SMED30031190

Overview
NameSMED30031190
Smed IDSMED30031190
Length (bp)1891
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30031190 (SMED30031190) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30031190 (SMED30031190) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30031190

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30031190h1SMcG0015227 SmedASXL_000796SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30031190h1SMcG0015227 dd_Smed_v4_18390_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30031190h1SMcG0015227 dd_Smed_v4_18390_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
oocyteSMED30031190h1SMcG0015227 Contig36320newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
oocyteSMED30031190h1SMcG0015227 Contig36320uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30031190h1SMcG0015227 Contig36320newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30031190h1SMcG0015227 Contig36320uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30031190h1SMcG0015227 Contig36320newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30031190h1SMcG0015227 Contig36320uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30031190 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000490:39402..41292 +9455
Homology
[Close]
BLAST of SMED30031190 vs. TrEMBL
Match: A0A504Z4S6 (Uncharacterized protein OS=Fasciola gigantica OX=46835 GN=FGIG_07281 PE=4 SV=1)

HSP 1 Score: 394.815 bits (1013), Expect = 1.082e-123
Identity = 190/339 (56.05%), Postives = 257/339 (75.81%), Query Frame = 1 
Query:   43 DQLPVYPPNIQGELRLVLQNKDLFLLCYKYLEDYRRLNEEQNRIITKDFIESQIKIGDEEIDELQTELTNAEYKLTVRTRHNETLLGQLSVRGMSTLGDGSPRRNI--CRSPQIRKC----PHMSSSIDMKSKDLT---LFENIALAIEEIQREIKNKMKPLCDFYRDICYLQLHFVLVLTQICNYCMPLVTENVGLIDRVFFWSDRPEFELVRKSGEIADRLKDIFTRTQSVLTLLVENVMFLQNKVELQKNLERKFQDRLEVQQLEKDKVLRKIHGSRLDMDLLEEENDRLVGILVKLDPKYVESDHFNELADYSVRRHYLEGRLTPNGFKAHQTNV 1032
            DQLP YP NIQGELR++LQ KD+FL+CYK+LE+YRRL  EQNR+ITK+F++ Q++IG++EI EL+ EL +AE +L VR RHNE L   L+  G ++L  G   R++  CRSPQ+RK        S+++   S+      + +NIALAI EI +E+++K+KPL D+YRD+ YLQLHF LVL Q+CNYCMPLVTEN+GLIDR+FFWS+RPEF+LVR++G+I DRLK+I+TR Q+VL++L E+V FLQ+KVELQK LE++F +RL+ QQ  KDK+LR++H ++ +MD+LEEENDRLVG LVK+DP +V+S HF ELA+       L G   PN  +  Q N 
