G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30031189
Length (bp)1165
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30031189) t-SNE clustered cells

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30031189) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30031189) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30031189) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30031189

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30031189SMESG000063918.1 dd_Smed_v4_19017_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0R3UKE8 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS8061 PE=4 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 1.047e-26
Identity = 95/293 (32.42%), Postives = 152/293 (51.88%), Query Frame = 1
Query:  166 GRSHNMRYTSFYHAIIEL-VLDLWIFTDKISMFAHLPDDKYV-------SAMIFSTLTFWSINALLCSRNWMVMLVALARLEAIFRPLKIR-SYSIFSSCKSRLYTXXXXXXXXXXXXXXXXYKKAIVCENENKIIFDSHWKNSSLYQCFEIGYFIYQSVFPVVIVTISTLTMIYFLFKQ--TFKSTHLLKQYFRENETQCMTS-------------QQKATLTITVLAGVFTVTEIPIFIITLLKFTYPNGLNNLMVLYQITRALVVVDSCCNFLIYVSTSEHYRRDLWYLF 972
            GRS  +R+ S   +++ L V+++W+ T   S+F H   +++        S   FS   F  +N+ +CSRNW+V L+ALAR EAI RP+  R S  IFS  +  +YT  +++  F LS +R+ +++ +VC N N  +       + +    E  +F YQS  P+ IVTISTL MI  L +    F+    ++Q    +E  C+ S             Q +AT  I ++A VF V E P+F   +     P   N ++ +++  R L+V DS  NF+IY+ T + +R +L  L 
Sbjct:   70 GRSSAVRFGSMRKSLVTLTVIEMWLNT---SIFVHKAWEEFATMRHETFSEFCFSFFLFSILNSAICSRNWVVTLIALARCEAITRPVASRVSTHIFSPKRQLMYTATLIICGFVLSTLRLVFRQMLVCTNLNNAVVQVEANRTWVQDVSEKVFFAYQSAIPITIVTISTLFMIVILLRHRMPFEKKGSIRQAQCRDERSCVVSSLHEVRQAQRLPNQIRATRFILLIATVFIVCEAPVFFAVVFVEAIPR--NAVIGIFKYLRFLIVADSFANFVIYLLTIKPFRLELVRLL 357          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A183SJQ7 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS4455 PE=4 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 1.828e-24
Identity = 91/299 (30.43%), Postives = 150/299 (50.17%), Query Frame = 1
Query:  166 GRSHNMRYTSFYHAIIELV-LDLWIFTDKISMFAHL-------PDDKYVSAMIFSTLTFWSINALLCSRNWMVMLVALARLEAIFRPLKIR-SYSIFSSCKSRLYTXXXXXXXXXXXXXXXXYKKAIVCENENKIIFDSHWKNSSLYQCFEIGYFIYQSVFPVVIVTISTLTMIYFLFKQTFKST-------------------------HLLKQYFRENETQCMTSQQKATLTITVLAGVFTVTEIPIFIITLLKFTYPNGLNNLMVLYQITRALVVVDSCCNFLIYVSTSEHYRRDL 960
