ATP synthase subunit

Overview
NameATP synthase subunit
Smed IDSMED30031423
Length (bp)472
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of ATP synthase subunit (SMED30031423) t-SNE clustered cells

Violin plots show distribution of expression levels for ATP synthase subunit (SMED30031423) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of ATP synthase subunit (SMED30031423) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for ATP synthase subunit (SMED30031423) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30031423

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30031423SMESG000021514.1 dd_Smed_v4_428_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30031423SMESG000021514.1 dd_Smed_v4_428_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30031423SMESG000021514.1 dd_Smed_v4_428_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30031423SMESG000021514.1 dd_Smed_v4_428_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30031423SMESG000021514.1 dd_Smed_v4_428_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031423SMESG000021514.1 dd_Smed_v4_428_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031423SMESG000021514.1 Contig51129newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031423SMESG000021514.1 Contig51129uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031423SMESG000021514.1 Contig1376GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30031423SMESG000026697.1 SMESG000021514.1 OX_Smed_1.0.08913ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of ATP synthase subunit vs. Ensembl Human
Match: ATP5MG (ATP synthase membrane subunit g [Source:HGNC Symbol;Acc:HGNC:14247])

HSP 1 Score: 92.0485 bits (227), Expect = 1.964e-24
Identity = 44/94 (46.81%), Postives = 60/94 (63.83%), Query Frame = 2 
Query:   71 VQKLQKLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYS 352
            V+K   L    + Y+KPRL  F  YAKVE+ PPTP++IP A   L K+V S  T  +  + +KEA++NG+V  EV+ WF+ GEIIG+R   GY 
Sbjct:    9 VEKTPALVNAAVTYSKPRLATFWYYAKVELVPPTPAEIPRAIQSLKKIVNSAQTGSFKQLTVKEAVLNGLVATEVLMWFYVGEIIGKRGIIGYD 102
[Close]
BLAST of ATP synthase subunit vs. Ensembl Human
Match: ATP5MGL (ATP synthase membrane subunit g like [Source:HGNC Symbol;Acc:HGNC:13213])

HSP 1 Score: 77.0258 bits (188), Expect = 1.398e-18
Identity = 39/92 (42.39%), Postives = 53/92 (57.61%), Query Frame = 2 
Query:   71 VQKLQKLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFG 346
            V+K   L    + Y KPRL  F  Y  VE+ PPTP++IP A   L K+V S  T  +  + +KEA++NG+V  EV  WF+  EI G+R   G
Sbjct:    9 VEKTPALVNAAVTYLKPRLAAFWYYTTVELVPPTPAEIPRAIQSLKKIVSSAQTGSFKQLTVKEALLNGLVATEVSTWFYVREITGKRGIIG 100
[Close]
BLAST of ATP synthase subunit vs. Ensembl Human
Match: ATP5MG (ATP synthase membrane subunit g [Source:HGNC Symbol;Acc:HGNC:14247])

HSP 1 Score: 49.2914 bits (116), Expect = 4.369e-8
Identity = 27/64 (42.19%), Postives = 38/64 (59.38%), Query Frame = 2 
Query:   71 VQKLQKLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKE 262
            V+K   L    + Y+KPRL  F  YAKVE+ PPTP++IP A   L K+V S  T  +  + +K+
Sbjct:    9 VEKTPALVNAAVTYSKPRLATFWYYAKVELVPPTPAEIPRAIQSLKKIVNSAQTGSFKQLTVKK 72
[Close]
BLAST of ATP synthase subunit vs. Ensembl Human
Match: AP001267.5 (novel protein)

