G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30031214
Length (bp)1154
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30031214) t-SNE clustered cells

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30031214) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30031214) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30031214) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30031214

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30031214h1SMcG0022528 Contig4610newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30031214h1SMcG0022528 Contig4610uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
head regionSMED30031214h1SMcG0021507 SmedASXL_001619SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
cephalic gangliaSMED30031214h1SMcG0021507 dd_Smed_v4_16815_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30031214h1SMcG0021507 dd_Smed_v4_16815_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A4S2L946 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_010022 PE=4 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 1.635e-19
Identity = 69/185 (37.30%), Postives = 112/185 (60.54%), Query Frame = 2 
Query:  197 KKGIWLSTRMFMTIMGIVDLFALVLFIIFNIEK---PLIELELLNFEMISKWFNVKMILAEFLHGLIFISNWITVLLGFERYIAVCYPLYSRRIDYIHRXXXXXXXXXXXXXXXXXXXXXXYLHRFHDLRIQ-RNFIYKLYTH-VQLWIITLFLFLIVPFVCLALINFQLILAVRRSSRFLRQCS 736
            ++GIW  T  ++ ++  VDL  + L  + +++    PL E +L        + +++ +++E    L+FISNW+T +L  ER+IA+CYPL SRRI   HRK+AV ++  + +I KLP  +      FH+L     NF+Y  + H + +WI+ LFLFLI+PF  L  +N +LI+A+R+S+  LR  S
Sbjct:   49 RRGIWRPTVTYIIVLASVDLCTVTLHCLLSLDTIFLPLFEPDL--------YTSLENVVSEVADTLLFISNWLTTILATERFIAICYPLQSRRIGKRHRKVAVLIVIFLSVIVKLPGFL------FHNLSGSINNFLYVSFLHRLYVWIVQLFLFLIIPFAILTFVNIRLIIAIRKSANLLRTLS 219
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A4S2L995 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_010022 PE=4 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 1.711e-19
Identity = 69/185 (37.30%), Postives = 112/185 (60.54%), Query Frame = 2 
Query:  197 KKGIWLSTRMFMTIMGIVDLFALVLFIIFNIEK---PLIELELLNFEMISKWFNVKMILAEFLHGLIFISNWITVLLGFERYIAVCYPLYSRRIDYIHRXXXXXXXXXXXXXXXXXXXXXXYLHRFHDLRIQ-RNFIYKLYTH-VQLWIITLFLFLIVPFVCLALINFQLILAVRRSSRFLRQCS 736
            ++GIW  T  ++ ++  VDL  + L  + +++    PL E +L        + +++ +++E    L+FISNW+T +L  ER+IA+CYPL SRRI   HRK+AV ++  + +I KLP  +      FH+L     NF+Y  + H + +WI+ LFLFLI+PF  L  +N +LI+A+R+S+  LR  S
Sbjct:   71 RRGIWRPTVTYIIVLASVDLCTVTLHCLLSLDTIFLPLFEPDL--------YTSLENVVSEVADTLLFISNWLTTILATERFIAICYPLQSRRIGKRHRKVAVLIVIFLSVIVKLPGFL------FHNLSGSINNFLYVSFLHRLYVWIVQLFLFLIIPFAILTFVNIRLIIAIRKSANLLRTLS 241
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A419PUG7 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Clonorchis sinensis OX=79923 GN=CSKR_6840s PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 1.923e-19
Identity = 69/185 (37.30%), Postives = 113/185 (61.08%), Query Frame = 2 