Sbjct:    9 DQLPTYPNNIQGELRIILQTKDVFLVCYKHLEEYRRLVNEQNRLITKEFVQEQLQIGEDEITELKMELQDAESRLGVRLRHNELLADHLASLGCASLESGGSVRHLALCRSPQMRKNMLAQRQGSAALSTASESRPPTHMIDNIALAIAEIHQEVRSKVKPLLDYYRDLFYLQLHFSLVLGQVCNYCMPLVTENIGLIDRIFFWSERPEFDLVRRAGDIGDRLKEIYTRAQTVLSVLAESVRFLQSKVELQKTLEQRFHERLDEQQKTKDKILREVHTTKQNMDMLEEENDRLVGSLVKVDPSFVDSRHFYELAEGGT----LMGAKKPNMKETAQNNA 343
[Close]
BLAST of SMED30031190 vs. TrEMBL
Match: A0A183ACA6 (Uncharacterized protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS4591 PE=4 SV=1)

HSP 1 Score: 393.66 bits (1010), Expect = 1.104e-123
Identity = 184/315 (58.41%), Postives = 247/315 (78.41%), Query Frame = 1 
Query:   43 DQLPVYPPNIQGELRLVLQNKDLFLLCYKYLEDYRRLNEEQNRIITKDFIESQIKIGDEEIDELQTELTNAEYKLTVRTRHNETLLGQLSVRGMSTLGDGSPRRNI--CRSPQIRK-------CPHMSSSIDMKSKDLTLFENIALAIEEIQREIKNKMKPLCDFYRDICYLQLHFVLVLTQICNYCMPLVTENVGLIDRVFFWSDRPEFELVRKSGEIADRLKDIFTRTQSVLTLLVENVMFLQNKVELQKNLERKFQDRLEVQQLEKDKVLRKIHGSRLDMDLLEEENDRLVGILVKLDPKYVESDHFNELAD 960
            DQLP YP NIQGELR++LQ KD+FL+CYK+LE+YRRL  EQNR+ITK+F++ Q++IG++EI EL+ EL +AE +L  R RHNE L   L+  G ++L  G+  R++  CRSPQ+RK           SSS+        + +NIALAI EI +E++ K+KPL D+YRD+ YLQLHF LVL Q+CNYCMPLVTEN+GLIDR+FFWS+RPEF+LVR++G+I DRLK+I+TR Q+VL++L E+V FLQ+KVELQK LE++F +RL+ QQ  KDK+LR++H ++ +MD+LEEENDRLVG LVK+DP +V+S HF ELA+
Sbjct:    9 DQLPTYPNNIQGELRIILQTKDVFLVCYKHLEEYRRLVNEQNRLITKEFVQEQLQIGEDEISELKMELQDAESRLGARLRHNELLADHLASLGFASLESGASVRHLALCRSPQMRKNMIAQRQGGAGSSSMSEPRPPTHMIDNIALAISEIHQEVRAKVKPLLDYYRDLFYLQLHFSLVLGQVCNYCMPLVTENIGLIDRIFFWSERPEFDLVRRAGDIGDRLKEIYTRAQTVLSVLAESVRFLQSKVELQKTLEQRFHERLDEQQKTKDKILREVHTTKQNMDMLEEENDRLVGSLVKVDPSFVDSRHFYELAE 323
[Close]
BLAST of SMED30031190 vs. TrEMBL
Match: A0A2H1CTU2 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_001096 PE=4 SV=1)

HSP 1 Score: 394.045 bits (1011), Expect = 1.944e-123
Identity = 190/339 (56.05%), Postives = 256/339 (75.52%), Query Frame = 1 
Query:   43 DQLPVYPPNIQGELRLVLQNKDLFLLCYKYLEDYRRLNEEQNRIITKDFIESQIKIGDEEIDELQTELTNAEYKLTVRTRHNETLLGQLSVRGMSTLGDGSPRRNI--CRSPQIRKC----PHMSSSIDMKSKDLT---LFENIALAIEEIQREIKNKMKPLCDFYRDICYLQLHFVLVLTQICNYCMPLVTENVGLIDRVFFWSDRPEFELVRKSGEIADRLKDIFTRTQSVLTLLVENVMFLQNKVELQKNLERKFQDRLEVQQLEKDKVLRKIHGSRLDMDLLEEENDRLVGILVKLDPKYVESDHFNELADYSVRRHYLEGRLTPNGFKAHQTNV 1032
