Pseudouridine-5'-phosphate glycosidase

Overview
NamePseudouridine-5'-phosphate glycosidase
Smed IDSMED30031474
Length (bp)2511
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Pseudouridine-5'-phosphate glycosidase (SMED30031474) t-SNE clustered cells

Violin plots show distribution of expression levels for Pseudouridine-5'-phosphate glycosidase (SMED30031474) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Pseudouridine-5'-phosphate glycosidase (SMED30031474) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Pseudouridine-5'-phosphate glycosidase (SMED30031474) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30031474

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pigment cellSMED30031474SMESG000061340.1 SmedASXL_012207SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult RNA-sequencing evidence
gutSMED30031474SMESG000061340.1 dd_Smed_v4_5339_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30031474SMESG000061340.1 dd_Smed_v4_5339_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031474SMESG000061340.1 dd_Smed_v4_5339_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031474SMESG000061340.1 SMESG000061339.1 Contig49061newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031474SMESG000061340.1 SMESG000061339.1 Contig49061uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
tail regionSMED30031474SMESG000061340.1 dd_Smed_v6_5339_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of Pseudouridine-5'-phosphate glycosidase vs. Ensembl Celegans
Match: T24C12.3 (pep chromosome:WBcel235:X:2196525:2203961:-1 gene:WBGene00020765.1 transcript:T24C12.3.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T24C12.3)

HSP 1 Score: 276.174 bits (705), Expect = 9.367e-82
Identity = 188/546 (34.43%), Postives = 296/546 (54.21%), Query Frame = 1 
Query:  127 KRCFNGFQELIETSEEISQSILDNKPTVALESTVITHGMPWPVNFETALELESIVKEEGAVPVTIGVLNGKLTAGFSKSELEELAQSVNSSCKPIKISRHNIYNSRNIK-YGGTTASGTMIVAKELXXXXXXXXXXXXVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGV-FVCTLDSKVFPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEFHKLKKTNIITTGKTSFSSKLPIVVIGGCMVDNYMTIKDSSVVNLETIVPRATYDCSKVSTCGGVGRNLAEVFHLLNGTCHFLSVLGKDFHGEFIKTKSSQLNLSRIKYTDNYETGNCTIISCENREILFHIGNIS--------IFESITPDYLKGHLEELKSELKWICLDCNVPDKTMEYVIEYAKTNDIKVFIEPTDIMLVEKPVKVIKRFQS-IFTPIDLISPNVFEFYQMIK 1731
            KR F+ F   ++ S ++ +++   +  VALESTVITHG+P+P N  TA  LE  V+  G+ P TI + +GK+  G    +LE LA S N+    +K+S  +I  +   K  GGTT + TM +A   GI++F TGGIGGVHR  + T D+S DL+EL +TP+CVV SG+KS LDIPK++E+LET  V  +      VFP+FFTR+   K+    +++E V  ++       L  G ++A PIPE    DG+ I +AI ++++ A    +    VTPF+L ++N +T G S+  N+ L++NN  +A +LA +    +   I  +  T+ +++ P VV  G  + ++  I    V +        +Y+   V   GGV RN A+    L     F+S +G D +G F +  S +++++R+K   N  T  CT ++   +      GN+         +   ITP  ++ + + L+S   +I LD N+    M  V+E AK +D +V++EPTDI       KV K F + +   +   SPN  EF +  K
Sbjct:    9 KRSFSKF---LQISPQVKEALASGEGVVALESTVITHGLPYPHNLSTARSLEQKVRSSGSHPATIALFDGKIHVGLDDEKLELLASSQNA----VKVSSRDIAKTLIKKEVGGTTVASTMKIAHAAGISVFATGGIGGVHRGADQTFDVSADLQELSQTPVCVVCSGVKSILDIPKTVEYLETHSVNCIVYGQENVFPSFFTRTSDRKAQFCTESLEEVVHLLKTSKSLGLPYGTILACPIPEKYAADGDAIQKAIDQAVQEAIEQNIASQSVTPFILARVNELTQGASMATNIALLENNASIAGRLAAKLCDRRPLTISQSQPTASTTRKPKVVSIGATIVDFEAITSEDVKD-----DGGSYNGQVVQRMGGVARNHADALARLGCDSVFISAIGDDNNGHFFRQNSHKIDINRVKIISNKPT--CTYLAVNVK------GNVKYGIVTCEPLLSVITPSLVESNEDALESS-DFILLDSNLSVPVMARVLEIAKKHDKQVWLEPTDID------KVKKVFDTGLVGAVTAASPNANEFLKWAK 527
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BLAST of Pseudouridine-5'-phosphate glycosidase vs. Ensembl Fly
Match: CG2794 (gene:FBgn0031265 transcript:FBtr0078067)

HSP 1 Score: 330.487 bits (846), Expect = 1.902e-101
Identity = 203/535 (37.94%), Postives = 310/535 (57.94%), Query Frame = 1 
Query:  121 LFKRCFNGFQELIETSEEISQSILDNKPTVALESTVITHGMPWPVNFETALELESIVKEEGAVPVTIGVLNGKLTAGFSKSELEELAQSVNSSCKPIKISRHNI-YNSRNIKYGGTTASGTMIVAKELXXXXXXXXXXXXVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGVFVCTLDSK--VFPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEFHKLKKTNIITTGKTSFSSKLPIVVIGGCMVDNYMTIKDSSVVNLETIVPRATYDCSKVSTCGGVGRNLAEVFHLLNGTCHFLSVLGKDFHGEFIKTKSSQLNLSRIKYTDNYETGNCTIISCENREILFHIGNISIFESITPDYLKGHLEELKSELKWICLDCNVPDKTMEYVIEYAKTNDIKVFIEPTDIMLVEKPVKVIKRFQSIFTPIDLISPNVFEF 1716
            L +R  +   + I    EI Q++   KP VALEST+ITHGMP P N  TAL +E  V++ GA+P TIG+L+G++  G ++ EL  LA+        IK SR ++ +     + GGTT + TMI+A  +GI++F TGGIGGVHR+   +MD+S DL ELGRTP+ VV SG+KS LDIP++LEFLET+GV V + DS   VFP+F+TR  G   P ++K+ +  AE++    + +++SG+++ VPIPE    D   I +AI ++   A++  ++G  VTPF+L  I  IT G+SL  N+ LIKNN +VAA++A     +  T + +  + +   K P+VV G  ++D    + D      +  +  ATY        GGVGRN+AE  + L G  + +S +G D  G+ +     +     +   DN+ T  C++I  +  +    +GN+ I +SIT + L+ H  +L  E   I +D N+ ++ M  +++ A+ N I VF EPTD+ +  KP K++         I LI PN+ E 
Sbjct:   16 LVRRSLHRKHDHIVLHPEIRQALQLQKPVVALESTIITHGMPMPENVVTALAVEEQVRQNGAIPATIGILDGRIKVGLTREELTSLAEKPRDQV--IKCSRRDLPFVVSRRQSGGTTVAATMIIAHRVGIHVFATGGIGGVHRDGHESMDVSADLTELGRTPVAVVCSGVKSILDIPRTLEFLETQGVCVASFDSPGGVFPDFYTRDSGCTVPYNLKSAQEAAELLRSWRELKMESGLVIGVPIPEEFAADKFKIEEAIKEATAQARAQGISGKEVTPFLLAAIAKITEGRSLKSNIALIKNNAKVAAQIAASLCDV-STKLESPVQKAIDKK-PLVV-GASILDLSFKVDDQ---KRDMKLDGATYSAVAKQAAGGVGRNIAEGIYKLYGDVNLISAVGNDQMGQTLLQMMPKALKRGLIVADNHNTSLCSLIFDKFGDCKLILGNMEIHQSITAETLQAH-HQLFREAPLIIMDSNISEQAMASILQQAQINKIPVFFEPTDMFIAGKPFKLLPELTK---NIRLIKPNMQEL 538
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BLAST of Pseudouridine-5'-phosphate glycosidase vs. Ensembl Zebrafish
Match: zgc:136858 (zgc:136858 [Source:ZFIN;Acc:ZDB-GENE-060421-6632])

HSP 1 Score: 310.457 bits (794), Expect = 8.668e-94
Identity = 209/551 (37.93%), Postives = 303/551 (54.99%), Query Frame = 1 
Query:  121 LFKRCFNGFQE-------LIETSEEISQSILDNKPTVALESTVITHGMPWPVNFETALELESIVKEEGAVPVTIGVLNGKLTAGFSKSELEELAQSVNSSCKPIKISRHNI-YNSRNIKYGGTTASGTMIVAKELXXXXXXXXXXXXVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGVFVCTL-DSKVFPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEFHKLK-------KTNIITTGKTSFSSKLPIVVIGGCMVDNYMTIKDSSVVNLETIVPRATYDCSKVSTCGGVGRNLAEVFHLLNGTCHFLSVLGKDFHGEFIKTKSSQLNLSRIKYTDNYETGNCTIISCENREILFHIGNISIFESITPDYLKGHLEELKSELKWICLDCNVPDKTMEYVIEYAKTNDIKVFIEPTDIMLVEKPVKVIKRFQSIFTPIDLISPNVFEFYQM 1725
              +RCF            L     ++ +++  ++P VALEST+ITHGMP+P N  TA E+E+IV+ EG++P TIG+L G++  G S  EL+ LAQS  +    +K+SR ++ Y       GGTT SGTMI A + GI +F TGGIGGVHR+ E+++D+S DL ELGRTPI VVS+G+KS LDI ++LE+LET+GV V T  DSK FP FF+R  G  SP +I + +  A++I       LKSG+L+AVPIPE     G  I  AI  ++  A S  VTG  VTPF+L +++ +T GKSL  N+ LI+NN RV +++A    KL        + NI T            +VIGG  VD     K ++    + ++   T   S   + GGVGRN+A+    L     F+S +GKD H + +      ++ S +    +  T     +  E+ E+   +G++ I + I   Y+   +++L S    I LD N+P  T+ YV   AK + + V+ EPTD     KP          +  +   SPN+ E   M
Sbjct:    8 FLRRCFTTTHSRHCKQDSLFTIHPDVKEALAHHRPVVALESTIITHGMPYPHNLSTAKEVEAIVRAEGSIPATIGILEGRIHVGLSSDELDFLAQSKTA----LKVSRRDLPYVISKGLSGGTTVSGTMIAANKAGIPVFVTGGIGGVHRDGENSLDVSADLTELGRTPIAVVSAGVKSILDIGRTLEYLETQGVCVATYGDSKSFPAFFSRQSGFTSPYNISSPQEAADLIASTFSLGLKSGLLLAVPIPEEHAATGQQIEDAIQTAVTEASSKGVTGRDVTPFILQRVSELTKGKSLQANIALIRNNARVGSQIAHALSKLNENKEGHVRGNIKTQKTMDQKMDSKTIVIGGINVD--FIAKGTT----KKLLFGQTNPGSVCQSFGGVGRNIADCLSRLGHKPLFISAIGKDSHSDAVLNYCKHMDTSAVARLQDQRTATYCAVITESGELSLGLGDMDIHQQIQEQYVSQFVDQLSSA-SLIVLDGNIPVSTINYVCRIAKKHAVPVWFEPTDADKACKPF-----LSESWKALAFTSPNLTELCTM 542
[Close]
BLAST of Pseudouridine-5'-phosphate glycosidase vs. Ensembl Xenopus
Match: ENSXETT00000005835.1 (pseudouridine-metabolizing bifunctional protein C1861.05-like [Source:NCBI gene;Acc:100485794])