Query:  197 KKGIWLSTRMFMTIMGIVDLFALVLFIIFNIEK---PLIELELLNFEMISKWFNVKMILAEFLHGLIFISNWITVLLGFERYIAVCYPLYSRRIDYIHRXXXXXXXXXXXXXXXXXXXXXXYLHRFHDLRIQ-RNFIYKLYTH-VQLWIITLFLFLIVPFVCLALINFQLILAVRRSSRFLRQCS 736
            ++GIW  T  ++ ++  VDL  + L  + +++    PL E +L        + +++ +++E    L+FISNW+T +L  ER+IA+CYPL SRRI   HRK+AV ++ ++ +I KLP  +      FH+L     NF+Y  + H + +WI+ LFLFLI+PF  L  +N +LI+A+R+S+  LR  S
Sbjct:   71 RRGIWRPTVTYIIVLASVDLCTVTLHCLLSLDTIFLPLFEPDL--------YTSLENVVSEVADTLLFISNWLTTILATERFIAICYPLQSRRIGKRHRKVAVLIVILLSVIVKLPGFL------FHNLSGSINNFLYISFLHRLYVWIVQLFLFLIIPFAILTFVNIRLIIAIRKSANLLRTLS 241
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: G7YEN5 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Clonorchis sinensis OX=79923 GN=CLF_106081 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 2.070e-19
Identity = 69/185 (37.30%), Postives = 113/185 (61.08%), Query Frame = 2 
Query:  197 KKGIWLSTRMFMTIMGIVDLFALVLFIIFNIEK---PLIELELLNFEMISKWFNVKMILAEFLHGLIFISNWITVLLGFERYIAVCYPLYSRRIDYIHRXXXXXXXXXXXXXXXXXXXXXXYLHRFHDLRIQ-RNFIYKLYTH-VQLWIITLFLFLIVPFVCLALINFQLILAVRRSSRFLRQCS 736
            ++GIW  T  ++ ++  VDL  + L  + +++    PL E +L        + +++ +++E    L+FISNW+T +L  ER+IA+CYPL SRRI   HRK+AV ++ ++ +I KLP  +      FH+L     NF+Y  + H + +WI+ LFLFLI+PF  L  +N +LI+A+R+S+  LR  S
Sbjct:   71 RRGIWRPTVTYIIVLASVDLCTVTLHCLLSLDTIFLPLFEPDL--------YTSLENVVSEVADTLLFISNWLTTILATERFIAICYPLQSRRIGKRHRKVAVLIVILLSVIVKLPGFL------FHNLSGSINNFLYISFLHRLYVWIVQLFLFLIIPFAILTFVNIRLIIAIRKSANLLRTLS 241
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A183A6C3 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS2508 PE=4 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 6.922e-17
Identity = 68/178 (38.20%), Postives = 105/178 (58.99%), Query Frame = 2 
Query:  197 KKGIWLSTRMFMTIMGIVDLFALVLFIIFNIEKPLIELELLNFEMISKWF-NVKMILAEFLHGLIFISNWITVLLGFERYIAVCYPLYSRRIDYIHRXXXXXXXXXXXXXXXXXXXXXXYLHRFHDLRIQRNFIYKLYTHVQLWIITLFLFLIVPFVCLALINFQLILAVRRSSRFLR 727
            ++G+W  T  ++ ++ IVDL  L+L  I +++  +I      F M   WF + + +L E +  L+FISNW+T +L  ERY+A+CYPL SRRI   HR+I V ++ V  +I + P  ++  L+  +D     N    +Y    +WI+ + LFLI PF  L  +N +LI AVRRSS+ LR
Sbjct:   67 RRGLWRPTVTYVVMLAIVDLLILLLLSILSLDYFIIPWTSEKFRM---WFYSAETVLGESVDTLLFISNWLTAMLATERYVAICYPLQSRRISRKHRRIVVTMVIVSSLIIRTPGFVL--LNMSNDPVTYPNI--AVYQRCYVWIVQVTLFLIAPFALLTFVNLRLIQAVRRSSKLLR 237
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000078268.1 (SMESG000078268.1)

HSP 1 Score: 509.605 bits (1311), Expect = 0.000e+0
Identity = 350/351 (99.72%), Postives = 351/351 (100.00%), Query Frame = 2 
Query:   59 MLNSTSGIDKKNESVLSVYIALKTPLXXXXXXXXXXXXXXXXXXALKKGIWLSTRMFMTIMGIVDLFALVLFIIFNIEKPLIELELLNFEMISKWFNVKMILAEFLHGLIFISNWITVLLGFERYIAVCYPLYSRRIDYIHRXXXXXXXXXXXXXXXXXXXXXXYLHRFHDLRIQRNFIYKLYTHVQLWIITLFLFLIVPFVCLALINFQLILAVRRSSRFLRQCSXXXXXXXXXXSRTEEHKITXXXXXXXXXXXXXXXXXXXYVTLQSFNYHXXXXXXXXXXXXXXXXXVHFFLYCWFCEKFRQILYHLLCRSSRQEISSFTLADQSTQQLMYPISPTLEQRNAKANIC 1111
