVEF---HNGQAFKSD-DTKLAPAEYKVDKSSKSISQVDGKFGSQLNRVII 544
            +  ++ F V PGG+ HS  H     D   C FTY  QGGTNE W +++   ++   + C I R  G+  SY++F +F   V G  +E+   ++  AF+ + D  L   E++V K+  +++   G F ++L++++I
Sbjct:   34 EPTTVAFDVRPGGVVHSFSHNVGPGDKYTCMFTYASQGGTNEQWQMSLGTSEDHQHFTCTIWRPQGK--SYLYFTQFKAEVRGAEIEYAMAYSKAAFERESDVPLKTEEFEVTKT--AVAHRPGAFKAELSKLVI 164
[Close]
BLAST of Chromosome 19 open reading frame 10 vs. UniProt/SwissProt
Match: sp|Q9CPT4|MYDGF_MOUSE (Myeloid-derived growth factor OS=Mus musculus OX=10090 GN=Mydgf PE=1 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.618e-13
Identity = 42/135 (31.11%), Postives = 75/135 (55.56%), Query Frame = 2 
Query:  164 DSASIDFQVTPGGIEHS---AVHTRDGAKCTFTYICQGGTNENWILAITKVKEQNTYGCYIQRKGGRSHSYIFFQKFSLSVTG-NVEF---HNGQAFKSD-DTKLAPAEYKVDKSSKSISQVDGKFGSQLNRVII 544
            +  ++ F V PGG+ HS    V   +   CTFTY  QGGTNE W +++   ++   + C I R  G+  SY++F +F   + G  +E+   ++  AF+ + D  L   E++V K+  ++S   G F ++L++++I
Sbjct:   27 EPTTVPFDVRPGGVVHSFSQDVGPGNKFTCTFTYASQGGTNEQWQMSLGTSEDSQHFTCTIWRPQGK--SYLYFTQFKAELRGAEIEYAMAYSKAAFERESDVPLKSEEFEVTKT--AVSHRPGAFKAELSKLVI 157
[Close]
BLAST of Chromosome 19 open reading frame 10 vs. UniProt/SwissProt
Match: sp|P62248|MYDGF_BOVIN (Myeloid-derived growth factor OS=Bos taurus OX=9913 GN=MYDGF PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 2.151e-13
Identity = 43/136 (31.62%), Postives = 69/136 (50.74%), Query Frame = 2 
Query:  164 DSASIDFQVTPGGIEHS---AVHTRDGAKCTFTYICQGGTNENWILAITKVKEQNTYGCYIQRKGGRSHSYIFFQKFSLSVTGNVEFHNGQAF------KSDDTKLAPAEYKVDKSSKSISQVDGKFGSQLNRVII 544
            +  ++ F V PGG+ HS    V   D   C FTY  QGGTNE W +++   ++   + C I R  G+  SY++F +F   V G  E   G A+      K  D  L   E++V K+  ++    G F ++L++++I
Sbjct:   35 EPTTVAFDVRPGGVVHSFSQNVGPGDKYTCVFTYASQGGTNEKWQMSLGTSEDHQHFTCTIWRPQGK--SYLYFTQFKAEVRG-AEIEYGMAYSKAAFEKESDVPLKNEEFEVTKT--AVFHRPGAFKAELSKLVI 165
[Close]
BLAST of Chromosome 19 open reading frame 10 vs. UniProt/SwissProt
Match: sp|Q54XC4|MYDGF_DICDI (Myeloid-derived growth factor homolog OS=Dictyostelium discoideum OX=44689 GN=DDB_G0279047 PE=3 SV=2)

HSP 1 Score: 48.521 bits (114), Expect = 7.716e-6
Identity = 28/81 (34.57%), Postives = 42/81 (51.85%), Query Frame = 2 
Query:  146 NSVSSEDSASIDFQVTPGGIEHSAVHTRDGAKCTFTYICQGGTNENWILAITKVKEQNTYGCYIQRKGGRSHSYIFFQKFS 388
            NS+S E     +F + P G   +     +  KC F++   GGTNENW + +T   E     C+I R   +  SY+FF++FS
Sbjct:   19 NSLSEEIDK--EFNIKPEGNLDTVELVLENYKCIFSFAATGGTNENWKIFLT--TENGVATCFIGRP--KPISYLFFKQFS 93
[Close]
BLAST of Chromosome 19 open reading frame 10 vs. TrEMBL
Match: A0A267DPQ2 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig006048g1 PE=4 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 4.016e-35
Identity = 60/132 (45.45%), Postives = 86/132 (65.15%), Query Frame = 2 
