Rep

Overview
NameRep
Smed IDSMED30031254
Length (bp)1586
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Rep (SMED30031254) t-SNE clustered cells

Violin plots show distribution of expression levels for Rep (SMED30031254) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Rep (SMED30031254) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Rep (SMED30031254) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30031254

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30031254h1SMcG0018195 SmedASXL_015032SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Rep vs. TrEMBL
Match: Q77I17 (Rep OS=Planaria asexual strain-specific virus-like element type 1 OX=159252 GN=rep PE=4 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 4.724e-16
Identity = 29/63 (46.03%), Postives = 42/63 (66.67%), Query Frame = 3 
Query: 1014 PLINLLDWKSHIFVGESGIGKTNFALSHFKRPVHVKHLQMLNRVTSDISSCDGVVFDDLYFNK 1202
            P  + +  +S +FVG+SGIGKTNFALSHF++P+HV   Q L ++ S     DG V DD++  +
Sbjct:  139 PETDEVSMRSWVFVGKSGIGKTNFALSHFEQPLHVTEKQELRKLGSTKFPYDGFVLDDVHLAE 201

HSP 2 Score: 38.891 bits (89), Expect = 4.724e-16
Identity = 28/136 (20.59%), Postives = 60/136 (44.12%), Query Frame = 2 
Query:  629 NGFRHLQVYLEIYSNMSVYAIRSKLDVPFIRGIAGADRPHAIEYATKADTRISGLVGFRIEEPSKFSAKKIIDKDHFSXXXXXXXXXXXIEILEKHEAFHFMTKKNN---------HGITHAKIRSKYISKLYQFL 1009
            +GFRH+Q+++      +   +  K  V F+  +   D    ++Y TK DTR+   V  +  E ++ S     D++ ++  +N  +    +E++E+     ++  +             +T AK +S +    Y F+
Sbjct:    4 SGFRHIQLFITCKYATTASNVAKKFGVGFVGVVESHDVQKWVDYCTKVDTRVENGVTIKHGERARLSQ----DQEFWAKFLNVNSEREGMEMVERERPLDYIRFREKLEGALLHKLGSVTQAKFKSGWRWPYYGFM 135

HSP 3 Score: 34.2686 bits (77), Expect = 4.724e-16
Identity = 17/52 (32.69%), Postives = 26/52 (50.00%), Query Frame = 1 
Query: 1225 LVDCAFENAVNIKYSVAMIPRGIPRIFCINDIQMFYPEGVSDSCMDAIKRRI 1380
            ++DC F   + + Y  A IP G+ RI C+N +            ++AI RRI
Sbjct:  210 VLDCRFACTMRVLYGTAHIPAGLRRIICVNSLDFVINPTWPPEKIEAIYRRI 261
[Close]
BLAST of Rep vs. TrEMBL
Match: Q910M2 (Rep OS=Planaria asexual strain-specific virus-like element type 2 OX=159253 GN=rep PE=4 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 4.724e-16
Identity = 29/63 (46.03%), Postives = 42/63 (66.67%), Query Frame = 3 
Query: 1014 PLINLLDWKSHIFVGESGIGKTNFALSHFKRPVHVKHLQMLNRVTSDISSCDGVVFDDLYFNK 1202
            P  + +  +S +FVG+SGIGKTNFALSHF++P+HV   Q L ++ S     DG V DD++  +
Sbjct:  139 PETDEVSMRSWVFVGKSGIGKTNFALSHFEQPLHVTEKQELRKLGSTKFPYDGFVLDDVHLAE 201

