SMED30031472
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30031472 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Alignments
SMED30031472 aligns in the following genomic locations:
Homology
BLAST of SMED30031472 vs. TrEMBL
Match: A0A564YUZ6 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS9905 PE=4 SV=1) HSP 1 Score: 67.3958 bits (163), Expect = 3.071e-8 Identity = 75/310 (24.19%), Postives = 130/310 (41.94%), Query Frame = 3 Query: 174 VTMLLVISCIVSLMMVIPITIQYIGS---ILDYRLRLQYQIEDTEESD-----------NPIEKYEYYLYHYRIMFNLSIFLTIIGSSLPTSIYMTQRNPDEMSTAVSAYEEFINIWGVCHLMTYLDICGRIFFSLIITVPSLIIFIYVFFKNYEP------KVIFRSIYISSWVSLLFALISPITFVTNEYEKFTIENRSLTYCKQGVELTVYNRCRLFELLCVHLIPLAIQIPCIVMIKKS----LTFANNSLLYEPIIFIVTNIPIAIIDT---IEQMDEKWIH--SSEFSNFYILFLTIYHLGYTC 1016 T+LL +V ++++IP +Q + I R +L Q + +S + L+ YRI+ ++ + +I S+LP + P E + +WG CH+ Y+D R F + +I +P ++ F F P K IFR I IS+ LF+ + I V YEK I N S+ YC G +T F+ + P I I+++ + L + + YEP+IF+ P+ ++ I K H + +F+N ++F YH+ ++ Sbjct: 52 ATLLLSGFLVVGILLIIPAIMQTSAAYLWIGQVRKQLGRQPRSSVDSGRYSTVPSIFRKKNVTDMTRTLFVYRILACVASLMVLICSTLPQLVLTVANRPKEGN--------IDQLWGWCHIFVYMDHTTRSFATYLIVIPYVLFFWDFLFSEVVPRRHLVMKRIFRGILISALPIALFSSMVAIPLVIYRYEK--IGNDSIIYC--GATITGTTNFIAFDFAVTRVAPTVI----IIVVTSAFLIWLPRHHYGVFYEPLIFLCLMAPVVFLEGTVYIACYMGKVTHLINEQFANAVLIFYAFYHMSFSS 345
BLAST of SMED30031472 vs. TrEMBL
Match: A0A0R3SBQ4 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS1932 PE=4 SV=1) HSP 1 Score: 65.0846 bits (157), Expect = 1.769e-7 Identity = 63/242 (26.03%), Postives = 106/242 (43.80%), Query Frame = 3 Query: 336 LYHYRIMFNLSIFLTIIGSSLPTSIYMTQRNPDEMSTAVSAYEEFINIWGVCHLMTYLDICGRIFFSLIITVPSLIIFIYVFFKNYEP------KVIFRSIYISSWVSLLFALISPITFVTNEYEKFTIENRSLTYCKQGVELTVYNRCRLFELLCVHLIPLAIQIPCIVMIKKS----LTFANNSLLYEPIIFIVTNIPIAIIDT---IEQMDEKWIH--SSEFSNFYILFLTIYHLGYTC 1016 L+ YRI+ ++ + +I S+LP + P E + +WG CH+ Y+D R F + +I +P ++ F F P K IFR I IS+ LF+ + I V YEK I N S+ YC G +T F+ + P I I+++ + L + + YEP+IF+ P+ ++ I K H + +F+N ++F YH+ ++ Sbjct: 49 LFVYRILACVASLMVLICSTLPQLVLTVANRPKEGN--------IDQLWGWCHIFVYMDHTTRSFATYLIVIPYVLFFWDFLFSEVVPRRHLVMKRIFRGILISALPIALFSSMVAIPLVIYRYEK--IGNDSIIYC--GATITGTTNFIAFDFAVTRVAPTVI----IIVVTSAFLIWLPRHHYGVFYEPLIFLCLMAPVVFLEGTVYIACYMGKVTHLINEQFANAVLIFYAFYHMSFSS 274
BLAST of SMED30031472 vs. TrEMBL
