WD_REPEATS_REGION domain-containing protein

Overview
NameWD_REPEATS_REGION domain-containing protein
Smed IDSMED30031408
Length (bp)537
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of WD_REPEATS_REGION domain-containing protein (SMED30031408) t-SNE clustered cells

Violin plots show distribution of expression levels for WD_REPEATS_REGION domain-containing protein (SMED30031408) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30031408

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30031408h1SMcG0003267 SmedASXL_012755SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X1 cellSMED30031408h1SMcG0003267 SmedASXL_004094SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30031408h1SMcG0003267 dd_Smed_v4_29867_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30031408h1SMcG0003267 dd_Smed_v4_29867_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30031408h1SMcG0003267 dd_Smed_v4_74366_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30031408h1SMcG0003267 dd_Smed_v4_74366_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
zeta neoblastSMED30031408h1SMcG0003267 dd_Smed_v4_29867_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
zeta neoblastSMED30031408h1SMcG0003267 dd_Smed_v4_21908_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Celegans
Match: T05C3.2 (pep chromosome:WBcel235:V:5493747:5503010:1 gene:WBGene00020254.1 transcript:T05C3.2.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T05C3.2)

HSP 1 Score: 129.028 bits (323), Expect = 1.015e-34
Identity = 63/178 (35.39%), Postives = 103/178 (57.87%), Query Frame = 2 
Query:    2 YYMSVTEREVIHGLINDDNPNQHCLCFIRELTNISMDHLESVKKYLDILTGKPDKEAQGLLKKLKFEKVTKILNENNIIRNSIPWIDPDGMNFKDHEDYLIQFSDTFVSKLKNLIENGVQNQRKVTEDYLFYEIFTHLDSVHKFNKDFFGHEDLIEDINKTINDSQQNKPIFLRGPPG 535
            Y+MSVTEREV+HG++   NPN+HCLC+IR + NI++  +++  K++DI     + EAQ LL  L+ E+V   L   NI R+++ W+  DG++ + H +Y+ QF   F   + ++++N ++   +   D LF E+ THL +    +  FFG  + + D  K         PI L+G  G
Sbjct:  292 YFMSVTEREVVHGILKVKNPNEHCLCYIRHINNIALSQMKTASKFVDIAHNHVNSEAQKLLANLRDERVPAKLAMQNIRRSTVEWVGRDGIDVQYHAEYIRQFCTDFYKSITSMVDNAMEKHARF-RDQLFTEVLTHLTTGITVSNMFFGRNEQL-DCAKQYVLGNSTLPIILQGENG 467
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Fly
Match: CG10185 (gene:FBgn0038397 transcript:FBtr0083235)

HSP 1 Score: 103.219 bits (256), Expect = 1.429e-25
Identity = 55/179 (30.73%), Postives = 98/179 (54.75%), Query Frame = 2 
Query:    2 YYMSVTEREVIHGLINDDNPNQHCLCFIRELTNISMDHLESVKKYLDILTGKPDKEAQGLLKKLKFEKVTKILNENNIIRNSIPWIDPDGMNFKDHEDYLIQFSDTFVSKLKNLIENGVQNQRKVTEDYLFYEIFTHLDSVHKFNKDFFGHEDLIEDINK-TINDSQQNKPIFLRGPPG 535
            Y+MSVTEREVI+G++N  N   HCL ++R + NI++ +L+    ++DI+    D E+  LL  L+  ++   +   N  + ++ WI  +G++ + HE+YL  F   F   +  L++  ++ +    +  +  EI  HL + +   K F+G E+  E I +  + DS  +KP+ L G  G
Sbjct:  216 YFMSVTEREVINGILNVKNTKNHCLSYVRYINNINLQNLKKASLFVDIINRSLDTESAKLLSDLRDVRLPAKIEAVNAQKYTVEWIGREGLDIETHEEYLNHFISHFYKNVVKLVDRAMRKEDSSAQGQIVTEILQHLHACNNSVKIFYGREESCERIKRYMLGDS--DKPLVLFGDGG 392
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Zebrafish
Match: nwd1 (NACHT and WD repeat domain containing 1 [Source:ZFIN;Acc:ZDB-GENE-071116-8])

