SMED30031362

Overview
NameSMED30031362
Smed IDSMED30031362
Length (bp)1929
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30031362 (SMED30031362) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30031362 (SMED30031362) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30031362 (SMED30031362) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30031362 (SMED30031362) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30031362

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 15

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30031362h1SMcG0006639 SmedASXL_017381SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30031362h1SMcG0006639 SmedASXL_017382SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
pharynxSMED30031362h1SMcG0006639 dd_Smed_v4_1071_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30031362h1SMcG0006639 dd_Smed_v4_1071_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30031362h1SMcG0006639 dd_Smed_v4_1071_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30031362h1SMcG0006639 dd_Smed_v4_1071_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30031362h1SMcG0006639 dd_Smed_v4_1071_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031362h1SMcG0006639 dd_Smed_v4_1071_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30031362h1SMcG0006639 dd_1071dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult fluorescence in situ hybridization evidence
head regionSMED30031362h1SMcG0006639 dd_Smed_v6_1071_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
head regionSMED30031362h1SMcG0006639 OX_Smed_1.0.09131ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
head regionSMED30031362h1SMcG0006639 OX_Smed_1.0.14492ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
pharynxSMED30031362h1SMcG0006639 dd_Smed_v6_1071_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynx progenitor cellSMED30031362h1SMcG0006639 dd_Smed_v6_1071_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30031362h1SMcG0006639 dd_Smed_v4_1071_0_1dd_Smed_v4PMID:30962434
Cote et al., 2019
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30031362 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.007402:173..5644 -9340
Homology
BLAST of SMED30031362 vs. Planmine SMEST
Match: SMESG000033673.1 (SMESG000033673.1)

HSP 1 Score: 698.353 bits (1801), Expect = 0.000e+0
Identity = 359/368 (97.55%), Postives = 365/368 (99.18%), Query Frame = 3
Query:  723 KLLALVFLNKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRTSHRNCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTLNGKCQIKSLSIKGGKCGCLGSTFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICPTNSMVXXXXXXXXXXXXVISLFEKSYNQCKLVKFSFKWTKCIIPISGKNCSKIKRLTMSNCQKGKAYIIFTKSAEKPVNGTCGLNSSKIAQVNCTDKCERTIRPKLVAGICEKQIQLVFFIASRPQGKQCLHKINYLGKIGCKNC 1826
            +++   FLNKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRTSHRNCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTT+NGKCQ+KSLSIKGGKCGCLGSTFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICPTNS+VLSSKCLKKRKLRVISLFEKSYNQCKLVKFSFKWTKCIIPISGKNCSKIKRLTMSNCQKGKAYIIFTKSAEKPVNGTCGLNSSKIAQVNCTDKCERTIRPKLVAGICEKQIQLVFFIASRPQGKQCLHKINYLGKIGCKNC
Sbjct: 2098 QIVGPCFLNKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRTSHRNCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTVNGKCQVKSLSIKGGKCGCLGSTFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICPTNSVVLSSKCLKKRKLRVISLFEKSYNQCKLVKFSFKWTKCIIPISGKNCSKIKRLTMSNCQKGKAYIIFTKSAEKPVNGTCGLNSSKIAQVNCTDKCERTIRPKLVAGICEKQIQLVFFIASRPQGKQCLHKINYLGKIGCKNC 2465          

HSP 2 Score: 488.419 bits (1256), Expect = 0.000e+0
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 2
Query:    2 GNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQIVGPCFL 745
            GNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQIVGPCFL
Sbjct: 1858 GNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQIVGPCFL 2105          

HSP 3 Score: 358.607 bits (919), Expect = 1.891e-122
Identity = 185/247 (74.90%), Postives = 205/247 (83.00%), Query Frame = 2
Query:    2 GNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQI-VGPC 739
            GN TKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEG KCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRC ++ Y++++C  N K T LIR  ++  N  C+R +  +  LPC+CY N   N+TV  G  CQNG+ SRV+EL VEK+  C K  FKLRSI+C   C+R  ++ VG C
Sbjct: 1114 GNITKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGNKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCQKTSYKVEKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCRKPCDRRRKVEVGKC 1360          

HSP 4 Score: 101.679 bits (252), Expect = 1.891e-122
Identity = 62/222 (27.93%), Postives = 117/222 (52.70%), Query Frame = 3
Query:  747 NKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRT----SHRNCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTLNGKCQIKSLSIKGGKCGCLGS---TFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICP 1391
            N+R + VL+ ++  N  C WK+++S FV C   CP+P+ K + CI  ++ + R++++E G KC     +L  + CQ C + S Y + +C K +K ++LVRT    +++ C +K   +  + C C G     +NV     C +G   + L      N KC   +  ++  +C    S      ++ CV+  R++++    + G +C+ K+V S+ + C+++CP
Sbjct: 1363 NQRSLTVLRAVREKN-RCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGC-QNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQSGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNLCP 1582          

HSP 5 Score: 319.316 bits (817), Expect = 7.011e-111
Identity = 166/247 (67.21%), Postives = 194/247 (78.54%), Query Frame = 2
Query:    2 GNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQI-VGPC 739
            GNTTKNINVVVGKQCQ+GYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKN CPLPKE+++RCI+G Q VM++TF  VGN C     RV + KCQ CP+  Y++++C  N K T LIR  ++  N  C+R +  +  LPC+CY N   N+TV  G  CQNG+ SRV+EL VEK+  C K  FKLRSI+C+  C+R  ++ VG C
Sbjct: 1486 GNTTKNINVVVGKQCQSGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVRLVKCQGCPKPSYKVEKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKC 1732          

HSP 6 Score: 102.064 bits (253), Expect = 7.011e-111
Identity = 63/222 (28.38%), Postives = 116/222 (52.25%), Query Frame = 3
Query:  747 NKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRT----SHRNCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTLNGKCQIKSLSIKGGKCGCLGS---TFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICP 1391
            N+R + VL+ ++  N  C WK+++S FV C   CP+P+ K + CI  ++ + R++++E G KC     +L  + CQ C + S Y + +C K +K ++LVRT    +++ C +K   +  + C C G     +NV     C NG   + L      N KC   +  ++  +C    S      ++ CV+  R++++    + G +C+ K+V S+ + C++ CP
Sbjct: 1735 NQRSLTVLRAVREKN-RCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGC-QNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCP 1954          