HSP 1 Score: 303.138 bits (775), Expect = 1.011e-90
Identity = 200/532 (37.59%), Postives = 293/532 (55.08%), Query Frame = 1 
Query:  172 EISQSILDNKPTVALESTVITHGMPWPVNFETALELESIVKEEGAVPVTIGVLNGKLTAGFSKSELEELAQSVNSSCKPIKISRHNI-YNSRNIKYGGTTASGTMIVAKELXXXXXXXXXXXXVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGVFVCTL-DSKVFPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEFHKL---KKTNIITTGKTSFSSKLPI----VVIGGCMVDNYMTIKDSSVVNLETIVPRATYDCSKVSTCGGVGRNLAEVFHLLNGTCHFLSVLGKDFHGEFIKTKSSQLNLSRIKYTDNYETGN-CTIISCENREILFHIGNISIFESITPDYLKGHLEELKSELKWICLDCNVPDKTMEYVIEYAKTNDIKVFIEPTDIMLVEKPVKVIKRFQSIFTPIDLISPNVFEFYQMIKSF 1737
             + +++   +P VALEST+ITHGMP+P N   A E+E IVK  G+VP T+G+L G+L  G    EL+ LAQS +     +K+SR ++ Y       GG+T SGTMI A   GI +F TGG+GGVH E E TMD+S DL ELGRTP+ V+S+G+KS LDI ++LE+LETEGV V T  DS  FP FFT   G K+P  ++     A++I    +  L SG+L+AVPIP      GN I +A+ ++L  A+   V G  VTPF+L ++N +T G+SL  N+ LIKNN RV +++A    +L   K       G     S+ P+    VVIGG  VD        S+V      P   +      + GGVGRNLA+    L     F+S LG+D H   +    S +++  +     + T   C +IS    E+   +G++ I + IT +Y+     +  ++ + +C+D N+P  T++YV   ++ + + V  EPTD     KP          +T +  ISPN+ E   + ++ 
Sbjct:   40 RVQEALATGEPVVALESTIITHGMPYPYNIRMAKEVEEIVKANGSVPATVGILQGRLHVGLCDEELQFLAQSKDC----VKVSRRDLPYVLSQGLSGGSTVSGTMIAAHRAGIPVFVTGGVGGVHHEGEKTMDVSADLTELGRTPVAVISAGVKSILDIGRTLEYLETEGVCVSTFGDSTDFPAFFTPKSGFKAPCHVRDEVEAAKLIARSLELGLSSGILLAVPIPAEHAAPGNAIEEAVQQALREARLQGVQGKEVTPFLLQRVNELTGGQSLESNIALIKNNARVGSRIAVALSQLRNPKGGQRKKPGTPGTESEPPMDSRPVVIGGINVDFIAKAAHDSMVFGGQTNPGQVHQ-----SFGGVGRNLADCLCRLGAKPLFISALGQDDHANSVMHYCSHMDMGGVARLKGHNTATYCAVISGTG-ELSLGLGDMDIHQQITENYVS-QFADCLAQARLVCIDGNIPLSTIQYVCGLSRRHGVPVCFEPTDEDKSCKPF-----LSDSWTALSFISPNLKELIGINRAL 555
[Close]
BLAST of Pseudouridine-5'-phosphate glycosidase vs. Ensembl Xenopus
Match: ENSXETT00000005726.1 (pseudouridine-metabolizing bifunctional protein C1861.05-like [Source:NCBI gene;Acc:100485794])

HSP 1 Score: 207.994 bits (528), Expect = 1.196e-58
Identity = 132/389 (33.93%), Postives = 204/389 (52.44%), Query Frame = 1 
Query:  559 TMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGVFVCTL-DSKVFPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEFHKLKKTNIITTGKTSFSSKL-PIVVIGGCMVDNYMTIKDSSVVNLETIVPRATYDCSKVSTCGGVGRNLAEVFHLLNGTCHFLSVLGKDFHGEFIKTKSSQLNLSRIKYTDNYETGN-CTIISCENREILFHIGNISIFESITPDYLKGHLEELKSELKWICLDCNVPDKTMEYVIEYAKTNDIKVFIEPTDIMLVEKPVKVIKRFQSIFTPIDLISPNVFEF 1716
            TMD+S DL ELGRTP+ V+S+G+KS LDI ++LE+LETEGV V T  DS  FP FFT   G K+P  ++     A++I    +  L SG+L+AVPIP      GN I +A+ ++L  A+   V G  VTPF+L ++N +T G+SL  N+ LIKNN RV +++A    +L+       G+     +L P++   GC           S++         T       + GGVGRNLA+    L     F+S LG+D H   +    S +++  +     + T   C +IS    E+   +G++ I + IT +Y+     +  ++ + +C+D N+P  T++YV   ++ + + V  EPTD     KP          +T +  ISPN+ E 
Sbjct:   27 TMDVSADLTELGRTPVAVISAGVKSILDIGRTLEYLETEGVCVSTFGDSTDFPAFFTPKSGFKAPCHVRDEVEAAKLIARSLELGLSSGILLAVPIPAEHAAPGNAIEEAVQQALREARLQGVQGKEVTPFLLQRVNELTGGQSLESNIALIKNNARVGSRIAVALSQLRNPK---GGQRKKPGRLTPLM---GC-----------SILLPPLQFGGQTNPGQVHQSFGGVGRNLADCLCRLGAKPLFISALGQDDHANSVMHYCSHMDMGGVARLKGHNTATYCAVIS-GTGELSLGLGDMDIHQQITENYVS-QFADCLAQARLVCIDGNIPLSTIQYVCGLSRRHGVPVCFEPTDEDKSCKPF-----LSDSWTALSFISPNLKEL 391
[Close]
BLAST of Pseudouridine-5'-phosphate glycosidase vs. UniProt/SwissProt
Match: sp|Q9USY1|YOW5_SCHPO (Pseudouridine-metabolizing bifunctional protein C1861.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC1861.05 PE=3 SV=1)

HSP 1 Score: 245.743 bits (626), Expect = 9.592e-69
Identity = 216/677 (31.91%), Postives = 345/677 (50.96%), Query Frame = 1 
Query:   82 MLNKLIKFTSK-PLLFKRCFN----GFQELI-------ETSEEISQSILDNKPTVALESTVITHGMPWPVNFETALELESIVKEEGAVPVTIGVLNGKLTAGFSKSELEELAQSVNSSCKPIKISRHNI-YNSRNIKYGGTTASGTMIVAKELXXXXXXXXXXXXVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGVFVCTLD--SKVFPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEFHKLKK------TNIITT------------GKTS--------------FSSKLPIVVIGGCMVDNYMTIKDSSVVNLETIVPRATYDCSKVSTCGGVGRNLAEVFHLLNGTCHFLSVLG-KDFHGEFIKTKSSQLNLSR-IKYTDNYETGNCTIISCENREILFHIGNISIFESITPDYLKGHLEELKSELKWICLDCNVPDKTMEYVIEYAKTNDIKVFIEPTDIMLVEKPVKVIKRFQSIFTPIDLISPNVFE---FYQMIK--SFPESDS-YSDLMEFDSKSFEFNQTKIF------EXXXXXXXXXXXXXXIP---KWIIKLGPAGVVFASKTEM 1920
            ++N+  +  S+ PL F+R       G + ++         S+E+++++ +N P VALEST+ITHGMP+P N E A+++ES V+  GAVP TI +LNG+ T G  + +L ELA+S  ++    K+SR ++ Y +     GGTT + TMI+A+  GI++F TGGIGGVHR  E++MDIS DL ELGRT + VVS+G+KS LDI ++LE LET+GV V TL      FP FF+R    +SP  ++T + +A ++F   +   + G LVA+P P     D   +   I   L+ +    +TG  VTP++L ++   + GKSL  N++L+ NN   A+ +AKE   LK+      TN   T            GK S               S    +V +G   +D+ + + +     L +     ++ C      GGV  N+A    L+  +   +S +G K      IK   ++ +L   I    N+ + + T I+  N  +L    +++I E+++   +K  L    ++ K+IC D N+    M   I  +K++  +V  EPT      K +K++K        ID I+PN FE    +Q +K  +F E++S +  L  F   S  +N+ + F      E +           L+P     I+KLGP G +  S  ++
Sbjct:    4 VMNRGCRLVSRIPLGFRRLKRNPNPGLRNILCRRYSPLALSKEVTEALKNNVPVVALESTIITHGMPYPQNEELAIQVESKVRSMGAVPATIALLNGQCTIGLEQFQLSELAKSGETA---YKVSRRDLSYVASQRLNGGTTVAATMILARAAGIDVFATGGIGGVHRGAENSMDISADLIELGRTRVAVVSAGVKSILDIGRTLEVLETQGVPVVTLGPPKSAFPAFFSRESKFQSPLSLETPQLIANMLFSNIQLGQECGTLVAIPTPHHCSIDYEKMEALIETCLQRSVQLGITGKNVTPWLLGELLRESKGKSLNTNIDLVLNNAEKASLIAKELAVLKEKSSFFPTNTGNTFETKPVKQDFFYGKVSDKGVSSSKKKITETTSKPAEVVCVGSVSIDSVLKLDNP----LTSKFLGTSHPCHSEQAFGGVAHNMALASSLMGASTKLVSCVGTKSVPTSSIKEYLTKSSLQHTIVEKSNFTSCSYTAINDCNGNLLLAGADMAIMENLSYSEIKDDL----NDAKYICFDGNISPSLM-LDITTSKSSKQRVVFEPTS---GPKTLKILKVLS--VASIDFITPNKFELDVLFQAMKDGNFFENESWWQKLNSFGITSSFYNEIERFTKSTGIEEITENGILQKCFHLLPFIKNIIVKLGPNGALLISSEKL 663
[Close]
BLAST of Pseudouridine-5'-phosphate glycosidase vs. UniProt/SwissProt
Match: sp|Q1M4T3|PSUG2_RHIL3 (Pseudouridine-5'-phosphate glycosidase 2 OS=Rhizobium leguminosarum bv. viciae (strain 3841) OX=216596 GN=psuG2 PE=3 SV=1)

