Protein amnionless

Overview
NameProtein amnionless
Smed IDSMED30031417
Length (bp)1497
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Protein amnionless (SMED30031417) t-SNE clustered cells

Violin plots show distribution of expression levels for Protein amnionless (SMED30031417) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Protein amnionless (SMED30031417) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Protein amnionless (SMED30031417) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30031417

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
protonephridiaSMED30031417SMESG000076397.1 dd_Smed_v4_5556_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30031417SMESG000076397.1 dd_Smed_v4_5556_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30031417SMESG000076397.1 dd_Smed_v4_5556_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031417SMESG000076397.1 Contig37755uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031417SMESG000076397.1 Contig37755newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031417SMESG000076397.1 Contig45102uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031417SMESG000076397.1 Contig45102newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Protein amnionless vs. Ensembl Human
Match: AMN (amnion associated transmembrane protein [Source:HGNC Symbol;Acc:HGNC:14604])

HSP 1 Score: 90.8929 bits (224), Expect = 6.495e-19
Identity = 64/255 (25.10%), Postives = 108/255 (42.35%), Query Frame = 1 
Query:   91 KLFIANTNFNNSKNWKEAQVPCSGNHLVFPK-RISRVFINAMSAPLQVMDIDFPINGEIIL--GNSLQITAFGNQVDDCDSTVSHFTKVAPDDWYNPLNWCQADDINWICLPKPQIEASLVPSASDFVVFPQHSTFYVGINHSEPRILAKSIFFRGTIYSRSYQFQQKIKKTFTRKFVEFVDLNSSYPPQIELQGNKCKKDYCIVNSNLQRMDAICKYV------TCQTPKCGHSVKPKGSCCDLCGH--LLTVG 822
            KL++ NT+F+ + NW + + PC+G  + FP  ++  V +    A   V D+  P++GE++L  G    ++  G+ +D      + F       W++P  W   D+   +      ++A  VP   D V FP  ++F VG+      +  +SI   G  ++R       +     R       L    P  + +    C      V  N +    IC  +       C    C  +++P+G CCDLCG   LLT G
Sbjct:   22 KLWVPNTDFDVAANWSQNRTPCAGGAVEFPADKMVSVLVQEGHA---VSDMLLPLDGELVLASGAGFGVSDVGSHLDCGAGEPAVFRDSDRFSWHDPHLWRSGDEAPGLFF----VDAERVPCRHDDVFFPPSASFRVGLGPGASPVRVRSISALGRTFTRDEDLAVFLASRAGR-------LRFHGPGALSVGPEDCADPSGCVCGNAEAQPWICAALLQPLGGRCPQAACHSALRPQGQCCDLCGAVVLLTHG 262
[Close]
BLAST of Protein amnionless vs. Ensembl Fly
Match: Amnionless (gene:FBgn0025686 transcript:FBtr0078134)

HSP 1 Score: 77.0258 bits (188), Expect = 1.019e-14
Identity = 66/328 (20.12%), Postives = 138/328 (42.07%), Query Frame = 1 
Query:   85 AVKLFIANTNFNNSKNWKEAQVPCSGNHLVFPKRISRVFINAMSAPLQVMDIDFPINGEIILGNSLQITAFGNQVDDC--DSTVSHFTKVAPDDWYNPLNWCQADDINWICLPKPQIEASLVPSASDFVVFPQHSTFYVGINHSEPRILAKSIFFRGTIYSRSYQFQQKIKKTFTRKFVEFVDLNSSYPPQIELQGNKCKKDYCIVNSNLQRM-DAICKYVT--CQTPKCGHSVKPKGSCCDLCGHLL---TVGLSKESNKTKIGDWLNKYLNSQFQKKIIFDLLSVEIREFQLTISPLITESEQFDKIILEIVENIKSRPTSEGVIL 1044
            A K +    +FN+   W +  +PC+ + +VFP+    +    +   + +  + FP  G I+L     IT   ++   C  D   ++        W++P  W   D         P++E   +P   + V+   H      + + +   L + +   G+  S++Y     +++  +R   +F+  N+ Y     +Q    + + C  + + +R+ + +C  V   C+TP C   V+P GSCC +CG +L   T   ++ S K  +    N       + +I   +  V  ++F   +  ++T+  ++ +  LE ++  +      G  L
Sbjct:   20 ATKWYGGGMDFNDPTAWLDDHLPCAQDLVVFPEYYPALL--PLPEDISIDGLVFPREGAILLAEESTITFGSDKQPSCESDENKAYLKPPKSSKWFDPGTW--TDKSKEFSTFTPELER--IPCDDEQVIIKGHGPLAFDLENVQYLRLGQ-LILAGSSISKTY-----LEQLISRDLGQFLFYNAEY-----VQVEYYRGELCGCHKDFERLLEPVCHNVQEQCETPHCLSPVRPLGSCCLICGAILSTPTTHCTEGSRKELVAQISNLISQESLKDQIGLHVEFVGSQKFGNCLQAVVTDRHKYSERSLEFLQQNEHNWNKTGTTL 330
[Close]
BLAST of Protein amnionless vs. Ensembl Fly
Match: Amnionless (gene:FBgn0025686 transcript:FBtr0330647)

HSP 1 Score: 77.0258 bits (188), Expect = 1.019e-14
Identity = 66/328 (20.12%), Postives = 138/328 (42.07%), Query Frame = 1 
Query:   85 AVKLFIANTNFNNSKNWKEAQVPCSGNHLVFPKRISRVFINAMSAPLQVMDIDFPINGEIILGNSLQITAFGNQVDDC--DSTVSHFTKVAPDDWYNPLNWCQADDINWICLPKPQIEASLVPSASDFVVFPQHSTFYVGINHSEPRILAKSIFFRGTIYSRSYQFQQKIKKTFTRKFVEFVDLNSSYPPQIELQGNKCKKDYCIVNSNLQRM-DAICKYVT--CQTPKCGHSVKPKGSCCDLCGHLL---TVGLSKESNKTKIGDWLNKYLNSQFQKKIIFDLLSVEIREFQLTISPLITESEQFDKIILEIVENIKSRPTSEGVIL 1044
            A K +    +FN+   W +  +PC+ + +VFP+    +    +   + +  + FP  G I+L     IT   ++   C  D   ++        W++P  W   D         P++E   +P   + V+   H      + + +   L + +   G+  S++Y     +++  +R   +F+  N+ Y     +Q    + + C  + + +R+ + +C  V   C+TP C   V+P GSCC +CG +L   T   ++ S K  +    N       + +I   +  V  ++F   +  ++T+  ++ +  LE ++  +      G  L
Sbjct:   20 ATKWYGGGMDFNDPTAWLDDHLPCAQDLVVFPEYYPALL--PLPEDISIDGLVFPREGAILLAEESTITFGSDKQPSCESDENKAYLKPPKSSKWFDPGTW--TDKSKEFSTFTPELER--IPCDDEQVIIKGHGPLAFDLENVQYLRLGQ-LILAGSSISKTY-----LEQLISRDLGQFLFYNAEY-----VQVEYYRGELCGCHKDFERLLEPVCHNVQEQCETPHCLSPVRPLGSCCLICGAILSTPTTHCTEGSRKELVAQISNLISQESLKDQIGLHVEFVGSQKFGNCLQAVVTDRHKYSERSLEFLQQNEHNWNKTGTTL 330
[Close]
BLAST of Protein amnionless vs. Ensembl Zebrafish
Match: amn (amnion associated transmembrane protein [Source:ZFIN;Acc:ZDB-GENE-060810-59])