HSP 1 Score: 50.8322 bits (120), Expect = 1.967e-7
Identity = 53/191 (27.75%), Postives = 89/191 (46.60%), Query Frame = 2 
Query:    2 YYMSVTEREVIHGLINDDNPNQHCLCFIRELTNISM-DHLESVKKYLDILT--GKPDKEAQGLLKKLKFEKVTKILNENNI----IRNSIPWIDPDGMNFKDHEDYLIQFSDTFVSKLKNLIENGVQNQRKVTED----YLFYEIFTHLDSVHKFNKDFFGHEDLIEDINKTINDSQ--QNKPIFLRGPPG 535
            ++ SV E E+ HG +     +   + F REL  +S  D  ++  K+L  +T  G  D +A+ LL  LK     +I N   +    +  S   +DP   + K+H++YL +  +   S+LK LI          + D    +L  EIF H     +    F G +D++  I+ T+ +S    + P+ + GPPG
Sbjct:  222 FFKSVLEYEMEHGFLRTQR-DASAVLFFRELPRLSKRDCKKNFAKFLLDVTADGLLDTQARELLTDLK----QRICNTGRLQPLCMELSKGAMDP---SRKEHKEYLGKLCEQVTSQLKELISRNALQTSGTSVDPVWSWLKQEIFHHAKLSEEKCAVFKGRDDILGKISITLWESNDVSHGPLLVHGPPG 404
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Xenopus
Match: ppp1r3c.1 (protein phosphatase 1, regulatory (inhibitor) subunit 3C, gene 1 [Source:Xenbase;Acc:XB-GENE-969181])

HSP 1 Score: 46.9802 bits (110), Expect = 7.034e-6
Identity = 50/190 (26.32%), Postives = 87/190 (45.79%), Query Frame = 2 
Query:    2 YYMSVTEREVIHGLINDDNPNQHCLCFIRELTNISMDHLESVKKYLDILT-GKPDKEAQGLLKKLKFEKVT--KILNENNIIRNSIPWIDPDGMNFKDHEDYLIQFSDTFVSKLKNLI----ENGVQNQRKVTEDYLFY---EIFTHLDSVHKFNKDFFGHEDLIEDINKTIND--SQQNKPIFLRGPPG 535
            Y+ SVTE E+  GL+      +    F+RE+ +++   +  +   LD+   G  D EAQ LL+ LK   +T  K   + +++  SI          K+   YL +  D  ++ + + I     +G   Q +    +L +   E+  HL S  K ++ F G +DL++ I   I +  SQ   P+ + G  G
Sbjct:  159 YFKSVTESEIECGLLACGANKEGSTIFLREVKDLNQCQISGL---LDVREDGSTDTEAQDLLQNLKNRILTDHKDCVKTHVVEMSIQ---------KNKMAYLHKLCDQAIAVVNHQILKCLPSGNAGQSQSEHPWLGWLRQEVIHHLSSSQKKSQVFSGRQDLLQKIMSYIQERHSQVYPPLIIHGVSG 336
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Mouse
Match: Nwd1 (NACHT and WD repeat domain containing 1 [Source:MGI Symbol;Acc:MGI:2442268])