HSP 1 Score: 231.876 bits (590), Expect = 3.075e-68
Identity = 133/306 (43.46%), Postives = 193/306 (63.07%), Query Frame = 1 
Query:  166 SEEISQSILDNKPTVALESTVITHGMPWPVNFETALELESIVKEEGAVPVTIGVLNGKLTAGFSKSELEELAQS---VNSSCKPIKISRHNIYNSRNIKYGGTTASGTMIVAKELXXXXXXXXXXXXVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGVFVCTLDSKVFPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEFHKLKKTN 1074
            S E++++I    P VALEST+ITHGMP+P N ETAL +E++++E GA+P TI V+ G+L  G    ELEELAQS   V +S + + ++     ++      GTT S TM++A   GI++F TGG+GGVHR  E T DIS DL ELGRT   VV +G+KS LDI K+LE+LET+ V V    ++ FP FFTR  G K+ + + T E +A+ ++   +    +G+L+A PIPE      + I   I  ++ +A    +    +TPF+L +IN +T G+SL  N+EL+KNN R+AA++A  +  LKK  
Sbjct:    9 SREMAEAIAAGSPVVALESTIITHGMPYPANLETALGVETVIRENGAIPATIAVVKGELRVGLEHDELEELAQSKGIVKASGRDLAVAMIRGQSA------GTTVSATMLMADLAGIDVFATGGVGGVHRGAEQTFDISADLTELGRTKTAVVCAGVKSILDIAKTLEYLETQRVPVIAYGTEDFPAFFTRRSGFKADHRLDTPEEIAKAMWLHHQLGTGTGLLIANPIPEASALAPDFIDGTIADAVRDADERGIDRKELTPFLLARINELTKGESLKANIELVKNNARLAARIAVAYAPLKKAR 308
[Close]
BLAST of Pseudouridine-5'-phosphate glycosidase vs. UniProt/SwissProt
Match: sp|A7GCK7|PSUG_CLOBL (Pseudouridine-5'-phosphate glycosidase OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) OX=441772 GN=psuG PE=3 SV=1)

HSP 1 Score: 227.639 bits (579), Expect = 1.069e-66
Identity = 138/309 (44.66%), Postives = 196/309 (63.43%), Query Frame = 1 
Query:  145 FQELIETSEEISQSILDNKPTVALESTVITHGMPWPVNFETALELESIVKEEGAVPVTIGVLNGKLTAGFSKSELEELAQSVNSSCKPIKISRHNIYNSRNIKY-GGTTASGTMIVAKELXXXXXXXXXXXXVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGVFVCTLDSKVFPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEFHKLKK 1068
             ++ +E S+E+S+++ +NKP VALEST+I+HGMP+P N ETAL +E I++++GA+P TI +LNGKL  G +K E+E L +      + +K SR +I      K  G TT + TMIVA   GI +FGTGGIGGVHR  + + DIS DL+EL  T + VV +G KS LDI  +LE+LET+GV V    ++  P F+TR  G K    + T + +AE +       LK GM+V  PIPE  + D + I +AI  +++ A+   + G   TPF+L ++  IT GKSL  N++L+ NNV VA+ LA E  KL K
Sbjct:    2 LEKYLEISKEVSEALKENKPVVALESTIISHGMPYPKNAETALNVEKIIRDKGAIPATIAILNGKLKVGLTKDEIEYLGKK---GKEVVKTSRRDIPFILAKKLDGATTVASTMIVANLAGIKVFGTGGIGGVHRGAQESFDISADLQELANTNVAVVCAGAKSILDIGLTLEYLETQGVPVVGFGTEELPAFYTRKSGFKVDYRVDTAKELAEALKAKWDLGLKGGMVVGNPIPEEYQMDYDTITKAINDAVKEAEEKGIKGKESTPFLLAKVKDITKGKSLEANIQLVYNNVAVASDLAIELSKLNK 307
[Close]
BLAST of Pseudouridine-5'-phosphate glycosidase vs. UniProt/SwissProt
Match: sp|C0ZIY1|PSUG_BREBN (Pseudouridine-5'-phosphate glycosidase OS=Brevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599) OX=358681 GN=psuG PE=3 SV=1)

HSP 1 Score: 227.254 bits (578), Expect = 1.643e-66
Identity = 133/299 (44.48%), Postives = 189/299 (63.21%), Query Frame = 1 
Query:  166 SEEISQSILDNKPTVALESTVITHGMPWPVNFETALELESIVKEEGAVPVTIGVLNGKLTAGFSKSELEELAQSVNSSCKPIKISRHNI-YNSRNIKYGGTTASGTMIVAKELXXXXXXXXXXXXVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGVFVCTLDSKVFPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEFHK 1059
            +EE+  ++ +N P VALE+T+I+HGMP+P N E A E+E I+++ GAVP TIG+++GK+  G + SELEE A + N +    K+SR +  Y   + K G TT + TMI A+  GI++F TGGIGGVHRE E T D+S DL EL +T + VV +G KS LDI ++LE+LET+GV V    +  FP+FF R  G      I T E V +++       LK GM++A P+PE    +   I   I K+L  AK   + G  VTPF+L+++  +T GKSL  N+ L+K+N  VAAK+A  + K
Sbjct:    8 TEEVRHALENNLPVVALETTIISHGMPYPQNIEMAKEVEQIIRDNGAVPATIGIMDGKIKIGLTDSELEEFATNKNVA----KVSRRDFAYILASGKIGATTVAATMIAAEMAGIHMFATGGIGGVHREGEITWDVSADLTELAQTDVAVVCAGAKSILDIGRTLEYLETQGVPVVGYRTDEFPSFFARKSGFGVDMRIDTPEEVGKMMNTKWDLGLKGGMIIANPVPESDALNHEEIEAVIQKALAEAKENNIAGKQVTPFMLDKVKKLTEGKSLATNIALVKHNAEVAAKIAVAYQK 302
[Close]
BLAST of Pseudouridine-5'-phosphate glycosidase vs. UniProt/SwissProt
Match: sp|A6TL60|PSUG_ALKMQ (Pseudouridine-5'-phosphate glycosidase OS=Alkaliphilus metalliredigens (strain QYMF) OX=293826 GN=psuG PE=3 SV=1)

HSP 1 Score: 215.698 bits (548), Expect = 2.558e-62
Identity = 129/309 (41.75%), Postives = 189/309 (61.17%), Query Frame = 1 
Query:  148 QELIETSEEISQSILDNKPTVALESTVITHGMPWPVNFETALELESIVKEEGAVPVTIGVLNGKLTAGFSKSELEELAQSVNSSCKPIKISRHN----IYNSRNIKYGGTTASGTMIVAKELXXXXXXXXXXXXVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGVFVCTLDSKVFPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEFHKL 1062
            ++ +E + E+ ++I + +P VALEST+I+HGMP+P N ETA ++E +++E GAVP TI ++ GK+  G ++ EL  L QS +     +K SR +    I    N   G TT + TMI+A   GI +F TGGIGGVHR  + T+DIS DL EL +T + VV +G+KS LDI  +LE LET GV +    ++ FP F+TR  G      +  +E +A  +       LK G++VA PIPE    D  +I QAI ++++ A +  + G   TPF+L +I  IT GKSL  N+EL+ NN ++ A+LA   H+L
Sbjct:    5 EKYLEYAPEVKKAIAEGRPIVALESTIISHGMPYPQNVETAKKVEDMIREGGAVPATIAIVKGKIKVGLTEEELMFLGQSKDV----VKASRRDLPLIIAKELN---GATTVASTMIIAALAGIKVFVTGGIGGVHRGAQETLDISADLMELAQTNVAVVCAGVKSILDIGLTLEVLETHGVPIVGYQTEDFPAFYTRKSGYGVDYAVANVEELARALKAKWDLDLKGGVVVANPIPEAYAMDEALITQAIDEAVQEAAAKGIQGKETTPFLLTKIKEITEGKSLESNIELVFNNAKIGAELAVALHRL 306
[Close]
BLAST of Pseudouridine-5'-phosphate glycosidase vs. TrEMBL
Match: A0A0L0BXN5 (PfkB domain-containing protein OS=Lucilia cuprina OX=7375 GN=FF38_04844 PE=3 SV=1)

