ATP synthase-coupling factor 6, mitochondrial
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30031354 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Ensembl Fly
Match: ATPsynCF6 (gene:FBgn0016119 transcript:FBtr0333777) HSP 1 Score: 48.9062 bits (115), Expect = 5.057e-8 Identity = 32/96 (33.33%), Postives = 52/96 (54.17%), Query Frame = 3 Query: 66 LSISKLNVIRCFATGSR----------NPSSDPIQNLFLKRIREFREKSSKAEFGLVGASAVEIKTFQDSMDRIDAVYHAKG-ADMESFPQFSFKD 320 LS S L+ +R T +R N +SDPIQ LFL ++RE+++KS+ + LV ++ + + +DR+ + + G DM FP+F F D Sbjct: 2 LSQSLLSGMRVLRTEARRNFGIVAPALNKASDPIQQLFLDKVREYKQKSAGGK--LVDSNPDIERELKTELDRVAKQFGSDGKTDMLKFPEFQFPD 95
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Ensembl Fly
Match: ATPsynCF6 (gene:FBgn0016119 transcript:FBtr0084432) HSP 1 Score: 48.9062 bits (115), Expect = 5.057e-8 Identity = 32/96 (33.33%), Postives = 52/96 (54.17%), Query Frame = 3 Query: 66 LSISKLNVIRCFATGSR----------NPSSDPIQNLFLKRIREFREKSSKAEFGLVGASAVEIKTFQDSMDRIDAVYHAKG-ADMESFPQFSFKD 320 LS S L+ +R T +R N +SDPIQ LFL ++RE+++KS+ + LV ++ + + +DR+ + + G DM FP+F F D Sbjct: 2 LSQSLLSGMRVLRTEARRNFGIVAPALNKASDPIQQLFLDKVREYKQKSAGGK--LVDSNPDIERELKTELDRVAKQFGSDGKTDMLKFPEFQFPD 95
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Ensembl Fly
Match: ATPsynCF6 (gene:FBgn0016119 transcript:FBtr0305982) HSP 1 Score: 48.9062 bits (115), Expect = 5.057e-8 Identity = 32/96 (33.33%), Postives = 52/96 (54.17%), Query Frame = 3 Query: 66 LSISKLNVIRCFATGSR----------NPSSDPIQNLFLKRIREFREKSSKAEFGLVGASAVEIKTFQDSMDRIDAVYHAKG-ADMESFPQFSFKD 320 LS S L+ +R T +R N +SDPIQ LFL ++RE+++KS+ + LV ++ + + +DR+ + + G DM FP+F F D Sbjct: 2 LSQSLLSGMRVLRTEARRNFGIVAPALNKASDPIQQLFLDKVREYKQKSAGGK--LVDSNPDIERELKTELDRVAKQFGSDGKTDMLKFPEFQFPD 95
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Ensembl Zebrafish
Match: atp5pf (ATP synthase peripheral stalk subunit F6 [Source:ZFIN;Acc:ZDB-GENE-040426-2534]) HSP 1 Score: 52.373 bits (124), Expect = 3.213e-9 Identity = 29/73 (39.73%), Postives = 41/73 (56.16%), Query Frame = 3 Query: 129 DPIQNLFLKRIREFREKSSKAEFGLVGASAVEIKTFQDSMDRIDAVYHAKGADMESFPQFSFKDENLEDPRPK 347 DPIQ LFL +IR++ K SKA G+V A V K + ++ +Y G D+ FPQFSF + L++ K Sbjct: 40 DPIQKLFLDKIRDYNSK-SKASGGVVDAGPVYQKNLAEETTKLQRLYG--GGDLSKFPQFSFTEPKLDETTTK 109
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Ensembl Zebrafish
Match: atp5pf (ATP synthase peripheral stalk subunit F6 [Source:ZFIN;Acc:ZDB-GENE-040426-2534]) HSP 1 Score: 52.373 bits (124), Expect = 3.213e-9 Identity = 29/73 (39.73%), Postives = 41/73 (56.16%), Query Frame = 3 Query: 129 DPIQNLFLKRIREFREKSSKAEFGLVGASAVEIKTFQDSMDRIDAVYHAKGADMESFPQFSFKDENLEDPRPK 347 DPIQ LFL +IR++ K SKA G+V A V K + ++ +Y G D+ FPQFSF + L++ K Sbjct: 40 DPIQKLFLDKIRDYNSK-SKASGGVVDAGPVYQKNLAEETTKLQRLYG--GGDLSKFPQFSFTEPKLDETTTK 109
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Ensembl Zebrafish