HSP 7 Score: 320.087 bits (819), Expect = 1.637e-110
Identity = 166/247 (67.21%), Postives = 193/247 (78.14%), Query Frame = 2
Query:    2 GNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQI-VGPC 739
            GN TKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKN CPLPKE+++RCI+G Q VM++TF  VGN C     RV + KCQ CP+  Y++++C+ N K T LIR  ++  N  C+R +  +  LPC+CY N   N+TV  G  CQNG+ SRV+EL VEK+  C K  FKLRSI+C   C+R  ++ VG C
Sbjct:  742 GNITKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPSYKVEKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCRKPCDRRRKVEVGKC 988          

HSP 8 Score: 100.138 bits (248), Expect = 1.637e-110
Identity = 63/222 (28.38%), Postives = 116/222 (52.25%), Query Frame = 3
Query:  747 NKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRT----SHRNCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTLNGKCQIKSLSIKGGKCGCLGS---TFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICP 1391
            N+R + VL+ ++  N  C WK+++S FV C   CP+P+ K + CI  +  + R++++E G KC     +L  + CQ C + S Y + +C K +K ++LVRT    +++ C +K   +  + C C G     +NV     C NG   + L      N KC   +  ++  +C    S      ++ CV+  R++++    + G++C+ K+V S+ + C++ CP
Sbjct:  991 NQRSLTVLRAVREKN-RCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKLAIFRVTFKEVGDKCRFSVIRLSVVPCQGC-QNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNITKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGNKCVLKVVNSKTVNCKNPCP 1210          

HSP 9 Score: 245.358 bits (625), Expect = 4.949e-83
Identity = 119/242 (49.17%), Postives = 172/242 (71.07%), Query Frame = 2
Query:    5 NTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQIV 730
            NT  N+ V  G+QCQNG+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E  +C+ K+V SK V CK  CP PKE+R+RCI   Q + ++TF  VG+KC    +R+++  CQ C  S YRI++C+K AKET L+RTF+Q  N  C++KE+ +K L C+C  N+T+NI V++G  CQNGY +RVLEL  E+N  C+K  FKLR I+C+  C+R  ++V
Sbjct:  557 NTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNITKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLV 798          

HSP 10 Score: 83.1889 bits (204), Expect = 4.949e-83
Identity = 57/222 (25.68%), Postives = 109/222 (49.10%), Query Frame = 3
Query:  747 NKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRTSHR----NCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTLNGKCQIKSLSIKGGKC--GC-LGSTFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICP 1391
            NKRL++ +K +K     C  K+++S  VNC + CP P+ K + CI G++ ++R++++  G  C     ++  +KCQ C + S Y++ +C    K + L+R ++R    +C R    +  + C C       + VT    C NG   + +      +GKC   +  ++  +C   C       +  C +  R L      +  ++C+ K+V S+ ++C+ +CP
Sbjct:  805 NKRLIQRMKSVKEGK-KCVLKVVNSKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPS-YKVEKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCRKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCP 1024          

HSP 11 Score: 242.276 bits (617), Expect = 6.619e-83
Identity = 118/242 (48.76%), Postives = 171/242 (70.66%), Query Frame = 2
Query:    5 NTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQIV 730
            NT  N+ V  G+QCQNG+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E  +C+ K+V SK V CK  CP PKE+R+RCI     + ++TF  VG+KC    +R+++  CQ C  S YRI++C+K AKET L+RTF+Q  N  C++KE+ +K L C+C  N+T+NI V++G  CQNGY +RVLEL  E+N  C+K  FKLR I+C+  C+R  ++V
Sbjct:  929 NTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCRKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKLAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNITKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLV 1170          

HSP 12 Score: 85.8853 bits (211), Expect = 6.619e-83
Identity = 59/222 (26.58%), Postives = 110/222 (49.55%), Query Frame = 3
Query:  747 NKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRTSHR----NCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTLNGKCQIKSLSIKGGKC--GC-LGSTFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICP 1391
            NKRL++ +K +K  N  C  K+++S  VNC + CP P+ + T CI G + +++I++   G KC     ++   KCQ C +K+ Y++ +C+   K + L+R ++R    +C R    +  + C C       + VT    C NG   + +      +GKC   +  ++  +C   C       +  C +  R L      +  ++C+ K+V S+ ++C+ +CP
Sbjct: 1177 NKRLIQRMKSVKEGN-KCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRC-QKTSYKVEKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCRKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCP 1396          

HSP 13 Score: 242.276 bits (617), Expect = 1.720e-82
Identity = 118/242 (48.76%), Postives = 171/242 (70.66%), Query Frame = 2
Query:    5 NTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQIV 730
            NT  N+ V  G+QCQNG+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E  +C+ K+V SK V CK  CP PKE+R+RCI   Q + ++TF  VG+KC    +R+++  CQ C  S YRI++C+K AKET L+RTF+Q  N  C++KE+ +K L C+C  N T+NI V++G  CQ+GY +RVLEL  E+N  C+K  FKLR I+C+  C+R  ++V
Sbjct: 1301 NTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCRKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQSGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLV 1542          

HSP 14 Score: 84.3445 bits (207), Expect = 1.720e-82
Identity = 56/222 (25.23%), Postives = 110/222 (49.55%), Query Frame = 3
Query:  747 NKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRTSHR----NCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTLNGKCQIKSLSIKGGKCG---CLGSTFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICP 1391
            NKRL++ +K +K     C  K+++S  VNC + CP P+ K + CI G++ ++R++++  G  C     +++ +KCQ C + S Y++ +C+   K + L+R ++R    +C R    +  + C C       + VT    C NG   + +      +GKC   +  ++  +C           +  C +  R L      +  ++C+ K+V S+ ++C+ +CP
Sbjct: 1549 NKRLIQRMKSVKEGK-KCVLKVVNSKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVRLVKCQGCPKPS-YKVEKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCP 1768          

