SMED30031471
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30031471 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 10Note: Hover over icons to view figure legend
Homology
BLAST of SMED30031471 vs. Planmine SMEST
Match: SMESG000050533.1 (SMESG000050533.1) HSP 1 Score: 968.763 bits (2503), Expect = 0.000e+0 Identity = 515/516 (99.81%), Postives = 515/516 (99.81%), Query Frame = 3 Query: 1434 MTNKRKGEIITSSPIKITKLDSSYSFEIVNNPIFTPHKSSPDGLINLFALVLNLVPLKLPIDNGVCTILTNSCVTIQEGIQEPALRNIKINHESCKANGVSEIIANIYSIFNEPEKEVLNISFMKFIWVKYFPQELSNFNLMILFYRFVKLLMLYTMFNKSLVTXXXXXXXXXXXXXXXNFNFKSSVSTFKKRQLSCFSHKFDKYFKEHDQLHXXXXXXXXXXXXXXXXXXXXXXXXXXXXEFTVGPLLNYFISSINSIHNKMEPFQIEVVYGAIRTLRFCLRKINLKLVQTSFTKPYLFDFKTKNMDHHCTEVKKEINWLCKELKYYQCVLFTDKHYDFINCILDFSWRLECTIKVNSDPLQPKPICEPLVFHNPMQFVQTFTTFQCPIFIQYHPESELEYLNTCLKTKWIGILHEAFIGSLPEPCGARIIKEINVLLPPTWGLFTFNEAIIESSKMYYERFQKLAETDPSGWYTKFSKTKHCAMNWLIQYQLPVDGLEVLHDYQYVLESKYKYS 2981 MTNKRKGEIITSSPIKITKLDSSYSFEIVNNPIFTPHKSSPDGLINLFALVLNLVPLKLPIDNGVCTILTNSCVTIQEGIQEPALRNIKINHESCKANGVSEIIANIYSIFNEPEKEVLNISFMKFIWVKYFPQELSNFNLMILFYRFVKLLMLYTMFNKSLVTKKLVIDVIEKIDVIVNFNFKSSVSTFKKRQLSCFSHKFDKYFKEHDQLHVLYSKLSNSKSLVVNSKKLDITIKKKKIEFTVGPLLNYFISSINSIHNKMEPFQIEVVYGAIRTLRFCLRKINLKLVQTSFTKPYLFDFKTKNMDHHCTEVKKEINWLCKELKYYQCVLFTDKHYDFINCILDFSWRLECTIKVNSDPLQPKPICEPLVFHNPMQFVQTFT FQCPIFIQYHPESELEYLNTCLKTKWIGILHEAFIGSLPEPCGARIIKEINVLLPPTWGLFTFNEAIIESSKMYYERFQKLAETDPSGWYTKFSKTKHCAMNWLIQYQLPVDGLEVLHDYQYVLESKYKYS Sbjct: 1 MTNKRKGEIITSSPIKITKLDSSYSFEIVNNPIFTPHKSSPDGLINLFALVLNLVPLKLPIDNGVCTILTNSCVTIQEGIQEPALRNIKINHESCKANGVSEIIANIYSIFNEPEKEVLNISFMKFIWVKYFPQELSNFNLMILFYRFVKLLMLYTMFNKSLVTKKLVIDVIEKIDVIVNFNFKSSVSTFKKRQLSCFSHKFDKYFKEHDQLHVLYSKLSNSKSLVVNSKKLDITIKKKKIEFTVGPLLNYFISSINSIHNKMEPFQIEVVYGAIRTLRFCLRKINLKLVQTSFTKPYLFDFKTKNMDHHCTEVKKEINWLCKELKYYQCVLFTDKHYDFINCILDFSWRLECTIKVNSDPLQPKPICEPLVFHNPMQFVQTFTAFQCPIFIQYHPESELEYLNTCLKTKWIGILHEAFIGSLPEPCGARIIKEINVLLPPTWGLFTFNEAIIESSKMYYERFQKLAETDPSGWYTKFSKTKHCAMNWLIQYQLPVDGLEVLHDYQYVLESKYKYS 516
BLAST of SMED30031471 vs. Planmine SMEST