HSP 1 Score: 85.8853 bits (211), Expect = 1.783e-17
Identity = 74/319 (23.20%), Postives = 134/319 (42.01%), Query Frame = 1 
Query:   91 KLFIANTNFNNSKNWKEAQVPCSGNHLVF-PKRISRVFINAMSAPLQVMDIDFPINGEIILGNSLQITAFGNQVDDCDSTV-SHFTKVAPDDWYNPLNWCQADDINWICLPKPQ--IEASLVPSASDFVVFPQHSTFYVGINHSEPRILAKSIFFRGTIYSRSYQFQQKIKKTFTRKFVEFVDLNSSYPPQIELQGNKCKKDYCIVNSNLQRMDAICKYVTCQTPKCGHSVKPKGSCCDLCGHLLTVGLSKESNKTKIGDWLNK-YLNSQFQKKIIFDLLSVEIREFQLTISPLITESEQFDKIILEIVENIKSRPTSE 1032
            K +I +TNF N+ NW +  VPC  + + F   R   V++        +  +  P++GE+IL +    T        C S V + F       W++P  W  A  I+ +     Q  +    VP  +D VVF   ++F V I+ S+  +  KS+   G  ++ S +F Q +  +  +       L       I +  + C +    +  N +  + IC  V C T +C   +   G CC++CG L+ V  S   N       L   +LN+   + +   +  V   +  L + P    +++   ++L+    ++S   +E
Sbjct:   27 KQWIPDTNFENATNWDKGSVPCGNDQVEFLANRKVSVYVETAHT---ITGMSLPVDGELILASGAGFTVREGGDPGCGSGVTAQFKDSESLKWFDPSLWVAATTIDDLHRGTFQFSVHEESVPCQNDDVVFRDATSFRVEIS-SDHDVPVKSVSVLGKKFTSSSEFSQYLSSSSGK-------LQFHGTATISISSSGCGEISGCICDNSRNREKICANVRCDTLECKKPLHALGHCCNVCGALVYVQFSSSFNFESYRQRLQHLFLNTDKYRSVQMAMSKVSREQRLLGVIPF-GATQEIQVLLLDQKTGLESGNLAE 333
[Close]
BLAST of Protein amnionless vs. Ensembl Xenopus
Match: AMN (amnion associated transmembrane protein [Source:NCBI gene;Acc:100485586])

HSP 1 Score: 92.0485 bits (227), Expect = 2.539e-19
Identity = 125/494 (25.30%), Postives = 207/494 (41.90%), Query Frame = 1 
Query:   76 SGNAV-KLFIANTNFNNSKNWKEAQVPCSGNHLVF--PKRISRVFINAMSAPLQVMDIDFPINGEIILGNSLQITAFGNQVDDCD-STVSHFTKVAPDDWYNPLNWCQADDINWICLPKP--QIEASLVPSASDFVVFPQHSTFYVGINHSEPRILAKSIFFRGTIYSRSYQFQQKIKKTFTRKFVEFVDLNSSYPPQIELQGNKCK-KDYCIVNSNLQRMDAICKYV------TCQTPKCGHSVKPKGSCCDLCGHLLTVGLSK----ESNKTKIGDW---LNKYLNSQFQKKIIFDLLSV-------EIREFQLTI------SPLITESEQFDKIILEIVENIKSRPTSEGVI---LVKCTSCKTPKAFSRDPQKSKYWISLLVIGVFMLFVIALLLYMNRKSGTSRFQFNFNLPKMPKFQFPRMN------FDNFRSNFPRVRLHETFDENSRG------HSEFVEAIELPDTGTEVSFDNPIYVAEIDDS 1413
            S NAV K +I NTN+ N+ NW E +VPCS + ++F   K++S V++ A  +   + D+  P +GE IL  S    A    + +C   +   F       W +P  W     ++ +   K    ++A  VP   D V+FP  ++F V I  ++  I  KSI   G  +SR   F Q  +++ T K      L  + P  +++   KC+ K  C   +NL  +  IC  +       C    C + ++P G CC++CG  +++  S     E+ + ++      L KY   +     +   L+V        I E Q+ I      S   ++++Q   +  +I+ +I+S   + G+I   L+  T   T    +  P  +   I   VIG  +   +    Y   ++G  R      LP      F RM         N  S      + +   +NS          E ++ I +  TG  V F NP+Y    DDS
Sbjct:    3 STNAVYKQWIPNTNYENALNWNEQRVPCSQDTVMFDSGKKVS-VYVQASHS---LKDMYLPWDGEFILSPSAGFLASTEDIAECGKGSTIMFRDTDQHKWLDPTLWLSGLSVDDLEGGKSLFHVDAERVPCQYDDVIFPPETSFCVSIEATDSAIQLKSISVMGQKFSRDEDFSQ-YRQSNTGK------LQFTGPGSLQITNTKCRDKTGCECGNNL-FLREICSNLLRHHGNKCPEVTCANPLQPVGHCCEICGATVSLEHSSQFDLENYRNRLTHTFLSLTKYSGVKLAISKVQKPLTVLGIIPRGSIPEIQIVIIDDKNGSQTGSDAQQ---LAYDIMSDIQSHGQTFGIIKGKLLLATGSGTSTHKTDIPVGT---IFASVIGFIICVALIGATYFLYRTGVIR------LPSFHAITFKRMRNRTEEVVTNMESKGFENPIFDGIPDNSTDVPPLYEGEEALKGIVIRQTG--VQFTNPLY----DDS 466
[Close]
BLAST of Protein amnionless vs. Ensembl Mouse
Match: Amn (amnionless [Source:MGI Symbol;Acc:MGI:1934943])

