SMED30031180

Overview
NameSMED30031180
Smed IDSMED30031180
Length (bp)3435
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30031180 (SMED30031180) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30031180 (SMED30031180) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30031180 (SMED30031180) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30031180 (SMED30031180) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30031180

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30031180SMESG000016699.1 dd_Smed_v4_10468_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30031180SMESG000016699.1 dd_Smed_v4_10468_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30031180 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001765:69766..77012 -15489
Homology
BLAST of SMED30031180 vs. TrEMBL
Match: A0A210QT33 (Uncharacterized protein OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT20716 PE=4 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 5.988e-6
Identity = 41/130 (31.54%), Postives = 68/130 (52.31%), Query Frame = 3
Query:  489 TLCPQINSSYP-KFSEPINTDVEMVDGKYESLNCCIFGVPKILWFFKPKYYNQPWKLKTQTLRVNGF-YILSDSNRS-LLFLQPKTYED--SGIYKCVGTNHSNNLISAEISLYVGN--GQKEFKIIGYS 857
            T CP+ N + P ++++ +N+   M+ G  +SL CCI G  +++W F+    N  W+       +NG  Y L ++NR+  L + PK   D   G Y+C  TN  N+ +   I LY+G   G++  +  G S
Sbjct:   54 TTCPESNETGPLRYTDGMNSADFMIAGHQKSLRCCITGFTRLIWSFRAT-ENDTWQ------HINGTDYQLDENNRNQTLMILPKQDSDPRPGSYRCTATNAENHTLIHRIELYLGGPTGREHPQAFGPS 176          
BLAST of SMED30031180 vs. Planmine SMEST
Match: SMESG000016699.1 (SMESG000016699.1)

HSP 1 Score: 333.954 bits (855), Expect = 5.153e-106
Identity = 174/185 (94.05%), Postives = 178/185 (96.22%), Query Frame = 3
Query:  555 MVDGKYESLNCCIFGVPKILWFFKPKYYNQPWKLKTQTLRVNGFYILSDSNRSLLFLQPKTYEDSGIYKCVGTNHSNNLISAEISLYVGNGQKEFKIIGYSDPTKMYRVNSEHTIFCSVKAHKKSFCIFTWNRVIENQNRSIIASSXXXXXXXXXXXXXGYSGYASLSWMSVNRLEYLNNFKTNI 1109
            MVDGKYESLNCCIFGVPKILWFFKPKYYNQPWKLKTQTLRVNGFYILSDSNRSLLFLQPKTYEDSGIYKCVGTNHSNNLISAEISLYVGNGQKEFKIIGYSDPTKMYRVNSEHTIFCSVKAHKKSFCIFTWNRVIENQNRSIIASSNLTTDRTNDTLTNGYSGYASLSWMS+ +   + NF  N+
Sbjct:    1 MVDGKYESLNCCIFGVPKILWFFKPKYYNQPWKLKTQTLRVNGFYILSDSNRSLLFLQPKTYEDSGIYKCVGTNHSNNLISAEISLYVGNGQKEFKIIGYSDPTKMYRVNSEHTIFCSVKAHKKSFCIFTWNRVIENQNRSIIASSNLTTDRTNDTLTNGYSGYASLSWMSLEKNHRIYNFTLNL 185          

HSP 2 Score: 114.005 bits (284), Expect = 1.039e-27
Identity = 72/74 (97.30%), Postives = 73/74 (98.65%), Query Frame = 1
Query: 1918 FRSLEKNHRIYNFTLNLKSLNESQEGYYSCHITESSANSEXXXXXXXXXXXXNMIFLVIELIVSFIVILAILRA 2139
            + SLEKNHRIYNFTLNLKSLNESQEGYYSCHITESSANSELLHLFHFRLYRRNMIFLVIELIVSFIVILAILRA
Sbjct:  169 WMSLEKNHRIYNFTLNLKSLNESQEGYYSCHITESSANSELLHLFHFRLYRRNMIFLVIELIVSFIVILAILRA 242          
BLAST of SMED30031180 vs. Planmine SMEST
Match: SMESG000016699.1 (SMESG000016699.1)