Match: SMESG000050535.1 (SMESG000050535.1) HSP 1 Score: 532.332 bits (1370), Expect = 0.000e+0 Identity = 258/258 (100.00%), Postives = 258/258 (100.00%), Query Frame = 3 Query: 120 MIICSPSLAISNDTSGDYHTFIPDKYELIFNNSMCDIAFIVTHQPLMSKLDKLQNYFQSELKFSTYCTYIEDNESLKFYLRDLKTHDVSYALLLHDSFENRNFYDLYVLHKDEVLPNITLEKVISLVRSDFDNMMSKVKILKNIPSKWPHSVKPEDLEYSKINDKLRYYFCLLSDSRTLSIEELNYVNDYLVTRKELLQISKTKNIFEKDNFIQPSKFVFKGPNRTGTVIKLSRAEDYDSEPSLKYTTLDVGFAGSLS 893 MIICSPSLAISNDTSGDYHTFIPDKYELIFNNSMCDIAFIVTHQPLMSKLDKLQNYFQSELKFSTYCTYIEDNESLKFYLRDLKTHDVSYALLLHDSFENRNFYDLYVLHKDEVLPNITLEKVISLVRSDFDNMMSKVKILKNIPSKWPHSVKPEDLEYSKINDKLRYYFCLLSDSRTLSIEELNYVNDYLVTRKELLQISKTKNIFEKDNFIQPSKFVFKGPNRTGTVIKLSRAEDYDSEPSLKYTTLDVGFAGSLS Sbjct: 1 MIICSPSLAISNDTSGDYHTFIPDKYELIFNNSMCDIAFIVTHQPLMSKLDKLQNYFQSELKFSTYCTYIEDNESLKFYLRDLKTHDVSYALLLHDSFENRNFYDLYVLHKDEVLPNITLEKVISLVRSDFDNMMSKVKILKNIPSKWPHSVKPEDLEYSKINDKLRYYFCLLSDSRTLSIEELNYVNDYLVTRKELLQISKTKNIFEKDNFIQPSKFVFKGPNRTGTVIKLSRAEDYDSEPSLKYTTLDVGFAGSLS 258 HSP 2 Score: 131.339 bits (329), Expect = 0.000e+0 Identity = 96/99 (96.97%), Postives = 98/99 (98.99%), Query Frame = 1 Query: 895 RLHHQVSSHSKSKRLNRHPFRMRSYKAQMYSLLKDXXXXXXXXXKSLXXXXXXXXXXXXXXXXXXXIIEIISLLSGRFVLRKSNATNSYPISRKSIKFW 1191 RLHHQVSSHSKSKRLNRHPFRMRSYKAQMYSLLKDPRIIIPIRRKSLRNP+RTNQKRNK+N NGNQIIEIISLLSGRFVLRKSNATNSYPISRKSIKFW Sbjct: 259 RLHHQVSSHSKSKRLNRHPFRMRSYKAQMYSLLKDPRIIIPIRRKSLRNPDRTNQKRNKKNRNGNQIIEIISLLSGRFVLRKSNATNSYPISRKSIKFW 357
BLAST of SMED30031471 vs. Planmine SMEST
Match: SMESG000050537.1 (SMESG000050537.1) HSP 1 Score: 345.51 bits (885), Expect = 2.293e-103 Identity = 228/632 (36.08%), Postives = 367/632 (58.07%), Query Frame = 3 Query: 1134 AEIKRNKFVSNLEKIDKVLVKTAQMSKLSINYLKAT-LSNNFSQKVDRSVTIKYFKSSYYKNRMNYVAPMLVVRPLVPLNQNKKLVDLTSASDGDNQNSDKMTNKRKGEIITSSPI-----KITKLDSSYSFEI-------VNNPIFTPH----KSSPDGLINLFALVLNLVPLKLPIDNGVCTILTNSCVTIQEGIQEPALRNIKINHESCKANGVSEIIANIYSIFNEPEKEVLNISFMKFIWVKYFPQELSNFNLMILFYRFVKLLMLYTMFNKSLVTXXXXXXXXXXXXXXXNFNFKSSVSTFKKRQLSCFSHKFDKYFKEHDQLHXXXXXXXXXXXXXXXXXXXXXXXXXXXXEFTVGPLLNYFISSINSIHNKMEPFQIEVVYGAIRTLRFCLRKI-NLKLVQTSFTKPYLFDFKTKNMDHHCTEVKKEINWLCKELKYYQCVLFTDKHYDFINCILDFSWRLECTIKVNSDPLQPKPICEPLVFHNPMQFVQTFTTFQCPIFIQYHPESELEYLNTCLKTKWIGILHEAFIGSLPEPCGARIIKEINVLLPPTWGLFTFNEAIIESSKMYYERFQKLAETDPSGWYTKFSKTKHCAMNWLIQYQLPVD-GLEVLHDYQYVLESKY 2972 A+I N F SNL++I + + Q+ S ++ + + K + S +Y KS+Y+ N+ + ++ ++ L+S S D + E ITSS + K+ LD S S + + P T K++PD L+N+F + + +P LP DN + I+ N C IQ GI EP+L+ +KI++ESCK NG +EI+ +IY I+ EK V +I +++IW KYFP LSNF+L+++FY+F+K+L+++ +FN +++ + +EK+++I NF+F + + K+ L+C S F + H LH Y L + + +++KKLD IKK+KIEF + PLL YF++++ SIH +M+ Q E+VY AIRTLR+CLR I + T F P F +++ ++ +++ EI WL E+KYYQCVLFT+KHYDFINC+LDFS+ +E I +SD + + LV + QF+++F F+ + +QYH +SE+ L LK W+ IL +AF G + G RI+KEIN LLP WGL TF+EA+++++K Y + K+A+ +P+GW+ FSK K + WL QL ++ +E L + +L +++ Sbjct: 302 AKIITNTFGSNLQQIKSITIARGQLKPFSYEVIQGGGILKTEAIKKNDSQREEYSKSNYFINQNLW-------------REDDDIISLSSGSCTPPIEYDLIPISPPDEQITSSKVDLGKRKLATLDLSESNSLPKLHKIDLRKPEITASTLVIKNTPDVLVNMFRFIAHAMPYDLPHDNAILNIMINYCNDIQNGIMEPSLKIVKIDYESCKINGANEIVKSIYGIYEVAEKNVEDIVDLQYIWKKYFPDHLSNFSLLVVFYKFIKMLIIFRLFNSQSDRLQIISNTVEKLNLIANFDFYLPLLS-DKQHLNCISELFGNTLESHSDLHTKYFDLPHISEIAISTKKLDNVIKKRKIEFVLSPLLKYFVNTLKSIHQQMKLAQTELVYKAIRTLRYCLRVIYPINSPTTVFIVPKTF---SESGNNGYKDIQDEIKWLLDEIKYYQCVLFTEKHYDFINCVLDFSFVVEGII--DSDSSTSRSNTDSLVVLDTDQFIESFAAFEG-MNLQYHADSEISCLKHFLKESWVQILQKAFRGLSTDGKGKRIVKEINNLLPELWGLKTFDEAVVDAAKCNYTEWTKIAQNNPTGWHESFSKVKFAGIVWLNTNQLSLNKAVEELQRCENMLRNEF 913 HSP 2 Score: 116.316 bits (290), Expect = 3.584e-26 Identity = 71/176 (40.34%), Postives = 109/176 (61.93%), Query Frame = 3 Query: 198 ELIFNNSMCDIAFIVTHQPLMSKLDKLQNYFQSELKFSTYCTYIEDNESLKFYLRDLKTHDVSYALLLHDSFENRNFYDLYVLHK-DEVLPNITLEKVISLVRSDFDNMMSKVKILKNIPSKWPHSVKPEDLEYSKINDKLRYYFCLLSDSRTLSIEELNYVNDYLVTRKELLQIS 722 ELIFN D+A I+TH L+S + +LQN+F + KF+T+ YI D LKF +RDLK H ++YA+++++ + DLYVL+ E+ + L +V LVR D N ++K L PSK+PH P++ E+ +N++ RY CLLSD+R L ++EL +N +L RK ++ S Sbjct: 40 ELIFNKPTFDMALIITHTSLISDICELQNFFHKKHKFTTHVIYIPDVSKLKFCIRDLKLHRITYAIVINEYGLIQGKMDLYVLYPIIEIQKRMNLSEVDDLVRDDSYNTSIQIKSLMEKPSKYPH---PKN-EF-HVNNETRYQLCLLSDNRMLKMQELQNLNRFLCQRKAEMERS 210
BLAST of SMED30031471 vs. Planmine SMEST
Match: SMESG000050537.1 (SMESG000050537.1) HSP 1 Score: 342.043 bits (876), Expect = 2.534e-102 Identity = 221/618 (35.76%), Postives = 360/618 (58.25%), Query Frame = 3 Query: 1134 