HSP 1 Score: 78.9518 bits (193), Expect = 3.042e-15
Identity = 72/327 (22.02%), Postives = 135/327 (41.28%), Query Frame = 1 
Query:   85 AVKLFIANTNFNNSKNWKEAQVPCSGNHLVFPK-RISRVFINAMSAPLQVMDIDFPINGEIILGNSLQITAFGNQVDDCDSTVSHFTKVAPD--DWYNPLNWCQADDINWICLPKPQIEASLVPSASDFVVFPQHSTFYVGINHSEPRILAKSIFFRGTIYSRSYQFQQKIKKTFTR-KFVEFVDLNSSYPPQIELQGNKCKKDYC---IVNSNLQRMDAICKYVTCQTPKCGHSVKPKGSCCDLCGHLLTVGLSKESNKTKIGDWLNKYLNSQFQKKIIFDLLSVEIREFQLTISPLITESEQFDKIILEIVENIKSRPTSEGVIL 1044
            A KL++ NT+F+ + NW + + PC+G+ + FP  ++  V +    A   + D+  P++GE++L +   ++A G   D   +  +      PD   W +P  W        +      ++A  VP + D V+FP+  +F V +      +  +S+   G  +SR       +     R +F     L        +  G  C        I  S LQ +   C    CQ P     + P+G CCDLCG ++++      +  +    L      Q Q + +   +S  +R+    I  ++ E+E       ++   +     ++G +L
Sbjct:   20 AYKLWVPNTSFDTASNWNQNRTPCAGDAVQFPADKMVSVLVRDSHA---ISDMLLPLDGELVLASGAALSAAGGDSDPACNPGAPLLFRNPDRFSWLDPHLWSSGTQAPGLF----SVDAERVPCSYDDVLFPRDGSFRVALGPGPNPVHVRSVSAVGQTFSRDEDLTAFLASREGRLRFHGSGALRVGSQACTDASGCVCGNAEMLPWICASLLQPLGGRCPQAACQDP-----LLPQGQCCDLCGAIVSLTHDPTFDLERYRARLLDLFLKQPQYQGLQVAVSKVLRDAHTEIQVVLVETEHATGAAGQLGHALLQDAVAQGSVL 334
[Close]
BLAST of Protein amnionless vs. UniProt/SwissProt
Match: sp|Q9BXJ7|AMNLS_HUMAN (Protein amnionless OS=Homo sapiens OX=9606 GN=AMN PE=1 SV=2)

