Secreted peptide prohormone 19
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30031317 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 11Note: Hover over icons to view figure legend
Homology
BLAST of Secreted peptide prohormone 19 vs. TrEMBL
Match: E3T7U8 (Secreted peptide prohormone 19 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 143.28 bits (360), Expect = 1.162e-41 Identity = 80/122 (65.57%), Postives = 80/122 (65.57%), Query Frame = 3 Query: 24 MNGTVILCTLIVLLASFPSDGSGRLIIYQYKCFVICNSLAISICL*FTEKRI*LIIYSYFVLIDDLIVKRKHIGHQIFRLKRGYHFFRLRKDEKCLIPGELKSAIKNDLESGSNLCGSDKSF 389 MNGTVILCTLIVLLASFP DGS DDLIVKRKHIGHQIFRLKRGYHFFRLRKDEKCLIPGELKSAIKNDLESGSNLCGSDKSF Sbjct: 1 MNGTVILCTLIVLLASFPLDGS-----------------------------------------DDLIVKRKHIGHQIFRLKRGYHFFRLRKDEKCLIPGELKSAIKNDLESGSNLCGSDKSF 81
BLAST of Secreted peptide prohormone 19 vs. TrEMBL
Match: A0A1I8GSG1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 60.077 bits (144), Expect = 1.884e-7 Identity = 30/58 (51.72%), Postives = 41/58 (70.69%), Query Frame = 3 Query: 222 IVKRKHIGHQIFRLKRGYHFFRLRKDEKCLIPGELKSAIKNDL----ESGSNLCGSDK 383 + + H GHQIFRLK+GYHFFRL+KD++C I GE A+ D+ +SGS LC +D+ Sbjct: 3286 LAEEGHAGHQIFRLKKGYHFFRLKKDQECAI-GEEARALLADIVKSTQSGS-LCPADR 3341
BLAST of Secreted peptide prohormone 19 vs. TrEMBL
Match: A0A267HBD7 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008027g1 PE=4 SV=1) HSP 1 Score: 57.3806 bits (137), Expect = 2.015e-7 Identity = 25/56 (44.64%), Postives = 40/56 (71.43%), Query Frame = 3 Query: 222 IVKRKHIGHQIFRLKRGYHFFRLRKDEKCLIPGELKSAIKNDLESGS--NLCGSDK 383 + + H GHQIFRLK+GYHFFRL+KD++C I E ++ + + ++S +LC +D+ Sbjct: 76 LAEEGHAGHQIFRLKKGYHFFRLKKDQECAIGEEARALLADIVKSAQSGSLCPADR 131
BLAST of Secreted peptide prohormone 19 vs. TrEMBL
Match: E3T7U7 (Gonadotropin-releasing hormone like-1 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 55.8398 bits (133), Expect = 2.567e-7 Identity = 30/65 (46.15%), Postives = 36/65 (55.38%), Query Frame = 3 Query: 195 SYFVLIDDLIVKRKHIGHQIFRLKRGYHFFRLRKDEKCLIPGELKSAIKNDLESGSNLCGSDKSF 389 SY D L KR H GHQ+FRLK+G+H+FRL+K C+ K I S LC SD SF Sbjct: 25 SYHFSNDWLPGKRYHTGHQMFRLKKGFHYFRLKKPSNCIFDNYFKQDILKLARKHSGLCPSDLSF 89
BLAST of Secreted peptide prohormone 19 vs. TrEMBL
Match: A0A267F661 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008027g2 PE=4 SV=1) HSP 1 Score: 56.6102 bits (135), Expect = 4.136e-7 Identity = 30/58 (51.72%), Postives = 41/58 (70.69%), Query Frame = 3 Query: 222 IVKRKHIGHQIFRLKRGYHFFRLRKDEKCLIPGELKSAIKNDL----ESGSNLCGSDK 383 + + H GHQIFRLK+GYHFFRL+KD++C I GE A+ D+ +SGS LC +D+ Sbjct: 86 LAEEGHAGHQIFRLKKGYHFFRLKKDQECAI-GEEARALLADIVKSTQSGS-LCPADR 141
BLAST of Secreted peptide prohormone 19 vs. Planmine SMEST
Match: SMESG000053016.1 (SMESG000053016.1) HSP 1 Score: 142.895 bits (359), Expect = 5.006e-45 Identity = 80/122 (65.57%), Postives = 80/122 (65.57%), Query Frame = 3 Query: 24 MNGTVILCTLIVLLASFPSDGSGRLIIYQYKCFVICNSLAISICL*FTEKRI*LIIYSYFVLIDDLIVKRKHIGHQIFRLKRGYHFFRLRKDEKCLIPGELKSAIKNDLESGSNLCGSDKSF 389 MNGTVILCTLIVLLASFPSDGS DDLIVKRKHIGHQIFRLKRGYHFFRLRKDEKCLIP ELKSAIKNDLESGSNLCGSDKSF Sbjct: 1 MNGTVILCTLIVLLASFPSDGS-----------------------------------------DDLIVKRKHIGHQIFRLKRGYHFFRLRKDEKCLIPVELKSAIKNDLESGSNLCGSDKSF 81