Match: atp5pf (ATP synthase peripheral stalk subunit F6 [Source:ZFIN;Acc:ZDB-GENE-040426-2534]) HSP 1 Score: 52.373 bits (124), Expect = 3.213e-9 Identity = 29/73 (39.73%), Postives = 41/73 (56.16%), Query Frame = 3 Query: 129 DPIQNLFLKRIREFREKSSKAEFGLVGASAVEIKTFQDSMDRIDAVYHAKGADMESFPQFSFKDENLEDPRPK 347 DPIQ LFL +IR++ K SKA G+V A V K + ++ +Y G D+ FPQFSF + L++ K Sbjct: 40 DPIQKLFLDKIRDYNSK-SKASGGVVDAGPVYQKNLAEETTKLQRLYG--GGDLSKFPQFSFTEPKLDETTTK 109
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Ensembl Zebrafish
Match: atp5pf (ATP synthase peripheral stalk subunit F6 [Source:ZFIN;Acc:ZDB-GENE-040426-2534]) HSP 1 Score: 49.6766 bits (117), Expect = 3.929e-8 Identity = 27/62 (43.55%), Postives = 36/62 (58.06%), Query Frame = 3 Query: 129 DPIQNLFLKRIREFREKSSKAEFGLVGASAVEIKTFQDSMDRIDAVYHAKGADMESFPQFSF 314 DPIQ LFL +IR++ K SKA G+V A V K + ++ +Y G D+ FPQFSF Sbjct: 40 DPIQKLFLDKIRDYNSK-SKASGGVVDAGPVYQKNLAEETTKLQRLYG--GGDLSKFPQFSF 98
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Ensembl Zebrafish
Match: si:dkey-22n8.3 (si:dkey-22n8.3 [Source:ZFIN;Acc:ZDB-GENE-110914-188]) HSP 1 Score: 46.595 bits (109), Expect = 2.487e-7 Identity = 27/69 (39.13%), Postives = 40/69 (57.97%), Query Frame = 3 Query: 129 DPIQNLFLKRIREFREKSSKAEFGLVGASAVEIKTFQDSMDRIDAVYHAKGADMESFPQFSFKDENLED 335 DPIQ LFL +IRE+ K S+A G V A A K F + + ++ +Y G D+ +FP F F + L++ Sbjct: 8 DPIQRLFLHKIREYSTK-SQASAGPVDAGADYEKAFSEELSKLQRLY--GGGDLTNFPGFKFSEPVLDE 73
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. UniProt/SwissProt
Match: sp|Q24407|ATP5J_DROME (ATP synthase-coupling factor 6, mitochondrial OS=Drosophila melanogaster OX=7227 GN=ATPsynCF6 PE=3 SV=1) HSP 1 Score: 48.9062 bits (115), Expect = 5.070e-7 Identity = 32/96 (33.33%), Postives = 52/96 (54.17%), Query Frame = 3 Query: 66 LSISKLNVIRCFATGSR----------NPSSDPIQNLFLKRIREFREKSSKAEFGLVGASAVEIKTFQDSMDRIDAVYHAKG-ADMESFPQFSFKD 320 LS S L+ +R T +R N +SDPIQ LFL ++RE+++KS+ + LV ++ + + +DR+ + + G DM FP+F F D Sbjct: 2 LSQSLLSGMRVLRTEARRNFGIVAPALNKASDPIQQLFLDKVREYKQKSAGGK--LVDSNPDIERELKTELDRVAKQFGSDGKTDMLKFPEFQFPD 95
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. TrEMBL
Match: A0A0R3T742 (Uncharacterized protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS2879 PE=4 SV=1) HSP 1 Score: 81.6481 bits (200), Expect = 8.371e-17 Identity = 46/123 (37.40%), Postives = 72/123 (58.54%), Query Frame = 3 Query: 66 LSISKLNVIRCF-----ATGSRNPSSDPIQNLFLKRIREFREKSSKAEFGLVGASAVEIKTFQDSMDRIDAVYHAKGADMESFPQFSFKDENLEDPRPKAKLTIPVCYQLE--ENNKVIDNLL 413 L + +L +R F AT + + DPIQ F++++RE++ KS AE GLVGAS +IK +DS+ ++D V++AKGADM FP K+ L +P + P LE E ++ +D++L Sbjct: 4 LVLGRLFYVRSFSVFQRATFATQQAVDPIQRAFVEKLREYQMKSKTAEMGLVGASETQIKELKDSLGKLDNVFNAKGADMTQFPILQHKEPELTNPGSSIVVEYPEPVLLEDVEKSERLDDVL 126
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. TrEMBL