HSP 1 Score: 90.8929 bits (224), Expect = 3.119e-18
Identity = 64/255 (25.10%), Postives = 108/255 (42.35%), Query Frame = 1 
Query:   91 KLFIANTNFNNSKNWKEAQVPCSGNHLVFPK-RISRVFINAMSAPLQVMDIDFPINGEIIL--GNSLQITAFGNQVDDCDSTVSHFTKVAPDDWYNPLNWCQADDINWICLPKPQIEASLVPSASDFVVFPQHSTFYVGINHSEPRILAKSIFFRGTIYSRSYQFQQKIKKTFTRKFVEFVDLNSSYPPQIELQGNKCKKDYCIVNSNLQRMDAICKYV------TCQTPKCGHSVKPKGSCCDLCGH--LLTVG 822
            KL++ NT+F+ + NW + + PC+G  + FP  ++  V +    A   V D+  P++GE++L  G    ++  G+ +D      + F       W++P  W   D+   +      ++A  VP   D V FP  ++F VG+      +  +SI   G  ++R       +     R       L    P  + +    C      V  N +    IC  +       C    C  +++P+G CCDLCG   LLT G
Sbjct:   22 KLWVPNTDFDVAANWSQNRTPCAGGAVEFPADKMVSVLVQEGHA---VSDMLLPLDGELVLASGAGFGVSDVGSHLDCGAGEPAVFRDSDRFSWHDPHLWRSGDEAPGLFF----VDAERVPCRHDDVFFPPSASFRVGLGPGASPVRVRSISALGRTFTRDEDLAVFLASRAGR-------LRFHGPGALSVGPEDCADPSGCVCGNAEAQPWICAALLQPLGGRCPQAACHSALRPQGQCCDLCGAVVLLTHG 262
[Close]
BLAST of Protein amnionless vs. UniProt/SwissProt
Match: sp|Q99JB7|AMNLS_MOUSE (Protein amnionless OS=Mus musculus OX=10090 GN=Amn PE=1 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 2.128e-14
Identity = 72/327 (22.02%), Postives = 135/327 (41.28%), Query Frame = 1 
Query:   85 AVKLFIANTNFNNSKNWKEAQVPCSGNHLVFPK-RISRVFINAMSAPLQVMDIDFPINGEIILGNSLQITAFGNQVDDCDSTVSHFTKVAPD--DWYNPLNWCQADDINWICLPKPQIEASLVPSASDFVVFPQHSTFYVGINHSEPRILAKSIFFRGTIYSRSYQFQQKIKKTFTR-KFVEFVDLNSSYPPQIELQGNKCKKDYC---IVNSNLQRMDAICKYVTCQTPKCGHSVKPKGSCCDLCGHLLTVGLSKESNKTKIGDWLNKYLNSQFQKKIIFDLLSVEIREFQLTISPLITESEQFDKIILEIVENIKSRPTSEGVIL 1044
            A KL++ NT+F+ + NW + + PC+G+ + FP  ++  V +    A   + D+  P++GE++L +   ++A G   D   +  +      PD   W +P  W        +      ++A  VP + D V+FP+  +F V +      +  +S+   G  +SR       +     R +F     L        +  G  C        I  S LQ +   C    CQ P     + P+G CCDLCG ++++      +  +    L      Q Q + +   +S  +R+    I  ++ E+E       ++   +     ++G +L
Sbjct:   20 AYKLWVPNTSFDTASNWNQNRTPCAGDAVQFPADKMVSVLVRDSHA---ISDMLLPLDGELVLASGAALSAAGGDSDPACNPGAPLLFRNPDRFSWLDPHLWSSGTQAPGLF----SVDAERVPCSYDDVLFPRDGSFRVALGPGPNPVHVRSVSAVGQTFSRDEDLTAFLASREGRLRFHGSGALRVGSQACTDASGCVCGNAEMLPWICASLLQPLGGRCPQAACQDP-----LLPQGQCCDLCGAIVSLTHDPTFDLERYRARLLDLFLKQPQYQGLQVAVSKVLRDAHTEIQVVLVETEHATGAAGQLGHALLQDAVAQGSVL 334
[Close]
BLAST of Protein amnionless vs. UniProt/SwissProt
Match: sp|Q6UKI2|AMNLS_CANLF (Protein amnionless OS=Canis lupus familiaris OX=9615 GN=AMN PE=1 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 7.172e-14
Identity = 62/256 (24.22%), Postives = 104/256 (40.62%), Query Frame = 1 
Query:   85 AVKLFIANTNFNNSKNWKEAQVPCSGNHLVFP--KRISRVFINAMSAPLQVMDIDFPINGEIILGNSLQITAFGNQVD-DCDSTVSHFTKVAPD--DWYNPLNWCQADDINWICLPKPQIEASLVPSASDFVVFPQHSTFYVGINHSEPRILAKSIFFRGTIYSRSYQFQQKIKKTFTRKFVEFVDLNSSYPPQIELQGNKCKKDYCIVNSNLQRMDAICKYV------TCQTPKCGHSVKPKGSCCDLCGHLLTV 819
            A KL++  T+F  + NW + + PC+G  + FP  K +S V    + A     D+  P +GE +L +     A     D DC +       + PD   W++P  W   D    +      ++A  VP   D VVFP  ++F VG+         +S+   G  ++R       +     R       L    P  + +    C      V  + +    IC  +       C    C  +++P+G CCDLCG ++++
Sbjct:   20 AYKLWVPTTDFEAAANWSQNRTPCAGAVVQFPADKAVSVV----VRASHGFSDMLLPRDGEFVLASGAGFGAADAGRDPDCGAGAPALF-LDPDRFSWHDPRLWRSGDAARGLF----SVDAERVPCRHDDVVFPPDASFRVGLGPGARPARVRSVQVLGQTFTRDEDLAAFLASRAGR-------LRFHGPGALRVGPGACADPSGCVCGDAEVQPWICAALLQPLGGRCPPAACPDALRPEGQCCDLCGAIVSL 259
[Close]
BLAST of Protein amnionless vs. UniProt/SwissProt
Match: sp|Q9VPN2|AMNLS_DROME (Protein amnionless OS=Drosophila melanogaster OX=7227 GN=Amnionless PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.021e-13
Identity = 66/328 (20.12%), Postives = 138/328 (42.07%), Query Frame = 1 
Query:   85 AVKLFIANTNFNNSKNWKEAQVPCSGNHLVFPKRISRVFINAMSAPLQVMDIDFPINGEIILGNSLQITAFGNQVDDC--DSTVSHFTKVAPDDWYNPLNWCQADDINWICLPKPQIEASLVPSASDFVVFPQHSTFYVGINHSEPRILAKSIFFRGTIYSRSYQFQQKIKKTFTRKFVEFVDLNSSYPPQIELQGNKCKKDYCIVNSNLQRM-DAICKYVT--CQTPKCGHSVKPKGSCCDLCGHLL---TVGLSKESNKTKIGDWLNKYLNSQFQKKIIFDLLSVEIREFQLTISPLITESEQFDKIILEIVENIKSRPTSEGVIL 1044
            A K +    +FN+   W +  +PC+ + +VFP+    +    +   + +  + FP  G I+L     IT   ++   C  D   ++        W++P  W   D         P++E   +P   + V+   H      + + +   L + +   G+  S++Y     +++  +R   +F+  N+ Y     +Q    + + C  + + +R+ + +C  V   C+TP C   V+P GSCC +CG +L   T   ++ S K  +    N       + +I   +  V  ++F   +  ++T+  ++ +  LE ++  +      G  L
Sbjct:   20 ATKWYGGGMDFNDPTAWLDDHLPCAQDLVVFPEYYPALL--PLPEDISIDGLVFPREGAILLAEESTITFGSDKQPSCESDENKAYLKPPKSSKWFDPGTW--TDKSKEFSTFTPELER--IPCDDEQVIIKGHGPLAFDLENVQYLRLGQ-LILAGSSISKTY-----LEQLISRDLGQFLFYNAEY-----VQVEYYRGELCGCHKDFERLLEPVCHNVQEQCETPHCLSPVRPLGSCCLICGAILSTPTTHCTEGSRKELVAQISNLISQESLKDQIGLHVEFVGSQKFGNCLQAVVTDRHKYSERSLEFLQQNEHNWNKTGTTL 330
[Close]
BLAST of Protein amnionless vs. TrEMBL
Match: C3Z1J9 (Uncharacterized protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_82292 PE=4 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 1.557e-24
Identity = 89/339 (26.25%), Postives = 151/339 (44.54%), Query Frame = 1 
Query:   82 NAVKLFIANTNFNNSKNWKEAQVPCSGNHLVFPKRIS--RVFINAMSAPLQVMDIDFPINGEIILGNSLQITAFGNQVDD--CDSTVSHFTKVAPDDWYNPLNWCQADDINWICLPKPQ-----------------IEASLVPSASDFVVFPQHSTFYVGINHSEPRILAKSIFFRGTIYSRSYQFQQKIKKTFTRKFVEFVDLNSSYPP--QIELQGNKCKKDYCIVNSNLQRMDAICKYV--TCQTPKCGHSVKPKGSCCDLCGHLLTVGLSKESNKTKIGDWLNKYLNSQFQKKIIFDLLSVEIREFQLTISPLITE-SEQFDKIILEIVENIKSR 1020
             A K+++ NTN+ NS NW     PCS   + F ++ +   VF+      + V ++  P+NGEI+  + L++  FG+  DD  C +  + F    P DW NP NW   DD N +    P+                 + A +VP   D VVFP+++T+ V ++HS+     K + F G +Y R  +F Q +K        E  ++     P   I + G  CK+       N + MD IC      C    C   + P+GSCC +CG +L +            ++ + +    FQ +++ D +  ++RE+   +   + E + +    + E    +K R
Sbjct:   24 GAKKIWLKNTNWMNSANWDRGSPPCSKQVVSFQEKPNPYAVFV---QNDVSVGELVLPLNGEIVFADDLKVN-FGSFTDDPACMAGDNRFVGGEPGDWLNPHNWRVEDDGNLVEPMSPEDDSSPTSPGDDTDGFPVLHAEVVPCTHDTVVFPENATYLVRLDHSK---QVKHVDFTGKVYKRKPEFHQFLKS-------ENGEMQFKLGPSGDIAVMGQDCKELSGCYCGNEEHMDLICDLYGDNCPPLTCSSPITPRGSCCPMCGSVLLL------------NYDDTFDIMAFQDRLVVDFVP-KVREYTGRVKDRVREYTGRVKDRVREYTGRVKGR 335
[Close]
BLAST of Protein amnionless vs. TrEMBL
Match: A0A3S1BTZ2 (Uncharacterized protein OS=Elysia chlorotica OX=188477 GN=EGW08_000986 PE=4 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 2.545e-22
Identity = 114/451 (25.28%), Postives = 183/451 (40.58%), Query Frame = 1 
Query:   91 KLFIANTNFNNSKNWKEAQVPCSGNHLVFPKRISRVFINAMSAPLQVMDIDFPINGEIILGNSLQITAFGNQVDDCDSTVS--HFTKVAPDDWYNPLNWCQADDINWICLPKPQIEASLVPSASDFVVFPQHSTFYVGINHSEPRILAKSIFFRGTIYSRSYQFQQKIKKTFTRKFVEFVDLNSSYPPQIELQGNKCK-KDYCIVNSNLQRM-DAICKYV--TCQTPKCGHSVKPKGSCCDLCGHLLTVGLSKESNKTKIGDWLNKYLNSQFQKKIIFDLLSVEIREFQLTISPLITESEQFDKIILEIVENIKSRPTSEG-------VILVKCTSCKTPKAFSRDPQKSKYWISLLVIGVFM-LFVIALL--LYMNRKSGTSRFQFNFNLPKMPKFQ-FPRMNFDNFRSNFPRVRLHETFDENSRGHSEFVEAIELPDTGTEVSFDNPIY 1392
            K ++ NT+F N +NW   + PC GN +V     S V    ++  ++  ++  P +GE+ILG +  +       DDC        FT   P  W N  NWC A      C    +++   VP   D VVFP++  ++V ++ + P +  KS+   G+ +S    F   + +T  ++    V  N      +E+    C  K  C   +++  +   IC +V  +C  P+C  S+ P G CC +CG    +      +       LNK    Q QK +   +  +     QL ++   +  E   K+   I E+     T+ G         L   T   +P         S   I+ +V GV + L VIAL+  ++  R++G        NLP+MP     P +N        P +R         RG S         D G    F NP+Y
Sbjct:   22 KRWLPNTDFGNPQNWNAKRTPC-GNDIVIIGDDSPVVYVQINTTMK--ELVLPSSGELILGRNATLAFTSRSSDDCPDFGGDIEFTATEPKSWLNAKNWCSATTERGACDTAARLDVDKVPCKYDDVVFPRYHGYFVDLSQNVPDMTVKSLKISGSTFSSKKSFDNFLNQTDGKRMFR-VGKNGH---TLEVLRKVCNVKGGCACGNDIPELTKKICSFVEGSCNRPRCAKSIMPPGMCCRMCGAYFNITKGYGFDLDNFKSSLNKEF-FQKQKDVTSIVAVMTGNWLQLVLTD--SNGENSSKMAQSIKEDFNKDFTAGGHKYAIDHYQLFLATIEPSPHNRKSSSSLSGGSIAGIVFGVLIFLIVIALIVGVFTFRRTGRPNLP---NLPRMPALHSVPGLN------RIPGLRGRRA---TPRGDSGTPRTAAHVDPG----FANPMY 446
[Close]
BLAST of Protein amnionless vs. TrEMBL
Match: A0A3Q0QSU7 (Amnion associated transmembrane protein OS=Amphilophus citrinellus OX=61819 PE=4 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 4.069e-21
Identity = 98/414 (23.67%), Postives = 170/414 (41.06%), Query Frame = 1 
Query:   91 KLFIANTNFNNSKNWKEAQVPCSGNHLVFP-KRISRVFINAMSAPLQVMDIDFPINGEIILGNSLQITAFGNQVDDCDSTVS-HFTKVAPDDWYNPLNWCQADDINWICLPKP--QIEASLVPSASDFVVFPQHSTFYVGINHSEPRILAKSIFFRGTIYSRSYQFQQKIKKTFTRKFVEFVDLNSSYPPQIELQGNKCKKDYCIVNSNLQRMDAICKYVTCQTPKCGHSVKPKGSCCDLCGHLLTVGLSKESNKTKIGDWLNKYLNSQFQKKIIFDLLSVEIREFQLTISPLITESEQFDKIILEIVENIKSRPTSEGVI--------LVKCTSCKTPKAFSRDPQKSKYWISLLVIGVFMLFVIALLLYMNRKSGT------SRFQFNFNLPKM----------PKFQFPRM 1248
            K +I +TN+ N  NW +  VPC  + + FP +R   VF+    A   V ++  P++GE IL +       G Q  DC + V+  F       W+NP  W  A  +N +        +    VP   D VVF   S+F V  + S   I  KS+   G  ++   +F Q +  +  R     +  + S    +   G  C+        N      IC  +TC +P C   ++P G CCD+CG ++T+   +  N     + +     +  Q K I   +S  ++  +L     +  S +   ++L+  + I+S   ++ ++        ++  T  +   A       S   +   V GVF+L  + ++L +  + G       SRF+ N ++P +          P F  P M
Sbjct:   22 KQWIPDTNYENKTNWDKGNVPCGNDIIQFPAQRKVSVFVETTHA---VQEMRMPLDGEFILTSGAGFYVVGGQDPDCGTGVTTEFKDSESLQWFNPTLWQTAATLNDLQQGNFLFSVHDESVPCHYDDVVFKALSSFRVDTSSSHSSIAVKSVSVLGNTFTSQSEFSQYLSSSSGR-----LQFHGSSAATVGNSG--CEDPSGCDCGNSVHHQQICSTITCDSPSCKKPLRPIGHCCDVCGAIVTIHFXEGFNLQTYRERIRHLFLALPQYKSIKLGMSKVLKSQRLMGIISLGTSSEIQVVMLDGEKGIQSAALAQDIVKDAHSHGSILGITGAEIQSASEEQSGGSAGLVVGFVFGVFLLITLIIILAVLIRKGVVQMPTLSRFKNNSDMPDLGGPLDYGFDNPMFDKPSM 425
[Close]
BLAST of Protein amnionless vs. TrEMBL
Match: A0A210PQB1 (Protein amnionless OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT10801 PE=4 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 7.342e-21
Identity = 99/458 (21.62%), Postives = 202/458 (44.10%), Query Frame = 1 
Query:   91 KLFIANTNFNNSKNWKEAQVPCSGNHLVFPKRISRVFINAMSAPLQVMDIDFPINGEIILGNSLQITAFGNQVDDCDSTVSHFTKVAPD-----DWYNPLNWCQADDINWICLPKPQIEASLVPSASDFVVFPQHSTFYVGINHSEPRILAKSIFFRGTIYSRSYQFQQKIKKTFTRKFVEFVDLNSSYPPQIELQGNKCKKDYCIV--NSNLQRMDAICKYVT--CQTPKCGHSVKPKGSCCDLCGHLLTVGLSKESNKTKIGDWLNKYL--NSQFQKKIIFDLLSVEIREFQLTISPLITESEQFDKIILEIVENIKSRPTSEGVILVKCTSCKTPKAFSRDPQK----------SKYWISLLVIGVFMLFVIALLLYMNRKSGTSRFQFNFNLPKMPKFQ-FPRMNFDNFRSNFPRVRLH--ETFDENSRGHSEFVEAIELPDTGTEVSFDNPIY 1392
            K+++++ NF N  NW + + PC  + +V  +    +++   +   Q++    P NGE+ILG+   + AF N+ D     +S  +++  +     DW +  NWC +D     C     +++   P  +D VVFP+ S+++V +  S   +   ++   G+ Y+ +  F Q +  T  ++    +  ++     +++   +C         N  L  +  ICKY +  C   +C   ++P GSCC++CG +L+V          + D L +    ++   K + + +  +     Q+ +S +    +Q  +   E+  ++ +         ++  S +T +    +P            S+  I+ +V+G+  + +I+L L      GT  ++     P+   F+ F R+   +F+ +FP+ RL    +F     G S   E +     G    FDNP+Y
Sbjct:   25 KVWMSDANFGNPSNWDKGRQPCGDDIVVIHEESPAIYVQVNTTLQQII---LPKNGEMILGSGSTL-AFTNEPDHSAGCLSSGSEIVYNLTYALDWLDYANWCPSDTETGSCKSLALLDSEQTPCKTDDVVFPKMSSYFVNL-ESGLSLSVNTLKITGSAYTTT-GFSQFLNTTDGKRMFP-ISPSTGSRSSVQIARRRCTDPTGCACGNDGLDILRRICKYRSPYCTRIRCKQPIRPSGSCCEICGAVLSVQYGTGFKFADLRDGLQRSFLDDNDNYKDVQYIVSKMSDGHVQIVLSDV--SGDQSVQAGREMKADLDNDNAGGFKYAIRSVSMETSRGSGLNPTHGPQTGRGLAMSRGEIAGIVVGLVTVLLISLFL------GTLFYRRKTRGPEDISFKMFDRI---SFKPSFPKPRLEVPPSFSFRFGGSSSVPETVGARSQG----FDNPMY 460
[Close]
BLAST of Protein amnionless vs. TrEMBL
Match: H2LTV5 (Amnion associated transmembrane protein OS=Oryzias latipes OX=8090 GN=amn PE=4 SV=2)