Query:  182 FQVTPGGIEHSAVHTRDGAKCTFTYICQGGTNENWILAITKVKEQNTYGCYIQRKGGRSHSYIFFQKFSLSVTGNVEFHNGQAFKSDDTKLAPAEYKVDKSSKSISQVDGKFGSQLNRVIIEYTVFGKSKEL 577
            F V PGG++H     RDG KCTFTY+CQGGTNE W + IT++ E  TYGC ++R+ G   SY+FFQ F+L++ G  E H   AF +   +L P E+K DK++  +   D KFG++L R+ +E++  GK  +L
Sbjct:   36 FDVKPGGVQHEFSMERDGIKCTFTYVCQGGTNEKWRMLITQIHEVGTYGCVVERESGT--SYLFFQSFTLALLGEAEAHQAHAFGNGGRELKPEEFKFDKAAAKVQNSD-KFGAELARLALEFSATGKRSDL 164
[Close]
BLAST of Chromosome 19 open reading frame 10 vs. TrEMBL
Match: A0A1I8H6P0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig016417g1 PE=4 SV=1)

HSP 1 Score: 133.65 bits (335), Expect = 6.036e-35
Identity = 60/132 (45.45%), Postives = 86/132 (65.15%), Query Frame = 2 
Query:  182 FQVTPGGIEHSAVHTRDGAKCTFTYICQGGTNENWILAITKVKEQNTYGCYIQRKGGRSHSYIFFQKFSLSVTGNVEFHNGQAFKSDDTKLAPAEYKVDKSSKSISQVDGKFGSQLNRVIIEYTVFGKSKEL 577
            F V PGG++H     RDG KCTFTY+CQGGTNE W + IT++ E  TYGC ++R+ G   SY+FFQ F+L++ G  E H   AF +   +L P E+K DK++  +   D KFG++L R+ +E++  GK  +L
Sbjct:   36 FDVKPGGVQHEFSMERDGIKCTFTYMCQGGTNEKWRMLITQIHEVGTYGCVVERESGT--SYLFFQSFTLALQGEAEAHQAHAFGNGGRELKPEEFKFDKAAAKVQNSD-KFGAELARLALEFSATGKRSDL 164
[Close]
BLAST of Chromosome 19 open reading frame 10 vs. TrEMBL
Match: A0A5K4F7H2 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 6.468e-30
Identity = 60/140 (42.86%), Postives = 89/140 (63.57%), Query Frame = 2 
Query:  164 DSASI--DFQVTPGGIEHSAVHTRDGAKCTFTYICQGGTNENWILAITKVKEQNTYGCYIQRKGGRSHSYIFFQKFSLSVTGNVEFHNGQAFKSDDTKLAPAEYKVDKSSKSISQVDGKFGSQLNRVIIEYTVFGKSKEL 577
            DS+SI  +F V PGG ++S+   RDG KCTFTY CQGGTNE W + ++K+++   YGC ++R G ++ SYI+FQ F L VT  VE  +  AF +++  L+  EY  +K+ + +S V GKF + L R+ +E+       EL
Sbjct:   18 DSSSIKKEFSVQPGGKKYSSTIERDGIKCTFTYECQGGTNEKWHMILSKIRDDKGYGCVVERSGSQT-SYIYFQSFKLDVTPPVEALSASAFGNNNQPLSDEEYYFNKNERYVSHVSGKFKAALTRLSLEFVSTPMKPEL 156
[Close]
BLAST of Chromosome 19 open reading frame 10 vs. TrEMBL
Match: A0A095ACH5 (UPF0556 protein C19orf10-like protein (Fragment) OS=Schistosoma haematobium OX=6185 GN=MS3_11207 PE=4 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 2.669e-29
Identity = 60/131 (45.80%), Postives = 84/131 (64.12%), Query Frame = 2 
Query:  164 DSASI--DFQVTPGGIEHSAVHTRDGAKCTFTYICQGGTNENWILAITKVKEQNTYGCYIQRKGGRSHSYIFFQKFSLSVTGNVEFHNGQAFKSDDTKLAPAEYKVDKSSKSISQVDGKFGSQLNRVIIEY 550
            D +SI  +F V PGG ++S+   RDG KCTFTY CQGGTNE W + ++K+++ N YGC ++R G ++ SYIFFQ F L VT  VE     AF + +  LA  EY  +K+   +S V GKF + L R+ +E+
Sbjct:   18 DGSSIKKEFSVQPGGKKYSSTIERDGIKCTFTYECQGGTNEKWHMILSKIRDDNGYGCVVERSGSQT-SYIFFQSFKLDVTPPVEALTASAFGNGNQPLAAEEYYFNKNECFVSHVGGKFRAALTRLSLEF 147
[Close]
BLAST of Chromosome 19 open reading frame 10 vs. TrEMBL
Match: A0A5K3FQC7 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 9.106e-24
Identity = 61/149 (40.94%), Postives = 81/149 (54.36%), Query Frame = 2 