Match: A0A4E0RFS5 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_002536 PE=4 SV=1) HSP 1 Score: 63.5438 bits (153), Expect = 4.116e-7 Identity = 76/323 (23.53%), Postives = 141/323 (43.65%), Query Frame = 3 Query: 171 FVTMLLVISCIVSLMMVIPITIQYIGSIL-------DYRLRLQYQIEDT---EES-----------DNPIEKYEYYLYHYRIMFNLSIFLTIIGSSLPTSIYMTQR--NPDEMSTAVSAYEEFINIWGVCHLMTYLDICGRIFFSLIITVPSLIIFIYVFFKNYEPKV------IFRSIYISSWVSLLFALISPITFVTNEYEKFTIENRSLTYCK---QGVELTVYNRCRLFELLCVHLIPLAIQIPCIVMIKKS----LTFANNSLLYEPIIFIVTNIPIAIIDTIEQ------MDEKWIHSSEFSNFYILFLTIYHLGYT 1013 F T LL +V ++++IP +Q + + + R++ Q D +ES + + K + L+ YR++ + L +I S+LP I R +P+ + +WG CH++TY D R F + +I VP ++F F P+ +F I +S LF+ I P+ V + ++ E +T C G+ +Y ++ L +IP+A CI++ L + + YEP IF + ++PI + +T+ + +K I + FSNF ++ +YH+ ++ Sbjct: 42 FATCLLSGFFVVGILLIIPALMQTAAAYVWAAQVRKEASSRMKAQESDQRGLQESTHSAKHVYINDGHRLSKQDRTLFLYRLLACCASLLVLIFSTLPQLIIGVARPLSPNTID----------KLWGWCHVLTYTDASTRCFATYLIVVPFGLLFWNFIFGEILPRPQLLTDNVFAVILRASVPIALFSFIIPVPMVAKRFVQYYDEG--MTKCNGSPDGLVFMIY-----YDYLVTRVIPVA----CILVFSIGFVLWLPRKDYGVFYEPGIFTIFSVPIILCETVIHVYHRIGLVDKLIEPN-FSNFMLIAYALYHMSFS 342
BLAST of SMED30031472 vs. TrEMBL
Match: A0A068WDM5 (Uncharacterized protein OS=Echinococcus granulosus OX=6210 GN=EgrG_000812600 PE=4 SV=1) HSP 1 Score: 63.929 bits (154), Expect = 4.346e-7 Identity = 76/362 (20.99%), Postives = 150/362 (41.44%), Query Frame = 3 Query: 174 VTMLLVISCIVSLMMVIPITIQYIGS---ILDYRLRLQYQ----IEDTEESDNP-------IEKYEYYLYHYRIMFNLSIFLTIIGSSLPTSIYMTQRNPDEMSTAVSAYEEFINIWGVCHLMTYLDICGRIFFSLIITVPSLIIFIYVFFKNYEPKV------IFRSIYISSWVSLLFALISPITFVTNEYEKFTIENRSLTYCKQGVELTVYNRCRLFELLCVHLIPLAIQIPCIVMIKKSLTFANNSLLYEPIIFIVTNIPIAIIDTIEQMD-----EKWIHSSEFSNFYILFLTIYHLGYTCMILVWPFQMI--WMNKDENLQIKPEINDVYKILTNRKTIRKTV----RRQFTAIKR 1166 T++L +V ++++IP +Q + I R +L +Q + S P + L+ YRI+ ++ L ++ S+LP + P E +WG CH Y+D R F + +I VP ++ F F P+ IFR I IS+ LF+ + + V YE+ + N + C + T +F+ ++P+AI + L + + YEP+IF+ P+A ++ + +++ + F+N ++F YH+ ++ ++ + + + ++ + + I + K+ R R V +RQF+ + R Sbjct: 65 ATLILSGFLVVGILLIIPAILQTAAAYLWIGQCRKQLGHQPLINSRNNGYSTVPTGFPQKNVTDMTRTLFIYRILACVASLLVLLCSTLPQLVLAVANPPKE--------GNIDQLWGWCHAFVYMDHATRSFATYLIVVPYVLFFWDFLFSEVVPRRRLVMQHIFRGILISALPIALFSSLIAVPLVLFRYER--LWNGTHDVCLSSISGTKI--FLVFDFAVTRVVPMAIIVGVTSAFLIWLPRHHYGVFYEPLIFLFLMAPLAFVEATIYIACYMGVVRYLVNENFANALLIFYAFYHMSFSSTFVLATLRSVVTEIRRERRARKQFSIGEEPKVDECRSARRSNVMLGLQRQFSVVFR 414
BLAST of SMED30031472 vs. TrEMBL