HSP 1 Score: 105.145 bits (261), Expect = 1.636e-20
Identity = 107/428 (25.00%), Postives = 179/428 (41.82%), Query Frame = 1 
Query:   91 KLFIANTNFNNSKNWKEAQVPCSGNHLVF-PKRISRVFINAMSAPLQVMDIDFPINGEIILGNSLQITAFGNQVDDCDS-TVSHFTKVAPDDWYNPLNWCQADDINWICLPKP--QIEASLVPSASDFVVFPQHSTFYVGINHSEPRILAKSIFFRGTIYSRSYQFQQKIK-KTFTRKFVEFVDLNSSYPPQIELQGNKCKKDYCIVNSNLQRMDAICKYVTCQTPKCGHSVKPKGSCCDLCGHLLTV----GLSKESNKTKIGDW---LNKYLNSQFQKKIIFD---LLSV----EIREFQLTISPLITESEQFDKIILEIVENIKSRPTSEGV-ILVKCTSCKTPKAFSRDPQKSKY-WISLLVIGVFMLFVIALLLYMNRKSGTSRFQFNFNLPKMPKFQFPRMNFDNFRSNFPRVRLHETFDEN 1311
            K +I +TN+ N  NW + +VPC  +  +F  +R   VF+    A   V ++  P++GE+IL +       G +   C S T + F       W+NP  W  A   N + L      +    VP   D VVF   S+F V  + S+P I  KS+   G  +    +F Q +  ++   +F     + +  P   +  G  C      VN  L     IC  V C T  C   + P G CC++CG ++T+    G + ++ + +I      L KY + Q     +F    LL V     I E Q+    +I + E   K+  ++ +++ +   S G  I +  +  +     + D   S    ++ +VI V ++  +  +  +  + G  R      LP MP    P ++  +FR N     L  T D  
Sbjct:   21 KQWIPDTNYENKTNWDKGKVPCGNDIALFSAQRKVSVFVETTHA---VQEMRLPVDGELILNSGAGFYVLGGKDPACGSGTTTEFQDSESLQWFNPALWQAAASQNDLQLGNFLFAVHEESVPCQQDDVVFKALSSFRVDTSSSQPTIPVKSVSVLGKTFDSRAEFSQYLSSRSGQMQFHGSSAVTAGNPGCGDSSGCDCGNS---VNHQL-----ICSTVKCATVSCKKPLSPVGHCCEVCGAIVTIDYTAGFTVQTYRERIHHLFLILPKYKSIQLGISKVFKSQRLLGVIPFSTIPEIQV----VIVDGED-GKVSEDLAQDLMTDIGSHGSEIGITGSEFQASSGNTSDLSGSNAGMVAGVVIAVLLMIALIAVGAVLIQKGVVR------LPSMPTITMPSLS--SFRRNTDVGDLGGTIDHG 424
[Close]
BLAST of Protein amnionless vs. Ensembl Cavefish
Match: amn (amnion associated transmembrane protein [Source:NCBI gene;Acc:103027369])

