Outer membrane autotransporter barrel domain-containing protein

Overview
NameOuter membrane autotransporter barrel domain-containing protein
Smed IDSMED30031487
Length (bp)4520
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Outer membrane autotransporter barrel domain-containing protein (SMED30031487) t-SNE clustered cells

Violin plots show distribution of expression levels for Outer membrane autotransporter barrel domain-containing protein (SMED30031487) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Outer membrane autotransporter barrel domain-containing protein (SMED30031487) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Outer membrane autotransporter barrel domain-containing protein (SMED30031487) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30031487

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30031487h1SMcG0001319 dd_Smed_v4_10493_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30031487h1SMcG0001319 dd_Smed_v4_10493_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30031487h1SMcG0001319 dd_Smed_v4_10493_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Outer membrane autotransporter barrel domain-containing protein vs. Planmine SMEST
Match: SMESG000019226.1 (SMESG000019226.1)

HSP 1 Score: 2598.93 bits (6735), Expect = 0.000e+0
Identity = 1401/1408 (99.50%), Postives = 1402/1408 (99.57%), Query Frame = 1 
Query:   85 MIHNVFDREYWYNYFDVDLLLKFECKDQIIVSDAASSDALHQSHIISRVCKNLLDKDKFIKSAVIPINIGNVVDGIYEGSHWVGLVININPDKSFEALYADSFNENVNETLKTVLTNKGINAIKNLSQKQQNNVFDCGPWVVFNLISIARTRNLAVGIDEEMIXXXXXXXXXXXNDKKDECNTMNISDFPMENSVLINSTNLLKTNQTNELVSNDIDIIYEMKCSIINFLNNKLDLQNCLIEIGNLSEQISYNDKNDPQSKSIRNKYGFXXXXXXXXXXXXGIYFEKIFQLKIICSDDIVYSGIAQHEKDKLGRDXXXXXXXXXXXXXTENRNESINELESIPDGLFFIDNIVEIASKYYDIIIFREIRKTIKNIDNINFSIKEDRYFIARILTVIGQLLNEYLESKSINIKTNKLFIGFQDLMNIRNLTVHFHAKIKTFADNENQLSILNDSIINIRHHLENIITEDFQINSQYDNSENFIESTKIILNYLKFKSKPNSIVXXXXXXXXXXXXXXXXXXXXXXERMGSKTKDDSQLMKLNNEYQNLINQLDAQDKCDYPELLSISNKNILKEFINSMEKNMKGNKSEKAKKVKPIAKNNKVEMKSVIDDLLWKSYKIVSNNQINSNEIDKQLAPINQHYRSRINQLELSDRDFYPDEXXXXXXXXXXXXXEPKIRSKLLTSIKEINDEMSYLESTENNVELIEIKKMYICEHIVLVIGQIVRDLLNLGLCNYQSLNLPDEKWFVSRCISSEILSEAGRSARRSRNEGLAHNIWSFDSFKLWKIIINDLLPASNYLEKLFYINESETLPLSVYSSFTLGILFTDIGLYKRAIEYLQKALDLLNAVXXXXXXXXXXXXVCGMNQFLFQINANLAHVYRLNGQYQNATEILEFLLSKINYHEAILCENKIIKKEVSHVYVDICYLHILQLNYRKVIEICDQTILISDDLEIGYYLAIALWEDRQINRAIEIMENLKECNGDTIKSVMFIETIAFMCLDRLEIEKYFHLVVKCVEEMEKRMENNIMYYKENNPHGYLLEKIVIYYIKCKILMEESLQKLSYNIDSLKDIIKLSNKTIGKLNFDLKRDRIICYIYICFCKGYLCYFHFNEGKYHVLLEAEKFFNLAAEAADNLIERLPDIDQQLISTAEKLSDLYVICNEDVDTMLKIKYLEKSLSLQEKYNLNKLSSYHCLSRIFCNLGIDCYLDYMENRGQRSFDEALIDFXXXXXXXXXXXREPISENALEVIAMKDAVSKGISDYVNIETEKFLAMSYNRIGVLTYDVFVMKKSVRYYNKLLEMNLSVEAKSFYENQKTKCETRITSVISTLEFLVSDSRKIFVTDYSIEFLCWKFPNFVTESNCQELFKKTNQSLIDNLSPFINRELTKFSEEFFVLYKFYKLLIIYGLIRKFPEFP 4308