HSP 1 Score: 333.569 bits (854), Expect = 1.751e-103
Identity = 174/185 (94.05%), Postives = 178/185 (96.22%), Query Frame = 3
Query:  555 MVDGKYESLNCCIFGVPKILWFFKPKYYNQPWKLKTQTLRVNGFYILSDSNRSLLFLQPKTYEDSGIYKCVGTNHSNNLISAEISLYVGNGQKEFKIIGYSDPTKMYRVNSEHTIFCSVKAHKKSFCIFTWNRVIENQNRSIIASSXXXXXXXXXXXXXGYSGYASLSWMSVNRLEYLNNFKTNI 1109
            MVDGKYESLNCCIFGVPKILWFFKPKYYNQPWKLKTQTLRVNGFYILSDSNRSLLFLQPKTYEDSGIYKCVGTNHSNNLISAEISLYVGNGQKEFKIIGYSDPTKMYRVNSEHTIFCSVKAHKKSFCIFTWNRVIENQNRSIIASSNLTTDRTNDTLTNGYSGYASLSWMS+ +   + NF  N+
Sbjct:    1 MVDGKYESLNCCIFGVPKILWFFKPKYYNQPWKLKTQTLRVNGFYILSDSNRSLLFLQPKTYEDSGIYKCVGTNHSNNLISAEISLYVGNGQKEFKIIGYSDPTKMYRVNSEHTIFCSVKAHKKSFCIFTWNRVIENQNRSIIASSNLTTDRTNDTLTNGYSGYASLSWMSLEKNHRIYNFTLNL 185          

HSP 2 Score: 113.62 bits (283), Expect = 3.308e-26
Identity = 72/74 (97.30%), Postives = 73/74 (98.65%), Query Frame = 1
Query: 1918 FRSLEKNHRIYNFTLNLKSLNESQEGYYSCHITESSANSEXXXXXXXXXXXXNMIFLVIELIVSFIVILAILRA 2139
            + SLEKNHRIYNFTLNLKSLNESQEGYYSCHITESSANSELLHLFHFRLYRRNMIFLVIELIVSFIVILAILRA
Sbjct:  169 WMSLEKNHRIYNFTLNLKSLNESQEGYYSCHITESSANSELLHLFHFRLYRRNMIFLVIELIVSFIVILAILRA 242          
BLAST of SMED30031180 vs. Planmine SMEST
Match: SMESG000016701.1 (SMESG000016701.1)

HSP 1 Score: 83.9593 bits (206), Expect = 1.894e-16
Identity = 78/216 (36.11%), Postives = 109/216 (50.46%), Query Frame = 3
Query:  435 ILLIRIIVFP-KFGSSGYFTLCP-----QINSSYPKFSEPINTDVEMVDGKYESLNCCIFGVPKILWFFKPKYYNQPWKLKTQTLRVNGFYILSDSNRSLLFLQPKTYEDSGIYKCVGTNHSNNLISAEISLYVGNG--QKEFKIIGYSDPTKMYRVN-SEHTIFCSVKAHKKSFCIFTWNRVIENQNRSIIASSXXXXXXXXXXXXXGYSGYASL 1055
            IL+I ++V      S G    CP     Q+N + P F+  +     M       ++CCI+GV  I W+FK    N   K  TQ ++    Y L ++NR L+ L   +    GIYKCVG N   N +S +  + V      K+ +I+  SD T++  V  S +T +C VK    S C F+WNR    +  SI+A SNLT  RT DTL  GYSGY+ L
Sbjct:    9 ILIINVLVLTLGLESDGK---CPEPRSEQLNQNQPFFTRDMYESSVMSVSMIGYVHCCIYGVSNINWWFK----NSDSKHFTQIIKGKD-YQLENNNRKLIVLNV-SKNSKGIYKCVGNNEKGNSLSNQTFVIVVQSTYNKDIEIVDASDITELTFVPYSNYTFYCLVKVFPGSICNFSWNRKKNGKRTSILAHSNLTKKRTYDTLVEGYSGYSQL 215          
BLAST of SMED30031180 vs. Planmine SMEST
Match: SMESG000016701.1 (SMESG000016701.1)

