SMED30031277

Overview


Name	SMED30031277
Smed ID	SMED30031277
Length (bp)	442

Neoblast Clusters

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30031277

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
parapharyngeal region	SMED30031277	h1SMcG0011413	SmedASXL_010457	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
pharynx	SMED30031277	h1SMcG0011413	dd_Smed_v4_22156_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
head region	SMED30031277	h1SMcG0011413	dd_Smed_v6_22156_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017

Note: Hover over icons to view figure legend

Alignments

SMED30031277 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.001058:94870..95341 +	2074

Homology

[Close]

BLAST of SMED30031277 vs. Ensembl Human
Match: DCLK1 (doublecortin like kinase 1 [Source:HGNC Symbol;Acc:HGNC:2700])

HSP 1 Score: 95.1301 bits (235), Expect = 6.408e-23
Identity = 51/134 (38.06%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            RY++   IG G+F  V   K   E     E ALKI+ K+   G+E++I NE+ +L R +H  +  L+ + + P E+ LVMEL  GGDL   + S    +E   S ++  L++A+ +L S NI+HR+I  EN+LV
Sbjct:  389 RYKVGRTIGDGNFAVV---KECVERSTAREYALKIIKKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTNKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLV 519

[Close]

BLAST of SMED30031277 vs. Ensembl Human
Match: DCLK1 (doublecortin like kinase 1 [Source:HGNC Symbol;Acc:HGNC:2700])

HSP 1 Score: 95.1301 bits (235), Expect = 6.421e-23
Identity = 51/134 (38.06%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            RY++   IG G+F  V   K   E     E ALKI+ K+   G+E++I NE+ +L R +H  +  L+ + + P E+ LVMEL  GGDL   + S    +E   S ++  L++A+ +L S NI+HR+I  EN+LV
Sbjct:  389 RYKVGRTIGDGNFAVV---KECVERSTAREYALKIIKKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTNKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLV 519

[Close]

BLAST of SMED30031277 vs. Ensembl Human
Match: DCLK1 (doublecortin like kinase 1 [Source:HGNC Symbol;Acc:HGNC:2700])

HSP 1 Score: 92.8189 bits (229), Expect = 2.562e-22
Identity = 51/134 (38.06%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            RY++   IG G+F  V   K   E     E ALKI+ K+   G+E++I NE+ +L R +H  +  L+ + + P E+ LVMEL  GGDL   + S    +E   S ++  L++A+ +L S NI+HR+I  EN+LV
Sbjct:   82 RYKVGRTIGDGNFAVV---KECVERSTAREYALKIIKKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTNKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLV 212

[Close]

BLAST of SMED30031277 vs. Ensembl Human
Match: DCLK1 (doublecortin like kinase 1 [Source:HGNC Symbol;Acc:HGNC:2700])

HSP 1 Score: 92.4337 bits (228), Expect = 3.204e-22
Identity = 51/134 (38.06%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            RY++   IG G+F  V   K   E     E ALKI+ K+   G+E++I NE+ +L R +H  +  L+ + + P E+ LVMEL  GGDL   + S    +E   S ++  L++A+ +L S NI+HR+I  EN+LV
Sbjct:   82 RYKVGRTIGDGNFAVV---KECVERSTAREYALKIIKKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTNKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLV 212

[Close]

BLAST of SMED30031277 vs. Ensembl Human
Match: DCLK2 (doublecortin like kinase 2 [Source:HGNC Symbol;Acc:HGNC:19002])

HSP 1 Score: 92.4337 bits (228), Expect = 4.645e-22
Identity = 51/133 (38.35%), Postives = 78/133 (58.65%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            +Y+I   IG G+F  V   +       +E ALKI+ KA   G+E+LI+NE+ +L R +H  +  L+ + ET  E+ LVMEL  GGDL   + S+   +E   S ++  L+ AL +L   +I+HR+I  EN+LV
Sbjct:  392 KYKIGKVIGDGNFAVV--KECIDRSTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATELFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHGLSIVHRDIKPENLLV 522

[Close]

BLAST of SMED30031277 vs. Ensembl Celegans
Match: F32D8.1 (pep chromosome:WBcel235:V:10880837:10883399:1 gene:WBGene00009326.1 transcript:F32D8.1.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F32D8.1)

HSP 1 Score: 79.7221 bits (195), Expect = 2.413e-18
Identity = 41/133 (30.83%), Postives = 77/133 (57.89%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            +Y+I   IGKG+F  V   +   +E+  + ALK V K  + G+ + +DNE++ML   +H+ +  ++  F T  +  +V E A  GDL   +  NG + E   + +  ++++AL +L  +N++HR++  EN+L+
Sbjct:   70 QYEIGAIIGKGNFSSVHLTRR--KEDGTKCALKQVEKRAMRGKCFFVDNEVEMLSLIQHDHIISIIDAFSTENQYFIVFEHAQYGDLYETIRKNGRIEEPDAAIITLQVASALNYLHERNVVHRDVKPENLLL 200

[Close]

BLAST of SMED30031277 vs. Ensembl Celegans
Match: cmk-1 (Calcium/calmodulin-dependent protein kinase type 1 [Source:UniProtKB/Swiss-Prot;Acc:Q9TXJ0])

HSP 1 Score: 73.9442 bits (180), Expect = 3.258e-16
Identity = 40/132 (30.30%), Postives = 72/132 (54.55%), Query Frame = -1

Query:   23 RYQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENIL 418
            +Y     +G G F +VF  +S ++   +  A+K + K  L G+E  ++NEI +L + RH  +  L   ++  + V LVMEL  GG+L   + + G  +E   S +I ++  A+ F+    ++HR++  EN+L
Sbjct:   21 KYDFRDVLGTGAFSKVFLAESKSDA-GQMYAVKCIDKKALKGKEESLENEIKVLRKLRHNNIVQLFDTYDEKQFVYLVMELVTGGELFDRIVAKGSYTEQDASNLIRQVLEAVGFMHDNGVVHRDLKPENLL 151

[Close]

BLAST of SMED30031277 vs. Ensembl Celegans
Match: par-1 (Serine/threonine-protein kinase par-1 [Source:UniProtKB/Swiss-Prot;Acc:Q9TW45])

HSP 1 Score: 73.9442 bits (180), Expect = 4.800e-16
Identity = 41/137 (29.93%), Postives = 76/137 (55.47%), Query Frame = -1

Query:   20 HIFRYQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYL-IDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 427
            H+ +Y+++  IGKG+F +V   K        EVA+KI+ K  L+      +  E+ ++ +  H  +  L    ET + + LV+E A GG++   L ++G + E +      ++ +A+ +L S+NIIHR++  EN+L+
Sbjct:  102 HVGKYKLLKTIGKGNFAKVKLAKHVIT--GHEVAIKIIDKTALNPSSLQKLFREVKIMKQLDHPNIVKLYQVMETEQTLYLVLEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYLHSKNIIHRDLKAENLLL 236

[Close]

Query:   20 HIFRYQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYL-IDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 427
            H+ +Y+++  IGKG+F +V   K        EVA+KI+ K  L+      +  E+ ++ +  H  +  L    ET + + LV+E A GG++   L ++G + E +      ++ +A+ +L S+NIIHR++  EN+L+
Sbjct:  102 HVGKYKLLKTIGKGNFAKVKLAKHVIT--GHEVAIKIIDKTALNPSSLQKLFREVKIMKQLDHPNIVKLYQVMETEQTLYLVLEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYLHSKNIIHRDLKAENLLL 236

[Close]

Query:   20 HIFRYQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYL-IDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 427
            H+ +Y+++  IGKG+F +V   K        EVA+KI+ K  L+      +  E+ ++ +  H  +  L    ET + + LV+E A GG++   L ++G + E +      ++ +A+ +L S+NIIHR++  EN+L+
Sbjct:  102 HVGKYKLLKTIGKGNFAKVKLAKHVIT--GHEVAIKIIDKTALNPSSLQKLFREVKIMKQLDHPNIVKLYQVMETEQTLYLVLEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYLHSKNIIHRDLKAENLLL 236

[Close]

BLAST of SMED30031277 vs. Ensembl Fly
Match: Nuak1 (gene:FBgn0262617 transcript:FBtr0305351)

HSP 1 Score: 77.7962 bits (190), Expect = 2.982e-17
Identity = 42/135 (31.11%), Postives = 75/135 (55.56%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYL--IDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            R+ II ++G+G + +V  G    +E  +EVA+K + K  ++    L  I  E+ ++    H  + H+   FE  ++++LVME A GG+L   L    VL+E +   +  +++TA+ +     I HR++ LENIL+
Sbjct:   69 RFDIIKKLGQGTYGKVQLG--INKETGQEVAIKTIKKCKIEAEADLVRIRREVQIMSSVHHPNIIHIYEVFENREKMVLVMEFAAGGELYDYLSERKVLTEEEARRIFRQVATAVYYCHKHKICHRDLKLENILL 201

[Close]