             R+   R+ S   ++I L  +++W+ T   S+F H        P  + + A I + ++F  +N+ +CSRNW V L+AL+R EAI RPL  R S  I S  +  +  + V+ L+   S IR+ +   +VC N    +      NS+  Q FE  +F YQS  P+ IV ++T+ MI+ L +   +S                           +L + + +     +T+Q +AT TI  +A VFT+ E P+F I +  F    G+    V+ Q+ + L+++DS  N +IY+ TSEH+R +L
Sbjct:   46 ARNGAKRFGSMRKSLIALTSIEMWLNT---SIFVHKVWEDYGSPHPETLLAYILAAISFSLLNSAICSRNWCVTLIALSRCEAITRPLAHRSSKQILSPNRQIILLVCVVTLAMVFSAIRLTFLSIVVCTNLEHKVIRLPPTNSTNAQIFEKVFFAYQSAIPISIVALATVFMIWTLLRPRIESAKKSPSAKAQPQLQRQSTVSGTNNHGKILPRGYEKKNAPRLTNQVRATRTIFFIAAVFTILEAPVFFIIV--FENHIGIEVKKVIVQLLKFLIILDSYTNIVIYLLTSEHFRTEL 339          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A3P7M935 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS14106 PE=4 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 2.142e-22
Identity = 93/301 (30.90%), Postives = 152/301 (50.50%), Query Frame = 1
Query:  166 GRSHNMRYTSFYHAIIELV-LDLWIFTDKISMFAHL-------PDDKYVSAMIFSTLTFWSINALLCSRNWMVMLVALARLEAIFRPLKIR-SYSIFSSCKSRLYTXXXXXXXXXXXXXXXXYKKAIVCENENKIIFDSHWKNSSLYQCFEIGYFIYQSVFPVVIVTISTLTMIYFLF-------KQTFKSTHLLKQ--------------------YFRENETQCMTSQQKATLTITVLAGVFTVTEIPIFIITLLKFTYPNGLNNLMVLYQITRALVVVDSCCNFLIYVSTSEHYRRDL 960
             R    R+ S   ++I L  +++W+ T   S+F H        P  + + + I + + F  +N+ +CSRNW V L+AL+R EAI RPL  R S  + S  +  +  + V++L    S +R+ +   +VC N N  +      NS+    FE  +F YQS  P+ IV ++T+ MI+ L        K+T  S  + +Q                    Y + N ++ +T+Q +AT TI  +A VFTV E P+F I +  F    G +  +V+ Q+ + L+++DS  N  IY+ TSEH+R +L
Sbjct:   46 ARHRAKRFGSMRKSLIALTSIEMWLNT---SIFVHKVWEDYGSPHPETLLSYILAAILFSLLNSAICSRNWCVTLIALSRCEAITRPLAHRSSKQLLSPNRQLILLVFVIILGIIFSAVRLTFLNIVVCTNLNNTVIRLPPANSTNAGRFEKVFFAYQSAIPISIVALATVFMIWTLLRPKIAAAKRTPSSPEIQRQLQCPSTAHGSHNHGKILPRGYEKRNVSR-LTNQVRATRTIFFIAAVFTVLEAPVFFIIV--FENHIGSDVRVVIVQLLKFLIILDSYTNIAIYLLTSEHFRTEL 340          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0R3T1S6 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS843 PE=4 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 8.153e-20
Identity = 86/295 (29.15%), Postives = 142/295 (48.14%), Query Frame = 1
Query:  175 HNMRYTSFYHAIIELV-LDLW----IFTDKISMFAHLPDDKYVSAMIFSTLTFWSINALLCSRNWMVMLVALARLEAIFRPLKIR-SYSIFSSCKSRLYTXXXXXXXXXXXXXXXXYKKAIVCENENKIIFDSHWKNSSLYQCFEIGYFIYQSVFPVVIVTISTLTMIYFLFKQTFKSTHLLKQYFRENETQCMTSQQK----------------------ATLTITVLAGVFTVTEIPIFIITLL-KFTYPNGLNNLMVLYQITRALVVVDSCCNFLIYVSTSEHYRRDLWYLF 972