HSP 1 Score: 83.9593 bits (206), Expect = 2.270e-16
Identity = 78/216 (36.11%), Postives = 109/216 (50.46%), Query Frame = 3
Query:  435 ILLIRIIVFP-KFGSSGYFTLCP-----QINSSYPKFSEPINTDVEMVDGKYESLNCCIFGVPKILWFFKPKYYNQPWKLKTQTLRVNGFYILSDSNRSLLFLQPKTYEDSGIYKCVGTNHSNNLISAEISLYVGNG--QKEFKIIGYSDPTKMYRVN-SEHTIFCSVKAHKKSFCIFTWNRVIENQNRSIIASSXXXXXXXXXXXXXGYSGYASL 1055
            IL+I ++V      S G    CP     Q+N + P F+  +     M       ++CCI+GV  I W+FK    N   K  TQ ++    Y L ++NR L+ L   +    GIYKCVG N   N +S +  + V      K+ +I+  SD T++  V  S +T +C VK    S C F+WNR    +  SI+A SNLT  RT DTL  GYSGY+ L
Sbjct:   31 ILIINVLVLTLGLESDGK---CPEPRSEQLNQNQPFFTRDMYESSVMSVSMIGYVHCCIYGVSNINWWFK----NSDSKHFTQIIKGKD-YQLENNNRKLIVLNV-SKNSKGIYKCVGNNEKGNSLSNQTFVIVVQSTYNKDIEIVDASDITELTFVPYSNYTFYCLVKVFPGSICNFSWNRKKNGKRTSILAHSNLTKKRTYDTLVEGYSGYSQL 237          
BLAST of SMED30031180 vs. Planmine SMEST
Match: SMESG000016701.1 (SMESG000016701.1)