HSP 15 Score: 244.202 bits (622), Expect = 6.075e-82
Identity = 119/242 (49.17%), Postives = 171/242 (70.66%), Query Frame = 2
Query:    5 NTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQIV 730
            NT  N+ V  G+QCQNG+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E  +C+ K+V SK V CK  CP PKE+R+RCI   Q + ++TF  VG+KC    +R+++  CQ C  S YRI++C+K AKET L+RTF+Q  N  C++KE+ +K L C+C  N T+NI V++G  CQNGY +RVLEL  E+N  C+K  FKLR I+C+  C+R  ++V
Sbjct: 1673 NTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLV 1914          

HSP 16 Score: 80.4925 bits (197), Expect = 6.075e-82
Identity = 64/222 (28.83%), Postives = 107/222 (48.20%), Query Frame = 3
Query:  747 NKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRTSHRN----CTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTLNGKCQIKSLSIKGGKC--GCLGSTFNLSVCVNGNRILEKFTRYQSGS-QCLSKLVFSRKIRCRSICP 1391
            NKRL++ +K +K     C  K+++S  VNC + CP P+ + T CI G + +++I++   G KC     ++   KCQ C R SVY +  C K +K + L+RT  ++    C RK  KL  + C C       + V    +C NG + + L      N  C  ++  ++  KC   C  +   +  C    R+++   + +S +  C  KL+ S  + C   CP
Sbjct: 1921 NKRLIQRMKSVKEGK-KCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPR-SVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQIVGPCFLNKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCP 2140          

HSP 17 Score: 139.813 bits (351), Expect = 3.197e-57
Identity = 96/291 (32.99%), Postives = 139/291 (47.77%), Query Frame = 2
Query:   20 INVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNS----------------------------------------------KTVNCK--NPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQ--RCPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQI-VGPC 739
            + VV GK+C+NGY  R+L   E    KC +    L  ++C   C+    LV + CV+ KR       V    KC LK+V +                                              K+ NC     CP PK   + CI+G + +MK+++ LVG KC  +  R+    C   +CP+  Y++++C  N K T LIR  ++  N  C+R +  +  LPC+CY N   N+TV  G  CQNG+ SRV+EL VEK+  C K  FKLRSI+C+  C+R  ++ VG C
Sbjct:  327 LKVVYGKECENGYKKRMLLYYEFSQFKCSETYLNLEPVKCDTLCN-GTTLVTSPCVNGKRNNTIYTKVFTNNKCSLKIVKTTESFCDTICPKSLIIESKECVNNIKDVFHITFQNVNGKCIKQTKKIKSNNCTKIQECPQPKFISSDCIEGKKILMKLSYRLVGGKCQEINERIGNENCSIAKCPKPSYKVEKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKC 616          

HSP 18 Score: 102.449 bits (254), Expect = 3.197e-57
Identity = 63/222 (28.38%), Postives = 117/222 (52.70%), Query Frame = 3
Query:  747 NKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRT----SHRNCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTLNGKCQIKSLSIKGGKCGCLGS---TFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICP 1391
            N+R + VL+ ++  N  C WK+++S FV C   CP+P+ K + CI  ++ + R++++E G KC     +L  + CQ C + S Y + +C K +K ++LVRT    +++ C +K   +  + C C G     +NV     C NG   + L      N KC   +  ++  +C    S      ++ CV+  R++++    + G +C+ K+V S+ + C+++CP
Sbjct:  619 NQRSLTVLRAVREKN-RCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGC-QNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNITKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNLCP 838          

HSP 19 Score: 131.339 bits (329), Expect = 3.356e-31
Identity = 83/234 (35.47%), Postives = 126/234 (53.85%), Query Frame = 2
Query:    2 GNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGK-KCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPR-SVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKC 697
             N T+NI V++G  CQNGY +RVLEL  E+N  C+K  FKLR I+C+  C+R    +V  C  NKRL++ +K +K     C  K+++S  VNC + CP P+ + T CI G + +++I++   G KC     ++   KCQ C R SVY +  C K +K + L+RT  ++    C RK  KL  + C C       + V    +C NG + + L      N  C  ++  ++  KC
Sbjct: 2044 DNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNR-TNQIVGPCFLNKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRTSHRN----CTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTVNGKCQVKSLSIKGGKC 2272          

HSP 20 Score: 70.4774 bits (171), Expect = 3.101e-12
Identity = 52/226 (23.01%), Postives = 105/226 (46.46%), Query Frame = 3
Query:  744 LNKRLVKVLKQIKSNNGSCHWKLLSSTFVNCS--HKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCF-RKSVYELMECSKTSKTSELVRTSHR----NCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTLNGKCQIKSLSIKGGKCG---CLGSTFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICP 1391
            +N + +K  K+IKSNN              C+   +CP+P+  ++ CI G++ ++++SY+  GGKC++  +++    C      K  Y++ +C+   K + L+R ++R    +C R    +  + C C       + VT    C NG   + +      +GKC   +  ++  +C           +  C +  R L      +  ++C+ K+V S+ ++C+ +CP
Sbjct:  441 VNGKCIKQTKKIKSNN--------------CTKIQECPQPKFISSDCIEGKKILMKLSYRLVGGKCQEINERIGNENCSIAKCPKPSYKVEKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCP 652          