BLAST of SMED30031277 vs. Ensembl Fly
Match: Nuak1 (gene:FBgn0262617 transcript:FBtr0305357)

HSP 1 Score: 77.7962 bits (190), Expect = 2.986e-17
Identity = 42/135 (31.11%), Postives = 75/135 (55.56%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYL--IDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            R+ II ++G+G + +V  G    +E  +EVA+K + K  ++    L  I  E+ ++    H  + H+   FE  ++++LVME A GG+L   L    VL+E +   +  +++TA+ +     I HR++ LENIL+
Sbjct:   69 RFDIIKKLGQGTYGKVQLG--INKETGQEVAIKTIKKCKIEAEADLVRIRREVQIMSSVHHPNIIHIYEVFENREKMVLVMEFAAGGELYDYLSERKVLTEEEARRIFRQVATAVYYCHKHKICHRDLKLENILL 201

[Close]

BLAST of SMED30031277 vs. Ensembl Fly
Match: Nuak1 (gene:FBgn0262617 transcript:FBtr0305354)

HSP 1 Score: 77.7962 bits (190), Expect = 3.023e-17
Identity = 42/135 (31.11%), Postives = 75/135 (55.56%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYL--IDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            R+ II ++G+G + +V  G    +E  +EVA+K + K  ++    L  I  E+ ++    H  + H+   FE  ++++LVME A GG+L   L    VL+E +   +  +++TA+ +     I HR++ LENIL+
Sbjct:   69 RFDIIKKLGQGTYGKVQLG--INKETGQEVAIKTIKKCKIEAEADLVRIRREVQIMSSVHHPNIIHIYEVFENREKMVLVMEFAAGGELYDYLSERKVLTEEEARRIFRQVATAVYYCHKHKICHRDLKLENILL 201

[Close]

BLAST of SMED30031277 vs. Ensembl Fly
Match: Nuak1 (gene:FBgn0262617 transcript:FBtr0305353)

HSP 1 Score: 77.7962 bits (190), Expect = 3.023e-17
Identity = 42/135 (31.11%), Postives = 75/135 (55.56%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYL--IDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            R+ II ++G+G + +V  G    +E  +EVA+K + K  ++    L  I  E+ ++    H  + H+   FE  ++++LVME A GG+L   L    VL+E +   +  +++TA+ +     I HR++ LENIL+
Sbjct:   69 RFDIIKKLGQGTYGKVQLG--INKETGQEVAIKTIKKCKIEAEADLVRIRREVQIMSSVHHPNIIHIYEVFENREKMVLVMEFAAGGELYDYLSERKVLTEEEARRIFRQVATAVYYCHKHKICHRDLKLENILL 201

[Close]

BLAST of SMED30031277 vs. Ensembl Fly
Match: Nuak1 (gene:FBgn0262617 transcript:FBtr0305352)

HSP 1 Score: 77.7962 bits (190), Expect = 3.023e-17
Identity = 42/135 (31.11%), Postives = 75/135 (55.56%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYL--IDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            R+ II ++G+G + +V  G    +E  +EVA+K + K  ++    L  I  E+ ++    H  + H+   FE  ++++LVME A GG+L   L    VL+E +   +  +++TA+ +     I HR++ LENIL+
Sbjct:   69 RFDIIKKLGQGTYGKVQLG--INKETGQEVAIKTIKKCKIEAEADLVRIRREVQIMSSVHHPNIIHIYEVFENREKMVLVMEFAAGGELYDYLSERKVLTEEEARRIFRQVATAVYYCHKHKICHRDLKLENILL 201

[Close]

BLAST of SMED30031277 vs. Ensembl Zebrafish
Match: dclk2a (doublecortin-like kinase 2a [Source:ZFIN;Acc:ZDB-GENE-050420-170])

HSP 1 Score: 91.2781 bits (225), Expect = 7.273e-22
Identity = 52/134 (38.81%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            +Y+I   IG G+F  V   K   E    +E ALKI+ K    G+E+LI+NE+ +L R +H  +  L+ + +TP E+ LVMEL  GGDL   + S+   +E   S ++  L+ AL +L    I+HR+I  EN+LV
Sbjct:  419 KYKIGKVIGDGNFAVV---KECVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLIEEVDTPAELYLVMELVKGGDLFDAITSSTKYTEKDASVMVFDLAAALKYLHRMCIVHRDIKPENLLV 549

[Close]

BLAST of SMED30031277 vs. Ensembl Zebrafish
Match: dclk2a (doublecortin-like kinase 2a [Source:ZFIN;Acc:ZDB-GENE-050420-170])

HSP 1 Score: 91.2781 bits (225), Expect = 7.359e-22
Identity = 52/134 (38.81%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            +Y+I   IG G+F  V   K   E    +E ALKI+ K    G+E+LI+NE+ +L R +H  +  L+ + +TP E+ LVMEL  GGDL   + S+   +E   S ++  L+ AL +L    I+HR+I  EN+LV
Sbjct:  391 KYKIGKVIGDGNFAVV---KECVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLIEEVDTPAELYLVMELVKGGDLFDAITSSTKYTEKDASVMVFDLAAALKYLHRMCIVHRDIKPENLLV 521

[Close]

BLAST of SMED30031277 vs. Ensembl Zebrafish
Match: dclk2a (doublecortin-like kinase 2a [Source:ZFIN;Acc:ZDB-GENE-050420-170])

HSP 1 Score: 91.2781 bits (225), Expect = 7.370e-22
Identity = 52/134 (38.81%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            +Y+I   IG G+F  V   K   E    +E ALKI+ K    G+E+LI+NE+ +L R +H  +  L+ + +TP E+ LVMEL  GGDL   + S+   +E   S ++  L+ AL +L    I+HR+I  EN+LV
Sbjct:  400 KYKIGKVIGDGNFAVV---KECVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLIEEVDTPAELYLVMELVKGGDLFDAITSSTKYTEKDASVMVFDLAAALKYLHRMCIVHRDIKPENLLV 530

[Close]

BLAST of SMED30031277 vs. Ensembl Zebrafish
Match: dclk2a (doublecortin-like kinase 2a [Source:ZFIN;Acc:ZDB-GENE-050420-170])

HSP 1 Score: 91.2781 bits (225), Expect = 7.370e-22
Identity = 52/134 (38.81%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            +Y+I   IG G+F  V   K   E    +E ALKI+ K    G+E+LI+NE+ +L R +H  +  L+ + +TP E+ LVMEL  GGDL   + S+   +E   S ++  L+ AL +L    I+HR+I  EN+LV
Sbjct:  400 KYKIGKVIGDGNFAVV---KECVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLIEEVDTPAELYLVMELVKGGDLFDAITSSTKYTEKDASVMVFDLAAALKYLHRMCIVHRDIKPENLLV 530

[Close]

BLAST of SMED30031277 vs. Ensembl Zebrafish
Match: dclk2a (doublecortin-like kinase 2a [Source:ZFIN;Acc:ZDB-GENE-050420-170])

HSP 1 Score: 91.2781 bits (225), Expect = 7.391e-22
Identity = 52/134 (38.81%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            +Y+I   IG G+F  V   K   E    +E ALKI+ K    G+E+LI+NE+ +L R +H  +  L+ + +TP E+ LVMEL  GGDL   + S+   +E   S ++  L+ AL +L    I+HR+I  EN+LV
Sbjct:  419 KYKIGKVIGDGNFAVV---KECVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLIEEVDTPAELYLVMELVKGGDLFDAITSSTKYTEKDASVMVFDLAAALKYLHRMCIVHRDIKPENLLV 549

[Close]

BLAST of SMED30031277 vs. Ensembl Xenopus
Match: arhgap24 (Rho GTPase activating protein 24 [Source:Xenbase;Acc:XB-GENE-996257])

HSP 1 Score: 92.4337 bits (228), Expect = 5.036e-22
Identity = 50/134 (37.31%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            RY++   IG G+F  V   K   E     E ALKI++K+   G+E++I NE+ +L R +H  +  L+ + + P E+ LVMEL  GGDL   + S    +E   + ++  L +A+ +L S NI+HR+I  EN+LV
Sbjct:  386 RYKVGRTIGDGNFAIV---KECIERSTGREYALKIINKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDMPSELYLVMELVKGGDLFDAITSTNKYTERDANGMLYNLMSAIKYLHSLNIVHRDIKPENLLV 516

[Close]

BLAST of SMED30031277 vs. Ensembl Xenopus
Match: arhgap24 (Rho GTPase activating protein 24 [Source:Xenbase;Acc:XB-GENE-996257])

HSP 1 Score: 92.4337 bits (228), Expect = 5.040e-22
Identity = 50/134 (37.31%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            RY++   IG G+F  V   K   E     E ALKI++K+   G+E++I NE+ +L R +H  +  L+ + + P E+ LVMEL  GGDL   + S    +E   + ++  L +A+ +L S NI+HR+I  EN+LV
Sbjct:  402 RYKVGRTIGDGNFAIV---KECIERSTGREYALKIINKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDMPSELYLVMELVKGGDLFDAITSTNKYTERDANGMLYNLMSAIKYLHSLNIVHRDIKPENLLV 532