            H+ R+ S   ++I L  +++W    IF +K      +P  +  +  I S L F  +N  +CSRNW V L+A AR E I RP+  R +  IFS  +  +Y  I++ +   LS +R+ ++  IVC N + ++       + L    E  +F YQS  P+ +V+++T+ M+  L + +        Q  R+N ++   S  K                      AT  I ++ G+F V E PIF   +L K   P      + +Y+  R L+V D+  NF+IY+ TS+H+R  L  +F
Sbjct:   53 HSRRFGSLRKSVIVLTSIEMWLNCSIFINKAWEGFGMPKHRTFAGFICSFLLFGLLNNAICSRNWAVTLIAFARCEVITRPVAHRVNTHIFSPNRQIIYISILIFVGMVLSFLRLLFRPVIVCTNLDGLLQYPEINVTQLQIINEKVFFAYQSAIPIFLVSLATIFMMITLLRSS--------QIVRKNSSEPKNSDGKIISCVYQQKKKKKSYHLSKHVRATRAILLIIGIFIVCEAPIFFAVVLEKHISPEA---KLTVYKALRFLIVTDTYANFIIYLLTSKHFRYGLKKVF 336          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A087VX96 (Rhodopsin orphan GPCR OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000066200 PE=4 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 2.926e-18
Identity = 88/320 (27.50%), Postives = 149/320 (46.56%), Query Frame = 1
Query:  169 RSHNMRYTSFYHAIIEL-VLDLWIFTDKISMFAHLPDDKY-------VSAMIFSTLTFWSINALLCSRNWMVMLVALARLEAIFRPLKIR-SYSIFSSCKSRLYTXXXXXXXXXXXXXXXXYKKAIVCENENKIIFDSHWKNSSLYQCF-EIGYFIYQSVFPVVIVTISTLTMIYFLFKQTFKSTHLLKQY--------------------FREN--------------------ETQCMTSQQKATLTITVLAGVFTVTEIPIFIITLLKFTYPNGLNNLMVLYQIT--RALVVVDSCCNFLIYVSTSEHYRRDLWYLF 972
            R    R+ S   +++ L  +++W+ T   ++F H   ++Y       +S   FS + F  +N+ +CSRNW+V L+ALAR EAI RP+  R S  IFS  +  +Y  ++++   +LS  R+  ++ ++C N + ++       +S  Q   E  +F YQS  P+ +VTI+TL MI  L +    S    +Q                     FR+                     + Q + +Q +AT  I  +A +F V E P+F   +      N ++  +V+   T  R L++ DS  NF+IY+ TS  +R +L  L 
Sbjct:   83 RPAATRFGSMQRSLVALTAMEMWLNT---TIFVHKAWEEYFTTRHERLSEFCFSFVLFSGLNSAICSRNWVVTLIALARCEAITRPVATRVSTHIFSPQRQLIYIGVLIVCGIALSTFRLVIRQVLICTNLDNVVLHIRSDQTSWLQLISEKIFFAYQSAIPITVVTIATLFMIVVLLRHRMPSERRKEQQRKVEVRETQKEGEKWKRWRCFRDGRLTPRDDGGGCVVPSLQAVRQAQRLPNQIRATRFILFIATIFIVCEAPVFFAVICA----NSMSPTVVIRVFTYLRFLIIADSFANFVIYLLTSRPFRVELVNLL 395          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Match: EDO48802 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RIF2])

HSP 1 Score: 48.9062 bits (115), Expect = 1.524e-6
Identity = 37/137 (27.01%), Postives = 65/137 (47.45%), Query Frame = 1