            DQLP YP NIQGELR++LQ KD+FL+CYK+LE+YRRL  EQNR+ITK+F++ Q++IG++EI EL+ EL +AE +L VR RHNE L   L+  G ++L  G   R++  CRSPQ+RK        S ++   S+      + +NIALAI EI +E+++K+KPL D+YRD+ YLQLHF LVL Q+CNYCMPLVTEN+GLIDR+FFWS+RPEF+LVR++G+I DRLK+I+TR Q+VL++L E+V FLQ+KVELQK LE++F +RL+ QQ  KDK+LR++H ++ +MD+LEEENDRLVG LVK+DP +V+S HF ELA+       L G   PN  +  Q N 
Sbjct:    9 DQLPTYPNNIQGELRIILQTKDVFLVCYKHLEEYRRLVNEQNRLITKEFVQEQLQIGEDEITELKMELQDAESRLGVRLRHNELLADHLASLGCASLESGGSVRHLALCRSPQMRKNMLAQRQGSGALSTASESRPPTHMIDNIALAIAEIHQEVRSKVKPLLDYYRDLFYLQLHFSLVLGQVCNYCMPLVTENIGLIDRIFFWSERPEFDLVRRAGDIGDRLKEIYTRAQTVLSVLAESVRFLQSKVELQKTLEQRFHERLDEQQKTKDKILREVHTTKQNMDMLEEENDRLVGSLVKVDPSFVDSRHFYELAEGGT----LMGAKKPNMKETAQNNA 343
[Close]
BLAST of SMED30031190 vs. TrEMBL
Match: A0A183WJM2 (Uncharacterized protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS12325 PE=4 SV=1)

HSP 1 Score: 389.037 bits (998), Expect = 8.733e-122
Identity = 185/317 (58.36%), Postives = 244/317 (76.97%), Query Frame = 1 
Query:   40 GDQLPVYPPNIQGELRLVLQNKDLFLLCYKYLEDYRRLNEEQNRIITKDFIESQIKIGDEEIDELQTELTNAEYKLTVRTRHNETLLGQLSVRGMSTLGDGSPRRNI--CRSPQIRK---CPHMSSSIDMKS-----KDLTLFENIALAIEEIQREIKNKMKPLCDFYRDICYLQLHFVLVLTQICNYCMPLVTENVGLIDRVFFWSDRPEFELVRKSGEIADRLKDIFTRTQSVLTLLVENVMFLQNKVELQKNLERKFQDRLEVQQLEKDKVLRKIHGSRLDMDLLEEENDRLVGILVKLDPKYVESDHFNELAD 960
             DQLP YP NIQGELRL+LQ KD+FL+CYK+LE+YRRL  EQNR+ITK+F++ Q++IG++EI EL+ EL  AE +L  R RHNE L  QL+  G ++L  G+  R++  CRSPQ+RK         S+  +          + +NIALAI EI +E++ K+KPL D+YRD+ YLQLHF LVL Q+CNYCMPLVTEN+GLIDR+FFWS+RPEF+LVR++G+I DRLK+I+TR Q+VL +L E+V FLQ+KVELQK LE++F +RL+ QQ  KDK+LR +H ++ +MDLLEEENDRLVG LVK+DP +V+S HF ELA+
Sbjct:    7 SDQLPTYPNNIQGELRLILQTKDIFLVCYKHLEEYRRLVNEQNRLITKEFVQEQLQIGEDEIAELKIELQEAENRLGGRLRHNELLADQLASLGYASLESGASVRHLALCRSPQMRKNMLAQRQGGSLGFQKYSEPRPPTHMIDNIALAIAEIHQEVRAKVKPLLDYYRDLFYLQLHFSLVLGQVCNYCMPLVTENIGLIDRIFFWSERPEFDLVRRAGDIGDRLKEIYTRAQTVLGVLAESVRFLQSKVELQKTLEQRFHERLDEQQKTKDKILRDVHATKQNMDLLEEENDRLVGSLVKVDPSFVDSRHFYELAE 323
[Close]
BLAST of SMED30031190 vs. TrEMBL
Match: A0A5J4N8Q9 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0002589 PE=4 SV=1)