[Close]

BLAST of SMED30031277 vs. Ensembl Xenopus
Match: arhgap24 (Rho GTPase activating protein 24 [Source:Xenbase;Acc:XB-GENE-996257])

HSP 1 Score: 92.4337 bits (228), Expect = 5.047e-22
Identity = 50/134 (37.31%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            RY++   IG G+F  V   K   E     E ALKI++K+   G+E++I NE+ +L R +H  +  L+ + + P E+ LVMEL  GGDL   + S    +E   + ++  L +A+ +L S NI+HR+I  EN+LV
Sbjct:  402 RYKVGRTIGDGNFAIV---KECIERSTGREYALKIINKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDMPSELYLVMELVKGGDLFDAITSTNKYTERDANGMLYNLMSAIKYLHSLNIVHRDIKPENLLV 532

[Close]

BLAST of SMED30031277 vs. Ensembl Xenopus
Match: arhgap24 (Rho GTPase activating protein 24 [Source:Xenbase;Acc:XB-GENE-996257])

HSP 1 Score: 92.4337 bits (228), Expect = 5.050e-22
Identity = 50/134 (37.31%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            RY++   IG G+F  V   K   E     E ALKI++K+   G+E++I NE+ +L R +H  +  L+ + + P E+ LVMEL  GGDL   + S    +E   + ++  L +A+ +L S NI+HR+I  EN+LV
Sbjct:  402 RYKVGRTIGDGNFAIV---KECIERSTGREYALKIINKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDMPSELYLVMELVKGGDLFDAITSTNKYTERDANGMLYNLMSAIKYLHSLNIVHRDIKPENLLV 532

[Close]

BLAST of SMED30031277 vs. Ensembl Xenopus
Match: arhgap24 (Rho GTPase activating protein 24 [Source:Xenbase;Acc:XB-GENE-996257])

HSP 1 Score: 89.7373 bits (221), Expect = 1.767e-21
Identity = 50/134 (37.31%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEE-NEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            RY++   IG G+F  V   K   E     E ALKI++K+   G+E++I NE+ +L R +H  +  L+ + + P E+ LVMEL  GGDL   + S    +E   + ++  L +A+ +L S NI+HR+I  EN+LV
Sbjct:   49 RYKVGRTIGDGNFAIV---KECIERSTGREYALKIINKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDMPSELYLVMELVKGGDLFDAITSTNKYTERDANGMLYNLMSAIKYLHSLNIVHRDIKPENLLV 179

[Close]

BLAST of SMED30031277 vs. Ensembl Mouse
Match: Dclk1 (doublecortin-like kinase 1 [Source:MGI Symbol;Acc:MGI:1330861])

HSP 1 Score: 94.7449 bits (234), Expect = 5.246e-23
Identity = 51/134 (38.06%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            RY++   IG G+F  V   K   E     E ALKI+ K+   G+E++I NE+ +L R +H  +  L+ + + P E+ LVMEL  GGDL   + S    +E   S ++  L++A+ +L S NI+HR+I  EN+LV
Sbjct:  389 RYKVGRTIGDGNFAVV---KECIERSTAREYALKIIKKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTSKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLV 519

[Close]

BLAST of SMED30031277 vs. Ensembl Mouse
Match: Dclk1 (doublecortin-like kinase 1 [Source:MGI Symbol;Acc:MGI:1330861])

HSP 1 Score: 94.7449 bits (234), Expect = 5.263e-23
Identity = 51/134 (38.06%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            RY++   IG G+F  V   K   E     E ALKI+ K+   G+E++I NE+ +L R +H  +  L+ + + P E+ LVMEL  GGDL   + S    +E   S ++  L++A+ +L S NI+HR+I  EN+LV
Sbjct:  405 RYKVGRTIGDGNFAVV---KECIERSTAREYALKIIKKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTSKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLV 535

[Close]

BLAST of SMED30031277 vs. Ensembl Mouse
Match: Dclk1 (doublecortin-like kinase 1 [Source:MGI Symbol;Acc:MGI:1330861])

HSP 1 Score: 92.0485 bits (227), Expect = 1.991e-22
Identity = 51/134 (38.06%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            RY++   IG G+F  V   K   E     E ALKI+ K+   G+E++I NE+ +L R +H  +  L+ + + P E+ LVMEL  GGDL   + S    +E   S ++  L++A+ +L S NI+HR+I  EN+LV
Sbjct:   82 RYKVGRTIGDGNFAVV---KECIERSTAREYALKIIKKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTSKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLV 212

[Close]

BLAST of SMED30031277 vs. Ensembl Mouse
Match: Dclk1 (doublecortin-like kinase 1 [Source:MGI Symbol;Acc:MGI:1330861])

HSP 1 Score: 92.4337 bits (228), Expect = 2.306e-22
Identity = 51/134 (38.06%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            RY++   IG G+F  V   K   E     E ALKI+ K+   G+E++I NE+ +L R +H  +  L+ + + P E+ LVMEL  GGDL   + S    +E   S ++  L++A+ +L S NI+HR+I  EN+LV
Sbjct:   82 RYKVGRTIGDGNFAVV---KECIERSTAREYALKIIKKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTSKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLV 212

[Close]

BLAST of SMED30031277 vs. Ensembl Mouse
Match: Dclk1 (doublecortin-like kinase 1 [Source:MGI Symbol;Acc:MGI:1330861])

HSP 1 Score: 92.4337 bits (228), Expect = 2.386e-22
Identity = 51/134 (38.06%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            RY++   IG G+F  V   K   E     E ALKI+ K+   G+E++I NE+ +L R +H  +  L+ + + P E+ LVMEL  GGDL   + S    +E   S ++  L++A+ +L S NI+HR+I  EN+LV
Sbjct:   81 RYKVGRTIGDGNFAVV---KECIERSTAREYALKIIKKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTSKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLV 211

[Close]

BLAST of SMED30031277 vs. UniProt/SwissProt
Match: sp|O15075|DCLK1_HUMAN (Serine/threonine-protein kinase DCLK1 OS=Homo sapiens OX=9606 GN=DCLK1 PE=1 SV=2)

HSP 1 Score: 95.1301 bits (235), Expect = 3.084e-22
Identity = 51/134 (38.06%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            RY++   IG G+F  V   K   E     E ALKI+ K+   G+E++I NE+ +L R +H  +  L+ + + P E+ LVMEL  GGDL   + S    +E   S ++  L++A+ +L S NI+HR+I  EN+LV
Sbjct:  389 RYKVGRTIGDGNFAVV---KECVERSTAREYALKIIKKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTNKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLV 519

[Close]

BLAST of SMED30031277 vs. UniProt/SwissProt
Match: sp|Q9JLM8|DCLK1_MOUSE (Serine/threonine-protein kinase DCLK1 OS=Mus musculus OX=10090 GN=Dclk1 PE=1 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 3.681e-22
Identity = 51/134 (38.06%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            RY++   IG G+F  V   K   E     E ALKI+ K+   G+E++I NE+ +L R +H  +  L+ + + P E+ LVMEL  GGDL   + S    +E   S ++  L++A+ +L S NI+HR+I  EN+LV
Sbjct:  405 RYKVGRTIGDGNFAVV---KECIERSTAREYALKIIKKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTSKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLV 535

[Close]

BLAST of SMED30031277 vs. UniProt/SwissProt
Match: sp|Q5MPA9|DCLK2_RAT (Serine/threonine-protein kinase DCLK2 OS=Rattus norvegicus OX=10116 GN=Dclk2 PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 1.472e-21
Identity = 51/133 (38.35%), Postives = 79/133 (59.40%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            +Y+I   IG G+F  V   +       +E ALKI+ KA   G+E+LI+NE+ +L R +H  +  L+ + ET  E+ LVMEL  GGDL   + S+   +E   S ++  L++AL +L   +I+HR+I  EN+LV
Sbjct:  408 KYRIGKVIGDGNFAVV--KECMDRSTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETTTELFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLASALRYLHGLSIVHRDIKPENLLV 538

[Close]

BLAST of SMED30031277 vs. UniProt/SwissProt
Match: sp|O08875|DCLK1_RAT (Serine/threonine-protein kinase DCLK1 OS=Rattus norvegicus OX=10116 GN=Dclk1 PE=1 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 1.898e-21
Identity = 51/134 (38.06%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            RY++   IG G+F  V   K   E     E ALKI+ K+   G+E++I NE+ +L R +H  +  L+ + + P E+ LVMEL  GGDL   + S    +E   S ++  L++A+ +L S NI+HR+I  EN+LV
Sbjct:   82 RYKVGRTIGDGNFAVV---KECIERSTAREYALKIIKKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTSKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLV 212

[Close]