AEIKRNKFVSNLEKIDKVLVKTAQMSKLSINYLKAT-LSNNFSQKVDRSVTIKYFKSSYYKNRMNYVAPMLVVRPLVPLNQN--KKLVDLTSASDGDNQNSDKMTNKRKGEIITSSPIKITKLDSSYSFEIVNNPIFTPHKSSPDGLINLFALVLNLVPLKLPIDNGVCTILTNSCVTIQEGIQEPALRNIKINHESCKANGVSEIIANIYSIFNEPEKEVLNISFMKFIWVKYFPQELSNFNLMILFYRFVKLLMLYTMFNKSLVTXXXXXXXXXXXXXXXNFNFKSSVSTFKKRQLSCFSHKFDKYFKEHDQLHXXXXXXXXXXXXXXXXXXXXXXXXXXXXEFTVGPLLNYFISSINSIHNKMEPFQIEVVYGAIRTLRFCLRKI-NLKLVQTSFTKPYLFDFKTKNMDHHCTEVKKEINWLCKELKYYQCVLFTDKHYDFINCILDFSWRLECTIKVNSDPLQPKPICEPLVFHNPMQFVQTFTTFQCPIFIQYHPESELEYLNTCLKTKWIGILHEAFIGSLPEPCGARIIKEINVLLPPTWGLFTFNEAIIESSKMYYERFQKLAETDPSGWYTKFSKTKHCAMNWLIQYQLPVD-GLEVLHDYQYVLESKY 2972 A+I N F SNL++I + + Q+ S ++ + + K + S +Y KS+Y+ +NQN ++ D+ S S G + + + I + K + + S ++ K++PD L+N+F + + +P LP DN + I+ N C IQ GI EP+L+ +KI++ESCK NG +EI+ +IY I+ EK V +I +++IW KYFP LSNF+L+++FY+F+K+L+++ +FN +++ + +EK+++I NF+F + + K+ L+C S F + H LH Y L + + +++KKLD IKK+KIEF + PLL YF++++ SIH +M+ Q E+VY AIRTLR+CLR I + T F P F +++ ++ +++ EI WL E+KYYQCVLFT+KHYDFINC+LDFS+ +E I +SD + + LV + QF+++F F+ + +QYH +SE+ L LK W+ IL +AF G + G RI+KEIN LLP WGL TF+EA+++++K Y + K+A+ +P+GW+ FSK K + WL QL ++ +E L + +L +++ Sbjct: 302 AKIITNTFGSNLQQIKSITIARGQLKPFSYEVIQGGGILKTEAIKKNDSQREEYSKSNYF------------------INQNLWREDDDIISLSSGCKRKLATLDLSESNSLPKLHKIDLRKPEITASTLVI--------KNTPDVLVNMFRFIAHAMPYDLPHDNAILNIMINYCNDIQNGIMEPSLKIVKIDYESCKINGANEIVKSIYGIYEVAEKNVEDIVDLQYIWKKYFPDHLSNFSLLVVFYKFIKMLIIFRLFNSQSDRLQIISNTVEKLNLIANFDFYLPLLS-DKQHLNCISELFGNTLESHSDLHTKYFDLPHISEIAISTKKLDNVIKKRKIEFVLSPLLKYFVNTLKSIHQQMKLAQTELVYKAIRTLRYCLRVIYPINSPTTVFIVPKTF---SESGNNGYKDIQDEIKWLLDEIKYYQCVLFTEKHYDFINCVLDFSFVVEGII--DSDSSTSRSNTDSLVVLDTDQFIESFAAFEG-MNLQYHADSEISCLKHFLKESWVQILQKAFRGLSTDGKGKRIVKEINNLLPELWGLKTFDEAVVDAAKCNYTEWTKIAQNNPTGWHESFSKVKFAGIVWLNTNQLSLNKAVEELQRCENMLRNEF 886 HSP 2 Score: 116.316 bits (290), Expect = 3.668e-26 Identity = 71/176 (40.34%), Postives = 109/176 (61.93%), Query Frame = 3 Query: 198 ELIFNNSMCDIAFIVTHQPLMSKLDKLQNYFQSELKFSTYCTYIEDNESLKFYLRDLKTHDVSYALLLHDSFENRNFYDLYVLHK-DEVLPNITLEKVISLVRSDFDNMMSKVKILKNIPSKWPHSVKPEDLEYSKINDKLRYYFCLLSDSRTLSIEELNYVNDYLVTRKELLQIS 722 ELIFN D+A I+TH L+S + +LQN+F + KF+T+ YI D LKF +RDLK H ++YA+++++ + DLYVL+ E+ + L +V LVR D N ++K L PSK+PH P++ E+ +N++ RY CLLSD+R L ++EL +N +L RK ++ S Sbjct: 40 ELIFNKPTFDMALIITHTSLISDICELQNFFHKKHKFTTHVIYIPDVSKLKFCIRDLKLHRITYAIVINEYGLIQGKMDLYVLYPIIEIQKRMNLSEVDDLVRDDSYNTSIQIKSLMEKPSKYPH---PKN-EF-HVNNETRYQLCLLSDNRMLKMQELQNLNRFLCQRKAEMERS 210