HSP 1 Score: 59.3066 bits (142), Expect = 2.858e-10
Identity = 50/190 (26.32%), Postives = 95/190 (50.00%), Query Frame = 2 
Query:    2 YYMSVTEREVIHGLINDDNPNQHCLCFIRELTNISMDHLE--SVKKYLDILTGKPDKEAQGLLKKLKFEKVTKILNEN--NIIRNSIPW----IDPDGMNFKDHEDYLIQFSDTFVSKLKNLIENGVQNQRKVTED--YLFYEIFTHLDSVHKFNKDFFGHEDLIEDINKTI--NDSQQNKPIFLRGPPG 535
            Y+ SV E E+  GL++    +Q    F+R++ +++   L+  S+K    ++ G  D  AQ LL  LK     +IL+     +  + + W    ++P     K H  YL Q S+ FV++  + +   ++      ++  +L+ EI  HL    +  K F GH++L+  + + +  ++S+ + P+ L GPPG
Sbjct:  163 YHRSVIEWEIELGLLSSARGDQGATVFLRDVQDLNKHILDDCSLKMVDRLVDGCLDTNAQSLLSGLK----GRILDAQPGALKSHHLSWSRDLVNPKN---KAHARYLKQLSEQFVARTNHQVLEQLRELELARQELGWLYQEIRHHLWQSTESTKVFCGHQELLAQLRQQLRQDESRTHTPLVLFGPPG 345
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Mouse
Match: Nwd1 (NACHT and WD repeat domain containing 1 [Source:MGI Symbol;Acc:MGI:2442268])

HSP 1 Score: 59.3066 bits (142), Expect = 2.863e-10
Identity = 50/190 (26.32%), Postives = 95/190 (50.00%), Query Frame = 2 
Query:    2 YYMSVTEREVIHGLINDDNPNQHCLCFIRELTNISMDHLE--SVKKYLDILTGKPDKEAQGLLKKLKFEKVTKILNEN--NIIRNSIPW----IDPDGMNFKDHEDYLIQFSDTFVSKLKNLIENGVQNQRKVTED--YLFYEIFTHLDSVHKFNKDFFGHEDLIEDINKTI--NDSQQNKPIFLRGPPG 535
            Y+ SV E E+  GL++    +Q    F+R++ +++   L+  S+K    ++ G  D  AQ LL  LK     +IL+     +  + + W    ++P     K H  YL Q S+ FV++  + +   ++      ++  +L+ EI  HL    +  K F GH++L+  + + +  ++S+ + P+ L GPPG
Sbjct:  204 YHRSVIEWEIELGLLSSARGDQGATVFLRDVQDLNKHILDDCSLKMVDRLVDGCLDTNAQSLLSGLK----GRILDAQPGALKSHHLSWSRDLVNPKN---KAHARYLKQLSEQFVARTNHQVLEQLRELELARQELGWLYQEIRHHLWQSTESTKVFCGHQELLAQLRQQLRQDESRTHTPLVLFGPPG 386
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Mouse
Match: Nwd1 (NACHT and WD repeat domain containing 1 [Source:MGI Symbol;Acc:MGI:2442268])

HSP 1 Score: 59.3066 bits (142), Expect = 2.863e-10
Identity = 50/190 (26.32%), Postives = 95/190 (50.00%), Query Frame = 2 
Query:    2 YYMSVTEREVIHGLINDDNPNQHCLCFIRELTNISMDHLE--SVKKYLDILTGKPDKEAQGLLKKLKFEKVTKILNEN--NIIRNSIPW----IDPDGMNFKDHEDYLIQFSDTFVSKLKNLIENGVQNQRKVTED--YLFYEIFTHLDSVHKFNKDFFGHEDLIEDINKTI--NDSQQNKPIFLRGPPG 535
            Y+ SV E E+  GL++    +Q    F+R++ +++   L+  S+K    ++ G  D  AQ LL  LK     +IL+     +  + + W    ++P     K H  YL Q S+ FV++  + +   ++      ++  +L+ EI  HL    +  K F GH++L+  + + +  ++S+ + P+ L GPPG
Sbjct:  163 YHRSVIEWEIELGLLSSARGDQGATVFLRDVQDLNKHILDDCSLKMVDRLVDGCLDTNAQSLLSGLK----GRILDAQPGALKSHHLSWSRDLVNPKN---KAHARYLKQLSEQFVARTNHQVLEQLRELELARQELGWLYQEIRHHLWQSTESTKVFCGHQELLAQLRQQLRQDESRTHTPLVLFGPPG 345
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. UniProt/SwissProt
Match: sp|A6H603|NWD1_MOUSE (NACHT domain- and WD repeat-containing protein 1 OS=Mus musculus OX=10090 GN=Nwd1 PE=2 SV=2)