BLAST of SMED30031277 vs. UniProt/SwissProt
Match: sp|D2I3C6|DCLK2_AILME (Serine/threonine-protein kinase DCLK2 OS=Ailuropoda melanoleuca OX=9646 GN=DCLK2 PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 1.973e-21
Identity = 51/133 (38.35%), Postives = 78/133 (58.65%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            +Y+I   IG G+F  V   +       +E ALKI+ KA   G+E+LI+NE+ +L R +H  +  L+ + ET  E+ LVMEL  GGDL   + S+   +E   S ++  L+ AL +L   +I+HR+I  EN+LV
Sbjct:  410 KYKIGKVIGDGNFAVV--KECMDRSTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATELFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHGLSIVHRDIKPENLLV 540

[Close]

BLAST of SMED30031277 vs. TrEMBL
Match: A0A3Q2P4H2 (Doublecortin-like kinase 2a OS=Fundulus heteroclitus OX=8078 PE=4 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 4.327e-25
Identity = 57/140 (40.71%), Postives = 86/140 (61.43%), Query Frame = -1

Query:    2 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILVKSISHH 418
            +Y +   IG G+F  V   K   E    +E ALKI+ KA   G+E+LI+NE+ +L + +H  +  L+ + +TP E+ LVMEL  GGDL   + S+   +E   S ++  L+ ALL+L S NI+HR+I  EN+LV++ SHH
Sbjct:  406 KYSVGKVIGDGNFAVV---KECVERSTGKEFALKIIDKAKCSGKEHLIENEVAVLRKVKHPNIIMLIEELDTPSELCLVMELVKGGDLFDAITSSAKYTEKDASTMVCNLAAALLYLHSMNIVHRDIKPENLLVRTRSHH 542

[Close]

BLAST of SMED30031277 vs. TrEMBL
Match: A0A2B4RRF8 (Serine/threonine-protein kinase DCLK1 OS=Stylophora pistillata OX=50429 GN=Dclk1 PE=4 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 6.804e-25
Identity = 57/136 (41.91%), Postives = 88/136 (64.71%), Query Frame = -1

Query:    8 YQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILVKSIS 415
            Y+I   IG G+F  V   KS   + N+E ALKI+SKA + G+E++++NEI +L R +H+ +  L+ ++ETPKE+ LVMEL  GGDL   +      +E   S ++  L++AL +L S N++HR+I  EN+LV + S
Sbjct:  410 YRIGKVIGDGNFAVVRECKS--RKTNKEYALKIISKAKVKGKEHMVENEISILRRVKHKNIVELIEEYETPKEIFLVMELVKGGDLFEAIVKATKYTEKDASHMVRDLASALHYLHSMNVVHRDIKPENLLVVNYS 543

[Close]

BLAST of SMED30031277 vs. TrEMBL
Match: A0A3M6UBT3 (Uncharacterized protein OS=Pocillopora damicornis OX=46731 GN=pdam_00016118 PE=4 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 1.808e-24
Identity = 56/136 (41.18%), Postives = 87/136 (63.97%), Query Frame = -1

Query:    8 YQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILVKSIS 415
            Y+I   IG G+F  V   K+   + N+E ALKI+SKA + G+E++++NEI +L R +H+ +  L+ ++ETPKE+ LVMEL  GGDL   +      +E   S ++  L+ AL +L S N++HR+I  EN+LV + S
Sbjct:  885 YRIGKVIGDGNFAVVRECKN--RKTNKEYALKIISKAKVKGKEHMVENEISILRRVKHKNIVELIEEYETPKEIFLVMELVKGGDLFEAIVKATKYTEKDASHMVRDLAAALHYLHSMNVVHRDIKPENLLVVNYS 1018

[Close]

BLAST of SMED30031277 vs. TrEMBL
Match: A7RVP7 (Predicted protein (Fragment) OS=Nematostella vectensis OX=45351 GN=v1g95129 PE=3 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 4.383e-23
Identity = 53/132 (40.15%), Postives = 84/132 (63.64%), Query Frame = -1

Query:   20 YQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 415
            Y+I   IG G+F  V   K    + N+E ALKI++KA + G+E++I+NEI +L R +H  +  L+ ++ETP+E+ LVMEL  GGDL   +      +E+  S ++  L++AL +L   +I+HR+I  EN+LV
Sbjct:    1 YRIGKVIGDGNFAVVRECKH--RKTNKEYALKIINKAKVKGKEHMIENEISILRRVKHNHIVELIEEYETPREIFLVMELVKGGDLFEAIVEATKYTEVHASHMVRDLASALDYLHCNSIVHRDIKPENLLV 130

[Close]

BLAST of SMED30031277 vs. TrEMBL
Match: A0A0P0CGZ3 (Doublecortin-like kinase OS=Holothuria glaberrima OX=31192 GN=DCLK PE=2 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 8.118e-23
Identity = 55/133 (41.35%), Postives = 79/133 (59.40%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            +Y I   IG G+F  V   K    +  EE ALKI++KA   G+E +I+NE+ +L R  H  +  L+ +F+ P E+ LVMEL  GGDL   + +    +E   SC+I  L +AL +L S NI+HR+I  EN+LV
Sbjct:  387 KYDIKELIGNGNFAVV--RKCTDRDTGEEFALKIINKAKCKGKEQMIENEVSILRRVNHVNIILLIEEFDGPTELYLVMELVQGGDLFDAISTTTKYTERDASCMIHNLFSALQYLHSMNIVHRDIKPENLLV 517

[Close]

BLAST of SMED30031277 vs. Ensembl Cavefish
Match: ENSAMXT00000039497.1 (pep primary_assembly:Astyanax_mexicanus-2.0:15:8818577:8831816:1 gene:ENSAMXG00000040052.1 transcript:ENSAMXT00000039497.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 94.3597 bits (233), Expect = 5.453e-23
Identity = 53/134 (39.55%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            +Y++   IG G+F  V   K   E    +E ALKI+ KA   G+E+LI NE+ +L R RH  +  L+ + +TP E+ LVMEL  GGDL   + S+   +E   S ++  L+ AL +L    I+HR+I  EN+LV
Sbjct:  383 KYKVGKVIGDGNFAVV---KECVERATGQEYALKIIDKAKCSGKEHLIANEVAILRRVRHPSIIMLIEEVDTPTELYLVMELVKGGDLFDAITSSTKYTERDASAMVYNLTGALRYLHRMCIVHRDIKPENLLV 513

[Close]

BLAST of SMED30031277 vs. Ensembl Cavefish
Match: ENSAMXT00000043405.1 (pep primary_assembly:Astyanax_mexicanus-2.0:15:8818577:8831816:1 gene:ENSAMXG00000040052.1 transcript:ENSAMXT00000043405.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 92.8189 bits (229), Expect = 2.008e-22
Identity = 53/134 (39.55%), Postives = 78/134 (58.21%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            +Y++   IG G+F  V   K   E    +E ALKI+ KA   G+E+LI NE+ +L R RH  +  L+ + +TP E+ LVMEL  GGDL   + S+   +E   S ++  L+ AL +L    I+HR+I  EN+LV
Sbjct:  369 KYKVGKVIGDGNFAVV---KECVERATGQEYALKIIDKAKCSGKEHLIANEVAILRRVRHPSIIMLIEEVDTPTELYLVMELVKGGDLFDAITSSTKYTERDASAMVYNLTGALRYLHRMCIVHRDIKPENLLV 499

[Close]

BLAST of SMED30031277 vs. Ensembl Cavefish
Match: dclk2a (doublecortin like kinase 2 [Source:NCBI gene;Acc:103023338])

HSP 1 Score: 92.0485 bits (227), Expect = 4.234e-22
Identity = 51/134 (38.06%), Postives = 79/134 (58.96%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            +Y++   IG G+F  V   K   E    +E ALKI+ K    G+E+LI+NE+ +L R +H  +  L+ + +TP E+ LVMEL  GGDL   + S+   +E   S ++  L+ AL +L   +I+HR+I  EN+LV
Sbjct:  425 KYKVGKIIGDGNFAVV---KDCVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLMEEVDTPSELYLVMELVKGGDLFDSITSSTKYTERDASAMVYNLAGALKYLHRMSIVHRDIKPENLLV 555

[Close]

BLAST of SMED30031277 vs. Ensembl Cavefish
Match: dclk2a (doublecortin like kinase 2 [Source:NCBI gene;Acc:103023338])

HSP 1 Score: 92.0485 bits (227), Expect = 4.251e-22
Identity = 51/134 (38.06%), Postives = 79/134 (58.96%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            +Y++   IG G+F  V   K   E    +E ALKI+ K    G+E+LI+NE+ +L R +H  +  L+ + +TP E+ LVMEL  GGDL   + S+   +E   S ++  L+ AL +L   +I+HR+I  EN+LV
Sbjct:  441 KYKVGKIIGDGNFAVV---KDCVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLMEEVDTPSELYLVMELVKGGDLFDSITSSTKYTERDASAMVYNLAGALKYLHRMSIVHRDIKPENLLV 571

[Close]