HSP 1 Score: 84.3445 bits (207), Expect = 4.424e-17
Identity = 62/263 (23.57%), Postives = 113/263 (42.97%), Query Frame = 1 
Query:   79 GNAVKLFIANTNFNNSKNWKEAQVPCSGNHLVFPKRIS-RVFINAMSAPLQVMDIDFPINGEIILGNSLQITAFGNQVDDCDS-TVSHFTKVAPDDWYNPLNWCQADDIN------WICLPKPQIEASLVPSASDFVVFPQHSTFYVGINHSEPRILAKSIFFRGTIYSRSYQFQQKIKKTFTRKFVEFVDLNSSYPPQIELQGNKCKKDYCIVNSNLQRMDAICKYVTC-QTPKCGHSVKPKGSCCDLCGHLLTVGLSKESN 840
            G   K +I +TNF N+ NW +  VPC  +  VFP +    V++  +     +  +  P++GE +L      +    Q   C +   + F  V    W++P  W  A  ++      ++      +    VP   D V+F   S+F V ++ +   +  KS+   G  +  + +F Q +  +  +  ++F   +S     I+++G+ C         N    + IC  V C Q   C   ++P G CCD+CG ++ +  S   N
Sbjct:   19 GGVYKQWIPDTNFENATNWDKGSVPCGSDQAVFPAQGKVAVYVGLVHT---LAGLSLPVDGEFVLAPGAGFSIRVGQDPGCGAGGTTQFKDVESLKWFDPDLWSAASSVDDFQKGSYLFF----VHEESVPCQYDDVLFRDASSFRVDVSGNTNTVPVKSVKVLGQRFESNSEFSQYLTSSLGK--LQFHGSSS-----IKVEGSSCTDVTGCECGNSANHERICANVACSQQLDCKKPLRPVGHCCDVCGTIMDIQFSGSFN 267
[Close]
BLAST of Protein amnionless vs. Ensembl Medaka
Match: amn (amnion associated transmembrane protein [Source:NCBI gene;Acc:101172082])