Query:  559 WKNSSLYQC-FEIGYFIYQSVFPVVIVTISTLTMIYFLFKQTFKSTHLLKQYFRENETQCMTSQQKATLTITVLAGVFTVTEIPIFIITLLKFTYPNGLNN--LMVLYQITRALVVVDSCCNFLIYVSTSEHYRRDL 960
            W  S LYQ  + +G F  + V P + +T++  T I    K+T K       Y +          +K T  +T L  ++++  +P  I  L+     + L +  + + Y+ TR L + +SC N L+Y + S+ +R DL
Sbjct:  170 WGGSELYQINYVLGVFTPEFVIPAIAITLA-YTRIVCHLKKTHKKNSQHGLYQKSTMAARTRQNKKTTKVLTGLVTIYSICILPHMICVLMVIFNKSYLGSFAMRLAYEFTRLLTITNSCLNPLLYGAISKSFRTDL 305          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000063918.1 (SMESG000063918.1)

HSP 1 Score: 667.922 bits (1722), Expect = 0.000e+0
Identity = 356/359 (99.16%), Postives = 357/359 (99.44%), Query Frame = 1
Query:    1 RNC*NVYYFQKNLNFSSNEYELFDYSTNXXXXXXXXXXXXTNTLTVFTLSQPRFKGRSHNMRYTSFYHAIIELVLDLWIFTDKISMFAHLPDDKYVSAMIFSTLTFWSINALLCSRNWMVMLVALARLEAIFRPLKIRSYSIFSSCKSRLYTXXXXXXXXXXXXXXXXYKKAIVCENENKIIFDSHWKNSSLYQCFEIGYFIYQSVFPVVIVTISTLTMIYFLFKQTFKSTHLLKQYFRENETQCMTSQQKATLTITVLAGVFTVTEIPIFIITLLKFTYPNGLNNLMVLYQITRALVVVDSCCNFLIYVSTSEHYRRDLWYLFQRIFRWHKMFVNEQEESFGMSRIVSYIPNSRRHKV 1077
            RNC NVYYF KNLNFSSNEYELFDYSTNIFIAVVAIIGFVTNTLTVFTLSQPRFKGRSHNMRYTSFYHAIIELVLDLWIFTDKISMFAHLPDDKYVSAMIFSTLTFWSINALLCSRNWMVMLVALARLEAIFRPLKIRSYSIFSSCKSRLYTIIVLLLSFSLSVIRVFYKKAIVCENENKIIFDSHWKNSSLYQCFEIGYFIYQSVFPVVIVTISTLTMIYFLFKQTFKSTHLLKQYFRENETQCMTSQQKATLTITVLAGVFTVTEIPIFIITLLKFTYPNGL+NLMVLYQITRALVVVDSCCNFLIYVSTSEHYRRDLWYLFQRIFRWHKMFVNEQEESFGMSRIVSYIPNSRRHKV
Sbjct:    8 RNCSNVYYFHKNLNFSSNEYELFDYSTNIFIAVVAIIGFVTNTLTVFTLSQPRFKGRSHNMRYTSFYHAIIELVLDLWIFTDKISMFAHLPDDKYVSAMIFSTLTFWSINALLCSRNWMVMLVALARLEAIFRPLKIRSYSIFSSCKSRLYTIIVLLLSFSLSVIRVFYKKAIVCENENKIIFDSHWKNSSLYQCFEIGYFIYQSVFPVVIVTISTLTMIYFLFKQTFKSTHLLKQYFRENETQCMTSQQKATLTITVLAGVFTVTEIPIFIITLLKFTYPNGLSNLMVLYQITRALVVVDSCCNFLIYVSTSEHYRRDLWYLFQRIFRWHKMFVNEQEESFGMSRIVSYIPNSRRHKV 366          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000078733.1 (SMESG000078733.1)

HSP 1 Score: 129.413 bits (324), Expect = 8.370e-34
Identity = 101/296 (34.12%), Postives = 163/296 (55.07%), Query Frame = 1
Query:  121 TNTLTVFTLSQPRFKGRSHNMRYTSFYHAIIELVLDLWIFTDKISMFAHLPDDKYVSAMIFSTLTFWSINALLCSRNWMVMLVALARLEAIFRPLKIRSYSIFSSCKSRLYTXXXXXXXXXXXXXXXXYKKAIVCENENKIIFDSHWKNSSLYQCFEI-GYFIYQSVFPVVIVTISTLTMIYFLFKQT------FKSTHLLKQYFRENETQCMTSQQKATLTITVLAGVFTVTEIPIFIITLL--KFTYPNGLN-NLMVLYQITRALVVVDSCCNFLIYVSTSEHYRRDLWYLFQR 978