            MIHNVFDREYWYNYFDVDLLLKFECKDQIIVSDAASSDALHQSHIISRVCKNLLDKDKFIKSAVIPINIGNVVDGIYEGSHWVGLVININPDKSFEALYADSFNENVNETLKTVLTNKGINAIKNLSQKQQNNVFDCGPWVVFNLISIARTRNLAVGIDEEMILKQRLTLRLLLNDKKDECNTMNISDFPMENSVLINSTNLLKTNQTNELVSNDIDIIYEMKCSIINFLNNKLDLQNCLIEIGNLSEQISYNDKNDPQSKSIRNKYGFIQRNKNKQKNINGIYFEKIFQLKIICSDDIVYSGIAQHEKDKLGRDLINLKILLNKLLL ENRNESINELESIPDGLFFIDNIVEIASKYYDIIIFREIRKTIKNIDNINFSIKEDRYFIARILTVIGQLLNEYLESKSINIKTNKLFIGFQDLMNIRNLTVHFHAKIKTFADNENQLSILNDSIINIRHHLENIITE FQINSQYDNSENFIESTKIILNYLKFKSKPNSIVEKNSELIKINIKNLISKSVKIIERMGSKTKDDSQLMKLNNEYQNLINQLDAQDK DYPELLSISNKNILKEFINSMEKNMKGNKSEKAKKVKPIAKNNKVEMKSVIDDLLWKSYKIVSNNQINSNEIDKQLAPINQHYRSRINQLELSDRDFYPDEINY+NKNIIIKYNEPKIRSKLLTSIKEINDEMSYLESTENNVELIEIKKMYICEHIVLVIGQIVRDLLNLGLCNYQSLNLPDEKWFVSRCISSEILSEAGRSARRSRNEGLAHNIWSFDSFKLWKIIINDLLPASNYLEKLFYINESETLPLSVYSSFTLGILFTDIGLYKRAIEYLQKALDLLNAVSTISDTSSDYSSVCGMNQFLFQINANLAHVYRLNGQYQNATEILEFLLSKINY EAILCENKIIKKEVSHVYVDICYLHILQLNYRKVIEICDQTILISDDLEIGYYLAIALWEDRQINRAIEIMENLKECNGDTIKSVMFIETIAFMCLDRLEIEKYFHL VKCVEEMEKRMENNIMYYKENNPHGYLLEKIVIYYIKCKILMEESLQKLSYNIDSLKDIIKLSNKTIGKLNFDLKRDRIICYIYICFCKGYLCYFHFNEGKYHVLLEAEKFFNLAAEAADNLIERLPDIDQQLISTAEKLSDLYVICNEDVDTMLKIKYLEKSLSLQEKYNLNKLSSYHCLSRIFCNLGIDCYLDYMENRGQRSFDEALIDFKKSIKFCKLKKREPISENALEVIAMKDAVSKGISDYVNIETEKFLAMSYNRIGVLTYDVFVMKKSVRYYNKLLEMNLSVEAKSFYENQKTKCETRITSVISTLEFLVSDSRKIFVTDYSIEFLCWKFPNFVTESNCQELFKKTNQSLIDNLSPFINRELTKFSEEFFVLYKFYKLLIIYG IRKFPEFP
Sbjct:    1 MIHNVFDREYWYNYFDVDLLLKFECKDQIIVSDAASSDALHQSHIISRVCKNLLDKDKFIKSAVIPINIGNVVDGIYEGSHWVGLVININPDKSFEALYADSFNENVNETLKTVLTNKGINAIKNLSQKQQNNVFDCGPWVVFNLISIARTRNLAVGIDEEMILKQRLTLRLLLNDKKDECNTMNISDFPMENSVLINSTNLLKTNQTNELVSNDIDIIYEMKCSIINFLNNKLDLQNCLIEIGNLSEQISYNDKNDPQSKSIRNKYGFIQRNKNKQKNINGIYFEKIFQLKIICSDDIVYSGIAQHEKDKLGRDLINLKILLNKLLLIENRNESINELESIPDGLFFIDNIVEIASKYYDIIIFREIRKTIKNIDNINFSIKEDRYFIARILTVIGQLLNEYLESKSINIKTNKLFIGFQDLMNIRNLTVHFHAKIKTFADNENQLSILNDSIINIRHHLENIITEGFQINSQYDNSENFIESTKIILNYLKFKSKPNSIVEKNSELIKINIKNLISKSVKIIERMGSKTKDDSQLMKLNNEYQNLINQLDAQDKSDYPELLSISNKNILKEFINSMEKNMKGNKSEKAKKVKPIAKNNKVEMKSVIDDLLWKSYKIVSNNQINSNEIDKQLAPINQHYRSRINQLELSDRDFYPDEINYANKNIIIKYNEPKIRSKLLTSIKEINDEMSYLESTENNVELIEIKKMYICEHIVLVIGQIVRDLLNLGLCNYQSLNLPDEKWFVSRCISSEILSEAGRSARRSRNEGLAHNIWSFDSFKLWKIIINDLLPASNYLEKLFYINESETLPLSVYSSFTLGILFTDIGLYKRAIEYLQKALDLLNAVSTISDTSSDYSSVCGMNQFLFQINANLAHVYRLNGQYQNATEILEFLLSKINYQEAILCENKIIKKEVSHVYVDICYLHILQLNYRKVIEICDQTILISDDLEIGYYLAIALWEDRQINRAIEIMENLKECNGDTIKSVMFIETIAFMCLDRLEIEKYFHLAVKCVEEMEKRMENNIMYYKENNPHGYLLEKIVIYYIKCKILMEESLQKLSYNIDSLKDIIKLSNKTIGKLNFDLKRDRIICYIYICFCKGYLCYFHFNEGKYHVLLEAEKFFNLAAEAADNLIERLPDIDQQLISTAEKLSDLYVICNEDVDTMLKIKYLEKSLSLQEKYNLNKLSSYHCLSRIFCNLGIDCYLDYMENRGQRSFDEALIDFKKSIKFCKLKKREPISENALEVIAMKDAVSKGISDYVNIETEKFLAMSYNRIGVLTYDVFVMKKSVRYYNKLLEMNLSVEAKSFYENQKTKCETRITSVISTLEFLVSDSRKIFVTDYSIEFLCWKFPNFVTESNCQELFKKTNQSLIDNLSPFINRELTKFSEEFFVLYKFYKLLIIYGFIRKFPEFP 1408
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BLAST of Outer membrane autotransporter barrel domain-containing protein vs. Planmine SMEST
Match: SMESG000063585.1 (SMESG000063585.1)

HSP 1 Score: 98.9821 bits (245), Expect = 1.821e-20
Identity = 92/391 (23.53%), Postives = 186/391 (47.57%), Query Frame = 1 
Query: 2116 KLLTSIKEINDEMSYLESTENNVELIEIKKMYICEHIVLVIGQIVRDLLNLGLCNYQSLNLPDEKWFVSRCISSEILSEAGRSARRSRNEGLAHNIWSFDSFKLWKIIINDLLPASNYLEKLFYINESETLPLSVYSSFTLGILFTDIGLYKRAIEYLQKALDLLN-------------AVXXXXXXXXXXXXVCGMNQFLFQINANLAHVYRLNGQYQNATEILEFLLSKINYHEAILCENKIIKKEVSHVYVDICYLHILQLNYRKVIEICDQTILISDDLEIGYYLAIALWEDRQINRAIEIMENLKECNGDTIKSVM----FIETIAFMCLDRLEIEKYFHLVVKCVEEMEKRMENNIMYYKENNPHGYLLEKIVIYYIKCKILMEE 3237
            KL  ++K++  E  +L  +E +  L  +K+ ++ EHI+ VIGQI+R + +L   + + L +            S I+ EA +SA ++RN+GLAHN+++FD   L + I N++LPA+ + + +  I +     +S  S  +L + F ++G +  A++Y ++ L  ++              +       SD   + GM+ F F     LA  Y    +Y      LE L  ++        + + +   V+ +Y  + +  +++   RK I + ++ I I++      +LA A   +    +A +I++ L   +   ++  M    +   +A+    +  IE+ +    KC++EMEK    N   +KE     +++ K  +  ++ ++L+EE
Sbjct:  525 KLSDTMKQLVIESEFLVQSEKSNNLNRLKRPFVIEHIISVIGQIMRHISDLN-SHLKVLQM------------SSIIDEASKSATQARNQGLAHNVFAFDKDWLDEQIKNNILPANKHFQAILTIFKKNDCEVSRMSLISLALSFLNLGFFDSAVDYFRRTLKFISENPENKNQQCNLFELWFQLTIESDSEELLGMDMFSFICRLFLARTYVQACKYDEMFAELEILKERMQIKRIAKLKKESLLYYVNFLYHGLVHCLLIKGMNRKCISLLNEIIPITNFPIFEVHLAWAYHNENDYLQAYQIIKKLYPFDFKPLERYMVFYRYSRILAYQPNKKESIEESY----KCIDEMEKIFRENAREFKERYDDEFIMMKSHLVRLRVQLLVEE 898

HSP 2 Score: 95.5153 bits (236), Expect = 2.268e-19
Identity = 85/263 (32.32%), Postives = 140/263 (53.23%), Query Frame = 1 
Query:  700 NQTNELVSNDIDIIYEMKCSIINFLNNKLDLQNCLIEIGNLS---EQISYNDKNDPQSKSIRNKYGFXXXXXXXXXXXXGIYFEKIFQLKIICSDDIVY---SGIAQHEKDKLGRDXXXXXXXXXXXXXTENRNESIN-----ELESIPDGLFFIDNIVEIASKYYDIIIFREIRKTIKNIDNINFSIKEDRYFIARILTVIGQLLNEYLESKSINIKTNKLFIGFQDLMNIRNLTVHFHAKIKTFADNENQLSILNDSIINI 1455