HSP 1 Score: 48.521 bits (114), Expect = 1.048e-7
Identity = 27/63 (42.86%), Postives = 37/63 (58.73%), Query Frame = 2 
Query:   71 VQKLQKLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMK 259
            V+K   L    + Y+KPRL  F  YAKVE+ PPTP++IP A   L K+V S  T  +  + +K
Sbjct:    9 VEKTPALVNAAVTYSKPRLATFWYYAKVELVPPTPAEIPRAIQSLKKIVNSAQTGSFKQLTVK 71
[Close]
BLAST of ATP synthase subunit vs. Ensembl Celegans
Match: asg-1 (Probable ATP synthase subunit g 1, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:P90921])

HSP 1 Score: 66.2402 bits (160), Expect = 1.670e-14
Identity = 35/83 (42.17%), Postives = 48/83 (57.83%), Query Frame = 2 
Query:  122 RLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYSRIPGCY 370
            RL    +  K E+ PP  +DIP      AKL K   T ++ ++ +KE+++   V  EVVFWFF GE+IGRR  FGY  +P  Y
Sbjct:   30 RLAILKAVGKHELAPPRSADIPAIKADWAKLQKFIETKQYVNLSIKESLVYSAVALEVVFWFFVGEMIGRRYIFGYI-VPANY 111
[Close]
BLAST of ATP synthase subunit vs. Ensembl Celegans
Match: asg-2 (Probable ATP synthase subunit g 2, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:Q18803])

HSP 1 Score: 57.3806 bits (137), Expect = 4.151e-11
Identity = 30/75 (40.00%), Postives = 42/75 (56.00%), Query Frame = 2 
Query:  146 AKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYSRIPGCY 370
             K E+ PP  +D P      AK+     T  + ++ ++E ++   VT EVVFWFF GE+IGRR  FGY  +P  Y
Sbjct:   38 GKHELAPPRQADWPAIKADWAKVQSFIQTGGYKNLSIREGLVYTAVTLEVVFWFFVGEMIGRRYIFGYL-VPADY 111
[Close]
BLAST of ATP synthase subunit vs. Ensembl Fly
Match: ATPsynG (gene:FBgn0010612 transcript:FBtr0080167)

HSP 1 Score: 92.0485 bits (227), Expect = 8.604e-25
Identity = 40/83 (48.19%), Postives = 58/83 (69.88%), Query Frame = 2 
Query:  104 LDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYS 352
            L  A+P+LD F+ YAKVE+ PPTP+DIP    GL  ++K   T  + ++ ++EA +N +VTAEV+FWF+ GE IG+R   GY+
Sbjct:   16 LTQARPQLDVFLKYAKVELTPPTPADIPAIRQGLGNIIKGAKTGAYKNLTVREAWLNTLVTAEVIFWFYIGECIGKRHIVGYN 98
[Close]
BLAST of ATP synthase subunit vs. Ensembl Fly
Match: ATPsynG (gene:FBgn0010612 transcript:FBtr0110872)

HSP 1 Score: 92.0485 bits (227), Expect = 8.604e-25
Identity = 40/83 (48.19%), Postives = 58/83 (69.88%), Query Frame = 2 
Query:  104 LDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYS 352
            L  A+P+LD F+ YAKVE+ PPTP+DIP    GL  ++K   T  + ++ ++EA +N +VTAEV+FWF+ GE IG+R   GY+
Sbjct:   16 LTQARPQLDVFLKYAKVELTPPTPADIPAIRQGLGNIIKGAKTGAYKNLTVREAWLNTLVTAEVIFWFYIGECIGKRHIVGYN 98
[Close]
BLAST of ATP synthase subunit vs. Ensembl Fly
Match: ATPsynG (gene:FBgn0010612 transcript:FBtr0305994)

HSP 1 Score: 92.0485 bits (227), Expect = 8.604e-25
Identity = 40/83 (48.19%), Postives = 58/83 (69.88%), Query Frame = 2 
Query:  104 LDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYS 352
            L  A+P+LD F+ YAKVE+ PPTP+DIP    GL  ++K   T  + ++ ++EA +N +VTAEV+FWF+ GE IG+R   GY+
Sbjct:   16 LTQARPQLDVFLKYAKVELTPPTPADIPAIRQGLGNIIKGAKTGAYKNLTVREAWLNTLVTAEVIFWFYIGECIGKRHIVGYN 98
[Close]
BLAST of ATP synthase subunit vs. Ensembl Fly
Match: ATPsynGL (gene:FBgn0031941 transcript:FBtr0079481)