HSP 1 Score: 59.3066 bits (142), Expect = 2.003e-9
Identity = 50/190 (26.32%), Postives = 95/190 (50.00%), Query Frame = 2 
Query:    2 YYMSVTEREVIHGLINDDNPNQHCLCFIRELTNISMDHLE--SVKKYLDILTGKPDKEAQGLLKKLKFEKVTKILNEN--NIIRNSIPW----IDPDGMNFKDHEDYLIQFSDTFVSKLKNLIENGVQNQRKVTED--YLFYEIFTHLDSVHKFNKDFFGHEDLIEDINKTI--NDSQQNKPIFLRGPPG 535
            Y+ SV E E+  GL++    +Q    F+R++ +++   L+  S+K    ++ G  D  AQ LL  LK     +IL+     +  + + W    ++P     K H  YL Q S+ FV++  + +   ++      ++  +L+ EI  HL    +  K F GH++L+  + + +  ++S+ + P+ L GPPG
Sbjct:  163 YHRSVIEWEIELGLLSSARGDQGATVFLRDVQDLNKHILDDCSLKMVDRLVDGCLDTNAQSLLSGLK----GRILDAQPGALKSHHLSWSRDLVNPKN---KAHARYLKQLSEQFVARTNHQVLEQLRELELARQELGWLYQEIRHHLWQSTESTKVFCGHQELLAQLRQQLRQDESRTHTPLVLFGPPG 345
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. TrEMBL
Match: A0A267FFL7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig027966g1 PE=4 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 2.380e-32
Identity = 64/178 (35.96%), Postives = 105/178 (58.99%), Query Frame = 2 
Query:    2 YYMSVTEREVIHGLINDDNPNQHCLCFIRELTNISMDHLESVKKYLDILTGKPDKEAQGLLKKLKFEKVTKILNENNIIRNSIPWIDPDGMNFKDHEDYLIQFSDTFVSKLKNLIENGVQNQRKVTEDYLFYEIFTHLDSVHKFNKDFFGHEDLIEDINKTINDSQQNKPIFLRGPPG 535
            Y+MSVTERE IHG++  D+ ++H L F+R+L  IS+++  + + ++D+   + D+EAQ L+  L+  K+ + L  ++IIR S PW+DP G++   H++Y+ QF DTF ++L  LI+ G +  R V++D    EI  HL    K  + F G E  ++ I     D    +   + G PG
Sbjct: 1018 YFMSVTERENIHGILTADSNHRHTLAFLRQLEGISLENWRTARNFIDMSGPEVDREAQRLMDDLRDRKIPERLRASSIIRYSQPWVDPSGIHLDTHKEYIDQFCDTFYTRLVKLIDRGARRNRLVSKDATLAEILQHLLVCAKLCELFQGREADLQKIRNYCRDPDNRQIFIIYGDPG 1195
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. TrEMBL
Match: A0A267GGQ3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig005707g1 PE=4 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 3.094e-32
Identity = 63/178 (35.39%), Postives = 105/178 (58.99%), Query Frame = 2 
Query:    2 YYMSVTEREVIHGLINDDNPNQHCLCFIRELTNISMDHLESVKKYLDILTGKPDKEAQGLLKKLKFEKVTKILNENNIIRNSIPWIDPDGMNFKDHEDYLIQFSDTFVSKLKNLIENGVQNQRKVTEDYLFYEIFTHLDSVHKFNKDFFGHEDLIEDINKTINDSQQNKPIFLRGPPG 535
            Y+MSVTERE IHG++  ++ ++H L F+R+L  IS+++  + + ++D+   + D+EAQ L+  L+  K+ + L  ++IIR S PW+DP G++   H++Y+ QF DTF ++L  LI+ G +  R V++D    EI  HL    K  + F G E  ++ I     D    +   + G PG
Sbjct:  916 YFMSVTERENIHGILTAESNHRHTLAFLRQLEGISLENWRTARNFIDMSGPEVDREAQRLMDDLRDRKIPECLRASSIIRYSQPWVDPSGIHLDTHKEYIDQFCDTFYTRLVKLIDRGARRNRLVSKDATLAEILQHLLVCAKLCELFQGREADLQKIRNYCRDPDNRQIFIIYGDPG 1093
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. TrEMBL
Match: A8X980 (Protein CBG09382 OS=Caenorhabditis briggsae OX=6238 GN=CBG09382 PE=4 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 4.015e-32
