alpha ketoglutarate dependent dioxygenase ABH4

Overview
Namealpha ketoglutarate dependent dioxygenase ABH4 (AFJ24779.1)
Smed IDSMED30031264
Length (bp)1350
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of alpha ketoglutarate dependent dioxygenase ABH4 (SMED30031264) t-SNE clustered cells

Violin plots show distribution of expression levels for alpha ketoglutarate dependent dioxygenase ABH4 (SMED30031264) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of alpha ketoglutarate dependent dioxygenase ABH4 (SMED30031264) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for alpha ketoglutarate dependent dioxygenase ABH4 (SMED30031264) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30031264

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
posterior sideSMED30031264h1SMcG0000347 dd_Smed_v4_4775_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30031264h1SMcG0000347 dd_Smed_v4_4775_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Human
Match: ALKBH4 (alkB homolog 4, lysine demethylase [Source:HGNC Symbol;Acc:HGNC:21900])

HSP 1 Score: 188.734 bits (478), Expect = 8.067e-56
Identity = 109/310 (35.16%), Postives = 166/310 (53.55%), Query Frame = 1 
Query:   28 ILAFVNYNNSIMKLCTCKGIRTCSSCNPNK-----IKIENQNCIVCYFCPKISKIVKENCISDLKCEEFHKVXXXXXXXXXXXXFLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSDIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFTPGFPDMF------DESSQAFFSSVCSENHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELSMEFTENDR--ELSRKLIDLSLTFNGQPV 918
            + A       +++ C CKGIRTC  C   +      ++         +C      V        +  +F        G++LIE+F+T +++  L+  +  + W  SQSGRRKQD+GPKVNF+K+K+    F GLP +    V R    P L+ F PVE CNL+Y P RG++IDPH DD WLWGERLV++N+ S T L+     P           + +A   SV + +   +     V + +PLP  SL++++G ARH+W HA+    +++ R+  T RELS EF    R  EL ++L+ ++L+F G+PV
Sbjct:    1 MAAAAAETPEVLRECGCKGIRTCLICERQRGSDPPWELPPAKTYRFIYCSDTGWAV------GTEESDFEGWAFPFPGVMLIEDFVTREEEAELVRLMDRDPWKLSQSGRRKQDYGPKVNFRKQKLKTEGFCGLPSFSREVVRRMGLYPGLEGFRPVEQCNLDYCPERGSAIDPHLDDAWLWGERLVSLNLLSPTVLSMCREAPGSLLLCSAPSAAPEALVDSVIAPSRSVLCQE--VEVAIPLPARSLLVLTGAARHQWKHAIHRRHIEARRVCVTFRELSAEFGPGGRQQELGQELLRIALSFQGRPV 302
[Close]
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Celegans
Match: nmad-1 (DNA N6-methyl adenine demethylase [Source:UniProtKB/Swiss-Prot;Acc:Q8MNT9])

HSP 1 Score: 142.51 bits (358), Expect = 6.747e-39
Identity = 69/200 (34.50%), Postives = 112/200 (56.00%), Query Frame = 1 
Query:  265 FLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFS--DIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFTPGFPDMFDESSQAFFSSVCSENHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELSMEFTEN 858
            FL+E +++ +L  I +  W  SQSGRRKQD+GPKVNFK +K+    F G+PEY +  +N+ S  D+ +L ++ P E+CNLEY   + ++I+ H DD W+WG RL++IN+ + + +T +                           +N +    V +P  SL+ ++ + R+ W H V A  ++  RIA T+RE + +F E 
Sbjct:   92 FLSESEESKILNMIDTVEWAQSQSGRRKQDYGPKVNFKHKKVKTDTFVGMPEYADMLLNKMSEYDVKKLGNYQPFEMCNLEYEEVKKSAIEMHQDDMWIWGNRLISINLINGSVMTLS--------------------------NDNKSFLCYVHMPHRSLLCMADECRYDWKHGVLAHHIRGRRIALTMREAAKDFAEG 265
[Close]
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Celegans
Match: nmad-1 (DNA N6-methyl adenine demethylase [Source:UniProtKB/Swiss-Prot;Acc:Q8MNT9])

HSP 1 Score: 114.39 bits (285), Expect = 2.139e-29
Identity = 48/105 (45.71%), Postives = 72/105 (68.57%), Query Frame = 1 
Query:  265 FLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFS--DIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLV 573
            FL+E +++ +L  I +  W  SQSGRRKQD+GPKVNFK +K+    F G+PEY +  +N+ S  D+ +L ++ P E+CNLEY   + ++I+ H DD W+WG RL+
Sbjct:   92 FLSESEESKILNMIDTVEWAQSQSGRRKQDYGPKVNFKHKKVKTDTFVGMPEYADMLLNKMSEYDVKKLGNYQPFEMCNLEYEEVKKSAIEMHQDDMWIWGNRLI 196
[Close]
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Fly
Match: CG4036 (gene:FBgn0032149 transcript:FBtr0303447)

HSP 1 Score: 162.54 bits (410), Expect = 3.438e-46
Identity = 99/277 (35.74%), Postives = 144/277 (51.99%), Query Frame = 1 
Query:   61 MKLCTCKGIRTCSSCNPN----KIKIENQ-----NCIVCYFCPKISKIVKENCISDLKCEEFHKVXXXXXX--XXXXXXFLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSDIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFTPGFPDMFDESSQAFFSSVCSE------NHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELS 840
            ++ C CKG+RTC SC  +    K  +  Q         C  C  + +    N +   K  E HK +  L    I++ E FL+ D+   L+  +    W  SQSGRRKQ+FGPK NFKKRK+ L  F G P   E    RF D+P L+ F  +E C+LEY P++GASIDPH DD W+WGER+VT+N    + LT TP       + +    +S   E        + +       +++P+P  SL+++ G AR+++ H+V   DVQ  R+    RE +
Sbjct:    4 IRPCGCKGVRTCLSCEQDFHIAKTSLREQFQQLEAWSYCIQCDLLQRGWDTNHVQ--KDHENHKKDEGLPLPGILVQEEFLSVDEGAQLIADLDDLPWDISQSGRRKQNFGPKTNFKKRKLRLGSFAGFPRTTEYVQRRFEDVPLLRGFQTIEQCSLEYEPSKGASIDPHVDDCWIWGERVVTVNCLGDSVLTLTPYEVQQSGKYNLDLVASYEDELLAPLLTDDQLATFEGKVLRIPMPNLSLIVLYGPARYQFEHSVLREDVQERRVCVAYREFT 278
[Close]
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Fly
Match: CG4036 (gene:FBgn0032149 transcript:FBtr0079870)

HSP 1 Score: 162.54 bits (410), Expect = 3.438e-46
Identity = 99/277 (35.74%), Postives = 144/277 (51.99%), Query Frame = 1 
Query:   61 MKLCTCKGIRTCSSCNPN----KIKIENQ-----NCIVCYFCPKISKIVKENCISDLKCEEFHKVXXXXXX--XXXXXXFLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSDIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFTPGFPDMFDESSQAFFSSVCSE------NHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELS 840
            ++ C CKG+RTC SC  +    K  +  Q         C  C  + +    N +   K  E HK +  L    I++ E FL+ D+   L+  +    W  SQSGRRKQ+FGPK NFKKRK+ L  F G P   E    RF D+P L+ F  +E C+LEY P++GASIDPH DD W+WGER+VT+N    + LT TP       + +    +S   E        + +       +++P+P  SL+++ G AR+++ H+V   DVQ  R+    RE +
Sbjct:    4 IRPCGCKGVRTCLSCEQDFHIAKTSLREQFQQLEAWSYCIQCDLLQRGWDTNHVQ--KDHENHKKDEGLPLPGILVQEEFLSVDEGAQLIADLDDLPWDISQSGRRKQNFGPKTNFKKRKLRLGSFAGFPRTTEYVQRRFEDVPLLRGFQTIEQCSLEYEPSKGASIDPHVDDCWIWGERVVTVNCLGDSVLTLTPYEVQQSGKYNLDLVASYEDELLAPLLTDDQLATFEGKVLRIPMPNLSLIVLYGPARYQFEHSVLREDVQERRVCVAYREFT 278
[Close]
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Zebrafish
Match: alkbh4 (alkB homolog 4, lysine demthylase [Source:ZFIN;Acc:ZDB-GENE-060526-275])

HSP 1 Score: 218.394 bits (555), Expect = 1.122e-67
Identity = 119/291 (40.89%), Postives = 168/291 (57.73%), Query Frame = 1 
Query:   70 CTCKGIRTCSSCNPNKIK-IENQNCIVCY---FCPKISKIVKENCISDLKCEEFHKVXXXXXXXXXXXXFLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSDIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFT--PGFPDMFDESSQAFFSSVCSENHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELSMEFTENDRE--LSRKLIDLSLTFNGQPV 918
            C CKGIRTC  C  ++ K +  +N ++ Y   + P +   V+E   S  +C EF  V +  N       F++ED++  L+  +  + W +SQSGRRKQDFGPKVNFKKR++++  F GLP    R + R SD+P+L  F PVE CNL+Y+  RG++IDPH DD WLWGE LVT+N+ S T LT +   G+ DM                         V + V LP  SLV++ GDARH+W HA+   D+   R+ ST RELS EF    ++  L  +L+D++L+F G P+
Sbjct:   22 CGCKGIRTCLRCETDETKHLLQKNDLIHYDFIYDPVLKSAVREEEGSTPQCFEFPGVLLWEN-------FVSEDEERELVSRMDQDVWRESQSGRRKQDFGPKVNFKKRRVHVGSFSGLPAISRRLLVRMSDLPQLSSFKPVEQCNLDYDSLRGSAIDPHLDDSWLWGENLVTVNLLSDTVLTLSLDQGWGDM---------------------EQGEVRVAVRLPRRSLVVLYGDARHRWKHAIHRKDIHGRRVCSTFRELSAEFLPGGQQEKLGSELLDIALSFQGAPL 284
[Close]
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Zebrafish
Match: alkbh4 (alkB homolog 4, lysine demthylase [Source:ZFIN;Acc:ZDB-GENE-060526-275])

HSP 1 Score: 191.815 bits (486), Expect = 1.081e-58
Identity = 94/204 (46.08%), Postives = 125/204 (61.27%), Query Frame = 1 
Query:  319 WVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSDIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFT--PGFPDMFDESSQAFFSSVCSENHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELSMEFTENDRE--LSRKLIDLSLTFNGQPV 918
            W +SQSGRRKQDFGPKVNFKKR++++  F GLP    R + R SD+P+L  F PVE CNL+Y+  RG++IDPH DD WLWGE LVT+N+ S T LT +   G+ DM                         V + V LP  SLV++ GDARH+W HA+   D+   R+ ST RELS EF    ++  L  +L+D++L+F G P+
Sbjct:    6 WRESQSGRRKQDFGPKVNFKKRRVHVGSFSGLPAISRRLLVRMSDLPQLSSFKPVEQCNLDYDSLRGSAIDPHLDDSWLWGENLVTVNLLSDTVLTLSLDQGWGDM---------------------EQGEVRVAVRLPRRSLVVLYGDARHRWKHAIHRKDIHGRRVCSTFRELSAEFLPGGQQEKLGSELLDIALSFQGAPL 188
[Close]
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Xenopus
Match: cisd2 (CDGSH iron sulfur domain 2 [Source:Xenbase;Acc:XB-GENE-5969737])

HSP 1 Score: 183.726 bits (465), Expect = 6.921e-54
Identity = 114/309 (36.89%), Postives = 165/309 (53.40%), Query Frame = 1 
Query:   64 KLCTCKGIRTCSSCNPNKIKI-ENQNCIVCYFCPKISKIVKENCISDLKCEEFHKVXXXXXXXXXXXXFLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSDIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFTPG------FPDMFDESSQAFFSSVCSENHENMGNN---------------CAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELSMEFTENDR--ELSRKLIDLSLTFNGQPV 918
            K C CKGIR+C  C        +  +  + +    +  I K      L    FH       G+ LI++F+TED++ +L+  +    W  SQSGRRKQDFGPKVNFKK+K+ +  F GLP   +    R      L+ F PVE CNL+Y+  RG+SIDPHFDD WLWGERLV++N+ S T  T T           +  E S+   +S    N  +M +                C V + + LP  SLV++ GDAR+KW HA+   +++  R+ ST RELS EF+   +  +L   L++++L+F G+P+
Sbjct:   10 KGCGCKGIRSCLLCERGATGAPKGLHKKLPFIYSPLEGIAK-----GLDGCWFH-----FPGVYLIQDFVTEDEEQHLVNFMDQEEWRLSQSGRRKQDFGPKVNFKKQKLRVGNFSGLPNVSQVIWERMKQHTVLEGFLPVEQCNLDYHSERGSSIDPHFDDSWLWGERLVSLNLLSDTVFTMTSDDVSSLQLMPVLQEGSELNSTSSDGNNASDMNHTQNPGYSKSQAKHVPYCDVEVAIRLPRRSLVVLYGDARYKWKHAIHRENIEHRRVCSTFRELSKEFSLGGKKEKLGEMLVNIALSFQGKPI 308
[Close]
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Mouse
Match: Alkbh4 (alkB homolog 4, lysine demethylase [Source:MGI Symbol;Acc:MGI:1919291])

HSP 1 Score: 202.986 bits (515), Expect = 2.035e-61
Identity = 116/311 (37.30%), Postives = 181/311 (58.20%), Query Frame = 1 
Query:   55 SIMKLCTCKGIRTCSSCN-------PNKIKIENQNCIVCYFCPKISKIVKENCISDLKCEEFHKVXXXXXXXXXXXXFLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSDIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFTPGF-------------PDMFDESSQAFFSSV-CSENHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELSMEFTENDR--ELSRKLIDLSLTFNGQPV 918
            S+++ C CKGIRTC  C        P +I ++ + C +  +CP           SDL+   F        G+ LI++F+T +++  ++  +  + W  SQSGR+KQD+GPKVNF+K+K+ +  FQGLP + ++ V R    P L+DF PVE CNL+Y+P RG++IDPH DD WLWGERLV++N+ S+T ++ +P               PD F++S  A   SV C E          V + + +P  SL++++G ARH+W HA+    +++ R+ +T RELS EF    +  EL ++L+  +L+F G+PV
Sbjct:    8 SLLQECGCKGIRTCLICERQRHRDPPWQICLQKKCCFL--YCPDTGWAAGAEG-SDLEGWAF-----PFPGVTLIQDFVTPEEEAEMVRLMDCDPWKLSQSGRKKQDYGPKVNFRKQKLKMAGFQGLPGFSQKVVQRMGLYPGLEDFQPVEQCNLDYSPERGSAIDPHLDDAWLWGERLVSLNLLSATVVSMSPEAPGSLLLCSAPSVRPDAFEDSLVAPSRSVPCQE----------VEVAITVPRRSLLVLTGAARHQWTHAIHRRHIKARRVCATFRELSSEFLPGGKQQELGQELLQAALSFQGRPV 300
[Close]
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Mouse
Match: Alkbh4 (alkB homolog 4, lysine demethylase [Source:MGI Symbol;Acc:MGI:1919291])

HSP 1 Score: 188.348 bits (477), Expect = 6.495e-57
Identity = 92/218 (42.20%), Postives = 137/218 (62.84%), Query Frame = 1 
Query:  313 NSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSDIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFTPGF-------------PDMFDESSQAFFSSV-CSENHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELSMEFTENDR--ELSRKLIDLSLTFNGQPV 918
            + W  SQSGR+KQD+GPKVNF+K+K+ +  FQGLP + ++ V R    P L+DF PVE CNL+Y+P RG++IDPH DD WLWGERLV++N+ S+T ++ +P               PD F++S  A   SV C E          V + + +P  SL++++G ARH+W HA+    +++ R+ +T RELS EF    +  EL ++L+  +L+F G+PV
Sbjct:    8 DPWKLSQSGRKKQDYGPKVNFRKQKLKMAGFQGLPGFSQKVVQRMGLYPGLEDFQPVEQCNLDYSPERGSAIDPHLDDAWLWGERLVSLNLLSATVVSMSPEAPGSLLLCSAPSVRPDAFEDSLVAPSRSVPCQE----------VEVAITVPRRSLLVLTGAARHQWTHAIHRRHIKARRVCATFRELSSEFLPGGKQQELGQELLQAALSFQGRPV 215
[Close]
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Mouse
Match: Alkbh4 (alkB homolog 4, lysine demethylase [Source:MGI Symbol;Acc:MGI:1919291])

HSP 1 Score: 138.658 bits (348), Expect = 4.421e-39
Identity = 57/104 (54.81%), Postives = 79/104 (75.96%), Query Frame = 1 
Query:  313 NSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSDIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFTPGFP 624
            + W  SQSGR+KQD+GPKVNF+K+K+ +  FQGLP + ++ V R    P L+DF PVE CNL+Y+P RG++IDPH DD WLWGERLV++N+ S+T ++ +P  P
Sbjct:    8 DPWKLSQSGRKKQDYGPKVNFRKQKLKMAGFQGLPGFSQKVVQRMGLYPGLEDFQPVEQCNLDYSPERGSAIDPHLDDAWLWGERLVSLNLLSATVVSMSPEAP 111
[Close]
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. UniProt/SwissProt
Match: sp|Q9D8F1|ALKB4_MOUSE (Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4 OS=Mus musculus OX=10090 GN=Alkbh4 PE=1 SV=1)

HSP 1 Score: 202.986 bits (515), Expect = 1.424e-60
Identity = 116/311 (37.30%), Postives = 181/311 (58.20%), Query Frame = 1 
Query:   55 SIMKLCTCKGIRTCSSCN-------PNKIKIENQNCIVCYFCPKISKIVKENCISDLKCEEFHKVXXXXXXXXXXXXFLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSDIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFTPGF-------------PDMFDESSQAFFSSV-CSENHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELSMEFTENDR--ELSRKLIDLSLTFNGQPV 918
            S+++ C CKGIRTC  C        P +I ++ + C +  +CP           SDL+   F        G+ LI++F+T +++  ++  +  + W  SQSGR+KQD+GPKVNF+K+K+ +  FQGLP + ++ V R    P L+DF PVE CNL+Y+P RG++IDPH DD WLWGERLV++N+ S+T ++ +P               PD F++S  A   SV C E          V + + +P  SL++++G ARH+W HA+    +++ R+ +T RELS EF    +  EL ++L+  +L+F G+PV
Sbjct:    8 SLLQECGCKGIRTCLICERQRHRDPPWQICLQKKCCFL--YCPDTGWAAGAEG-SDLEGWAF-----PFPGVTLIQDFVTPEEEAEMVRLMDCDPWKLSQSGRKKQDYGPKVNFRKQKLKMAGFQGLPGFSQKVVQRMGLYPGLEDFQPVEQCNLDYSPERGSAIDPHLDDAWLWGERLVSLNLLSATVVSMSPEAPGSLLLCSAPSVRPDAFEDSLVAPSRSVPCQE----------VEVAITVPRRSLLVLTGAARHQWTHAIHRRHIKARRVCATFRELSSEFLPGGKQQELGQELLQAALSFQGRPV 300
[Close]
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. UniProt/SwissProt
Match: sp|Q9NXW9|ALKB4_HUMAN (Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4 OS=Homo sapiens OX=9606 GN=ALKBH4 PE=1 SV=1)

HSP 1 Score: 188.734 bits (478), Expect = 3.874e-55
Identity = 109/310 (35.16%), Postives = 166/310 (53.55%), Query Frame = 1 
Query:   28 ILAFVNYNNSIMKLCTCKGIRTCSSCNPNK-----IKIENQNCIVCYFCPKISKIVKENCISDLKCEEFHKVXXXXXXXXXXXXFLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSDIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFTPGFPDMF------DESSQAFFSSVCSENHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELSMEFTENDR--ELSRKLIDLSLTFNGQPV 918
            + A       +++ C CKGIRTC  C   +      ++         +C      V        +  +F        G++LIE+F+T +++  L+  +  + W  SQSGRRKQD+GPKVNF+K+K+    F GLP +    V R    P L+ F PVE CNL+Y P RG++IDPH DD WLWGERLV++N+ S T L+     P           + +A   SV + +   +     V + +PLP  SL++++G ARH+W HA+    +++ R+  T RELS EF    R  EL ++L+ ++L+F G+PV
Sbjct:    1 MAAAAAETPEVLRECGCKGIRTCLICERQRGSDPPWELPPAKTYRFIYCSDTGWAV------GTEESDFEGWAFPFPGVMLIEDFVTREEEAELVRLMDRDPWKLSQSGRRKQDYGPKVNFRKQKLKTEGFCGLPSFSREVVRRMGLYPGLEGFRPVEQCNLDYCPERGSAIDPHLDDAWLWGERLVSLNLLSPTVLSMCREAPGSLLLCSAPSAAPEALVDSVIAPSRSVLCQE--VEVAIPLPARSLLVLTGAARHQWKHAIHRRHIEARRVCVTFRELSAEFGPGGRQQELGQELLRIALSFQGRPV 302
[Close]
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. UniProt/SwissProt
Match: sp|Q8MNT9|NMAD1_CAEEL (DNA N6-methyl adenine demethylase OS=Caenorhabditis elegans OX=6239 GN=nmad-1 PE=1 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 8.583e-38
Identity = 69/200 (34.50%), Postives = 112/200 (56.00%), Query Frame = 1 
Query:  265 FLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFS--DIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFTPGFPDMFDESSQAFFSSVCSENHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELSMEFTEN 858
            FL+E +++ +L  I +  W  SQSGRRKQD+GPKVNFK +K+    F G+PEY +  +N+ S  D+ +L ++ P E+CNLEY   + ++I+ H DD W+WG RL++IN+ + + +T +                           +N +    V +P  SL+ ++ + R+ W H V A  ++  RIA T+RE + +F E 
Sbjct:   92 FLSESEESKILNMIDTVEWAQSQSGRRKQDYGPKVNFKHKKVKTDTFVGMPEYADMLLNKMSEYDVKKLGNYQPFEMCNLEYEEVKKSAIEMHQDDMWIWGNRLISINLINGSVMTLS--------------------------NDNKSFLCYVHMPHRSLLCMADECRYDWKHGVLAHHIRGRRIALTMREAAKDFAEG 265
[Close]
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. TrEMBL
Match: I1ZIC5 (Alpha ketoglutarate dependent dioxygenase ABH4 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 566.229 bits (1458), Expect = 0.000e+0
Identity = 287/287 (100.00%), Postives = 287/287 (100.00%), Query Frame = 1 
Query:   61 MKLCTCKGIRTCSSCNPNKIKIENQNCIVCYFCPKISKIVKENCISDLKCEEFHKVXXXXXXXXXXXXFLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSDIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFTPGFPDMFDESSQAFFSSVCSENHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELSMEFTENDRELSRKLIDLSLTFNGQPVN 921
            MKLCTCKGIRTCSSCNPNKIKIENQNCIVCYFCPKISKIVKENCISDLKCEEFHKVNIELNGIILIENFLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSDIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFTPGFPDMFDESSQAFFSSVCSENHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELSMEFTENDRELSRKLIDLSLTFNGQPVN
Sbjct:    1 MKLCTCKGIRTCSSCNPNKIKIENQNCIVCYFCPKISKIVKENCISDLKCEEFHKVNIELNGIILIENFLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSDIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFTPGFPDMFDESSQAFFSSVCSENHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELSMEFTENDRELSRKLIDLSLTFNGQPVN 287
[Close]
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. TrEMBL
Match: F1QMZ8 (AlkB homolog 4, lysine demthylase (Fragment) OS=Danio rerio OX=7955 GN=alkbh4 PE=4 SV=1)

HSP 1 Score: 218.394 bits (555), Expect = 2.511e-64
Identity = 119/291 (40.89%), Postives = 168/291 (57.73%), Query Frame = 1 
Query:   70 CTCKGIRTCSSCNPNKIK-IENQNCIVCY---FCPKISKIVKENCISDLKCEEFHKVXXXXXXXXXXXXFLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSDIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFT--PGFPDMFDESSQAFFSSVCSENHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELSMEFTENDRE--LSRKLIDLSLTFNGQPV 918
            C CKGIRTC  C  ++ K +  +N ++ Y   + P +   V+E   S  +C EF  V +  N       F++ED++  L+  +  + W +SQSGRRKQDFGPKVNFKKR++++  F GLP    R + R SD+P+L  F PVE CNL+Y+  RG++IDPH DD WLWGE LVT+N+ S T LT +   G+ DM                         V + V LP  SLV++ GDARH+W HA+   D+   R+ ST RELS EF    ++  L  +L+D++L+F G P+
Sbjct:   22 CGCKGIRTCLRCETDETKHLLQKNDLIHYDFIYDPVLKSAVREEEGSTPQCFEFPGVLLWEN-------FVSEDEERELVSRMDQDVWRESQSGRRKQDFGPKVNFKKRRVHVGSFSGLPAISRRLLVRMSDLPQLSSFKPVEQCNLDYDSLRGSAIDPHLDDSWLWGENLVTVNLLSDTVLTLSLDQGWGDM---------------------EQGEVRVAVRLPRRSLVVLYGDARHRWKHAIHRKDIHGRRVCSTFRELSAEFLPGGQQEKLGSELLDIALSFQGAPL 284
[Close]
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. TrEMBL
Match: Q502B6 (LOC553475 protein (Fragment) OS=Danio rerio OX=7955 GN=alkbh4 PE=2 SV=1)

HSP 1 Score: 216.853 bits (551), Expect = 9.291e-64
Identity = 119/292 (40.75%), Postives = 166/292 (56.85%), Query Frame = 1 
Query:   70 CTCKGIRTCSSCNPNKIK--IENQNCIVCY---FCPKISKIVKENCISDLKCEEFHKVXXXXXXXXXXXXFLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSDIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFT--PGFPDMFDESSQAFFSSVCSENHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELSMEFTENDRE--LSRKLIDLSLTFNGQPV 918
            C CKGIRTC  C  +  K  +   N ++ Y   + P +   V+E   S  +C EF  V +  N       F++ED++  L+  +  + W +SQSGRRKQDFGPKVNFKKR++++  F GLP    R + R SD+P+L  F PVE CNL+Y+  RG++IDPH DD WLWGE LVT+N+ S T LT +   G+ DM                         V + V LP  SLV++ GDARH+W HA+   D+   R+ ST RELS EF    ++  L  +L+D++L+F G P+
Sbjct:   13 CGCKGIRTCLRCETDGTKQHLLQNNELIHYDFIYDPVLKSAVREEEGSTPQCFEFPGVLLWEN-------FVSEDEERELVSRMDQDVWRESQSGRRKQDFGPKVNFKKRRVHVGSFSGLPAISRRLLVRMSDLPQLSSFKPVEQCNLDYDSLRGSAIDPHLDDSWLWGENLVTVNLLSDTVLTLSLDQGWGDM---------------------EQGEVRVAVRLPRRSLVVLYGDARHRWKHAIHRKDIHGRRVCSTFRELSAEFLPGGQQEKLGSELLDIALSFQGAPL 276
[Close]
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. TrEMBL
Match: V4AYG2 (Fe2OG dioxygenase domain-containing protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_142409 PE=4 SV=1)

HSP 1 Score: 211.075 bits (536), Expect = 1.314e-61
Identity = 126/296 (42.57%), Postives = 164/296 (55.41%), Query Frame = 1 
Query:   64 KLCTCKGIRTCSSCNPNKIKIEN----QNCIVCYFCPKISKI----VKENCISDLKCEEFHKVXXXXXXXXXXXXFLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSDIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFTPGFPDMFDESSQAFFSSVCSENHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELSMEFTENDR--ELSRKLIDLSLTFNGQPVN 921
            K C CKGIR+C  C  NK KIEN    +  +   +C    K        N   D +  +F  + +          F+ E  +  L+  I    + DSQSGRRKQD+GPKVNFKK+K+    F GLP+Y E      + +P LKDF PVELCNLEY P RG++IDPH+DDFWLWGERLVTIN+ S T L F      + D+ S                    + ++V LP  SLV+V G+ARHKW H +   D+Q+ RIA T RELS EF ++ +  E+  KL D++LTFNG  V 
Sbjct:    5 KSCGCKGIRSCLLCEDNKDKIENVSEKKEKVTLKYCDLCKKAWAWGFHPNHTGDSR--DFEGIYLHEE-------FIDEILEAELIDEIDKTVYSDSQSGRRKQDYGPKVNFKKQKVKYEVFTGLPKYSEVLYTTLTALPILKDFIPVELCNLEYIPERGSAIDPHYDDFWLWGERLVTINLISETVLYF------INDQDSN-------------------IEVQVTLPPRSLVVVFGEARHKWKHGIHRDDIQNRRIAMTFRELSQEFEKDGKSSEIGEKLKDIALTFNGTAVG 266
[Close]
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. TrEMBL
Match: A0A4S2MC89 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_001077 PE=4 SV=1)

HSP 1 Score: 212.231 bits (539), Expect = 1.536e-61
Identity = 127/307 (41.37%), Postives = 172/307 (56.03%), Query Frame = 1 
Query:   67 LCTCKGIRTCSSCNPNKI-------KIENQNCIVCYFCPKISKIVKENCISDLKCEEF--HKVXXXXXXXXXXXXFLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSDI----PELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFTPGFPDMFDESSQAFFSSVCSE--NHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELSMEFTEN-----------DRELSRKLIDLSLTFNG 909
            LC CKGIR C  C PNK        + E+  C  C  C + S      C     C EF  H +++  N       F+TE+++ +LL  I  N W  SQSGRRKQD+GPKVNFK++++    F GLP Y    V+R +      P  + F PVELCNLEY P RGASI PH DD WLWG+RLVT+++ SST L FT   P+       A F  + S+      + ++  V+++VPLP  SLVIV G AR+ W H++ +AD+QS RIA T RELS +F              D+ + R+L+ ++ TF+G
Sbjct:   10 LCGCKGIRKCKVCQPNKPLTYENLPRFESTFCSECALCYQGS----HRCHGSPACGEFTFHGISVVSN-------FVTEEEEAWLLDQIDQNPWALSQSGRRKQDYGPKVNFKRQRVVPGCFTGLPSYSRFLVDRINQTIRTSPVRQTFIPVELCNLEYCPERGASIVPHLDDVWLWGDRLVTLSLCSSTTLIFT--LPNSGSTEVLAEFERIRSDLGQQAEITSSSDVAVRVPLPPRSLVIVVGPARYVWHHSIRSADIQSRRIAMTFRELSKQFVPQTSISEPEQCSEDQLVGRRLLQIAQTFHG 303
[Close]
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Cavefish
Match: alkbh4 (alkB homolog 4, lysine demethylase [Source:NCBI gene;Acc:103026086])

HSP 1 Score: 207.608 bits (527), Expect = 1.079e-63
Identity = 120/296 (40.54%), Postives = 169/296 (57.09%), Query Frame = 1 
Query:   61 MKLCTCKGIRTCSSC--NPNKIKIENQNCIVCY---FCPKISKIVKENCISDLKCEEFHKVXXXXXXXXXXXXFLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSDIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFTPG--FPDMFDESSQAFFSSVCSENHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELSMEFTENDRE--LSRKLIDLSLTFNGQPVN 921
            + +C CKGIRTC  C     K ++++Q   + Y   + P+ SK+      S L+   F  V +  N       F++ED++  L+  +  + W +SQSGRRKQDFGPKVNFKK++I L  F GLP    + V+R +  P L  F PVE CNL+Y+P RG++IDPH DD WLWGERLVTIN+ S T LT +    + DM                     +   V + V LP   L+++ GDARH W HA+   D+QS R+ ST RELS EF E  ++  L   L+D+++++NG P+N
Sbjct:    7 VAVCGCKGIRTCLICEGKDGKHQLQHQTHTIQYDFRYDPE-SKLAVPAGSSVLESFPFPGVFLWEN-------FVSEDEETELVEKMDQDIWRESQSGRRKQDFGPKVNFKKQRIRLGGFNGLPALSHKLVDRMTKEPLLSGFRPVEQCNLDYDPKRGSAIDPHLDDSWLWGERLVTINLLSDTVLTMSLDKCWGDM---------------------DQGEVRVAVNLPRRCLIVLYGDARHHWKHAIHRKDIQSRRVCSTFRELSAEFLEGGKQEALGLGLLDVAMSYNGVPIN 273
[Close]
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Nematostella
Match: EDO47562 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RM95])

HSP 1 Score: 199.134 bits (505), Expect = 4.998e-61
Identity = 122/291 (41.92%), Postives = 163/291 (56.01%), Query Frame = 1 
Query:   70 CTCKGIRTCSSCNPNKIKIENQNCI-----VCYFCPKISKIVKENCISDLKCEEFHKVXXXXXXXXXXXXFLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSD-IPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFTPGFPDMFDESSQAFFSSVCSENHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELSMEFTENDR--ELSRKLIDLSLTFNGQPV 918
            C C GIR+C  C  N    ++   +        FC   S+ +        +  +  +    L+GI LIE+F+++ ++  ++  I    W  SQSGRRKQD+GP+VNFKK+K+ ++ F GLP + E  V R +D +P LKDF PVELCNLEY+  RG+SID HFDDFWLWGERLVT+N+ S+T LT T    D ++                         I VP+P  SL+IVSG ARH W HAV   D+   RIA TLRELS EF +  R   +  + I  +LTFNG  V
Sbjct:    5 CGCTGIRSCLFCKDNTKTSQSTTSVDEAKTKYLFCHLCSQTLPLGGTCSHELGDCGRYGPSLDGITLIEDFVSQREEARIVQVIDETVWKPSQSGRRKQDYGPQVNFKKKKVKMSHFNGLPAFSEFLVRRMNDDVPGLKDFVPVELCNLEYDEARGSSIDAHFDDFWLWGERLVTLNLLSATRLTMT---KDTYE-------------------------ISVPMPRRSLIIVSGAARHLWQHAVKREDISGRRIAITLRELSEEFCKGGRNENVGFQAIKTALTFNGTSV 267
[Close]
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Medaka
Match: alkbh4 (alkB homolog 4, lysine demethylase [Source:NCBI gene;Acc:101172431])

HSP 1 Score: 200.29 bits (508), Expect = 7.086e-61
Identity = 117/288 (40.62%), Postives = 160/288 (55.56%), Query Frame = 1 
Query:   70 CTCKGIRTCSSCNPNKIKIENQNCIVCYFCPKISKIVKENCISDLK-CEEFHKVXXXXXXXXXXXXFLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSDIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFT--PGFPDMFDESSQAFFSSVCSENHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELSMEFTENDR--ELSRKLIDLSLTFNGQPV 918
            C CKGIR C  C   K K   ++       PK +     N  + L  C++    +    GI L ++F++E+++  L+  +  ++W  SQSGRRKQDFGPKVNFKKRK+ +  F GLP   +  V R    P L  F PVE CNL+YNP RG++IDPHFDD WLWGERLVTIN+ S T LT +   G P+                    +G +  + + V LP   LV+V G+ARH+W HA+   D+   R+ ST RELS EF    +  EL  +L+ ++L F G P+
Sbjct:   13 CGCKGIRLCLLCENRKEKEGTKD-------PKAAHHFIYNPETKLAVCKDAGSASFSFPGIFLWDDFISEEEEKQLINSMDQDAWNPSQSGRRKQDFGPKVNFKKRKVRVGGFSGLPSLSKELVLRMQQEPCLSGFQPVEQCNLDYNPQRGSAIDPHFDDSWLWGERLVTINMLSDTTLTMSLEQGLPE--------------------LGLSEEIHVAVRLPRRCLVVVYGEARHRWKHAIRRQDIHKRRVCSTYRELSAEFLPGGQQAELGAQLLKIALDFKGIPI 273
[Close]
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Planmine SMEST
Match: SMESG000011161.1 (SMESG000011161.1)

HSP 1 Score: 565.459 bits (1456), Expect = 0.000e+0
Identity = 286/287 (99.65%), Postives = 286/287 (99.65%), Query Frame = 1 
Query:   61 MKLCTCKGIRTCSSCNPNKIKIENQNCIVCYFCPKISKIVKENCISDLKCEEFHKVXXXXXXXXXXXXFLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSDIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFTPGFPDMFDESSQAFFSSVCSENHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELSMEFTENDRELSRKLIDLSLTFNGQPVN 921
            MKLCTCKGIRTCSSCNPNKIKIENQNCI CYFCPKISKIVKENCISDLKCEEFHKVNIELNGIILIENFLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSDIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFTPGFPDMFDESSQAFFSSVCSENHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELSMEFTENDRELSRKLIDLSLTFNGQPVN
Sbjct:    1 MKLCTCKGIRTCSSCNPNKIKIENQNCIACYFCPKISKIVKENCISDLKCEEFHKVNIELNGIILIENFLTEDDKNYLLGGICSNSWVDSQSGRRKQDFGPKVNFKKRKINLTKFQGLPEYIERFVNRFSDIPELKDFNPVELCNLEYNPTRGASIDPHFDDFWLWGERLVTINVQSSTYLTFTPGFPDMFDESSQAFFSSVCSENHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQSTRIASTLRELSMEFTENDRELSRKLIDLSLTFNGQPVN 287
The following BLAST results are available for this feature:
BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ALKBH48.067e-5635.16alkB homolog 4, lysine demethylase [Source:HGNC Sy... [more]
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BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
nmad-16.747e-3934.50DNA N6-methyl adenine demethylase [Source:UniProt... [more]
nmad-12.139e-2945.71DNA N6-methyl adenine demethylase [Source:UniProt... [more]
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BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CG40363.438e-4635.74gene:FBgn0032149 transcript:FBtr0303447[more]
CG40363.438e-4635.74gene:FBgn0032149 transcript:FBtr0079870[more]
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BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
alkbh41.122e-6740.89alkB homolog 4, lysine demthylase [Source:ZFIN;Acc... [more]
alkbh41.081e-5846.08alkB homolog 4, lysine demthylase [Source:ZFIN;Acc... [more]
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BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
cisd26.921e-5436.89CDGSH iron sulfur domain 2 [Source:Xenbase;Acc:XB-... [more]
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BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Alkbh42.035e-6137.30alkB homolog 4, lysine demethylase [Source:MGI Sym... [more]
Alkbh46.495e-5742.20alkB homolog 4, lysine demethylase [Source:MGI Sym... [more]
Alkbh44.421e-3954.81alkB homolog 4, lysine demethylase [Source:MGI Sym... [more]
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BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|Q9D8F1|ALKB4_MOUSE1.424e-6037.30Alpha-ketoglutarate-dependent dioxygenase alkB hom... [more]
sp|Q9NXW9|ALKB4_HUMAN3.874e-5535.16Alpha-ketoglutarate-dependent dioxygenase alkB hom... [more]
sp|Q8MNT9|NMAD1_CAEEL8.583e-3834.50DNA N6-methyl adenine demethylase OS=Caenorhabditi... [more]
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BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
I1ZIC50.000e+0100.00Alpha ketoglutarate dependent dioxygenase ABH4 OS=... [more]
F1QMZ82.511e-6440.89AlkB homolog 4, lysine demthylase (Fragment) OS=Da... [more]
Q502B69.291e-6440.75LOC553475 protein (Fragment) OS=Danio rerio OX=795... [more]
V4AYG21.314e-6142.57Fe2OG dioxygenase domain-containing protein OS=Lot... [more]
A0A4S2MC891.536e-6141.37Uncharacterized protein OS=Opisthorchis felineus O... [more]
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BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
alkbh41.079e-6340.54alkB homolog 4, lysine demethylase [Source:NCBI ge... [more]
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BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO475624.998e-6141.92Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
alkbh47.086e-6140.63alkB homolog 4, lysine demethylase [Source:NCBI ge... [more]
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BLAST of alpha ketoglutarate dependent dioxygenase ABH4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000011161.10.000e+099.65SMESG000011161.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30031264
GenBankAFJ24779.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031264 ID=SMED30031264|Name=alpha ketoglutarate dependent dioxygenase ABH4|organism=Schmidtea mediterranea sexual|type=transcript|length=1350bp
TTTGAAATGAATTTGTTTAAATAATATATTCTTGCTTTTGTTAATTACAA
TAATTCAATTATGAAACTTTGTACATGCAAAGGTATAAGAACATGTTCTT
CGTGTAATCCAAATAAAATCAAAATCGAAAATCAAAATTGCATAGTTTGT
TATTTCTGTCCAAAAATATCTAAAATAGTTAAAGAAAACTGCATTTCTGA
TCTTAAATGTGAAGAGTTTCATAAGGTTAATATTGAATTAAATGGAATTA
TTCTAATAGAAAACTTTTTGACTGAAGATGATAAAAATTATTTATTAGGT
GGAATATGTAGTAATTCATGGGTAGATTCTCAATCTGGTCGTAGGAAACA
GGATTTTGGACCGAAAGTTAACTTTAAAAAGCGTAAAATAAATCTGACAA
AATTTCAAGGTTTACCAGAATATATTGAAAGATTTGTTAATAGATTTTCT
GATATTCCTGAGCTCAAAGATTTCAATCCAGTCGAATTATGTAATTTGGA
ATACAATCCTACTAGAGGGGCATCGATTGATCCTCATTTCGATGATTTCT
GGTTATGGGGCGAGCGGCTGGTAACCATAAATGTGCAATCATCGACTTAT
CTAACTTTCACTCCTGGGTTTCCTGACATGTTCGACGAGAGTTCCCAAGC
TTTCTTTTCTTCAGTATGTTCAGAAAACCACGAAAATATGGGGAATAACT
GCGCGGTATCGATCAAGGTACCTTTGCCCGAGGGTTCCCTGGTGATCGTT
TCCGGTGATGCCAGGCATAAATGGATGCATGCGGTCAGTGCTGCCGACGT
TCAATCAACGAGAATCGCCTCAACATTGCGGGAATTAAGCATGGAATTCA
CTGAGAACGACCGGGAACTAAGCAGGAAATTGATTGATCTTTCTCTTACC
TTCAATGGGCAGCCTGTGAATTGATCGATAAATTATGTGATTTGTTTCTC
GGTTTTATTTGGCATCTATTGTTTTATTGAATAAATTGGGCTCTTTTATT
TAACCCCCCCCCACCCTGTGCCCGATTTCACATTATTTCCCATTCCATAA
TTCAGCAGCACAATTCCAAAGAATCCCCTCTTTGCATGATTCGGTAAACT
GGCAAAAAATTTTATGCGTTTTTAGTAACCTTTTATAATAATACAATTTT
TGTTCTCAACCTCAAGATCATATCATTCTGATACTAATTTTGAAAAATTT
GAGCCGTATTTTTTGACAAAAAATATTTTCAATGAAATTTTTTATCGTAA
GTATTTGTCTCAAAAAACTTATTTTTTTAAAATGATTATATTTTGATTTA
TTAAAGAAGTGTACCATCTTTATTTCTGAGTCAATTACACTTTTCAACTC
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protein sequence of SMED30031264-orf-1

>SMED30031264-orf-1 ID=SMED30031264-orf-1|Name=SMED30031264-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=288bp
MKLCTCKGIRTCSSCNPNKIKIENQNCIVCYFCPKISKIVKENCISDLKC
EEFHKVNIELNGIILIENFLTEDDKNYLLGGICSNSWVDSQSGRRKQDFG
PKVNFKKRKINLTKFQGLPEYIERFVNRFSDIPELKDFNPVELCNLEYNP
TRGASIDPHFDDFWLWGERLVTINVQSSTYLTFTPGFPDMFDESSQAFFS
SVCSENHENMGNNCAVSIKVPLPEGSLVIVSGDARHKWMHAVSAADVQST
RIASTLRELSMEFTENDRELSRKLIDLSLTFNGQPVN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0055113epiboly involved in gastrulation with mouth forming second
GO:0055114oxidation-reduction process
GO:0006351transcription, DNA-templated
GO:0006355regulation of transcription, DNA-templated
GO:0006482protein demethylation
GO:0031032actomyosin structure organization
GO:0036090cleavage furrow ingression
GO:0070988demethylation
Vocabulary: INTERPRO
TermDefinition
IPR037151AlkB-like_sf
IPR032857ALKBH4
IPR027450AlkB-like
IPR005123Oxoglu/Fe-dep_dioxygenase
Vocabulary: molecular function
TermDefinition
GO:0016491oxidoreductase activity
GO:0003779actin binding
GO:0046872metal ion binding
GO:0051213dioxygenase activity
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005737cytoplasm
GO:0030496midbody
GO:0070938contractile ring
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR037151Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamilyGENE3DG3DSA:2.60.120.590coord: 41..262
e-value: 9.5E-30
score: 105.7
IPR032857Alpha-ketoglutarate-dependent dioxygenase alkB homologue 4PANTHERPTHR12463OXYGENASE-RELATEDcoord: 3..285
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 137..260
score: 9.659
NoneNo IPR availableSUPERFAMILYSSF51197Clavaminate synthase-likecoord: 52..259