BLAST of SMED30031277 vs. Ensembl Cavefish
Match: dclk2a (doublecortin like kinase 2 [Source:NCBI gene;Acc:103023338])

HSP 1 Score: 92.0485 bits (227), Expect = 4.315e-22
Identity = 51/134 (38.06%), Postives = 79/134 (58.96%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            +Y++   IG G+F  V   K   E    +E ALKI+ K    G+E+LI+NE+ +L R +H  +  L+ + +TP E+ LVMEL  GGDL   + S+   +E   S ++  L+ AL +L   +I+HR+I  EN+LV
Sbjct:  441 KYKVGKIIGDGNFAVV---KDCVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLMEEVDTPSELYLVMELVKGGDLFDSITSSTKYTERDASAMVYNLAGALKYLHRMSIVHRDIKPENLLV 571

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BLAST of SMED30031277 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010548.1 (pep scaffold:Pmarinus_7.0:GL477749:266235:284843:-1 gene:ENSPMAG00000009555.1 transcript:ENSPMAT00000010548.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 83.9593 bits (206), Expect = 5.422e-20
Identity = 47/141 (33.33%), Postives = 78/141 (55.32%), Query Frame = -1

Query:   17 RYQIITQIGKGDFC------RVFYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRC-RHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILVK 418
            RY+I   IG G+F       RV  G  +  E   ++A+KI+ KA L GRE ++ +E+ +  R  +H  V  L+   E+P +  LV+E   GGDL   +  +   +E   + +++ L  AL +L + NI+HR++  EN+LV+
Sbjct:  362 RYEIGRTIGDGNFATVKECRRVSSGDGSAGEAEWDLAMKIIDKAKLVGREEMLRSEVALTTRLGQHPNVVRLVEAMESPSDAYLVLERVRGGDLFDAITESVRFTERHAAAMVADLCRALQYLHANNIVHRDVKPENLLVQ 502

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BLAST of SMED30031277 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008023.1 (pep scaffold:Pmarinus_7.0:GL476334:542398:543161:1 gene:ENSPMAG00000007258.1 transcript:ENSPMAT00000008023.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 75.8702 bits (185), Expect = 6.969e-18
Identity = 46/146 (31.51%), Postives = 74/146 (50.68%), Query Frame = -1

Query:   17 RYQIITQIGKGDFCRV-----------FYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRC-RHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILVK 418
            RY+I   IG G+F  V                +T     ++A+KIV K+ L GRE +++NE+ +  R   H  V  LL   ETP    LV+E   GGDL   +  +   +E   + +++ L  AL +L +  ++HR++  EN+LV+
Sbjct:   98 RYEIGRTIGDGNFAVVKECRRRVGVGVGVEGRSTPPGEWDLAMKIVDKSKLAGREEMLENEVTLTARLGHHPNVVRLLESLETPSHAYLVLERVSGGDLFDAITESVRFTERHAAAMVADLCRALQYLHANLVVHRDVKPENLLVR 243

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BLAST of SMED30031277 vs. Ensembl Sea Lamprey
Match: stk33 (serine/threonine kinase 33 [Source:ZFIN;Acc:ZDB-GENE-040724-242])

HSP 1 Score: 74.7146 bits (182), Expect = 3.345e-17
Identity = 50/139 (35.97%), Postives = 75/139 (53.96%), Query Frame = -1

Query:    2 YQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGRE-YLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILVKSISHH 415
            Y++  Q+G G F  V+  ++   E   + A+K V+K         L++ E+ +L R +H+ + HL    ET K V LVMEL   G+L  VLE    LSE +   V   L+ A+ +L   +I+HR++ LENIL KS   H
Sbjct:    5 YRLGRQLGSGSFGTVY--EATHVETGVKWAIKTVNKERAGSSGVRLLEREVVILKRVQHQNIIHLEEVLETHKRVYLVMELCELGELRAVLEQRKRLSEAEARHVAHSLAGAIAYLHKHDIVHRDLKLENILAKSSEPH 141

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BLAST of SMED30031277 vs. Ensembl Sea Lamprey
Match: STK36 (serine/threonine kinase 36 [Source:HGNC Symbol;Acc:HGNC:17209])

HSP 1 Score: 73.9442 bits (180), Expect = 1.512e-16
Identity = 45/134 (33.58%), Postives = 76/134 (56.72%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYL-IDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            +Y ++  IG+G F +V+ G+       + VALK + K     +E   +  EID++   +H  +  +L  FET KEV++V + A  G+L  +LE +G L E Q   +  +L +AL +L S  I+HR++  +NIL+
Sbjct:    3 KYHVLEMIGEGSFGKVYKGRK--RYTGQVVALKFIPKVGRSDKELRNLRREIDIMRGLQHPNIVQMLDSFETDKEVVVVTDYA-EGELFQILEDDGNLPEEQVREIACQLVSALYYLHSHRILHRDMKPQNILL 133

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BLAST of SMED30031277 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007114.1 (pep scaffold:Pmarinus_7.0:GL477366:299514:356138:-1 gene:ENSPMAG00000006368.1 transcript:ENSPMAT00000007114.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 69.707 bits (169), Expect = 4.176e-15
Identity = 43/140 (30.71%), Postives = 79/140 (56.43%), Query Frame = -1

Query:    2 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILVKSISHH 418
            +Y+I   IG G+F  V   +   E        L I+ +     +E++I++E+ +L R +H  +  L+ + +TP E+ +VMEL  GGDL   + S+   +E   S ++  L++A+ +L + N I+R+I  EN+LV++  H+
Sbjct:  413 KYKIGKVIGDGNFAVV---RECIERMMIASYMLNIIQRHPPRPQEHMIESEVSILRRVKHPNIILLVEEVDTPSELYMVMELVKGGDLFDAITSSTKYTERDASGMLYNLTSAVAYLHALN-INRDIKPENLLVRAGMHY 548

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BLAST of SMED30031277 vs. Ensembl Yeast
Match: DBF20 (Ser/Thr kinase involved in late nuclear division; one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; DBF20 has a paralog, DBF2, that arose from the whole genome duplication [Source:SGD;Acc:S000006315])

HSP 1 Score: 73.9442 bits (180), Expect = 1.040e-16
Identity = 43/140 (30.71%), Postives = 77/140 (55.00%), Query Frame = -1

Query:    5 YQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALL---DGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILVKSISH 415
            +QI+TQ+G+G + +V+  K   ++ +E  ALKI++K LL   +   +++  E D+L   R +++  LL+ F+ P+ + L ME  PGGD   +L +  +L        IS++  A+  L      HR++  EN L+ +  H
Sbjct:  169 FQILTQVGQGGYGQVYLAKK--KDSDEICALKILNKKLLFKLNETNHVL-TERDILTTTRSDWLVKLLYAFQDPESLYLAMEFVPGGDFRTLLINTRILKSGHARFYISEMFCAVNALHELGYTHRDLKPENFLIDATGH 305

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BLAST of SMED30031277 vs. Ensembl Yeast
Match: DBF2 (Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis; DBF2 has a paralog, DBF20, that arose from the whole genome duplication [Source:SGD;Acc:S000003324])

HSP 1 Score: 66.2402 bits (160), Expect = 5.037e-14
Identity = 41/140 (29.29%), Postives = 75/140 (53.57%), Query Frame = -1

Query:    5 YQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALL---DGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILVKSISH 415
            +++ITQ+G+G + +V+  +   ++  E  ALKI++K LL   +  ++++  E D+L   R E++  LL+ F+  + + L ME  PGGD   +L +   L        IS++  A+  L      HR++  EN L+ +  H
Sbjct:  177 FEMITQVGQGGYGQVYLARK--KDTKEVCALKILNKKLLFKLNETKHVL-TERDILTTTRSEWLVKLLYAFQDLQSLYLAMEFVPGGDFRTLLINTRCLKSGHARFYISEMFCAVNALHDLGYTHRDLKPENFLIDAKGH 313

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BLAST of SMED30031277 vs. Ensembl Yeast
Match: CMK1 (Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication [Source:SGD;Acc:S000001910])

HSP 1 Score: 62.7734 bits (151), Expect = 6.455e-13
Identity = 39/135 (28.89%), Postives = 72/135 (53.33%), Query Frame = -1

Query:   23 RYQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYLID---NEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENIL 418
            +Y     +G G F  V   K+   E  E+VA+KI+ K  L G +  ++   +E+D+L R  H  +      FE+  +  ++ +LA GG+L   +   G  +E     ++ ++ +A+ ++ SQNI+HR++  EN+L
Sbjct:   36 KYVFGKTLGAGTFGVVRQAKNT--ETGEDVAVKILIKKALKGNKVQLEALYDELDILQRLHHPNIVAFKDWFESKDKFYIITQLAKGGELFDRILKKGKFTEEDAVRILVEILSAVKYMHSQNIVHRDLKPENLL 168