Match: A0A504YWG9 (Uncharacterized protein OS=Fasciola gigantica OX=46835 GN=FGIG_02586 PE=4 SV=1) HSP 1 Score: 63.5438 bits (153), Expect = 4.826e-7 Identity = 75/323 (23.22%), Postives = 141/323 (43.65%), Query Frame = 3 Query: 171 FVTMLLVISCIVSLMMVIPITIQYIGS-ILDYRLRLQYQIE-DTEESD-------------------NPIEKYEYYLYHYRIMFNLSIFLTIIGSSLPTSIYMTQR--NPDEMSTAVSAYEEFINIWGVCHLMTYLDICGRIFFSLIITVPSLIIFIYVFFKNYEPKV------IFRSIYISSWVSLLFALISPITFVTNEYEKFTIENRSLTYCK---QGVELTVYNRCRLFELLCVHLIPLAIQIPCIVMIKKS----LTFANNSLLYEPIIFIVTNIPIAIIDTIEQ------MDEKWIHSSEFSNFYILFLTIYHLGYT 1013 F T LL +V ++++IP +Q + + ++R + + +ESD + + K + L+ YR++ + L +I S+LP I R +P+ + +WG CH++TY D R F + +I VP ++F F P+ +F I +S LF+ I P+ V + ++ E +T C G+ +Y ++ L +IP+A CI++ L + + YEP IF + ++PI + +T+ + +K I + FSNF ++ +YH+ ++ Sbjct: 42 FATCLLSGFFVVGILLIIPALMQTAAAYVWAAQVRKEASTRMNAQESDQRGLQKSTHSAKHVYINDGHRLSKQDRTLFLYRLLACCASLLVLIFSTLPQLIIGVARPLSPNTID----------KLWGWCHVLTYTDASTRCFATYLIVVPFGLLFWNFIFGEILPRPQLLTDNVFAVILRASVPIALFSFIIPVPMVAKRFVQYYDEG--MTKCNGSPDGLVFMIY-----YDYLVTRVIPVA----CIMVFSIGFVLWLPRKDYGVFYEPGIFTIFSVPIILCETVIHVYHRIGLVDKLIEPN-FSNFMLIAYALYHMSFS 342
BLAST of SMED30031472 vs. Planmine SMEST
Match: SMESG000016767.1 (SMESG000016767.1) HSP 1 Score: 669.848 bits (1727), Expect = 0.000e+0 Identity = 330/330 (100.00%), Postives = 330/330 (100.00%), Query Frame = 3 Query: 180 MLLVISCIVSLMMVIPITIQYIGSILDYRLRLQYQIEDTEESDNPIEKYEYYLYHYRIMFNLSIFLTIIGSSLPTSIYMTQRNPDEMSTAVSAYEEFINIWGVCHLMTYLDICGRIFFSLIITVPSLIIFIYVFFKNYEPKVIFRSIYISSWVSLLFALISPITFVTNEYEKFTIENRSLTYCKQGVELTVYNRCRLFELLCVHLIPLAIQIPCIVMIKKSLTFANNSLLYEPIIFIVTNIPIAIIDTIEQMDEKWIHSSEFSNFYILFLTIYHLGYTCMILVWPFQMIWMNKDENLQIKPEINDVYKILTNRKTIRKTVRRQFTAIKRK 1169 MLLVISCIVSLMMVIPITIQYIGSILDYRLRLQYQIEDTEESDNPIEKYEYYLYHYRIMFNLSIFLTIIGSSLPTSIYMTQRNPDEMSTAVSAYEEFINIWGVCHLMTYLDICGRIFFSLIITVPSLIIFIYVFFKNYEPKVIFRSIYISSWVSLLFALISPITFVTNEYEKFTIENRSLTYCKQGVELTVYNRCRLFELLCVHLIPLAIQIPCIVMIKKSLTFANNSLLYEPIIFIVTNIPIAIIDTIEQMDEKWIHSSEFSNFYILFLTIYHLGYTCMILVWPFQMIWMNKDENLQIKPEINDVYKILTNRKTIRKTVRRQFTAIKRK Sbjct: 1 MLLVISCIVSLMMVIPITIQYIGSILDYRLRLQYQIEDTEESDNPIEKYEYYLYHYRIMFNLSIFLTIIGSSLPTSIYMTQRNPDEMSTAVSAYEEFINIWGVCHLMTYLDICGRIFFSLIITVPSLIIFIYVFFKNYEPKVIFRSIYISSWVSLLFALISPITFVTNEYEKFTIENRSLTYCKQGVELTVYNRCRLFELLCVHLIPLAIQIPCIVMIKKSLTFANNSLLYEPIIFIVTNIPIAIIDTIEQMDEKWIHSSEFSNFYILFLTIYHLGYTCMILVWPFQMIWMNKDENLQIKPEINDVYKILTNRKTIRKTVRRQFTAIKRK 330
BLAST of SMED30031472 vs. Planmine SMEST