Query:  146 NSVSSEDSASIDFQ-----VTPGGIEHSAVHTRDGAKCTFTYICQGGTNENWILAITKVKEQNTYGCYIQRKGGRSHSYIFFQKFSLSVTGNVEFHNGQAFKSDDTKLAPAEYKVDKSSKSISQVDGKFGSQLNRVIIEYTVFGKSKEL 577
            +SV S D+AS + +     V PGG+  +      G  CTF Y CQGGT E W L+I   ++Q+ Y C++QR G  S SY+FFQKF ++V       +G AF      L PAEY VD    SIS V GKF   L  V +E+ +  +  EL
Sbjct:   13 HSVLSSDTASKNIKIKEISVKPGGVTRTESIEGFGVVCTFEYSCQGGTGEGWHLSIVYSEKQDRYVCHVQRTG-NSISYLFFQKFVMTVADPAAMTSGVAFDDKSKLLDPAEYFVDTKRNSISHVGGKFKGHLGHVSLEFEMKSRRPEL 160
[Close]
BLAST of Chromosome 19 open reading frame 10 vs. Ensembl Cavefish
Match: mydgf (myeloid derived growth factor [Source:NCBI gene;Acc:103036454])

HSP 1 Score: 73.559 bits (179), Expect = 1.281e-15
Identity = 43/144 (29.86%), Postives = 76/144 (52.78%), Query Frame = 2 
Query:  155 SSEDSASIDFQVTPGGIEHSAVHTRDGAKCTFTYICQGGTNENWILAITKVKEQNTYGCYIQRKGGRSHSYIFFQKFSLSVTG-NVEFHNGQAFKS----DDTKLAPAEYKVDKSSKSISQVDGKFGSQLNRVIIEYTVFGKSK 571
            S E + ++DF V PGG+ HS        +C FTY  QGGTNE W +++    +   + C + R  G+  SY+FF +F   + G  +E+ +  +  +     D  L   EY V  +  +++  DGKF ++L+++    T+ G+++
Sbjct:   30 SGESAKTVDFNVKPGGVVHSFSEKIKEYECIFTYASQGGTNEQWQMSVGLSDDDRLFSCSVWRPQGK--SYLFFTQFKAEIKGAKIEYASAYSQAAVGGQKDVPLKDEEYAVGDT--TVTHKDGKFRAELSKL----TLIGRTR 165
[Close]
BLAST of Chromosome 19 open reading frame 10 vs. Ensembl Medaka
Match: mydgf (myeloid derived growth factor [Source:NCBI gene;Acc:101166110])

HSP 1 Score: 74.7146 bits (182), Expect = 2.176e-16
Identity = 40/127 (31.50%), Postives = 69/127 (54.33%), Query Frame = 2 
Query:  173 SIDFQVTPGGIEHSAVHTRDGAKCTFTYICQGGTNENWILAITKVKEQNTYGCYIQRKGGRSHSYIFFQKFSLSVTGN-VEFHNG----QAFKSDDTKLAPAEYKVDKSSKSISQVDGKFGSQLNRV 538
            +++F V PGG+ HS        +CTFTY  QGGTNE W++++    + + + C +  +G    SY+FF +F   + G  +E+ N      A    D  L P E+ V   ++S +  +G+F +QL+++
Sbjct:    4 TVEFNVKPGGVVHSFTEGVRDYECTFTYASQGGTNEQWLMSVGLSDDDSLFSCSVWPQG---KSYLFFTQFKAELKGTRIEYANAYSQIAAGGQSDVPLKPEEFTV---AESTTHKEGRFNAQLSKL 124
[Close]
BLAST of Chromosome 19 open reading frame 10 vs. Ensembl Medaka
Match: mydgf (myeloid derived growth factor [Source:NCBI gene;Acc:101166110])

HSP 1 Score: 74.7146 bits (182), Expect = 9.694e-16
Identity = 40/116 (34.48%), Postives = 63/116 (54.31%), Query Frame = 2 
Query:  155 SSEDSASIDFQVTPGGIEHSAVHTRDGAKCTFTYICQGGTNENWILAITKVKEQNTYGCYIQRKGGRSHSYIFFQKFSLSVTGN-VEFHNG----QAFKSDDTKLAPAEYKVDKSS 487
            SSE + +++F V PGG+ HS        +CTFTY  QGGTNE W++++    + + + C + R  G+  SY+FF +F   + G  +E+ N      A    D  L P E+ V +S+
Sbjct:   30 SSEKTKTVEFNVKPGGVVHSFTEGVRDYECTFTYASQGGTNEQWLMSVGLSDDDSLFSCSVWRPQGK--SYLFFTQFKAELKGTRIEYANAYSQIAAGGQSDVPLKPEEFTVAEST 143
[Close]
BLAST of Chromosome 19 open reading frame 10 vs. Planmine SMEST
Match: SMESG000021061.1 (SMESG000021061.1)

HSP 1 Score: 301.982 bits (772), Expect = 1.206e-104
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 2 