HSP 1 Score: 386.341 bits (991), Expect = 1.198e-120
Identity = 182/317 (57.41%), Postives = 242/317 (76.34%), Query Frame = 1 
Query:   43 DQLPVYPPNIQGELRLVLQNKDLFLLCYKYLEDYRRLNEEQNRIITKDFIESQIKIGDEEIDELQTELTNAEYKLTVRTRHNETLLGQLSVRGMSTLGDGSPRRNI--CRSPQIRKCPHMSSSIDMKSKDLT---------LFENIALAIEEIQREIKNKMKPLCDFYRDICYLQLHFVLVLTQICNYCMPLVTENVGLIDRVFFWSDRPEFELVRKSGEIADRLKDIFTRTQSVLTLLVENVMFLQNKVELQKNLERKFQDRLEVQQLEKDKVLRKIHGSRLDMDLLEEENDRLVGILVKLDPKYVESDHFNELAD 960
            DQLP YP NIQGELRL+LQ KD+FL+CYK+LE+YRRL  EQNR+ITK+F++ Q++IG++EI EL+ EL +AE +L  R RHNE L   L+  G S+L  G+  R++  CRSPQ+RK                         + +NIALAI EI +E++ K+KPL D+YRD+ YLQLHF LVL Q+CNYCMPLVTEN+GLIDR+FFWS+RPEF+LVR++G+I DRLK+I+TR Q+VL +L E+V FLQ+KV+LQK LE++F +RL+ QQ  KDK+LR++H ++ +MD+LEEENDRLVG LVK+DP +V+S HF ELA+
Sbjct:    8 DQLPTYPNNIQGELRLILQTKDVFLVCYKHLEEYRRLVNEQNRLITKEFVQEQLQIGEDEISELKIELQDAEARLNGRLRHNELLADHLASLGYSSLESGASVRHLALCRSPQMRKSMLAQRQGGGGLGSSRLTEPRPPTHMIDNIALAIAEIHQEVRAKVKPLLDYYRDLFYLQLHFSLVLGQVCNYCMPLVTENIGLIDRIFFWSERPEFDLVRRAGDIGDRLKEIYTRAQTVLGVLAESVRFLQSKVDLQKTLEQRFHERLDEQQKTKDKILREVHTTKQNMDMLEEENDRLVGSLVKVDPSFVDSRHFYELAE 324
[Close]
BLAST of SMED30031190 vs. Planmine SMEST
Match: SMESG000021217.1 (SMESG000021217.1)

HSP 1 Score: 1090.49 bits (2819), Expect = 0.000e+0
Identity = 550/550 (100.00%), Postives = 550/550 (100.00%), Query Frame = 1 
Query:   34 MQGDQLPVYPPNIQGELRLVLQNKDLFLLCYKYLEDYRRLNEEQNRIITKDFIESQIKIGDEEIDELQTELTNAEYKLTVRTRHNETLLGQLSVRGMSTLGDGSPRRNICRSPQIRKCPHMSSSIDMKSKDLTLFENIALAIEEIQREIKNKMKPLCDFYRDICYLQLHFVLVLTQICNYCMPLVTENVGLIDRVFFWSDRPEFELVRKSGEIADRLKDIFTRTQSVLTLLVENVMFLQNKVELQKNLERKFQDRLEVQQLEKDKVLRKIHGSRLDMDLLEEENDRLVGILVKLDPKYVESDHFNELADYSVRRHYLEGRLTPNGFKAHQTNVTPPPGGHSLNQNQILRGSSNSEPSKNETLSLNTHNNSLFTNINVSNIDKIESGXXXXXXXXXXXXXXXXXSYEKEMAPINSRKHGHKGVHTFTDESNLLESNKHDIGKLSIRDKLLTKTKSIFDSAFKKSIDQKQRNNYVKVFEGEILAELSNFNSTEQTPNSHLPEGEHETNSWDFSLERSLKRKYDLKESELHEKKPTRLKTHHRKKDKNLLMET 1683
            MQGDQLPVYPPNIQGELRLVLQNKDLFLLCYKYLEDYRRLNEEQNRIITKDFIESQIKIGDEEIDELQTELTNAEYKLTVRTRHNETLLGQLSVRGMSTLGDGSPRRNICRSPQIRKCPHMSSSIDMKSKDLTLFENIALAIEEIQREIKNKMKPLCDFYRDICYLQLHFVLVLTQICNYCMPLVTENVGLIDRVFFWSDRPEFELVRKSGEIADRLKDIFTRTQSVLTLLVENVMFLQNKVELQKNLERKFQDRLEVQQLEKDKVLRKIHGSRLDMDLLEEENDRLVGILVKLDPKYVESDHFNELADYSVRRHYLEGRLTPNGFKAHQTNVTPPPGGHSLNQNQILRGSSNSEPSKNETLSLNTHNNSLFTNINVSNIDKIESGKKIKTRKKKRDKKRVDKSYEKEMAPINSRKHGHKGVHTFTDESNLLESNKHDIGKLSIRDKLLTKTKSIFDSAFKKSIDQKQRNNYVKVFEGEILAELSNFNSTEQTPNSHLPEGEHETNSWDFSLERSLKRKYDLKESELHEKKPTRLKTHHRKKDKNLLMET