BLAST of SMED30031471 vs. Planmine SMEST
Match: SMESG000010804.1 (SMESG000010804.1) HSP 1 Score: 60.4622 bits (145), Expect = 5.940e-9 Identity = 49/176 (27.84%), Postives = 79/176 (44.89%), Query Frame = 3 Query: 225 DIAFIVTHQPLMSKLDKLQNYFQSELKFSTYCTYIEDNESLKFYLRDLKTHDVSYALLL------HDSFENRNFYDLYVLHKDEVLPNITLEKVISLVRSDFDNMMSKVKILKNI-------PSKWPHSVKPEDLEYSKINDKLRYYFCLLSDSRTLSIEELNYVNDYLVTRKELL 713 D A IV L+S + +++ Q ++ +T+ +++ + DL V YA L+ H S R Y H+ N+ L ++ V +F+ V+ LK I PS+ P + +DK+ YY LL+DSR L+IEELN V D++ R + L Sbjct: 228 DAAIIVMSSELLSYAETIESRLQRQIALTTHIIVLKEESHANACVEDLNNKGVLYAFLVNSLNEQHFSCTLRILYGTPQEHR-----NMPLNNALTFVARNFERYQKHVRDLKTIAPKPTFDPSRMPPG-------FIAPDDKIIYYLHLLADSRMLTIEELNEVIDFVQKRMQAL 391 The following BLAST results are available for this feature:
BLAST of SMED30031471 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30031471 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30031471 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30031471 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30031471 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30031471 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30031471 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30031471 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30031471 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30031471 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30031471 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30031471 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30031471 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30031471 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30031471 ID=SMED30031471|Name=SMED30031471|organism=Schmidtea mediterranea sexual|type=transcript|length=3055bpback to top protein sequence of SMED30031471-orf-1 >SMED30031471-orf-1 ID=SMED30031471-orf-1|Name=SMED30031471-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=955bp MIICSPSLAISNDTSGDYHTFIPDKYELIFNNSMCDIAFIVTHQPLMSKLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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