HSP 21 Score: 64.6994 bits (156), Expect = 2.155e-10
Identity = 96/367 (26.16%), Postives = 151/367 (41.14%), Query Frame = 3
Query:  843 KCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKC------------QTCFRKSVYELMECSKTSKTSELVRTSHRNCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTLNGKCQIKSLSIKGGKCG--CLGSTFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICPTNSMVXXXXXXXXXXXXVISLFEKSYNQCKLVKFSFKWTKCIIPISGKNCSKIK-----RLTMSNCQKGKAYIIFTKSAEKPVNGTCGLNSSKIAQVNCT-DKCERTIRPKLVAGIC---EKQIQLVFFIASRPQGKQCLHKINYLGKIGCKNC*KEMIRNREQNLYQNF 1874
            KC  P +K   C+N E+  V  ++    GKC    +KL T+KC            +T    + Y  +     S+ ++L RT   N       L    C C       L V     C NG + + L +Y     KC    L+++  KC   C G+T   S CVNG R    +T+  + ++C  K+V + +  C +ICP + ++ S +C+   K      F+    +C       K TK    I   NC+KI+     +   S+C +GK   I  K + + V G C   + +I   NC+  KC    +P      C   +K   L+  +A R     C+     + K+ C     +  RN E N+   F
Sbjct:  228 KCSAPIVKKGPCVNKEQINVITTFYYYNGKCVSENRKL-TVKCLGPKDCPDPYYQKTSCGSANYSFILFHNFSRVNDLCRTETVN-------LKKYSCGCSKPEESVLKVVYGKECENGYKKRMLLYYEFSQFKCSETYLNLEPVKCDTLCNGTTLVTSPCVNGKRNNTIYTKVFTNNKCSLKIVKTTESFCDTICPKSLIIESKECVNNIKDVFHITFQNVNGKC------IKQTK---KIKSNNCTKIQECPQPKFISSDCIEGKK--ILMKLSYRLVGGKCQEINERIGNENCSIAKCP---KPSYKVEKCNNNQKNTSLI-RVAYRQTNSSCVRTQTTVTKLPC-----QCYRNTETNVTVTF 566          

HSP 22 Score: 56.9954 bits (136), Expect = 4.739e-8
Identity = 41/154 (26.62%), Postives = 66/154 (42.86%), Query Frame = 2
Query:  287 CPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKC---QRCPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQIVGPC 739
            C  P  ++  C+   Q  +  TF     KC +   ++ + KC   + CP   Y+   C  +A  +F++   F   N +C  + + LK   C C       + V+ G  C+NGYK R+L         C +    L  +KC+  CN T  +  PC
Sbjct:  229 CSAPIVKKGPCVNKEQINVITTFYYYNGKCVSENRKLTV-KCLGPKDCPDPYYQKTSCG-SANYSFILFHNFSRVNDLCRTETVNLKKYSCGCSKPEESVLKVVYGKECENGYKKRMLLYYEFSQFKCSETYLNLEPVKCDTLCNGTTLVTSPC 380          
BLAST of SMED30031362 vs. Planmine SMEST
Match: SMESG000033673.1 (SMESG000033673.1)

HSP 1 Score: 697.582 bits (1799), Expect = 0.000e+0
Identity = 359/368 (97.55%), Postives = 365/368 (99.18%), Query Frame = 3
Query:  723 KLLALVFLNKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRTSHRNCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTLNGKCQIKSLSIKGGKCGCLGSTFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICPTNSMVXXXXXXXXXXXXVISLFEKSYNQCKLVKFSFKWTKCIIPISGKNCSKIKRLTMSNCQKGKAYIIFTKSAEKPVNGTCGLNSSKIAQVNCTDKCERTIRPKLVAGICEKQIQLVFFIASRPQGKQCLHKINYLGKIGCKNC 1826
            +++   FLNKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRTSHRNCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTT+NGKCQ+KSLSIKGGKCGCLGSTFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICPTNS+VLSSKCLKKRKLRVISLFEKSYNQCKLVKFSFKWTKCIIPISGKNCSKIKRLTMSNCQKGKAYIIFTKSAEKPVNGTCGLNSSKIAQVNCTDKCERTIRPKLVAGICEKQIQLVFFIASRPQGKQCLHKINYLGKIGCKNC
Sbjct: 2047 QIVGPCFLNKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRTSHRNCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTVNGKCQVKSLSIKGGKCGCLGSTFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICPTNSVVLSSKCLKKRKLRVISLFEKSYNQCKLVKFSFKWTKCIIPISGKNCSKIKRLTMSNCQKGKAYIIFTKSAEKPVNGTCGLNSSKIAQVNCTDKCERTIRPKLVAGICEKQIQLVFFIASRPQGKQCLHKINYLGKIGCKNC 2414          

HSP 2 Score: 445.277 bits (1144), Expect = 0.000e+0
Identity = 231/248 (93.15%), Postives = 231/248 (93.15%), Query Frame = 2
Query:    2 GNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQIVGPCFL 745
            GNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVG                 CPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQIVGPCFL
Sbjct: 1824 GNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVG-----------------CPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQIVGPCFL 2054          

HSP 3 Score: 358.221 bits (918), Expect = 5.789e-114
Identity = 185/247 (74.90%), Postives = 205/247 (83.00%), Query Frame = 2
Query:    2 GNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQI-VGPC 739
            GN TKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEG KCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRC ++ Y++++C  N K T LIR  ++  N  C+R +  +  LPC+CY N   N+TV  G  CQNG+ SRV+EL VEK+  C K  FKLRSI+C   C+R  ++ VG C
Sbjct: 1114 GNITKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGNKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCQKTSYKVEKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCRKPCDRRRKVEVGKC 1360          

HSP 4 Score: 73.559 bits (179), Expect = 5.789e-114
Identity = 52/218 (23.85%), Postives = 96/218 (44.04%), Query Frame = 3
Query:  747 NKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRTSHRNCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTLNGKCQIKSLSIKGGKCGCLGS---TFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICP 1391
            N+R + VL+ ++  N  C WK+++S FV C   CP+P+ K + CI  ++ + R++++E G KC     +L  + CQ  F                                 LL +   C G     +NV     C +G   + L      N KC   +  ++  +C    S      ++ CV+  R++++    + G +C+ K+V S+ + C+++CP
Sbjct: 1363 NQRSLTVLRAVREKN-RCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQDSF-------------------------------NILLILSGRCTGNTTKNINVVVGKQCQSGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNLCP 1548          