Identity = 64/178 (35.96%), Postives = 104/178 (58.43%), Query Frame = 2 
Query:    2 YYMSVTEREVIHGLINDDNPNQHCLCFIRELTNISMDHLESVKKYLDILTGKPDKEAQGLLKKLKFEKVTKILNENNIIRNSIPWIDPDGMNFKDHEDYLIQFSDTFVSKLKNLIENGVQNQRKVTEDYLFYEIFTHLDSVHKFNKDFFGHEDLIEDINKTINDSQQNKPIFLRGPPG 535
            Y+MSVTEREV+HG++   NPN+HCLC+IR + NI++  +++  K++DI     + EAQ LL  L+ E+V   L   NI R+++ W+  DG++ + H +Y+ QF   F + +  +++N +    K   D LF E+ THL +    +  FFG ++ + +  K       N PI L+G  G
Sbjct:  292 YFMSVTEREVVHGILKAKNPNEHCLCYIRHINNIALSQMKTASKFVDIAHNHVNSEAQKLLANLRDERVPAKLAMQNIRRSTVEWVGRDGIDVQYHAEYIRQFCSDFYNSITTMVDNAMAKHEKY-RDQLFTEVLTHLTTGSTVSDMFFGRDEQL-NCAKQYVLGNSNLPIILQGENG 467
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. TrEMBL
Match: A0A261BUF8 (Uncharacterized protein (Fragment) OS=Caenorhabditis latens OX=1503980 GN=FL83_12038 PE=4 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 4.388e-32
Identity = 64/178 (35.96%), Postives = 105/178 (58.99%), Query Frame = 2 
Query:    2 YYMSVTEREVIHGLINDDNPNQHCLCFIRELTNISMDHLESVKKYLDILTGKPDKEAQGLLKKLKFEKVTKILNENNIIRNSIPWIDPDGMNFKDHEDYLIQFSDTFVSKLKNLIENGVQNQRKVTEDYLFYEIFTHLDSVHKFNKDFFGHEDLIEDINKTINDSQQNKPIFLRGPPG 535
            Y+MSVTEREV+HG++   NPN+HCLC+IR + NI++  +++  K++DI     + EAQ LL  L+ E+V   L   NI R+++ W+  DG++ + H +Y+ QF   F + + ++++N ++   +   D LF E+ THL +    +  FFG  + + D  K       N PI L+G  G
Sbjct:  701 YFMSVTEREVVHGILKVKNPNEHCLCYIRHINNIALSQMKTASKFVDIAHNHVNSEAQKLLANLRDERVPAKLAMQNIRRSTVEWVGRDGIDVQYHAEYIRQFCTDFYTSITSMVDNAMEKHARF-RDQLFTEVLTHLTTGITVSNMFFGRNEQL-DCAKQYVLGNSNLPIILQGENG 876
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. TrEMBL
Match: A0A260YQK4 (Uncharacterized protein (Fragment) OS=Caenorhabditis remanei OX=31234 GN=FL82_20364 PE=4 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 4.482e-32
Identity = 64/178 (35.96%), Postives = 105/178 (58.99%), Query Frame = 2 
Query:    2 YYMSVTEREVIHGLINDDNPNQHCLCFIRELTNISMDHLESVKKYLDILTGKPDKEAQGLLKKLKFEKVTKILNENNIIRNSIPWIDPDGMNFKDHEDYLIQFSDTFVSKLKNLIENGVQNQRKVTEDYLFYEIFTHLDSVHKFNKDFFGHEDLIEDINKTINDSQQNKPIFLRGPPG 535
            Y+MSVTEREV+HG++   NPN+HCLC+IR + NI++  +++  K++DI     + EAQ LL  L+ E+V   L   NI R+++ W+  DG++ + H +Y+ QF   F + + ++++N ++   +   D LF E+ THL +    +  FFG  + + D  K       N PI L+G  G
Sbjct:  701 YFMSVTEREVVHGILKVKNPNEHCLCYIRHINNIALSQMKTASKFVDIAHNHVNSEAQKLLANLRDERVPAKLAMQNIRRSTVEWVGRDGIDVQYHAEYIRQFCTDFYTSITSMVDNAMEKHARF-RDQLFTEVLTHLTTGITVSNMFFGRNEQL-DCAKQYVLGNSNLPIILQGENG 876
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Cavefish
Match: nwd1 (NACHT and WD repeat domain containing 1 [Source:ZFIN;Acc:ZDB-GENE-071116-8])