Match: A0A0R3W6S9 (Uncharacterized protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS5924 PE=4 SV=1) HSP 1 Score: 81.2629 bits (199), Expect = 1.072e-16 Identity = 36/79 (45.57%), Postives = 55/79 (69.62%), Query Frame = 3 Query: 102 ATGSRNPSSDPIQNLFLKRIREFREKSSKAEFGLVGASAVEIKTFQDSMDRIDAVYHAKGADMESFPQFSFKDENLEDP 338 AT + P +DPIQ F++++RE++ KS AE GLVGAS +IK+ +DS++++D V++AKG DM FP K+ L +P Sbjct: 21 ATFATQPVADPIQRAFVEKLREYQGKSRTAEMGLVGASEAQIKSLKDSLEKLDNVFNAKGVDMTQFPVLQHKEPELANP 99
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. TrEMBL
Match: A0A0R3SFF0 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS3560 PE=4 SV=1) HSP 1 Score: 80.8777 bits (198), Expect = 1.449e-16 Identity = 38/96 (39.58%), Postives = 60/96 (62.50%), Query Frame = 3 Query: 66 LSISKLNVIRCFATGSR-----NPSSDPIQNLFLKRIREFREKSSKAEFGLVGASAVEIKTFQDSMDRIDAVYHAKGADMESFPQFSFKDENLEDP 338 L +L +R F+T R + DPIQ F++++RE++EKS AE GLVGA+ ++K +DS++++D V++AKG DM FP K+ L +P Sbjct: 4 LVCGRLFAVRAFSTVQRATFATQQAVDPIQRAFVEKLREYQEKSRTAEMGLVGATETQVKNLKDSLEKLDNVFNAKGVDMTQFPVLQHKEPELINP 99
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. TrEMBL
Match: A0A0R3WJ50 (Uncharacterized protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS679 PE=4 SV=1) HSP 1 Score: 80.4925 bits (197), Expect = 2.135e-16 Identity = 38/98 (38.78%), Postives = 63/98 (64.29%), Query Frame = 3 Query: 126 SDPIQNLFLKRIREFREKSSKAEFGLVGASAVEIKTFQDSMDRIDAVYHAKGADMESFPQFSFKDENLEDPRPKAKLTIPVCYQLEENNKV--IDNLL 413 +DPIQ F++++RE++ KS AE GLVGAS +IK+ +DS++++D V++AKG DM FP +K+ L +P + P L++ K+ +D +L Sbjct: 29 ADPIQRAFVEKLREYQGKSKTAEMGLVGASQAQIKSLKDSLEKLDNVFNAKGVDMTQFPVLQYKEPELVNPGSSIVVEYPPAEPLQDVEKIERMDGIL 126
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. TrEMBL
Match: U6JIX2 (ATPase F0 complex subunit F6 mitochondrial OS=Echinococcus granulosus OX=6210 GN=EGR_08199 PE=4 SV=1) HSP 1 Score: 79.7221 bits (195), Expect = 4.938e-16 Identity = 40/96 (41.67%), Postives = 60/96 (62.50%), Query Frame = 3 Query: 66 LSISKLNVIRCF-----ATGSRNPSSDPIQNLFLKRIREFREKSSKAEFGLVGASAVEIKTFQDSMDRIDAVYHAKGADMESFPQFSFKDENLEDP 338 L S+L R F AT + P +DPIQ F++++RE++ KS AE GLV AS +IK+ +DS++++D V++AKG DM FP K+ L +P Sbjct: 4 LRCSRLLASRAFSITQRATFATQPVADPIQRAFVEKLREYQGKSKTAEMGLVEASQAQIKSLKDSLEKLDKVFNAKGVDMTQFPVLQHKEPELVNP 99
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Ensembl Cavefish
Match: ENSAMXT00000012773.2 (uncharacterized LOC103024042 [Source:NCBI gene;Acc:103024042]) HSP 1 Score: 46.595 bits (109), Expect = 2.936e-6 Identity = 27/73 (36.99%), Postives = 42/73 (57.53%), Query Frame = 3 Query: 129 DPIQNLFLKRIREFREKSSKAEFGLVGASAVEIKTFQDSMDRIDAVYHAKGADMESFPQFSFKDENLEDPRPK 347 DPI+ LFL +IRE+ K SKA GLV A + F + + ++ +Y D+ +FP+F F + L++ PK Sbjct: 505 DPIKRLFLDKIREYSTK-SKAHDGLVDAGPEYEREFSEELAKLQRLY--GNGDLTAFPEFEFPEPVLDEFNPK 574
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Ensembl Cavefish