HSP 1 Score: 63.929 bits (154), Expect = 8.950e-14
Identity = 36/94 (38.30%), Postives = 51/94 (54.26%), Query Frame = 2 
Query:  113 AKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWAS---------------MPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGY 349
            A+P+LD+F  YAKVE+ PP P+D         KL ++  + K AS               + + EA +N +VT EV+ WF+ GE+IGRR   GY
Sbjct:   19 ARPQLDEFWKYAKVELSPPLPAD-------FQKLKQTAESAKLASKKDMKGQLKKSGLSQVTVAEAWLNVLVTVEVITWFYMGEVIGRRHLVGY 105
[Close]
BLAST of ATP synthase subunit vs. Ensembl Zebrafish
Match: atp5l (ATP synthase, H+ transporting, mitochondrial F0 complex, subunit g [Source:ZFIN;Acc:ZDB-GENE-030131-5177])

HSP 1 Score: 82.0333 bits (201), Expect = 1.001e-20
Identity = 38/94 (40.43%), Postives = 59/94 (62.77%), Query Frame = 2 
Query:   71 VQKLQKLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYS 352
            V K+  L    ++Y+KPRL  F  YA+VE+ PPTP++IP A  G   ++K+  + +     +++A+ NG+V  EV+ WF+ GEIIG+R   GY 
Sbjct:    9 VAKVPTLVGAAVNYSKPRLATFWYYARVELVPPTPAEIPKAISGFQDMLKAFQSGRVGQTTVRDAVRNGLVATEVLMWFYIGEIIGKRGLIGYD 102
[Close]
BLAST of ATP synthase subunit vs. Ensembl Xenopus
Match: xkrx (XK related X-linked [Source:Xenbase;Acc:XB-GENE-957186])

HSP 1 Score: 77.7962 bits (190), Expect = 9.277e-19
Identity = 36/96 (37.50%), Postives = 59/96 (61.46%), Query Frame = 2 
Query:   65 AYVQKLQKLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYS 352
            A   K  +L    + Y++PRL  F  YA+VE+ PP+P+++P A   +  LVKS  + + + + ++EA+ NG+V  EV+ WF+ GE IG+    GY+
Sbjct:   11 AVTTKTPQLIGAAVGYSRPRLATFWYYARVELTPPSPAEVPKAIESIKGLVKSFQSGRLSELTVREALRNGLVATEVLMWFYIGECIGKGGLVGYN 106
[Close]
BLAST of ATP synthase subunit vs. Ensembl Mouse
Match: Atp5l (ATP synthase, H+ transporting, mitochondrial F0 complex, subunit G [Source:MGI Symbol;Acc:MGI:1351597])

HSP 1 Score: 88.1965 bits (217), Expect = 5.039e-23
Identity = 41/101 (40.59%), Postives = 63/101 (62.38%), Query Frame = 2 
Query:   50 SKY*MAYVQKLQKLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYS 352
            +K+   + +K   +    + Y+KPRL  F  YAKVE+ PPTP++IP A   + K+++S  T  +  + +KEA++NG+V  EV  WF+ GEIIG+R   GY 
Sbjct:    2 AKFIRNFAEKAPSMVAAAVTYSKPRLATFWHYAKVELVPPTPAEIPTAIQSVKKIIQSAKTGSFKHLTVKEAVLNGLVATEVWMWFYIGEIIGKRGIVGYD 102
[Close]
BLAST of ATP synthase subunit vs. Ensembl Mouse
Match: Gm5426 (predicted pseudogene 5426 [Source:MGI Symbol;Acc:MGI:3646788])