HSP 1 Score: 83.1889 bits (204), Expect = 3.818e-16
Identity = 72/195 (36.92%), Postives = 100/195 (51.28%), Query Frame = 3
Query:  495 CP-----QINSSYPKFSEPINTDVEMVDGKYESLNCCIFGVPKILWFFKPKYYNQPWKLKTQTLRVNGFYILSDSNRSLLFLQPKTYEDSGIYKCVGTNHSNNLISAEISLYVGNG--QKEFKIIGYSDPTKMYRVN-SEHTIFCSVKAHKKSFCIFTWNRVIENQNRSIIASSXXXXXXXXXXXXXGYSGYASL 1055
            CP     Q+N + P F+  +     M       ++CCI+GV  I W+FK    N   K  TQ ++    Y L ++NR L+ L   +    GIYKCVG N   N +S +  + V      K+ +I+  SD T++  V  S +T +C VK    S C F+WNR    +  SI+A SNLT  RT DTL  GYSGY+ L
Sbjct:   31 CPEPRSEQLNQNQPFFTRDMYESSVMSVSMIGYVHCCIYGVSNINWWFK----NSDSKHFTQIIKGKD-YQLENNNRKLIVLNV-SKNSKGIYKCVGNNEKGNSLSNQTFVIVVQSTYNKDIEIVDASDITELTFVPYSNYTFYCLVKVFPGSICNFSWNRKKNGKRTSILAHSNLTKKRTYDTLVEGYSGYSQL 219          
The following BLAST results are available for this feature:
BLAST of SMED30031180 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031180 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031180 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031180 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031180 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031180 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031180 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031180 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
A0A210QT335.988e-631.54Uncharacterized protein OS=Mizuhopecten yessoensis... [more]
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BLAST of SMED30031180 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031180 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031180 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031180 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031180 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031180 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000016699.15.153e-10694.05SMESG000016699.1[more]
SMESG000016699.11.751e-10394.05SMESG000016699.1[more]
SMESG000016701.11.894e-1636.11SMESG000016701.1[more]
SMESG000016701.12.270e-1636.11SMESG000016701.1[more]
SMESG000016701.13.818e-1636.92SMESG000016701.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031180 ID=SMED30031180|Name=SMED30031180|organism=Schmidtea mediterranea sexual|type=transcript|length=3435bp
CGATCTATTTTACTGCTGATGATTGATTTTTAGTGGAATATTATGCTTTC
AATGACTTAGAGTTGGACTTCTTTCAATTAGGATATTTTACTGCTGATGA
TTGATTGGATGTTTGGATTGCGCGCTCTTCTTGTATGTGTGTGTGTGTGT
GTTGGTGTGGTTGGCATAGAATTTGTTATATCTGTAAATCATAGTTGATT
TTATTATGTGATAGATAGTTGTTGAAGTAAATAAAACTGAAGATGAAATG
ATAATAATAATAATTTTAATATTATTATTATTATTATCATAATTATCATT
ATTGGCTTTTTCCTTATCCAATTGAAAATGTGATTCATTTTACTGATTGC
GAAGTCAAAGTTCAAATATGTTTTCTGATAAACAAATTAACAAAGGATTT
CATGATGTATTTTTTCGAATGCTTTAAGCTTGCTATCCTCCTCATACGAA
TAATAGTATTCCCGAAATTCGGTTCAAGTGGATATTTTACTTTGTGTCCC
CAAATAAACTCATCTTATCCAAAGTTTTCAGAACCGATAAATACGGACGT
AGAAATGGTTGATGGGAAATACGAGTCATTAAACTGCTGTATTTTCGGAG
TGCCAAAAATTTTGTGGTTTTTCAAACCCAAATATTATAATCAGCCATGG
AAATTAAAAACTCAAACCCTAAGGGTGAATGGATTTTATATCCTCTCTGA
TTCCAATAGGTCTCTATTGTTTTTGCAACCGAAGACCTACGAAGACAGTG
GAATATACAAATGTGTCGGGACGAATCATTCAAATAATTTAATAAGTGCA
GAAATAAGTTTGTATGTGGGAAATGGTCAGAAAGAATTTAAAATAATTGG
ATATAGTGATCCGACCAAAATGTATAGAGTCAATTCAGAGCATACAATAT
TCTGTTCAGTGAAAGCCCATAAAAAATCATTTTGCATTTTTACATGGAAT
AGAGTAATAGAAAATCAAAATCGGTCTATCATTGCAAGCTCCAATTTAAC
AACGGACAGGACCAATGATACCCTTACCAATGGCTATTCTGGATACGCAA
GTTTGAGCTGGATGTCAGTAAATCGGCTTGAATATTTAAATAATTTCAAA
ACAAACATATCAGTTTAATTATGTATAAAAAATATATTTCATAATTTTCA
GAAGCCTTTCTTAACTTTGTGGCTAATAATTAAAGTGTGGTTGACATAAA
ATTTCATAAAAACCAAATTAACAGGATATACGATCCGAAGAAATTGTATT
GACAATGAAAGTGGATTATCAAACGTTAACTGTTGCATATGTGCAACAAT
GAGTCTGATGTACTTAGAAACACAAAATTCATATGAAAAATTTTTTAACT
TCATATTTTAATGTTGTAAATGTACTTGCAACACAGAGCTAAATGTACGA
AGTTTCAATGTGATTTGATATGCCAACTAAATACTGGAAGAATTCAATAG
TGCTAATGTAATTGATTAGACGGCAACTTTAACAGAAAAATGTGAAAAAT
CTAATTTCCATTTTTTGATTATCAAATTTGGTTTTAGCAGGTGAAAATTA
TGGAACAATTGTATTTGGTGTTAAATATATGTTGCAAGCATCAGATTTTA
AAAAAACTATATAATTTAAAATTTATGACCTACTTTATTTTGCCTTAAAA
CTATATAATCTGACATTAAGATAACATTAAATTGACCTTAAACCGACATA
GGTCTACTTATTTGATCTTAAGTTGACCTACTTTCAAAAAAATACTTTTT
TTAGCTTAATATGTTTATTCTAGGAGTTGTTGGCCTATATGTGACGAGCT
TACAGAACATATCTATAAAAATTAGTACTCCGTTGGGTATTTTATACATT
TAATTGTGTTTTTAATAATCCTATTCATAAAATATTATGCAATAGTTTGT
TTAATTAGGTTTTCTATTTTAGGTCATTAGAAAAGAATCATCGGATTTAT
AATTTTACTTTGAATTTGAAGAGTCTTAACGAATCTCAGGAAGGTTATTA
CAGTTGTCATATTACTGAAAGTTCAGCGAATTCGGAACTTCTGCATTTAT
TTCATTTCCGATTGTACAGAAGAAATATGATATTTCTGGTTATTGAATTA
ATTGTCAGTTTCATTGTTATATTGGCTATTTTGCGCGCATTCCAATATGA
TATTAAGATTATTATCTTTCAAATTATTCGCAAGTTTATTAAGCAAAGTA
AGACATTTTAATTAATATAAATTGCGTGTTTAGCTATAACAATGGAATAT
GGAAGACCTCAAAGTCCAATCAACCTTTCTTCCAAATTCTGTGTGATATG
GATAAGATAAGCTCTTAAACTCGTTATGGTTAGGTTTAAGGGTTATGTTT
GGGAGGTTAAGGGTCAGTCCTAAGGGGTTAGGAGTAAATCTATTGATATA
TTTTACTAATGGTTGCTAATAAACATGGCAGCAAATTCGAATTTGCAGCA
TTATCCGTATTTTTTGTTTTTGAGGAAGACGAAAGATAAAGTCATCATTT
ATAAACTGCACGAAGCATGTAGAAATAAATCACATATAATTGGATAAGAG
AGTTTTAAACCGGACAGGAAAGTTGAAGCTTATTTCGAAAGAATAGTAAT
TAATTACTTACAATGAACTATTAACTCGACTTAATAAATAAACATGAAGA
CTGTGTATAACAGCCTGGAACGCATATGAACAAGAAAATTTCAGTCGATT
TGCTCCACGTTTCTTAAAAATAGAAATGCGAGAAAAGCGAATTCATACAT
GCTTAGAAAATCTGATTCTTTTTCATGCTCTTGGAATGAAAGAATGTTTT
GAAAAATATAATACGGGAGGACGAGAAGAATTTCAATATTCCAAAAATAT
TTCGTGAAAAAATGTCTCGTGTTCAGTGTGTTTTGGGATGCAAAAAAGGT
TTGATCAAGATCAACTTTATTCCGTAAATTATCGACTTTCACTACAACTG
TGCACGAGTTAGTGATTCTCGAAAGTTACAACTGATTCGCATGAATCAAG
GTATCTATATGTTGCATGAAAATTCACCAGTATATACAGCTCATCTAACT
GCGTGTACAATCCCCAAAAATGGTTTTACACTAGTTTCATATCCATCGTA
CAGCTCTGATTTGAGTTTTCTTGCTAAATCACCTAAAGAAATATTTGCGG
GAATTCAGTTTAAATATTATCTAACAAGACAAGTACAAGATTTCCTAAAT
GGAAAGGAAGTCACTTTCGTCGAAAATAGTATTTTTTCATTGGTAAATTG
TATTGAAGCAGAAGATGATTATATAAAAAATAAATGTGTGCTTTTTACCT
ATGCAGTTCACTTATTGTAAATATAAAGTCTCATTAGAGCAGGGGTGAGC
AACCTTTGGCGGGCCACAAAACAAATTTTCGCAGGCCGCAGGTTGCTCAT
CCCTGCATTAGAGTACCATTTATATTTATGTATGA
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protein sequence of SMED30031180-orf-1