Sbjct:    1 MQGDQLPVYPPNIQGELRLVLQNKDLFLLCYKYLEDYRRLNEEQNRIITKDFIESQIKIGDEEIDELQTELTNAEYKLTVRTRHNETLLGQLSVRGMSTLGDGSPRRNICRSPQIRKCPHMSSSIDMKSKDLTLFENIALAIEEIQREIKNKMKPLCDFYRDICYLQLHFVLVLTQICNYCMPLVTENVGLIDRVFFWSDRPEFELVRKSGEIADRLKDIFTRTQSVLTLLVENVMFLQNKVELQKNLERKFQDRLEVQQLEKDKVLRKIHGSRLDMDLLEEENDRLVGILVKLDPKYVESDHFNELADYSVRRHYLEGRLTPNGFKAHQTNVTPPPGGHSLNQNQILRGSSNSEPSKNETLSLNTHNNSLFTNINVSNIDKIESGKKIKTRKKKRDKKRVDKSYEKEMAPINSRKHGHKGVHTFTDESNLLESNKHDIGKLSIRDKLLTKTKSIFDSAFKKSIDQKQRNNYVKVFEGEILAELSNFNSTEQTPNSHLPEGEHETNSWDFSLERSLKRKYDLKESELHEKKPTRLKTHHRKKDKNLLMET 550
[Close]
BLAST of SMED30031190 vs. Planmine SMEST
Match: SMESG000072312.1 (SMESG000072312.1)

HSP 1 Score: 320.857 bits (821), Expect = 1.484e-102
Identity = 175/384 (45.57%), Postives = 255/384 (66.41%), Query Frame = 1 
Query:   43 DQLPVYPPNIQGELRLVLQNKDLFLLCYKYLEDYRRLNEEQNRIITKDFIESQIKIGDEEIDELQTELTNAEYKLTVRTRHNETLLGQLSVRGMSTLGDGSPRR-NICRSPQIRKCPHMSSSIDMKSKDLT---LFENIALAIEEIQREIKNKMKPLCDFYRDICYLQLHFVLVLTQICNYCMPLVTENVGLIDRVFFWSDRPEFELVRKSGEIADRLKDIFTRTQSVLTLLVENVMFLQNKVELQKNLERKFQDRLEVQQLEKDKVLRKIHGSRLDMDLLEEENDRLVGILVKLDPKYVESDHFNELADYSVRRHYLEGRLTPNGFK--AHQTNVTPPPGGHSLNQN--QILRGSSNSEPSKNETLSLNTHNNSLFTNINVSN 1170
            D +PVYP +IQGELR++LQ KD FLLC+K+LE+ R L ++QNRIITK+FI  QIK G+ EI++L+TEL++ +  L+VR RHN+ L+ +L       + + S RR   CRS   R C    + I   +KD T   + ENIA  I+  Q E+K  ++PL +FY+D+ YLQLHF L LTQI  YCMPL+T+++ LIDR+FFW++RPEFEL++KSG+I DRL+DI+TR Q +L  L+++V+FLQ++++LQ+ LE KF D++      KD +L +IH +RL++DLLEEENDRLVG LVK+DP +VE + F   A+     H     L PN ++  +  ++++      S  ++  ++ R  S +  S N   + NT N     + NVSN
Sbjct:   10 DFMPVYPSSIQGELRIILQVKDEFLLCFKHLENLRNLWDQQNRIITKEFIIEQIKTGEMEIEQLETELSSIKEVLSVRERHNDALINELRNNNEKYVAESSHRRVKSCRSSMFRACHKNLNEIGDSAKDNTFSSVLENIANRIDAAQIELKENLRPLLEFYKDLFYLQLHFTLALTQISCYCMPLMTDSISLIDRLFFWTERPEFELIKKSGQICDRLRDIYTRLQRILGRLLDSVLFLQDQIDLQRLLEIKFYDKIRQMNQMKDNLLEEIHTTRLNLDLLEEENDRLVGTLVKIDPLFVEREAFGLYAE-----HKNAPELNPNEYQKSSRPSDISQIRNLSSKRKSKVKVARIGSETAISDNANNTFNTSN----VHFNVSN 384