HSP 1 Score: 85.1149 bits (209), Expect = 8.976e-22
Identity = 39/101 (38.61%), Postives = 62/101 (61.39%), Query Frame = 2 
Query:   50 SKY*MAYVQKLQKLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYS 352
            +K+   + +K   +    + Y KP+L  F  YAKVE+ PPTP +IP A   + K+++S  T  +  + +KEA++NG+V  EV+ WF+ GEI+G+R   GY 
Sbjct:    2 AKFIHNFSEKALSMVAAAVTYLKPQLVTFWHYAKVELVPPTPGEIPTAIQNVKKIIQSAKTGSFKHLTVKEAVLNGLVATEVLMWFYIGEIMGKRGIVGYD 102
[Close]
BLAST of ATP synthase subunit vs. UniProt/SwissProt
Match: sp|Q5RFH0|ATP5L_PONAB (ATP synthase subunit g, mitochondrial OS=Pongo abelii OX=9601 GN=ATP5MG PE=3 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 9.977e-25
Identity = 45/94 (47.87%), Postives = 61/94 (64.89%), Query Frame = 2 
Query:   71 VQKLQKLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYS 352
            V+K   L    + Y+KPRL  F  YAKVE+ PPTP++IP A  GL K+V S  T  +  + +KEA++NG+V  EV+ WF+ GEIIG+R   GY 
Sbjct:    9 VEKTPALVNAAVTYSKPRLATFWYYAKVELVPPTPAEIPRAIQGLKKIVNSAQTGSFKQLTVKEAVLNGLVATEVLMWFYVGEIIGKRGIIGYD 102
[Close]
BLAST of ATP synthase subunit vs. UniProt/SwissProt
Match: sp|O75964|ATP5L_HUMAN (ATP synthase subunit g, mitochondrial OS=Homo sapiens OX=9606 GN=ATP5MG PE=1 SV=3)

HSP 1 Score: 92.0485 bits (227), Expect = 9.431e-24
Identity = 44/94 (46.81%), Postives = 60/94 (63.83%), Query Frame = 2 
Query:   71 VQKLQKLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYS 352
            V+K   L    + Y+KPRL  F  YAKVE+ PPTP++IP A   L K+V S  T  +  + +KEA++NG+V  EV+ WF+ GEIIG+R   GY 
Sbjct:    9 VEKTPALVNAAVTYSKPRLATFWYYAKVELVPPTPAEIPRAIQSLKKIVNSAQTGSFKQLTVKEAVLNGLVATEVLMWFYVGEIIGKRGIIGYD 102
[Close]
BLAST of ATP synthase subunit vs. UniProt/SwissProt
Match: sp|Q9CPQ8|ATP5L_MOUSE (ATP synthase subunit g, mitochondrial OS=Mus musculus OX=10090 GN=Atp5mg PE=1 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 3.524e-22
Identity = 41/101 (40.59%), Postives = 63/101 (62.38%), Query Frame = 2 
Query:   50 SKY*MAYVQKLQKLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYS 352
            +K+   + +K   +    + Y+KPRL  F  YAKVE+ PPTP++IP A   + K+++S  T  +  + +KEA++NG+V  EV  WF+ GEIIG+R   GY 
Sbjct:    2 AKFIRNFAEKAPSMVAAAVTYSKPRLATFWHYAKVELVPPTPAEIPTAIQSVKKIIQSAKTGSFKHLTVKEAVLNGLVATEVWMWFYIGEIIGKRGIVGYD 102
[Close]
BLAST of ATP synthase subunit vs. UniProt/SwissProt
Match: sp|Q28852|ATP5L_BOVIN (ATP synthase subunit g, mitochondrial OS=Bos taurus OX=9913 GN=ATP5MG PE=1 SV=3)