HSP 1 Score: 45.8246 bits (107), Expect = 9.058e-6
Identity = 46/167 (27.54%), Postives = 78/167 (46.71%), Query Frame = 2 
Query:   47 NDDNPNQHCLCFIRELTNISM-DHLESVKKYLDILT-GKPDKEAQGLLKKLKFEKVTKILNENNIIRNSIPWIDPDGMNFKDHEDYLIQFSDTFVSKLKNLIENGVQNQRKVTEDYLFYEIFTHLDSVHKFNKDFFGHEDLIEDINKTINDS--QQNKPIFLRGPPG 535
            + DNP+   + F+R+L  +   +  +   K+LD+   G  DKEAQ LL KLK E++     ++    +    +    ++ K+H++YL  F    VS+LK  I             +L  E+  H +   K    F G + L+  I   + +S   Q+ P+ + GPPG
Sbjct:  188 SQDNPS--AVIFVRDLPPLKKSEQQKHTLKFLDVTADGLLDKEAQELLTKLK-ERIHSACADDPGFHHI--ELSKGAIDRKEHKEYLENFCKQIVSQLKGKISRQTAPPAVPGWLWLQQELLHHANLSKKQCAVFCGRDGLLGKICLVMWESLNMQHGPLVVSGPPG 349
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Nematostella
Match: EDO31117 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SYV4])