[Close]
BLAST of SMED30031190 vs. Planmine SMEST
Match: SMESG000041156.1 (SMESG000041156.1)

HSP 1 Score: 121.709 bits (304), Expect = 3.137e-29
Identity = 92/302 (30.46%), Postives = 153/302 (50.66%), Query Frame = 1 
Query:   34 MQGDQLPVYPPNIQGELRLVLQNKDLFLLCYKYLEDYRRLNEEQNRIITKDFIESQIKIGDEEIDELQTELTNAEYKLTVRTRHNETLL---GQLSVRGMSTLGDGSP--RRNICRSPQIRKCPH---MSSSIDM----KSKDLTLFENIALAIEEIQREIKNKMKPLCDFYRDICYLQLHFVLVLTQICNYCMPLVTENVGLIDRVFFWSDRPEFELVRKSGEIADRLKDIFTRTQSVLTLLVENVMFLQNKVELQKNLERKFQDRLEVQQLEKDKVLRKIHGSRLDMDLLEEENDRLVGI 903
            M    LP+YP +++G +R +L  KD F+LC + LE Y R      R + +D+I +++++G + I+ L+ ELT    +L       +TL+    +L  RG S L    P    ++C   +  K        S ID+    + K+L L E       E Q     K+K + +F +++  +QLHF +VL Q  NY +PL+      +DR+ +W  RPEF ++ K    AD+L+DI    Q ++  LV   M ++ K    + LE+ F   ++ Q  EK  +L KI   +   +LL+ E+ R++G 
Sbjct:    1 MFDSSLPIYPSDVRGTVRHILILKDFFILCSELLERYCRFGSLAGRQLNRDYILTEVQVGYDTIELLKGELTE---QLQRNMDMEKTLVEMRKELIKRG-SVLNCERPIVVESLCHQLKYHKMKMKLGTDSCIDIYYNKEDKELNLPEQF-----ECQ-----KVKTVLEFLQELYLIQLHFHIVLVQGLNYALPLLINGHNFLDRLLYWPMRPEFGMIHKKINFADKLRDIQVELQRLVEELVSGCMVVEAKFLYDQKLEKYFNRTIKDQCNEKQLLLEKIQHEKEMFELLQVEHSRIIGF 288
[Close]
BLAST of SMED30031190 vs. Planmine SMEST
Match: SMESG000013708.1 (SMESG000013708.1)

HSP 1 Score: 58.151 bits (139), Expect = 1.603e-8
Identity = 42/147 (28.57%), Postives = 81/147 (55.10%), Query Frame = 1 
Query:  493 KPLCDFYRDICYLQLHFVLVLTQICNYCMPLVTENVGLIDRVFFWSDRPEFELVRKSGEIADRLKDIFTRTQSVLTLLVENVMFLQNKVELQKNLERKFQDRLEVQQLEKDKVLRKIHGSRLDMDLLEEENDRLVGILVKLDPKYVE 933
            +P  +FY D+ +LQLHF L+L Q CN+ +PL+T  +  I R+ +W  +P +    KS  + D+L  I     S L  L+  +  ++  +   +N  + +   ++    +K ++L +++  R   + L  E++RLVG ++K+D K+ +
Sbjct:   96 RPPIEFYEDLMHLQLHFHLLLIQGCNFALPLLTTKLSFISRIIYWPSKPLYGFENKSKRLRDKLIKIKLLLSSRLEELINAIGEVEKWILFYQNSHQVYGTLMKAACDKKKEILFEMNNLRSTHNNLILEHNRLVGAILKIDAKFFK 242
The following BLAST results are available for this feature:
BLAST of SMED30031190 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031190 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031190 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031190 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031190 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031190 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031190 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031190 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A504Z4S61.082e-12356.05Uncharacterized protein OS=Fasciola gigantica OX=4... [more]
A0A183ACA61.104e-12358.41Uncharacterized protein OS=Echinostoma caproni OX=... [more]
A0A2H1CTU21.944e-12356.05Uncharacterized protein OS=Fasciola hepatica OX=61... [more]
A0A183WJM28.733e-12258.36Uncharacterized protein OS=Trichobilharzia regenti... [more]
A0A5J4N8Q91.198e-12057.41Uncharacterized protein OS=Paragonimus westermani ... [more]
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BLAST of SMED30031190 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031190 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031190 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031190 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031190 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031190 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000021217.10.000e+0100.00SMESG000021217.1[more]
SMESG000072312.11.484e-10245.57SMESG000072312.1[more]
SMESG000041156.13.137e-2930.46SMESG000041156.1[more]
SMESG000013708.11.603e-828.57SMESG000013708.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031190 ID=SMED30031190|Name=SMED30031190|organism=Schmidtea mediterranea sexual|type=transcript|length=1891bp
TTTATTAACTGCTTCTCGATTTTGATTATATAAATGCAAGGTGATCAACT
TCCGGTTTACCCGCCAAATATTCAAGGTGAATTGAGATTGGTTTTGCAAA
ATAAAGATTTGTTTTTACTATGCTATAAATATTTGGAGGATTATCGTAGA
TTAAATGAAGAACAAAATAGAATAATTACAAAAGATTTCATTGAGTCTCA
AATAAAAATTGGGGATGAAGAGATCGATGAGTTACAAACAGAACTGACAA
ATGCTGAATACAAATTAACTGTGAGAACTCGTCACAATGAAACTCTACTT
GGGCAACTTTCGGTTCGTGGAATGTCAACTCTCGGTGATGGTTCACCTCG
TCGTAATATCTGTAGATCTCCCCAAATTAGAAAATGTCCTCACATGTCAT
CCTCTATTGATATGAAGTCTAAGGATTTAACTTTGTTTGAAAATATTGCT
CTTGCGATTGAAGAAATTCAAAGAGAAATTAAAAATAAAATGAAACCCCT
CTGTGACTTTTATCGAGATATTTGCTATCTTCAACTACATTTCGTTTTGG
TTCTGACCCAAATCTGCAACTATTGTATGCCTTTGGTCACCGAAAATGTT
GGTTTGATTGACCGAGTATTCTTTTGGTCTGATCGGCCAGAATTTGAACT