HSP 1 Score: 105.145 bits (261), Expect = 6.157e-24
Identity = 107/428 (25.00%), Postives = 179/428 (41.82%), Query Frame = 1 
Query:   91 KLFIANTNFNNSKNWKEAQVPCSGNHLVF-PKRISRVFINAMSAPLQVMDIDFPINGEIILGNSLQITAFGNQVDDCDS-TVSHFTKVAPDDWYNPLNWCQADDINWICLPKP--QIEASLVPSASDFVVFPQHSTFYVGINHSEPRILAKSIFFRGTIYSRSYQFQQKIK-KTFTRKFVEFVDLNSSYPPQIELQGNKCKKDYCIVNSNLQRMDAICKYVTCQTPKCGHSVKPKGSCCDLCGHLLTV----GLSKESNKTKIGDW---LNKYLNSQFQKKIIFD---LLSV----EIREFQLTISPLITESEQFDKIILEIVENIKSRPTSEGV-ILVKCTSCKTPKAFSRDPQKSKY-WISLLVIGVFMLFVIALLLYMNRKSGTSRFQFNFNLPKMPKFQFPRMNFDNFRSNFPRVRLHETFDEN 1311
            K +I +TN+ N  NW + +VPC  +  +F  +R   VF+    A   V ++  P++GE+IL +       G +   C S T + F       W+NP  W  A   N + L      +    VP   D VVF   S+F V  + S+P I  KS+   G  +    +F Q +  ++   +F     + +  P   +  G  C      VN  L     IC  V C T  C   + P G CC++CG ++T+    G + ++ + +I      L KY + Q     +F    LL V     I E Q+    +I + E   K+  ++ +++ +   S G  I +  +  +     + D   S    ++ +VI V ++  +  +  +  + G  R      LP MP    P ++  +FR N     L  T D  
Sbjct:   21 KQWIPDTNYENKTNWDKGKVPCGNDIALFSAQRKVSVFVETTHA---VQEMRLPVDGELILNSGAGFYVLGGKDPACGSGTTTEFQDSESLQWFNPALWQAAASQNDLQLGNFLFAVHEESVPCQQDDVVFKALSSFRVDTSSSQPTIPVKSVSVLGKTFDSRAEFSQYLSSRSGQMQFHGSSAVTAGNPGCGDSSGCDCGNS---VNHQL-----ICSTVKCATVSCKKPLSPVGHCCEVCGAIVTIDYTAGFTVQTYRERIHHLFLILPKYKSIQLGISKVFKSQRLLGVIPFSTIPEIQV----VIVDGED-GKVSEDLAQDLMTDIGSHGSEIGITGSEFQASSGNTSDLSGSNAGMVAGVVIAVLLMIALIAVGAVLIQKGVVR------LPSMPTITMPSLS--SFRRNTDVGDLGGTIDHG 424
[Close]
BLAST of Protein amnionless vs. Planmine SMEST
Match: SMESG000076397.1 (SMESG000076397.1)