Match: si:ch211-140m22.7 (skin secretory protein xP2-like [Source:NCBI gene;Acc:103025933]) HSP 1 Score: 45.0542 bits (105), Expect = 3.495e-6 Identity = 25/69 (36.23%), Postives = 39/69 (56.52%), Query Frame = 3 Query: 129 DPIQNLFLKRIREFREKSSKAEFGLVGASAVEIKTFQDSMDRIDAVYHAKGADMESFPQFSFKDENLED 335 DPIQ LFL IR++ +S K+ GLV A K + + ++ +Y G D+ SFP+F F + L++ Sbjct: 84 DPIQRLFLDSIRDYSTQS-KSSGGLVDAGPEYQKALAEEIAKLQRLYG--GGDLNSFPEFKFPEPKLDE 149
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Ensembl Cavefish
Match: atp5pf (ATP synthase peripheral stalk subunit F6 [Source:NCBI gene;Acc:103041413]) HSP 1 Score: 43.5134 bits (101), Expect = 5.487e-6 Identity = 23/69 (33.33%), Postives = 38/69 (55.07%), Query Frame = 3 Query: 129 DPIQNLFLKRIREFREKSSKAEFGLVGASAVEIKTFQDSMDRIDAVYHAKGADMESFPQFSFKDENLED 335 DP+Q +F+ +IRE+ K SK+ G+V A K + + ++ +Y G D+ FP F F + LE+ Sbjct: 39 DPVQKMFVDKIREYNSK-SKSAGGVVDAGPSYQKNMTEEITKLQRLYG--GGDLTKFPDFKFPEPKLEE 104
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Ensembl Medaka
Match: si:ch211-140m22.7 (fibrous sheath CABYR-binding protein [Source:NCBI gene;Acc:101171978]) HSP 1 Score: 51.9878 bits (123), Expect = 4.014e-8 Identity = 28/69 (40.58%), Postives = 43/69 (62.32%), Query Frame = 3 Query: 129 DPIQNLFLKRIREFREKSSKAEFGLVGASAVEIKTFQDSMDRIDAVYHAKGADMESFPQFSFKDENLED 335 DPIQ LFL IR++ +S +A GL+ A A KT + + ++ +Y G D+ESFP+FSF + L++ Sbjct: 433 DPIQKLFLDSIRQYSSES-RAAGGLLDAGADYQKTVAEEVAKLQRLYG--GGDLESFPEFSFPEPRLDE 498
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Planmine SMEST
Match: SMESG000038869.1 (SMESG000038869.1) HSP 1 Score: 274.633 bits (701), Expect = 1.767e-96 Identity = 133/133 (100.00%), Postives = 133/133 (100.00%), Query Frame = 3 Query: 45 MILNTVKLSISKLNVIRCFATGSRNPSSDPIQNLFLKRIREFREKSSKAEFGLVGASAVEIKTFQDSMDRIDAVYHAKGADMESFPQFSFKDENLEDPRPKAKLTIPVCYQLEENNKVIDNLLDISNDVDYFH 443 MILNTVKLSISKLNVIRCFATGSRNPSSDPIQNLFLKRIREFREKSSKAEFGLVGASAVEIKTFQDSMDRIDAVYHAKGADMESFPQFSFKDENLEDPRPKAKLTIPVCYQLEENNKVIDNLLDISNDVDYFH Sbjct: 1 MILNTVKLSISKLNVIRCFATGSRNPSSDPIQNLFLKRIREFREKSSKAEFGLVGASAVEIKTFQDSMDRIDAVYHAKGADMESFPQFSFKDENLEDPRPKAKLTIPVCYQLEENNKVIDNLLDISNDVDYFH 133 The following BLAST results are available for this feature:
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 3
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 1
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 3
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 1
BLAST of ATP synthase-coupling factor 6, mitochondrial vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30031354 ID=SMED30031354|Name=ATP synthase-coupling factor 6, mitochondrial|organism=Schmidtea mediterranea sexual|type=transcript|length=469bpback to top protein sequence of SMED30031354-orf-1 >SMED30031354-orf-1 ID=SMED30031354-orf-1|Name=SMED30031354-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=134bp MILNTVKLSISKLNVIRCFATGSRNPSSDPIQNLFLKRIREFREKSSKAEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|