HSP 1 Score: 88.1965 bits (217), Expect = 3.761e-22
Identity = 42/93 (45.16%), Postives = 58/93 (62.37%), Query Frame = 2 
Query:   74 QKLQKLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYS 352
            +K   L    + Y+KPRL  F  YAKVE+ PPTP++IP A   L K++ S  T  +  + +KEA++NG+V  EV  WF+ GEIIG+R   GY 
Sbjct:   10 EKAPALVNAAVTYSKPRLATFWYYAKVELVPPTPAEIPTAIQSLKKIINSAKTGSFKQLTVKEALLNGLVATEVWMWFYVGEIIGKRGIIGYD 102
[Close]
BLAST of ATP synthase subunit vs. UniProt/SwissProt
Match: sp|Q6PDU7|ATP5L_RAT (ATP synthase subunit g, mitochondrial OS=Rattus norvegicus OX=10116 GN=Atp5mg PE=1 SV=2)

HSP 1 Score: 80.4925 bits (197), Expect = 4.075e-19
Identity = 36/83 (43.37%), Postives = 52/83 (62.65%), Query Frame = 2 
Query:  104 LDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYS 352
            + Y+KPRL  F  YA+VE+ PPT  +IP A   +  ++ S  T  +  + +KEA++NG+V  EV  WF+ GEIIG+R   GY 
Sbjct:   20 VTYSKPRLATFWHYARVELVPPTLGEIPTAIQSMKSIIHSAQTGNFKHLTVKEAVLNGLVATEVWMWFYIGEIIGKRGIVGYD 102
[Close]
BLAST of ATP synthase subunit vs. TrEMBL
Match: A0A074ZC94 (ATP synthase subunit OS=Opisthorchis viverrini OX=6198 GN=T265_07495 PE=3 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 3.078e-26
Identity = 46/96 (47.92%), Postives = 65/96 (67.71%), Query Frame = 2 
Query:   83 QKLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYSRIPGCY 370
             K++   +   +P L KF++YA+VEMRPPT SDI  A     +L+ +  + +W  + +K+ ++N VVT EV+ WFF GEIIGRRS  GYSR+PGCY
Sbjct:   13 SKVASTGISKGRPALSKFMNYARVEMRPPTLSDIGPAVAEATQLINAAKSGRWKEVTVKDGLLNAVVTIEVLAWFFIGEIIGRRSILGYSRVPGCY 108
[Close]
BLAST of ATP synthase subunit vs. TrEMBL
Match: A0A4S2LY81 (ATP synthase subunit OS=Opisthorchis felineus OX=147828 GN=CRM22_005362 PE=3 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 7.188e-26
Identity = 45/96 (46.88%), Postives = 65/96 (67.71%), Query Frame = 2 
Query:   83 QKLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYSRIPGCY 370
             K++   +   +P L KF++YA+VEMRPPT SDI  A     +L+ +  + +W  + +K+ ++N VVT EV+ WFF GEIIGRRS  GYS++PGCY
Sbjct:   13 SKVASTGISKGRPALSKFMTYARVEMRPPTLSDIGPAVAEATQLISAAKSGRWKEVTVKDGVLNAVVTIEVLAWFFIGEIIGRRSILGYSKVPGCY 108
[Close]
BLAST of ATP synthase subunit vs. TrEMBL
Match: A0A504YX86 (ATP synthase subunit g OS=Fasciola gigantica OX=46835 GN=FGIG_10225 PE=4 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 4.411e-25
Identity = 48/100 (48.00%), Postives = 64/100 (64.00%), Query Frame = 2 
Query:   71 VQKLQKLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYSRIPGCY 370
            V    K+  + L   KP + KFI+YAKVEMRPP+ +D+  A     +L+ +    KW ++  KE ++N VVT EV+ WFF GEIIGRRS  GYSR+PG Y
Sbjct:    5 VNASSKVISVLLTKGKPAISKFITYAKVEMRPPSVADLTPALAEANRLIAAAKAGKWKNVTTKEGLLNAVVTMEVLAWFFVGEIIGRRSIIGYSRVPGGY 104
[Close]
BLAST of ATP synthase subunit vs. TrEMBL
Match: A0A2H1BTK4 (ATP synthase subunit OS=Fasciola hepatica OX=6192 GN=D915_010253 PE=3 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 4.558e-25
Identity = 48/100 (48.00%), Postives = 64/100 (64.00%), Query Frame = 2 
Query:   71 VQKLQKLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYSRIPGCY 370
            V    K+  + L   KP + KFI+YAKVEMRPP+ +D+  A     +L+ +    KW ++  KE ++N VVT EV+ WFF GEIIGRRS  GYSR+PG Y
Sbjct:    5 VNASSKVISVLLTKGKPAISKFITYAKVEMRPPSMADLTPALAEANRLIAAAKAGKWKNVTTKEGLLNAVVTMEVLAWFFVGEIIGRRSIIGYSRVPGGY 104
[Close]
BLAST of ATP synthase subunit vs. TrEMBL
Match: A0A183RKK6 (ATP synthase subunit OS=Schistosoma rodhaini OX=6188 PE=3 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 2.389e-24
Identity = 47/95 (49.47%), Postives = 62/95 (65.26%), Query Frame = 2 
Query:   86 KLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYSRIPGCY 370
            K+S   +    PRL KF  YA+VE+RPPT +D+  A     KL+ S  +  W ++ +KE ++N VVT EV+ WFF GEIIGRRSF GYSR+P  Y
Sbjct:   14 KVSSFVIQETSPRLTKFREYARVELRPPTQADLKPAMEQATKLICSFKSGAWKNVSVKEGLVNAVVTVEVLCWFFIGEIIGRRSFLGYSRVPHTY 108
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BLAST of ATP synthase subunit vs. Ensembl Cavefish
Match: atp5l (ATP synthase membrane subunit g [Source:NCBI gene;Acc:103035071])