HSP 1 Score: 85.8853 bits (211), Expect = 6.366e-20
Identity = 47/123 (38.21%), Postives = 72/123 (58.54%), Query Frame = 2 
Query:    2 YYMSVTEREVIHGLINDDNPNQHCLCFIRELTNISMD-HLESVKKYLDILTGKPDKEAQGLLKKLKFEKVTKILNENNIIRNSIPWIDPDGMNFKD--HEDYLIQFSDTFVSKLKNLIENGVQ 361
            YY SVTE E+ HG++N  +P    LC+IRE+ +I+++   E +  Y DI  G  D EAQ  L +LK ++V   L   N+++ S+PW +  G+N  D  H +YL  F + F S L  LI+  ++
Sbjct:  220 YYKSVTEHEIEHGILNCADPEAQTLCYIREIEDINLNIKDEKIPLYTDIADGGHDAEAQEHLFRLKRQRVLDKLKAENVVQYSVPW-NSRGINASDRRHHEYLENFCNRFESDLTRLIDRSLE 341
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Nematostella
Match: EDO34994 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SMT6])

HSP 1 Score: 58.9214 bits (141), Expect = 9.842e-11
Identity = 52/191 (27.23%), Postives = 89/191 (46.60%), Query Frame = 2 
Query:   11 SVTEREVIHGLINDDNPNQHCLCFIRELTNISMDHLESVKKYLDILT---GKPDKEAQGLLKKLKFEKVTKILNENNIIRNSIPW----IDPDGMNFKDHEDYLIQFSDTFVSKLKNLIENGVQ--NQRKVTEDYLFYEIFTHLDSVHKFNKDFFGHEDLIEDINKTIN-------DSQQNKPIFLRGPPG 535
            +VTE E+  G++   +P + C  F R + ++   H+   + +L I T   G  D EAQ LL  LK + +   L  +N+    + W    I P+  N  +H  Y+ +  +     L++LI N ++  N  +VT D +F E   HL       + F+G ED++      +N       D  +N+P+ + G  G
Sbjct:  143 TVTESEIRQGILASADPARECCWFSRNIRDLE-KHVADGRAHLFIDTTREGDVDLEAQRLLGNLKKDLLHCYLPLDNVKTYDVTWSRDGISPNLNN--EHRAYIERMCEDVQIVLQHLITNAIERRNSSEVT-DPMFEEALQHLSFCQSKCEQFYGREDVLLSCRLILNMRSYDVLDVCRNRPLVIYGESG 329
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Nematostella
Match: EDO40517 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S6Z9])

HSP 1 Score: 49.2914 bits (116), Expect = 2.031e-7
Identity = 43/133 (32.33%), Postives = 65/133 (48.87%), Query Frame = 2 
Query:  161 DKEAQGLLKKLKFEKVTKILNENNIIRNSIPW----IDPDGMNFKDHEDYLIQFSDTFVSKLKNLIENGVQNQRKVTE----DYLFYEIFTHLDSVHKFNKDFFGHEDLIEDINKTINDSQQNKPIFLRGPPG 535
            DK AQ LLK L+ E +   L+++NI    I W    + P+G N   H  YL +    F   +K  I + +  +R   E    ++LF E+  H    +   ++F G ED++E I   I+ S Q KP+ L G  G
Sbjct:  207 DKPAQYLLKNLQSE-IRSTLDKSNIFSYDIKWTSEGVTPEG-NLT-HGQYLGKLCTEFYDVMKKKITDAI-TKRAAGEVRDKEHLFQEVAQHATFCNLKCENFHGREDILEKIRTYISGSGQ-KPLVLYGDSG 334
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Nematostella
Match: EDO47108 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNA9])

HSP 1 Score: 45.8246 bits (107), Expect = 3.479e-6
Identity = 32/122 (26.23%), Postives = 58/122 (47.54%), Query Frame = 2 
Query:  152 GKPDKEAQGLLKKLKFEKVTKILNENNIIRNSIPW----IDPDGMNFKDHEDYLIQFSDTFVSKLKNLIENGVQNQRKV-TEDYLFYEIFTHLDSVHKFNKDFFGHEDLIEDINKTINDSQQ 502
            GK D EA  LL   + + + K L  +N+ +  + W    IDP     ++H  Y+ +   +F   L  +I   V  + +V  +D L  E+F+HL    K  + F G E+ ++ + + +  + Q
Sbjct:  186 GKIDMEAYRLLSHFREKILPKALPSSNVKQFDVKWAENGIDPS--ESEEHAQYIEELCTSFYETLTGMIGTAVSERAEVEVKDPLAEEVFSHLTFCLKKCEAFHGREEFLDSVKQALLSNGQ 305
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Planmine SMEST
Match: SMESG000018097.1 (SMESG000018097.1)