HSP 5 Score: 318.931 bits (816), Expect = 8.923e-111
Identity = 166/247 (67.21%), Postives = 194/247 (78.54%), Query Frame = 2
Query:    2 GNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQI-VGPC 739
            GNTTKNINVVVGKQCQ+GYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKN CPLPKE+++RCI+G Q VM++TF  VGN C     RV + KCQ CP+  Y++++C  N K T LIR  ++  N  C+R +  +  LPC+CY N   N+TV  G  CQNG+ SRV+EL VEK+  C K  FKLRSI+C+  C+R  ++ VG C
Sbjct: 1452 GNTTKNINVVVGKQCQSGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVRLVKCQGCPKPSYKVEKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKC 1698          

HSP 6 Score: 102.064 bits (253), Expect = 8.923e-111
Identity = 63/222 (28.38%), Postives = 116/222 (52.25%), Query Frame = 3
Query:  747 NKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRT----SHRNCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTLNGKCQIKSLSIKGGKCGCLGS---TFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICP 1391
            N+R + VL+ ++  N  C WK+++S FV C   CP+P+ K + CI  ++ + R++++E G KC     +L  + CQ C + S Y + +C K +K ++LVRT    +++ C +K   +  + C C G     +NV     C NG   + L      N KC   +  ++  +C    S      ++ CV+  R++++    + G +C+ K+V S+ + C++ CP
Sbjct: 1701 NQRSLTVLRAVREKN-RCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGC-QNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCP 1920          

HSP 7 Score: 320.087 bits (819), Expect = 2.066e-110
Identity = 166/247 (67.21%), Postives = 193/247 (78.14%), Query Frame = 2
Query:    2 GNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQI-VGPC 739
            GN TKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKN CPLPKE+++RCI+G Q VM++TF  VGN C     RV + KCQ CP+  Y++++C+ N K T LIR  ++  N  C+R +  +  LPC+CY N   N+TV  G  CQNG+ SRV+EL VEK+  C K  FKLRSI+C   C+R  ++ VG C
Sbjct:  742 GNITKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPSYKVEKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCRKPCDRRRKVEVGKC 988          

HSP 8 Score: 100.138 bits (248), Expect = 2.066e-110
Identity = 63/222 (28.38%), Postives = 116/222 (52.25%), Query Frame = 3
Query:  747 NKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRT----SHRNCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTLNGKCQIKSLSIKGGKCGCLGS---TFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICP 1391
            N+R + VL+ ++  N  C WK+++S FV C   CP+P+ K + CI  +  + R++++E G KC     +L  + CQ C + S Y + +C K +K ++LVRT    +++ C +K   +  + C C G     +NV     C NG   + L      N KC   +  ++  +C    S      ++ CV+  R++++    + G++C+ K+V S+ + C++ CP
Sbjct:  991 NQRSLTVLRAVREKN-RCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKLAIFRVTFKEVGDKCRFSVIRLSVVPCQGC-QNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNITKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGNKCVLKVVNSKTVNCKNPCP 1210          

HSP 9 Score: 242.276 bits (617), Expect = 4.986e-86
Identity = 118/242 (48.76%), Postives = 171/242 (70.66%), Query Frame = 2
Query:    5 NTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQIV 730
            NT  N+ V  G+QCQNG+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E  +C+ K+V SK V CK  CP PKE+R+RCI     + ++TF  VG+KC    +R+++  CQ C  S YRI++C+K AKET L+RTF+Q  N  C++KE+ +K L C+C  N+T+NI V++G  CQNGY +RVLEL  E+N  C+K  FKLR I+C+  C+R  ++V
Sbjct:  929 NTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCRKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKLAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNITKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLV 1170          

HSP 10 Score: 96.2857 bits (238), Expect = 4.986e-86
Identity = 95/376 (25.27%), Postives = 175/376 (46.54%), Query Frame = 3
Query:  747 NKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRTSHR----NCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTLNGKCQIKSLSIKGGKC--GC-LGSTFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICPTNSMVXXXXXXXXXXXXVISLFEKSYNQCKLVKFSFKWTKC------IIPISGK---NCSK-IKRLTMSNCQKGKAYIIFTKSAEKPVNGTCGLNSSKIAQVNCTDKCERTIRPKLVAGICEKQIQLVFFIASRPQGKQCLHKINYLGKIGCKN 1823
            NKRL++ +K +K  N  C  K+++S  VNC + CP P+ + T CI G + +++I++   G KC     ++   KCQ C +K+ Y++ +C+   K + L+R ++R    +C R    +  + C C       + VT    C NG   + +      +GKC   +  ++  +C   C       +  C +  R L      +  ++C+ K+V S+ ++C+ +CP      S   LKK+ +  ++ F++  ++C+          C      ++ +SG+   N +K I  +    CQ G  YI       +  N  C   + K+ ++ C   C R +  KLV   C    +L+  + S  +GK+C+ K+     + CKN
Sbjct: 1177 NKRLIQRMKSVKEGN-KCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRC-QKTSYKVEKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCRKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVT-FKEVGDKCRFSVIRLSVVPCQDSFNILLILSGRCTGNTTKNINVVVGKQCQSG--YITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKL--KLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKN 1545          

HSP 11 Score: 245.358 bits (625), Expect = 5.560e-83
Identity = 119/242 (49.17%), Postives = 172/242 (71.07%), Query Frame = 2
Query:    5 NTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQIV 730
            NT  N+ V  G+QCQNG+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E  +C+ K+V SK V CK  CP PKE+R+RCI   Q + ++TF  VG+KC    +R+++  CQ C  S YRI++C+K AKET L+RTF+Q  N  C++KE+ +K L C+C  N+T+NI V++G  CQNGY +RVLEL  E+N  C+K  FKLR I+C+  C+R  ++V
Sbjct:  557 NTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNITKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLV 798          