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BLAST of SMED30031277 vs. Ensembl Yeast
Match: SNF1 (AMP-activated S/T protein kinase; forms a complex with Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis; regulates nucleocytoplasmic shuttling of Hxk2p; regulates filamentous growth and acts as a non-canonical GEF, activating Arf3p during invasive growth; SUMOylation by Mms21p inhibits its function and targets Snf1p for destruction via the Slx5-Slx8 Ub ligase [Source:SGD;Acc:S000002885])

HSP 1 Score: 59.6918 bits (143), Expect = 8.646e-12
Identity = 42/141 (29.79%), Postives = 72/141 (51.06%), Query Frame = -1

Query:   20 HIFRYQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALL-----DGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 427
            HI  YQI+  +G+G F +V      T    ++VALKI++K +L      GR   I+ EI  L   RH  +  L    ++  E+I+V+E A G +L   +     +SE +      ++ +A+ +     I+HR++  EN+L+
Sbjct:   51 HIGNYQIVKTLGEGSFGKVKLAYHTTT--GQKVALKIINKKVLAKSDMQGR---IEREISYLRLLRHPHIIKLYDVIKSKDEIIMVIEYA-GNELFDYIVQRDKMSEQEARRFFQQIISAVEYCHRHKIVHRDLKPENLLL 185

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BLAST of SMED30031277 vs. Ensembl Yeast
Match: PKH3 (Protein kinase with similarity to mammalian PDK1 and yeast Pkh1p/Phk2p; yeast Pkh1p and Pkh2p are two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant [Source:SGD;Acc:S000002874])

HSP 1 Score: 59.6918 bits (143), Expect = 9.041e-12
Identity = 37/132 (28.03%), Postives = 69/132 (52.27%), Query Frame = -1

Query:   20 QIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGR---EYLI--DNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 400
            ++G G +  VF      +  N+  A+K+ SK  +      +Y+    N +++L +  H  +  L + F   + +  V++ APGG+LL +L   G  +++ T    ++L  AL F+ S  IIHR++  EN+L+
Sbjct:   16 ELGHGSYSTVFKALD-KKSPNKIYAIKVCSKKHIIKEAKVKYVTIEKNTMNLLAQKHHAGIIKLYYTFHDEENLYFVLDFAPGGELLSLLHKMGTFNDIWTRHFTAQLIDALEFIHSHGIIHRDLKPENVLL 146

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BLAST of SMED30031277 vs. Ensembl Nematostella
Match: EDO44488 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVP7])

HSP 1 Score: 100.523 bits (249), Expect = 6.061e-27
Identity = 53/132 (40.15%), Postives = 84/132 (63.64%), Query Frame = -1

Query:   20 YQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 415
            Y+I   IG G+F  V   K    + N+E ALKI++KA + G+E++I+NEI +L R +H  +  L+ ++ETP+E+ LVMEL  GGDL   +      +E+  S ++  L++AL +L   +I+HR+I  EN+LV
Sbjct:    1 YRIGKVIGDGNFAVVRECKH--RKTNKEYALKIINKAKVKGKEHMIENEISILRRVKHNHIVELIEEYETPREIFLVMELVKGGDLFEAIVEATKYTEVHASHMVRDLASALDYLHCNSIVHRDIKPENLLV 130

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BLAST of SMED30031277 vs. Ensembl Nematostella
Match: EDO42432 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1C2])

HSP 1 Score: 84.3445 bits (207), Expect = 5.686e-20
Identity = 44/132 (33.33%), Postives = 72/132 (54.55%), Query Frame = -1

Query:   20 YQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 415
            Y    ++G G+F  V   K    ++  E A+KI+ K  + G+E +ID+EI ++ RCRH  +  L   +++  E+ LVMEL  GGDL   + S+   +E         +  AL +L  + I+HR++  EN+LV
Sbjct:  246 YNFGQKLGDGNFAIVRQCKDKITQK--EFAIKIIDKRKIRGKEKMIDDEIAIMRRCRHPNIVRLFEDYDSATEIYLVMELIKGGDLFDAISSSVKFTEHVAKSYFRDMCKALAYLHKRKIVHRDLKPENLLV 375

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BLAST of SMED30031277 vs. Ensembl Nematostella
Match: EDO31543 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SXJ7])

HSP 1 Score: 80.1073 bits (196), Expect = 1.127e-19
Identity = 49/133 (36.84%), Postives = 75/133 (56.39%), Query Frame = -1

Query:   20 YQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREY-LIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 415
            Y I+  IG+G F +V+ G+   +     VALK + K     +E   +  EID++   +HE +  LL  FETP+EV +V E A  G+L  +LE +G L E Q   +  +L  AL +L S  I+HR++  +NIL+
Sbjct:    4 YHILELIGEGSFGKVYKGRR--KYTGNFVALKFIPKTGRSEKELKCLQREIDIMRGLQHENIIRLLDSFETPQEVCVVTEYA-AGELFQILEDDGALPEEQVQRIACQLVKALYYLHSHRILHRDMKPQNILI 133

[Close]

BLAST of SMED30031277 vs. Ensembl Nematostella
Match: EDO30768 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZV3])

HSP 1 Score: 70.8626 bits (172), Expect = 3.053e-15
Identity = 42/139 (30.22%), Postives = 75/139 (53.96%), Query Frame = -1

Query:   14 HIFRYQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYL-IDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILVKS 427
            HI RY++I  IGKG+F +V   K       +EVA+KI+ K  L+      +  E+ ++    H  +  L    ET K + LVME A GG++   L ++G + E +      ++ +A+ +   +++IHR++  EN+L+ +
Sbjct:   34 HIGRYRLIKTIGKGNFAKVKLAKHVPT--GKEVAIKIIDKTQLNPSSLQKLFREVRIMKFLDHPNIVKLYEVIETDKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKHVIHRDLKAENLLLDA 170

[Close]

BLAST of SMED30031277 vs. Ensembl Nematostella
Match: EDO47961 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RKT6])

HSP 1 Score: 67.781 bits (164), Expect = 1.380e-14
Identity = 40/136 (29.41%), Postives = 71/136 (52.21%), Query Frame = -1

Query:   14 YQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSK--ALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILVKS 415
            Y ++  +G+G F +V   KS  ++ N  VA+KI+ K  A  D     +  EI ++ +  H  V  L    ET  +V L++ELA GGDLL  +  N +L E +   +  +    + +   + ++HR++  EN+L+ +
Sbjct:    8 YTVMRNLGEGAFAKVKLAKS--KKHNCHVAIKIIDKRKAPKDYIYKFLPREIRVMHKLNHPNVIQLYEAIETETKVYLILELAEGGDLLEYINKNALLPEERARVIFCQFVATMAYCHKERVVHRDLKCENLLLDA 141

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BLAST of SMED30031277 vs. Ensembl Medaka
Match: ENSORLT00000003850.2 (serine/threonine-protein kinase DCLK2 [Source:NCBI gene;Acc:101173659])

HSP 1 Score: 97.8265 bits (242), Expect = 3.265e-24
Identity = 54/134 (40.30%), Postives = 79/134 (58.96%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            +Y++   IG G+F  V   K   E    +E ALKI+ KA   G+E+LI+NE+ +L R RH  +  L+   ETP ++ LVMEL  GGDL   + S+   SE   S ++  L+ A+ +L   NI+HR+I  EN+LV
Sbjct:  427 KYKVGKVIGDGNFAVV---KECVERATGQEFALKIIDKARCSGKEHLIENEVAVLRRVRHPSIIQLIEVDETPSQLFLVMELVKGGDLFDAITSSTKYSERDASAMVYNLAGAVKYLHRMNIVHRDIKPENLLV 557

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BLAST of SMED30031277 vs. Ensembl Medaka
Match: ENSORLT00000031333.1 (serine/threonine-protein kinase DCLK2 [Source:NCBI gene;Acc:101173659])

HSP 1 Score: 97.8265 bits (242), Expect = 3.265e-24
Identity = 54/134 (40.30%), Postives = 79/134 (58.96%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEEN-EEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            +Y++   IG G+F  V   K   E    +E ALKI+ KA   G+E+LI+NE+ +L R RH  +  L+   ETP ++ LVMEL  GGDL   + S+   SE   S ++  L+ A+ +L   NI+HR+I  EN+LV
Sbjct:  427 KYKVGKVIGDGNFAVV---KECVERATGQEFALKIIDKARCSGKEHLIENEVAVLRRVRHPSIIQLIEVDETPSQLFLVMELVKGGDLFDAITSSTKYSERDASAMVYNLAGAVKYLHRMNIVHRDIKPENLLV 557

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BLAST of SMED30031277 vs. Ensembl Medaka
Match: dclk1b (serine/threonine-protein kinase DCLK1 [Source:NCBI gene;Acc:101155370])