HSP 1 Score: 355.525 bits (911), Expect = 2.012e-107
Identity = 221/547 (40.40%), Postives = 328/547 (59.96%), Query Frame = 1 
Query:  106 TSKPLLFKRCFNGFQELIETSEEISQSILDNKPTVALESTVITHGMPWPVNFETALELESIVKEEGAVPVTIGVLNGKLTAGFSKSELEELAQSVNSSCKPIKISRHNI-YNSRNIKYGGTTASGTMIVAKELXXXXXXXXXXXXVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGVFVCTLDSK--VFPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEFHKLKKTNIITTGKTSFSSKLPIVVIGGCMVDNYMTIKDSSVVNLETIVPRATYDCSKVSTCGGVGRNLAEVFHLLNGTCHFLSVLGKDFHGEFIKTKSSQLNLSRIKYTDNYETGNCTIISCENREILFHIGNISIFESITPDYLKGHLEELKSELKWICLDCNVPDKTMEYVIEYAKTNDIKVFIEPTDIMLVEKPVKVIKRFQSIFTPIDLISPNVFEFYQMIKSF 1737
            T K L F+R  + F++L++    +  ++   +P VALEST+ITHGMP+P N ETA E+E IV+  GA P TI +++G+L  G SK ++ +LA+   S    IK SR ++ Y   N K GGTT + TMIVA   GI IF TGG+GGVHR+   TMD+S DL ELGRTP+ VVSSG+KS LDIP++LE+LET+GV V   +S+   FP+F+TR  G K+P +++     A++I  + + QL+SG+L+ VPIP     D ++I  AI ++ ++A +  V G  VTPF+L  I  IT G SL  N+ LIKNN  VAAK+A E   +   +  T+  TS +S+ P+ VIG  M+D  +T+ D   +NL+     ATY  S   + GGVGRNLAE  + LNG+  F+SV+GKD  G+ +     +     I       T  C+II  +       + N+ I +S+TP  ++   E+ K     + +D N+   T+E ++E +   +  VF EPTD+++  KP ++     ++   I LI+PNVFE   ++++ 
Sbjct:    8 TIKILSFQRNLSSFRKLLDIHPTVKNALYHQEPVVALESTIITHGMPYPQNLETAFEVEDIVRSSGATPATIALMDGRLKVGLSKDDIVQLAKMDRSEV--IKSSRRDLAYVISNGKCGGTTVAATMIVAHMAGIKIFATGGVGGVHRDGHITMDVSADLIELGRTPVTVVSSGVKSILDIPRTLEYLETQGVCVAAFNSEGGKFPDFYTRDSGCKAPYNLQNPLEAAKLINSLHQLQLQSGILIGVPIPAEYAADKDLITAAIDQAYKDAAAQGVEGKEVTPFLLAAIAKITGGSSLKANMALIKNNAHVAAKIACELSNIVNKSTKTSEHTSTTSRNPL-VIGASMLDLCLTMLDDIPLNLDG----ATYRTSAKESGGGVGRNLAEAIYKLNGSASFVSVIGKDHMGQSLLQLIPESMRQGILIDQEKTTSICSIIFDKFGNCKMCLANMEIHKSLTPKVIRQQEEQFK-HTPLVVIDSNLSVNTIETILELSLQYNKPVFFEPTDMLIAGKPFELDT---TLTQQIRLITPNVFELKAIVEAI 543
[Close]
BLAST of Pseudouridine-5'-phosphate glycosidase vs. TrEMBL
Match: A0A0M4E876 (CG2794 OS=Drosophila busckii OX=30019 GN=Dbus_chr2Lg249 PE=3 SV=1)

HSP 1 Score: 345.895 bits (886), Expect = 8.353e-104
Identity = 212/541 (39.19%), Postives = 313/541 (57.86%), Query Frame = 1 
Query:  124 FKRCFNGFQELIETSEEISQSILDNKPTVALESTVITHGMPWPVNFETALELESIVKEEGAVPVTIGVLNGKLTAGFSKSELEELAQSVNSSCKPIKISRHNI-YNSRNIKYGGTTASGTMIVAKELXXXXXXXXXXXXVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGVFVCTLDSK--VFPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEFHKLKKTNIITTGKTSFSSKLPIVVIGGCMVDNYMTIKDSSVVNLETIVPRATYDCSKVSTCGGVGRNLAEVFHLLNGTCHFLSVLGKDFHGEFIKTKSSQLNLSRIKYTDNYETGNCTIISCENREILFHIGNISIFESITPDYLKGHLEELKSELKWICLDCNVPDKTMEYVIEYAKTNDIKVFIEPTDIMLVEKPVKVIKRFQSIFTPIDLISPNVFEFYQMIKSF 1737
             +R F   Q  I    EI  ++    P VALEST+ITHGMP P N ETAL++E  V+ +GA+P TIG+++G +  G +K EL ELA         IK SR ++ Y     + GGTT + TM++A+ +GI IF TGGIGGVHRE   T+DIS DL ELGRTP+ VV SG+KS LDIP++LE LET+GV V + DS   VFP+F+TR+ G K P ++      AE++      QL+SG+L+ VPIP     D + I  AI ++   A++  ++G  VTPF+L +I  +T G+SL  N+ LIKNN +VAA++A    K+++     T   + S+K   VV+G  ++D    +K+S  + L+     ATY      T GGVGRNLAE  + L G+C  +S +G D  G+ ++    Q         D+  T  C++I     +    +GN+ +  SITP+ L    +EL S+   + +D N+ +++M  ++E A+   + VF EPTD+ +  KP ++     ++   I LISPN+ E   +  S 
Sbjct:   14 IRRAFATKQAQILVHPEIKYALEQKHPIVALESTIITHGMPLPENVETALQVEQQVRSQGALPATIGIIDGCIKVGLTKEELTELAHKPREHV--IKCSRRDLPYVVAKAQSGGTTVATTMLIAERVGIKIFATGGIGGVHREGHVTLDISADLVELGRTPVAVVCSGVKSILDIPRTLEVLETQGVCVASYDSPGGVFPDFYTRNSGCKVPYELANPTEAAELLQAWHALQLRSGVLIGVPIPAEYAADKHAIEAAIKEANYEAQAQGISGKEVTPFLLARIAKLTKGRSLQSNIALIKNNAKVAAEIACALAKIQQKGPGKTANRTTSTKSQPVVVGASILDLSFKLKESRELQLDG----ATYSSVTQQTPGGVGRNLAEGIYKLYGSCQLISAVGNDQMGQMLQQLMPQALQRSFIVDDSSSTSLCSVIFDHYGDCKLILGNMEVHASITPEQLLAR-QELISQAPLLIMDSNISEQSMACLLELAQRYKLPVFFEPTDMFIAGKPFQLQP---ALTQQIRLISPNLQELRTIAASI 544
[Close]
BLAST of Pseudouridine-5'-phosphate glycosidase vs. TrEMBL
Match: A0A210PPV3 (PfkB domain-containing protein OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT23992 PE=3 SV=1)

HSP 1 Score: 344.354 bits (882), Expect = 7.066e-103
Identity = 215/554 (38.81%), Postives = 316/554 (57.04%), Query Frame = 1 
Query:  154 LIETSEEISQSILDNKPTVALESTVITHGMPWPVNFETALELESIVKEEGAVPVTIGVLNGKLTAGFSKSELEELAQSVNSSCKPIKISRHN----IYNSRNIKYGGTTASGTMIVAKELXXXXXXXXXXXXVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGVFVCTLD-SKVFPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEFHKLKK----------TNIITTGKTSFSSKLP----------IVVIGGCMVDNYMTIKDSSVVNLETIVPR-ATYDCSKVSTCGGVGRNLAEVFHLLNGTCHFLSVLGKDFHGEFIKTKSSQLNLSRIKYTDNYETGNCTIISCENREILFHIGNISIFESITPDYLKGHLEELKSELKWICLDCNVPDKTMEYVIEYAKTNDIKVFIEPTDIMLVEKPVKV-IKRFQSIFTPIDLISPNVFEFYQMIKS 1734
            +++ SEE+  ++   KP VALEST+ITHGMP+P N  TA E+E+IV+E  ++P TIG+L GK+  G S  ELE LA    S    IK SR +    I   R+   GGTT SGTM+ A   GI++F TGGIGGVHR VE+TMD+S DL ELGRTP+ VVSSG+KS LDI ++LEFLET+GV V +   S+ FP FF+   G  +P +++     A +I    +  L+SG+L+AVPIPE     G ++  AI  ++  A  A ++G  VTPF+L Q+N IT G SL+ N+ LI+NN RV +++AK+    +K          ++ +TT +    S+ P          +VVIGG  VD Y      ++VN +T      TY      + GGVGRNL +    L     F+S +G D H    +  SS ++LS +   D+  T     +     E++F IG++ I +++T + + G  EEL S    +C+D N+P +T+++V +      + V+ EPTD+    KP    +K+  +       +SPN+ E  QM ++
Sbjct:   31 IVQLSEEVKSAVHYGKPVVALESTIITHGMPYPENLRTAQEVETIVRENDSIPATIGILAGKIKVGLSAEELEYLATPSRSV---IKTSRQDLPIVISEGRD---GGTTVSGTMVAANMAGISVFVTGGIGGVHRGVEATMDVSADLTELGRTPVTVVSSGVKSILDIARTLEFLETQGVCVASYGPSRDFPAFFSPRSGFLAPYNVEDPSRAARLIDAHHRLGLESGVLIAVPIPEDQAGQGKLLEDAIQIAVAKASEAGISGKEVTPFILQQVNEITKGASLVANIALIRNNARVGSQIAKQLSIYRKSSPDGATNTESDDVTTVRPHIKSRSPAQQSVKGKGRVVVIGGINVDFY------AMVNKDTFATDGGTYPGGVRQSFGGVGRNLGDCLSRLGVNPLFISAVGNDSHKPAFQHYSSHMDLSGVAVRDDQTTATYCAVLKRTGELIFGIGDMDIHQTVTSEMVSGFEEELASA-PLVCVDGNIPAETIQHVCQLCSKASVPVWFEPTDVHKAGKPFGAGVKKLPT------YVSPNLHELQQMYQA 565
[Close]
BLAST of Pseudouridine-5'-phosphate glycosidase vs. TrEMBL
Match: W8C0I5 (Pseudouridine-metabolizing bifunctional protein C1861.05 OS=Ceratitis capitata OX=7213 GN=YOW5 PE=2 SV=1)