            NQ  E+   DI  + EM+  I    NN++DL+  LI +G++S   ++I YNDK+   S  IRNKYG+ +R+ N   N+NGI FE ++Q+K++      Y   SG+   E +  G     L++  +   +    N+SI        ES P     +++I+   + +Y  II   I+  I  I   +F  KEDRY++AR LT+IG+L  E+L++ +  I+ +     F++L  +RN  +H+H+KI     N      +N+ I+ I
Sbjct:  103 NQNEEIRKLDIIRLKEMQEVISLLENNQMDLKTALISLGDISGGSKKIHYNDKD---SNCIRNKYGYFKRHDNCSINLNGINFEILYQVKLVLDSTNKYQLASGLKYIEDESKGLLADLLQVGKSLTHILSIENQSIQGEDKLSAESKPPQC-PMEHIINFVNNFYYPIILGRIKSEISQIGEFDFKSKEDRYYLARKLTIIGELAREFLDN-NFTIQNDNCKKVFKNLKMLRNKLIHYHSKISNGISNSEHSKDVNERILRI 360
[Close]
BLAST of Outer membrane autotransporter barrel domain-containing protein vs. Planmine SMEST
Match: SMESG000073364.1 (SMESG000073364.1)

HSP 1 Score: 95.5153 bits (236), Expect = 2.460e-19
Identity = 124/455 (27.25%), Postives = 213/455 (46.81%), Query Frame = 1 
Query:   85 MIHNVFDREYWYNYFDVDLLLKFECKDQIIVSDAASSDALHQSHIISRVCKNLLDKDKFIKSAVIPINIGNVV-DGIYEGSHWVGLVININPDKSFEALYADSFNE---NVNET--LKTVLTNKGINAIKNLSQKQQNNVFDCGPWVVFNLISIARTRNLAVGIDEEMIXXXXXXXXXXXNDKKDECNTMNISDFPMENSVLINSTNLLKTNQTNELVSNDIDIIYEMKCSIINFLNNKLDLQNCLIEIGNLSEQISYNDKNDPQSKSIRNKYGFXXXXXXXXXXXXGIYFEKIFQLKIICSDDIVYSGIAQHEKDK----LGRDXXXXXXXXXXXXXTENRNESINELESIPD-GLFFIDNIVEIASKYYDIIIFREIRKTIKNIDNINFSIKEDRYFIARILTVIGQLLNEYLESKSINIKTNKLFIGFQDLMNIRNLTVHFHAKIKTFADNE 1416
            M+ + ++R YW+N  ++++ L+ + +    V    S +   +  IIS +C+ L D +    +AV+ IN  +   D +     W GL I    D   +A + D F+    N N+   LK  L  KG + IK +S    + ++DCGP ++ +L  +ART      + E  I    L   +  N +    N    SD  + N  LI    ++  ++  ++              I+  ++N++DL++ L+EIG L E+IS N+ ++    SIR K+GF +R  + + N   I F+K  QLK    ++   S ++Q +  K    L      +  +L + L+  N NE I E+ES+ +  L   +  V I  +Y    +  +I+  +     IN++  EDRY   R+LT+IG L N+   S         +F   +    IRN  +H H KIK  + N+
Sbjct:    1 MVMDEYNRCYWFNNNEINIQLRKKSQGTYHVFQLESLNVTKE--IISEMCEKLKDHNG---AAVLSINYSSEKNDRLSHCPLWAGLAIIQAKDNIQKAFFIDPFDYFRYNFNKIFDLKIALLEKGFDNIKYISYWNNDKLYDCGPLIIIHLDYLARTGRSLDNVGEREI--TDLRSDVQNNYRMRNINKHQPSDLEISNQ-LIRKVKVIDMDKQEDIKI---------LKKILKLISNRIDLKSTLLEIGKLCEEISLNENDE---NSIRMKFGFSKRLADHENNNR-INFDKFCQLKAYIENE--SSLLSQSDLGKIENYLSSLKKKILFILKENLVFVNENEIIREIESLNEIDLLESNEFVSIVDRYQSEFVVSKIKTELNMTRGINYNNVEDRYVFFRVLTIIGNLSNQLDHS---------VFPSLKLCFKIRNKIIHCHKKIKENSKND 423

HSP 2 Score: 54.6842 bits (130), Expect = 7.318e-7
Identity = 108/448 (24.11%), Postives = 183/448 (40.85%), Query Frame = 1 
Query: 1672 KTKDDSQLMKLNNEYQNLINQLDAQDKCDYPELLSISNKNILKEFINSMEKNMKGNKSEKAKKVKPIAKNNKVEMKSVIDDLLWKSYKIVSNNQINSNEIDKQLAPINQHYRSRINQLELSDRDFYPDEXXXXXXXXXXXXXEP-----KIRSKLLTS-----------IKEINDEMSYLESTENNVELIEIKKMYICEHIVLVIGQIVRDLLNLGLCNYQSLNLPDEKWFVSRCISSEILSEAGRSARRSRNEGLAHNIWSFDSFKLWKIIINDL--LPASNYLEKLFYINESETLPLSVYSSFTLGILFTDIGLYKRAIEYLQKALDLLNAV----------------XXXXXXXXXXXXVCGMNQFLFQINANLAHVYRLNGQYQNATEILEFLLSKINYHEAILCEN-----KIIKKEVSHVYVDICYLHILQLNYRKVIEICD 2898