HSP 1 Score: 64.6994 bits (156), Expect = 5.774e-14
Identity = 30/80 (37.50%), Postives = 48/80 (60.00%), Query Frame = 2 
Query:   71 VQKLQKLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFF 310
            V K+  +    + Y+KPRL  F  YA+VE+ PPTP++IP A  G + L+K   T +     +++A  NG+V  +V+ WF+
Sbjct:    9 VAKVPTMVGAAVSYSKPRLATFWYYARVELVPPTPAEIPKAMAGFSNLIKGFQTGRLGQTTVRDAFRNGLVATDVLMWFY 88
[Close]
BLAST of ATP synthase subunit vs. Ensembl Nematostella
Match: EDO39978 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S8G1])

HSP 1 Score: 73.1738 bits (178), Expect = 1.334e-17
Identity = 36/96 (37.50%), Postives = 56/96 (58.33%), Query Frame = 2 
Query:   65 AYVQKLQKLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYS 352
            A   +L   +  T   A+P L KF + A+VE+ PP PS+ P        L  + ++ ++ ++ +KE + N +V AE+ FWF+ GEIIGRRS  GY+
Sbjct:   18 ALATRLTFQATATARKAQPMLGKFWTNARVELAPPMPSEWPAIQKSFMNLKDAALSGRFLNVTVKEGVANTLVAAEIAFWFYIGEIIGRRSLIGYN 113
[Close]
BLAST of ATP synthase subunit vs. Ensembl Medaka
Match: atp5l (ATP synthase membrane subunit g [Source:NCBI gene;Acc:101173244])