HSP 1 Score: 338.961 bits (868), Expect = 4.756e-101
Identity = 214/555 (38.56%), Postives = 327/555 (58.92%), Query Frame = 1 
Query:   85 LNKLIKFTSKPLLFKRCFNGFQELIETSEEISQSILDNKPTVALESTVITHGMPWPVNFETALELESIVKEEGAVPVTIGVLNGKLTAGFSKSELEELAQSVNSSCKPIKISRHNI-YNSRNIKYGGTTASGTMIVAKELXXXXXXXXXXXXVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGVFVCTLDSKVF--PNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEFHKLKKTNIITTGKTSFSSKLPIVVIGGCMVDNYMTIKDSSVVNLETIVPRATYDCSKVSTCGGVGRNLAEVFHLLNGTCHFLSVLGKDFHGE-FIKTKSSQLNLSRIKYTDNYETGNCTIISCENREILFHIGNISIFESITPDYLKGHLEELKSELKWICLDCNVPDKTMEYVIEYAKTNDIKVFIEPTDIMLVEKPVKVIKRFQSIFTPIDLISPNVFEFYQMIKSF 1737
            + KL+  +SK +L      GF+ LI+    + +S+      VALEST+ITHGMP+P N ETA  +E +V E GA+  TI ++NG++  G +  E+++LA   ++    IK SR ++ Y       GGTT + TMI+A   GI+IF TGG+GGVHR+   TMD+S DL ELGRTPI VVSSGIKS LDIP++LE+LET+GV V T     F  P+F+TR+ G K P ++   +  A +I  + + Q +SG+L+ VP+PE    + N I  AI +++  A++  ++G  VTP++L  I  +T G SL  N+ LIKNN + AA++++E  K KK+ + +  K   SSK P+ VIG  ++D  +T+ D    +L  ++  ATY      + GGVGRN+AE    L G+ +F+SV+G D  G   +K    QL  S +K      T  CTII  ++ +   ++ N+ I ++ITP+ ++   EEL      + +D N+  +TME  +  A      VF EPTD+ + +KP  + K   ++   I  ISPN++E   ++++ 
Sbjct:    6 VKKLVSISSKCVLLN-PLRGFKRLIDIHPNVQRSLQQGDAVVALESTIITHGMPYPENIETAQAVEQLVSENGALAATIAIINGRIKVGLTTDEMKQLAAKNHNEV--IKCSRRDLPYLVSTQGCGGTTVAATMIIAHMSGIDIFATGGVGGVHRDGHITMDVSADLVELGRTPITVVSSGIKSILDIPRTLEYLETQGVCVATYGVPDFNFPDFYTRNSGCKVPYNLNGPKEAANLIKALKELQYRSGILIGVPVPEQFAANKNEIEAAIQEAIYEARNQGISGKQVTPYLLAAIAKLTKGSSLEANMALIKNNAKTAAQISRELSKTKKSYVFSGEKK--SSKTPL-VIGASILDLSLTMLD----DLPKVLDGATYRAKPTESAGGVGRNIAEGISKLYGSANFISVVGNDQLGRSLLKMIPGQLK-SLVKIDGESSTSLCTIIFDKSGDCKIYLANMEIHDNITPETIQ-QSEELLRSAPIVLMDGNLSLETMERTLRLAHKYQRPVFFEPTDMRIAQKPFNLAK---TLTKQIKFISPNIYELRTIVEAI 545
[Close]
BLAST of Pseudouridine-5'-phosphate glycosidase vs. TrEMBL
Match: A0A4D9E773 (Protein brambleberry-like OS=Platysternon megacephalum OX=55544 GN=DR999_PMT14812 PE=3 SV=1)

HSP 1 Score: 337.421 bits (864), Expect = 5.112e-100
Identity = 213/533 (39.96%), Postives = 305/533 (57.22%), Query Frame = 1 
Query:  166 SEEISQSILDNKPTVALESTVITHGMPWPVNFETALELESIVKEEGAVPVTIGVLNGKLTAGFSKSELEELAQSVNSSCKPIKISRHNI-YNSRNIKYGGTTASGTMIVAKELXXXXXXXXXXXXVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGVFVCTL-DSKVFPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEFHKLKKTNIITTGKTSFSSK-----LPIVVIGGCMVDNYMTIKDSSVVNLETIVPRATYDCSKVSTC-GGVGRNLAEVFHLLNGTCHFLSVLGKDFHGEFIKTKSSQLNLSRIKYTDNYETGN-CTIISCENREILFHIGNISIFESITPDYLKGHLEELKSELKWICLDCNVPDKTMEYVIEYAKTNDIKVFIEPTDIMLVEKPVKVIKRFQSIFTPIDLISPNVFEFYQMIKSF 1737
            S  + +++ D +P VALEST+ITHGMP+P N   A E+E IV+  G+VP T+G+LNG++  G    EL+ LA+S N     +K+SR ++ Y       GGTT SGTMIVA + GI +F TGGIGGVHRE E TMD+S DL ELGRT + VVS+G+KS LDI ++LE+LET+GV V    DS+ FP FF+R  G ++P  ++  E  A++I       L SG+L+AVP P+     G +I  AI ++L NA+S  +TG  +TPF+L ++N +T+GKSL  N+ LI+NN RV +++A    K++      TGK S   +     L  VVIGG  VD     +++      TI      +  +VS   GGVGRNLA+    L  T  F+S LGKD H E        +++SR+     + T   C +I+    E+   +G++ I + IT  Y+    E L      +C+D NVP  T++YV E A+ + I V  EPTD     KP          +  +  ISPN+ E   + ++ 
Sbjct:   85 SSSVEEALADGRPIVALESTIITHGMPYPQNLSMAREVEEIVRTNGSVPATVGILNGRIHIGLHDEELQFLAKSKNL----VKVSRRDLPYVLSQGLSGGTTVSGTMIVAHKAGIPVFVTGGIGGVHREGEKTMDVSADLTELGRTAVAVVSAGVKSILDIGRTLEYLETQGVCVAAFGDSREFPAFFSRQSGFQAPCQVQNEEEAAKLIASTLALGLGSGVLIAVPCPKEEAASGQVIEDAIQEALSNARSKGITGKELTPFMLQKVNELTSGKSLDSNIALIQNNARVGSRIAVALSKIQT----ATGKGSLPRREKVTALRPVVIGGINVDFIAKARNT------TIQAGGQTNAGQVSQSFGGVGRNLADCLSRLGKTPLFISALGKDEHSESTLRYCRHMDMSRVLQLAGHNTATYCAVITGSG-ELSLGLGDMDIHQQITEQYVSRFKESL-CLAPLVCIDGNVPVATIQYVCEIAREHQIAVCYEPTDENKASKPF-----LSDSWKALTYISPNLRELRAINRTL 596
[Close]
BLAST of Pseudouridine-5'-phosphate glycosidase vs. Ensembl Cavefish
Match: zgc:136858 (zgc:136858 [Source:ZFIN;Acc:ZDB-GENE-060421-6632])

HSP 1 Score: 296.204 bits (757), Expect = 8.253e-89
Identity = 202/554 (36.46%), Postives = 302/554 (54.51%), Query Frame = 1 
Query:  145 FQELIETSEEISQSILDNKPTVALESTVITHGMPWPVNFETALELESIVKEEGAVPVTIGVLNGKLTAGFSKSELEELAQSVNSSCK-PIKISRHNI-YNSRNIKYGGTTASGTMIVAKELXXXXXXXXXXXXVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGVFVCTLD-SKVFPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEF-HKLKKTNIITTGKTSFSSKLPIVVIGGCMVDNYMTIKDSSVVNLETIVPRATYDCSKVSTCGGVGRNLAEVFHLLNGTCHFLSVLGKDFHGEFIKTKSSQLNLSRIKYTDNYETGN-CTIISCENREILFHIGNISIFESITPDYLKGHLEELKSELKWICLDCNVPDKTMEYVIEYAKTNDIKVFIEPTDIMLVEKPVKVIKRFQSIFTPIDLISPNVFEFYQMIKSF----PESDSYSDLMEFDS 1779
            + +L +    + +++ +++P VALEST+ITHGMP+P N  TA E+E+IV+ +G  P T+G+L+G +  G S  EL+ +     S CK  +K+SR ++ Y       GGTT SGTMI A   GI++F TGGIGGVHR+ E+++D+S DL ELGRTPI VVS+G+KS LDI ++LEFLET+GV V T   SK FP FF+ S G  SP  +   E  AE+I       L+SG+L+AVPIPE     G  I  AI  ++  A+   + G  VTPF+L +++ +T G+SL  N+ LI NN +V +++A     ++KK  +           +  VVIGG  VD    +    +          T   S   + GGVGRN+A+    L     F+S +G D H + +      ++ S +   +   T   C +I+ E+ E+   +G++ I + I   ++  + E+L S    I LD N+P  T+ YV   AK + + V+ EPTD     KP          +  +   SPN+ E   M ++     PE DS  D++   S
Sbjct:   22 YDKLFKVHPSVREALAEHRPVVALESTIITHGMPYPHNLSTAKEVEAIVRAKGVTPATVGILDGTIHVGLSPEELDFM-----SRCKTALKVSRRDLPYVISKGLSGGTTVSGTMIAAHRAGIHVFVTGGIGGVHRDGENSLDVSADLTELGRTPIAVVSAGVKSILDIGRTLEFLETQGVCVATYGKSKSFPAFFSPSSGFTSPYSVHNAEEAAELIASTLSLGLQSGVLLAVPIPEEHAAAGQEIEGAIQAAVTEARLKGICGRDVTPFILQRVSELTEGQSLQGNIALIHNNAKVGSEIACALSERMKKCQMFLC--------ILQVVIGGVNVDCIAKVTKKLLFG-------QTNPGSVCQSFGGVGRNIADCLSRLGHKPLFISAVGTDTHSDAVLNYCKHMDTSAVVRLEGQRTATYCAVIT-ESGELSLGLGDMDIHQQIKEQHVCQYEEQLSSA-SLIVLDGNIPVSTINYVCSVAKKHSVPVWYEPTDADKACKPF-----LSESWKALSYTSPNLAELCTMNRTLHLPTPE-DSPEDVLSCAS 547
[Close]
BLAST of Pseudouridine-5'-phosphate glycosidase vs. Ensembl Cavefish
Match: zgc:136858 (zgc:136858 [Source:ZFIN;Acc:ZDB-GENE-060421-6632])

HSP 1 Score: 296.975 bits (759), Expect = 6.509e-88
Identity = 205/562 (36.48%), Postives = 304/562 (54.09%), Query Frame = 1 
Query:  145 FQELIETSEEISQSILDNKPTVALESTVITHGMPWPVNFETALELESIVKEEGAVPVTIGVLNGKLTAGFSKSELEELAQSVNSSCK-PIKISRHNI-YNSRNIKYGGTTASGTMIVAKELXXXXXXXXXXXXVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGVFVCTLD-SKVFPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEF-HKLKKTNIITTGKTSF------SSKLPIVVIGGCMVDNYMTIKDSSVVNLETIVPRATYDCSKVSTCGGVGRNLAEVFHLLNGTCHFLSVLGKDFHGEFIKTKSSQLNLSRIKYTDNYETGN-CTIISCENREILFHIGNISIFESITPDYLKGHLEELKSELKWICLDCNVPDKTMEYVIEYAKTNDIKVFIEPTDIMLVEKPVKVIKRFQSIFTPIDLISPNVFEFYQMIKSF----PE--SDSYSDLMEFDS 1779
            + +L +    + +++ +++P VALEST+ITHGMP+P N  TA E+E+IV+ +G  P T+G+L+G +  G S  EL+ +     S CK  +K+SR ++ Y       GGTT SGTMI A   GI++F TGGIGGVHR+ E+++D+S DL ELGRTPI VVS+G+KS LDI ++LEFLET+GV V T   SK FP FF+ S G  SP  +   E  AE+I       L+SG+L+AVPIPE     G  I  AI  ++  A+   + G  VTPF+L +++ +T G+SL  N+ LI NN +V +++A     ++KK      G +         SK   VVIGG  VD    +    +          T   S   + GGVGRN+A+    L     F+S +G D H + +      ++ S +   +   T   C +I+ E+ E+   +G++ I + I   ++  + E+L S    I LD N+P  T+ YV   AK + + V+ EPTD     KP          +  +   SPN+ E   M ++     PE   DS  D++   S
Sbjct:   24 YDKLFKVHPSVREALAEHRPVVALESTIITHGMPYPHNLSTAKEVEAIVRAKGVTPATVGILDGTIHVGLSPEELDFM-----SRCKTALKVSRRDLPYVISKGLSGGTTVSGTMIAAHRAGIHVFVTGGIGGVHRDGENSLDVSADLTELGRTPIAVVSAGVKSILDIGRTLEFLETQGVCVATYGKSKSFPAFFSPSSGFTSPYSVHNAEEAAELIASTLSLGLQSGVLLAVPIPEEHAAAGQEIEGAIQAAVTEARLKGICGRDVTPFILQRVSELTEGQSLQGNIALIHNNAKVGSEIACALSERMKKCQSGPRGSSEILEEKNQPSKPKPVVIGGVNVDCIAKVTKKLLFG-------QTNPGSVCQSFGGVGRNIADCLSRLGHKPLFISAVGTDTHSDAVLNYCKHMDTSAVVRLEGQRTATYCAVIT-ESGELSLGLGDMDIHQQIKEQHVCQYEEQLSSA-SLIVLDGNIPVSTINYVCSVAKKHSVPVWYEPTDADKACKPF-----LSESWKALSYTSPNLAELCTMNRTLHLPTPEVLPDSPEDVLSCAS 566
[Close]
BLAST of Pseudouridine-5'-phosphate glycosidase vs. Ensembl Sea Lamprey
Match: zgc:136858 (zgc:136858 [Source:ZFIN;Acc:ZDB-GENE-060421-6632])