            K K++S+   LNNE  + I     Q   D  E   +  K  LK   N ++K  + NK  K   +K I      + K  +DD + K++K++  N       ++ L  +++ Y+ ++  L + D+  +P+ +      +      P      I   LL             I +I +E+ YL+  + +  +   KK YI  HI+ V+ Q VRDL ++ + +++   LP  KW     I                          FD+ +  KI I++     + +YL  L +I   E  P+     F +G+ F  + LY  AIEYL+ A + +N                       T++ Y +V G++ +   I   LA V  L    +N   + EF     + H+ I  +N     K +K E+   Y+D  YL I    Y + IEI +
Sbjct:  415 KIKENSKNDTLNNEKIHAIFNCIKQYLVDRGENTDLCTK--LKSLFNYIKK--RDNKPIKETSLKNI------DYKDKLDDFIRKAHKLIKRNP------NEDLRKLDESYQLQLGSLNIKDKRRFPENLINKINILEYIKKFPIKQNFDITENLLNQNILKRNIINYKISDIKNEIDYLKQIQLSNSITTDKKSYIIGHIICVVTQYVRDLSDIDISSFE---LPS-KWVKPTYI-------------------------PFDA-EFIKICISETNEYQSIDYLYTLPHI---EYCPIIALLLFNVGVSFAKLSLYDSAIEYLEMASENINVFIENSIHSDQTIIRNLGQHQKVTNNFYLAVLGIDPYELLIKRTLAKVLHLAN--KNDYSLKEF----NDVHQTIQLKNFYLADKFLKIEIIKFYIDYSYLLIDSHKYNEAIEIAE 807
The following BLAST results are available for this feature:
BLAST of Outer membrane autotransporter barrel domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Outer membrane autotransporter barrel domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Outer membrane autotransporter barrel domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Outer membrane autotransporter barrel domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Outer membrane autotransporter barrel domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Outer membrane autotransporter barrel domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Outer membrane autotransporter barrel domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Outer membrane autotransporter barrel domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Outer membrane autotransporter barrel domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Outer membrane autotransporter barrel domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Outer membrane autotransporter barrel domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Outer membrane autotransporter barrel domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Outer membrane autotransporter barrel domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Outer membrane autotransporter barrel domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000019226.10.000e+099.50SMESG000019226.1[more]
SMESG000063585.11.821e-2023.53SMESG000063585.1[more]