             N ++V  LS+P    R   +R      +I E  L+ +I    ++   ++     + A+   T+ FW+I +L+C+RNW+V+L+A +R E +F PL   S   F++   R  +I++ ++S+ LS +R+F KKAIVC N +++    H      Y  +E   +FI+QSV PV++V   +  M Y L K+        ++  L ++ + +      +SQ+KAT TI VLA VFT  E P+FII +L    T+   LN N  V+Y I   LVV+DSC NF++Y+ TS+ +R +   L  R
Sbjct:   40 ANGVSVVILSRPNSSQRFGQIRLNLLILSISETSLNAFITVFNLTHIVNISQYSRIGALWIDTMLFWNITSLICNRNWIVILIACSRCEIVFLPLHSVSGLKFNNKVIRNLSILIFIISYLLSSVRLFSKKAIVCTNIDEVKLQPHLSGQWAYSVYETYCFFIFQSVLPVLVVGGISAGMTYSLIKEPNPQLLRPQNNTLSQKSYSKTSNIRGSSQRKATKTIFVLAIVFTTLEAPVFIIQILIQNGTFAFILNSNPHVVYFIINFLVVLDSCSNFMVYLVTSDKFRAEFRRLIWR 335          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000031763.1 (SMESG000031763.1)

HSP 1 Score: 128.257 bits (321), Expect = 2.688e-33
Identity = 111/316 (35.13%), Postives = 168/316 (53.16%), Query Frame = 1
Query:  124 NTLTVFTLSQPRFKGRSHNMRYTSFYHAIIELVLDLWIFTDKISMFAHLPDDKYVSAMIFSTLTFWSINALLCSRNWMVMLVALARLEAIFRPLKIRSYSIFSSCKSRLYTXXXXXXXXXXXXXXXXYKKAIVCENENKIIFDSHWKNSSLYQCFEI-GYFIYQSVFPVVIVTISTLTMIYFLFKQTFKSTHLLK----QYFRENETQCMTSQQKATLTITVLAGVFTVTEIPIFIITLL--KFTYPNGLNNLMVLYQI---TRALVVVDSCCNFLIYVSTSEHYRRDLWYLFQRIFRWHKMFVNEQEESFGMSRI 1041
            N ++V  LS+     R   +R      +I E  L+  I    I+  + L     V+A++F T  FW+I  L+C+RNW+V+L+AL+R E +F PL       F++   +L ++I+LLLS+ LS +R+F KKAIVC N +KI+ + H   S  Y  +E   +FIYQSV PV++V   +L M   L +   +     K    Q   + +T    SQ+KAT TI VLA VFT  E P+FII  L        G NN  +  Q+      LVV DSCCN  +Y+ TS+ +R ++  L   +F +        ++SF + ++
Sbjct:   39 NGISVIILSRSNASHRFGPIRLNLLILSISETALNSLIAVFNIASESELDKLFSVTALLFDTFVFWNILTLICTRNWIVVLIALSRCEVVFLPLNSVRGLTFNNSVIKLCSVIILLLSYLLSFVRLFSKKAIVCSNLSKILLEPHLYKSWQYVIYETYCFFIYQSVLPVILVAGVSLAMFVSLIRGPKEELTECKSSRNQLGMKRKTIKAFSQRKATRTIFVLAIVFTSLEGPVFIIQALTQSGMILTGNNNAKLRRQVFFLINLLVVFDSCCNVFVYMITSDKFRGEIRRLMG-VFGY----CTNGQQSFALGKL 349          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000016965.1 (SMESG000016965.1)

HSP 1 Score: 116.701 bits (291), Expect = 3.703e-29
Identity = 86/239 (35.98%), Postives = 143/239 (59.83%), Query Frame = 1
Query:  310 LTFWSINALLCSRNWMVMLVALARLEAIFRPLKIRSYSIFSSCKSRLYTXXXXXXXXXXXXXXXXYKKAIVCENENKIIFDSHWKNSSLYQCFEI-GYFIYQSVFPVVIVTISTLTMIYFLFKQT----FKSTHLLK-QYFRENE-------TQCMTSQQKATLTITVLAGVFTVTEIPIFII--TLLKFTYPNGLNNLMVLYQITRALVVVDSCCNFLIYVSTSEHYRRDLWYLFQRI 981