HSP 1 Score: 244.588 bits (623), Expect = 2.158e-71
Identity = 166/436 (38.07%), Postives = 245/436 (56.19%), Query Frame = 1 
Query:  457 GGTTASGTMIVAKELXXXXXXXXXXXXVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGVFVCTL-DSKVFPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEFHKLKKTNIITTGKTSFSSKLPI------VVIGGCMVDNYMTIKDSSV-----VNLETIVPRATYDCSKVSTCGGVGR-NLAEVFHLLNGTCHFLSVLGKDFHGEFIKTKSSQLNLSRIKYTDNYETGNCTIISCENREILFHIGNISIFESITPDYLKGHLEELKSELKWICLDCNVPDKTMEYVIEYAKTNDIKVFIEPTDIMLVEKPVKVIKRFQSIFTPIDLISPNVFEFYQM 1725
            GGTT SGTM+ A + GI++F TGGIGGVHR+V+S++DIS DL ELGRTPI VVS+GIKS LDI ++LE+LET+GV V T   S+ FP FFT   G  SP +++T E  AE+I    +  L SG+L+AVP+PE    +G +I  AI ++L  A   ++ G  VTPF+L ++N +T G+SL  N+ LIKNN  V A +A +    +K +   +G+++  + LP       VVIGGC VD    IK S++      N  T+  R ++        GGVG    A+    L     F+S +G+D     + + SS ++ S I       T     +  +  E+   IG++ +   IT DY++G  +++ S    +C+D N+P  T++YV    K   + V+ EPTDI   +KP          +  + + SPN+ E   M
Sbjct:    5 GGTTVSGTMVAAHKAGIHVFVTGGIGGVHRDVQSSLDISADLTELGRTPIAVVSAGIKSILDIGRTLEYLETQGVCVTTFGSSRDFPAFFTPRSGFLSPYNVETEEEAAELIASGLRLGLGSGVLIAVPVPERHAVEGQLIEAAIQQALREATEQQIVGKEVTPFILRRVNELTQGQSLEANIALIKNNADVGAGIACKLS--EKQHGAPSGRSTLRA-LPKEQDKNNVVIGGCNVDFVAKIKSSNIQFGGQTNYGTV--RQSF--------GGVGAVCCADGLARLGTNPLFISAVGEDDLARMLISNSSHMDTSGIARVAGASTATYCAVLDDAGELTVGIGDMDVHRRITHDYVQGFEQQIGSAC-IVCVDGNLPVSTIDYVCGVGKERGVPVWFEPTDIDKAKKPF-----LSGSWRGLSVTSPNLAELLAM 421
[Close]
BLAST of Pseudouridine-5'-phosphate glycosidase vs. Ensembl Nematostella
Match: EDO34183 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQ37])

HSP 1 Score: 260.766 bits (665), Expect = 1.393e-76
Identity = 182/489 (37.22%), Postives = 264/489 (53.99%), Query Frame = 1 
Query:  268 ALELESIVKEEGAVPVTIGVLNGKLTAGFSKSELEELAQSVNSSCKPIKISRHNI-YNSRNIKYGGTTASGTMIVAKELXXXXXXXXXXXXVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGVFVCTLDSKV-FPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEFHKLKKTNIITTGKTSFSSKLPIVVIGGCMVDNYMTIKDSSVVNLETIVPRATYDCSKVS-TCGGVGRNLAEVFHLLNGTCHFLSVLGKDFHGEFIKTKSSQLNLSRIKYTDNYETGNCTIISCENREILFHIGNISIFESITPDYLKGHLEELKSELKWICLDCNVPDKTMEYVIEYAKTNDIKVFIEPTDIMLVEKPVKVIKRFQSIFTPIDLISPNVFEFYQM 1725
            A ++  +V++ GAVP TI +L+G++  G +  +L++LAQ+  ++   +K SR ++ Y       GGTT SGTMI A + GI +F TGGIGGVHR  + + D+S DL ELGRTPI VV +G KS LDI  +LE+LET+GV V T    V FP FFT   G KS   +      A +I     F + SGM++AVPIP      G  I +AI ++LE A+SA +TG   TPFVL ++N +T GKSL  N+ L+K+N ++ +++A EF KL++ NI  +     S     VVIGG  VD   T K+  V          T +  KV  + GGVGRN+AE    L     F+S +G D  G  +     +  L  I+     ETG  + I  E+      +G++ I + I+ + + G+ E +K+    + +D NV    ++YV+       I V+ EPT IM    P +        +  I   SPN+ E   M
Sbjct:    2 ARDVCGVVRDNGAVPATIAILDGQIHVGLTDDKLKKLAQAGQNA---VKTSRRDLPYVLSKGLMGGTTVSGTMIAAHKAGIPVFVTGGIGGVHRGAQESFDVSADLTELGRTPIAVVCAGAKSILDIQLTLEYLETQGVTVATFGETVNFPAFFTSDSGYKSSCSVLNASEAAAMINCNLTFGVGSGMVIAVPIPSGYAASGAEIEKAIQQALEEARSANITGKESTPFVLKRVNELTGGKSLEANIALVKHNAKIGSQIAVEFSKLRQPNIAKSAPIRDS-----VVIGGANVDFIATAKEIVV----------TSNSGKVRMSFGGVGRNIAEGLSRLGMKPVFVSAVGGDSLGALLLRHCEEFRLRGIQTFQQCETGTYSAIISEDGSFHAAVGDMEIHDKISQEMIAGYEESIKNA-PIVVVDANVSRSGLDYVLNLCSEQSIPVWFEPTCIMKAGIPFET-----DCWKHITYASPNLKELRTM 466
[Close]
BLAST of Pseudouridine-5'-phosphate glycosidase vs. Ensembl Nematostella
Match: EDO27168 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TA04])

HSP 1 Score: 51.9878 bits (123), Expect = 2.495e-8
Identity = 41/94 (43.62%), Postives = 62/94 (65.96%), Query Frame = 1 
Query:  268 ALELESIVKEEGAVPVTIGVLNGKLTAGFSKSELEELAQSVNSSCKPIKISRHNI-YNSRNIKYGGTTASGTMIVAKELXXXXXXXXXXXXVHR 546
            A ++  +V++ GAVP TI +L+G++  G +  +L++LAQ+  ++   +K SR ++ Y       GGTT SGTMI A + GI +F TGGIGGVHR
Sbjct:    2 ARDVCGVVRDNGAVPATIAILDGQIHVGLTDDKLKKLAQAGQNA---VKTSRRDLPYVLSKGLMGGTTVSGTMIAAHKAGIPVFVTGGIGGVHR 92
[Close]
BLAST of Pseudouridine-5'-phosphate glycosidase vs. Ensembl Medaka
Match: zgc:136858 (pseudouridine-metabolizing bifunctional protein C1861.05 [Source:NCBI gene;Acc:101173144])

HSP 1 Score: 322.398 bits (825), Expect = 3.103e-98
Identity = 208/528 (39.39%), Postives = 298/528 (56.44%), Query Frame = 1 
Query:  175 ISQSILDNKPTVALESTVITHGMPWPVNFETALELESIVKEEGAVPVTIGVLNGKLTAGFSKSELEELAQSVNSSCKPIKISRHNI-YNSRNIKYGGTTASGTMIVAKELXXXXXXXXXXXXVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGVFVCTLD-SKVFPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEFHKLKK---TNIITTGKTSFS--SKLPIVVIGGCMVDNYMTIKDSSVVNLETIVPRATYDCSKVSTCGGVGRNLAEVFHLLNGTCHFLSVLGKDFHGEFIKTKSSQLNLSRIKYTDNYETGNCTIISCENREILFHIGNISIFESITPDYLKGHLEELKSELKWICLDCNVPDKTMEYVIEYAKTNDIKVFIEPTDIMLVEKPVKVIKRFQSIFTPIDLISPNVFEFYQMIKSF 1737
            +SQ++ +NKP VALEST+ITHGMP+P N  TA E+E+IV+ EGA P T+GV+ GK+  G S S+LE LAQ  N+    +K+SR ++ Y       GGTT S TMI A  +GI +F TGGIGGVHR+  +++DIS DL ELGRTPI VVS+GIKS LDI ++LEFLET+GV V T   +K FP FF+   G  S   +      A +I       L++G+L+AVPIPE     G  I +AI  ++  A S  V G  VTPF+L ++N +T GKSL  N+ LI+NN +V +++A    K      T I    + + S  +K  IVVIGG  VD ++    +  +N     P      S   + GGVGRN+A+    L     F+S  G D + + +      +N S +   + + T    ++  E+ E+ F +G++ I + IT +Y+    E+  S    +CLD N+P  T+ Y+   AK ++I V+ EPTD+    KP          +  +   SPN+ E   M K+ 
Sbjct:   36 VSQALAENKPVVALESTIITHGMPYPHNLSTANEVEAIVQSEGATPATVGVIQGKIHVGLSPSDLEHLAQCRNA----LKVSRRDLPYVISKGLSGGTTVSATMIAAHRVGIPVFVTGGIGGVHRDGHNSLDISADLTELGRTPIAVVSAGIKSILDIGRTLEFLETQGVCVATYGMTKSFPAFFSPLSGFSSAYHVCNPSEAASLIASSLSLGLQNGVLIAVPIPEEHAAAGQHIEEAIQAAVREASSKCVIGKDVTPFILQKVNELTQGKSLQANMALIRNNAKVGSQIACALSKQTAKTSTKIQNQHRRNLSTNTKSDIVVIGGINVD-FIAKGKTKKINFGQTNPG-----SVCQSFGGVGRNIADSLSRLGQAPLFISATGCDANSDAVFNYCKHMNTSGVARLEGHNTATYCVVISESGELSFGLGDMDIHQHITENYVS-QFEKQVSSAALVCLDGNIPVSTINYICSLAKKHNINVWYEPTDVERASKPF-----LTDAWKSLSYSSPNMAELCNMNKTL 547
[Close]
BLAST of Pseudouridine-5'-phosphate glycosidase vs. Ensembl Medaka
Match: zgc:136858 (pseudouridine-metabolizing bifunctional protein C1861.05 [Source:NCBI gene;Acc:101173144])