HSP 1 Score: 80.4925 bits (197), Expect = 3.470e-20
Identity = 39/94 (41.49%), Postives = 57/94 (60.64%), Query Frame = 2 
Query:   71 VQKLQKLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYS 352
            V K+  L    + Y+KPRL  F  YA+VE+ PP+P++IP A  G + L+K   T +     +K+A+ NG+V  EV+ WF+ GE IG+ S  GY 
Sbjct:    9 VAKVPSLVGAAVTYSKPRLATFWYYARVELVPPSPAEIPKAIEGASNLIKGFQTGRLGQTTVKDALRNGLVATEVLMWFYIGECIGKGSIIGYD 102
[Close]
BLAST of ATP synthase subunit vs. Planmine SMEST
Match: SMESG000021514.1 (SMESG000021514.1)

HSP 1 Score: 223.787 bits (569), Expect = 1.249e-76
Identity = 109/110 (99.09%), Postives = 110/110 (100.00%), Query Frame = 2 
Query:   62 MAYVQKLQKLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYSRIPGCYRVPGVFD 391
            MAYVQKLQKLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWAS+PMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYSRIPGCYRVPGVFD
Sbjct:    1 MAYVQKLQKLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAKLVKSTVTFKWASVPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYSRIPGCYRVPGVFD 110
The following BLAST results are available for this feature:
BLAST of ATP synthase subunit vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
ATP5MG1.964e-2446.81ATP synthase membrane subunit g [Source:HGNC Symbo... [more]
ATP5MGL1.398e-1842.39ATP synthase membrane subunit g like [Source:HGNC ... [more]
ATP5MG4.369e-842.19ATP synthase membrane subunit g [Source:HGNC Symbo... [more]
AP001267.51.048e-742.86novel protein[more]
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BLAST of ATP synthase subunit vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
asg-11.670e-1442.17Probable ATP synthase subunit g 1, mitochondrial ... [more]
asg-24.151e-1140.00Probable ATP synthase subunit g 2, mitochondrial ... [more]
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BLAST of ATP synthase subunit vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 4
Match NameE-valueIdentityDescription
ATPsynG8.604e-2548.19gene:FBgn0010612 transcript:FBtr0080167[more]
ATPsynG8.604e-2548.19gene:FBgn0010612 transcript:FBtr0110872[more]
ATPsynG8.604e-2548.19gene:FBgn0010612 transcript:FBtr0305994[more]
ATPsynGL8.950e-1438.30gene:FBgn0031941 transcript:FBtr0079481[more]
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BLAST of ATP synthase subunit vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
atp5l1.001e-2040.43ATP synthase, H+ transporting, mitochondrial F0 co... [more]
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BLAST of ATP synthase subunit vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
xkrx9.277e-1937.50XK related X-linked [Source:Xenbase;Acc:XB-GENE-95... [more]
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BLAST of ATP synthase subunit vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Atp5l5.039e-2340.59ATP synthase, H+ transporting, mitochondrial F0 co... [more]
Gm54268.976e-2238.61predicted pseudogene 5426 [Source:MGI Symbol;Acc:M... [more]
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BLAST of ATP synthase subunit vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q5RFH0|ATP5L_PONAB9.977e-2547.87ATP synthase subunit g, mitochondrial OS=Pongo abe... [more]
sp|O75964|ATP5L_HUMAN9.431e-2446.81ATP synthase subunit g, mitochondrial OS=Homo sapi... [more]
sp|Q9CPQ8|ATP5L_MOUSE3.524e-2240.59ATP synthase subunit g, mitochondrial OS=Mus muscu... [more]
sp|Q28852|ATP5L_BOVIN3.761e-2245.16ATP synthase subunit g, mitochondrial OS=Bos tauru... [more]
sp|Q6PDU7|ATP5L_RAT4.075e-1943.37ATP synthase subunit g, mitochondrial OS=Rattus no... [more]
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BLAST of ATP synthase subunit vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A074ZC943.078e-2647.92ATP synthase subunit OS=Opisthorchis viverrini OX=... [more]
A0A4S2LY817.188e-2646.88ATP synthase subunit OS=Opisthorchis felineus OX=1... [more]
A0A504YX864.411e-2548.00ATP synthase subunit g OS=Fasciola gigantica OX=46... [more]
A0A2H1BTK44.558e-2548.00ATP synthase subunit OS=Fasciola hepatica OX=6192 ... [more]
A0A183RKK62.389e-2449.47ATP synthase subunit OS=Schistosoma rodhaini OX=61... [more]
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BLAST of ATP synthase subunit vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
atp5l5.774e-1437.50ATP synthase membrane subunit g [Source:NCBI gene;... [more]
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BLAST of ATP synthase subunit vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ATP synthase subunit vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ATP synthase subunit vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO399781.334e-1737.50Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of ATP synthase subunit vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
atp5l3.470e-2041.49ATP synthase membrane subunit g [Source:NCBI gene;... [more]
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BLAST of ATP synthase subunit vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000021514.11.249e-7699.09SMESG000021514.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031423 ID=SMED30031423|Name=ATP synthase subunit|organism=Schmidtea mediterranea sexual|type=transcript|length=472bp
ATCAGCTAATTGCATAAAACTAATTTTGTTTTCTTGTTTAAAATCATTTA
GTAAATATTAAATGGCATATGTTCAAAAATTACAGAAATTAAGTCAAATT
ACTTTAGATTATGCAAAACCTCGATTAGATAAATTCATTTCATATGCGAA
AGTTGAAATGCGGCCTCCCACACCTAGTGATATACCTCTAGCAGCCATCG
GGTTGGCAAAACTTGTTAAATCTACTGTCACTTTTAAATGGGCATCCATG
CCAATGAAAGAAGCTATTATTAATGGAGTGGTAACCGCTGAAGTTGTATT
TTGGTTTTTTGCCGGTGAAATAATTGGTAGAAGATCATTTTTTGGATATA
GCAGAATACCTGGATGTTATCGTGTTCCGGGTGTTTTCGACTAGATCGAG
GAAAGGGATAGTTAAATATAAAATAGTTATTAAATAAAGTTTGAAATAAA
TACTATTGTCTTGAAAAAAAAA
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protein sequence of SMED30031423-orf-1