            + F++I +L C+RNW+V+++A +R E IF P   R    F+   +++  +I+L  S+ +S +R+F KKA++C++ N     +H  +   Y+ F+   +FIYQSV PVVIV I +L M + L   T    + STH+    YFR N        ++ + SQ+KAT TI VLA VFT+ E+P+FII   + +  + N     + L +++  LVV+DSC N ++Y+ TS+ +R ++  +F +I
Sbjct:   90 IVFYNITSLTCTRNWIVVIIACSRCEIIFVPFNSRRGLKFNHHNTKIIALIILFFSYCISFVRLFAKKALICQDTNVPQLANHLLDYRAYRLFKTYCFFIYQSVLPVVIVGIVSLAMSFSLLGSTRSSSYNSTHINDCSYFRMNHLSDQRHSSRKLNSQRKATRTILVLAVVFTLLEVPVFIIQSVVDRDDFSNHSILKIKLTELSNILVVLDSCSNIVVYLLTSDKFRLEVRRIFWQI 328          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000052331.1 (SMESG000052331.1)

HSP 1 Score: 80.4925 bits (197), Expect = 2.123e-16
Identity = 80/335 (23.88%), Postives = 156/335 (46.57%), Query Frame = 1
Query:  121 TNTLTVFTLSQPRFKGRSHNMRYTSFYHAIIELVLDLWIFTDKISMFAHLPDDKYVSAMIFSTLTFWSINALLCSRNWMVMLVALARLEAIFRPLKIRSYSIFSSCKSRLYTXXXXXXXXXXXXXXXXYKKAIVCENENKII-FDSHWKNSSLYQCFEI-GYFIYQSVFPVVIVTISTLTMIY----------------FLFK--------QTFKSTHLLKQYFRENETQCMTSQQKATLT-----------------------ITVLAGVFTVTEIPIFIITLLKFTYPNGLNNLMVLYQITRALVVVDSCCNFLIYVSTSEHYRRDLWYLFQR 978
             N++++   S+  F   +      S   ++ E+VL L + ++ I +   + +      M+ +++   S+NA+   RNW V+L+++AR E +  PL +R   IF+    R+ T +++++S  L+ + +   K I+C N + ++  D        Y  F+I G F++QS  PV+ + IST  + +                FL+K        +  KS+    + F +++ Q +    KAT                         +  L+ +F + +IP FIIT +   +PN L+  +     + +L+V+DS  NF +Y ++S+ +R  +  LF+R
Sbjct:   47 ANSISLIIFSKQYFDNSAVKFNLVSI--SVSEIVLQLVVVSEVIIVLGGVENVWKWREMLTTSVIHMSLNAMFSIRNWNVVLISVARCEVVISPLSLRGRLIFNKIVLRILTTLIVVISVVLACLNILKDKGIICVNLDWVVTIDGFLNQFDAYSYFDIYGIFMFQSCLPVIFICISTFIIGFKLRETPMKNKLLSKGPFLYKDCGKVSKPKIPKSSRFYNKGFFKSKFQLLQPNAKATSDENRKGKLISSISLIKNRIRTSYLVLFLSLMFCLLQIPTFIITCVLRFHPN-LDFEIHPDVFSNSLLVIDSFLNFFVYAASSKKFRYSVLRLFER 378          
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0R3UKE81.047e-2632.42G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A183SJQ71.828e-2430.43G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A3P7M9352.142e-2230.90G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A0R3T1S68.153e-2029.15G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A087VX962.926e-1827.50Rhodopsin orphan GPCR OS=Echinococcus multilocular... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO488021.524e-627.01Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000063918.10.000e+099.16SMESG000063918.1[more]