            MLNSTSGIDKKNESVLSVYIALKTPLTLFISIISIILIFGTIVIALKKGIWLSTRMFMTIMGIVDLFALVLFIIFNIEKPLIELELLNFEMISKWFNVKMILAEFLHGLIFISNWITVLLGFERYIAVCYPLYSRRIDYIHRKIAVAVIFVVIIIFKLPLIIMIYLHRFHDLRIQRNFIYKLYTHVQLWIITLFLFLIVPFVCLALINFQLILAVRRSSRFLRQCSNQNPNYQN+NSRTEEHKITFVLIGIIVIFFVFQGPFGIYVTLQSFNYHSISLRGISSILLSLKSIVHFFLYCWFCEKFRQILYHLLCRSSRQEISSFTLADQSTQQLMYPISPTLEQRNAKANIC
Sbjct:    1 MLNSTSGIDKKNESVLSVYIALKTPLTLFISIISIILIFGTIVIALKKGIWLSTRMFMTIMGIVDLFALVLFIIFNIEKPLIELELLNFEMISKWFNVKMILAEFLHGLIFISNWITVLLGFERYIAVCYPLYSRRIDYIHRKIAVAVIFVVIIIFKLPLIIMIYLHRFHDLRIQRNFIYKLYTHVQLWIITLFLFLIVPFVCLALINFQLILAVRRSSRFLRQCSNQNPNYQNSNSRTEEHKITFVLIGIIVIFFVFQGPFGIYVTLQSFNYHSISLRGISSILLSLKSIVHFFLYCWFCEKFRQILYHLLCRSSRQEISSFTLADQSTQQLMYPISPTLEQRNAKANIC 351
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000039899.1 (SMESG000039899.1)

HSP 1 Score: 94.3597 bits (233), Expect = 2.280e-21
Identity = 97/282 (34.40%), Postives = 158/282 (56.03%), Query Frame = 2 
Query:  197 KKGIWLSTRMFMTIMGIVDLFALVLFIIFNIEKPLIELELLNFEMISKWFNVKMILAEFLHGLIFISNWITVLLGFERYIAVCYPLYSRRIDYIHRXXXXXXXXXXXXXXXXXXXXXXYLHRF---HDLRIQRNFIYKLYTHVQL---WIITLFLFLIVPFVCLALINFQLILAVRRSSRFLRQ-CSXXXXXXXXXXSRTEEHKITXXXXXXXXXXXXXXXXXXXYVTL-------QSFNYHXXXXXXXXXXXXXXXXXVHFFLYCWFCEKFRQILYHLLCR 1000
            K+ +W+STR+++T +G+VD+  LV+  I +++  ++   + N  +  + +    IL E    ++ +SNW+T +LG ERY+ V YP  ++RI   HRK+     FV  ++ K+P +I++  +     +D +     I K   + +L   WI+   LFLIVPF  L  +N +LIL +RRSSRF+RQ C+N++          EE KIT ++I +I+ FF+ QGPF  Y  L       Q +      L+ +S ++LS+KS   F L+CW C+KF   L  + C 
Sbjct:   54 KRNLWISTRIYITALGLVDILGLVILTIASLDYFVLNYTI-NMPIFYRIYQYIFILFE---AVLLLSNWLTTILGIERYLVVSYPFLTKRITNSHRKLITLFCFVGTVLVKIPSLILLINYESIVNNDFQQHTQEIEKSLHNFELWQSWIVDFSLFLIVPFCVLIFVNARLILTIRRSSRFMRQYCANRHRKI-----ENEERKITCIMICVIMAFFICQGPFITYTALSNVHLFHQDYKNGQSMLQELSILILSMKSDFSFVLHCWVCDKFGAALKRMFCN 326
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000058691.1 (SMESG000058691.1)

HSP 1 Score: 47.7506 bits (112), Expect = 9.291e-6
Identity = 31/84 (36.90%), Postives = 47/84 (55.95%), Query Frame = 2 
Query:  215 STRMFMTIMGIVDLFALVLFIIFNIEKPLIELELLNFEMISKWFNVKMILAEFLHGLIFIS----NWITVLLGFERYIAVCYPL 454
            ST+  +  + I DL  L + +IF  E  +I +   + E+  K    K I A+FL  L+ IS    NW+TV++G ER++  CYPL
Sbjct:   53 STKFILQTLSITDLLFL-MSMIFYFELKIIYILYPHLELTLKPIKEKFIFADFLVMLVNISELFRNWLTVIIGIERFLVTCYPL 135
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4S2L9461.635e-1937.30G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A4S2L9951.711e-1937.30G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A419PUG71.923e-1937.30G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
G7YEN52.070e-1937.30G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A183A6C36.922e-1738.20G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000078268.10.000e+099.72SMESG000078268.1[more]
SMESG000039899.12.280e-2134.40SMESG000039899.1[more]
SMESG000058691.19.291e-636.90SMESG000058691.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031214 ID=SMED30031214|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1154bp