>SMED30031180-orf-1 ID=SMED30031180-orf-1|Name=SMED30031180-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=239bp
MMYFFECFKLAILLIRIIVFPKFGSSGYFTLCPQINSSYPKFSEPINTDV
EMVDGKYESLNCCIFGVPKILWFFKPKYYNQPWKLKTQTLRVNGFYILSD
SNRSLLFLQPKTYEDSGIYKCVGTNHSNNLISAEISLYVGNGQKEFKIIG
YSDPTKMYRVNSEHTIFCSVKAHKKSFCIFTWNRVIENQNRSIIASSNLT
TDRTNDTLTNGYSGYASLSWMSVNRLEYLNNFKTNISV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR036179Ig-like_dom_sf
IPR013783Ig-like_fold
IPR013098Ig_I-set
IPR007110Ig-like_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013098Immunoglobulin I-setPFAMPF07679I-setcoord: 40..128
e-value: 2.3E-5
score: 24.4
IPR013783Immunoglobulin-like foldGENE3DG3DSA:2.60.40.10coord: 34..148
e-value: 4.7E-7
score: 31.7
IPR007110Immunoglobulin-like domainPROSITEPS50835IG_LIKEcoord: 40..136
score: 9.627
IPR036179Immunoglobulin-like domain superfamilySUPERFAMILYSSF48726Immunoglobulincoord: 40..139