AGTAAGAAAGTCTGGTGAAATAGCTGATCGGTTAAAGGACATTTTTACAA
GAACTCAAAGTGTTCTAACATTACTTGTGGAAAACGTCATGTTTTTACAA
AATAAAGTCGAACTTCAAAAGAATCTCGAAAGAAAATTTCAAGATCGTTT
AGAGGTGCAGCAATTAGAAAAGGACAAAGTTTTACGAAAAATACATGGCT
CAAGGTTGGATATGGATTTGTTAGAGGAAGAAAATGATCGTCTTGTTGGC
ATATTGGTAAAGCTAGATCCGAAATATGTTGAATCGGACCATTTTAATGA
GTTAGCAGATTATTCAGTCCGCAGACATTATCTTGAAGGTAGATTAACAC
CGAATGGGTTTAAAGCCCACCAAACTAATGTTACTCCACCACCTGGAGGA
CATTCTTTAAATCAAAACCAGATTTTAAGGGGGAGTTCAAATTCAGAACC
ATCCAAAAACGAAACGCTTTCCCTAAATACCCACAACAATAGTTTATTCA
CAAATATTAATGTTAGTAATATTGATAAAATTGAAAGTGGGAAAAAAATC
AAAACCCGCAAAAAGAAACGTGATAAGAAACGAGTTGACAAAAGTTATGA
GAAAGAAATGGCACCGATTAACAGCAGAAAACATGGACATAAAGGAGTCC
ATACATTTACCGATGAATCAAACTTGTTGGAATCAAACAAACATGATATC
GGAAAACTATCCATAAGGGACAAACTGCTTACGAAAACGAAAAGCATTTT
CGACAGTGCATTCAAAAAGTCAATTGATCAAAAACAAAGAAACAATTATG
TTAAAGTTTTCGAAGGTGAAATTTTGGCGGAATTATCGAATTTCAATTCA
ACTGAACAAACGCCAAATTCACATTTGCCTGAAGGTGAGCACGAAACTAA
TTCCTGGGATTTTTCTCTTGAAAGAAGCTTGAAACGAAAATATGATTTGA
AAGAAAGCGAATTGCACGAGAAAAAGCCCACCCGACTAAAGACCCACCAT
AGAAAGAAAGACAAAAACTTACTAATGGAGACCTGAATAAAAAATATTAA
TTCATTGAAAATGAATTAAAATTCGAAATAGTTGAAATTTAACAAAACTA
TTTTGCCTTTAAATCACGTTTATTTAAAGATGATTTTACACCTGAATAAA
ATTAGTTATTTTTTCTCATTAAAGCATTACAAGTACTGGTTGTCATGCAA
CCAGAAATATAATAAAATTAAAACAACAAATATTATTGCAC
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protein sequence of SMED30031190-orf-1

>SMED30031190-orf-1 ID=SMED30031190-orf-1|Name=SMED30031190-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=551bp
MQGDQLPVYPPNIQGELRLVLQNKDLFLLCYKYLEDYRRLNEEQNRIITK
DFIESQIKIGDEEIDELQTELTNAEYKLTVRTRHNETLLGQLSVRGMSTL
GDGSPRRNICRSPQIRKCPHMSSSIDMKSKDLTLFENIALAIEEIQREIK
NKMKPLCDFYRDICYLQLHFVLVLTQICNYCMPLVTENVGLIDRVFFWSD
RPEFELVRKSGEIADRLKDIFTRTQSVLTLLVENVMFLQNKVELQKNLER
KFQDRLEVQQLEKDKVLRKIHGSRLDMDLLEEENDRLVGILVKLDPKYVE
SDHFNELADYSVRRHYLEGRLTPNGFKAHQTNVTPPPGGHSLNQNQILRG
SSNSEPSKNETLSLNTHNNSLFTNINVSNIDKIESGKKIKTRKKKRDKKR
VDKSYEKEMAPINSRKHGHKGVHTFTDESNLLESNKHDIGKLSIRDKLLT
KTKSIFDSAFKKSIDQKQRNNYVKVFEGEILAELSNFNSTEQTPNSHLPE
GEHETNSWDFSLERSLKRKYDLKESELHEKKPTRLKTHHRKKDKNLLMET
*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 50..77
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 386..405
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 523..550