HSP 2 Score: 38.891 bits (89), Expect = 4.724e-16
Identity = 28/136 (20.59%), Postives = 60/136 (44.12%), Query Frame = 2 
Query:  629 NGFRHLQVYLEIYSNMSVYAIRSKLDVPFIRGIAGADRPHAIEYATKADTRISGLVGFRIEEPSKFSAKKIIDKDHFSXXXXXXXXXXXIEILEKHEAFHFMTKKNN---------HGITHAKIRSKYISKLYQFL 1009
            +GFRH+Q+++      +   +  K  V F+  +   D    ++Y TK DTR+   V  +  E ++ S     D++ ++  +N  +    +E++E+     ++  +             +T AK +S +    Y F+
Sbjct:    4 SGFRHIQLFITCKYATTASNVAKKFGVGFVGVVESHDVQKWVDYCTKVDTRVENGVTIKHGERARLSQ----DQEFWAKFLNVNSEREGMEMVERERPLDYIRFREKLEGALLHKLGSVTQAKFKSGWRWPYYGFM 135

HSP 3 Score: 34.2686 bits (77), Expect = 4.724e-16
Identity = 17/52 (32.69%), Postives = 26/52 (50.00%), Query Frame = 1 
Query: 1225 LVDCAFENAVNIKYSVAMIPRGIPRIFCINDIQMFYPEGVSDSCMDAIKRRI 1380
            ++DC F   + + Y  A IP G+ RI C+N +            ++AI RRI
Sbjct:  210 VLDCRFACTMRVLYGTAHIPAGLRRIICVNSLDFVINPTWPPEKIEAIYRRI 261
[Close]
BLAST of Rep vs. TrEMBL
Match: A0A2K9LW85 (Rep OS=uncultured virus OX=340016 GN=Rep PE=4 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.124e-12
Identity = 36/75 (48.00%), Postives = 47/75 (62.67%), Query Frame = 3 
Query: 1002 NFCVPLINLLDWKSHIFVGESGIGKTNFALSHFKRPVHVKHLQMLNRVTSDISSCDGVVFDDLYFNKLRLEEFLS 1226
            +F  PL  LLD K+ +  G +GIGKT +AL+HF RP+ V HL  L R++ D    DGVVFDDL F+ L     L+
Sbjct:  167 DFSRPL-ELLD-KAVVLYGATGIGKTEYALAHFARPLLVTHLDALARLSPD---HDGVVFDDLDFSHLHFSAVLN 236

HSP 2 Score: 43.1282 bits (100), Expect = 1.124e-12
Identity = 21/53 (39.62%), Postives = 32/53 (60.38%), Query Frame = 1 
Query: 1225 LVDCAFENAVNIKYSVAMIPRGIPRIFCINDIQMFYPEGVSDSCMDAIKRRIK 1383
            L+D A +  V+I+Y+ A +PRG+ RIF  N   +F    + DS   AI RR++
Sbjct:  237 LLDLAQDRDVHIRYTTARLPRGLRRIFTHNTGAIFNIPNLPDSQQAAIDRRVR 289
[Close]
BLAST of Rep vs. TrEMBL
Match: A0A5Q2F4S9 (Replication associated protein OS=Antarctic virus COCH21_51 OX=2664243 GN=rep PE=4 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 4.866e-12
Identity = 26/57 (45.61%), Postives = 40/57 (70.18%), Query Frame = 3 
Query: 1029 LDWKSHIFVGESGIGKTNFALSHFKRPVHVKHLQMLNRVTSDISSCDGVVFDDLYFN 1199
            L+ ++ IF+G SG+GKT FA+SHFK P+ V H+  L ++  +I   DG++FDD+ F 
Sbjct:  178 LNTRAVIFMGRSGLGKTQFAISHFKEPLIVSHIDDLKKLNPNI---DGIIFDDMNFQ 231

HSP 2 Score: 45.0542 bits (105), Expect = 4.866e-12
Identity = 23/63 (36.51%), Postives = 33/63 (52.38%), Query Frame = 1 
Query: 1225 LVDCAFENAVNIKYSVAMIPRGIPRIFCIN-DIQMFYPEGVSDSCMDAIKRRIKIHYFHNKLY 1410
            L D     ++N+KY    IP   PRIF  N D +  + +  +   M+AI+RR  I  F N+LY
Sbjct:  241 LCDMELPRSINVKYGTVEIPANTPRIFTSNRDFEEIWSKDCTFEEMNAIRRRCDITTFENELY 303
[Close]
BLAST of Rep vs. TrEMBL
Match: A0A075LYR7 (Replication-associated protein OS=McMurdo Ice Shelf pond-associated circular DNA virus-2 OX=1521386 PE=4 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 2.209e-11
Identity = 26/57 (45.61%), Postives = 39/57 (68.42%), Query Frame = 3 
Query: 1029 LDWKSHIFVGESGIGKTNFALSHFKRPVHVKHLQMLNRVTSDISSCDGVVFDDLYFN 1199
            L+ ++ IF+G SG+GKT FA+SHFK P+ V H+  L ++   I   DG++FDD+ F 
Sbjct:  178 LNTRAVIFMGRSGLGKTQFAISHFKDPLIVSHIDDLKKLNPTI---DGIIFDDMNFQ 231

HSP 2 Score: 44.669 bits (104), Expect = 2.209e-11
Identity = 23/63 (36.51%), Postives = 33/63 (52.38%), Query Frame = 1 
Query: 1225 LVDCAFENAVNIKYSVAMIPRGIPRIFCIN-DIQMFYPEGVSDSCMDAIKRRIKIHYFHNKLY 1410
            L D     ++N+KY    IP   PRIF  N D +  + +  +   M+AI+RR  I  F N+LY
Sbjct:  241 LCDMELPRSINVKYGTVEIPANTPRIFTSNRDFEEIWSKDCTFEEMNAIRRRCDITTFDNELY 303
[Close]
BLAST of Rep vs. Planmine SMEST
Match: SMESG000039190.1 (SMESG000039190.1)

HSP 1 Score: 225.328 bits (573), Expect = 3.404e-72
Identity = 107/107 (100.00%), Postives = 107/107 (100.00%), Query Frame = 2 
Query:  107 MDDTHNLLKSVQYIGISGGSCSGKTTISKLLQQELQKFENIYVYVLNVDEFYWVRGDCNHQKDEKLNCEIYDIPSSINWNKLMRKIIQISQNYNLIIVEGIMIFQNP 427
            MDDTHNLLKSVQYIGISGGSCSGKTTISKLLQQELQKFENIYVYVLNVDEFYWVRGDCNHQKDEKLNCEIYDIPSSINWNKLMRKIIQISQNYNLIIVEGIMIFQNP
Sbjct:    1 MDDTHNLLKSVQYIGISGGSCSGKTTISKLLQQELQKFENIYVYVLNVDEFYWVRGDCNHQKDEKLNCEIYDIPSSINWNKLMRKIIQISQNYNLIIVEGIMIFQNP 107
[Close]
BLAST of Rep vs. Planmine SMEST
Match: SMESG000055972.1 (SMESG000055972.1)

HSP 1 Score: 120.939 bits (302), Expect = 8.602e-49
Identity = 55/88 (62.50%), Postives = 71/88 (80.68%), Query Frame = 3 
Query:  942 RTIMESLTQKFGQNTSPNFT-NFCVPLINLLDWKSHIFVGESGIGKTNFALSHFKRPVHVKHLQMLNRVTSDISSCDGVVFDDLYFNK 1202
            +TI+E+L   FG +T+ N + N  +P +NLLDWKSH+F+G+S IGKT FALSHF +PVHVKHLQMLN +TS++  CDG+VF DLYFNK
Sbjct:   90 KTILETLINIFGNSTTHNLSENVLIPKMNLLDWKSHVFLGDSVIGKTYFALSHFNKPVHVKHLQMLNIITSNVDECDGIVFHDLYFNK 177

HSP 2 Score: 63.929 bits (154), Expect = 8.602e-49
Identity = 38/89 (42.70%), Postives = 57/89 (64.04%), Query Frame = 2 
Query:  674 MSVYAIRSKLDVPFIRGIAGADRPHAIEYATKADTRISGLVGFRIEEPSKFSAKKIIDKDHFSXXXXXXXXXXXIEILEKHEAFHFMTK 940
            MSV+  R++  +PF+RGIAG DR  A  Y+TK D R+ G +  +I + +K+S+  +   D+F MA+ A  M  A+ IL   E+FH+MTK
Sbjct:    1 MSVFIFRARSGIPFVRGIAGPDRQWASLYSTKDDLRVLGGLSIKIVQLNKYSS-LVQATDNFKMALEAKTMKEALNILMTKESFHYMTK 88

HSP 3 Score: 49.6766 bits (117), Expect = 8.602e-49
Identity = 21/25 (84.00%), Postives = 23/25 (92.00%), Query Frame = 1 
Query: 1237 AFENAVNIKYSVAMIPRGIPRIFCI 1311
            A+EN VNIKYSVAMIP+GIP IFCI
Sbjct:  178 AYENVVNIKYSVAMIPKGIPHIFCI 202
[Close]
BLAST of Rep vs. Planmine SMEST
Match: SMESG000059230.1 (SMESG000059230.1)

HSP 1 Score: 78.9518 bits (193), Expect = 4.302e-15
Identity = 38/92 (41.30%), Postives = 58/92 (63.04%), Query Frame = 1 
Query: 1138 IGLRQTSLVVTGLSLMIYILIS*D*KNFCLVDCAFENAVNIKYSVAMIPRGIPRIFCINDIQMFYPEGVSDSCMDAIKRRIKIHYFHN-KLY 1410
            IG     LV+  ++   ++  +       +VDC F+ +VN+KY V+ IP G+PRI C+NDIQ+F+P+    + M+AIKRRI +H F + KLY
Sbjct:  658 IGASTDGLVIDDMTFRSWLPTNL----LHIVDCEFDRSVNVKYGVSTIPAGLPRIICVNDIQLFWPQDCLPATMEAIKRRIVLHDFRDMKLY 745

HSP 2 Score: 72.7886 bits (177), Expect = 3.304e-13
Identity = 36/87 (41.38%), Postives = 56/87 (64.37%), Query Frame = 3 
Query:  936 QKRTIMESLTQKFGQNTSPNFTNFCVPLINLLDWKSHIFVGESGIGKTNFALSHFKRPVHVKHLQMLNRVTSDISSCDGVVFDDLYF 1196
            Q++ I   L ++  +N +  FT+F  P I++ D K+HIF+G +GIGKTNFAL+HFK+P+     Q   ++    +S DG+V DD+ F
Sbjct:  589 QRQKIEACLIREARRNVNAKFTSFNRPFIDVNDKKTHIFIGGTGIGKTNFALAHFKKPILCSDRQDFGKIG---ASTDGLVIDDMTF 672
[Close]
BLAST of Rep vs. Planmine SMEST
Match: SMESG000069836.1 (SMESG000069836.1)

HSP 1 Score: 56.6102 bits (135), Expect = 2.264e-10
Identity = 31/82 (37.80%), Postives = 49/82 (59.76%), Query Frame = 3 
Query:  978 QNTSPNFTNFCVPLINLLDWKSHIFVGESGIGKTNFALSHFKRPVHVKHLQMLNRVTSDISSCDGVVFDDLYFNKLRLEEFL 1223
            QN+  N   + +P  +L D ++ + +G +G+GKTN+ LSHFK P+HV   Q  +++  D    DGVV DD+  +K   EE +
Sbjct:   55 QNSKFN-EGYRIPYQDLKD-RTLVLIGGTGVGKTNYTLSHFKDPLHVTIRQDFDKMGPDNHVYDGVVIDDVALSKWDPEEVI 134

HSP 2 Score: 27.7202 bits (60), Expect = 2.264e-10
Identity = 14/49 (28.57%), Postives = 25/49 (51.02%), Query Frame = 1 
Query: 1225 LVDCAFENAVNIKYSVAMIPRGIPRIFCINDIQ-MFYPEGVSDSCMDAI 1368
            L D  + +++N+KY + +I     RI C N I  +  P   +   +DA+
Sbjct:  136 LCDRNYSSSINVKYGIGIIDASTQRIICANSITDIINPAWNTKRYIDAV 184
[Close]
BLAST of Rep vs. Planmine SMEST
Match: SMESG000069836.1 (SMESG000069836.1)

HSP 1 Score: 55.4546 bits (132), Expect = 4.862e-10
Identity = 25/62 (40.32%), Postives = 39/62 (62.90%), Query Frame = 3 
Query: 1038 KSHIFVGESGIGKTNFALSHFKRPVHVKHLQMLNRVTSDISSCDGVVFDDLYFNKLRLEEFL 1223
            ++ + +G +G+GKTN+ LSHFK P+HV   Q  +++  D    DGVV DD+  +K   EE +
Sbjct:   52 RTLVLIGGTGVGKTNYTLSHFKDPLHVTIRQDFDKMGPDNHVYDGVVIDDVALSKWDPEEVI 113

HSP 2 Score: 27.7202 bits (60), Expect = 4.862e-10
Identity = 14/49 (28.57%), Postives = 25/49 (51.02%), Query Frame = 1 
Query: 1225 LVDCAFENAVNIKYSVAMIPRGIPRIFCINDIQ-MFYPEGVSDSCMDAI 1368
            L D  + +++N+KY + +I     RI C N I  +  P   +   +DA+
Sbjct:  115 LCDRNYSSSINVKYGIGIIDASTQRIICANSITDIINPAWNTKRYIDAV 163
The following BLAST results are available for this feature:
BLAST of Rep vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
Q77I174.724e-1646.03Rep OS=Planaria asexual strain-specific virus-like... [more]
Q910M24.724e-1646.03Rep OS=Planaria asexual strain-specific virus-like... [more]
A0A2K9LW851.124e-1248.00Rep OS=uncultured virus OX=340016 GN=Rep PE=4 SV=1[more]
A0A5Q2F4S94.866e-1245.61Replication associated protein OS=Antarctic virus ... [more]
A0A075LYR72.209e-1145.61Replication-associated protein OS=McMurdo Ice Shel... [more]
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BLAST of Rep vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000039190.13.404e-72100.00SMESG000039190.1[more]
SMESG000055972.18.602e-4962.50SMESG000055972.1[more]
SMESG000059230.14.302e-1541.30SMESG000059230.1[more]
SMESG000069836.12.264e-1037.80SMESG000069836.1[more]
SMESG000069836.14.862e-1040.32SMESG000069836.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031254 ID=SMED30031254|Name=Rep|organism=Schmidtea mediterranea sexual|type=transcript|length=1586bp
AAATAATTACCGATATCACATTCTTCATGAAATTCAGGAAATATATTTTC
AGAAAATAGTTTTATGAATTTTTAAATTATCAATTTAATCAAATAACAAA
GTTTTCATGGATGATACCCATAATTTATTAAAATCTGTTCAATATATTGG
AATATCTGGCGGGAGTTGCAGCGGTAAGACGACAATTAGTAAACTACTGC
AACAAGAATTACAAAAATTCGAAAATATATATGTTTATGTTTTGAATGTG
GATGAATTTTACTGGGTCCGAGGTGACTGCAATCATCAGAAGGATGAAAA
ACTTAATTGTGAAATTTATGACATACCGTCATCAATAAATTGGAATAAAC
TAATGAGAAAAATAATACAGATTTCACAGAATTATAATTTAATTATTGTA
GAAGGAATAATGATATTTCAAAATCCTTCTCAAAGCACTTCAAAGAACAA
TATTCACCAACAAGTCGATTCGAATGCTGCAGGATGCGATTATGTCGGAA
GTAGAAGATATAACTCCTTCGCTTTCAACATATCTATGCGAAAGTATCGC
GAGCTGGAGGGCAGGCTTCAAGAGATTTTCAATAACTCCAAATTTACCCG
TTTCGTGGTGGCACAGGAAGAGGGTGAAAATGGATTCCGTCATTTGCAGG
TCTATCTCGAGATTTATAGCAACATGTCAGTTTATGCCATTAGATCTAAG
CTCGATGTCCCATTTATTCGAGGAATCGCTGGGGCTGATAGACCCCATGC
CATCGAATATGCAACCAAGGCAGATACCCGAATCTCTGGATTAGTCGGAT
TCCGAATCGAAGAGCCAAGTAAATTTTCAGCTAAAAAAATTATAGATAAA
GATCACTTTTCAATGGCAATGAATGCTCCGAACATGGCAGCGGCTATCGA
AATTTTAGAAAAACATGAGGCATTCCATTTTATGACAAAAAAGAACAATC
ATGGAATCACTCACGCAAAAATTCGGTCAAAATACATCTCCAAACTTTAC
CAATTTTTGTGTTCCTTTAATTAATCTGCTTGACTGGAAAAGTCATATAT
TTGTTGGTGAAAGTGGAATAGGGAAAACTAACTTTGCTTTGTCTCACTTT
AAACGACCGGTTCATGTTAAACATTTACAAATGCTGAATCGGGTTACGTC
AGACATCTCTAGTTGTGACGGGGTTGTCTTTGATGATTTATATTTTAATA
AGTTGAGACTAGAAGAATTTCTGTCTTGTTGATTGTGCGTTTGAAAACGC
TGTAAATATTAAGTATTCTGTGGCGATGATACCACGAGGAATCCCAAGAA
TATTCTGTATTAATGATATACAAATGTTTTACCCAGAAGGTGTTTCGGAT
AGCTGCATGGATGCCATTAAACGGAGAATTAAAATTCATTATTTTCATAA
TAAGCTATATAAAACATATTTCTGAAAAAGCACTGACTCACCCATACCAG
CAGACGAGCAGCGTGTAACTGAGAATAAACAGAAAACAACGTTTACTAAC
TTGGTGTGTACGAATTGCAACACGAAACTGAGACTGTATCTCAGTGTCTG
CAAAATTAATTTTTATTAATGTTAAATGTTTATGTG
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protein sequence of SMED30031254-orf-1

>SMED30031254-orf-1 ID=SMED30031254-orf-1|Name=SMED30031254-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=306bp
MDDTHNLLKSVQYIGISGGSCSGKTTISKLLQQELQKFENIYVYVLNVDE
FYWVRGDCNHQKDEKLNCEIYDIPSSINWNKLMRKIIQISQNYNLIIVEG
IMIFQNPSQSTSKNNIHQQVDSNAAGCDYVGSRRYNSFAFNISMRKYREL
EGRLQEIFNNSKFTRFVVAQEEGENGFRHLQVYLEIYSNMSVYAIRSKLD
VPFIRGIAGADRPHAIEYATKADTRISGLVGFRIEEPSKFSAKKIIDKDH
FSMAMNAPNMAAAIEILEKHEAFHFMTKKNNHGITHAKIRSKYISKLYQF
LCSFN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0006259DNA metabolic process
GO:0018142protein-DNA covalent cross-linking
GO:0034356NAD biosynthesis via nicotinamide riboside salvage pathway
GO:0006260DNA replication
Vocabulary: molecular function
TermDefinition
GO:0016779nucleotidyltransferase activity
GO:0016888endodeoxyribonuclease activity, producing 5'-phosphomonoesters
GO:0050262ribosylnicotinamide kinase activity
GO:0005198structural molecule activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR026681NRK
IPR027417P-loop_NTPase
Vocabulary: cellular component
TermDefinition
GO:0019028viral capsid
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 6..123
e-value: 1.6E-17
score: 65.6
NoneNo IPR availableGENE3DG3DSA:3.30.70.2090coord: 137..233
e-value: 1.8E-7
score: 33.3
NoneNo IPR availablePANTHERPTHR10285URIDINE KINASEcoord: 14..122
IPR026681Nicotinamide riboside kinasePANTHERPTHR10285:SF158SD05789P2coord: 14..122
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 10..108