HSP 12 Score: 83.1889 bits (204), Expect = 5.560e-83
Identity = 57/222 (25.68%), Postives = 109/222 (49.10%), Query Frame = 3
Query:  747 NKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRTSHR----NCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTLNGKCQIKSLSIKGGKC--GC-LGSTFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICP 1391
            NKRL++ +K +K     C  K+++S  VNC + CP P+ K + CI G++ ++R++++  G  C     ++  +KCQ C + S Y++ +C    K + L+R ++R    +C R    +  + C C       + VT    C NG   + +      +GKC   +  ++  +C   C       +  C +  R L      +  ++C+ K+V S+ ++C+ +CP
Sbjct:  805 NKRLIQRMKSVKEGK-KCVLKVVNSKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVGLVKCQGCPKPS-YKVEKCDNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCRKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCP 1024          

HSP 13 Score: 244.202 bits (622), Expect = 6.775e-77
Identity = 119/242 (49.17%), Postives = 171/242 (70.66%), Query Frame = 2
Query:    5 NTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQIV 730
            NT  N+ V  G+QCQNG+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E  +C+ K+V SK V CK  CP PKE+R+RCI   Q + ++TF  VG+KC    +R+++  CQ C  S YRI++C+K AKET L+RTF+Q  N  C++KE+ +K L C+C  N T+NI V++G  CQNGY +RVLEL  E+N  C+K  FKLR I+C+  C+R  ++V
Sbjct: 1639 NTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGCQNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLV 1880          

HSP 14 Score: 63.929 bits (154), Expect = 6.775e-77
Identity = 59/222 (26.58%), Postives = 100/222 (45.05%), Query Frame = 3
Query:  747 NKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRTSHRN----CTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTLNGKCQIKSLSIKGGKC--GCLGSTFNLSVCVNGNRILEKFTRYQSGS-QCLSKLVFSRKIRCRSICP 1391
            NKRL++ +K +K     C  K+++S  VNC + CP P+ + T CI G + +++I++   G                 C R SVY +  C K +K + L+RT  ++    C RK  KL  + C C       + V    +C NG + + L      N  C  ++  ++  KC   C  +   +  C    R+++   + +S +  C  KL+ S  + C   CP
Sbjct: 1887 NKRLIQRMKSVKEGK-KCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVG-----------------CPR-SVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQIVGPCFLNKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCP 2089          

HSP 15 Score: 179.104 bits (453), Expect = 1.526e-63
Identity = 99/242 (40.91%), Postives = 145/242 (59.92%), Query Frame = 2
Query:    5 NTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQIV 730
            NT  N+ V  G+QCQNG+++RV+EL  E++ KC KVAFKLR IRC++PC R+ K+ V KC SN+R +  +++V+E  +C+ K+V SK V CK  CP PKE+R+RCI   Q + ++TF  VG+KC    +R+++  CQ                 ++F I                 L  L  +C  N T+NI V++G  CQ+GY +RVLEL  E+N  C+K  FKLR I+C+  C+R  ++V
Sbjct: 1301 NTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCRKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQ-----------------DSFNI-----------------LLILSGRCTGNTTKNINVVVGKQCQSGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLV 1508          

HSP 16 Score: 84.3445 bits (207), Expect = 1.526e-63
Identity = 56/222 (25.23%), Postives = 110/222 (49.55%), Query Frame = 3
Query:  747 NKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRTSHR----NCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTLNGKCQIKSLSIKGGKCG---CLGSTFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICP 1391
            NKRL++ +K +K     C  K+++S  VNC + CP P+ K + CI G++ ++R++++  G  C     +++ +KCQ C + S Y++ +C+   K + L+R ++R    +C R    +  + C C       + VT    C NG   + +      +GKC   +  ++  +C           +  C +  R L      +  ++C+ K+V S+ ++C+ +CP
Sbjct: 1515 NKRLIQRMKSVKEGK-KCVLKVVNSKTVNCKNLCPLPKEKKSRCIRGKQAVMRLTFRNVGNVCLYSTARVRLVKCQGCPKPS-YKVEKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCP 1734          

HSP 17 Score: 139.813 bits (351), Expect = 3.445e-57
Identity = 96/291 (32.99%), Postives = 139/291 (47.77%), Query Frame = 2
Query:   20 INVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNS----------------------------------------------KTVNCK--NPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQ--RCPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQI-VGPC 739
            + VV GK+C+NGY  R+L   E    KC +    L  ++C   C+    LV + CV+ KR       V    KC LK+V +                                              K+ NC     CP PK   + CI+G + +MK+++ LVG KC  +  R+    C   +CP+  Y++++C  N K T LIR  ++  N  C+R +  +  LPC+CY N   N+TV  G  CQNG+ SRV+EL VEK+  C K  FKLRSI+C+  C+R  ++ VG C
Sbjct:  327 LKVVYGKECENGYKKRMLLYYEFSQFKCSETYLNLEPVKCDTLCN-GTTLVTSPCVNGKRNNTIYTKVFTNNKCSLKIVKTTESFCDTICPKSLIIESKECVNNIKDVFHITFQNVNGKCIKQTKKIKSNNCTKIQECPQPKFISSDCIEGKKILMKLSYRLVGGKCQEINERIGNENCSIAKCPKPSYKVEKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKC 616          

HSP 18 Score: 102.064 bits (253), Expect = 3.445e-57
Identity = 63/222 (28.38%), Postives = 117/222 (52.70%), Query Frame = 3
Query:  747 NKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRT----SHRNCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTLNGKCQIKSLSIKGGKCGCLGS---TFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICP 1391
            N+R + VL+ ++  N  C WK+++S FV C   CP+P+ K + CI  ++ + R++++E G KC     +L  + CQ C + S Y + +C K +K ++LVRT    +++ C +K   +  + C C G     +NV     C NG   + L      N KC   +  ++  +C    S      ++ CV+  R++++    + G +C+ K+V S+ + C+++CP
Sbjct:  619 NQRSLTVLRAVREKN-RCMWKIVTSKFVKCKKVCPDPKEKRSRCILKKQAIFRVTFKEVGDKCRFSVIRLSVVPCQGC-QNSTYRIEKCDKGAKETKLVRTFYQETNQTCVKKEVIVKKLSCRCTGNITKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNLCP 838          