HSP 1 Score: 295.819 bits (756), Expect = 1.156e-88
Identity = 199/523 (38.05%), Postives = 285/523 (54.49%), Query Frame = 1 
Query:  175 ISQSILDNKPTVALESTVITHGMPWPVNFETALELESIVKEEGAVPVTIGVLNGKLTAGFSKSELEELAQSVNSSCKPIKISRHNI-YNSRNIKYGGTTASGTMIVAKELXXXXXXXXXXXXVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGVFVCTLD-SKVFPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEFHKLKKTNIITTGKTSFSSKLPIVVIGGCMVDNYMTIKDSSVVNLETIVPRATYDCSKVSTCGGVGRNLAEVFHLLNGTCHFLSVLGKDFHGEFIKTKSSQLNLSRIKYTDNYETGNCTIISCENREILFHIGNISIFESITPDYLKGHLEELKSELKWICLDCNVPDKTMEYVIEYAKTNDIKVFIEPTDIMLVEKPVKVIKRFQSIFTPIDLISPNVFEFYQMIKSF 1737
            +SQ++ +NKP VALEST+ITHGMP+P N  TA E+E+IV+ EGA P T+GV+ GK+  G S S+LE LAQ  N+    +K+SR ++ Y       GGTT S TMI A  +GI +F TGGIGGVHR+  +++DIS DL ELGRTPI VVS+GIKS LDI ++LEFLET+GV V T   +K FP FF+   G  S   +      A +I       L++G+L+AVPIPE     G  I +AI  ++  A S  V G  VTPF+L ++N +T GKSL    + I +N ++            K  I+             VVIGG  VD ++    +  +N     P      S   + GGVGRN+A+    L     F+S  G D + + +      +N S +   + + T    ++  E+ E+ F +G++ I + IT +Y+    E+  S    +CLD N+P  T+ Y+   AK ++I V+ EPTD+    KP          +  +   SPN+ E   M K+ 
Sbjct:   34 VSQALAENKPVVALESTIITHGMPYPHNLSTANEVEAIVQSEGATPATVGVIQGKIHVGLSPSDLEHLAQCRNA----LKVSRRDLPYVISKGLSGGTTVSATMIAAHRVGIPVFVTGGIGGVHRDGHNSLDISADLTELGRTPIAVVSAGIKSILDIGRTLEFLETQGVCVATYGMTKSFPAFFSPLSGFSSAYHVCNPSEAASLIASSLSLGLQNGVLIAVPIPEEHAAAGQHIEEAIQAAVREASSKCVIGKDVTPFILQKVNELTQGKSL----QAILSNSKITQ---------TKGGILLQ-----------VVIGGINVD-FIAKGKTKKINFGQTNPG-----SVCQSFGGVGRNIADSLSRLGQAPLFISATGCDANSDAVFNYCKHMNTSGVARLEGHNTATYCVVISESGELSFGLGDMDIHQHITENYVS-QFEKQVSSAALVCLDGNIPVSTINYICSLAKKHNINVWYEPTDVERASKPF-----LTDAWKSLSYSSPNMAELCNMNKTL 516
[Close]
BLAST of Pseudouridine-5'-phosphate glycosidase vs. Planmine SMEST
Match: SMESG000061340.1 (SMESG000061340.1)