SMESG000073364.12.460e-1927.25SMESG000073364.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031487 ID=SMED30031487|Name=Outer membrane autotransporter barrel domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=4520bp
TTTAACTTAATTTATTATTAAAATGAGGATGCTAAATTGCTAAATATTTT
CCATTTTAGTTTTTGACTAATTTTACCAACAGAAATGATTCACAATGTTT
TTGATAGGGAATATTGGTACAATTATTTTGATGTTGATTTATTGTTAAAA
TTCGAATGCAAAGATCAAATTATTGTTAGCGATGCCGCGAGTTCTGATGC
GTTACATCAAAGTCATATAATCAGCAGGGTCTGTAAGAATTTACTTGATA
AAGATAAATTTATAAAATCTGCGGTTATCCCAATAAATATTGGAAATGTT
GTCGATGGAATTTACGAAGGTTCACATTGGGTTGGATTAGTAATAAATAT
TAACCCTGACAAATCTTTTGAGGCTCTGTATGCGGATTCATTTAATGAAA
ATGTCAATGAAACTTTGAAAACCGTCCTCACTAACAAAGGAATAAATGCA
ATTAAAAATTTATCCCAAAAACAACAGAATAATGTCTTTGATTGTGGGCC
ATGGGTCGTATTTAATTTAATAAGTATTGCAAGAACAAGAAATTTAGCGG
TTGGTATTGATGAAGAAATGATATTAAAACAGCGATTAACACTGCGGCTG
TTGTTGAATGATAAAAAAGACGAGTGCAATACAATGAACATATCCGATTT
TCCAATGGAAAATTCAGTTTTAATAAACTCAACAAATCTGTTAAAAACTA
ATCAAACAAATGAATTGGTATCAAATGACATTGATATTATTTATGAAATG
AAATGTTCAATAATCAATTTCCTGAATAACAAACTCGATTTGCAGAATTG
TCTGATTGAAATAGGCAATCTATCTGAACAGATATCATACAACGATAAAA
ATGATCCTCAAAGTAAATCGATTAGAAACAAATACGGATTTATTCAGAGA
AATAAAAATAAGCAAAAAAATATAAATGGCATATATTTTGAAAAGATATT
TCAATTGAAAATCATTTGTTCGGATGATATAGTATATTCTGGAATTGCTC
AACATGAAAAAGATAAACTAGGAAGAGATTTAATCAATTTAAAAATCCTA
TTAAATAAATTGTTATTAACAGAAAATCGAAATGAATCAATAAATGAGCT
AGAATCGATACCAGATGGATTGTTTTTTATCGATAATATCGTAGAAATCG
CTAGCAAATATTATGATATAATAATTTTCAGGGAAATTCGAAAGACTATA
AAAAACATCGATAATATTAATTTCAGTATCAAAGAAGATCGATATTTTAT
TGCTCGAATTCTGACTGTGATTGGTCAATTATTGAATGAATATTTAGAGA
GTAAATCAATTAATATAAAAACCAATAAATTATTTATTGGATTTCAAGAT
CTTATGAATATAAGAAATTTAACTGTTCATTTTCATGCGAAAATAAAAAC
TTTTGCCGATAATGAAAATCAGTTATCAATTCTTAACGATAGCATTATTA
ATATTAGACATCATTTGGAAAACATAATTACGGAAGATTTTCAAATAAAT
TCACAATATGATAATTCTGAAAATTTCATTGAATCGACTAAAATTATATT
GAATTATCTAAAATTCAAATCAAAGCCGAATTCAATAGTCGAAAAAAATA
GTGAATTAATTAAAATCAATATTAAAAACTTAATTTCCAAAAGCGTTAAG
ATTATCGAACGAATGGGATCGAAAACCAAAGATGACAGTCAGTTAATGAA
ATTAAATAATGAATATCAAAATTTAATAAATCAGCTGGATGCTCAAGATA
AATGTGATTACCCAGAATTATTGAGCATTTCCAATAAAAATATCCTTAAA
GAATTTATAAATTCAATGGAAAAAAATATGAAAGGAAATAAATCAGAAAA
AGCAAAAAAAGTAAAACCGATTGCTAAAAATAATAAAGTGGAAATGAAAT
CAGTAATTGACGATTTACTGTGGAAATCTTACAAAATTGTTAGCAATAAT
CAAATAAACAGTAACGAAATTGATAAACAATTGGCACCAATTAATCAGCA
TTATCGATCGAGAATAAACCAATTAGAACTTTCAGACAGAGATTTTTATC
CGGATGAAATAAATTACTCCAATAAAAATATCATTATTAAATACAATGAA
CCAAAAATAAGATCCAAATTGCTAACTTCAATTAAAGAAATCAATGATGA
AATGAGTTATTTGGAATCGACTGAAAACAATGTCGAATTAATAGAGATTA
AAAAAATGTATATTTGTGAGCACATTGTGTTGGTTATCGGACAAATTGTT
CGAGACTTGCTAAACTTGGGTCTGTGCAATTATCAGAGTTTGAATTTGCC