CTAATTCTTATTGTTTGTTGGTGGTTTTTTTTATGTCAAAGAAGAAACTT
TGAAACTTATGCTAAACTCTACTTCTGGGATTGATAAGAAAAATGAATCC
GTTCTTAGTGTTTATATTGCTTTAAAAACACCTTTGACTTTGTTTATATC
AATCATCAGTATAATATTAATCTTTGGCACAATTGTAATTGCACTGAAAA
AGGGAATATGGCTATCGACAAGAATGTTTATGACAATAATGGGAATTGTG
GATTTGTTTGCGTTGGTATTGTTCATAATTTTCAATATCGAAAAACCTTT
GATCGAATTGGAACTGTTGAATTTCGAAATGATTTCGAAATGGTTCAACG
TCAAAATGATTCTGGCTGAATTTCTCCACGGTTTGATATTTATTTCTAAT
TGGATTACGGTTTTGTTGGGTTTCGAACGCTACATTGCGGTATGTTATCC
GCTTTATTCAAGGCGAATAGATTACATTCACAGAAAAATTGCCGTCGCTG
TTATTTTTGTCGTTATAATCATTTTTAAATTGCCATTAATTATCATGATT
TATCTTCACAGATTTCATGACTTGCGCATTCAAAGGAATTTCATTTACAA
ATTATACACCCATGTTCAATTATGGATAATCACATTGTTTTTGTTTTTAA
TTGTTCCATTTGTTTGCTTGGCATTGATAAATTTTCAGTTGATTCTAGCT
GTGAGAAGATCGTCGAGATTTCTTCGACAGTGTTCCAATCAGAATCCAAA
TTACCAAAACAACAACTCTCGAACAGAAGAACATAAAATAACTTTTGTTT
TAATAGGTATAATTGTAATATTTTTTGTTTTTCAGGGCCCATTTGGAATT
TATGTTACATTACAATCATTTAATTATCATTCTATTTCTTTGAGAGGCAT
AAGTTCAATTTTATTGTCTTTGAAAAGTATTGTGCATTTCTTTTTATACT
GTTGGTTTTGTGAGAAATTTCGACAGATTCTTTATCACTTATTGTGTAGA
AGCTCTCGTCAGGAAATATCATCATTTACACTAGCAGATCAGTCAACTCA
ACAGTTGATGTATCCCATCTCCCCGACACTGGAACAAAGAAATGCCAAAG
CTAATATATGTTAATTTCGTTTGGTTATAATTTGTATTGTGGTTTAGAGA
TCGG
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protein sequence of SMED30031214-orf-1

>SMED30031214-orf-1 ID=SMED30031214-orf-1|Name=SMED30031214-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=352bp
MLNSTSGIDKKNESVLSVYIALKTPLTLFISIISIILIFGTIVIALKKGI
WLSTRMFMTIMGIVDLFALVLFIIFNIEKPLIELELLNFEMISKWFNVKM
ILAEFLHGLIFISNWITVLLGFERYIAVCYPLYSRRIDYIHRKIAVAVIF
VVIIIFKLPLIIMIYLHRFHDLRIQRNFIYKLYTHVQLWIITLFLFLIVP
FVCLALINFQLILAVRRSSRFLRQCSNQNPNYQNNNSRTEEHKITFVLIG
IIVIFFVFQGPFGIYVTLQSFNYHSISLRGISSILLSLKSIVHFFLYCWF
CEKFRQILYHLLCRSSRQEISSFTLADQSTQQLMYPISPTLEQRNAKANI
C*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000276G protein-coupled receptor, rhodopsin-likePRINTSPR00237GPCRRHODOPSNcoord: 106..128
score: 22.0
coord: 141..162
score: 17.46
coord: 54..75
score: 17.32
coord: 244..268
score: 22.95
coord: 279..305
score: 20.75
coord: 189..212
score: 13.74
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 42..286
e-value: 5.2E-11
score: 42.4
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 8..322
e-value: 8.6E-33
score: 115.6
NoneNo IPR availablePANTHERPTHR46641FAMILY NOT NAMEDcoord: 3..318
NoneNo IPR availableCDDcd149787tmA_FMRFamide_R-likecoord: 53..308
e-value: 4.93775E-21
score: 89.6144
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 20..320
NoneNo IPR availableTMHMMTMhelixcoord: 246..268
NoneNo IPR availableTMHMMTMhelixcoord: 53..75
NoneNo IPR availableTMHMMTMhelixcoord: 186..208
NoneNo IPR availableTMHMMTMhelixcoord: 24..46
NoneNo IPR availableTMHMMTMhelixcoord: 278..300
NoneNo IPR availableTMHMMTMhelixcoord: 144..166
NoneNo IPR availableTMHMMTMhelixcoord: 101..123
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 30..297
score: 20.412