HSP 1 Score: 206.068 bits (523), Expect = 1.501e-61
Identity = 97/98 (98.98%), Postives = 98/98 (100.00%), Query Frame = 2 
Query:    2 YYMSVTEREVIHGLINDDNPNQHCLCFIRELTNISMDHLESVKKYLDILTGKPDKEAQGLLKKLKFEKVTKILNENNIIRNSIPWIDPDGMNFKDHED 295
            YYMSVTEREVIHGLINDDNPNQHCLCFIRELTNISMDHLESVKKYLDILTGKPDKEAQGLLKKLKFEKVTKILNENNIIRNSIPWIDPDGMNFKDHE+
Sbjct:   81 YYMSVTEREVIHGLINDDNPNQHCLCFIRELTNISMDHLESVKKYLDILTGKPDKEAQGLLKKLKFEKVTKILNENNIIRNSIPWIDPDGMNFKDHEE 178
The following BLAST results are available for this feature:
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
T05C3.21.015e-3435.39pep chromosome:WBcel235:V:5493747:5503010:1 gene:W... [more]
back to top
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CG101851.429e-2530.73gene:FBgn0038397 transcript:FBtr0083235[more]
back to top
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
nwd11.967e-727.75NACHT and WD repeat domain containing 1 [Source:ZF... [more]
back to top
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ppp1r3c.17.034e-626.32protein phosphatase 1, regulatory (inhibitor) subu... [more]
back to top
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Nwd12.858e-1026.32NACHT and WD repeat domain containing 1 [Source:MG... [more]
Nwd12.863e-1026.32NACHT and WD repeat domain containing 1 [Source:MG... [more]
Nwd12.863e-1026.32NACHT and WD repeat domain containing 1 [Source:MG... [more]
back to top
BLAST of WD_REPEATS_REGION domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|A6H603|NWD1_MOUSE2.003e-926.32NACHT domain- and WD repeat-containing protein 1 O... [more]
back to top
BLAST of WD_REPEATS_REGION domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267FFL72.380e-3235.96Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267GGQ33.094e-3235.39Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A8X9804.015e-3235.96Protein CBG09382 OS=Caenorhabditis briggsae OX=623... [more]
A0A261BUF84.388e-3235.96Uncharacterized protein (Fragment) OS=Caenorhabdit... [more]
A0A260YQK44.482e-3235.96Uncharacterized protein (Fragment) OS=Caenorhabdit... [more]
back to top
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
nwd19.058e-627.54NACHT and WD repeat domain containing 1 [Source:ZF... [more]
back to top
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 4
Match NameE-valueIdentityDescription
EDO311176.366e-2038.21Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO349949.842e-1127.23Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO405172.031e-732.33Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO471083.479e-626.23Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of WD_REPEATS_REGION domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000018097.11.501e-6198.98SMESG000018097.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031408 ID=SMED30031408|Name=WD_REPEATS_REGION domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=537bp
TTATTATATGTCAGTTACCGAAAGAGAAGTTATTCATGGATTAATTAATG
ATGACAACCCAAATCAACATTGTTTATGTTTTATCAGAGAACTGACTAAC
ATATCAATGGATCATTTAGAGTCTGTGAAAAAATACTTAGATATTCTTAC
CGGAAAACCAGATAAGGAAGCGCAAGGTCTTCTCAAAAAGTTAAAATTTG
AAAAGGTTACAAAAATCTTGAATGAAAATAATATTATTAGGAATTCTATA
CCTTGGATTGATCCAGATGGTATGAATTTTAAAGATCATGAAGATTACCT
GATTCAATTCTCGGATACTTTTGTGTCGAAACTAAAGAATCTGATTGAAA
ACGGAGTCCAGAATCAAAGAAAAGTAACAGAAGATTATTTATTTTATGAG
ATATTTACTCATCTAGACTCAGTACATAAATTTAATAAAGATTTTTTTGG
ACATGAAGATTTAATTGAAGATATTAATAAAACTATAAATGATTCTCAAC
AAAACAAGCCAATATTTTTGAGAGGACCTCCGGGAAG
back to top

protein sequence of SMED30031408-orf-1

>SMED30031408-orf-1 ID=SMED30031408-orf-1|Name=SMED30031408-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=178bp
YYMSVTEREVIHGLINDDNPNQHCLCFIRELTNISMDHLESVKKYLDILT
GKPDKEAQGLLKKLKFEKVTKILNENNIIRNSIPWIDPDGMNFKDHEDYL
IQFSDTFVSKLKNLIENGVQNQRKVTEDYLFYEIFTHLDSVHKFNKDFFG
HEDLIEDINKTINDSQQNKPIFLRGPPG
back to top
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR19871BETA TRANSDUCIN-RELATED PROTEINcoord: 1..174