>SMED30031423-orf-1 ID=SMED30031423-orf-1|Name=SMED30031423-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=111bp
MAYVQKLQKLSQITLDYAKPRLDKFISYAKVEMRPPTPSDIPLAAIGLAK
LVKSTVTFKWASMPMKEAIINGVVTAEVVFWFFAGEIIGRRSFFGYSRIP
GCYRVPGVFD*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
PLANA:0000418head
PLANA:0000429neoblast
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR006808ATP_synth_F0_gsu_mt
Vocabulary: cellular component
TermDefinition
GO:0000276mitochondrial proton-transporting ATP synthase complex, coupling factor F(o)
GO:0005739mitochondrion
GO:0005743mitochondrial inner membrane
GO:0005753mitochondrial proton-transporting ATP synthase complex
GO:0016020membrane
GO:0045263proton-transporting ATP synthase complex, coupling factor F(o)
Vocabulary: biological process
TermDefinition
GO:0015986ATP synthesis coupled proton transport
GO:0006754ATP biosynthetic process
GO:0006810transport
GO:0006811ion transport
GO:0015992proton transport
Vocabulary: molecular function
TermDefinition
GO:0015078hydrogen ion transmembrane transporter activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006808ATP synthase, F0 complex, subunit G, mitochondrialPFAMPF04718ATP-synt_Gcoord: 6..96
e-value: 5.6E-21
score: 75.2
IPR006808ATP synthase, F0 complex, subunit G, mitochondrialPANTHERPTHR12386FAMILY NOT NAMEDcoord: 4..97
NoneNo IPR availablePANTHERPTHR12386:SF26ATP SYNTHASE SUBUNIT G 2, MITOCHONDRIAL-RELATEDcoord: 4..97