HSP 1 Score: 924.465 bits (2388), Expect = 0.000e+0
Identity = 447/450 (99.33%), Postives = 448/450 (99.56%), Query Frame = 1 
Query:   70 FVSGNAVKLFIANTNFNNSKNWKEAQVPCSGNHLVFPKRISRVFINAMSAPLQVMDIDFPINGEIILGNSLQITAFGNQVDDCDSTVSHFTKVAPDDWYNPLNWCQADDINWICLPKPQIEASLVPSASDFVVFPQHSTFYVGINHSEPRILAKSIFFRGTIYSRSYQFQQKIKKTFTRKFVEFVDLNSSYPPQIELQGNKCKKDYCIVNSNLQRMDAICKYVTCQTPKCGHSVKPKGSCCDLCGHLLTVGLSKESNKTKIGDWLNKYLNSQFQKKIIFDLLSVEIREFQLTISPLITESEQFDKIILEIVENIKSRPTSEGVILVKCTSCKTPKAFSRDPQKSKYWISLLVIGVFMLFVIALLLYMNRKSGTSRFQFNFNLPKMPKFQFPRMNFDNFRSNFPRVRLHETFDENSRGHSEFVEAIELPDTGTEVSFDNPIYVAEIDDSKQ 1419
            FVSGNAVKLFIANTNFNNSKNWKEAQVPCSGNHLVFPKRI RVFINAMSAPLQVMDIDFPINGEIILGNSLQITAFGNQVDDCDSTVSHFTKVAPDDWYNPLNWCQADDINWICLPKPQIEASLVPSASDFVVFPQHSTFYVGINHSEPRILAKSIFFRGTIYSRSYQFQQKIKKTFTRKFVEFVDLNSSYPPQIELQGNKCKKDYCIVNSNLQRMDAICKYVTCQTPKCGHSVKPKGSCCDLCGHLLTVGLSKESNKTKIGDWLNKYLNSQFQKKIIFDLL+VEIREFQLTISPLITESEQFDKIILEIVENIKSRP SEGVILVKCTSCKTPKAFSRDPQKSKYWISLLVIGVFMLFVIALLLYMNRKSGTSRFQFNFNLPKMPKFQFPRMNFDNFRSNFPRVRLHETFDENSRGHSEFVEAIELPDTGTEVSFDNPIYVAEIDDSKQ
Sbjct:   11 FVSGNAVKLFIANTNFNNSKNWKEAQVPCSGNHLVFPKRIPRVFINAMSAPLQVMDIDFPINGEIILGNSLQITAFGNQVDDCDSTVSHFTKVAPDDWYNPLNWCQADDINWICLPKPQIEASLVPSASDFVVFPQHSTFYVGINHSEPRILAKSIFFRGTIYSRSYQFQQKIKKTFTRKFVEFVDLNSSYPPQIELQGNKCKKDYCIVNSNLQRMDAICKYVTCQTPKCGHSVKPKGSCCDLCGHLLTVGLSKESNKTKIGDWLNKYLNSQFQKKIIFDLLAVEIREFQLTISPLITESEQFDKIILEIVENIKSRPKSEGVILVKCTSCKTPKAFSRDPQKSKYWISLLVIGVFMLFVIALLLYMNRKSGTSRFQFNFNLPKMPKFQFPRMNFDNFRSNFPRVRLHETFDENSRGHSEFVEAIELPDTGTEVSFDNPIYVAEIDDSKQ 460
The following BLAST results are available for this feature:
BLAST of Protein amnionless vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
AMN6.495e-1925.10amnion associated transmembrane protein [Source:HG... [more]
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BLAST of Protein amnionless vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein amnionless vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Amnionless1.019e-1420.12gene:FBgn0025686 transcript:FBtr0078134[more]
Amnionless1.019e-1420.12gene:FBgn0025686 transcript:FBtr0330647[more]
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BLAST of Protein amnionless vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
amn1.783e-1723.20amnion associated transmembrane protein [Source:ZF... [more]
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BLAST of Protein amnionless vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
AMN2.539e-1925.30amnion associated transmembrane protein [Source:NC... [more]
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BLAST of Protein amnionless vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Amn3.042e-1522.02amnionless [Source:MGI Symbol;Acc:MGI:1934943][more]
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BLAST of Protein amnionless vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q9BXJ7|AMNLS_HUMAN3.119e-1825.10Protein amnionless OS=Homo sapiens OX=9606 GN=AMN ... [more]
sp|Q99JB7|AMNLS_MOUSE2.128e-1422.02Protein amnionless OS=Mus musculus OX=10090 GN=Amn... [more]
sp|Q6UKI2|AMNLS_CANLF7.172e-1424.22Protein amnionless OS=Canis lupus familiaris OX=96... [more]
sp|Q9VPN2|AMNLS_DROME1.021e-1320.12Protein amnionless OS=Drosophila melanogaster OX=7... [more]
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BLAST of Protein amnionless vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
C3Z1J91.557e-2426.25Uncharacterized protein OS=Branchiostoma floridae ... [more]
A0A3S1BTZ22.545e-2225.28Uncharacterized protein OS=Elysia chlorotica OX=18... [more]
A0A3Q0QSU74.069e-2123.67Amnion associated transmembrane protein OS=Amphilo... [more]
A0A210PQB17.342e-2121.62Protein amnionless OS=Mizuhopecten yessoensis OX=6... [more]
H2LTV51.636e-2025.00Amnion associated transmembrane protein OS=Oryzias... [more]
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BLAST of Protein amnionless vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
amn4.424e-1723.57amnion associated transmembrane protein [Source:NC... [more]
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BLAST of Protein amnionless vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein amnionless vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein amnionless vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein amnionless vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
amn6.157e-2425.00amnion associated transmembrane protein [Source:NC... [more]
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BLAST of Protein amnionless vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000076397.10.000e+099.33SMESG000076397.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031417 ID=SMED30031417|Name=Protein amnionless|organism=Schmidtea mediterranea sexual|type=transcript|length=1497bp
ATGAACTAGCAGTTAATTAATATTTAAATAATTAATAAAATGCTGAATTT
ATTGATATTAATATGCCTCTTTGTTTCTGGCAATGCTGTGAAGCTGTTCA
TTGCCAATACGAATTTTAACAACAGCAAGAATTGGAAGGAGGCCCAAGTG
CCATGTTCAGGGAATCATTTAGTGTTCCCCAAACGGATTTCGAGAGTGTT
CATCAATGCAATGTCGGCTCCGCTCCAGGTGATGGACATTGATTTCCCGA
TCAACGGCGAGATTATCCTCGGCAATTCTCTGCAGATCACCGCATTCGGC
AATCAGGTGGATGACTGCGATTCAACTGTGTCGCATTTCACCAAGGTGGC
GCCTGATGATTGGTACAATCCGCTCAATTGGTGCCAGGCAGACGACATCA
ACTGGATATGTTTGCCCAAGCCACAAATTGAGGCCTCTCTCGTTCCGTCT
GCCTCTGATTTTGTGGTCTTTCCTCAGCACAGCACATTTTATGTTGGCAT
CAATCATTCAGAGCCGCGAATATTAGCAAAATCAATATTTTTTCGTGGCA
CAATATACTCAAGATCTTATCAATTTCAACAAAAAATCAAAAAAACTTTT
ACAAGAAAGTTTGTCGAATTTGTCGACCTCAATTCTTCATATCCTCCACA
GATTGAGCTTCAGGGGAATAAATGCAAGAAAGATTATTGCATTGTTAACA
GCAATTTACAGAGAATGGATGCCATATGCAAATACGTGACTTGCCAAACA
CCAAAATGCGGCCATAGCGTGAAACCGAAGGGCAGCTGTTGCGATTTATG
CGGTCACCTGTTAACAGTTGGGTTATCAAAGGAGTCAAATAAGACCAAAA
TAGGAGACTGGTTAAACAAATATCTAAATTCTCAATTTCAGAAAAAGATA
ATATTTGATCTGCTCTCGGTGGAAATCAGAGAATTTCAACTGACAATTTC
CCCTTTGATAACTGAATCGGAACAATTTGATAAAATTATTCTCGAAATTG
TGGAAAATATTAAATCAAGACCGACGTCAGAAGGAGTGATTTTAGTCAAG
TGTACAAGTTGCAAAACTCCGAAAGCATTTTCAAGAGACCCACAAAAATC
CAAATACTGGATATCCTTGCTGGTCATTGGGGTATTCATGCTGTTCGTCA
TCGCCCTGTTGTTATACATGAATCGTAAAAGTGGGACATCAAGATTTCAG
TTCAATTTTAACCTGCCAAAAATGCCGAAATTTCAATTTCCTCGAATGAA
TTTTGACAATTTTAGATCAAACTTTCCTAGAGTGAGACTTCACGAGACAT
TTGACGAGAATTCTCGCGGCCACTCGGAATTTGTTGAAGCGATTGAGCTG
CCAGACACTGGCACCGAGGTGTCGTTTGATAACCCGATCTATGTTGCCGA
AATCGACGACTCTAAGCAATGAAGACCTTTTTCGTTTATATTTTGAAATC
TTTGATTAATTTAATATAAAATAAATTTTTTTAGTTTTTAAAAAAAA
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protein sequence of SMED30031417-orf-1

>SMED30031417-orf-1 ID=SMED30031417-orf-1|Name=SMED30031417-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=461bp
MLNLLILICLFVSGNAVKLFIANTNFNNSKNWKEAQVPCSGNHLVFPKRI
SRVFINAMSAPLQVMDIDFPINGEIILGNSLQITAFGNQVDDCDSTVSHF
TKVAPDDWYNPLNWCQADDINWICLPKPQIEASLVPSASDFVVFPQHSTF
YVGINHSEPRILAKSIFFRGTIYSRSYQFQQKIKKTFTRKFVEFVDLNSS
YPPQIELQGNKCKKDYCIVNSNLQRMDAICKYVTCQTPKCGHSVKPKGSC
CDLCGHLLTVGLSKESNKTKIGDWLNKYLNSQFQKKIIFDLLSVEIREFQ
LTISPLITESEQFDKIILEIVENIKSRPTSEGVILVKCTSCKTPKAFSRD
PQKSKYWISLLVIGVFMLFVIALLLYMNRKSGTSRFQFNFNLPKMPKFQF
PRMNFDNFRSNFPRVRLHETFDENSRGHSEFVEAIELPDTGTEVSFDNPI
YVAEIDDSKQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
Vocabulary: INTERPRO
TermDefinition
IPR026112AMN
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026112AmnionlessPFAMPF14828Amnionlesscoord: 17..451
e-value: 4.0E-51
score: 174.2
IPR026112AmnionlessPANTHERPTHR14995AMNIONLESScoord: 2..457
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..16
score: 0.893
NoneNo IPR availableTMHMMTMhelixcoord: 356..378