HSP 19 Score: 131.339 bits (329), Expect = 3.083e-31
Identity = 83/234 (35.47%), Postives = 126/234 (53.85%), Query Frame = 2
Query:    2 GNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPCSRKLKLVVNKCVSNKRLIQRMKSVKEGK-KCVLKVVNSKTVNCKNPCPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPR-SVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKC 697
             N T+NI V++G  CQNGY +RVLEL  E+N  C+K  FKLR I+C+  C+R    +V  C  NKRL++ +K +K     C  K+++S  VNC + CP P+ + T CI G + +++I++   G KC     ++   KCQ C R SVY +  C K +K + L+RT  ++    C RK  KL  + C C       + V    +C NG + + L      N  C  ++  ++  KC
Sbjct: 1993 DNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNR-TNQIVGPCFLNKRLVKVLKQIKSNNGSCHWKLLSSTFVNCSHKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVRTSHRN----CTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTVNGKCQVKSLSIKGGKC 2221          

HSP 20 Score: 70.4774 bits (171), Expect = 3.284e-12
Identity = 52/226 (23.01%), Postives = 105/226 (46.46%), Query Frame = 3
Query:  744 LNKRLVKVLKQIKSNNGSCHWKLLSSTFVNCS--HKCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKCQTCF-RKSVYELMECSKTSKTSELVRTSHR----NCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTLNGKCQIKSLSIKGGKCG---CLGSTFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICP 1391
            +N + +K  K+IKSNN              C+   +CP+P+  ++ CI G++ ++++SY+  GGKC++  +++    C      K  Y++ +C+   K + L+R ++R    +C R    +  + C C       + VT    C NG   + +      +GKC   +  ++  +C           +  C +  R L      +  ++C+ K+V S+ ++C+ +CP
Sbjct:  441 VNGKCIKQTKKIKSNN--------------CTKIQECPQPKFISSDCIEGKKILMKLSYRLVGGKCQEINERIGNENCSIAKCPKPSYKVEKCNNNQKNTSLIRVAYRQTNSSCVRTQTTVTKLPCQCYRNTETNVTVTFGRQCQNGFLSRVIELNVEKSGKCSKVAFKLRSIRCKKPCDRRRKVEVGKCKSNQRSLTVLRAVREKNRCMWKIVTSKFVKCKKVCP 652          

HSP 21 Score: 64.3142 bits (155), Expect = 2.402e-10
Identity = 96/367 (26.16%), Postives = 151/367 (41.14%), Query Frame = 3
Query:  843 KCPEPQIKTTACINGERFMVRISYQETGGKCEKREQKLQTLKC------------QTCFRKSVYELMECSKTSKTSELVRTSHRNCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTTLNGKCQIKSLSIKGGKCG--CLGSTFNLSVCVNGNRILEKFTRYQSGSQCLSKLVFSRKIRCRSICPTNSMVXXXXXXXXXXXXVISLFEKSYNQCKLVKFSFKWTKCIIPISGKNCSKIK-----RLTMSNCQKGKAYIIFTKSAEKPVNGTCGLNSSKIAQVNCT-DKCERTIRPKLVAGIC---EKQIQLVFFIASRPQGKQCLHKINYLGKIGCKNC*KEMIRNREQNLYQNF 1874
            KC  P +K   C+N E+  V  ++    GKC    +KL T+KC            +T    + Y  +     S+ ++L RT   N       L    C C       L V     C NG + + L +Y     KC    L+++  KC   C G+T   S CVNG R    +T+  + ++C  K+V + +  C +ICP + ++ S +C+   K      F+    +C       K TK    I   NC+KI+     +   S+C +GK   I  K + + V G C   + +I   NC+  KC    +P      C   +K   L+  +A R     C+     + K+ C     +  RN E N+   F
Sbjct:  228 KCSAPIVKKGPCVNKEQINVITTFYYYNGKCVSENRKL-TVKCLGPKDCPDPYYQKTSCGSANYSFILFHNFSRVNDLCRTETVN-------LKKYSCGCSKPEESVLKVVYGKECENGYKKRMLLYYEFSQFKCSETYLNLEPVKCDTLCNGTTLVTSPCVNGKRNNTIYTKVFTNNKCSLKIVKTTESFCDTICPKSLIIESKECVNNIKDVFHITFQNVNGKC------IKQTK---KIKSNNCTKIQECPQPKFISSDCIEGKK--ILMKLSYRLVGGKCQEINERIGNENCSIAKCP---KPSYKVEKCNNNQKNTSLI-RVAYRQTNSSCVRTQTTVTKLPC-----QCYRNTETNVTVTF 566          