BLAST of Secreted peptide prohormone 19 vs. Planmine SMEST
Match: SMESG000053015.1 (SMESG000053015.1) HSP 1 Score: 89.3521 bits (220), Expect = 3.322e-24 Identity = 55/111 (49.55%), Postives = 57/111 (51.35%), Query Frame = 3 Query: 24 MNGTVILCTLIVLLASFPSDGSGRLIIYQYKCFVICNSLAISICL*FTEKRI*LIIYSYFVLIDDLIVKRKHIGHQIFRLKRGYHFFRLRKDEKCLIPGELKSAIKNDLES 356 MNG VILC LIVLLASFPSDG+ KRK GHQ FR+KRGY FRL KDEKCLIPGELKSAIKNDLES Sbjct: 1 MNGPVILCFLIVLLASFPSDGTD---------------------------------------------KRKKTGHQPFRVKRGYQCFRLGKDEKCLIPGELKSAIKNDLES 66
BLAST of Secreted peptide prohormone 19 vs. Planmine SMEST
Match: SMESG000061019.1 (SMESG000061019.1) HSP 1 Score: 55.8398 bits (133), Expect = 7.063e-11 Identity = 30/65 (46.15%), Postives = 36/65 (55.38%), Query Frame = 3 Query: 195 SYFVLIDDLIVKRKHIGHQIFRLKRGYHFFRLRKDEKCLIPGELKSAIKNDLESGSNLCGSDKSF 389 SY D L KR H GHQ+FRLK+G+H+FRL+K C+ K I S LC SD SF Sbjct: 25 SYHFSNDWLPGKRYHTGHQMFRLKKGFHYFRLKKPSNCIFDNYFKQDILKLARKHSGLCPSDLSF 89
BLAST of Secreted peptide prohormone 19 vs. Planmine SMEST
Match: SMESG000030647.1 (SMESG000030647.1) HSP 1 Score: 50.447 bits (119), Expect = 5.188e-9 Identity = 20/42 (47.62%), Postives = 29/42 (69.05%), Query Frame = 3 Query: 264 KRGYHFFRLRKDEKCLIPGELKSAIKNDLESGSNLCGSDKSF 389 KRGYHFFRL+K C+IP +K+ I+ +++ LC +DK F Sbjct: 27 KRGYHFFRLKKSGDCVIPDVMKAMIETKIQNHEQLCAADKKF 68
BLAST of Secreted peptide prohormone 19 vs. Planmine SMEST
Match: SMESG000073636.1 (SMESG000073636.1) HSP 1 Score: 49.2914 bits (116), Expect = 1.625e-8 Identity = 22/54 (40.74%), Postives = 35/54 (64.81%), Query Frame = 3 Query: 228 KRKHIGHQIFRLKRGYHFFRLRKDEKCLIPGELKSAIKNDLESGSNLCGSDKSF 389 KR GH++FRLK+G+HF +L+++ +C+IP +K I N N+C D+ F Sbjct: 28 KRALTGHRMFRLKKGFHFIKLKRNFECVIPDSVKEEIMN-----GNICLEDQEF 76 The following BLAST results are available for this feature:
BLAST of Secreted peptide prohormone 19 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Secreted peptide prohormone 19 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Secreted peptide prohormone 19 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Secreted peptide prohormone 19 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Secreted peptide prohormone 19 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Secreted peptide prohormone 19 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Secreted peptide prohormone 19 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of Secreted peptide prohormone 19 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Secreted peptide prohormone 19 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Secreted peptide prohormone 19 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Secreted peptide prohormone 19 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Secreted peptide prohormone 19 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Secreted peptide prohormone 19 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Secreted peptide prohormone 19 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30031317 ID=SMED30031317|Name=Secreted peptide prohormone 19|organism=Schmidtea mediterranea sexual|type=transcript|length=466bpback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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