SMESG000078733.18.370e-3434.12SMESG000078733.1[more]
SMESG000031763.12.688e-3335.13SMESG000031763.1[more]
SMESG000016965.13.703e-2935.98SMESG000016965.1[more]
SMESG000052331.12.123e-1623.88SMESG000052331.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031189 ID=SMED30031189|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1165bp
AGAAACTGTTAAAATGTTTATTACTTTCAGAAAAATCTCAATTTTAGTTC
AAACGAATATGAGTTATTCGATTATTCGACGAATATTTTTATTGCGGTTG
TTGCGATTATTGGATTCGTCACGAATACTTTGACAGTTTTTACATTGTCC
CAGCCTCGATTTAAAGGCCGGTCTCACAACATGAGGTACACTTCGTTCTA
TCATGCGATTATTGAATTGGTGTTGGATTTGTGGATTTTTACCGACAAAA
TCTCCATGTTCGCTCATTTACCAGATGATAAATATGTATCAGCAATGATA
TTCAGCACTCTGACCTTTTGGTCTATTAATGCTTTATTGTGTTCAAGAAA
CTGGATGGTGATGCTGGTAGCATTGGCGAGATTGGAGGCGATTTTCAGAC
CATTAAAAATCCGATCTTACTCAATATTTTCCTCGTGTAAATCGAGATTA
TATACAATCATCGTTCTTTTGTTATCATTCTCTCTCTCCGTAATACGAGT
ATTCTACAAGAAAGCCATAGTTTGCGAGAATGAAAACAAAATAATTTTCG
ATTCTCATTGGAAAAACAGTTCTCTCTATCAATGTTTTGAAATTGGGTAT
TTTATTTATCAGTCTGTTTTTCCAGTCGTTATTGTGACGATTTCTACTTT
AACCATGATTTATTTCTTATTTAAACAAACATTCAAATCGACGCATTTAC
TCAAACAGTATTTCCGAGAGAATGAAACTCAATGTATGACATCTCAACAG
AAAGCCACTTTGACAATTACAGTTTTAGCCGGTGTTTTCACTGTGACAGA
AATCCCGATTTTTATAATCACTTTGTTAAAGTTCACATATCCCAACGGGT
TGAACAATCTCATGGTCCTCTATCAAATAACTCGAGCCCTCGTTGTCGTG
GATTCTTGTTGCAACTTTTTAATATATGTATCTACTAGTGAGCATTATCG
TCGAGATCTCTGGTATTTGTTTCAGCGGATATTTAGGTGGCATAAAATGT
TTGTCAACGAACAAGAAGAAAGTTTTGGTATGTCGAGAATTGTTAGTTAT
ATTCCAAATTCTAGGCGACACAAGGTTTAATCATTCAAATATCTTGTTTC
ATTATTTGCATAAACGATGAGAAAAATAATTTTATTTATGAAGATGTTAT
TTATTTAGTTTGTAG
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protein sequence of SMED30031189-orf-1

>SMED30031189-orf-1 ID=SMED30031189-orf-1|Name=SMED30031189-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=300bp
MRYTSFYHAIIELVLDLWIFTDKISMFAHLPDDKYVSAMIFSTLTFWSIN
ALLCSRNWMVMLVALARLEAIFRPLKIRSYSIFSSCKSRLYTIIVLLLSF
SLSVIRVFYKKAIVCENENKIIFDSHWKNSSLYQCFEIGYFIYQSVFPVV
IVTISTLTMIYFLFKQTFKSTHLLKQYFRENETQCMTSQQKATLTITVLA
GVFTVTEIPIFIITLLKFTYPNGLNNLMVLYQITRALVVVDSCCNFLIYV
STSEHYRRDLWYLFQRIFRWHKMFVNEQEESFGMSRIVSYIPNSRRHKV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway