FMRFamide-activated amiloride-sensitive sodium channel

Overview
NameFMRFamide-activated amiloride-sensitive sodium channel
Smed IDSMED30031176
Length (bp)1764
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of FMRFamide-activated amiloride-sensitive sodium channel (SMED30031176) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for FMRFamide-activated amiloride-sensitive sodium channel (SMED30031176) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30031176

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
muscle cellSMED30031176h1SMcG0020023 dd_Smed_v4_14040_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30031176h1SMcG0020023 dd_Smed_v4_14040_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
dorsal-ventral muscle cellSMED30031176h1SMcG0020023 dd_Smed_v4_14040_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Human
Match: SCNN1A (sodium channel epithelial 1 alpha subunit [Source:HGNC Symbol;Acc:HGNC:10599])

HSP 1 Score: 61.2326 bits (147), Expect = 4.291e-9
Identity = 81/350 (23.14%), Postives = 129/350 (36.86%), Query Frame = 2
Query:  614 QDSISQLKISLFSHQDFFNCYTIEGEQE---YTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQP-----NLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIY 1639
            Q S +Q   S F H  + NCYT   +     +  S+ G+   L ++   E     ++  I +L+   G ++++H       +D+ GFNL P    SI   +    RL     +C  N       NLY      KYT + C+ + FQ  +I +C C    +PRP     +  YC   +Y  +S        C  K+Q  FS +      + C   C  ++Y+ +   ++W +   +    Q L R                                         N   V NK N +  V+I  K        E   +   TLLS +G   SL  G +   V E+ E ++
Sbjct:  286 QVSCNQANYSHFHHPMYGNCYTFNDKNNSNLWMSSMPGINNGLSLMLRAE-----QNDFIPLLSTVTGARVMVHGQDEPAFMDDGGFNLRPGVETSISMRKETLDRLGGDYGDCTKNGSDVPVENLYPS----KYTQQVCIHSCFQESMIKECGCAYIFYPRP----QNVEYC---DYRKHSSW----GYCYYKLQVDFSSDHLGCFTK-CRKPCSVTSYQLSAGYSRWPSVTSQEWVFQMLSRQ----------------------------------------NNYTVNNKRNGVAKVNIFFKELNYKTNSESPSVTMVTLLSNLGSQWSLWFGSSVLSVVEMAELVF 574          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Human
Match: SCNN1A (sodium channel epithelial 1 alpha subunit [Source:HGNC Symbol;Acc:HGNC:10599])

HSP 1 Score: 60.8474 bits (146), Expect = 5.515e-9
Identity = 81/350 (23.14%), Postives = 129/350 (36.86%), Query Frame = 2
Query:  614 QDSISQLKISLFSHQDFFNCYTIEGEQE---YTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQP-----NLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIY 1639
            Q S +Q   S F H  + NCYT   +     +  S+ G+   L ++   E     ++  I +L+   G ++++H       +D+ GFNL P    SI   +    RL     +C  N       NLY      KYT + C+ + FQ  +I +C C    +PRP     +  YC   +Y  +S        C  K+Q  FS +      + C   C  ++Y+ +   ++W +   +    Q L R                                         N   V NK N +  V+I  K        E   +   TLLS +G   SL  G +   V E+ E ++
Sbjct:  309 QVSCNQANYSHFHHPMYGNCYTFNDKNNSNLWMSSMPGINNGLSLMLRAE-----QNDFIPLLSTVTGARVMVHGQDEPAFMDDGGFNLRPGVETSISMRKETLDRLGGDYGDCTKNGSDVPVENLYPS----KYTQQVCIHSCFQESMIKECGCAYIFYPRP----QNVEYC---DYRKHSSW----GYCYYKLQVDFSSDHLGCFTK-CRKPCSVTSYQLSAGYSRWPSVTSQEWVFQMLSRQ----------------------------------------NNYTVNNKRNGVAKVNIFFKELNYKTNSESPSVTMVTLLSNLGSQWSLWFGSSVLSVVEMAELVF 597          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Human
Match: SCNN1A (sodium channel epithelial 1 alpha subunit [Source:HGNC Symbol;Acc:HGNC:10599])

HSP 1 Score: 60.077 bits (144), Expect = 8.481e-9
Identity = 81/350 (23.14%), Postives = 129/350 (36.86%), Query Frame = 2
Query:  614 QDSISQLKISLFSHQDFFNCYTIEGEQE---YTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQP-----NLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIY 1639
            Q S +Q   S F H  + NCYT   +     +  S+ G+   L ++   E     ++  I +L+   G ++++H       +D+ GFNL P    SI   +    RL     +C  N       NLY      KYT + C+ + FQ  +I +C C    +PRP     +  YC   +Y  +S        C  K+Q  FS +      + C   C  ++Y+ +   ++W +   +    Q L R                                         N   V NK N +  V+I  K        E   +   TLLS +G   SL  G +   V E+ E ++
Sbjct:  345 QVSCNQANYSHFHHPMYGNCYTFNDKNNSNLWMSSMPGINNGLSLMLRAE-----QNDFIPLLSTVTGARVMVHGQDEPAFMDDGGFNLRPGVETSISMRKETLDRLGGDYGDCTKNGSDVPVENLY----PSKYTQQVCIHSCFQESMIKECGCAYIFYPRP----QNVEYC---DYRKHSSW----GYCYYKLQVDFSSDHLGCFTK-CRKPCSVTSYQLSAGYSRWPSVTSQEWVFQMLSRQ----------------------------------------NNYTVNNKRNGVAKVNIFFKELNYKTNSESPSVTMVTLLSNLGSQWSLWFGSSVLSVVEMAELVF 633          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Human
Match: SCNN1G (sodium channel epithelial 1 gamma subunit [Source:HGNC Symbol;Acc:HGNC:10602])

HSP 1 Score: 58.9214 bits (141), Expect = 1.866e-8
Identity = 55/225 (24.44%), Postives = 95/225 (42.22%), Query Frame = 2
Query:  641 SLFSHQDFFNCYTI---EGEQEYTKSIVGLIFILDIVPSDEIY---ELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQP-----NLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKA 1282
            +LF H    NCYT    E E   + S+ G  + L ++    +Y   E    FL+       G K++IH     P +++ G  +  + + SI    T+ ++L  P   C ++       N+Y+      Y+ + C+ + FQT+++ KC C   + P P   N    YC   NY  +   +     C  ++   F +  E+     C   C +  +  T SL QW +
Sbjct:  273 TLFHHPMHGNCYTFNNRENETILSTSMGGSEYGLQVI----LYINEEEYNPFLVS----STGAKVIIHRQDEYPFVEDVGTEIETAMVTSIGMHLTESFKLSEPYSQCTEDGSDVPIRNIYNAA----YSLQICLHSCFQTKMVEKCGCAQYSQPLPPAAN----YC---NYQQHPNWM----YCYYQLHRAF-VQEELGCQSVCKEACSFKEWTLTTSLAQWPS 473          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Human
Match: SCNN1D (sodium channel epithelial 1 delta subunit [Source:HGNC Symbol;Acc:HGNC:10601])

HSP 1 Score: 56.6102 bits (135), Expect = 9.464e-8
Identity = 50/250 (20.00%), Postives = 106/250 (42.40%), Query Frame = 2
Query:  647 FSHQDFFNCYTIEG----EQEYTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQPNL-YDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQW---KASEQKLEHLQKLYRSYFSLKQKQSKENINNV 1372
            F H  + +CYT++G    ++      VGL+  ++  P            + +L+   G+++++H  ++ P + +  F++ P    +I   + + +RL +P  +C      +  +L  +  YT + C+ + FQ  ++  C C    HP P    +   YC    +            C  ++ +    +R    ++ C   CR S ++ +   ++W   K++   L  L +    + S +Q+ S   IN V
Sbjct:  278 FHHPTYGSCYTVDGVWTAQRPGITHGVGLVLRVEQQPH-----------LPLLSTLAGIRVMVHGRNHTPFLGHHSFSVRPGTEATISIREDEVHRLGSPYGHCTAGGEGVEVELLHNTSYTRQACLVSCFQQLMVETCSCGYYLHPLP----AGAEYCSSARHPAWGH-------CFYRLYQDLETHRLPCTSR-CPRPCRESAFKLSTGTSRWPSAKSAGWTLATLGEQGLPHQSHRQRSSLAKINIV 504          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Celegans
Match: asic-2 (Degenerin-like protein asic-2 [Source:UniProtKB/Swiss-Prot;Acc:Q22851])

HSP 1 Score: 55.0694 bits (131), Expect = 1.146e-7
Identity = 40/164 (24.39%), Postives = 74/164 (45.12%), Query Frame = 2
Query:  806 GMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQPNLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNS-EILDKRFSCVEKV---QEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKAS 1285
            G +I ++   + P +D++G +      +++  S T   R + P   C D +P+  D  + F YT + C     Q + I KCQC    +PR           L L  T+ + + +     C++ +   Q   + N +++    C+  C  STY  T+SL Q+ ++
Sbjct:  238 GARIGVNSRGSSPGLDSNGIDAGVGYESAVSVSLTQNVRAKKPYGTCVDREPDSSDYYKDFIYTLETCFNGCKQRDTIAKCQCA---NPR-----------LALGSTDTACQPIKADLDCLQTLKGNQTSSTPNIDLLVECNCNPPCDESTYTPTVSLAQFPST 387          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Celegans
Match: unc-105 (Degenerin-like protein unc-105 [Source:UniProtKB/Swiss-Prot;Acc:Q09274])

HSP 1 Score: 53.1434 bits (126), Expect = 4.787e-7
Identity = 59/283 (20.85%), Postives = 112/283 (39.58%), Query Frame = 2
Query:  806 GMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNC-HDNQPNLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEK----VQEHFSLNRE-IIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFI 1636
            G +I +H+ H +P  D  G++     M+S       + RL  P  +C H  +     +   F+Y+ + C  +  Q  ++  C C    +P   +  ++   C  +N       +D+R  C+      + E +S  +E II + +C   C+ + YE T S  +W +   K+  ++ L   +  L                           E  RK    N ++V+    +L Y ++          +E       ++L+ +GGL+ L IG +   + EI+  I
Sbjct:  427 GFRITVHDKHIVPFPDAFGYSAPTGFMSSFGVRMKQFIRLEPPYGHCRHGGEDAATFVYTGFQYSVEACHRSCAQKVIVEACGCADPMYPVAEMFGNNTKPCQAVN-------MDQR-ECLRNTTLWLGELYSKGKEAIIPDCYCHQPCQETNYEVTYSSARWPSGSAKV--MECLPGDFLCL---------------------------EKYRK----NAAMVQIFYEELNYETM----------QESPAYTLTSVLADLGGLTGLWIGASVVSLLEIVTLI 658          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Celegans
Match: unc-105 (Degenerin-like protein unc-105 [Source:UniProtKB/Swiss-Prot;Acc:Q09274])

HSP 1 Score: 53.1434 bits (126), Expect = 4.787e-7
Identity = 59/283 (20.85%), Postives = 112/283 (39.58%), Query Frame = 2
Query:  806 GMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNC-HDNQPNLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEK----VQEHFSLNRE-IIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFI 1636
            G +I +H+ H +P  D  G++     M+S       + RL  P  +C H  +     +   F+Y+ + C  +  Q  ++  C C    +P   +  ++   C  +N       +D+R  C+      + E +S  +E II + +C   C+ + YE T S  +W +   K+  ++ L   +  L                           E  RK    N ++V+    +L Y ++          +E       ++L+ +GGL+ L IG +   + EI+  I
Sbjct:  427 GFRITVHDKHIVPFPDAFGYSAPTGFMSSFGVRMKQFIRLEPPYGHCRHGGEDAATFVYTGFQYSVEACHRSCAQKVIVEACGCADPMYPVAEMFGNNTKPCQAVN-------MDQR-ECLRNTTLWLGELYSKGKEAIIPDCYCHQPCQETNYEVTYSSARWPSGSAKV--MECLPGDFLCL---------------------------EKYRK----NAAMVQIFYEELNYETM----------QESPAYTLTSVLADLGGLTGLWIGASVVSLLEIVTLI 658          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Celegans
Match: unc-105 (Degenerin-like protein unc-105 [Source:UniProtKB/Swiss-Prot;Acc:Q09274])

HSP 1 Score: 53.1434 bits (126), Expect = 5.121e-7
Identity = 59/283 (20.85%), Postives = 112/283 (39.58%), Query Frame = 2
Query:  806 GMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNC-HDNQPNLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEK----VQEHFSLNRE-IIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFI 1636
            G +I +H+ H +P  D  G++     M+S       + RL  P  +C H  +     +   F+Y+ + C  +  Q  ++  C C    +P   +  ++   C  +N       +D+R  C+      + E +S  +E II + +C   C+ + YE T S  +W +   K+  ++ L   +  L                           E  RK    N ++V+    +L Y ++          +E       ++L+ +GGL+ L IG +   + EI+  I
Sbjct:  447 GFRITVHDKHIVPFPDAFGYSAPTGFMSSFGVRMKQFIRLEPPYGHCRHGGEDAATFVYTGFQYSVEACHRSCAQKVIVEACGCADPMYPVAEMFGNNTKPCQAVN-------MDQR-ECLRNTTLWLGELYSKGKEAIIPDCYCHQPCQETNYEVTYSSARWPSGSAKV--MECLPGDFLCL---------------------------EKYRK----NAAMVQIFYEELNYETM----------QESPAYTLTSVLADLGGLTGLWIGASVVSLLEIVTLI 678          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Celegans
Match: unc-105 (Degenerin-like protein unc-105 [Source:UniProtKB/Swiss-Prot;Acc:Q09274])

HSP 1 Score: 53.1434 bits (126), Expect = 5.121e-7
Identity = 59/283 (20.85%), Postives = 112/283 (39.58%), Query Frame = 2
Query:  806 GMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNC-HDNQPNLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEK----VQEHFSLNRE-IIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFI 1636
            G +I +H+ H +P  D  G++     M+S       + RL  P  +C H  +     +   F+Y+ + C  +  Q  ++  C C    +P   +  ++   C  +N       +D+R  C+      + E +S  +E II + +C   C+ + YE T S  +W +   K+  ++ L   +  L                           E  RK    N ++V+    +L Y ++          +E       ++L+ +GGL+ L IG +   + EI+  I
Sbjct:  447 GFRITVHDKHIVPFPDAFGYSAPTGFMSSFGVRMKQFIRLEPPYGHCRHGGEDAATFVYTGFQYSVEACHRSCAQKVIVEACGCADPMYPVAEMFGNNTKPCQAVN-------MDQR-ECLRNTTLWLGELYSKGKEAIIPDCYCHQPCQETNYEVTYSSARWPSGSAKV--MECLPGDFLCL---------------------------EKYRK----NAAMVQIFYEELNYETM----------QESPAYTLTSVLADLGGLTGLWIGASVVSLLEIVTLI 678          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Xenopus
Match: copg2 (coatomer protein complex subunit gamma 2 [Source:Xenbase;Acc:XB-GENE-941264])

HSP 1 Score: 75.485 bits (184), Expect = 1.242e-13
Identity = 79/337 (23.44%), Postives = 130/337 (38.58%), Query Frame = 2
Query:  647 FSHQDFFNCYTIEG---EQEYTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQPNLYDLGRSF----KYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFI 1636
            F H  + NC+TI     E  +  S  G  + L IV   ++++      + +L++  G +I+IH P+  P +++ GF++ P   NSI   Q +  RL      C  +     DLG        YT + C+ + FQ ++I  C C    +P P        YC   NY  + +     +   EK+ +H      +I    C  +C+ + Y  +    +W +   K + L  L   Y S  +                        R ++ K +   + L    + +   VS+T                   LLS MGGL S   G +   VAEI E +
Sbjct:  256 FHHPIYGNCFTINNHWKENSWYSSRPGKQYGLSIVVKTDLHDN-----MPLLSQAAGARIMIHNPNQPPLVEHQGFDIRPGTENSISVKQEEAIRLGGKYSQCTSDGS---DLGIKILYNTSYTMQACLNSCFQYKMIEVCGCGYYFYPLP----PGMEYC---NYNKHPDWGYCFYQLYEKMLDH-----TLICFTQCPKQCKQTEYHLSAGTAKWPSPVSKAKKLLSLQERYNSTSK------------------------RSDISKINVYYEELSYRSVEETPSVSVT------------------MLLSSMGGLWSWWFGSSVLSVAEIAELV 530          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Xenopus
Match: copg2 (coatomer protein complex subunit gamma 2 [Source:Xenbase;Acc:XB-GENE-941264])

HSP 1 Score: 70.8626 bits (172), Expect = 2.971e-12
Identity = 78/337 (23.15%), Postives = 133/337 (39.47%), Query Frame = 2
Query:  647 FSHQDFFNCYTIEG---EQEYTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQPNLYDLGRSF----KYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFI 1636
            F H  + NC+TI     E  +  S  G  + L IV   ++++      + +L++  G +I+IH P+  P +++ GF++ P   NSI   Q +  RL      C  +     DLG        YT + C+ + FQ ++I  C C    +P P        YC   NY  + +     +   EK+ +H      +I    C  +C+ + Y  +    +W +       + K+   Y ++K K            +   + +  D    + K +   + L    + +   VS+T                   LLS MGGL S   G +   VAEI E +
Sbjct:  256 FHHPIYGNCFTINNHWKENSWYSSRPGKQYGLSIVVKTDLHDN-----MPLLSQAAGARIMIHNPNQPPLVEHQGFDIRPGTENSISVKQEEAIRLGGKYSQCTSDGS---DLGIKILYNTSYTMQACLNSCFQYKMIEVCGCGYYFYPLP----PGMEYC---NYNKHPDWGYCFYQLYEKMLDH-----TLICFTQCPKQCKQTEYHLSAGTAKWPSP------VSKVSNDYITIKAK------------VYWGVWELSD----ISKINVYYEELSYRSVEETPSVSVT------------------MLLSSMGGLWSWWFGSSVLSVAEIAELV 532          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Xenopus
Match: cdh12 (cadherin 12, type 2 (N-cadherin 2) [Source:Xenbase;Acc:XB-GENE-982876])

HSP 1 Score: 62.7734 bits (151), Expect = 1.088e-9
Identity = 84/341 (24.63%), Postives = 134/341 (39.30%), Query Frame = 2
Query:  641 SLFSHQDFFNCYTIEGEQE---YTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQP-----NLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIY 1639
            +LF H  + NCYT    +       S+ G  + L +V    +Y + +      L+   G KI++H+    P I++ G  L  +   SI    T+  +L  P  +C  +       NLY    S KYT + C+ + FQ E++  C C   + P   LPN +  YC       N E       C  K+ + F +  E+     C   C +  +  T SL +W                  SL  +Q       ++ +L   L +            KLNK+L +   NDL  ++I  ++       E    +  TLLS  GG   L +  +   V EI+E  +
Sbjct:  284 TLFHHPLYGNCYTFNSAERGNLLVSSMGGAEYGLKVV----LY-IDEDEYNPYLSTAAGAKILVHDQDEYPFIEDLGTELETATETSIGMQLTESTKLSDPYSDCTMDGSDVSVENLY----SKKYTLQICLNSCFQREMVRTCGCAHYDQP---LPNGA-KYC-------NYEEYPSWIYCYFKLYKQF-VQEELGCQSACRESCSFKEWTLTRSLAKWP-----------------SLNSEQW------MLRVLSWELGE------------KLNKNLTK---NDLANLNIFYQDLNSRSISESPTYNIVTLLSNFGGQLGLWMSCSMICVLEIVEVFF 565          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Xenopus
Match: foxe3 (forkhead box E3 [Source:Xenbase;Acc:XB-GENE-480592])

HSP 1 Score: 62.3882 bits (150), Expect = 1.329e-9
Identity = 67/287 (23.34%), Postives = 113/287 (39.37%), Query Frame = 2
Query:  782 IDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQPNL-YDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIY 1639
            + +L++  G +I+IH P+  P +++ GF++ P    SI   Q +   L      C  +  NL   L  S  YT + C+ + FQ ++   C C    +P P  P +   YC   NY  +       +   EK+ +H    R     Q C ++C  + Y+ +    QW +   K+          F L + + K  + N              FR ++ K        +     +L+Y S+          +E   +    LLS MGG  S   G +   V EI E ++
Sbjct:  299 MPLLSQAAGARIMIHNPNQPPRVEHQGFDIRPGTETSIGVKQDEVRHLGGKYGPCASDGNNLGLKLLYSTSYTLQACLNSCFQYKMTETCGCGYYFYPLP--PGTE--YC---NYNKHPGWGHCFYQLYEKMLDH----RHHCFTQ-CPMQCTKTRYQLSAGTAQWPSPTSKV--------CAFLLLELECKVTLFN--------------FRSDILK--------INVYYEELSYQSV----------EETPAMPVTLLLSNMGGQWSWWFGSSVLSVVEIAELVF 533          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Xenopus
Match: cdh12 (cadherin 12, type 2 (N-cadherin 2) [Source:Xenbase;Acc:XB-GENE-982876])

HSP 1 Score: 56.9954 bits (136), Expect = 6.477e-8
Identity = 58/222 (26.13%), Postives = 93/222 (41.89%), Query Frame = 2
Query:  641 SLFSHQDFFNCYTIEGEQE---YTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQP-----NLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKA 1282
            +LF H  + NCYT    +       S+ G  + L +V    +Y + +      L+   G KI++H+    P I++ G  L  +   SI    T+  +L  P  +C  +       NLY    S KYT + C+ + FQ E++  C C   + P   LPN +  YC       N E       C  K+ + F +  E+     C   C +  +  T SL +W +
Sbjct:  274 TLFHHPLYGNCYTFNSAERGNLLVSSMGGAEYGLKVV----LY-IDEDEYNPYLSTAAGAKILVHDQDEYPFIEDLGTELETATETSIGMQLTESTKLSDPYSDCTMDGSDVSVENLY----SKKYTLQICLNSCFQREMVRTCGCAHYDQP---LPNGA-KYC-------NYEEYPSWIYCYFKLYKQF-VQEELGCQSACRESCSFKEWTLTRSLAKWPS 474          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Mouse
Match: Scnn1g (sodium channel, nonvoltage-gated 1 gamma [Source:MGI Symbol;Acc:MGI:104695])

HSP 1 Score: 60.8474 bits (146), Expect = 3.521e-9
Identity = 85/347 (24.50%), Postives = 137/347 (39.48%), Query Frame = 2
Query:  641 SLFSHQDFFNCYTIEGEQEYT-------KSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQP-----NLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQW--KASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIY 1639
            +LF H  + NCYT    +  T        S  GL  IL I  +++ Y     FL+       G K+++H+ +  P I++ G  +  +   SI    T+ ++L  P   C ++       N+Y+      Y+ + C+ + FQT+++ KC C   + P P   N    YC   NY  +   +     C  ++ + F +  E+     C   C +  +  T SL QW  +ASE+ L                         +N+L      T D  + + K  KLNK+       DL  + I  K+       E        LLS  GG   L +  +   V EIIE  +
Sbjct:  279 TLFHHPMYGNCYTFNNRENATILSTSMGGSEYGLQVILYI--NEDEY---NPFLVS----STGAKVLVHQQNEYPFIEDVGTEIETAMSTSIGMHLTESFKLSEPYSQCTEDGSDVPVTNIYNAA----YSLQICLYSCFQTKMVEKCGCAQYSQPLPPAAN----YC---NYQQHPNWM----YCYYQLYQAF-VREELGCQSVCKQSCSFKEWTLTTSLAQWPSEASEKWL-------------------------LNVL------TWDQSQQINK--KLNKT-------DLAKLLIFYKDLNQRSIMESPANSIEMLLSNFGGQLGLWMSCSVVCVIEIIEVFF 560          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Mouse
Match: Scnn1a (sodium channel, nonvoltage-gated 1 alpha [Source:MGI Symbol;Acc:MGI:101782])

HSP 1 Score: 55.4546 bits (132), Expect = 1.709e-7
Identity = 83/357 (23.25%), Postives = 135/357 (37.82%), Query Frame = 2
Query:  614 QDSISQLKISLFSHQDFFNCYTIEGEQE---YTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQP-----NLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRP-------FLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIY 1639
            Q   +Q   S F H  + NCYT   +     +  S+ G+   L +    E     ++  I +L+   G ++++H       +D+ GFN+ P    SI   +     L     +C +N       NLY      KYT + C+ + FQ  +I KC C    +P+P       +L  SS+ YC                    K+Q  FSL+         SL C +S      S+T +K        L   Y  + S+K   S++ I  ++++                     N   + NK N +  ++I  K        E   +   +LLS +G   SL  G +   V E+ E I+
Sbjct:  288 QAPCNQANYSQFHHPMYGNCYTFNNKNNSNLWMSSMPGVNNGLSLTLRTE-----QNDFIPLLSTVTGARVMVHGQDEPAFMDDGGFNVRPGVETSISMRKEALDSLGGNYGDCTENGSDVPVKNLYPS----KYTQQVCIHSCFQENMIKKCGCAYIFYPKPKGVEFCDYLKQSSWGYCYY------------------KLQAAFSLD---------SLGC-FSKCRKPCSVTNYK--------LSAGYSRWPSVK---SQDWIFEMLSL--------------------QNNYTINNKRNGVAKLNIFFKELNYKTNSESPSVTMVSLLSNLGSQWSLWFGSSVLSVVEMAELIF 576          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Mouse
Match: Scnn1a (sodium channel, nonvoltage-gated 1 alpha [Source:MGI Symbol;Acc:MGI:101782])

HSP 1 Score: 55.4546 bits (132), Expect = 1.709e-7
Identity = 83/357 (23.25%), Postives = 135/357 (37.82%), Query Frame = 2
Query:  614 QDSISQLKISLFSHQDFFNCYTIEGEQE---YTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQP-----NLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRP-------FLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIY 1639
            Q   +Q   S F H  + NCYT   +     +  S+ G+   L +    E     ++  I +L+   G ++++H       +D+ GFN+ P    SI   +     L     +C +N       NLY      KYT + C+ + FQ  +I KC C    +P+P       +L  SS+ YC                    K+Q  FSL+         SL C +S      S+T +K        L   Y  + S+K   S++ I  ++++                     N   + NK N +  ++I  K        E   +   +LLS +G   SL  G +   V E+ E I+
Sbjct:  288 QAPCNQANYSQFHHPMYGNCYTFNNKNNSNLWMSSMPGVNNGLSLTLRTE-----QNDFIPLLSTVTGARVMVHGQDEPAFMDDGGFNVRPGVETSISMRKEALDSLGGNYGDCTENGSDVPVKNLYPS----KYTQQVCIHSCFQENMIKKCGCAYIFYPKPKGVEFCDYLKQSSWGYCYY------------------KLQAAFSLD---------SLGC-FSKCRKPCSVTNYK--------LSAGYSRWPSVK---SQDWIFEMLSL--------------------QNNYTINNKRNGVAKLNIFFKELNYKTNSESPSVTMVSLLSNLGSQWSLWFGSSVLSVVEMAELIF 576          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Mouse
Match: Scnn1a (sodium channel, nonvoltage-gated 1 alpha [Source:MGI Symbol;Acc:MGI:101782])

HSP 1 Score: 55.0694 bits (131), Expect = 1.853e-7
Identity = 83/357 (23.25%), Postives = 135/357 (37.82%), Query Frame = 2
Query:  614 QDSISQLKISLFSHQDFFNCYTIEGEQE---YTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQP-----NLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRP-------FLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIY 1639
            Q   +Q   S F H  + NCYT   +     +  S+ G+   L +    E     ++  I +L+   G ++++H       +D+ GFN+ P    SI   +     L     +C +N       NLY      KYT + C+ + FQ  +I KC C    +P+P       +L  SS+ YC                    K+Q  FSL+         SL C +S      S+T +K        L   Y  + S+K   S++ I  ++++                     N   + NK N +  ++I  K        E   +   +LLS +G   SL  G +   V E+ E I+
Sbjct:  207 QAPCNQANYSQFHHPMYGNCYTFNNKNNSNLWMSSMPGVNNGLSLTLRTE-----QNDFIPLLSTVTGARVMVHGQDEPAFMDDGGFNVRPGVETSISMRKEALDSLGGNYGDCTENGSDVPVKNLYPS----KYTQQVCIHSCFQENMIKKCGCAYIFYPKPKGVEFCDYLKQSSWGYCYY------------------KLQAAFSLD---------SLGC-FSKCRKPCSVTNYK--------LSAGYSRWPSVK---SQDWIFEMLSL--------------------QNNYTINNKRNGVAKLNIFFKELNYKTNSESPSVTMVSLLSNLGSQWSLWFGSSVLSVVEMAELIF 495          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Mouse
Match: Scnn1a (sodium channel, nonvoltage-gated 1 alpha [Source:MGI Symbol;Acc:MGI:101782])

HSP 1 Score: 55.0694 bits (131), Expect = 1.990e-7
Identity = 83/357 (23.25%), Postives = 135/357 (37.82%), Query Frame = 2
Query:  614 QDSISQLKISLFSHQDFFNCYTIEGEQE---YTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQP-----NLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRP-------FLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIY 1639
            Q   +Q   S F H  + NCYT   +     +  S+ G+   L +    E     ++  I +L+   G ++++H       +D+ GFN+ P    SI   +     L     +C +N       NLY      KYT + C+ + FQ  +I KC C    +P+P       +L  SS+ YC                    K+Q  FSL+         SL C +S      S+T +K        L   Y  + S+K   S++ I  ++++                     N   + NK N +  ++I  K        E   +   +LLS +G   SL  G +   V E+ E I+
Sbjct:  207 QAPCNQANYSQFHHPMYGNCYTFNNKNNSNLWMSSMPGVNNGLSLTLRTE-----QNDFIPLLSTVTGARVMVHGQDEPAFMDDGGFNVRPGVETSISMRKEALDSLGGNYGDCTENGSDVPVKNLYPS----KYTQQVCIHSCFQENMIKKCGCAYIFYPKPKGVEFCDYLKQSSWGYCYY------------------KLQAAFSLD---------SLGC-FSKCRKPCSVTNYK--------LSAGYSRWPSVK---SQDWIFEMLSL--------------------QNNYTINNKRNGVAKLNIFFKELNYKTNSESPSVTMVSLLSNLGSQWSLWFGSSVLSVVEMAELIF 495          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. UniProt/SwissProt
Match: sp|P55270|SCNNA_BOVIN (Amiloride-sensitive sodium channel subunit alpha OS=Bos taurus OX=9913 GN=SCNN1A PE=1 SV=2)

HSP 1 Score: 70.4774 bits (171), Expect = 2.140e-11
Identity = 83/354 (23.45%), Postives = 133/354 (37.57%), Query Frame = 2
Query:  614 QDSISQLKISLFSHQDFFNCYTIEGEQE---YTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQP-----NLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKR-----FSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFI 1636
            QDS ++   S F H  + NCYT   +     +  S+ G+   L +    E     ++  I +L+   G ++++HE      +D++GFNL P    SI  S+    RL     +C  N       NLY+     KYT + C+ + FQ  +I +C C    +PRP                +  E  D R       C  K+Q+ FS +R     + C   C  + Y+ + S +QW ++  +    Q L R                                         N   ++NK + +  ++I  K        E   +   TLLS +G   SL  G +   V E+ E I
Sbjct:  267 QDSCNEANYSHFHHPMYGNCYTFNDKNSSNLWMSSMPGVNNGLSLTLRTE-----QNDFIPLLSTVTGARVMVHERDEPAFMDDAGFNLRPGVETSISMSKEAVDRLGGDYGDCTKNGSEVPVENLYNT----KYTQQVCIHSCFQESMIKECGCAYIFYPRP----------------DGVEFCDYRKHNSWGYCYYKLQDAFSSDRLGCFTK-CRKPCSVTIYKLSASYSQWPSATSQDWVFQMLSRQ----------------------------------------NNYTIKNKRDGVAKLNIFFKELNYKSNSESPSVTMVTLLSNLGSQWSLWFGSSVLSVVEMAELI 554          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. UniProt/SwissProt
Match: sp|Q25011|FANA_CORAP (FMRFamide-activated amiloride-sensitive sodium channel OS=Cornu aspersum OX=6535 PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 8.389e-10
Identity = 89/415 (21.45%), Postives = 176/415 (42.41%), Query Frame = 2
Query:  500 TFYMNLSPNESVKVGHS-RDIIRYCIIMKGHDIDVAENCQDSISQLKISLFSHQDFFNCYTIEGEQEYTKSIVG------LIFIL---DIVPSD-EIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQPNLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLK------LNYTN---NSEILDKRF------SCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQ----KQSKENINNVINILKSSL-KDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNF---EISI--KKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFI 1636
             FY NL   ++ K+ H+  D++ +C   +       E C  S      S F   ++FNC+T    Q       G      LIF +   D +P    +Y    + L        G+++V+H P ++P   + G ++ P   +S+        RL  P  NC ++  N     + +KYT   C++   Q  +I +C C  S  P     N++F   +K       N++N   N    D+ F      +C E+ Q++ + +R    +  C   C  ++Y  ++SL+ W     +L  +++ ++      Q    K + E +  + +  +  L ++     + L K + L++  +  + +DL   ++ R N    ++S+   ++        L + +GG   L +G++   + E+IE +
Sbjct:  162 AFYENLG-QDAKKLSHNLEDMLMHCRFNR-------ELCHVS----NFSTFFDGNYFNCFTFNSGQRLQMHATGPENGLSLIFSVEKDDPLPGTYGVYNFDNNIL-----HSAGVRVVVHAPGSMPSPVDHGIDIPPGYSSSVGLKAILHTRLPYPYGNCTNDMLNGI---KQYKYTFFACLQLCKQRLIIQRCGCKSSALPEVPSYNATFCGVIKDWQEINRNHSNEDHNQSEEDRAFIPTPYLACEEREQKNLNNDRTYELSCGCFQPCSETSYLKSVSLSYWPLEFYQLSAVERFFKQERQAGQNHFMKTAYEYLEKLAHPSQKHLARNDSHMDDILSKSYSLSEKEMAKEASDLIRQNMLRLNIYLEDLSVVEYRQLPAYGLADLFADIGGTLGLWMGISVLTIMELIELV 556          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. UniProt/SwissProt
Match: sp|H2QAR5|SCNNG_PANTR (Amiloride-sensitive sodium channel subunit gamma OS=Pan troglodytes OX=9598 GN=SCNN1G PE=3 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.043e-9
Identity = 66/260 (25.38%), Postives = 109/260 (41.92%), Query Frame = 2
Query:  536 KVGHSRDIIRYCIIMKGHDIDVAENCQDSISQLKISLFSHQDFFNCYTI---EGEQEYTKSIVGLIFILDIVPSDEIY---ELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQP-----NLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKA 1282
            KVG S       II K  ++   E+ Q    QL  +LF H    NCYT    E E   + S+ G  + L ++    +Y   E    FL+       G K++IH     P +++ G  +  + + SI    T+ ++L  P   C ++       N+Y+      Y+ + C+ + FQT+++ KC C   + P P   N    YC   NY  +   +     C  ++   F +  E+     C   C +  +  T SL QW +
Sbjct:  181 KVGGS-------IIHKASNVMHIESKQVVGFQLNFTLFHHPMHGNCYTFNNRENETILSTSMGGSEYGLQVI----LYINEEEYNPFLVS----STGAKVIIHRQDEYPFVEDVGTEIETAMVTSIGMHLTESFKLSEPYSQCTEDGSDVPIRNIYNAA----YSLQICLHSCFQTKMVEKCGCAQYSQPLPPAAN----YC---NYQQHPNWM----YCYYQLHRAF-VQEELGCQSVCKEACSFKEWTLTTSLAQWPS 409          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. UniProt/SwissProt
Match: sp|P37091|SCNNG_RAT (Amiloride-sensitive sodium channel subunit gamma OS=Rattus norvegicus OX=10116 GN=Scnn1g PE=1 SV=2)

HSP 1 Score: 62.3882 bits (150), Expect = 6.878e-9
Identity = 86/347 (24.78%), Postives = 138/347 (39.77%), Query Frame = 2
Query:  641 SLFSHQDFFNCYTIEGEQEYT-------KSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQP-----NLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQW--KASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIY 1639
            +LF H  + NCYT   ++  T        S  GL  IL I  +++ Y     FL+       G K++IH+ +  P I++ G  +  +   SI    T+ ++L  P   C ++       N+Y+      Y+ + C+ + FQT+++ KC C   + P P   N    YC   NY  +   +     C  ++ + F +  E+     C   C +  +  T SL QW  +ASE+ L                         +N+L      T D  + + K  KLNK+       DL  + I  K+       E        LLS  GG   L +  +   V EIIE  +
Sbjct:  274 TLFHHPMYGNCYTFNNKENATILSTSMGGSEYGLQVILYI--NEDEY---NPFLVS----STGAKVLIHQQNEYPFIEDVGMEIETAMSTSIGMHLTESFKLSEPYSQCTEDGSDVPVTNIYNAA----YSLQICLYSCFQTKMVEKCGCAQYSQPLPPAAN----YC---NYQQHPNWM----YCYYQLYQAF-VREELGCQSVCKQSCSFKEWTLTTSLAQWPSEASEKWL-------------------------LNVL------TWDQSQQINK--KLNKT-------DLAKLLIFYKDLNQRSIMESPANSIEMLLSNFGGQLGLWMSCSVVCVIEIIEVFF 555          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. UniProt/SwissProt
Match: sp|H2Q5A1|SCNNA_PANTR (Amiloride-sensitive sodium channel subunit alpha OS=Pan troglodytes OX=9598 GN=SCNN1A PE=3 SV=2)

HSP 1 Score: 61.2326 bits (147), Expect = 1.891e-8
Identity = 82/350 (23.43%), Postives = 129/350 (36.86%), Query Frame = 2
Query:  614 QDSISQLKISLFSHQDFFNCYTIEGEQE---YTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQP-----NLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIY 1639
            Q S +Q   S F H  + NCYT   +     +  S+ G+   L ++   E     ++  I +L+   G ++++H       +D+ GFNL P    SI   +    RL     +C  N       NLY      KYT + C+ + FQ  +I +C C    +PRP     +  YC   +Y  +S        C  K+Q  FS +      + C   C  ++Y+ +   ++W +   +    Q L R                                         N   V NK N +  V+I  K        E   +   TLLS +G   SL  G +   V E+ E I+
Sbjct:  309 QVSCNQANYSHFHHPMYGNCYTFNDKNNSNLWMSSMPGINNGLSLMLRAE-----QNDFIPLLSTVTGARVMVHGQDEPAFMDDGGFNLRPGVETSISMRKETLDRLGGDYGDCTKNGSDVPVENLYPS----KYTQQVCIHSCFQESMIKECGCAYIFYPRP----QNVEYC---DYRKHSSW----GYCYYKLQVDFSSDHLGCFTK-CRKPCSVTSYQLSAGYSRWPSVTSQEWVFQMLSRQ----------------------------------------NNYTVNNKRNGVAKVNIFFKELNYKTNSESPSVTMVTLLSNLGSQWSLWFGSSVLSVVEMAELIF 597          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. TrEMBL
Match: A0A4E0RFC3 (FMRFamide-activated amiloride-sensitive sodium channel OS=Fasciola hepatica OX=6192 GN=D915_003846 PE=3 SV=1)

HSP 1 Score: 339.732 bits (870), Expect = 4.375e-105
Identity = 200/526 (38.02%), Postives = 298/526 (56.65%), Query Frame = 2
Query:  107 SKTKLFFQTFSIRGVSKIHHAKSQFEKTLWISXXXXXXXXXXXXXXXXXKNYLQYITTWHTSTKFEDQAAFPGITFCAQNPFSKKSNEIWMRNQILSPTQMQDKXXXXXXXXXXXXXXXXXXXXXXXXXXXTFYMNLSPNESVKVGHSRDIIRYCIIMKGHDIDVAENCQ-DSISQLKISLFSHQDFFNCYTIEGE-QEYTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQPNLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSS---FPYCLKLNYTNNSE----ILDKRFSCVEKVQEHFSLNRE-IIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYR---SYFSLKQKQSKENINNVINILKSSLKDTKDFRENLR--KFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIY 1639
            SK KLF +  SIRGV +I  A++ F +TLWI FVI MT +L ++   L+ +YLQY T+WHT +  +D++ FP IT CA NPFS K+N++W    ++SP Q++  +  +SL  L          + L D F  +Y NLSP +S K+ H  +II +CI+       VAE C+ ++++ +    FSH  +FNC TIEG   E T  I  L+ +  +VPS +I +   +F++D   RG+G+K+VIHE +  P I+  GFN+ P++MN I+Y    W RL TP   C D+QP + DLG ++ Y    C++   Q E+I +C CM S  PRP          PYC  ++  ++ E    +L +R +C     E   + RE II    C  RCR+ TY+T +S+T W+ S  KL      YR    Y +   +  K+ +++ +  +     D+ D + N R   + +             TY+S+ R++F+ ++K+EKLV D+  LLSR+GGL SLCIGLT AF  EIIEFIY
Sbjct:   71 SKIKLFGEISSIRGVQRIFRAETPFIRTLWILFVISMTCLLTVSSFMLIVDYLQYTTSWHTRSLPDDKSEFPAITLCAHNPFSLKANKLWSTGHVISPNQVKSNMAAVSLEMLNQDKVEDSVIMALSDSFEFYYTNLSPEDSQKISHDFEIIPHCILYSPEHTVVAEKCEMENVTAMVRRTFSHPKYFNCLTIEGGGNESTSDISRLVLLAHLVPSSKIVQARSAFIMDTFTRGEGIKVVIHERNTYPQIERYGFNVQPNRMNEIIYETLSWKRLSTPVAPCKDSQPPISDLGVNYTYETSQCLDIATQEEMIRRCGCMNSEWPRPTRAVHGPRLVPYCTSMDRNSSVEVTATVLSERLACSRIFFEELPVIRERIIRAGICIPRCRFFTYDTKLSVTSWEPSPVKLSSYTANYRIVEEYLTEPIESKKKELSSFLQSIIDPTLDSADEQWNKRIGDYKQFGD-------GTYTYISLIRRDFDTTLKEEKLVFDAPILLSRIGGLCSLCIGLTMAFFIEIIEFIY 589          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. TrEMBL
Match: A0A074ZZG9 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_01528 PE=3 SV=1)

HSP 1 Score: 316.235 bits (809), Expect = 6.715e-96
Identity = 190/527 (36.05%), Postives = 289/527 (54.84%), Query Frame = 2
Query:   98 NSKSKTKLFFQTFSIRGVSKIHHAKSQFEKTLWISXXXXXXXXXXXXXXXXXKNYLQYITTWHTSTKFEDQAAFPGITFCAQNPFSKKSNEIWMRNQILSPTQMQDKXXXXXXXXXXXXXXXXXXXXXXXXXXXTFYMNLSPNESVKVGHSRDIIRYCIIMKGHDIDVAENCQDSI-SQLKISLFSHQDFFNCYTIEGEQ-EYTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQPNLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPF---LPNSSFPYCLKLNYT-NNSEI---LDKRFSCVEKVQEHF-SLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENI-NNVINILKSSLK--DTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIY 1639
            ++  + + F +  SIRGV  I  A++ F ++LWI FVI MT +L +   FL+++YL Y T WHT T  +D+ AFP IT CA NPFS ++N  W     L+P Q+  + T ++   L   N +    L L D   TFY NL P     +GH  ++  +C++       VAE C+  + ++L +   SH  +FNC+TIEG+  E TK I  LI ++ +VPS +I E + SF++D   RG+G+  V+HEP   P+I+  G N+ P++MN I+Y    W RL TPK  C D  P + DLG ++ YT   C+    Q +++ +C C+ + +PRP     P +  PYC  LN +  N E+   L +RF C+ +V +    +  E      C  RC Y TYE  +S+T W  +   L    + YR    L +  S  N+ N+ ++   +S++   + DF  + R       S   +    +TY+S+TR+ FE ++  E LV D   L+SR+GGL SLCIGLT AF  E++E+ Y
Sbjct:   54 STPRRPRTFGEISSIRGVQNISRAETPFIRSLWILFVITMTSLLTIFSSFLIRDYLLYETNWHTRTLRDDKTAFPSITLCAHNPFSLEANRFWREGSTLNPKQINSRFTEIATEVLRQHNVAFSINLLLLDSIETFYTNLDPKVRYNIGHKMEMFPHCMVTSESAHIVAEKCKLELDTELHLHRISHPKYFNCWTIEGKALEATKEITRLIMLVQLVPSKKINEANLSFIMDTFTRGEGVISVVHEPGTYPEIEKHGINIQPNRMNQIIYKPVLWNRLATPKAPCSDQPPPISDLGINYTYTQGQCLNTVVQEQLLARCGCLNTEYPRPASTDFPVNPTPYCTSLNESLTNPEMASRLIERFQCIGEVMDQLKKIKEEATRQGRCPPRCSYFTYENRLSVTLWDPNAHNLAAYTERYRKLDGLLKAHSSMNMTNSFVSYWNASMQQFSSLDFESHNRPEQFAEYSQFVD--GSVTYISLTRQEFETTVMDEHLVFDVYILISRIGGLCSLCIGLTMAFFIEVVEYAY 578          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. TrEMBL
Match: H2KR43 (FMRFamide-activated amiloride-sensitive sodium channel OS=Clonorchis sinensis OX=79923 GN=CLF_105403 PE=3 SV=1)

HSP 1 Score: 315.079 bits (806), Expect = 1.943e-95
Identity = 190/531 (35.78%), Postives = 284/531 (53.48%), Query Frame = 2
Query:  101 SKSKTKLFFQTFSIRGVSKIHHAKSQFEKTLWISXXXXXXXXXXXXXXXXXKNYLQYITTWHTSTKFEDQAAFPGITFCAQNPFSKKSNEIWMRNQILSPTQMQDKXXXXXXXXXXXXXXXXXXXXXXXXXXXTFYMNLSPNESVKVGHSRDIIRYCIIMKGHDIDVAENCQDSI-SQLKISLFSHQDFFNCYTIEGEQ-EYTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQPNLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPF---LPNSSFPYCLKLNYT-NNSEILDK---RFSCVEKVQEHF-SLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENI--------NNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIY 1639
            +  K + F +  SIRGV  I  A++ F ++LWI FVI MT +L +   FL+++YL Y T WHT T  +D+ AFP IT CA NPFS ++N  W      +P Q+  + T ++   L   N +    L L D   TFY NL P     +GH  ++  +C++       VAE C+  + ++L +   SH  +FNC+TIEG+  E TK I  LI ++ +VPS +I E + SF++D   RG+G+  V+HEP   P+I+  G N+ P++MN I+Y    W RL TPK  C ++ P + DLG ++ YT   C+    Q +++ +C C+ + +PRP     P +  PYC  LN +  N E+  K   RF C+ +V +    +  E      C  RC Y TYE  +S+T W  +   L    + YR    L + QS  N+        N  I    S   D+ +  E   ++ +     V       TY+S+TR+ FE ++  E+LV D   L+SR+GGL SLCIGLT AF  E++E+ Y
Sbjct:   58 APRKPRTFGEISSIRGVQNISRAETPFIRSLWILFVITMTSLLTIFSSFLIRDYLLYETNWHTRTLRDDKTAFPSITLCAHNPFSLEANRFWREGSTPNPKQINSRFTEIATEVLRQHNVAFSINLLLLDSIETFYTNLDPKVRYNIGHKMEMFPHCMVTSESAHIVAEKCKLELDTELHLHRISHPKYFNCWTIEGKALEATKEITRLIMLVQLVPSKKINEANLSFIMDTFTRGEGVISVVHEPGTYPEIEKHGINIQPNRMNQIIYKPVLWNRLATPKAPCSEHPPPISDLGVNYTYTQGQCLNTVLQEQLLARCGCLNTEYPRPASTDFPVNPTPYCTSLNESLTNPEMASKLIERFQCIGEVMDRLKEIKEEAARQGRCPPRCSYFTYENRLSVTLWDPNAHNLAAYTERYRKLDGLLKAQSSMNMKTSFVSYWNASIQQFSSLDLDSHNQPERFAEYSQFVDGSV-------TYISLTRQEFETTVMDERLVFDVYILISRIGGLCSLCIGLTMAFFIEVVEYAY 581          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. TrEMBL
Match: A0A3R7EZA3 (FMRFamide-activated amiloride-sensitive sodium channel OS=Clonorchis sinensis OX=79923 GN=CSKR_10046s PE=3 SV=1)

HSP 1 Score: 314.309 bits (804), Expect = 3.519e-95
Identity = 187/526 (35.55%), Postives = 289/526 (54.94%), Query Frame = 2
Query:  101 SKSKTKLFFQTFSIRGVSKIHHAKSQFEKTLWISXXXXXXXXXXXXXXXXXKNYLQYITTWHTSTKFEDQAAFPGITFCAQNPFSKKSNEIWMRNQILSPTQMQDKXXXXXXXXXXXXXXXXXXXXXXXXXXXTFYMNLSPNESVKVGHSRDIIRYCIIMKGHDIDVAENCQDSI-SQLKISLFSHQDFFNCYTIEGEQ-EYTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQPNLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPF---LPNSSFPYCLKLNYT-NNSEILDK---RFSCVEKVQEHF-SLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENI-NNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKIND--LTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIY 1639
            +  K + F +  SIRGV  I  A++ F ++LWI FVI MT +L +   FL+++YL Y T WHT T  +D+ AFP IT CA NPFS ++N  W      +P Q+  + T ++   L   N +    L L D   TFY NL P     +GH  ++  +C++       VAE C+  + ++L +   SH  +FNC+TIEG+  E TK I  LI ++ +VPS +I E + SF++D   RG+G+  V+HEP   P+I+  G N+ P++MN I+Y    W RL TPK  C ++ P + DLG ++ YT   C+    Q +++ +C C+ + +PRP     P +  PYC  LN +  N E+  K   RF C+ +V +    +  E      C  RC Y TYE  +S+T W  +   L    + YR    L + QS  N+  + ++   +S++       +L   ++  +    ++  D  +TY+S+TR+ FE ++  E+LV D   L+SR+GGL SLCIGLT AF  E++E+ Y
Sbjct:   58 APRKPRTFGEISSIRGVQNISRAETPFIRSLWILFVITMTSLLTIFSSFLIRDYLLYETNWHTRTLRDDKTAFPSITLCAHNPFSLEANRFWREGSTPNPKQINSRFTEIATEVLRQHNVAFSINLLLLDSIETFYTNLDPKVRYNIGHKMEMFPHCMVTSESAHIVAEKCKLELDTELHLHRISHPKYFNCWTIEGKALEATKEITRLIMLVQLVPSKKINEANLSFIMDTFTRGEGVISVVHEPGTYPEIEKHGINIQPNRMNQIIYKPVLWNRLATPKAPCSEHPPPISDLGVNYTYTQGQCLNTVLQEQLLARCGCLNTEYPRPASTDFPVNPTPYCTSLNESLTNPEMASKLIERFQCIGEVMDRLKEIKEEAARQGRCPPRCSYFTYENRLSVTLWDPNAHNLAAYTERYRKLDGLLKAQSSMNMKTSFVSYWNASIQQFSSL--DLESHNQPERFAEYSQFVDGSVTYISLTRQEFETTVMDERLVFDVYILISRIGGLCSLCIGLTMAFFIEVVEYAY 581          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. TrEMBL
Match: A0A4S2LSS8 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_005099 PE=3 SV=1)

HSP 1 Score: 313.923 bits (803), Expect = 6.343e-95
Identity = 187/526 (35.55%), Postives = 288/526 (54.75%), Query Frame = 2
Query:  101 SKSKTKLFFQTFSIRGVSKIHHAKSQFEKTLWISXXXXXXXXXXXXXXXXXKNYLQYITTWHTSTKFEDQAAFPGITFCAQNPFSKKSNEIWMRNQILSPTQMQDKXXXXXXXXXXXXXXXXXXXXXXXXXXXTFYMNLSPNESVKVGHSRDIIRYCIIMKGHDIDVAENCQDSI-SQLKISLFSHQDFFNCYTIEGEQ-EYTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQPNLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPF---LPNSSFPYCLKLNYT-NNSEILDK---RFSCVEKVQEHF-SLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENI-NNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKIND--LTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIY 1639
            +  K + F +  SIRGV  I  A++ F ++LWI FVI MT +L +   FL+++YL Y T WHT T  +D+ AFP IT CA NPFS ++N  W      +P Q+  + T ++   L   N +    L L D   TFY NL P     +GH  ++  +C++       VAE C+  + ++L +   SH  +FNC+TIEG+  E TK I  LI ++ +VPS +I E + SF++D   RG+G+  V+HEP   P+I+  G N+ P++MN I+Y    W RL TPK  C D+ P + DLG ++ YT   C+    Q +++ +C C+ + +PRP     P +  PYC  LN +  N E+  K   RF C+ +V +    +  E      C  RC Y TYE  +S+T W  +   L    + YR    L +  S  N+  + ++   +S++       +L   ++  +    ++  D  +TY+S+TR+ FE ++  E+LV D   L+SR+GGL SLCIGLT AF  E++E+ Y
Sbjct:   65 APRKPRTFGEISSIRGVQNISRAETPFIRSLWILFVITMTSLLTIFSSFLIRDYLLYETNWHTRTLRDDKTAFPSITLCAHNPFSLEANRFWREGSTPNPKQINSRFTEIATEVLRQRNVAFSINLLLLDSIETFYTNLDPKVRYNIGHKMEMFPHCMVTSESAHIVAEKCKLELDTELHLHRISHPKYFNCWTIEGKALEATKEITRLIMLIQLVPSKKINEANLSFIMDTFTRGEGVISVVHEPGTYPEIEKHGINIQPNRMNQIIYKPVLWNRLATPKAPCSDHPPPISDLGVNYTYTQGQCLNTVLQEQLLARCGCLNTEYPRPASTDFPVNPTPYCTSLNESLTNPEMASKLIERFQCIGEVMDRLKEIKEEAARQGRCLPRCSYFTYENRLSVTLWDPNAHNLAAYTERYRKLDGLLKAHSSMNLTTSFVSYWNASIQQFSSL--DLESHNQPERFAEYSQFVDGSVTYISLTRQEFETTVMDERLVFDVYILISRIGGLCSLCIGLTMAFFIEVVEYAY 588          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Sea Lamprey
Match: SCNN1G (sodium channel epithelial 1 gamma subunit [Source:HGNC Symbol;Acc:HGNC:10602])

HSP 1 Score: 53.5286 bits (127), Expect = 1.003e-7
Identity = 48/182 (26.37%), Postives = 78/182 (42.86%), Query Frame = 2
Query:  791 LNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQPN-----LYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYR 1321
            L  G G KI IH  +  P I+  G ++ P+  +S+     D  +L  P  +C  +  +     LYD      Y+ + C  + FQ E+I  C C  +N+ +P    S F      NY NN         C  ++ + + +  E+   Q C   C  + +E T+SL  W +   K   L+ L +
Sbjct:  315 LAMGAGAKIGIHLQNTTPFIEAVGIDIPPAMESSLGLRVNDVQKLGDPYSDCTMDGSDIDVKSLYD----SPYSVQTCQNSCFQWEMIKSCGC--ANYEQPLPEGSRF-----CNYDNNP----GWEYCYYRLYDMY-IKEELKCIQVCRQICSETEHEVTLSLADWPSKASKGWLLRALSK 480          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Sea Lamprey
Match: SCNN1A (sodium channel epithelial 1 alpha subunit [Source:HGNC Symbol;Acc:HGNC:10599])

HSP 1 Score: 50.447 bits (119), Expect = 8.343e-7
Identity = 59/254 (23.23%), Postives = 101/254 (39.76%), Query Frame = 2
Query:  611 CQDSISQLKISLFSHQDFFNCYTIEGEQE---YTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHD---NQP--NLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQF-----CSLRCRYSTYETTMSLTQW--KASEQKLEHLQKLYRSY 1327
            C DS    + S F H  + NCYT         +  S  G  + L ++   E     ++  I  L+   G +I++H+  + P ++  GF++ P    S+     +  R+  P  NC +   N P  NLY    + +     C    FQ  ++  C C    +P P  PN+S  YC   +Y N +               ++ L R+ I ++      C+  C    +  T     W   +SE+ + +L  L  +Y
Sbjct:  225 CSDS----EYSRFHHPTYGNCYTFNSANSSKLWQASKPGRDYGLSLILRTE-----QNDYIPFLSTVAGARIMVHDQESPPFMEEGGFDMRPGFETSLGIRMLEATRMPDPYGNCTEDGSNVPVLNLYSSAYTVQVPECSC----FQLALVEACGCGYYFYPLP--PNAS--YC---SYNNTA----------WAGHCYYKLYRQFISDELGCVDKCAQPCTTKRFAVTPGYAAWPDSSSEKWIFNLLSLQNNY 448          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Sea Lamprey
Match: SCNN1B (sodium channel epithelial 1 beta subunit [Source:HGNC Symbol;Acc:HGNC:10600])

HSP 1 Score: 49.6766 bits (117), Expect = 1.613e-6
Identity = 59/261 (22.61%), Postives = 107/261 (41.00%), Query Frame = 2
Query:  617 DSISQLKISLFSHQDFFNCYTIE-GEQEYTKSI--VGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQPNL--YDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQW--KASEQKLEHLQKLYRSYFSLKQKQSKENINNVIN 1378
            D+ S    + F H  + NC+    GE E    +   G+ + L +V S     + +   I  L    G  I++H+ +  P + + G  +      S+        R   P  +C  +  +L   +L     Y+ + C+ + FQT++I  C C    +P P  P     YC   N+T        R+ C  K+ E F +  ++     C   C  S Y+ ++S++ W  ++SE  + H+            K+ K N++ + N
Sbjct:  258 DTCSATNFTTFFHPMYGNCFIFNWGENETVMQVSNPGVEYGLKLVLS-----IDQDEYIPFLTTIAGAVIMVHDQNTYPFLSDLGVFVKTGVETSVGIEVGQLQRQGAPYSDCTMDGTDLPITNLYNGTAYSVQACLRSCFQTKMIEMCGCGYYLYPLP--PGEK--YCQNQNFTG------WRY-CYYKLYEQF-VEEDMDCYTICKQPCIESEYKMSISMSDWPSQSSEDWIFHILS----------KERKHNVSRIFN 491          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Nematostella
Match: EDO42137 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2F2])

HSP 1 Score: 50.447 bits (119), Expect = 1.397e-6
Identity = 64/285 (22.46%), Postives = 115/285 (40.35%), Query Frame = 2
Query:  806 GMKIVIHEPHNLPDIDNSGFNLHPSK-----MNSIVYSQTDWYRLRTPKVNCHDNQPNLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHF---SLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFI 1636
            GMK+ I +   LP     G  + P +     M  +   + D ++ R+ + +   +  NL+  G +  Y+   C  +      I +C C  +N+  P +   + P C +L    NS I+D    C+ K  + F   S +RE      C   C   +++ T+S  +W A   ++      Y  +  ++          V+N + SS        EN+ + H            +L Y+ +          +E L     +LLS +GG   + IG++A   AE+IE +
Sbjct:  250 GMKVTISDQGVLPFPGQQGIRIMPGQSTGIQMTKLQTRRIDPFKNRSCESSNEMSDKNLF-FGYNMTYSIMACKYSCLNARKIERCGC--TNYNTPEMQKRNIPLCNRL----NSTIID----CLNKAYDTFEDGSCDRE------CPPSCSEVSFDLTVSSARWPARSFEVTK----YAYFLRVR----------VLNKIASSSSKYFVNSENIAQVHVY--------YGELDYLLV----------QETLAYTFMSLLSDIGGQMGMWIGISALTCAELIELV 485          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Nematostella
Match: EDO29539 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T3C7])

HSP 1 Score: 47.3654 bits (111), Expect = 8.136e-6
Identity = 50/221 (22.62%), Postives = 80/221 (36.20%), Query Frame = 2
Query:  638 ISLFSHQDFFNCYTIEGEQEYT------KSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTP-KVNCHDNQPNLYDLGRSF--KYTHKHCMENQFQTEVINKCQCM-LSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIE--NQFCSLRCRYSTYETTMS 1264
             S F H  + +C+T   + ++         I GL   L++   D +         ++     G K+++H+P   P I+ +GF L P            +  LR P +  C +   N+ D  R F   YT   C +       I KC C  +      F+ + S  +CL L                          RE  E   + C + C Y+ YET +S
Sbjct:   22 FSTFEHLRYGSCHTFNSDGQFGLKVDSIGPISGLQLRLNVEEKDHV--------SNLYGLQAGFKVLVHDPREHPMIEETGFALQPGTHTFCSVRMKKYVNLRAPYRTECGE---NITDFNRYFNVNYTMAICSKQCLHDYGIKKCGCQPIFEITSEFMDSGSLTHCLLLG----------------------PYPREAAECFKKSCPVPCYYTKYETKLS 209          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Nematostella
Match: EDO30893 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZH4])

HSP 1 Score: 47.3654 bits (111), Expect = 8.235e-6
Identity = 62/281 (22.06%), Postives = 106/281 (37.72%), Query Frame = 2
Query:  806 GMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTP-KVNCHDNQPNLYDLGRSFK--YTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLK-LNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFI 1636
            G K+++H+    P ++ +GF L P            +  L TP    C +   N+ D  R F   Y+   C +       I KC C      +P  P +   YC K + + N  +IL   F+      E F         ++C + C Y+ YET +S                 Y S  S          N +   L + L ++KD  +  +     N + +     +L+Y  I          ++K   DS +L+  +GG   L +G++   V E ++ +
Sbjct:   49 GFKVLVHDRKEYPIMEQTGFALQPGTHTFCSTKLKKYKNLPTPYNTKCGE---NITDFNRYFSVNYSMSTCGKQCLHDYGIKKCGC------QPIYPITMKLYCQKRIVFKNAPKILIFLFA-----AECF--------KKYCPVPCDYTKYETKLS-----------------YASAIS----------NALARSLPTHLTESKDVADAFK-----NVATLDVYFEELSYDKI----------EQKPSFDSWSLIGNIGGYLGLFLGMSVLTVMEFLDLL 265          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Planmine SMEST
Match: SMESG000035185.1 (SMESG000035185.1)

HSP 1 Score: 980.319 bits (2533), Expect = 0.000e+0
Identity = 554/555 (99.82%), Postives = 555/555 (100.00%), Query Frame = 2
Query:   80 MIELSENSKSKTKLFFQTFSIRGVSKIHHAKSQFEKTLWISXXXXXXXXXXXXXXXXXKNYLQYITTWHTSTKFEDQAAFPGITFCAQNPFSKKSNEIWMRNQILSPTQMQDKXXXXXXXXXXXXXXXXXXXXXXXXXXXTFYMNLSPNESVKVGHSRDIIRYCIIMKGHDIDVAENCQDSISQLKISLFSHQDFFNCYTIEGEQEYTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQPNLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIYXXXXXXXXXXXXXXXXXXXXXXXXTGSNNLNASVE 1744
            MIELSENSKSKTKLFFQTFSIRGVSKIHHAKSQFEKTLWISFVIIMTFILILTMIFLVKNYLQYITTWHTSTKFEDQAAFPGITFCAQNPFSKKSNEIWMRNQILSPTQMQDKLTLLSLNALENLNNSLLSYLRLYDLFSTFYMNLSPNESVKVGHSRDIIRYCIIMKGHDIDVAENCQDSISQLKISLFSHQDFFNCYTIEGEQEYTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQPNLYDLGRSFKYTHKHCME+QFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIYIICKCNKSRCSKSRIRRKRNCSNSTGSNNLNASVE
Sbjct:    1 MIELSENSKSKTKLFFQTFSIRGVSKIHHAKSQFEKTLWISFVIIMTFILILTMIFLVKNYLQYITTWHTSTKFEDQAAFPGITFCAQNPFSKKSNEIWMRNQILSPTQMQDKLTLLSLNALENLNNSLLSYLRLYDLFSTFYMNLSPNESVKVGHSRDIIRYCIIMKGHDIDVAENCQDSISQLKISLFSHQDFFNCYTIEGEQEYTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQPNLYDLGRSFKYTHKHCMESQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIYIICKCNKSRCSKSRIRRKRNCSNSTGSNNLNASVE 555          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Planmine SMEST
Match: SMESG000081715.1 (SMESG000081715.1)

HSP 1 Score: 501.901 bits (1291), Expect = 5.380e-172
Identity = 268/518 (51.74%), Postives = 371/518 (71.62%), Query Frame = 2
Query:  107 SKTKLFFQTFSIRGVSKIHHAKSQFEKTLWISXXXXXXXXXXXXXXXXXKNYLQYITTWHTSTKFEDQAAFPGITFCAQNPFSKKSNEIWMRNQILSPTQMQDKXXXXXXXXXXXXXXXXXXXXXXXXXXXTFYMNLSPNESVKVGHSRDIIRYCIIMKGHDIDVAENCQDSISQLKISLFSHQDFFNCYTIEGEQEYTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQPNLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKI-------NDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIY 1639
            SKTKLF Q  SIRG+SKIH AK++FE+ LW  FVI+MT +L + M+FL+++YLQY T WHTST+ +  + FP IT CAQNPFS  +N +W + +I+SP+++QD LT++SLNA ++ N   L YLRLYDLFSTFYMNL   +++K+GHS+D+IRYCI++  +DI +AE C     +  I LFSHQ++FNCYTIEG ++YT  ++GLI ++ I+PS++I ELS SFL+D+L RG+G+K+ +H+P   PDI+NSG N+HP+KMNS+VYS T W R+ T K  C D Q  + DL R FKY    C+  + Q ++I +C CMLSN PRP+ PN + PYC+KL     ++++ +R SCVE +Q      R+ I+N  C L+C+++TYETTMSLT+W A+E KL++L+ LYRSYF+ +   S ENI  + +IL +SL  + + R      H++NK+   N I       N  TY+S+ R+NF+I  K EKLVLDSNTL SRMGGL+S+CIGLTAA  AE IEF+Y
Sbjct:    2 SKTKLFCQISSIRGISKIHSAKTKFERFLWTGFVILMTCVLFVQMVFLIQDYLQYTTAWHTSTELDYPSPFPAITLCAQNPFSIDANRLWSKRKIMSPSELQDNLTIMSLNAFDDKNLDGLEYLRLYDLFSTFYMNLPFLDTIKLGHSKDMIRYCILIYKNDIIIAEKCDLKNEKFNIKLFSHQEYFNCYTIEGLEQYTTDVIGLILLVQIIPSEKIPELSNSFLVDILQRGEGLKVAVHQPQTYPDIENSGINMHPAKMNSLVYSPTKWIRMSTSKNPCRDKQKPINDLNRHFKYAFTQCVSAEIQRQIIKQCGCMLSNAPRPYPPNETIPYCIKLVSPTKAKLILERLSCVENIQSQSVEIRKTIQNDLCFLKCQFTTYETTMSLTRWTANELKLKNLENLYRSYFAYRHNSSSENIVKIKSILLNSLNRSHNLR------HQINKNQFNNLINYSRFDENAFTYISLMRRNFDIQWKTEKLVLDSNTLFSRMGGLTSICIGLTAAVFAEFIEFLY 513          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Planmine SMEST
Match: SMESG000064696.1 (SMESG000064696.1)

HSP 1 Score: 317.39 bits (812), Expect = 3.081e-100
Identity = 191/546 (34.98%), Postives = 306/546 (56.04%), Query Frame = 2
Query:   32 LYSIRSHELSQKNRKKMIELSENSKSKTKLFFQTFSIRGVSKIHHAKSQFEKTLWISXXXXXXXXXXXXXXXXXKNYLQYITTWHTSTKFEDQAAFPGITFCAQNPFSKKSNEIWMRNQILSPTQMQDKXXXXXXXXXXXXXXXXXXXXXXX-XXXXTFYMNLSPNESVKVGHSRDIIRYCIIMKGHDIDVAENCQDSISQL--KISLFSHQDFFNCYTIEGEQEYT-KSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQPNLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQF----CSLRCRYSTYETTMSLTQWKASEQKLEHLQKLY-RSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVR-NKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIY 1639
            + S     + Q +RK    +    K+   LF +  SIRG+ KI  A + F K LWI+FV  MT +L L    L+K+YL Y T WH  TKF+D + FP IT C+ NPFS ++N +W +    +P   +++L  + ++ +    +     L ++ D    +YMN+  + S+K+ H  ++++YC I     I +  NC+    ++  KI +FSHQ FFNCYTI+    Y+ ++I  LI ++ + P+ +I EL  SFL+D+L RG+G+K+VIHEP++ P+I+  G ++ P KMN+I Y    W R  TP   C + Q  ++DL R F Y+   CM    Q ++I  C CM +  PRPF+PN    YC+ L + N+S+I         K  + F++N + ++ Q+    C  +C Y TY T +S+T+W  S +KL  +  L  ++   LK  + +E+  + I+ +++++ ++            +N S +     N  TY+S++RK     IK E+LVLD   L+SR+GGL SL IGLT +F  E++EF Y
Sbjct:    1 MISTEKETIKQASRKSESNVLVIDKNAFNLFCEISSIRGMHKITKAPTLFLKVLWIAFVFCMTSLLFLVTGLLIKDYLNYYTAWHVRTKFDDNSEFPAITLCSHNPFSLRANSLWKKPNAPNPEMFRERLKQVVIDEVNTTKDPNELTLMMFHDKIENYYMNIDYDFSLKISHGTEMLKYCAIFSDGLIIITYNCETEKDKVVEKI-VFSHQKFFNCYTIQQTSNYSQQTITSLIILVSLTPAADIPELKNSFLMDILERGEGLKLVIHEPNSYPEIEEEGIDVQPGKMNNIKYEPVKWARHSTPPQKCKNGQSPIFDLKRQFNYSFSFCMNYNLQLKLIETCGCMNALFPRPFIPNEKIGYCMNLTHRNSSQIFTNL-----KCAQDFTMNVKTLKKQWENTKCMQKCNYFTYNTELSITKWVPSYEKLFRIADLINKTEIYLKNHRERESKEDYISYIQANITNS-----------YINSSFLDLENENKFTYISLSRKTRNTVIKDEELVLDLAILMSRIGGLCSLFIGLTVSFFIELVEFGY 529          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Planmine SMEST
Match: SMESG000016707.1 (SMESG000016707.1)

HSP 1 Score: 310.457 bits (794), Expect = 2.731e-97
Identity = 191/533 (35.83%), Postives = 303/533 (56.85%), Query Frame = 2
Query:  107 SKTKLFFQTFSIRGVSKIHHAKSQFEKTLWISXXXXXXXXXXXXXXXXXKNYLQYITTWHTSTKFEDQAAFPGITFCAQNPFSKKSNEIWMRNQILSPTQMQDKXXXXXXXXXXXXXXXXXXXXXXXXXXXTFYMNLSPNESVKVGHSRDIIRYCIIMKGHDIDVAENC-QDSISQLKISLFSHQDFFNCYTIEGEQEY----------TKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQPNLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCV-EKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSY------FSLKQKQSKENI----NNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIY 1639
            SK  +F +  S+RG+ KIH +K+ F K LWI+FV  M+ +LI+    LV ++  Y T WH ST+ +++  FP I+ C+ NPFSK+++  W   +IL P + +++L  + +  +++ N    + L   +    +Y N+    S+K+GH R I++YC++       +AE+C  ++ +   +S FSH ++FNC+TIE + E           T   V LI ++++    ++++ ++SF++D L RG+G++IVIH   + PDID +G N+ P KMN + +    W R  +PK  C D+QP + DL R + YT   C++     E++ KCQCM +  PRP LP+   PYC   +Y+ N   + KR  CV E+V +     +       C   C ++TY++T+S+T+WK S  KL  + K YRS        SL  K + E I    +N IN LK  LK    ++  L   HK  +    +++N   Y+++ RKNF+ + K EKL +D    +SR+GGL SLCIGLT AF  EIIEF+Y
Sbjct:   51 SKFAIFCEASSVRGMQKIHKSKTPFLKALWIAFVFFMSILLIIISTNLVIDFRNYHTAWHVSTELDEKTRFPAISICSHNPFSKQASRYWKEGKILPPEKFRERLFEVEMELIKSGNLPGQTLLDKAEE---YYGNVDYPSSIKLGHVRKILQYCVVQNDGFTIIAEDCLVENETGFTVSQFSHYNYFNCFTIEAKSEKNGLIKSYGDKTNETVSLILLINVNLFSDLFQANESFVMDSLTRGEGLRIVIHRVGSYPDIDKNGINIQPGKMNEVNFQNIHWTRYSSPKRECKDDQPPIVDLDRIYDYTFAQCLDAIVNKELVEKCQCMSTIWPRPQLPSKDVPYC--NDYSGNLTTIVKRTQCVYEEVFQKLKPIKAAAVGTKCWPLCTFNTYDSTLSITRWKPSLLKLRQITKRYRSVNKYIDDISL-AKNNTETIEQLKSNYINELKRELK----YKPTLSH-HKSEEIFNEHQMN---YINLVRKNFDTTKKIEKLFIDIYIFISRVGGLCSLCIGLTTAFFIEIIEFVY 569          
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Planmine SMEST
Match: SMESG000045131.1 (SMESG000045131.1)

HSP 1 Score: 295.434 bits (755), Expect = 3.686e-92
Identity = 185/541 (34.20%), Postives = 304/541 (56.19%), Query Frame = 2
Query:   59 SQKNRKKMIELSENSKSKTKLFFQTFSIRGVSKIHHAKSQFEKTLWISXXXXXXXXXXXXXXXXXKNYLQYITTWHTSTKFEDQAAFPGITFCAQNPFSKKSNEIWMRNQILSPTQMQDKXXXXXXXXXXXXXXXXXXXXXXXXXXXTFYMNLSPNESVKVGHSRDIIRYCIIMKGHDIDVAENCQ-DSISQLKISLFSHQDFFNCYTIEGEQE----------YTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHEPHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQPNLYDLGRSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTNNSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWKASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLRKFHKLNKSLVRNKIND---LTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGLSSLCIGLTAAFVAEIIEFIY 1639
            S  N  K   L+    S   LF +  SIRG+ KI  A + F +++WI +VI+MT +L +T  FL +++  Y T WHTST F+++  FP I+ C+ NPFS+ +N +W  N +++P + ++   LL+  ++  + N+      LYD    +Y N+    SV + H  +I+RYC++     + +AE C   S    ++  FS+  +FNC+TIE + E           T+ ++ +I ++ I P  ++ +++ SF++D L RG G+K+V+HE    P+I+N G NL P KMN I +    W R  +PK  C +NQ N+ DLG S+KYT   C+E + Q   I  C CM SN PRP  P+ + PYC   N++ +++ + KR+ C   +++     +++++ + C   C Y TY+T +S+T WK S  KL  + ++Y+   +    ++ E     +  LK   +DT            ++K+L  N + D    TY+++ RKNF+   K E+LV+D++  +SR+GGL SL +GLT A  AE+IEF Y
Sbjct:   68 SGYNEPKQKPLANPQPSPLTLFCEAISIRGMQKIARAPNNFLRSVWIGYVILMTTLLTVTSYFLFRDFFNYNTAWHTSTAFDEKTEFPAISICSHNPFSENANRMWQENLVMTPKKFEE---LLTNVSINMVKNNRGGGEILYDSAEYYYGNIEYESSVNLSHKEEILRYCVLTTDAYVYIAEQCDLASYVAYRLRKFSNSKYFNCFTIEEDPEKLKTHSADVVSTRELLSIIILIQITPESDLTQINSSFVMDTLTRGTGVKVVLHEVGTSPEIENYGINLQPGKMNEISFETIHWNRYNSPKRPCLNNQSNIIDLGNSYKYTMSKCVEAELQKSTIKNCGCMNSNWPRPVKPSQNIPYC--TNFSCSTDEILKRYEC-SSIKDQGLTKQKLVDTK-CFGVCSYFTYDTKVSVTNWKPSLYKLNRVTQIYK-LANENYTETDEGKTERVKKLKEKPQDT------------ISKNL--NMLTDESRFTYINVVRKNFDTIKKTERLVIDASIFMSRVGGLCSLLVGLTTAVFAELIEFCY 586          
The following BLAST results are available for this feature:
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
SCNN1A4.291e-923.14sodium channel epithelial 1 alpha subunit [Source:... [more]
SCNN1A5.515e-923.14sodium channel epithelial 1 alpha subunit [Source:... [more]
SCNN1A8.481e-923.14sodium channel epithelial 1 alpha subunit [Source:... [more]
SCNN1G1.866e-824.44sodium channel epithelial 1 gamma subunit [Source:... [more]
SCNN1D9.464e-820.00sodium channel epithelial 1 delta subunit [Source:... [more]
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
asic-21.146e-724.39Degenerin-like protein asic-2 [Source:UniProtKB/S... [more]
unc-1054.787e-720.85Degenerin-like protein unc-105 [Source:UniProtKB/... [more]
unc-1054.787e-720.85Degenerin-like protein unc-105 [Source:UniProtKB/... [more]
unc-1055.121e-720.85Degenerin-like protein unc-105 [Source:UniProtKB/... [more]
unc-1055.121e-720.85Degenerin-like protein unc-105 [Source:UniProtKB/... [more]
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
copg21.242e-1323.44coatomer protein complex subunit gamma 2 [Source:X... [more]
copg22.971e-1223.15coatomer protein complex subunit gamma 2 [Source:X... [more]
cdh121.088e-924.63cadherin 12, type 2 (N-cadherin 2) [Source:Xenbase... [more]
foxe31.329e-923.34forkhead box E3 [Source:Xenbase;Acc:XB-GENE-480592... [more]
cdh126.477e-826.13cadherin 12, type 2 (N-cadherin 2) [Source:Xenbase... [more]
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Scnn1g3.521e-924.50sodium channel, nonvoltage-gated 1 gamma [Source:M... [more]
Scnn1a1.709e-723.25sodium channel, nonvoltage-gated 1 alpha [Source:M... [more]
Scnn1a1.709e-723.25sodium channel, nonvoltage-gated 1 alpha [Source:M... [more]
Scnn1a1.853e-723.25sodium channel, nonvoltage-gated 1 alpha [Source:M... [more]
Scnn1a1.990e-723.25sodium channel, nonvoltage-gated 1 alpha [Source:M... [more]
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P55270|SCNNA_BOVIN2.140e-1123.45Amiloride-sensitive sodium channel subunit alpha O... [more]
sp|Q25011|FANA_CORAP8.389e-1021.45FMRFamide-activated amiloride-sensitive sodium cha... [more]
sp|H2QAR5|SCNNG_PANTR2.043e-925.38Amiloride-sensitive sodium channel subunit gamma O... [more]
sp|P37091|SCNNG_RAT6.878e-924.78Amiloride-sensitive sodium channel subunit gamma O... [more]
sp|H2Q5A1|SCNNA_PANTR1.891e-823.43Amiloride-sensitive sodium channel subunit alpha O... [more]
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4E0RFC34.375e-10538.02FMRFamide-activated amiloride-sensitive sodium cha... [more]
A0A074ZZG96.715e-9636.05Uncharacterized protein OS=Opisthorchis viverrini ... [more]
H2KR431.943e-9535.78FMRFamide-activated amiloride-sensitive sodium cha... [more]
A0A3R7EZA33.519e-9535.55FMRFamide-activated amiloride-sensitive sodium cha... [more]
A0A4S2LSS86.343e-9535.55Uncharacterized protein OS=Opisthorchis felineus O... [more]
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3
Match NameE-valueIdentityDescription
SCNN1G1.003e-726.37sodium channel epithelial 1 gamma subunit [Source:... [more]
SCNN1A8.343e-723.23sodium channel epithelial 1 alpha subunit [Source:... [more]
SCNN1B1.613e-622.61sodium channel epithelial 1 beta subunit [Source:H... [more]
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3
Match NameE-valueIdentityDescription
EDO421371.397e-622.46Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO295398.136e-622.62Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO308938.235e-622.06Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000035185.10.000e+099.82SMESG000035185.1[more]
SMESG000081715.15.380e-17251.74SMESG000081715.1[more]
SMESG000064696.13.081e-10034.98SMESG000064696.1[more]
SMESG000016707.12.731e-9735.83SMESG000016707.1[more]
SMESG000045131.13.686e-9234.20SMESG000045131.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031176 ID=SMED30031176|Name=FMRFamide-activated amiloride-sensitive sodium channel|organism=Schmidtea mediterranea sexual|type=transcript|length=1764bp
TTTGTATAAAAATAAATTCCCATATTAAATTCTCTACAGCATTCGTTCAC
ACGAATTAAGTCAAAAAAATAGAAAAAAAATGATAGAATTATCAGAAAAT
TCTAAATCGAAAACCAAACTATTTTTCCAGACTTTCTCCATTAGGGGAGT
CTCAAAAATTCATCACGCAAAAAGTCAATTTGAAAAAACTCTCTGGATTT
CCTTTGTGATAATCATGACATTTATTTTGATTTTGACAATGATCTTCCTG
GTAAAAAATTATCTCCAATACATCACGACTTGGCACACGTCGACAAAATT
CGAGGATCAGGCAGCATTTCCTGGAATAACGTTTTGTGCTCAAAATCCTT
TCTCTAAGAAATCCAACGAAATTTGGATGAGAAATCAGATTTTATCTCCG
ACACAAATGCAAGACAAATTAACTTTGCTTTCTTTGAATGCACTCGAAAA
TCTCAACAATTCGCTTTTATCTTATCTTCGACTGTATGACTTGTTTAGTA
CTTTTTATATGAATCTGTCTCCGAATGAATCTGTCAAAGTCGGTCATTCG
AGGGACATAATCCGATACTGTATCATCATGAAAGGACATGATATAGATGT
TGCAGAGAATTGTCAAGACAGTATTAGTCAACTGAAAATCAGTTTGTTCT
CCCATCAGGACTTTTTCAATTGTTATACCATCGAAGGTGAACAGGAATAC
ACAAAGTCCATTGTTGGGTTAATCTTTATATTAGACATCGTTCCTTCTGA
CGAGATTTATGAATTATCTAAAAGTTTTTTAATTGACATGTTGAATAGAG
GAGACGGAATGAAAATAGTTATCCACGAACCTCATAACTTGCCCGACATT
GACAATTCTGGATTTAATCTTCACCCATCAAAAATGAATAGCATCGTCTA
CTCTCAAACCGATTGGTATAGGTTACGAACTCCCAAAGTAAATTGCCATG
ACAACCAACCGAATCTCTACGATCTTGGTCGATCATTCAAATATACTCAT
AAACATTGCATGGAAAACCAATTTCAAACTGAAGTCATAAATAAATGCCA
ATGCATGTTGAGCAATCATCCGAGACCGTTTCTTCCAAATTCATCCTTTC
CTTACTGCTTGAAATTAAATTATACTAACAATAGTGAAATTCTAGACAAG
AGATTTAGTTGCGTTGAAAAGGTTCAAGAACATTTTTCTTTGAACCGAGA
AATAATCGAAAATCAGTTCTGTAGTCTTCGATGTCGGTATTCGACGTATG
AGACGACAATGTCTCTGACTCAATGGAAAGCCAGTGAACAAAAGCTTGAA
CATTTGCAAAAGCTTTACCGATCGTATTTCAGCCTTAAACAAAAACAGTC
TAAAGAAAACATCAACAACGTGATTAATATTCTCAAATCTTCTTTGAAAG
ATACAAAGGATTTTCGAGAGAATTTGAGAAAATTCCATAAACTTAACAAA
TCCCTTGTACGCAACAAAATAAATGACTTAACCTACGTCAGTATAACGAG
AAAGAATTTCGAAATTAGCATTAAAAAGGAAAAGTTGGTTTTGGATTCGA
ACACGTTGCTATCGAGAATGGGTGGACTTAGTTCTCTTTGTATCGGTTTG
ACTGCGGCGTTCGTGGCCGAGATCATTGAGTTTATTTATATTATTTGCAA
ATGTAACAAAAGCCGGTGTTCGAAGTCCAGAATTCGAAGGAAGAGGAATT
GCTCCAACAGCACAGGCAGTAATAACTTGAACGCATCTGTCGAATAGCCG
ATTAGAAGTTTCAC
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protein sequence of SMED30031176-orf-1

>SMED30031176-orf-1 ID=SMED30031176-orf-1|Name=SMED30031176-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=556bp
MIELSENSKSKTKLFFQTFSIRGVSKIHHAKSQFEKTLWISFVIIMTFIL
ILTMIFLVKNYLQYITTWHTSTKFEDQAAFPGITFCAQNPFSKKSNEIWM
RNQILSPTQMQDKLTLLSLNALENLNNSLLSYLRLYDLFSTFYMNLSPNE
SVKVGHSRDIIRYCIIMKGHDIDVAENCQDSISQLKISLFSHQDFFNCYT
IEGEQEYTKSIVGLIFILDIVPSDEIYELSKSFLIDMLNRGDGMKIVIHE
PHNLPDIDNSGFNLHPSKMNSIVYSQTDWYRLRTPKVNCHDNQPNLYDLG
RSFKYTHKHCMENQFQTEVINKCQCMLSNHPRPFLPNSSFPYCLKLNYTN
NSEILDKRFSCVEKVQEHFSLNREIIENQFCSLRCRYSTYETTMSLTQWK
ASEQKLEHLQKLYRSYFSLKQKQSKENINNVINILKSSLKDTKDFRENLR
KFHKLNKSLVRNKINDLTYVSITRKNFEISIKKEKLVLDSNTLLSRMGGL
SSLCIGLTAAFVAEIIEFIYIICKCNKSRCSKSRIRRKRNCSNSTGSNNL
NASVE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002028dorsal-ventral muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR001873ENaC
Vocabulary: biological process
TermDefinition
GO:0006814sodium ion transport
GO:0006811ion transport
GO:0035725sodium ion transmembrane transport
GO:0006810transport
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: molecular function
TermDefinition
GO:0005272sodium channel activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001873Epithelial sodium channelPRINTSPR01078AMINACHANNELcoord: 76..92
score: 40.47
coord: 316..333
score: 32.44
coord: 305..316
score: 42.0
coord: 186..202
score: 29.88
coord: 381..401
score: 28.95
coord: 243..261
score: 28.42
IPR001873Epithelial sodium channelPFAMPF00858ASCcoord: 15..518
e-value: 2.1E-56
score: 191.8
IPR001873Epithelial sodium channelPANTHERPTHR11690AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATEDcoord: 10..539
NoneNo IPR availableGENE3DG3DSA:2.60.470.10coord: 66..486
e-value: 3.4E-37
score: 130.3
NoneNo IPR availableGENE3DG3DSA:1.10.287.770coord: 36..516
e-value: 3.4E-37
score: 130.3
NoneNo IPR availableGENE3DG3DSA:1.10.3590.10coord: 89..243
e-value: 3.4E-37
score: 130.3
NoneNo IPR availableGENE3DG3DSA:1.10.287.820coord: 281..385
e-value: 3.4E-37
score: 130.3
NoneNo IPR availableTMHMMTMhelixcoord: 38..57
NoneNo IPR availableTMHMMTMhelixcoord: 500..522