TGACGAAAAATGGTTTGTCAGCAGATGTATCTCATCGGAGATATTGAGTG
AAGCTGGGCGATCAGCTAGGCGTTCCCGAAATGAAGGATTGGCGCACAAC
ATCTGGTCATTTGACAGTTTTAAATTATGGAAAATCATTATTAACGATTT
ATTACCGGCCAGTAATTATCTGGAAAAGCTATTTTACATTAATGAAAGTG
AAACTCTACCATTGTCTGTTTATTCCTCTTTCACGTTAGGTATCTTATTT
ACTGATATTGGTCTGTACAAACGGGCAATTGAATATTTACAAAAAGCTCT
TGACCTGTTGAATGCGGTTTCAACTATTAGCGATACGTCAAGCGACTATA
GCTCTGTTTGTGGAATGAATCAATTCTTATTTCAAATCAATGCGAATTTA
GCACACGTTTATCGATTAAATGGTCAATATCAAAATGCGACGGAAATCCT
AGAATTTTTACTATCGAAAATAAATTACCATGAAGCTATTTTATGCGAAA
ATAAAATAATAAAAAAGGAAGTTAGTCACGTTTATGTTGATATATGTTAC
CTCCATATTCTTCAACTCAATTATAGAAAGGTTATTGAAATTTGTGATCA
AACAATTTTGATTTCTGATGATCTCGAAATCGGGTACTATTTGGCAATTG
CACTTTGGGAAGACAGGCAAATTAATCGAGCTATTGAAATAATGGAAAAT
CTTAAAGAATGTAACGGTGACACTATCAAATCTGTTATGTTTATTGAAAC
TATTGCTTTTATGTGTCTTGATAGACTGGAAATTGAAAAATATTTTCATC
TCGTTGTTAAATGTGTTGAGGAAATGGAAAAAAGAATGGAAAACAATATT
ATGTATTACAAAGAAAACAATCCTCATGGATATTTATTAGAGAAAATTGT
AATATATTACATCAAATGCAAAATTTTAATGGAAGAAAGTTTACAGAAAT
TGTCTTACAACATTGATTCGTTAAAGGATATCATAAAATTGTCCAATAAA
ACGATTGGGAAGTTAAATTTTGATTTGAAAAGAGATAGAATCATTTGTTA
TATTTACATTTGTTTCTGTAAAGGTTATTTGTGTTATTTCCATTTTAATG
AAGGAAAATATCACGTACTTCTTGAGGCTGAGAAATTTTTCAATCTAGCT
GCTGAAGCAGCAGATAACCTAATCGAACGATTGCCTGATATTGACCAGCA
GCTGATAAGCACCGCTGAAAAGCTATCAGATTTATACGTAATTTGCAATG
AAGATGTAGATACAATGTTGAAAATAAAATATTTAGAAAAATCTTTATCG
TTACAGGAGAAATATAATTTAAACAAATTGAGTTCATATCATTGCCTGTC
CAGAATTTTTTGTAATTTAGGCATTGATTGTTACCTTGATTATATGGAAA
ATAGAGGCCAAAGATCTTTCGATGAAGCTTTGATTGATTTTAAAAAGTCT
ATTAAGTTTTGTAAATTAAAGAAAAGAGAACCGATTTCAGAAAATGCATT
AGAAGTAATTGCTATGAAAGATGCTGTATCTAAAGGAATATCAGATTATG
TTAATATTGAAACTGAAAAATTTCTTGCGATGAGTTACAATAGAATTGGT
GTTTTAACATATGACGTTTTTGTCATGAAAAAATCTGTTAGATATTATAA
TAAATTGTTGGAAATGAATTTGTCGGTTGAAGCTAAAAGCTTTTACGAAA
ATCAAAAAACCAAGTGTGAAACAAGAATTACAAGTGTCATATCCACATTG
GAATTTCTTGTAAGCGATTCTAGAAAGATTTTCGTTACAGATTATTCAAT
AGAGTTTTTGTGTTGGAAATTTCCGAATTTTGTCACCGAAAGCAATTGCC
AAGAGTTATTTAAAAAAACAAATCAAAGTCTCATTGATAATCTTTCACCA
TTTATAAATAGGGAATTAACAAAATTTTCTGAAGAATTCTTTGTTCTTTA
TAAATTTTATAAATTACTTATCATATACGGATTGATTAGAAAATTCCCCG
AATTTCCGTAGATAACTTTGAACAAAATTTATTATGAGTTTATATTACAA
TTATACAGTAAAAATCTGTTTGCGGAATTAGTTGAAATTTATTGAAATCT
GACAACTGAGAGCCACTTAAAATGTTTATTCGATTTAAGTAAAATCGAAA
GTAACATTTTTAATTTGATACAATGATTCTATCAGCGTTAAAGATTAATT
ATTTTCTGCAAATTTGGATC
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protein sequence of SMED30031487-orf-1

>SMED30031487-orf-1 ID=SMED30031487-orf-1|Name=SMED30031487-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1409bp
MIHNVFDREYWYNYFDVDLLLKFECKDQIIVSDAASSDALHQSHIISRVC