Query:  140 YLNSVSSEDSASIDFQVTPGGIEHSAVHTRDGAKCTFTYICQGGTNENWILAITKVKEQNTYGCYIQRKGGRSHSYIFFQKFSLSVTGNVEFHNGQAFKSDDTKLAPAEYKVDKSSKSISQVDGKFGSQLNRVIIEYTVFGKSKEL 577
            YLNSVSSEDSASIDFQVTPGGIEHSAVHTRDGAKCTFTYICQGGTNENWILAITKVKEQNTYGCYIQRKGGRSHSYIFFQKFSLSVTGNVEFHNGQAFKSDDTKLAPAEYKVDKSSKSISQVDGKFGSQLNRVIIEYTVFGKSKEL
Sbjct:   11 YLNSVSSEDSASIDFQVTPGGIEHSAVHTRDGAKCTFTYICQGGTNENWILAITKVKEQNTYGCYIQRKGGRSHSYIFFQKFSLSVTGNVEFHNGQAFKSDDTKLAPAEYKVDKSSKSISQVDGKFGSQLNRVIIEYTVFGKSKEL 156
The following BLAST results are available for this feature:
BLAST of Chromosome 19 open reading frame 10 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
MYDGF1.300e-1431.11myeloid derived growth factor [Source:HGNC Symbol;... [more]
MYDGF4.162e-1132.76myeloid derived growth factor [Source:HGNC Symbol;... [more]
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BLAST of Chromosome 19 open reading frame 10 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Chromosome 19 open reading frame 10 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Chromosome 19 open reading frame 10 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mydgf5.411e-1933.33myeloid-derived growth factor [Source:ZFIN;Acc:ZDB... [more]
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BLAST of Chromosome 19 open reading frame 10 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
yes15.813e-1736.84YES proto-oncogene 1, Src family tyrosine kinase [... [more]
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BLAST of Chromosome 19 open reading frame 10 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Mydgf2.314e-1431.11myeloid derived growth factor [Source:MGI Symbol;A... [more]
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BLAST of Chromosome 19 open reading frame 10 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q969H8|MYDGF_HUMAN6.244e-1431.11Myeloid-derived growth factor OS=Homo sapiens OX=9... [more]
sp|Q9CPT4|MYDGF_MOUSE1.618e-1331.11Myeloid-derived growth factor OS=Mus musculus OX=1... [more]
sp|P62248|MYDGF_BOVIN2.151e-1331.62Myeloid-derived growth factor OS=Bos taurus OX=991... [more]
sp|Q54XC4|MYDGF_DICDI7.716e-634.57Myeloid-derived growth factor homolog OS=Dictyoste... [more]
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BLAST of Chromosome 19 open reading frame 10 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267DPQ24.016e-3545.45Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8H6P06.036e-3545.45Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A5K4F7H26.468e-3042.86Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A095ACH52.669e-2945.80UPF0556 protein C19orf10-like protein (Fragment) O... [more]
A0A5K3FQC79.106e-2440.94Uncharacterized protein OS=Mesocestoides corti OX=... [more]
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BLAST of Chromosome 19 open reading frame 10 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mydgf1.281e-1529.86myeloid derived growth factor [Source:NCBI gene;Ac... [more]