HSP 1 Score: 97.0561 bits (240), Expect = 6.365e-24
Identity = 49/133 (36.84%), Postives = 79/133 (59.40%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            RY++   +G G+F  V   +        E ALKI+SK    G+E++I +E+ +L R +H  +  L+ + ++P E+ LVMEL  GGDL   + S+   +E   SC++  L+ A+ +L S NI+HR+I  EN+LV
Sbjct:  385 RYKVGRTLGDGNFAVV--RECVERSTGREYALKIISKEKCKGKEHMIQSEVAILRRVKHPNIVLLVEEIDSPSELYLVMELVKGGDLFYAITSSNKYTERDASCMLFNLANAIKYLHSLNIVHRDIKPENLLV 515

[Close]

BLAST of SMED30031277 vs. Ensembl Medaka
Match: dclk1b (serine/threonine-protein kinase DCLK1 [Source:NCBI gene;Acc:101155370])

HSP 1 Score: 97.0561 bits (240), Expect = 6.387e-24
Identity = 49/133 (36.84%), Postives = 79/133 (59.40%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            RY++   +G G+F  V   +        E ALKI+SK    G+E++I +E+ +L R +H  +  L+ + ++P E+ LVMEL  GGDL   + S+   +E   SC++  L+ A+ +L S NI+HR+I  EN+LV
Sbjct:  385 RYKVGRTLGDGNFAVV--RECVERSTGREYALKIISKEKCKGKEHMIQSEVAILRRVKHPNIVLLVEEIDSPSELYLVMELVKGGDLFYAITSSNKYTERDASCMLFNLANAIKYLHSLNIVHRDIKPENLLV 515

[Close]

BLAST of SMED30031277 vs. Ensembl Medaka
Match: DCLK2 (doublecortin like kinase 2 [Source:NCBI gene;Acc:101175053])

HSP 1 Score: 94.7449 bits (234), Expect = 4.869e-23
Identity = 52/139 (37.41%), Postives = 80/139 (57.55%), Query Frame = -1

Query:    2 RYQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILVKSISHH 418
            +Y++   IG G+F  V   +       +E ALKI+ KA   G+E+LI+NE+ +L + +H  +  L+ +  TP E+ LVMEL   GDL   + S+   +E   S ++  L+ AL +L S NI+HR+I  EN+LV    HH
Sbjct:  338 KYEVGKVIGDGNFAVV--RECVERSTGKEFALKIIDKARCRGKEHLIENEVAVLRKVKHPNIVMLVEEMVTPSELCLVMELVKDGDLFDAITSSAKYTERDASMMVCNLAGALKYLHSMNIVHRDIKPENLLV--YEHH 472

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BLAST of SMED30031277 vs. Planmine SMEST
Match: SMESG000040958.1 (SMESG000040958.1)

HSP 1 Score: 281.182 bits (718), Expect = 2.447e-93
Identity = 137/138 (99.28%), Postives = 138/138 (100.00%), Query Frame = -1

Query:    2 YQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILVKSISHH 415
            YQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKE+ILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILVKSISHH
Sbjct:  222 YQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEIILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILVKSISHH 359

[Close]

BLAST of SMED30031277 vs. Planmine SMEST
Match: SMESG000040958.1 (SMESG000040958.1)

HSP 1 Score: 281.182 bits (718), Expect = 1.656e-90
Identity = 137/138 (99.28%), Postives = 138/138 (100.00%), Query Frame = -1

Query:    2 YQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILVKSISHH 415
            YQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKE+ILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILVKSISHH
Sbjct:  554 YQIITQIGKGDFCRVFYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEIILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILVKSISHH 691

[Close]

BLAST of SMED30031277 vs. Planmine SMEST
Match: SMESG000078466.1 (SMESG000078466.1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.124e-21
Identity = 47/136 (34.56%), Postives = 81/136 (59.56%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRV---FYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            R+++   +G G+F  V    + K+ TE      ALKI+ K+ + G+E +I+NEI ++  C H+ +  L  ++ET +EV LVMEL   GDL   +  N   SE   + +++ ++ AL +L S+ I+HR++  EN++V
Sbjct:  745 RFEVTKMLGDGNFAVVKLALHRKTKTE-----YALKIIDKSRMMGKESMIENEIWIMKLCNHDNIVKLKEEYETREEVFLVMELVKNGDLFDAITQNVKFSEPVAAKMVADMANALFYLHSRRIVHRDLKPENLMV 875

[Close]

BLAST of SMED30031277 vs. Planmine SMEST
Match: SMESG000078466.1 (SMESG000078466.1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.235e-21
Identity = 47/136 (34.56%), Postives = 81/136 (59.56%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRV---FYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            R+++   +G G+F  V    + K+ TE      ALKI+ K+ + G+E +I+NEI ++  C H+ +  L  ++ET +EV LVMEL   GDL   +  N   SE   + +++ ++ AL +L S+ I+HR++  EN++V
Sbjct:  771 RFEVTKMLGDGNFAVVKLALHRKTKTE-----YALKIIDKSRMMGKESMIENEIWIMKLCNHDNIVKLKEEYETREEVFLVMELVKNGDLFDAITQNVKFSEPVAAKMVADMANALFYLHSRRIVHRDLKPENLMV 901

[Close]

BLAST of SMED30031277 vs. Planmine SMEST
Match: SMESG000078466.1 (SMESG000078466.1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.436e-21
Identity = 47/136 (34.56%), Postives = 81/136 (59.56%), Query Frame = -1

Query:   20 RYQIITQIGKGDFCRV---FYGKSATEEENEEVALKIVSKALLDGREYLIDNEIDMLLRCRHEFVAHLLHQFETPKEVILVMELAPGGDLLVVLESNGVLSELQTSCVISKLSTALLFLQSQNIIHRNISLENILV 418
            R+++   +G G+F  V    + K+ TE      ALKI+ K+ + G+E +I+NEI ++  C H+ +  L  ++ET +EV LVMEL   GDL   +  N   SE   + +++ ++ AL +L S+ I+HR++  EN++V
Sbjct:  762 RFEVTKMLGDGNFAVVKLALHRKTKTE-----YALKIIDKSRMMGKESMIENEIWIMKLCNHDNIVKLKEEYETREEVFLVMELVKNGDLFDAITQNVKFSEPVAAKMVADMANALFYLHSRRIVHRDLKPENLMV 892

The following BLAST results are available for this feature:

BLAST of SMED30031277 vs. Ensembl Human
BLAST of SMED30031277 vs. Ensembl Celegans
BLAST of SMED30031277 vs. Ensembl Fly
BLAST of SMED30031277 vs. Ensembl Zebrafish
BLAST of SMED30031277 vs. Ensembl Xenopus
BLAST of SMED30031277 vs. Ensembl Mouse
BLAST of SMED30031277 vs. UniProt/SwissProt
BLAST of SMED30031277 vs. TrEMBL
BLAST of SMED30031277 vs. Ensembl Cavefish
BLAST of SMED30031277 vs. Ensembl Sea Lamprey
BLAST of SMED30031277 vs. Ensembl Yeast
BLAST of SMED30031277 vs. Ensembl Nematostella
BLAST of SMED30031277 vs. Ensembl Medaka
BLAST of SMED30031277 vs. Planmine SMEST

BLAST of SMED30031277 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
DCLK1	6.408e-23	38.06	doublecortin like kinase 1 [Source:HGNC Symbol;Acc...	[more]
DCLK1	6.421e-23	38.06	doublecortin like kinase 1 [Source:HGNC Symbol;Acc...	[more]
DCLK1	2.562e-22	38.06	doublecortin like kinase 1 [Source:HGNC Symbol;Acc...	[more]
DCLK1	3.204e-22	38.06	doublecortin like kinase 1 [Source:HGNC Symbol;Acc...	[more]
DCLK2	4.645e-22	38.35	doublecortin like kinase 2 [Source:HGNC Symbol;Acc...	[more]

BLAST of SMED30031277 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
F32D8.1	2.413e-18	30.83	pep chromosome:WBcel235:V:10880837:10883399:1 gene...	[more]
cmk-1	3.258e-16	30.30	Calcium/calmodulin-dependent protein kinase type 1...	[more]
par-1	4.800e-16	29.93	Serine/threonine-protein kinase par-1 [Source:Uni...	[more]
par-1	4.800e-16	29.93	Serine/threonine-protein kinase par-1 [Source:Uni...	[more]
par-1	4.800e-16	29.93	Serine/threonine-protein kinase par-1 [Source:Uni...	[more]

BLAST of SMED30031277 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Nuak1	2.982e-17	31.11	gene:FBgn0262617 transcript:FBtr0305351	[more]
Nuak1	2.986e-17	31.11	gene:FBgn0262617 transcript:FBtr0305357	[more]
Nuak1	3.023e-17	31.11	gene:FBgn0262617 transcript:FBtr0305354	[more]
Nuak1	3.023e-17	31.11	gene:FBgn0262617 transcript:FBtr0305353	[more]
Nuak1	3.023e-17	31.11	gene:FBgn0262617 transcript:FBtr0305352	[more]

BLAST of SMED30031277 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
dclk2a	7.273e-22	38.81	doublecortin-like kinase 2a [Source:ZFIN;Acc:ZDB-G...	[more]
dclk2a	7.359e-22	38.81	doublecortin-like kinase 2a [Source:ZFIN;Acc:ZDB-G...	[more]
dclk2a	7.370e-22	38.81	doublecortin-like kinase 2a [Source:ZFIN;Acc:ZDB-G...	[more]
dclk2a	7.370e-22	38.81	doublecortin-like kinase 2a [Source:ZFIN;Acc:ZDB-G...	[more]
dclk2a	7.391e-22	38.81	doublecortin-like kinase 2a [Source:ZFIN;Acc:ZDB-G...	[more]

BLAST of SMED30031277 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
arhgap24	5.036e-22	37.31	Rho GTPase activating protein 24 [Source:Xenbase;A...	[more]
arhgap24	5.040e-22	37.31	Rho GTPase activating protein 24 [Source:Xenbase;A...	[more]
arhgap24	5.047e-22	37.31	Rho GTPase activating protein 24 [Source:Xenbase;A...	[more]
arhgap24	5.050e-22	37.31	Rho GTPase activating protein 24 [Source:Xenbase;A...	[more]
arhgap24	1.767e-21	37.31	Rho GTPase activating protein 24 [Source:Xenbase;A...	[more]

BLAST of SMED30031277 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Dclk1	5.246e-23	38.06	doublecortin-like kinase 1 [Source:MGI Symbol;Acc:...	[more]
Dclk1	5.263e-23	38.06	doublecortin-like kinase 1 [Source:MGI Symbol;Acc:...	[more]
Dclk1	1.991e-22	38.06	doublecortin-like kinase 1 [Source:MGI Symbol;Acc:...	[more]
Dclk1	2.306e-22	38.06	doublecortin-like kinase 1 [Source:MGI Symbol;Acc:...	[more]
Dclk1	2.386e-22	38.06	doublecortin-like kinase 1 [Source:MGI Symbol;Acc:...	[more]

BLAST of SMED30031277 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|O15075\|DCLK1_HUMAN	3.084e-22	38.06	Serine/threonine-protein kinase DCLK1 OS=Homo sapi...	[more]
sp\|Q9JLM8\|DCLK1_MOUSE	3.681e-22	38.06	Serine/threonine-protein kinase DCLK1 OS=Mus muscu...	[more]
sp\|Q5MPA9\|DCLK2_RAT	1.472e-21	38.35	Serine/threonine-protein kinase DCLK2 OS=Rattus no...	[more]
sp\|O08875\|DCLK1_RAT	1.898e-21	38.06	Serine/threonine-protein kinase DCLK1 OS=Rattus no...	[more]
sp\|D2I3C6\|DCLK2_AILME	1.973e-21	38.35	Serine/threonine-protein kinase DCLK2 OS=Ailuropod...	[more]

BLAST of SMED30031277 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A3Q2P4H2	4.327e-25	40.71	Doublecortin-like kinase 2a OS=Fundulus heteroclit...	[more]
A0A2B4RRF8	6.804e-25	41.91	Serine/threonine-protein kinase DCLK1 OS=Stylophor...	[more]
A0A3M6UBT3	1.808e-24	41.18	Uncharacterized protein OS=Pocillopora damicornis ...	[more]
A7RVP7	4.383e-23	40.15	Predicted protein (Fragment) OS=Nematostella vecte...	[more]
A0A0P0CGZ3	8.118e-23	41.35	Doublecortin-like kinase OS=Holothuria glaberrima ...	[more]

BLAST of SMED30031277 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSAMXT00000039497.1	5.453e-23	39.55	pep primary_assembly:Astyanax_mexicanus-2.0:15:881...	[more]
ENSAMXT00000043405.1	2.008e-22	39.55	pep primary_assembly:Astyanax_mexicanus-2.0:15:881...	[more]
dclk2a	4.234e-22	38.06	doublecortin like kinase 2 [Source:NCBI gene;Acc:1...	[more]
dclk2a	4.251e-22	38.06	doublecortin like kinase 2 [Source:NCBI gene;Acc:1...	[more]
dclk2a	4.315e-22	38.06	doublecortin like kinase 2 [Source:NCBI gene;Acc:1...	[more]

BLAST of SMED30031277 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSPMAT00000010548.1	5.422e-20	33.33	pep scaffold:Pmarinus_7.0:GL477749:266235:284843:-...	[more]
ENSPMAT00000008023.1	6.969e-18	31.51	pep scaffold:Pmarinus_7.0:GL476334:542398:543161:1...	[more]
stk33	3.345e-17	35.97	serine/threonine kinase 33 [Source:ZFIN;Acc:ZDB-GE...	[more]
STK36	1.512e-16	33.58	serine/threonine kinase 36 [Source:HGNC Symbol;Acc...	[more]
ENSPMAT00000007114.1	4.176e-15	30.71	pep scaffold:Pmarinus_7.0:GL477366:299514:356138:-...	[more]

BLAST of SMED30031277 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5


Match Name	E-value	Identity	Description
DBF20	1.040e-16	30.71	Ser/Thr kinase involved in late nuclear division; ...	[more]
DBF2	5.037e-14	29.29	Ser/Thr kinase involved in transcription and stres...	[more]
CMK1	6.455e-13	28.89	Calmodulin-dependent protein kinase; may play a ro...	[more]
SNF1	8.646e-12	29.79	AMP-activated S/T protein kinase; forms a complex ...	[more]
PKH3	9.041e-12	28.03	Protein kinase with similarity to mammalian PDK1 a...	[more]

BLAST of SMED30031277 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO44488	6.061e-27	40.15	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO42432	5.686e-20	33.33	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO31543	1.127e-19	36.84	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO30768	3.053e-15	30.22	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO47961	1.380e-14	29.41	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30031277 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSORLT00000003850.2	3.265e-24	40.30	serine/threonine-protein kinase DCLK2 [Source:NCBI...	[more]
ENSORLT00000031333.1	3.265e-24	40.30	serine/threonine-protein kinase DCLK2 [Source:NCBI...	[more]
dclk1b	6.365e-24	36.84	serine/threonine-protein kinase DCLK1 [Source:NCBI...	[more]
dclk1b	6.387e-24	36.84	serine/threonine-protein kinase DCLK1 [Source:NCBI...	[more]
DCLK2	4.869e-23	37.41	doublecortin like kinase 2 [Source:NCBI gene;Acc:1...	[more]

BLAST of SMED30031277 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000040958.1	2.447e-93	99.28	SMESG000040958.1	[more]
SMESG000040958.1	1.656e-90	99.28	SMESG000040958.1	[more]
SMESG000078466.1	2.124e-21	34.56	SMESG000078466.1	[more]
SMESG000078466.1	2.235e-21	34.56	SMESG000078466.1	[more]
SMESG000078466.1	2.436e-21	34.56	SMESG000078466.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence

transcript sequence

>SMED30031277 ID=SMED30031277|Name=SMED30031277|organism=Schmidtea mediterranea sexual|type=transcript|length=442bp
TGTGATGAGAAATGGATTTTACCAATATATTTTCTAAACTTATATTTCGA
TGAATAATATTTTGAGATTGAAGAAACAAAAGAGCTGTACTCAGTTTAGA
AATAACACAGGACGTTTGAAGTTCAGATAGGACTCCGTTGGACTCCAACA
CAACTAGTAAATCACCACCCGGTGCCAATTCCATGACCAATATGACTTCC
TTCGGTGTTTCAAACTGATGCAACAAATGTGCAACGAATTCATGCCTACA
TCTTAATAACATATCAATTTCATTGTCTATTAAATATTCTCGTCCATCCA
ACAAGGCCTTGGAAACAATTTTCAACGCAACTTCTTCATTTTCTTCTTCG
GTGGCAGATTTTCCATAAAATACTCTACAGAAATCACCCTTTCCGATTTG
TGTTATTATTTGATATCTAAAAATATGATTTTATTTTTACTT

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000418	head
PLANA:0000420	parapharyngeal region

Vocabulary: **INTERPRO**
Term	Definition
IPR000719	Prot_kinase_dom
IPR011009	Kinase-like_dom_sf

Vocabulary: **molecular function**
Term	Definition
GO:0005524	ATP binding
GO:0004672	protein kinase activity

Vocabulary: **biological process**
Term	Definition
GO:0006468	protein phosphorylation

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 2..82 e-value: 4.2E-14 score: 52.5
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 1..87 score: 15.581
None	No IPR available	GENE3D	G3DSA:1.10.510.10		coord: 1..87 e-value: 3.4E-20 score: 74.4
None	No IPR available	PANTHER	PTHR24347	SERINE/THREONINE-PROTEIN KINASE	coord: 2..83
None	No IPR available	PANTHER	PTHR24347:SF354		coord: 2..83
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	SSF56112	Protein kinase-like (PK-like)	coord: 2..82

Main menu

SMED30031277

Zeng et. al., 2018

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Owlery

blazegraph-runner