KNLLDKDKFIKSAVIPINIGNVVDGIYEGSHWVGLVININPDKSFEALYA
DSFNENVNETLKTVLTNKGINAIKNLSQKQQNNVFDCGPWVVFNLISIAR
TRNLAVGIDEEMILKQRLTLRLLLNDKKDECNTMNISDFPMENSVLINST
NLLKTNQTNELVSNDIDIIYEMKCSIINFLNNKLDLQNCLIEIGNLSEQI
SYNDKNDPQSKSIRNKYGFIQRNKNKQKNINGIYFEKIFQLKIICSDDIV
YSGIAQHEKDKLGRDLINLKILLNKLLLTENRNESINELESIPDGLFFID
NIVEIASKYYDIIIFREIRKTIKNIDNINFSIKEDRYFIARILTVIGQLL
NEYLESKSINIKTNKLFIGFQDLMNIRNLTVHFHAKIKTFADNENQLSIL
NDSIINIRHHLENIITEDFQINSQYDNSENFIESTKIILNYLKFKSKPNS
IVEKNSELIKINIKNLISKSVKIIERMGSKTKDDSQLMKLNNEYQNLINQ
LDAQDKCDYPELLSISNKNILKEFINSMEKNMKGNKSEKAKKVKPIAKNN
KVEMKSVIDDLLWKSYKIVSNNQINSNEIDKQLAPINQHYRSRINQLELS
DRDFYPDEINYSNKNIIIKYNEPKIRSKLLTSIKEINDEMSYLESTENNV
ELIEIKKMYICEHIVLVIGQIVRDLLNLGLCNYQSLNLPDEKWFVSRCIS
SEILSEAGRSARRSRNEGLAHNIWSFDSFKLWKIIINDLLPASNYLEKLF
YINESETLPLSVYSSFTLGILFTDIGLYKRAIEYLQKALDLLNAVSTISD
TSSDYSSVCGMNQFLFQINANLAHVYRLNGQYQNATEILEFLLSKINYHE
AILCENKIIKKEVSHVYVDICYLHILQLNYRKVIEICDQTILISDDLEIG
YYLAIALWEDRQINRAIEIMENLKECNGDTIKSVMFIETIAFMCLDRLEI
EKYFHLVVKCVEEMEKRMENNIMYYKENNPHGYLLEKIVIYYIKCKILME
ESLQKLSYNIDSLKDIIKLSNKTIGKLNFDLKRDRIICYIYICFCKGYLC
YFHFNEGKYHVLLEAEKFFNLAAEAADNLIERLPDIDQQLISTAEKLSDL
YVICNEDVDTMLKIKYLEKSLSLQEKYNLNKLSSYHCLSRIFCNLGIDCY
LDYMENRGQRSFDEALIDFKKSIKFCKLKKREPISENALEVIAMKDAVSK
GISDYVNIETEKFLAMSYNRIGVLTYDVFVMKKSVRYYNKLLEMNLSVEA
KSFYENQKTKCETRITSVISTLEFLVSDSRKIFVTDYSIEFLCWKFPNFV
TESNCQELFKKTNQSLIDNLSPFINRELTKFSEEFFVLYKFYKLLIIYGL
IRKFPEFP*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000099neuron
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR038765Papain-like_cys_pep_sf
IPR011990TPR-like_helical_dom_sf
IPR019734TPR_repeat
IPR003653Peptidase_C48_C
IPR013026TPR-contain_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0008234cysteine-type peptidase activity
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 530..557
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3DG3DSA:1.25.40.10coord: 1040..1301
e-value: 2.2E-5
score: 25.9
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3DG3DSA:1.25.40.10coord: 787..1007
e-value: 6.1E-11
score: 44.2
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILYSSF48452TPR-likecoord: 812..973
IPR019734Tetratricopeptide repeatPROSITEPS50005TPRcoord: 812..845
score: 8.024
IPR019734Tetratricopeptide repeatPROSITEPS50005TPRcoord: 1265..1298
score: 5.694
IPR013026Tetratricopeptide repeat-containing domainPROSITEPS50293TPR_REGIONcoord: 812..845
score: 8.755
IPR003653Ulp1 protease family, C-terminal catalytic domainPROSITEPS50600ULP_PROTEASEcoord: 1..148
score: 10.422
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILYSSF54001Cysteine proteinasescoord: 62..176