Match: SMESG000016767.1 (SMESG000016767.1) HSP 1 Score: 650.588 bits (1677), Expect = 0.000e+0 Identity = 319/319 (100.00%), Postives = 319/319 (100.00%), Query Frame = 3 Query: 213 MMVIPITIQYIGSILDYRLRLQYQIEDTEESDNPIEKYEYYLYHYRIMFNLSIFLTIIGSSLPTSIYMTQRNPDEMSTAVSAYEEFINIWGVCHLMTYLDICGRIFFSLIITVPSLIIFIYVFFKNYEPKVIFRSIYISSWVSLLFALISPITFVTNEYEKFTIENRSLTYCKQGVELTVYNRCRLFELLCVHLIPLAIQIPCIVMIKKSLTFANNSLLYEPIIFIVTNIPIAIIDTIEQMDEKWIHSSEFSNFYILFLTIYHLGYTCMILVWPFQMIWMNKDENLQIKPEINDVYKILTNRKTIRKTVRRQFTAIKRK 1169 MMVIPITIQYIGSILDYRLRLQYQIEDTEESDNPIEKYEYYLYHYRIMFNLSIFLTIIGSSLPTSIYMTQRNPDEMSTAVSAYEEFINIWGVCHLMTYLDICGRIFFSLIITVPSLIIFIYVFFKNYEPKVIFRSIYISSWVSLLFALISPITFVTNEYEKFTIENRSLTYCKQGVELTVYNRCRLFELLCVHLIPLAIQIPCIVMIKKSLTFANNSLLYEPIIFIVTNIPIAIIDTIEQMDEKWIHSSEFSNFYILFLTIYHLGYTCMILVWPFQMIWMNKDENLQIKPEINDVYKILTNRKTIRKTVRRQFTAIKRK Sbjct: 1 MMVIPITIQYIGSILDYRLRLQYQIEDTEESDNPIEKYEYYLYHYRIMFNLSIFLTIIGSSLPTSIYMTQRNPDEMSTAVSAYEEFINIWGVCHLMTYLDICGRIFFSLIITVPSLIIFIYVFFKNYEPKVIFRSIYISSWVSLLFALISPITFVTNEYEKFTIENRSLTYCKQGVELTVYNRCRLFELLCVHLIPLAIQIPCIVMIKKSLTFANNSLLYEPIIFIVTNIPIAIIDTIEQMDEKWIHSSEFSNFYILFLTIYHLGYTCMILVWPFQMIWMNKDENLQIKPEINDVYKILTNRKTIRKTVRRQFTAIKRK 319 The following BLAST results are available for this feature:
BLAST of SMED30031472 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30031472 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30031472 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30031472 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30031472 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30031472 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30031472 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30031472 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30031472 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30031472 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30031472 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30031472 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30031472 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30031472 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30031472 ID=SMED30031472|Name=SMED30031472|organism=Schmidtea mediterranea sexual|type=transcript|length=1352bpback to top protein sequence of SMED30031472-orf-1 >SMED30031472-orf-1 ID=SMED30031472-orf-1|Name=SMED30031472-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=357bp MNFSTEQSDLHLLTDKSNVYLIVFVTMLLVISCIVSLMMVIPITIQYIGSback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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