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BLAST of Chromosome 19 open reading frame 10 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Chromosome 19 open reading frame 10 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Chromosome 19 open reading frame 10 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Chromosome 19 open reading frame 10 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mydgf2.176e-1631.50myeloid derived growth factor [Source:NCBI gene;Ac... [more]
mydgf9.694e-1634.48myeloid derived growth factor [Source:NCBI gene;Ac... [more]
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BLAST of Chromosome 19 open reading frame 10 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000021061.11.206e-104100.00SMESG000021061.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031546 ID=SMED30031546|Name=Chromosome 19 open reading frame 10|organism=Schmidtea mediterranea sexual|type=transcript|length=877bp
ATTTGCGTTACACGTCATACTTTCTGTAAATATTGTTTAAACATATCAAT
TGCTATTTTTTATAAGTGCAGCAATATTCTTGAAAATTTGAAAACTTATT
TTTATCAAAATGAATTTCCTGTTTTTTATTTTTATTATATATCTGAATTC
AGTTTCTTCAGAAGATTCAGCGTCTATTGACTTTCAAGTAACACCAGGAG
GAATAGAGCACTCAGCAGTTCATACAAGGGATGGTGCAAAGTGCACATTT
ACTTACATATGCCAAGGTGGTACGAATGAAAATTGGATTCTGGCTATTAC
GAAAGTAAAAGAACAAAACACTTATGGTTGTTATATTCAACGAAAAGGTG
GTCGAAGTCACAGTTATATTTTCTTTCAAAAATTTAGTTTATCAGTTACT
GGAAATGTAGAATTTCACAACGGTCAAGCTTTTAAATCCGACGACACTAA
ATTGGCTCCAGCTGAATACAAAGTCGACAAAAGTTCAAAATCAATTTCAC
AAGTTGATGGCAAATTTGGATCTCAGTTAAATCGAGTTATCATCGAATAC
ACTGTATTTGGAAAATCTAAAGAACTTTAGAATAATTTTCGATTTTTCTC
TACTTTTTGATGGCCTCTTGTAAAAATTAATTTTATACCTTTGTTCTCTT
TTGTTTTCTAATATGGTTACATGATTACAAGTTTATACATTGTTGACAAA
AAGGTCAAATAAAGCAAAATGTATATTTTCCTGTCTGTACATTATTGTGT
CCGAAGAACTGGGTTTTCATTTCGTCTGTAATAATTAGCAACAAATAAAG
ATGTTATGTTCAAAGTTAAATATTGAAAGGAAACTTTATAAATAATTTAA
TAACGATGCCGCACTCTCAAAACAAAA
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protein sequence of SMED30031546-orf-1

>SMED30031546-orf-1 ID=SMED30031546-orf-1|Name=SMED30031546-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=157bp
MNFLFFIFIIYLNSVSSEDSASIDFQVTPGGIEHSAVHTRDGAKCTFTYI
CQGGTNENWILAITKVKEQNTYGCYIQRKGGRSHSYIFFQKFSLSVTGNV
EFHNGQAFKSDDTKLAPAEYKVDKSSKSISQVDGKFGSQLNRVIIEYTVF
GKSKEL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR018887MYDGF
Vocabulary: molecular function
TermDefinition
GO:0003674molecular_function
Vocabulary: biological process
TermDefinition
GO:0008150biological_process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018887Myeloid-derived growth factorPFAMPF10572UPF0556coord: 9..145
e-value: 2.2E-25
score: 88.9
IPR018887Myeloid-derived growth factorPANTHERPTHR31230FAMILY NOT NAMEDcoord: 4..146
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..17
score: 0.82