HSP 22 Score: 56.9954 bits (136), Expect = 4.380e-8
Identity = 41/154 (26.62%), Postives = 66/154 (42.86%), Query Frame = 2
Query:  287 CPLPKERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKC---QRCPRSVYRIDRCEKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPSCQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQIVGPC 739
            C  P  ++  C+   Q  +  TF     KC +   ++ + KC   + CP   Y+   C  +A  +F++   F   N +C  + + LK   C C       + V+ G  C+NGYK R+L         C +    L  +KC+  CN T  +  PC
Sbjct:  229 CSAPIVKKGPCVNKEQINVITTFYYYNGKCVSENRKLTV-KCLGPKDCPDPYYQKTSCG-SANYSFILFHNFSRVNDLCRTETVNLKKYSCGCSKPEESVLKVVYGKECENGYKKRMLLYYEFSQFKCSETYLNLEPVKCDTLCNGTTLVTSPC 380          
The following BLAST results are available for this feature:
BLAST of SMED30031362 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031362 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031362 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031362 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031362 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031362 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031362 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031362 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031362 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031362 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031362 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031362 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031362 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031362 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000033673.11.891e-12297.55SMESG000033673.1[more]
SMESG000033673.15.789e-11497.55SMESG000033673.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031362 ID=SMED30031362|Name=SMED30031362|organism=Schmidtea mediterranea sexual|type=transcript|length=1929bp
CGGAAACACTACAAAAAACATTAACGTGGTCGTTGGGAAACAATGTCAAA
ACGGATACATTACGAGAGTTTTAGAATTGACTGAAGAAAGAAACGACAAA
TGTGTAAAAGTCGCTTTCAAGTTGAGAAGAATCAGATGTCAAAGACCATG
CAGTAGGAAACTGAAGCTCGTCGTCAATAAATGCGTATCGAATAAGAGAT
TGATTCAACGCATGAAATCCGTGAAAGAAGGAAAGAAATGCGTATTGAAG
GTCGTCAACAGCAAGACTGTGAATTGTAAGAATCCTTGTCCATTACCGAA
AGAAAGGCGTACCAGGTGTATAAAAGGAATTCAAGGAGTGATGAAAATTA
CTTTTAATTTAGTTGGTAATAAATGTAATGCATTATTCGTTCGAGTCAAC
ATTACAAAATGTCAAAGATGTCCTAGAAGTGTTTACCGAATTGATAGATG
TGAAAAGAACGCCAAGGAAACATTTCTAATTCGAACGTTTTTCCAGTCAG
CAAATGGTGTTTGCCTTAGAAAAGAACTAAAATTGAAAACCTTACCATGT
AAATGCTATGACAATGTCACCAGAAACATCACCGTCATTATTGGTCCATC
ATGCCAAAACGGATACAAATCAAGAGTGTTGGAATTGAGAGTGGAAAAGA
ACCAAAATTGTATCAAAGAAACCTTCAAATTACGAAGCATCAAATGCGAA
GGTGCTTGCAATAGAACTAATCAAATTGTTGGCCCTTGTTTTCTTAAATA
AGCGTCTCGTAAAAGTTCTGAAACAAATTAAAAGCAACAATGGCTCATGT
CATTGGAAATTGTTGAGTTCTACATTTGTCAATTGTTCTCACAAATGCCC
AGAACCTCAAATTAAAACCACCGCTTGCATCAATGGGGAAAGATTTATGG
TTAGAATATCATACCAAGAAACCGGAGGGAAATGTGAAAAGCGCGAACAA
AAATTACAGACATTAAAATGTCAAACATGCTTCCGAAAAAGTGTATATGA
ACTTATGGAATGTTCTAAGACATCAAAAACGTCAGAGTTGGTTAGAACAT
CCCATAGAAATTGTACGAGAAAAGTCGAAAAACTTCTGGATGTTGATTGT
TTATGCGAAGGATTAAACAATTACGGTTTAAATGTAACATCAACTGCGAA
TTGTACAAATGGCGTTAGGGTCAAAACCCTTTCATTTTATACAACACTAA
ATGGAAAATGTCAAATAAAATCCCTATCAATAAAAGGGGGGAAATGTGGC
TGCTTGGGCTCAACCTTCAATCTATCAGTTTGTGTTAACGGAAACCGAAT
ATTGGAGAAGTTTACCCGATACCAATCTGGATCCCAGTGTTTGTCAAAGT
TGGTTTTCAGTAGAAAAATACGTTGTAGATCAATCTGTCCTACAAATTCA
ATGGTTTTGTCAAGTAAATGTCTCAAAAAAAGAAAACTACGAGTGATTAG
TCTATTTGAAAAATCCTACAATCAATGCAAATTAGTAAAGTTTAGTTTCA
AGTGGACAAAATGTATCATCCCAATATCTGGGAAAAATTGCTCTAAAATC
AAGAGACTGACGATGTCTAATTGTCAAAAAGGAAAAGCTTACATAATTTT
TACAAAATCTGCTGAAAAACCCGTTAATGGAACTTGTGGACTGAACTCCT
CAAAAATAGCCCAAGTGAATTGCACAGATAAATGCGAGAGAACTATTCGA
CCGAAATTAGTTGCTGGAATTTGTGAAAAACAAATTCAACTCGTATTTTT
CATAGCATCAAGACCACAAGGAAAACAGTGCCTGCATAAAATCAACTATT
TAGGAAAGATCGGTTGTAAAAATTGCTAAAAAGAAATGATTAGGAACCGA
GAACAGAATTTATATCAGAATTTTGTTAACTAAATTATACGACTGTTTAA
TTTTCAAATATATATATATATAATTGCTA
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protein sequence of SMED30031362-orf-1

>SMED30031362-orf-1 ID=SMED30031362-orf-1|Name=SMED30031362-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=311bp
MVRISYQETGGKCEKREQKLQTLKCQTCFRKSVYELMECSKTSKTSELVR
TSHRNCTRKVEKLLDVDCLCEGLNNYGLNVTSTANCTNGVRVKTLSFYTT
LNGKCQIKSLSIKGGKCGCLGSTFNLSVCVNGNRILEKFTRYQSGSQCLS
KLVFSRKIRCRSICPTNSMVLSSKCLKKRKLRVISLFEKSYNQCKLVKFS
FKWTKCIIPISGKNCSKIKRLTMSNCQKGKAYIIFTKSAEKPVNGTCGLN
SSKIAQVNCTDKCERTIRPKLVAGICEKQIQLVFFIASRPQGKQCLHKIN
YLGKIGCKNC*
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protein sequence of SMED30031362-orf-2

>SMED30031362-orf-2 ID=SMED30031362-orf-2|Name=SMED30031362-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=250bp
GNTTKNINVVVGKQCQNGYITRVLELTEERNDKCVKVAFKLRRIRCQRPC
SRKLKLVVNKCVSNKRLIQRMKSVKEGKKCVLKVVNSKTVNCKNPCPLPK
ERRTRCIKGIQGVMKITFNLVGNKCNALFVRVNITKCQRCPRSVYRIDRC
EKNAKETFLIRTFFQSANGVCLRKELKLKTLPCKCYDNVTRNITVIIGPS
CQNGYKSRVLELRVEKNQNCIKETFKLRSIKCEGACNRTNQIVGPCFLK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000110pharynx progenitor cell
PLANA:0000418head
PLANA:0000420parapharyngeal region