HSP 1 Score: 1330.85 bits (3443), Expect = 0.000e+0
Identity = 703/705 (99.72%), Postives = 703/705 (99.72%), Query Frame = 1 
Query:   82 MLNKLIKFTSKPLLFKRCFNGFQELIETSEEISQSILDNKPTVALESTVITHGMPWPVNFETALELESIVKEEGAVPVTIGVLNGKLTAGFSKSELEELAQSVNSSCKPIKISRHNIYNSRNIKYGGTTASGTMIVAKELXXXXXXXXXXXXVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGVFVCTLDSKVFPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEFHKLKKTNIITTGKTSFSSKLPIVVIGGCMVDNYMTIKDSSVVNLETIVPRATYDCSKVSTCGGVGRNLAEVFHLLNGTCHFLSVLGKDFHGEFIKTKSSQLNLSRIKYTDNYETGNCTIISCENREILFHIGNISIFESITPDYLKGHLEELKSELKWICLDCNVPDKTMEYVIEYAKTNDIKVFIEPTDIMLVEKPVKVIKRFQSIFTPIDLISPNVFEFYQMIKSFPESDSYSDLMEFDSKSFEFNQTKIFEXXXXXXXXXXXXXXIPKWIIKLGPAGVVFASKTEMFFIEQSDSFVNKQDIINTSXXXXXXXXXXXXXXXKTDLSWKHKIAGSMRLVKESLTNNSTVPVGSDFNNYTESEIRKWTEEMFDRKLLVYQE 2196
            MLNKLIKFTSKPLLFKRCFNGFQELIETSEEISQSILDNKPTVALESTVITHGMPWPVNFETALELESIVKEEGAVPVTIGVLNGKLTAGFSK ELEELAQSVNSSCKPIKISRHNIYNSRNIKYGGTTASGTMIVAKELGINIFGTGGIGGVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGVFVCTLDSKVFPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEFHKLKKTNIITTGKTSF SKLPIVVIGGCMVDNYMTIKDSSVVNLETIVPRATYDCSKVSTCGGVGRNLAEVFHLLNGTCHFLSVLGKDFHGEFIKTKSSQLNLSRIKYTDNYETGNCTIISCENREILFHIGNISIFESITPDYLKGHLEELKSELKWICLDCNVPDKTMEYVIEYAKTNDIKVFIEPTDIMLVEKPVKVIKRFQSIFTPIDLISPNVFEFYQMIKSFPESDSYSDLMEFDSKSFEFNQTKIFENLKLLMKNSNLLDLIPKWIIKLGPAGVVFASKTEMFFIEQSDSFVNKQDIINTSGAGDCLAGCALLLHLKTDLSWKHKIAGSMRLVKESLTNNSTVPVGSDFNNYTESEIRKWTEEMFDRKLLVYQE
Sbjct:    1 MLNKLIKFTSKPLLFKRCFNGFQELIETSEEISQSILDNKPTVALESTVITHGMPWPVNFETALELESIVKEEGAVPVTIGVLNGKLTAGFSKLELEELAQSVNSSCKPIKISRHNIYNSRNIKYGGTTASGTMIVAKELGINIFGTGGIGGVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGVFVCTLDSKVFPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLVAVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNGKSLMLNVELIKNNVRVAAKLAKEFHKLKKTNIITTGKTSFGSKLPIVVIGGCMVDNYMTIKDSSVVNLETIVPRATYDCSKVSTCGGVGRNLAEVFHLLNGTCHFLSVLGKDFHGEFIKTKSSQLNLSRIKYTDNYETGNCTIISCENREILFHIGNISIFESITPDYLKGHLEELKSELKWICLDCNVPDKTMEYVIEYAKTNDIKVFIEPTDIMLVEKPVKVIKRFQSIFTPIDLISPNVFEFYQMIKSFPESDSYSDLMEFDSKSFEFNQTKIFENLKLLMKNSNLLDLIPKWIIKLGPAGVVFASKTEMFFIEQSDSFVNKQDIINTSGAGDCLAGCALLLHLKTDLSWKHKIAGSMRLVKESLTNNSTVPVGSDFNNYTESEIRKWTEEMFDRKLLVYQE 705
The following BLAST results are available for this feature:
BLAST of Pseudouridine-5'-phosphate glycosidase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pseudouridine-5'-phosphate glycosidase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
T24C12.39.367e-8234.43pep chromosome:WBcel235:X:2196525:2203961:-1 gene:... [more]
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BLAST of Pseudouridine-5'-phosphate glycosidase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CG27941.902e-10137.94gene:FBgn0031265 transcript:FBtr0078067[more]
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BLAST of Pseudouridine-5'-phosphate glycosidase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zgc:1368588.668e-9437.93zgc:136858 [Source:ZFIN;Acc:ZDB-GENE-060421-6632][more]
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BLAST of Pseudouridine-5'-phosphate glycosidase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSXETT00000005835.11.011e-9037.59pseudouridine-metabolizing bifunctional protein C1... [more]
ENSXETT00000005726.11.196e-5833.93pseudouridine-metabolizing bifunctional protein C1... [more]
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BLAST of Pseudouridine-5'-phosphate glycosidase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pseudouridine-5'-phosphate glycosidase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9USY1|YOW5_SCHPO9.592e-6931.91Pseudouridine-metabolizing bifunctional protein C1... [more]
sp|Q1M4T3|PSUG2_RHIL33.075e-6843.46Pseudouridine-5'-phosphate glycosidase 2 OS=Rhizob... [more]
sp|A7GCK7|PSUG_CLOBL1.069e-6644.66Pseudouridine-5'-phosphate glycosidase OS=Clostrid... [more]
sp|C0ZIY1|PSUG_BREBN1.643e-6644.48Pseudouridine-5'-phosphate glycosidase OS=Brevibac... [more]
sp|A6TL60|PSUG_ALKMQ2.558e-6241.75Pseudouridine-5'-phosphate glycosidase OS=Alkaliph... [more]
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BLAST of Pseudouridine-5'-phosphate glycosidase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0L0BXN52.012e-10740.40PfkB domain-containing protein OS=Lucilia cuprina ... [more]
A0A0M4E8768.353e-10439.19CG2794 OS=Drosophila busckii OX=30019 GN=Dbus_chr2... [more]
A0A210PPV37.066e-10338.81PfkB domain-containing protein OS=Mizuhopecten yes... [more]
W8C0I54.756e-10138.56Pseudouridine-metabolizing bifunctional protein C1... [more]
A0A4D9E7735.112e-10039.96Protein brambleberry-like OS=Platysternon megaceph... [more]
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BLAST of Pseudouridine-5'-phosphate glycosidase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
zgc:1368588.253e-8936.46zgc:136858 [Source:ZFIN;Acc:ZDB-GENE-060421-6632][more]
zgc:1368586.509e-8836.48zgc:136858 [Source:ZFIN;Acc:ZDB-GENE-060421-6632][more]
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BLAST of Pseudouridine-5'-phosphate glycosidase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zgc:1368582.158e-7138.07zgc:136858 [Source:ZFIN;Acc:ZDB-GENE-060421-6632][more]
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BLAST of Pseudouridine-5'-phosphate glycosidase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pseudouridine-5'-phosphate glycosidase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO341831.393e-7637.22Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO271682.495e-843.62Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Pseudouridine-5'-phosphate glycosidase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
zgc:1368583.103e-9839.39pseudouridine-metabolizing bifunctional protein C1... [more]
zgc:1368581.156e-8838.05pseudouridine-metabolizing bifunctional protein C1... [more]
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BLAST of Pseudouridine-5'-phosphate glycosidase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000061340.10.000e+099.72SMESG000061340.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031474 ID=SMED30031474|Name=Pseudouridine-5'-phosphate glycosidase|organism=Schmidtea mediterranea sexual|type=transcript|length=2511bp
AGCTCACTATACCAAAGATAAAGTTGTTATTAAAATATAAACGGATAATG
GTCGAACTTTTATGAATATTTTTATGTGCAAATGTTAAACAAATTGATAA
AATTTACATCGAAACCTTTGCTATTTAAAAGATGTTTCAACGGTTTTCAA
GAATTAATCGAGACATCCGAAGAGATATCTCAGTCAATTCTAGATAATAA
GCCAACTGTTGCATTGGAAAGTACAGTGATTACTCACGGAATGCCATGGC
CGGTCAACTTTGAAACAGCTTTAGAGTTAGAAAGTATTGTTAAAGAAGAA
GGGGCAGTTCCGGTGACAATTGGAGTATTGAATGGAAAATTAACTGCTGG
ATTTTCAAAATCGGAATTGGAAGAATTGGCTCAGTCAGTTAATTCCAGTT
GTAAACCAATTAAAATATCTCGTCACAATATTTATAATAGTAGAAATATA
AAGTATGGGGGGACAACAGCATCAGGAACGATGATTGTTGCAAAAGAATT
GGGGATTAATATTTTTGGAACAGGTGGCATTGGCGGTGTTCACAGGGAAG
TTGAAAGTACCATGGATATTAGCACGGATCTTCGAGAATTGGGAAGAACT
CCAATTTGTGTCGTTTCGTCTGGTATCAAGTCATTTTTAGACATTCCAAA
ATCTCTAGAGTTTTTAGAAACTGAGGGTGTCTTTGTGTGCACTTTAGATT
CCAAAGTCTTTCCGAATTTCTTCACTCGTTCTTGTGGAATCAAATCCCCA
AACGATATAAAAACAATTGAAAATGTGGCCGAAATTATTTTTGACATGAC
GAAATTTCAGTTAAAATCTGGCATGCTTGTGGCAGTTCCGATACCGGAAT
GTTTGGAAAAGGATGGCAACATTATTCACCAAGCAATCATTAAGAGTTTA
GAAAATGCAAAATCTGCCAAGGTCACTGGGAGTTATGTAACTCCATTTGT
TTTAAATCAAATAAATATTATTACGAATGGGAAATCACTAATGTTGAATG
TTGAATTAATTAAAAACAATGTTCGGGTAGCAGCAAAATTAGCAAAAGAA
TTTCACAAATTAAAGAAAACGAACATAATCACAACTGGAAAAACAAGCTT
TAGCAGTAAGTTACCAATCGTCGTGATTGGAGGTTGTATGGTGGATAACT
ACATGACAATTAAAGATTCGTCAGTAGTCAACCTCGAAACAATAGTTCCT
CGTGCCACTTACGATTGCTCTAAAGTTTCGACTTGTGGTGGAGTCGGGAG
AAATTTAGCGGAAGTTTTTCATTTATTAAATGGTACTTGTCATTTTCTTT
CAGTTTTAGGAAAGGATTTTCATGGTGAATTCATTAAAACCAAATCGAGT
CAACTCAATCTCTCAAGAATTAAATACACAGACAATTATGAAACCGGAAA
TTGTACGATAATTTCCTGTGAGAATCGTGAAATTTTATTCCACATAGGAA
ATATTTCAATTTTTGAAAGCATTACTCCAGATTATTTGAAGGGTCATTTA
GAAGAACTTAAATCCGAGTTAAAATGGATTTGTTTAGACTGTAATGTTCC
TGACAAAACGATGGAATATGTGATTGAATACGCTAAAACAAACGACATAA
AGGTTTTCATCGAACCCACCGATATAATGCTTGTGGAAAAACCGGTTAAA
GTCATTAAAAGATTTCAATCAATTTTCACCCCCATAGACCTAATTTCACC
GAATGTTTTTGAATTTTATCAAATGATCAAATCCTTTCCGGAATCCGACA
GTTATTCGGATCTTATGGAATTCGATTCCAAATCATTCGAATTCAATCAA
ACAAAAATCTTTGAAAATTTGAAATTATTAATGAAAAATTCGAATTTACT
AGATTTGATTCCAAAGTGGATAATCAAACTAGGACCGGCCGGAGTAGTTT
TTGCATCGAAAACCGAAATGTTTTTCATAGAACAATCGGACAGTTTCGTT
AATAAACAAGATATTATCAACACATCTGGAGCGGGAGATTGTTTGGCCGG
TTGTGCTTTATTGTTACATCTGAAAACAGATCTATCTTGGAAACACAAAA
TAGCCGGCAGCATGAGACTAGTGAAAGAATCCTTAACCAATAACAGCACC
GTCCCAGTCGGAAGCGATTTCAATAACTACACTGAATCGGAAATCCGGAA
ATGGACTGAAGAAATGTTTGACAGAAAACTGTTAGTTTATCAAGAATAAA
ATAAATTTTTAATTAACTGTTTTCGCTGTCGAAAAGTCGAATTTATTTTT
TTTCACTTTCTACATGAGAGAAAACTTTAAATTACATATCAGTTCGTCGA
TGTCAATGAAGCATCTGTGAATTCATAATGCTAATTTCAAGCCTTATTAG
TTTCAGCAATTATTTATCGAAAGGTGGAGTGGTTTTCCATATTTACTGAA
TCGAAATTTCTAGATAAAAAAATTTTGTTTAGTTTACGTTTTTATACTTT
TTCTTTTCTTAGAAATTCTGAAAATAATAATTTGAATTAGCTTATAACAA
TTGAATATCCC
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protein sequence of SMED30031474-orf-1

>SMED30031474-orf-1 ID=SMED30031474-orf-1|Name=SMED30031474-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=706bp
MLNKLIKFTSKPLLFKRCFNGFQELIETSEEISQSILDNKPTVALESTVI
THGMPWPVNFETALELESIVKEEGAVPVTIGVLNGKLTAGFSKSELEELA
QSVNSSCKPIKISRHNIYNSRNIKYGGTTASGTMIVAKELGINIFGTGGI
GGVHREVESTMDISTDLRELGRTPICVVSSGIKSFLDIPKSLEFLETEGV
FVCTLDSKVFPNFFTRSCGIKSPNDIKTIENVAEIIFDMTKFQLKSGMLV
AVPIPECLEKDGNIIHQAIIKSLENAKSAKVTGSYVTPFVLNQINIITNG
KSLMLNVELIKNNVRVAAKLAKEFHKLKKTNIITTGKTSFSSKLPIVVIG
GCMVDNYMTIKDSSVVNLETIVPRATYDCSKVSTCGGVGRNLAEVFHLLN
GTCHFLSVLGKDFHGEFIKTKSSQLNLSRIKYTDNYETGNCTIISCENRE
ILFHIGNISIFESITPDYLKGHLEELKSELKWICLDCNVPDKTMEYVIEY
AKTNDIKVFIEPTDIMLVEKPVKVIKRFQSIFTPIDLISPNVFEFYQMIK
SFPESDSYSDLMEFDSKSFEFNQTKIFENLKLLMKNSNLLDLIPKWIIKL
GPAGVVFASKTEMFFIEQSDSFVNKQDIINTSGAGDCLAGCALLLHLKTD
LSWKHKIAGSMRLVKESLTNNSTVPVGSDFNNYTESEIRKWTEEMFDRKL
LVYQE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000421tail
PLANA:0002075pigment cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR022830Indigdn_synthA-like
IPR007342PsuG
IPR029056Ribokinase-like
IPR011611PfkB_dom
Vocabulary: molecular function
TermDefinition
GO:0016798hydrolase activity, acting on glycosyl bonds
GO:0016773phosphotransferase activity, alcohol group as acceptor
Vocabulary: biological process
TermDefinition
GO:0008152metabolic process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029056Ribokinase-likeGENE3DG3DSA:3.40.1190.20coord: 342..682
e-value: 1.3E-37
score: 131.6
IPR029056Ribokinase-likeSUPERFAMILYSSF53613Ribokinase-likecoord: 344..647
IPR011611Carbohydrate kinase PfkBPFAMPF00294PfkBcoord: 382..643
e-value: 2.4E-17
score: 63.2
IPR007342Pseudouridine-5'-phosphate glycosidasePFAMPF04227Indigoidine_Acoord: 31..323
e-value: 1.1E-103
score: 346.6
IPR007342Pseudouridine-5'-phosphate glycosidasePANTHERPTHR42909FAMILY NOT NAMEDcoord: 20..400
IPR022830Indigoidine synthase A-likeGENE3DG3DSA:3.40.1790.10coord: 22..329
e-value: 8.6E-111
score: 371.9
IPR022830Indigoidine synthase A-likeSUPERFAMILYSSF110581Indigoidine synthase A-likecoord: 24..324